CN104721337A - Traditional Chinese medicine composition, preparation method and application thereof - Google Patents
Traditional Chinese medicine composition, preparation method and application thereof Download PDFInfo
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Abstract
The invention provides a traditional Chinese medicine composition, which comprises 10-30 parts of pasque flower, 3-30 parts of coptis, 3-30 parts of root of large-flowered skullcap and 5-15 parts of fructus chebulae. The traditional Chinese medicine composition has good treatment effect for intestinal infectious diseases due to pathogenic escherichia coli, salmonella and pasteurella multocida.
Description
Technical field
The present invention relates to a kind of Chinese medicine composition, specifically, relate to a kind of Chinese medicine composition being used for the treatment of fowl bacterial disease, with and its preparation method and application.
Background technology
Along with China's aquaculture is gradually to scale, intensive, modernization development; but also there are some problems simultaneously; the sickness rate of such as livestock and poultry rises year by year; new disease constantly increases; especially the disease endangering fowl poultry safety presents the trend of rapid spread, becomes the significant obstacle of puzzlement China aquaculture.Especially the bacteriological infection such as escherichia coli, Salmonella, pasteurellosis bacillus, staphylococcus, is endanger the breeding production bacterial disease that especially broiler production is the most serious at present.M & M remains high, and causes serious economic loss.Current treatment chicken colibacillosis mainly adopts anti-inflammation class Western medicine, although have certain curative effect, but there is the problem easily developing immunity to drugs and have adverse side effect, and remain in the chemicals in Carnis Gallus domesticus, egg, bring potential threat also to the health of the mankind.Modern pharmacology research finds: many Chinese herbal medicine have the effect such as antibacterial, antiinflammatory, antipyretic, antiviral, adjustment immunity of organisms, also have that toxic and side effects is little, without advantages such as Drug resistance, multicomponent Synergistics, thus the research of natural drug more and more comes into one's own.For the diseases prevention and treatment of poultry, have broad application prospects.Although the Chinese herbal and crude drugs preparations kind being used for the treatment of chicken colibacillosis commercially available is at present a lot, practical effect is unsatisfactory.
Publication number be CN101856416A patent discloses a kind of Chinese herbal medicament oral liquid for the treatment of chicken colibacillosis, its raw material weight proportioning is: Radix Pulsatillae 4-6 part, Rhizoma Coptidis 2-4 part, Radix Scutellariae 2-4 part, Cortex Phellodendri 1-3 part, Cortex Fraxini 1-3 part, Radix Paeoniae Alba 1-3 part, Fructus Mume 1-3 part, Fructus Chebulae 1-2 part, Radix Codonopsis 1-3 part, Radix Astragali 1-3 part, Radix Glycyrrhizae 1-2 part.But composition is more in this prescription, quality standard is not easy to control; In prescription, each composition is more, and cost is higher; And antibacterial effect is general.
Summary of the invention
For the problem of current antibacterial to Western medicine drug resistance and drug residue, the invention provides a kind of effective Chinese medicine composition, the poultry diarrhea caused by environmental factorss such as cause of disease, feeding and management such as escherichia coli can be treated, this Chinese medicine composition make oral liquid or make powder by spraying dry, tabletting makes granule dosage form, facilitates administration.
Main purpose of the present invention is to provide a kind of Chinese medicine composition, and wherein, the raw material of described Chinese medicine composition comprises Radix Pulsatillae 10-30 part, Rhizoma Coptidis 3-30 part, Radix Scutellariae 3-30 part and Fructus Chebulae 5-15 part.
Preferably, the raw material of described Chinese medicine composition comprises Radix Pulsatillae 20-30 part, Rhizoma Coptidis 10-30 part, Radix Scutellariae 10-30 part and Fructus Chebulae 10-15 part.
More preferably, the raw material of described Chinese medicine composition comprises the Radix Pulsatillae 30 parts, Rhizoma Coptidis 30 parts, Radix Scutellariae 30 parts and Fructus Chebulae 15 parts.
More preferably, the raw material of described Chinese medicine composition comprises the Radix Pulsatillae 30 parts, Rhizoma Coptidis 20 parts, Radix Scutellariae 20 parts and Fructus Chebulae 15 parts.
Preferably, described Chinese medicine composition is oral liquid, powder and granule.
Another object of the present invention is to the preparation method providing a kind of Chinese medicine composition, described method comprises: soaked by the starting material with water of Chinese medicine composition according to claim 1, then heating extraction; Described starting material with water after extraction soaks, then heating extraction; By the extracting solution mixing described in twice, concentrate, get described supernatant after precipitate with ethanol, prepare the dosage form of described Chinese medicine composition.
Particularly, described preparation method comprises: the Radix Pulsatillae, Rhizoma Coptidis, Radix Scutellariae and Fructus Chebulae are pulverized, and crosses 20-80 mesh sieve.The distilled water immersion 6-8h of above raw material first time 3-5 times amount, then regulate pH value 4.0-5.0 by the NaOH solution of 2%, 6-12h is extracted in the water-bath being placed in 60-80 DEG C, 4-6 layer filtered through gauze.Second time adds the distilled water of 3-5 times amount, and 6h is extracted in the water-bath being then placed in 60-80 DEG C.Leachate is concentrated to 1/5 of its volume.Be placed in the environment of 4 DEG C, the leachate ethanol precipitate with ethanol 12-24h containing 50%-90% of 3-5 times of weight, by 4-6 layer filtered through gauze, gets supernatant.The gelatin of 2-5%, the beta-schardinger dextrin-of 2-5% is added in supernatant.Filtrate is concentrated into the clear paste that relative density is 1.06-1.08.Concentrated solution vacuum spray drying device spraying dry, subpackage.
Another object of the present invention is to provide described Chinese medicine composition treat in preparation and prevent the application in the medicine of fowl bacterial disease.
Preferably, the disease that described fowl bacterial disease comprises escherichia coli, Salmonella, pasteurellosis bacillus, staphy lococcus infection cause.
Preferably, described in 100ml, Chinese medicine composition oral liquid is watered 25kg, 2 times on the one, is used in conjunction 5 days.
The present invention has following technique effect:
1. reasonable recipe, can Synergistic, has stronger broad-spectrum antiseptic.
2. the present invention is pure Chinese medicinal preparation, Fructus Chebulae in prescription and Rhizoma Coptidis have inhibitory action to various bacteria, coordinate the Radix Pulsatillae to have heat-clearing and toxic substances removing, relieving diarrhea with astringents simultaneously, especially there is good curative effect to damp-heat diarrhea, because feeding and management is improper, bacterial diarrhoea is caused to the escherichia coli, Salmonella etc. of fowl, watery diarrhea has obvious therapeutical effect.Clinical trial shows, this product can reach more than 90% to colibacillary control rate, can reach more than 97% to causing the rate that prevents of falling ill because feeding and management is improper.
3. pharmaceutical composition toxicity of the present invention is little, and cost is lower, after making powder with spray dryer spraying dry, can directly be added in drinking water for animals or spice, easy to use, the preventive effect to bacterial disease can be played, simultaneously also can the resistivity of enhancing body.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is described in detail.
Embodiment 1: the preparation of Chinese medicine composition oral liquid of the present invention
Traditional Chinese medicine composition formula of the present invention is in table 1.
The concrete formula of table 1 Chinese medicine composition
| Chinese medicine | Compositions 1(part) | Compositions 2(part) | Compositions 3(part) |
| The Radix Pulsatillae | 15 | 30 | 30 |
| Rhizoma Coptidis | 15 | 30 | 20 |
| Radix Scutellariae | 15 | 30 | 20 |
| Fructus Chebulae | 15 | 15 | 15 |
1. weigh Chinese medicine according to the concrete formula of Chinese medicine composition in table 1, Ingredients Weight is in table 2.
The each Ingredients Weight of table 2 Chinese prescription
| Chinese medicine | Compositions 1(g) | Compositions 2(g) | Compositions 3(g) |
| The Radix Pulsatillae | 100 | 115 | 141.2 |
| Rhizoma Coptidis | 100 | 115 | 94.1 |
| Radix Scutellariae | 100 | 115 | 94.1 |
| Fructus Chebulae | 100 | 57.5 | 70.6 |
2. the Radix Pulsatillae, Rhizoma Coptidis, Radix Scutellariae and Fructus Chebulae pulverize, and cross 60 mesh sieves.
3. above compositions soaks 6h in the beaker of 2000ml water, is placed on the extraction 12h of 80 DEG C, filters.
4. add the water of 2000ml in residue, be placed on the extraction 6h of 80 DEG C, be concentrated to 800ml.Be placed in the environment of 4 DEG C, add the ethanol that 2400ml contains 90% in concentrated solution, precipitate with ethanol 24h, filter, get supernatant.
5. get supernatant concentrating under reduced pressure and reclaim ethanol, being concentrated into 400ml.
6. concentrated solution boils the Tween 80 adding 1% in above-mentioned 5, adds 5%101 clarifier 32ml, the nipalgin of 0.5%, mixing, and evacuation crosses the filter membrane of 0.65 μm, fill sterilizing.
Embodiment 2: the preparation of Chinese medicine powder
1. weigh Chinese medicine according to the concrete formula of Chinese medicine composition in table 1.
2. the Radix Pulsatillae, Rhizoma Coptidis, Radix Scutellariae and Fructus Chebulae pulverize, and cross 60 mesh sieves.
3. soak 6h in the beaker of above compositions 2000ml distilled water, be placed in the water-bath of 80 DEG C and extract 12h, filter.
4. add the distilled water of 2000ml in residue, be placed in the water-bath of 80 DEG C and extract 6h, be concentrated to 800ml.Be placed in the environment of 4 DEG C, add 2400ml in concentrated solution containing the ethanol precipitate with ethanol 24h of 90%, by 6 layers of filtered through gauze, get supernatant.
5. get supernatant concentrating under reduced pressure and reclaim ethanol, being concentrated into 400ml.
7. carry out spraying dry, subpackage with vacuum spray drying device.
Embodiment 3: the preparation of Chinese medicinal composition granules
1. weigh Chinese medicine according to the concrete formula of Chinese medicine composition in table 1.
2. the Radix Pulsatillae, Rhizoma Coptidis, Radix Scutellariae and Fructus Chebulae pulverize, and cross 60 mesh sieves.
3. soak 6h in the beaker of above compositions 2000ml distilled water, be placed in the water-bath of 80 DEG C and extract 12h, 4 layers of filtered through gauze.
4. add the distilled water of 2000ml in residue, be placed in the water-bath of 80 DEG C and extract 6h, be concentrated to 800ml.Be placed in the environment of 4 DEG C, add 2400ml in concentrated solution containing the ethanol precipitate with ethanol 24h of 90%, by 6 layers of filtered through gauze, get supernatant.
5. get supernatant concentrating under reduced pressure and reclaim ethanol, being concentrated into 400ml.
6. according to adding the gelatin of 2%, the beta-schardinger dextrin-of 2% in concentrated solution in step 5.
7. carry out spraying dry with vacuum spray drying device.
8. in Chinese medicine powder, add lactose 9g, magnesium stearate 0.2g.
9. press sheet after mixing, this pre-tabletting is broken in oscillating granulator, and granulate, with 30 mesh sieve removing fine powders, makes uniform particles.
Embodiment 4: the colibacillary clinical trial of artificial infection chicken
The preparation of 1 bacterium liquid
The escherichia coli O of picking rejuvenation
78(purchased from China Veterinery Drug Inspection Office), be inoculated in broth bouillon 37 DEG C and cultivate 24h, carry out count plate, being mixed with bacteria concentration is 1 × 10
9the bacterium liquid of cfu/ml.
2 laboratory animal groupings
Experimental chicken raises 24 ages in days, after checking body condition phenomenon without exception, by its random packet, be divided into Chinese medicine composition 1(first group), Chinese medicine composition 2(second group), Chinese medicine composition 3(the 3rd group), ofloxacin drug control group (the 4th group), the Radix Pulsatillae are fallen apart matched group (the 5th group), negative control group (the 6th group) and normal healthy controls group (the 7th group), often organize 20.
3 counteracting toxic substances and administration
Except normal healthy controls group, all the other are group thoracic muscle injection bacterium liquid 0.2ml/ respectively, Chinese drug-treated group is after counteracting toxic substances morbidity (after counteracting toxic substances 24h), first group gavages oral liquid 0.5ml in 1g/ml Chinese medicine composition 1, second group gavages oral liquid 0.5ml in 1g/ml Chinese medicine composition 2,3rd group gavages oral liquid 0.5ml in 1g/ml Chinese medicine composition 3, at each 1 time of upper and lower noon, is used in conjunction 5d.
Western medicine drug control group gavages enrofloxacin, according to every 1L water, and chicken 50mg.2 times on the one, be used in conjunction 5d(with reference to veterinary drug guide for use chemical drugs volume), Chinese medicine matched group gavages the Radix Pulsatillae and falls apart, 1g(crude drug)/ml, gavage 0.5ml(with reference to powder stick in veterinary drug guide for use).
4 efficacy determinations
Morbidity: test chicken occurs that spirit is depressed successively, and appetite detracts, and squats or crowded in heaps, and two wings are sagging, is judged as morbidity.
Syndrome Scale: clinical symptoms score standard is in table 3.
Table 3 clinical symptoms score standard
| rank | mark | symptom |
| one-level | 0 | the chicken mental status is good, drinking-water and appetite normal; Behavioral activity is normal. |
| secondary | 1 | squat or crowded in heaps, two wings are sagging, drive to leave rapidly afterwards. |
| three grades | 2 | peel off slow-witted vertical, eye closing lethargy, drives rear bradykinesia. |
| level Four | 3 | spirit is extremely depressed drives reactionless, does not rise or dead sleepingly. |
Observation index: by mortality rate, cure rate, effective percentage, body weight increase rate
Mortality rate: all at duration of test, all dead chickens, escherichia coli classical symptom is had through cuing open inspection, the dead chicken liver of aseptic collection, and differentiate to cultivate through Mai Kangkai culture medium, isolate colibacillary chicken, be judged as the dead chicken that escherichia coli are lethal, account for the ratio of whole test group chicken number with the dead chicken number that escherichia coli are lethal, calculate mortality rate.
Cure rate: treatment group, the chicken mental status, appetite recover normal, and transference cure, scores 0 point, and aseptic collection chicken liver, differentiate to cultivate through Mai Kangkai culture medium and do not isolate colibacillary chicken, be judged as curing.Account for the ratio of whole test group according to healing chicken number, calculate cure rate.
Effective percentage: after medication, spirit, the appetite of test chicken, to take an evident turn for the better, symptom alleviates, by every chicken score record result when every chicken score record result and off-test before administration, reduce with integration and be more than or equal to 1, be judged as that effectively effective chicken quantity accounts for the ratio of whole test group chicken quantity, calculate effective percentage.
Body weight increase rate: according to during on-test and off-test time every chicken body weight, calculate average weight gain amount, then calculate the average weight gain often organizing chicken, with the weightening finish of blank group for 100%, each group weightening finish by comparison each group of body weight increase rate.
Computing formula:
5 result of the tests
As shown in Table 4, after counteracting toxic substances 24h, the sickness rate 90%, 91.2%, 89.8%, 91.8%, 90.2%, 91.7% of each group (1-6 group), group difference is not remarkable.In the present invention, Chinese medicine composition can effectively reduce the mortality rate of chicken group, and the effective percentage in Chinese medicine composition 2 reaches 95%.In compositions 2 compared with negative control group, improve the body weight increase rate 19.19% of chicken group.
Table 4 Chinese medicine composition is on the impact of chicken colibacillosis therapeutic effect
Note: the different lowercase alphabet of shoulder mark shows significant difference (P<0.05).
6 conclusions
In the present invention, different Chinese medicine composition significantly can reduce the mortality rate of ehec infection chicken group, improves the rate of body weight gain of chicken group.Conclusion: Chinese medicine composition 2 oral liquid gavaging 0.5ml, at each 1 time of upper and lower noon, being used in conjunction 5d has certain therapeutic effect to colibacillosis.
Embodiment 5: Chinese medicine composition oral liquid is to chicken colibacillosis therapeutic test
1 test method
Select 37 age in days chicken, 2000 broiler that nature is ill, be divided into 5 groups, often organize 400.First group is No. 2 formula high dose group in embodiment 1, and usage is that 200ml Chinese medicine oral liquid is watered 25kg, 2 times on the one, is used in conjunction 5 days.
2nd group is dosage group in No. 2 formula in embodiment 1, and usage is that 100ml Chinese medicine oral liquid is watered 25kg, 2 times on the one, is used in conjunction 5 days.
3rd group is No. 2 formula low dose group in embodiment 1; Usage is that 50ml Chinese medicine oral liquid is watered 25kg, 2 times on the one, is used in conjunction 5 days.
4th group of Western medicine drug control group gavages enrofloxacin, according to every 1L water, and chicken 50mg.2 times on the one, be used in conjunction 5d(with reference to veterinary drug guide for use chemical drugs volume),
5th group of Chinese medicine matched group gavages the Radix Pulsatillae and falls apart, 1g(crude drug)/ml, gavage 0.5ml(with reference to powder stick in veterinary drug guide for use).
Observe the clinical symptoms of chicken group, cure rate and mortality rate.
2 test results and analysis
Clinical symptom: initial spirit is depressed, and have loose bowels, abdominal part expands, after fragmentary dead.Cut open inspection pathological change: abdominal part has yellow liquid, duodenum intestinal wall is thinning, and liver surface has one deck cellulose membrane.Gather chicken liver, differentiate that culture of isolated goes out escherichia coli through Mai Kangkai culture medium, be diagnosed as escherichia coli.
It is reported due to weather and environment feeding and management is improper causes chicken to mass-send raw colibacillosis.
As shown in Table 5, in the present invention, the cure rate of Chinese medicine oral liquid to chicken colibacillosis of Chinese medicine composition 2 reaches 97%-99%, can effectively reduce the mortality rate of chicken group.
Table 5 traditional Chinese medicine particle is on the impact of the therapeutic effect of chicken colibacillosis
3 conclusions
Dosage group in No. 2 formula in embodiment 1, usage is that 100ml Chinese medicine oral liquid is watered 25kg, 2 times on the one, is used in conjunction 5 days, has certain therapeutic effect to the colibacillosis of chicken group, and its effect is better than enrofloxacin matched group and the Radix Pulsatillae and falls apart matched group.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (10)
1. a Chinese medicine composition, wherein, the raw material of described Chinese medicine composition comprises Radix Pulsatillae 10-30 part, Rhizoma Coptidis 3-30 part, Radix Scutellariae 3-30 part and Fructus Chebulae 5-15 part.
2. Chinese medicine composition according to claim 1, wherein, the raw material of described Chinese medicine composition comprises Radix Pulsatillae 20-30 part, Rhizoma Coptidis 10-30 part, Radix Scutellariae 10-30 part and Fructus Chebulae 10-15 part.
3. Chinese medicine composition according to claim 2, wherein, the raw material of described Chinese medicine composition comprises the Radix Pulsatillae 30 parts, Rhizoma Coptidis 30 parts, Radix Scutellariae 30 parts and Fructus Chebulae 15 parts.
4. Chinese medicine composition according to claim 2, wherein, the raw material of described Chinese medicine composition comprises the Radix Pulsatillae 30 parts, Rhizoma Coptidis 20 parts, Radix Scutellariae 20 parts and Fructus Chebulae 15 parts.
5. the Chinese medicine composition according to any one of Claims 1 to 4, wherein, described Chinese medicine composition is oral liquid, powder and granule.
6. a preparation method for Chinese medicine composition, described method comprises:
The starting material with water of Chinese medicine composition according to claim 1 is soaked, then heating extraction;
Described starting material with water after extraction soaks, then heating extraction;
By the extracting solution mixing described in twice, concentrate, get described supernatant after precipitate with ethanol, prepare the dosage form of described Chinese medicine composition.
7. the Chinese medicine composition according to any one of Claims 1 to 4 is treated in preparation and is prevented the application in the medicine of fowl bacterial disease.
8. Chinese medicine composition according to claim 5 is treated in preparation and is prevented the application in the medicine of fowl bacterial disease.
9. application according to claim 7, the disease that wherein, described fowl bacterial disease comprises escherichia coli, Salmonella, pasteurellosis bacillus, staphy lococcus infection cause.
10. application according to claim 7, wherein, described in 100ml, Chinese medicine composition oral liquid is watered 25kg, 2 times on the one, is used in conjunction 5 days.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108619293A (en) * | 2018-08-09 | 2018-10-09 | 集美大学 | A kind of composition and its preparation method and application being combined treatment grouper vibrio fluvialis disease using Chinese and Western medicine |
| CN109568560A (en) * | 2019-01-28 | 2019-04-05 | 孟繁华 | A kind of preparation method of active compound that treating the infection of Escherichia coli persister |
| CN112168888A (en) * | 2020-11-12 | 2021-01-05 | 邓云波 | Pure traditional Chinese medicine composition powder for treating bacterial and viral intestinal diseases of livestock and poultry |
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2013
- 2013-12-18 CN CN201310706559.1A patent/CN104721337B/en active Active
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108619293A (en) * | 2018-08-09 | 2018-10-09 | 集美大学 | A kind of composition and its preparation method and application being combined treatment grouper vibrio fluvialis disease using Chinese and Western medicine |
| CN109568560A (en) * | 2019-01-28 | 2019-04-05 | 孟繁华 | A kind of preparation method of active compound that treating the infection of Escherichia coli persister |
| CN112168888A (en) * | 2020-11-12 | 2021-01-05 | 邓云波 | Pure traditional Chinese medicine composition powder for treating bacterial and viral intestinal diseases of livestock and poultry |
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