CN101085048B - Traditional Chinese medicine for detoxicating for animals and preparation method thereof - Google Patents
Traditional Chinese medicine for detoxicating for animals and preparation method thereof Download PDFInfo
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- CN101085048B CN101085048B CN2006100179106A CN200610017910A CN101085048B CN 101085048 B CN101085048 B CN 101085048B CN 2006100179106 A CN2006100179106 A CN 2006100179106A CN 200610017910 A CN200610017910 A CN 200610017910A CN 101085048 B CN101085048 B CN 101085048B
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Abstract
The invention relates to a detoxicating medicament for animals and its preparing process, wherein the medicament comprises the following components (by weight ratio): radix isatidis 3-6 parts, giant knotweed rhizome 3-6 parts, astragalus root 3-5 part, kuh-seng 2-5 parts, Chinese ephedra 7-10 parts, licorice 2-4 parts.
Description
Technical field
The present invention relates to a kind of detoxicating for animals Chinese medicine, simultaneously, also relate to a kind of this detoxicating for animals preparation method of Chinese medicine.
Background technology
Aviculture is to intensifying, when the scale direction develops, often having the popular of various infectious disease, and wherein viral infectious is a kind of disease that develops rapidly, is difficult to cure, and accounts for 1/4 of fowl diseases, causes enormous economic loss every year.Viral disease is in epidemiological process, hereditary variation often takes place, many atypical infection and morbidity appear, some virus can be incorporated on the host chromosome with the form of viroid, in case and host resistance descends, it can discharge virion again, and the harm host causes the host to fall ill even death.Because the extensive existence of virus uses disinfectant to carry out disinfection to environment, can not treat the morbidity birds, though implement epidemic prevention certain effect is arranged, because many serotypes of virus also often cause immuning failure.At present, though it is a lot of to be used for the treatment of the Western medicine of animal viral disease, because life-time service and using dosage continue to increase, the virus even the infection that occurs making a variation are given human, there is serious harm in life to the mankind, and the effect of Western medicine is very limited, and has caused drug residue.
Summary of the invention
The object of the present invention is to provide a kind of detoxicating for animals Chinese medicine, to reach to viral disease prevention and treatment double effects.
Simultaneously, the present invention also aims to provide a kind of detoxicating for animals preparation method of Chinese medicine.
To achieve these goals, technical program of the present invention lies in having adopted a kind of detoxicating for animals Chinese medicine, comprise the composition of following parts by weight: Radix Isatidis 3-6 part, Rhizoma Polygoni Cuspidati 3-6 part, Radix Astragali 3-5 part, Radix Sophorae Flavescentis 2-5 part, Herba Ephedrae 7-10 part, Radix Glycyrrhizae 2-4 part.
Chinese medicine of the present invention can be any one dosage form.
Chinese medicine preferred particulates of the present invention agent, its adjuvant can be glucose.
Simultaneously; technical program of the present invention also lies in having adopted a kind of this detoxicating for animals preparation method of Chinese medicine; may further comprise the steps: take by weighing the Radix Isatidis that weight portion is 3-6 part respectively; the Rhizoma Polygoni Cuspidati of 3-6 part; the Radix Astragali of 3-5 part; the Radix Sophorae Flavescentis of 2-5 part; the Radix Glycyrrhizae of the Herba Ephedrae of 7-10 part and 2-4 part; place in the extraction pot; and place the water that 8-12 doubly measures; low temperature (≤70 ℃) dynamic vacuum is extracted and is concentrated; carry to color light; stop to extract; medicinal liquid is concentrated to the extractum of relative density 1.20-1.25 (70 ℃ of heat are surveyed), gets extractum, and it is an amount of to add the adjuvant glucose; with airpillow-dry granulator mixing granulation, drying.
Particle diameter after described Chinese medicine is granulated is the 14-60 mesh sieve.
In each raw material among the present invention: Radix Isatidis, hardship, cold.GUIXIN, stomach warp have heat-clearing and toxic substances removing, removing heat from blood, the effect of sore-throat relieving contains multiple compositions such as indigo, indirubin, Palmic acid, and multiple gram positive bacteria, gram negative bacteria and influenza virus are all had inhibitory action, improving immunocompetence has and significantly separates thermal effect; Rhizoma Polygoni Cuspidati: little hardship, be slightly cold, return liver, gallbladder, lung meridian, effect with expelling wind and removing dampness, eliminating stasis to stop pain, relieving cough and resolving phlegm, contain polygonin, flavonoid, emodin, physcione, polysaccharide, various bacteria such as staphylococcus aureus, bacillus pyocyaneus all there is certain inhibitory action, virus is also had inhibitory action.Herba Ephedrae: hot, little hardship, temperature, return lung, urinary bladder channel, main component is an ephedrine, and containing a spot of pseudoephedrine, volatile oil, flavone compound, ephedran etc., it has the effect of depressed lung-energy dispersing, inducing diuresis to remove edema, the key medicine of breathing with cough for treatment, with clearing lung, relieving asthma, as Maxing Ganshi Tang (treatise on Febrile Diseases), and influenza virus had inhibitory action, normal and Radix Glycyrrhizae is joined.The Radix Astragali: sweet, tepor, has the effect that tonifying Qi and lifting yang, benefit are defended consolidating superficial resistance, inducing diuresis to remove edema, poison holding life, mainly contain glycoside, polysaccharide, flavone, aminoacid, trace element etc., can promote metabolism, the resisting fatigue of body, the renewal of promotion hepatic protein, tangible diuresis is arranged, can strengthen and the adjusting body's immunological function, can improve the premunition of body.Radix Sophorae Flavescentis: bitter, cold, nontoxic, contain multiple alkaloids such as matrine, oxymatrine, Iosmatrine, dysentery bacterium, escherichia coli are all had in various degree inhibitory action, and diuresis, antiinflammatory are arranged, effect such as relieving asthma; Have heat clearing and damp drying diuretic function, cure mainly toxic-heat and blood stasis, jaundice, the pouring of damp and hot urine.Radix Glycyrrhizae: nature and flavor are sweet, flat, GUIXIN, lung, spleen, stomach warp.Invigorating the spleen and replenishing QI, heat-clearing and toxic substances removing, expelling phlegm for arresting cough, coordinating the actions of various ingredients in a prescription.Be leguminosae, its main component has triterpenes, flavonoid, alkaloid, polysaccharide etc., the flat sweet in the mouth of property, effects such as energy invigorating the spleen and replenishing QI, heat-clearing and toxic substances removing, coordinating the actions of various ingredients in a prescription, Radix Glycyrrhizae extractum is controlled peptic ulcer, viral hepatitis has good effect, " book on Chinese herbal medicine converges and says ": " with middle QI invigorating, the medicine of tonify deficiency detoxifcation also." traditional traditional Chinese medical science thinks that virus disease is many because of the deficiency of vital energy causes, astragalus polysaccharides, Radix Glycyrrhizae all have the tonify deficiency effect, so the two compatibility has the good curing effect to viral disease.The present invention is guidance with the theory of Chinese medical science, draws the prescription of Chinese traditional treatment viral disease, and all medicines share has heat-clearing and toxic substances removing, the effect of relieving cough and asthma.Chinese medicine of the present invention is taken into account prevention and treatment, can effectively prevent the system avian viral disease, as infectious bronchitis, infectious laryngotracheitis etc.
Clinical test results shows: Chinese medicine of the present invention mixes drink by every 1L water 0.5g, and logotype 4 days can significantly improve the effective percentage to chicken virus, and infectious bronchitis of chicken, infectious laryngotracheitis are had good effect of control.
Chinese medicine of the present invention has following characteristics:
(1) prevention and treatment double effects are arranged, and useful prescription just, and consumption is few, short treating period, cure rate height, does not influence advantages such as fertility performance.
(2) the experiment clinical test results shows: Chinese medicine of the present invention mixes drink by every 1L water 0.5g, and logotype 4 days can obtain excellent curative to infectious laryngotracheitis of chicken.
(3) the animal safety test shows, Chinese medicine of the present invention 5 times of amounts of dosage routinely uses, and logotype 4 days is good to the safety of chicken.
Evidence: Chinese medicine of the present invention develops in a healthy way to China animal husbandry and has played positive role, not only has effects such as antibacterial, sterilization, antiviral, and can regulate body's immunity, has significant health care, cures the disease, diseases prevention, growth promoting function.In addition, herbal medicine preparation special effect of the present invention, have no side effect, noresidue, nuisanceless, the fertility performance that do not influence animal, do not develop immunity to drugs, and aboundresources, cheap, drug cost is starkly lower than the chemicals preparation, be to strengthen its market competitiveness from now on, better bring into play social benefit, and bring economic benefit to society.
1, stability of formulation test
1.1 accelerated test
Accelerated test result shows: placed 6 months under the accelerated test condition, every quality investigation project has no significant change, and illustrates that Chinese medicine of the present invention has good stability, and packaging material can well protect this product quality not to be affected by the external environment.
1.1.1 acceleration by light test
The acceleration by light result of the test shows: Chinese medicine of the present invention was placed 10 days under illuminance 4500 ± 500LX condition, and every quality index has no significant change, and illustrated that this product is insensitive to light, and lucifuge, airtight preservation more can effectively be protected its quality.
2.1 long term test
Long-term test results shows: Chinese medicine of the present invention places 25 ± 2 ℃ of temperature; The FR-SP growth cabinet of relative humidity 60% ± 5% was placed 24 months, and every quality investigation project has no significant change, and illustrated that this product can preserve 2 years at least under the condition of long term test, so the effect duration of this product is decided to be 2 years.
Clinical trial
Chinese medicine of the present invention is to the therapeutic test of experimental infectious laryngotracheitis of chicken
Adopt the method for artificial vaccination infectious laryngotracheitis virus strain, duplicate infectious laryngotracheitis of chicken, by the clinical administration administration of recommending, observe physiological situation and the incidence of animal after the administration, the record death toll divides healing, produce effects, index such as effective, invalid that the result is added up and estimates.Result of the test shows: Chinese medicine of the present invention mixes drink by every 1L water 0.5g, and logotype 4 days can significantly improve effective percentage, and infectious laryngotracheitis of chicken is had excellent curative.
Chinese medicine of the present invention has heat-clearing and toxic substances removing, function of relieving cough and calming asthma.Be used for the treatment of and prevent animal viral disease, as infectious bronchitis, infectious laryngotracheitis etc.For science, estimate Chinese medicine of the present invention that this research group produces clinical efficacy objectively to infectious laryngotracheitis of chicken, make therapeutic test have certain representativeness and clinical meaning, the spy carry out this test.
1, materials and methods
1.1 for the reagent product
Chinese medicine of the present invention.
1.2 reagent
Hydrochloric acid, sodium hydroxide, sodium chloride, potassium dihydrogen phosphate etc. are homemade analytical pure
The healthy Embryo Gallus domesticus of 9-10 age in days Luoyang Pu Laike SPF Animal House provides.
1.3 animal subject
180 of 30 age in days Luo Man chickens, body weight 231g ± 10g, Luoyang Pu Laike SPF Animal House provides, and without the infectious laryngotracheitis immunity, experimental animal is divided into 6 groups at random, and several 30 of every treated animal is raised in cages, and all feeds not contain the complete feed of medicine.
1.4 cause of disease
Infectious laryngotracheitis of chicken ILT/13 strain is derived from China Veterinery Drug Inspection Office.
1.5 artificial onset
The strong toadstool kind that to preserve earlier is inoculated in the 9-10 day instar chicken embryo, and the chorioallantoic membrane that reached for 16 generations through Embryo Gallus domesticus is malicious by force, to the median infective dose (ID of chicken
50) be the strong malicious 1ml of chorioallantoic membrane of 0.1% dilution.Except that the blank group, inject 2% the strong malicious 0.5ml of chorioallantoic membrane in every chicken trachea.
Clinical settings such as inoculation back is observed the spirit of respectively organizing test chicken, searches for food, drunk water, breathing; The chicken that dies of illness is carried out pathology immediately cut open inspection, bacterial isolate is cultivated from trachea.
1.6 therapeutic trial
Behind chicken inoculation laryngotracheitis virus 16-30 hour, show as depressed, the dyspnea of spirit, stridulate, cough, shed tears, typical clinical symptom such as persistence rhinorrhea, test subsequently is divided into 6 groups at random, several 30 of every treated animal, as shown in table 1, press the medication of table 1 dosage, logotype 4 days this moment.
The clinical symptoms change that begin treatment is observed chicken in two weeks is cutd open inspection and pathogen separation to the chicken that dies of illness.
Grouping of table 1 test chicken and processing
1.7 therapeutic evaluation index
Cure: clinical symptoms complete obiteration spirit and diet desire recover normal for curing after the medication treatment, and statistics is cured number and calculated cure rate;
Produce effects: obviously alleviate spirit and diet desire improvement person is the treatment produce effects, statistics produce effects number calculating obvious effective rate through medication treatment back clinical symptoms;
Effectively: be cure rate and obvious effective rate sum;
Invalid: through medication treatment back symptom do not disappear the state of an illness do not take a turn for the better and treat during because of primary disease death person all is considered as invalidly, add up invalid number, the calculating inefficiency;
Average weight gain: according to the body weight of every chicken before the administration and during off-test, calculate the gain in weight of every chicken, calculate every group of average weight gain in view of the above;
The relative weight gain rate: the relative weight gain rate is to calculate by each the medication group and the ratio of the average weight gain of blank group.
1.8 the statistical disposition of data
Carry out the significance test of data with biometrics, wherein effective percentage is with card side (X
2) check; Weightening finish is checked with t.
2, result
2.1 the treatment situation and the result of the test of infectious laryngotracheitis of chicken
Behind the test chicken counteracting toxic substances, show as depressed, the dyspnea of spirit, stridulate, cough, shed tears, typical clinical symptom such as persistence rhinorrhea.30 chickens of positive controls, dead 19, after inoculation 1-7 days death time.Cut open the sick typical cytopathic of the inspection chicken that dies of illness with infectious laryngotracheitis of chicken, separable to infectious laryngotracheitis from the chicken trachea of dying of illness.After each medication group medication, clinical symptoms alleviates gradually, in two weeks after the beginning medication, tests every index and sees Table 2:
Table 2 Chinese medicine of the present invention is to the curative effect of infectious laryngotracheitis of chicken
2.2 statistical analysis
Chinese medicine height of the present invention, middle dosage group are brought out effective percentage and the poor heteropole of positive controls remarkable (P＜0.01) of infectious laryngotracheitis chicken to the artificial challenge; Chinese medicine low dose group of the present invention is brought out effective percentage and the positive controls comparing difference remarkable (0.01＜P＜0.05) of infectious laryngotracheitis chicken to the artificial challenge; The effective percentage of Chinese medicine height of the present invention, middle dosage group is significantly higher than Radix Isatidis groups of grains (0.01＜P＜0.05); The effective percentage of the Chinese medicine low dose group of the present invention difference not significantly (P＞0.05) of comparing with the Radix Isatidis groups of grains; The effective percentage of Chinese medicine height of the present invention, middle dosage group is significantly higher than low dose group (0.01＜P＜0.05).
Chinese medicine height of the present invention, middle dosage group are brought out the relative weight gain rate and the poor heteropole of positive controls remarkable (P＜0.01) of infectious laryngotracheitis chicken to the artificial challenge; The relative weight gain rate of Chinese medicine height of the present invention, middle dosage group is significantly higher than Radix Isatidis groups of grains (0.01＜P＜0.05), and the difference of comparing with the blank group is remarkable (P＞0.05) not; The relative weight gain rate of Chinese medicine low dose group of the present invention difference not significantly (P＞0.05) of comparing with the Radix Isatidis groups of grains, the significant difference (0.01＜P＜0.05) of comparing with the blank group; The relative weight gain rate of Chinese medicine height of the present invention, middle dosage group is significantly higher than low dose group (0.01＜P＜0.05).
3, conclusion
3.1 Chinese medicine height of the present invention, middle dosage group mix drink infectious laryngotracheitis of chicken is had excellent curative, with other medication group mutually specific energy significantly improve effective percentage, and aspect weightening finish, obviously be better than other medication group and positive controls.
3.2 according to this result of the test, recommend Chinese medicine of the present invention to mix drink by every 1L water 0.5g, logotype 4 days can obtain excellent curative to infectious laryngotracheitis of chicken.
Chinese medicine of the present invention is to the safety testing of chicken
For estimating the safety of Chinese medicine of the present invention to the target animals chicken, the spy carry out this test, reference during for clinical practice.
1, material
1.1 experimental animal
120 of 22 age in days Luo Man chickens, body weight 200g ± 10g, Luoyang Pu Laike SPF Animal House provides.
1.2 be subjected to the reagent thing
Chinese medicine of the present invention.
2, test method
Experimental animal all is divided into four groups at random, every group 30, the 1st group of drinking water is as the blank group, 2nd, 3,4 groups are mixed drink with the various dose of every 1L water 0.5g, 1.5g, 2.5g respectively, every day is observed in logotype 4 days such as chicken response situation (search for food, drink water, spirit, breathing, feces) and death condition etc., observes continuously 6 days, after the off-test, slaughter 5 at random for every group and cut open inspection as pathology.
3, result of the test
Result of the test such as following table are participated in the experiment chicken after increasing dosage, the physiological status of chicken, and the mental status and matched group compare, no abnormal generation.Cut open seizure test group 3 and find that 2 night blindness intestinal have many petechias, in the thin content of red sauce is arranged, microscopy is all found egg capsule, it is dead by coccidiosis making a definite diagnosis, can find out that from each group of whole medication dead irrelevant with medication, each internal organs of other experimental animals there is no obvious pathological changes.
The present invention is to the safety testing of chicken
4, conclusion
Chinese medicine of the present invention is big to the chicken safety, uses to recommend 5 times of amounts of consumption, does not observe obvious toxic and side effects.So clinical mixing by every 1L water 0.5g drunk, logotype 4 days is good to the safety of chicken.
The specific embodiment
Embodiment 1
Detoxicating for animals Chinese medicine in the present embodiment is made up of following composition: Radix Isatidis 3 grams, Rhizoma Polygoni Cuspidati 3 grams, the Radix Astragali 3 grams, Radix Sophorae Flavescentis 2 grams, Herba Ephedrae 7 grams, Radix Glycyrrhizae 2 grams.The Chinese medicine preferred particulates agent of present embodiment; its adjuvant can be glucose; its preparation method may further comprise the steps: the Radix Isatidis that takes by weighing 3 grams respectively; the Rhizoma Polygoni Cuspidati of 3 grams; the Radix Astragali of 3 grams; the Radix Sophorae Flavescentis of 2 grams; the Radix Glycyrrhizae of the Herba Ephedrae of 7 grams and 2 grams; place in the extraction pot; and place the water of 8 times of amounts; low temperature (≤70 ℃) dynamic vacuum extract to concentrate, and carries to color lightly, stops to extract; medicinal liquid is concentrated to the extractum of relative density 1.20 (70 ℃ of heat are surveyed); get extractum, it is an amount of to add the adjuvant glucose, with airpillow-dry granulator mixing granulation; particle diameter is 14 mesh sieves; drying makes the 1g finished product be equivalent to crude drug 2.5g, get final product Chinese medicine of the present invention.
Embodiment 2
Detoxicating for animals Chinese medicine in the present embodiment is made up of following composition: Radix Isatidis 5 grams, Rhizoma Polygoni Cuspidati 5 grams, the Radix Astragali 4 grams, Radix Sophorae Flavescentis 4 grams, Herba Ephedrae 8 grams, Radix Glycyrrhizae 3 grams.The Chinese medicine of present embodiment is granule; its adjuvant can be glucose; its preparation method may further comprise the steps: the Radix Isatidis that takes by weighing 5 grams respectively; the Rhizoma Polygoni Cuspidati of 5 grams; the Radix Astragali of 4 grams; the Radix Sophorae Flavescentis of 4 grams; the Radix Glycyrrhizae of the Herba Ephedrae of 8 grams and 3 grams; place in the extraction pot; and place the water of 9 times of amounts; low temperature (≤70 ℃) dynamic vacuum extract to concentrate, and carries to color lightly, stops to extract; medicinal liquid is concentrated to the extractum of relative density 1.23 (70 ℃ of heat are surveyed); get extractum, it is an amount of to add the adjuvant glucose, with airpillow-dry granulator mixing granulation; particle diameter is 25 mesh sieves; drying makes the 1g finished product be equivalent to crude drug 2.5g, get final product Chinese medicine of the present invention.
Embodiment 3
Detoxicating for animals Chinese medicine in the present embodiment is made up of following composition: Radix Isatidis 6 grams, Rhizoma Polygoni Cuspidati 6 grams, the Radix Astragali 5 grams, Radix Sophorae Flavescentis 5 grams, Herba Ephedrae 10 grams, Radix Glycyrrhizae 4 grams.The Chinese medicine of present embodiment is granule; its adjuvant can be glucose; its preparation method may further comprise the steps: the Radix Isatidis that takes by weighing 6 grams respectively; the Rhizoma Polygoni Cuspidati of 6 grams; the Radix Astragali of 5 grams; the Radix Sophorae Flavescentis of 5 grams; the Radix Glycyrrhizae of the Herba Ephedrae of 10 grams and 4 grams; place in the extraction pot; and place the water of 9 times of amounts; low temperature (≤70 ℃) dynamic vacuum extract to concentrate, and carries to color lightly, stops to extract; medicinal liquid is concentrated to the extractum of relative density 1.25 (70 ℃ of heat are surveyed); get extractum, it is an amount of to add the adjuvant glucose, with airpillow-dry granulator mixing granulation; particle diameter is 40 mesh sieves; drying makes the 1g finished product be equivalent to crude drug 2.5g, get final product Chinese medicine of the present invention.
It should be noted last that: above embodiment is the unrestricted technical scheme of the present invention in order to explanation only, although the present invention is had been described in detail with reference to the foregoing description, those of ordinary skill in the art is to be understood that: still can make amendment or be equal to replacement the present invention, and not breaking away from any modification or partial replacement of the spirit and scope of the present invention, it all should be encompassed in the middle of the claim scope of the present invention.
Claims (4)
1. a stud bird is used detoxicating Chinese medicine, it is characterized in that: made by following medicine materials in portions by weight: Radix Isatidis 3-6 part, Rhizoma Polygoni Cuspidati 3-6 part, Radix Astragali 3-5 part, 25 parts of Radix Sophorae Flavescentiss, Herba Ephedrae 7-10 part, Radix Glycyrrhizae 2-4 part.
2. fowl according to claim 1 is used detoxicating Chinese medicine, it is characterized in that: described Chinese medicine is granule, and its adjuvant is a glucose.
3. a fowl as claimed in claim 1 is used the detoxicating preparation method of Chinese medicine; it is characterized in that: may further comprise the steps: take by weighing the Radix Isatidis that weight portion is 3-6 part respectively; the Rhizoma Polygoni Cuspidati of 3-6 part; the Radix Astragali of 3-5 part; the Radix Sophorae Flavescentis of 2-5 part; the Radix Glycyrrhizae of the Herba Ephedrae of 7-10 part and 2-4 part; place in the extraction pot; and place the water that 8-12 doubly measures; extract and concentrate not being higher than under 70 ℃ the low temperature dynamic vacuum; carry to color light; stop to extract; it is the extractum of 1.20-1.25 that medicinal liquid is condensed into 70 ℃ of heat survey relative densities; get extractum; it is an amount of to add the adjuvant glucose, with airpillow-dry granulator mixing granulation, drying.
4. fowl according to claim 3 is used the detoxicating preparation method of Chinese medicine, it is characterized in that: the particle diameter after the described granulation is the 14-60 mesh sieve.
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| CN104288240B (en) * | 2014-10-16 | 2017-10-27 | 山西天合元动物保健科技有限公司 | A kind of antiviral oral liquor and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1393232A (en) * | 2001-06-25 | 2003-01-29 | 曾洁莹 | 'Jinma' regeneration capsule for detoxicating liver |
| CN1418664A (en) * | 2002-11-25 | 2003-05-21 | 郁建生 | Lireqing injecta (for clearing heat and diminishing inflammation) |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1393232A (en) * | 2001-06-25 | 2003-01-29 | 曾洁莹 | 'Jinma' regeneration capsule for detoxicating liver |
| CN1418664A (en) * | 2002-11-25 | 2003-05-21 | 郁建生 | Lireqing injecta (for clearing heat and diminishing inflammation) |
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