CN104623068A - Medicine for treating infantile fever and convulsion and preparation method of medicine - Google Patents

Abstract

The invention discloses a medicine for treating infantile fever and convulsion. The medicine is prepared from coptis chinensis, pulvis cornus bubali concentrates, cornu gorais, calamitas urinae hominis, fermented soybeans, folium isatidis, schizonepeta, notopterygium roots, roots of kudzu vines, rehmannia, caulis clematidis armandii, roots of common peony, scutellaria baicalensis, common hogfennel roots, radix scrophulariae, platycodon grandiflorum, radix bupleuri, tamarix chinensis, cimicifugae foetidae and burdocks. The medicine has the functions of clearing away heat and toxic materials and relieving the exterior syndrome and eliminating phlegm, is used for treating general fever and cough induced by acute convulsion, typhoid fever, fever at night, hematuria and looming measles as well as dysentery, watery diarrhea, dyspepsia and stomachache, and is remarkable in curative effect and small in side effect.

Description

Medicine for the treatment of fever of children infantile convulsion and preparation method thereof

Technical field

The present invention relates to and a kind ofly treat medicine of fever of children infantile convulsion and preparation method thereof, belong to the field of medicine technology.

Background technology

Infantile convulsion is many is inside accumulate by diseases caused by exogenous pathogenic factor seasonal pathogen epidemic disease, and the hot food stagnation of expectorant and being terrified suddenly cause, and ailment said due to cold or exposure expectorant is stagnant, stops up caused by the meridians the liver pulse contracture.Symptom have order for a long time not under, soon white of the eye, and opisthotonus, pant, aphonia of crowing, appearance is helvolus, holds heat under the arm by nausea, exerts a pernicious influence heart spleen therefore make stiff tongue bluish lip, and poly-mouth sends out the symptoms such as taboo.Hyperpyrexia and infantile convulsion refers to because high thermal conductance causes the faintness of convulsions convulsion, is a kind of disease common clinically, and clinical main manifestations is the tatanic and clonic twitches of whole body or local.Duration of seizure by the several seconds to several minutes, can be lost simultaneously consciously, and severe patient repeatedly or can continue outbreak.Any season all can occur, and is mainly in child, and the age is less, and sickness rate is higher.Its card feelings are often more dangerous, and change rapidly, threatens children's's life.Infantile hyperpyrexia infantile convulsion be because of its nervous system development immature caused by, mainly occur in the children's of 6 months to 3 years old, it is one of common emergency case, can family history be had, much more general to occur when rising from the beginning of, body temperature in disease, heating companion coolness of extremities, easily occur during complexion burnt hair, can recurrent exerbation, cause cerebral anoxia after repeatedly fainting from fear, affect Growth of Intelligence in Children.

Upper respiratory tract infection is disease the most common, often causes heating by diseases caused by exogenous pathogenic factor, and cough expectorant is yellow, asthma, the symptoms such as laryngopharynx swelling and pain.Be common in pediatric patient, fever caused by exogenous pathogens can affect life and the normal development of patient.Traditional Chinese Medicine is thought: the cough caused because of wind heat is " heat syndrome cough ", and cardinal symptom is that expectorant is thick and yellow, and nasal mucus is also with Huang, at this moment with the medicine of cool cold matter, the heat drop in human body will be got off; And cause because of wind and cold just cry " cold cough ", the abundant expectoration of patient is white and thin, and having a running nose also is clear water sample, and such patient just must dispel the cold in body with mild medicine.Cough is the modal a kind of symptom of respiratory tract disease.Cough is a kind of protectiveness defense function of human body.By cough, the secretions of respiratory tract can be discharged or invade endotracheal foreign body.Only there is cough and without the dry cough that is called of expectorant, be found in various diseases.

At present, acute infantile convulsion is treated in market, typhoid fever is generated heat, and faces the diseases such as fever at night, is often equipped with infusion treatment with Western medicine, but this kind of side effects of pharmaceutical drugs are greatly, especially concerning children's, and not easily life-time service.Therefore, study a side effect little, the medicine effectively treating fever of children infantile convulsion has realistic meaning.

Summary of the invention

Technical problem to be solved by this invention is that providing a kind of treats medicine of fever of children infantile convulsion and preparation method thereof.Described medicine has heat-clearing and toxic substances removing, effect of expelling pathogenic factors from the exterior eliminating phlegm.For acute infantile convulsion, typhoid fever is generated heat, and faces fever at night, urine band blood, dimly visible not the going out of measles and cause fever of the body to cough; Bloody dysentery, watery diarrhea, food stagnation, stomachache.Evident in efficacy, side effect is little.

For solving the problems of the technologies described above, the present invention realizes by the following technical solutions:

A kind of medicine for the treatment of fever of children infantile convulsion, according to listed as parts by weight, primarily of Rhizoma Coptidis 5-85 part, Pulvis Cornus Bubali Concentratus 10-120 part, Cornu Naemorhedi 5-85 part, calamitas urinae hominis's (forging) 5-85 part, Semen Sojae Preparatum 5-85 part, Folium Isatidis 10-120 part, Herba Schizonepetae 10-120 part, Rhizoma Et Radix Notopterygii 10-120 part, Radix Puerariae 10-120 part, Radix Rehmanniae 10-120 part, Caulis Clematidis Armandii 10-120 part, Radix Paeoniae Rubra 10-120 part, Radix Scutellariae 10-120 part, Radix Peucedani 20-150 part, Radix Scrophulariae 20-150 part, Radix Platycodonis 20-150 part, Radix Bupleuri 5-90 part, Cacumen Tamaricis 5-90 part, Rhizoma Cimicifugae 5-60 part, Fructus Arctii (stir-fry) 20-150 part and adjuvant are prepared from.

The medicine of aforementioned therapies fever of children infantile convulsion, according to listed as parts by weight, primarily of Rhizoma Coptidis 10-40 part, Pulvis Cornus Bubali Concentratus 20-80 part, Cornu Naemorhedi 10-40 part, calamitas urinae hominis's (forging) 10-40 part, Semen Sojae Preparatum 10-40 part, Folium Isatidis 20-80 part, Herba Schizonepetae 20-80 part, Rhizoma Et Radix Notopterygii 20-80 part, Radix Puerariae 20-80 part, Radix Rehmanniae 20-80 part, Caulis Clematidis Armandii 20-80 part, Radix Paeoniae Rubra 20-80 part, Radix Scutellariae 20-80 part, Radix Peucedani 40-110 part, Radix Scrophulariae 40-110 part, Radix Platycodonis 40-110 part, Radix Bupleuri 20-60 part, Cacumen Tamaricis 20-60 part, Rhizoma Cimicifugae 10-30 part, Fructus Arctii (stir-fry) 40-110 part and adjuvant are prepared from.

The medicine of aforementioned therapies fever of children infantile convulsion, according to listed as parts by weight, be prepared from primarily of Rhizoma Coptidis 25 parts, Pulvis Cornus Bubali Concentratus 50 parts, Cornu Naemorhedi 25 parts, calamitas urinae hominis's (forging) 25 parts, Semen Sojae Preparatum 25 parts, Folium Isatidis 50 parts, Herba Schizonepetae 50 parts, Rhizoma Et Radix Notopterygii 50 parts, Radix Puerariae 50 parts, Radix Rehmanniae 50 parts, Caulis Clematidis Armandii 50 parts, Radix Paeoniae Rubra 50 parts, Radix Scutellariae 50 parts, Radix Peucedani 75 parts, Radix Scrophulariae 75 parts, Radix Platycodonis 75 parts, Radix Bupleuri 37.5 parts, Cacumen Tamaricis 37.5 parts, Rhizoma Cimicifugae 20 parts, Fructus Arctii (stir-fry) 75 parts.

A preparation method for the medicine of aforementioned therapies fever of children infantile convulsion, gets said medicine, conventionally extracts, combine with conventional pharmaceutical adjuvants, also can not add adjuvant, make traditional drug formulations.

In the preparation method of the medicine of aforementioned therapies fever of children infantile convulsion, described pharmaceutical preparation is oral formulations.

In the preparation method of the medicine of aforementioned therapies fever of children infantile convulsion, described oral formulations comprises pill, granule, tablet or capsule.

In the preparation method of the medicine of aforementioned therapies fever of children infantile convulsion, described pill is prepared like this: get Cornu Naemorhedi and be ground into fine powder and Pulvis Cornus Bubali Concentratus mixes, for subsequent use; All the other medical materials are respectively got 2/3 amount and are ground into fine powder, sieve, and fine powder is for subsequent use; Coarse powder carries cream with residue medical material decocting in water, and cream and above-mentioned powder mix general ball, and dry, coating, to obtain final product.

In the preparation method of the medicine of aforementioned therapies fever of children infantile convulsion, described granule is prepared like this: get Cornu Naemorhedi and be ground into fine powder and Pulvis Cornus Bubali Concentratus mixes, obtain mixed powder, for subsequent use; All the other medical material decocting in water carry cream, the cane sugar powder of cream and above-mentioned mixed powder and extractum amount 20% and the dextrin mixing granulation of 20%, dry, to obtain final product.

In the preparation method of the medicine of aforementioned therapies fever of children infantile convulsion, described tablet is prepared like this: get Cornu Naemorhedi and be ground into fine powder and Pulvis Cornus Bubali Concentratus mixes, obtain mixed powder, for subsequent use; All the other medical material decocting in water carry cream, and the starch of cream and above-mentioned mixed powder and extractum amount 20% mixes, tabletting, and film coating, to obtain final product.

In the preparation method of the medicine of aforementioned therapies fever of children infantile convulsion, described capsule is prepared like this: get Cornu Naemorhedi and be ground into fine powder and Pulvis Cornus Bubali Concentratus mixes, obtain mixed powder, for subsequent use; All the other medical materials with 70% alcohol reflux, extracting solution reclaims ethanol and is also condensed into extractum, and the starch of extractum and above-mentioned mixed powder and extractum amount 20% and the magnesium stearate of 0.6% mixs, and with the alcohol granulation of 60%, drying, filling, to obtain final product.

Medicine of the present invention is prepared from primarily of Rhizoma Coptidis, Pulvis Cornus Bubali Concentratus, Cornu Naemorhedi, calamitas urinae hominis, Semen Sojae Preparatum, Folium Isatidis, Herba Schizonepetae, Rhizoma Et Radix Notopterygii, Radix Puerariae, Radix Rehmanniae, Caulis Clematidis Armandii, Radix Paeoniae Rubra, Radix Scutellariae, Radix Peucedani, Radix Scrophulariae, Radix Platycodonis, Radix Bupleuri, Cacumen Tamaricis, Rhizoma Cimicifugae and Fructus Arctii.Wherein, Rhizoma Coptidis energy heat clearing and damp drying, eliminating fire and detoxication.Rhizoma Coptidis also has antibacterial, antifungal, antiviral, anti-ameba, antiinflammatory, diarrhea, refrigeration function, blood sugar lowering, blood fat reducing, antioxidation, antiulcer action.For damp and hot feeling of fullness, vomiting acid regurgitation, dysentery, jaundice, unconsciousness due to high fever, hyperactivity of heart-fire, dysphoria and insomnia, heat in blood tells nosebleed, conjunctival congestion, and toothache, quenches one's thirst, carbuncle furuncle; External treatment eczema, eczema, auditory meatus is suppurated.It is burning hot that Rhizoma Coptidis (processed with wine) is apt to the clear part of the body cavity above the diaphragm housing the heart and lungs.Also for conjunctival congestion, aphtha.Prepared RHIZOMA COPTIDIS with rhizoma zingiberis recens juice clearing stomach stomach function regulating preventing or arresting vomiting.Also tie mutually for cold and heat, damp and hot middle resistance, feeling of fullness is vomitted.And cornel Rhizoma Coptidis soothing liver-QI stomach function regulating preventing or arresting vomiting.For incoordination between the liver and stomach, vomiting acid regurgitation.Pulvis Cornus Bubali Concentratus is applicable to prurigo, epilepsy, and the symptoms such as hyperpyrexia convulsion mainly contain heart tonifying, and antiinflammatory, infection are calm, relieving convulsion, shortens the bleeding time, reduces capillary permeability, the effects such as excited Pituitary Adrenalcortical system.Ether or 95% ethanol extraction of Cornu Bubali all have sedation to rat.Cornu Bubali decoct has the effect obviously shortening the bleeding time, and its LVFS is 43.24%.To go calcium sheep blood plasma to test, Cornu Bubali, except calcic, finds no the existence promoting blood coagulation substance.Successive administration 2-4 week can reduce capillary permeability.These effects can illustrate that Cornu Bubali also has effect of cooling blood and dissolving purpura.Cornu Naemorhedi, is cattle and sheep section horn, is a kind of medicine.Calamitas urinae hominis is thin slice or the block sheet of the canescence amorphism condensed in wooden pail for urine or urine cylinder, and clean dry forms.For laryngopharynx swelling and pain, or the disease such as ulcerative gingivitis aphtha, the medicines such as Rhizoma Coptidis, Cortex Phellodendri, catechu, Indigo Naturalis, Borneolum Syntheticum, Borax can be coordinated, grind external application into powder.To diseases such as the spitting of blood caused by heat in blood, epistaxis, the blood-cooling hemostatics such as Cacumen Platycladi, Radix Cirsii Japonici, Herba Cirsii, charred Nodus Nelumbinis Rhizomatis can be coordinated with using." detailed outline " is said: calamitas urinae hominis, falls fire mutually, repercussive blood, covers saltyly to walk blood therefore also by relaxing the bowels with lubricant drugs.The all skin ulcers of the sick dispute of moderns, with effective, testing also of pathogenic fire reducing.... pathogenic fire reducing repercussive blood, control the raw skin ulcer of throat ability to speak, infantile malnutrition NI, bleeding from all orifices, skin hematohidrosis." Bencao Jingshu " is said: drown white Yin, its salty in the mouth, and gas is cool, nontoxic, the fire that energy eliminating pathogen in the liver, kidney, three Jiao, bladder are had a surplus.The pungent loose hardship of Semen Sojae Preparatum is let out cold in nature, enters lung meridian, and have and evacuate the saturating property of a surname, can fall apart pathogenic factor in the exterior thoroughly, and fall apart hot and suffocating to declare again, the power of diaphoresis rather steadily, has diaphoresis not the saying of impairment of YIN." Mingyi Bielu " is said: " main cold headache cold and heat, miasma is vicious." conventional control diseases caused by exogenous pathogenic factor from the beginning of, disease sees fever with aversion to cold, lossless, card such as headache nasal obstruction grade." property of medicine opinion " carries: " controlling dysentery stomachache ".And " Fan Wangfang " fermented soya beans, salted or other wise Chinese onion soup, then cure the wound with this crystalline substance and tremble with fear cruelly and stagnant dysentery stomachache.Therefore this product has effect of clearing away heat to cure dysentery.If disease sees that times of defecation increases and measures few, stomachache, tenesmus, lower mucus and sanguinopurulent stool.This is the outer gas by epidemic disease caused by damp-heat pathogen poison, and internal injury diet raw food, stagnates caused by intestinal.Available this product clearing away heat to cure dysentery.Folium Isatidis is leaf or the branch and leaf of Verbenaceae Herba Diclipterae Chinensis, few section plant polygonum tinctorium ait., cruciferae isatis, careless Folium Isatidis or acanthaceous vegetable acanthaceous indigo etc.Main product Jiangsu, Anhui, Hebei, Henan, zhejiang and other places.Nature and flavor bitter cold.Function: heat-clearing and toxic substances removing, cooling blood for hemostasis, speckle removing.Main fever caused by exogenous pathogenic factors intenseness of heat excessive thirst, laryngopharynx swelling and pain, aphtha, jaundice, pyrotoxic dysentery, acute enteritis, swollen ulcer drug, epistaxis, stranguria with blood, traumatic hemorrhage.Herba Schizonepetae is labiate, enters medicinal its dry stem and leaf and spica.Fresh and tender bud children's is calm best, balm leaf yellow green, stem side shaped microstrip purple, cross section yellow-white, the slightly black purple yellow green of tassel.Taste is put down, and warm in nature, nontoxic, faint scent is dense.Herba Schizonepetae is diaphoresis, antipyretic, is one of conventional medical herbs of China.Can town expectorant, timid wind, removing heat from blood.Control influenza, the diaphoresis of headache cold and heat, vomiting.Rhizoma Et Radix Notopterygii, warm in nature, dispelling cold and dampness, for affection of exogenous wind-cold, have a headache lossless, cold-damp numbness, upper limb rheumatalgia.Can relieving the exterior syndrome by diaphoresis, again can wind-damp dispelling and pain relieving, but when as diaphoretic for the treatment of colds and influenza, its wind-expelling pain-stopping effect should be combined closely, time namely clinically for wind and cold table disease, the diseases such as headache or arthralgia must be had concurrently, just consider use.As for using dispelling the wind and dampness pathogens arthralgia pain, no matter with or without table disease, all can apply.According to clinical practical experiences, these product effect of bringing down a fever is fine, and antipyretic such as the product such as Herba Taraxaci, Radix Isatidis can be coordinated to control wind heat table disease, and general after heat is moved back without fever phenomenon once again.Radix Puerariae is the health product that people commonly use, and has expelling pathogenic factors from muscles for reducing heat, promoting the production of body fluid to quench thirst, rash, yang invigorating antidiarrheal, the effect of dredge the meridian passage.People Chang Zuowei expelling summer-heat, the beverage relieved the effect of alcohol.Also can be used for fever caused by exogenous pathogens headache, stiff nape and back, thirsty, quench one's thirst, measles without adequate eruption, hematodiarrhoea, has loose bowels, dizziness and headache, apoplectic hemiplegia, obstruction of qi in the chest and cardialgia, damage of the spleen and stomach caused by alcoholism.Pharmacological research shows, Radix Puerariae reduces blood pressure, decreased heart rate, reduction myocardial oxygen consumption, expansion coronary vasodilator, improve metabolism that is normal and ischemic myocardium, improve cerebral circulation, peripheral vessels and microcirculation, arrhythmia, reducing blood sugar and blood fat, antioxidation, antitumor, Platelet always, improves the pharmacological action such as immunity and memory.Radix Rehmanniae has clearing away heat and cooling blood effect and to join the army blood for warm febrile disease, high fever coma, xerostomia crimson tongue.As qingying decoction.Control the convalescent stage of febrile disease, waste heat not to the greatest extent, hinder, night fever abating at dawn, and red tongue rapid pulse person, as QINGHAOBIEJIATANG by cloudy liquid.Can be used for controlling warm febrile disease to join the army blood, heat in blood poison is contained, and hematemesis and epistaxis, macule purple is black.Caulis Clematidis Armandii has inducing diuresis for treating stranguria syndrome, and clear away heart-fire relieving restlessness, stimulating milk secretion of stimulating the menstrual flow, and for stranguria, edema, vexed dark coloured urine, aphtha of the mouth and tongue, amenorrhea breast is few, damp and hot arthralgia pain.Radix Paeoniae Rubra is famous wild genuine Chinese crude drug, has clearing away heat and cooling blood, effect of eliminating stasis to stop pain.For thermal man's nutrient blood, maculae caused by violent heat pathogen, hematemesis and epistaxis, conjunctival congestion and swelling pain, hypochondriac pain due to stagnation of liverQI, amenorrhea dysmenorrhea, addiction abdominal mass is suffered from abdominal pain, injury from falling down, carbuncle skin infection.Radix Scutellariae bitter in the mouth, cold in nature, there are heat clearing and damp drying, eliminating fire and detoxication, hemostasis, the effect such as antiabortive.Cure mainly the diseases such as epidemic febrile disease, upper respiratory tract infection, cough due to lung-heat, damp and hot jaundice, pneumonia, dysentery, hemoptysis, conjunctival congestion, frequent fetal movement, hypertension, carbuncle furuncle.Radix Peucedani hyoscine is conventional Chinese medicine.Can antipyretic, eliminate the phlegm, cure cold cough, bronchitis and furuncle and phyma.Cure mainly dispelling wind-heat, the therapeutic method to keep the adverse QI flowing downwards, expectorant.Control headache due to pathogenic wind-heat, expectorant heat syndrome cough is breathed heavily, vomiting, and chest and diaphragm is full vexed.Radix Scrophulariae has clearing away heat and cooling blood; Nourishing YIN to lower pathogenic fire; Effect of detoxicating and resolving stagnation of pathogens.Main warm febrile disease and nutrient blood; Fever of the body; Excessive thirst; Crimson tongue, send out speckle, hectic fever due to YIN-deficiency chronic cough, fidgets due to deficiency is awake not, and constipation is hindered in Tianjin, and order is puckery dim-sighted, and throat larynx swells and ache, scrofula sucutaneous nodule, skin sore.Radix Platycodonis is campanulaceae Platycodon grandiflouorum plant, grows at China, the Korea peninsula, Japan and eastern Siberia.Root can be used as medicine, and also can pickle into brined vegetable, is called " Calculus Canitis " brined vegetable in Northeast Area of China.There is lung qi dispersing, eliminate the phlegm, sore-throat relieving, evacuation of pus, sharp the five internal organs, effect of filling blood, mending five kinds of over strain, foster the spirit of nobility.Effectively can treat cough with copious phlegm, laryngopharynx swelling and pain, lung abscess vomiting pus, fullness in the chest and hypochondriac pain, dysentery stomachache, aphtha of the mouth and tongue, conjunctival congestion and swelling pain, uroschesis.The medical herbs that Radix Bupleuri is included for " Chinese Pharmacopoeia ", medicinal part is the dry root of umbelliferae bupleurum or Radix Bupeuri Scorzonerfolii..There is reconciling superficies and interior, effect of soothing the liver yang invigorating.For cold, fever, alternate attack of chill and fever, malaria, stagnation of QI due to depression of the liver, sternal rib pain, proctoptosis, uterine prolapse, menoxenia.Cacumen Tamaricis twig and Ye Ke are used as medicine, the flat sweet-salty of property, can saturating sun stroke rash.Major function: deliver rash, removing toxic substances, diuresis, wind-damp dispelling.Rhizoma Cimicifugae feeble QI, mildly bitter flavor and puckery.Rhizoma Cimicifugae one medicine, mainly contains elevate a turnable ladder and thoroughly sends out, heat-clearing and toxic substances removing and deliver the effects such as rash.Can effectively treat seasonal epidemic pathogens epidemic disease, headache cold and heat, laryngalgia, aphtha, macule is not saturating; Sinking of QI of middle-JIAO, chronic diarrhea chronic dysentery, proctoptosis, women is collapsed, band, and uterus drops; Carbuncle sore tumefacting virus.Fructus Arctii has dispelling wind and heat pathogens, lung qi dispersing rash, sore-throat relieving eliminating stagnation, effect of removing toxic substances and promoting subsidence of swelling.Belong to wind-heat-dispersing medicinal in diaphoretic medicine.Modern study, Fructus Arctii also can be used for preventing and treating diabetic nephropathy; The arctigenin that Arctium lappa fruit generates through hydrolysis containing arctigenin has active anticancer.

Said medicine prescription, plays heat-clearing and toxic substances removing altogether, effect of expelling pathogenic factors from the exterior eliminating phlegm.For acute infantile convulsion, typhoid fever is generated heat, and faces fever at night, urine band blood, dimly visible not the going out of measles and cause fever of the body to cough; Bloody dysentery, watery diarrhea, food stagnation, stomachache.Evident in efficacy, side effect is little.

Applicant carried out following experiments, provable the present invention has effective effect.

experimental example 1 pharmacodynamics test

1 experiment material

1.1 medicine

Medicine of the present invention (being prepared by the method for embodiment 1), positive drug :child rejuvenates ball, is provided, lot number: 20140116 by Dechangxiang Medical Industry Co Ltd, Guiyang.

Usage and dosage: warm water is ground clothes or taken, below one full year of life each 1,1-2 year each 2,3-4 year each 3,5-7 year each 5,2-3 time on the one.Specification: every ball weighs 0.18 gram.

Dosage arrange: oral, the dose 0.36-0.54g of day below one full year of life, 1-2 year day dose 0.72-1.08g, 3-4 year day dose 1.08-1.62g, 5-7 year day dose 1.8-2.7g.

This research with 1-3 year, day dose 1.08g count, body weight by 3 × 2+8=14kg calculating, namely the daily dosage of child is 0.077g/kg.

Mouse Weight 12-14g, rat body weight 40-60g.Effect experiment pill, dosage all heavily represents with ball herein.

Mice high dose gavage 1.54g/kg(is equivalent to 20 times of clinical people's consumption)

Middle dosage gavage 0.77g/kg(is equivalent to 10 times of clinical people's consumption)

Low dosage gavage 0.38g/kg(is equivalent to 5 times of clinical people's consumption)

Rat high dose gavage 1.078g/kg(is equivalent to 14 times of clinical people's consumption)

Middle dosage gavage 0.539g/kg(is equivalent to 7 times of clinical people's consumption)

Low dosage gavage 0.270g/kg(is equivalent to 3.5 times of clinical people's consumption)

1.2 animal

Kunming mouse, male and female dual-purpose, body weight 12-14g, SD rat, male and female dual-purpose, body weight 40-60g, by Chongqing Institute of Chinese Medicine institute of lab animals (credit number: SCXK(changes) 2012-0006) and Military Medical Univ No.3, P.L.A's Experimental Animal Center (credit number: SCXK-(army) 2012-0003) provide.

1.3 reagent

Glacial acetic acid, Chengdu Ke Long chemical reagent factory, lot number: 20120803; Dimethylbenzene, Tianjin great Mao chemical reagent factory, lot number: 20080921; Chloral hydrate, Beijing chemical reagents corporation, lot number: 20100218; Nikethamide Injection, Shanghai He Feng pharmacy, lot number: 1308061; Yeast tablet, Yantai City Shen En Food Co., Ltd, lot number: 20140223; Ammonia, Beijing Chemical Plant, lot number: 20120814; Phenol red, Tianjin Jin Bei Fine Chemical Co., Ltd, lot number: 20121107; Carrageenan, Henan Tian Xing food additive company limited, lot number: 20110923.

1.4 zoopery places

Guiyang College of Traditional Chinese Medicine's pharmacological toxicology laboratory, well-ventilated, sanitation and hygiene, temperature 20-25 DEG C, humidity 55%-65%.The other sub-cage rearing of animal unisexuality, feed of freely drinking water.

1.5 key instrument

JM-B type electronic balance, the inscription of Yuyao City discipline is weighed calibration equipment company limited; ALC-210.3 type electronic balance, upper current chart level instruments and meters company limited; Table model high speed centrifuge, Anting Scientific Instrument Factory, Shanghai; 721 spectrophotometers, Shanghai precision instrumentation company limited; Nebulizer, Yuyue Medical Apparatus Co., Ltd., Jiangsu; ZZ-6 type mice autonomic activities instrument, Chengdu TME Technology Co., Ltd.; RB-200 intelligence hot-plate instrument, Shanghai benefit connection science and education equipment company limited.

1.6 statistical procedures

Data with ± Srepresent, adopt SPSS 17.0 statistical analysis software to carry out statistical procedures, measurement data and compare application one factor analysis of variance between two between many groups, P<0.05 has statistical significance.

2 experimental techniques and result

The experimentation of 2.1 cough-suppressing phlegm-dispelling functions

2.1.1 ammonia is drawn to the impact coughing mice

Kunming mouse 70, male and female half and half, body weight 12-14g, is divided into 5 groups at random, i.e. blank group, the high, medium and low dosage group of the present invention, and positive drug child rejuvenates ball group, often organizes 14, gastric infusion, 0.2ml/10g, once a day, successive administration 5 times.

The laggard whereabouts of last administration 1h cough experiment.Specification is selected to be that the bottomless plastic casing of 22cm × 16cm × 10cm is as atomized cover, in nebulizer, 2ml ammonia (needing at every turn to change) atomization 30s is first added during experiment, the ammonia of atomization is made to be full of atomized cover, mice is put into atomized cover, and observed and recorded is from putting into 5min mouse cough incubation period and cough number of times atomized cover respectively.The results are shown in Table 1.

Note: each administration is compared with blank group, * p<0.05 * * p<0.01.

As seen from Table 1, compare with blank group, each administration group all can extend the mouse cough incubation period that ammonia brings out significantly, reduce cough number of times (p<0.05, p<0.01), show the coughre flex of the mice that medicine of the present invention can suppress ammonia to bring out, alleviate cough symptom, have obvious antitussive action, its effect is suitable with child's ball of rejuvenating.

2.1.2 on the impact of mice trachea section phenols contents

Kunming mouse 70, male and female half and half, body weight 12-14g, is divided into 5 groups at random, i.e. blank group, the high, medium and low dosage group of medicine of the present invention, and positive drug child rejuvenates ball group, often organizes 14, gastric infusion, 0.2ml/10g, once a day, successive administration 5 times.

Namely every mouse carotid dorsal sc injection 0.6% phenol red solution is engraved on, 0.2ml/10g after last administration.After 1h, mice is taken off cervical vertebra to put to death, after back of the body position is fixing, cut skin along cervical region median line, expose trachea, ligation trachea under cricoid cartilage, take out trachea and lung tissue, in normal saline, clean bloodstain be completely placed on 3ml 5% NaHCO ₃in solution, shred trachea and lung tissue, soak the centrifugal 20min of 30min, 2000r/min, get supernatant 560nm place colorimetric, calculate phenol red content in flushing liquor.

The drafting of phenol red standard curve:

Take phenol red 0.1g by analytical balance standard and be dissolved in 5% NaHCO ₃to 100ml in solution, be diluted to every milliliter in turn containing phenol red 6 μ g, 5 μ g, 4 μ g, 3 μ g, 2 μ g, 1 μ g.Mensuration wavelength is 560nm, by spectrophotometer measurement OD value.With phenol red content for vertical coordinate, OD value is abscissa, obtains phenol red standard curve and regression equation.

Surveyed each sample OD value is substituted into equation, obtains the concentration that each sample is phenol red, compare between organizing.The results are shown in Table 2.

Note: each administration is compared with blank group, * p<0.05 * * p<0.01.、

As seen from Table 2, compare with blank group, the rejuvenate trachea section phenols contents of ball group mice of medicine ball high dose group of the present invention and child is all significantly increased (p<0.05, p<0.01), show that medicine of the present invention can promote to have obvious phlegm-dispelling functions by the excretion that trachea is phenol red.

The experimentation of 2.2 antipyretic anticonvulsant actions

2.2.1 to the refrigeration function of yeast pyrogenicity rat

SD rat 60, male and female half and half, body weight 40-60g, is divided into 5 groups at random, i.e. blank group, the high, medium and low dosage group of medicine of the present invention, and positive drug child rejuvenates ball group, often organizes 12, gastric infusion, 1ml/100g, once a day, successive administration 5 times.After last administration, the yeast suspension 0.6ml/100g of dorsal sc injection 15% immediately, measure and after recording injection yeast suspension 0,4,6, the rat anus temperature of 8h.By injection yeast suspension after 0h measure anus temperature be designated as basic anus temperature, injection yeast suspension after 4,6,8h mensuration anus temperature deduct the temperature approach that basic anus temperature is different time points respectively, carry out statistical procedures.The results are shown in Table 3.

Note: each administration group compares with blank group, * p<0.05 * * p<0.01.

As seen from Table 3, compare with blank group, each administration group different time points all can reduce rat anus temperature temperature approach, high, the middle dosage group of medicine of the present invention 6,8h cooling the most obviously (p<0.05), medicine low dose group of the present invention 4,8h cooling the most obviously (p<0.05, p<0.01), show that medicine of the present invention has certain refrigeration function.

2.2.2 the effect of heat resistance convulsions

SD rat 70, male and female half and half, body weight 40-60g, is divided into 5 groups at random, i.e. blank group, the high, medium and low dosage group of medicine of the present invention, and positive drug child rejuvenates ball group, often organizes 14, gastric infusion, 1ml/100g, once a day, successive administration 5 times.

Rat anus temperature is measured after last administration 1h, bring out febrile convulsion outbreak subsequently (rat to be placed in 46 ± 0.5 DEG C of hot baths and to bring out febrile convulsion outbreak, only expose head when the depth of water is stood along beaker with rat to be as the criterion, can wear rubber gloves when every rat enters water is pressed in water, treat that its whole body fur drenches, can loose one's grip when beaker is stood, faint from fear after occurring and depart from water-bath immediately.With rat body, tetanic or generalized tonic-clonic is convulsive attack in this experiment.）。Observe each group of rat faint from fear incubation period (from bringing out the time of fainting from fear to convulsive attack, the longest observations 5min, 5min be now convulsive attack not, counts 5min), duration of status convulsion, bring out anus temperature when terminating rear 2min.The anus temperature rate of climb=(during anus temperature when bringing out 2min after terminating-bring out beginning anus temperature) ÷ faints from fear incubation period.The results are shown in Table 4.

Note: each administration group compares with blank group, * p<0.05 * * p<0.01.

As seen from Table 4, compare with blank group, each administration group all can be fainted from fear incubation period by significant prolongation rat, shorten duration of status convulsion, the anus temperature that the slows down rate of climb (p<0.05, p<0.01), show that medicine of the present invention has certain antipyretic anticonvulsant action.

2.2.3 anti-nikethamide causes the effect of mice convulsion

Kunming mouse 70, male and female half and half, body weight 12-14g, is divided into 5 groups at random, i.e. blank group, the high, medium and low dosage group of the present invention, and positive drug child rejuvenates ball group, often organizes 14, gastric infusion, 0.2ml/10g, once a day, successive administration 5 times.Last administration 1h, each treated animal equal subcutaneous injection nikethamide 0.75g/kg, observed and recorded respectively organizes surviving animals number in mice convulsion incubation period and 24h immediately.The results are shown in Table 5.

Note: each administration group compares with blank group, * p<0.05.

As seen from Table 5, compare with blank group, each administration group all can extend mice convulsion incubation period, wherein with medicine high dose group of the present invention effect significantly (p<0.05), and the survival rate of mice is up to 64.3%, show that medicine of the present invention can resist the convulsions caused by central stimulants nikethamide, there is obvious anticonvulsant action.

The experimentation of 2.3 anti-diarrhea effects

2.3.1 on the impact of mouse small intestine ahead running

Kunming mouse 70, male and female half and half, body weight 12-14g, is divided into 5 groups at random, i.e. blank group, the high, medium and low dosage group of medicine of the present invention, and positive drug child rejuvenates ball group, often organizes 14, gastric infusion, 0.2ml/10g, once a day, successive administration 5 times.

Small intestine advance activity experiment is carried out after last administration 1h.Every mice equal gavage 10% charcoal end normal saline 0.6ml, after 20min, de-cervical vertebra puts to death mice, cut open the belly and get stomach-small intestinal-caecum, overall taking-up, not adding traction is laid on smooth blank, measures respectively from sphincter of pylorus to front end, charcoal end (charcoal end advance distance) and the distance to caecum front end (small intestinal length).Calculate Intestinal propulsive rate.The results are shown in Table 6.

Intestinal propulsive rate (%)=charcoal end advance distance ÷ small intestinal length × 100%

Note: each administration is compared with blank group, * p<0.05 * * p<0.01.

As seen from Table 6, compare with blank group, each administration group all can reduce mouse small intestine propelling rate (p<0.05 significantly, p<0.01), show that medicine of the present invention can slow down the propelling of mouse small intestine, have the effect of certain suppression gastrointestinal motility, its effect is suitable with child's ball of rejuvenating.

2.3.2 on the impact of Radix Et Rhizoma Rhei induced mice diarrhoea

Kunming mouse 84, male and female half and half, body weight 12-14g, is divided into 6 groups at random, i.e. blank group, model group, the high, medium and low dosage group of medicine of the present invention, positive drug child rejuvenates ball group, often organizes 14, gastric infusion, 0.2ml/10g, once a day, successive administration 5 times.

After last administration 1h, except blank group, all the other are respectively organized mice and (get rhubarb powder 100g with 100% Radix Et Rhizoma Rhei charcoal end suspension respectively, add 10% charcoal end normal saline submergence, filter after merceration 24h, 40 DEG C of water-baths are condensed into 1g/ml, cryopreservation, used time water-bath is heated to 25 DEG C) 0.6ml gavage, blank group gavage 10% charcoal end normal saline 0.6ml.With charcoal end for indicator, record mice arranges time of melena, character, number of times, the defecation situation of Continuous Observation 6h first, calculates stool in mice character integration.(stool in mice character ranking criterion: 0 point, feces is granular, black, matter are hard, flexible; 1 point, feces is granular, color is pale brown, matter is soft; 2 points, feces pasty state, color are pale brown; 3 points, feces water sample, yellow skin.) routine gives mice drinking-water and feedstuff, the accuracy of guarantee test result in experimentation.The results are shown in Table 7.

Note: each administration is compared with model group, * p<0.05 * * p<0.01.

As seen from Table 7, can mice be caused rapidly to suffer from diarrhoea with Radix Et Rhizoma Rhei infusion gavage mice, feces be water sample, diarrhoea number of times showed increased.Each administration group all can extend the time of mice defecation first significantly, reduce diarrhoea number of times, alleviating diarrhoea degree, feces is made to be tending towards pasty state or soft grit shape (p<0.05, p<0.01), show the diarrhea of mouse that medicine of the present invention can suppress Radix Et Rhizoma Rhei infusion gavage and causes, reduce diarrhoea number of times and diarrhoea degree, have obvious anti-diarrhea effect, its effect is suitable with child's ball of rejuvenating.

2.4 sedation and analgesia antiinflammatory experimentatioies

2.4.1 on the impact of mice autonomic activities

Kunming mouse 70, male and female half and half, body weight 12-14g, is divided into 5 groups at random, i.e. blank group, the high, medium and low dosage group of medicine of the present invention, and positive drug child rejuvenates ball group, often organizes 14, gastric infusion, 0.2ml/10g, once a day, successive administration 5 times.Last administration 1h, mice is put into ZZ-6 type mice autonomic activities instrument, conform 2min, then records mice autonomic activities number of times in 5min continuously.The results are shown in Table 8.

Note: each administration group compares with blank group, * p<0.05.

As seen from Table 8, compare with blank group, each administration group all can reduce creep number of times and the number of times of standing of mice, wherein with high dose group effect of the present invention significantly (p<0.05), show that medicine of the present invention can reduce the autonomic activities of mice, there is certain sedation.

2.4.2 thermostimulation is caused to the impact (hot plate method) of mice pain

Screen qualified female mice: hot-plate instrument temperature is 55 ± 0.5 DEG C, removed by the mice that pain threshold (mice to lick the time " second " of metapedes at hot plate) is less than 5 seconds and is greater than 30 seconds, all the other qualified mices (pain threshold 5-30 second between) are included next step in and formally test.Select 70 qualified mices, body weight 12-14g, is divided into 5 groups at random, i.e. blank group, the high, medium and low dosage group of medicine of the present invention, and positive drug child rejuvenates ball group, often organizes 14, gastric infusion, 0.2ml/10g, once a day, and successive administration 5 times.After last administration, 30min, 90min, 150min measure the pain threshold of each treated animal respectively, observe medicine to the impact of animal pain threshold.The results are shown in Table 9.

Note: each administration group compares with blank group, * p<0.05.

As seen from Table 9, the pain threshold of each treated animal there are no significant difference before medicine, except child's ball positive drug of rejuvenating significantly extends except the effect of mice pain threshold showing during 30min after medicine after medicine, all the other each administration groups are showed no the effect significantly increasing mice pain threshold, show that of the present invention group causes mice pain without obvious inhibitory action to thermostimulation.

2.4.3 Dichlorodiphenyl Acetate causes the impact (writhing method) of mouse writhing reaction

Kunming mouse 70, male and female half and half, body weight 12-14g, is divided into 5 groups at random, i.e. blank group, the high, medium and low dosage group of medicine of the present invention, and positive drug child rejuvenates ball group, often organizes 14, gastric infusion, 0.2ml/10g, once a day, successive administration 5 times.Last administration 1h, each Mus equal lumbar injection 0.6% acetum 0.2ml/ only, observe immediately and record writhing response number of times (secondary) in animal 15min, and calculating writhing inhibition percentage.The results are shown in Table 10.

Suppression ratio (%)=(blank group average writhing number-administration group average writhing number) average writhing number × 100% of ÷ blank group

Note: each administration group compares with blank group, * p<0.05.

As seen from Table 10, compare with blank group, each administration group all can reduce acetic acid induced mice writhing response number of times (p<0.05) significantly, shows that medicine of the present invention has certain analgesic activity, and its effect is suitable with child's ball of rejuvenating.

2.4.4 xylol causes the impact of mice auricle swelling

Kunming mouse 70, male and female half and half, body weight 12-14g, is divided into 5 groups at random, i.e. blank group, the high, medium and low dosage group of medicine of the present invention, and positive drug child rejuvenates ball group, often organizes 14, gastric infusion, 0.2ml/10g, once a day, successive administration 5 times.Last administration 1h, with a dimethylbenzene stock solution 0.03ml/ uniform application on the left ear two sides of mice, after 40min, put to death animal, two ears about rapid clip, to beat in same area with the card punch of diameter 8mm and get left and right ear auricle, precise weighing, the difference weighed with left and right ear auricle, for mice ear degree, carries out statistical procedures.The results are shown in Table 11.

Suppression ratio (%)=(blank group ear swelling degree-administration group ear swelling degree) ÷ blank group ear swelling degree × 100%

As seen from Table 11, compare with blank group, each administration group all significantly can alleviate dimethylbenzene induced mice auricle swelling degree (p<0.05, p<0.01), wherein obvious with medicine high dose group of the present invention effect, its inhibitory rate of intumesce is 44.2%, shows that medicine of the present invention has the acutely inflamed effect of certain suppression, can eliminate the symptoms such as acutely inflamed red and swollen heat pain rapidly.

Note: each administration group compares with blank group, * p<0.05.

2.4.5 the impact of rat paw edema caused by Carrageenan

SD rat 60, male and female half and half, body weight 40-60g, is divided into 5 groups at random, i.e. blank group, the high, medium and low dosage group of medicine of the present invention, and positive drug child rejuvenates ball group, often organizes 12, gastric infusion, 1ml/100g, once a day, successive administration 5 times.

Last administration 1h, at Rat Right rear foot foot sole of the foot portion intradermal injection 1% carrageenin 0.1ml, after Yu Zhiyan 1,2,4h vernier caliper measurement foot sole of the foot thickness, and measure the thickness of left foot corresponding site, calculate swelling rate.The results are shown in Table 12.

Swelling rate (%)=(right crus of diaphragm foot sole of the foot thickness-left foot foot sole of the foot thickness)/left foot foot sole of the foot thickness × 100%

Note: each administration group compares with blank group, * p<0.05 * * p<0.01.

As seen from Table 12, compare with blank group, medicine of the present invention each dosage group all obviously can alleviate the paw swelling of rat at multiple time point, wherein with medicine high dose group of the present invention effect the most obviously (p<0.05, p<0.01), it eliminates sustainable more than the 4h of effect of acute inflammation swelling, shows that medicine of the present invention has the effect of certain anti-inflammation.

2.4.6 on the impact that rat granuloma is swollen

Get SD rat, male and female half and half, body weight 40-60g.Rat, under chloral hydrate (300mg/kg) anesthesia, in chest center work one opening, peels off skin to axillary fossa place with mosquito forceps, will implant axillary fossa place, skin suture by autoclaved 30mg cotton balls in advance, administration of dividing into groups after animal is clear-headed.

The rat of successful surgery is divided into 5 groups at random, i.e. blank group, the high, medium and low dosage group of medicine of the present invention, positive drug child rejuvenates ball group, often organizes 12, gastric infusion, 1ml/100g, once a day, and successive administration 14 times.1h after last administration, de-cervical vertebra puts to death animal, opens skin of chest, carefully peels off cotton balls and granulation tissue, is placed on electronic balance and weighs, be cotton balls granulation weight in wet base.Cotton balls granulation is placed in 60 DEG C of baking ovens, dry 24 hours, takes out weighing and be cotton balls granulation dry weight.Deducting cotton balls recast by cotton balls granulation weight in wet base, dry weight is respectively that the how many index of granulation carries out statistical procedures.The results are shown in Table 13.

Note: each administration group compares with blank group, * p<0.05 * * p<0.01.

As seen from Table 13, compare with blank group, each administration group all can suppress rat granuloma significantly, alleviate granulation weight in wet base or dry weight (p<0.05, p<0.01), wherein obvious with medicine high dose group of the present invention effect, show that medicine of the present invention can suppress granulation hyperplasia, have the effect of certain anti-chronic inflammatory disease, its effect is suitable with child's ball of rejuvenating .

By above-mentioned experimentation, result shows:

(1) medicine of the present invention has cough-suppressing phlegm-dispelling functions: can extend ammonia and draw and cough mouse cough incubation period and reduce cough number of times, and increase mice trachea phenols contents.

(2) medicine of the present invention has antipyretic anticonvulsant action: can reduce rat fever caused by yeast, and the different time points rat anus temperature difference is obviously declined; Can extend febrile convulsion rat faint from fear incubation period, shorten duration of status convulsion, and reduce the anus temperature rate of climb; Nikethamide mice convulsion incubation period can be extended and promote the survival of mice.

(3) medicine of the present invention has anti-diarrhea effect: significantly can reduce mouse small intestine propelling rate, extends the defecation time first of Radix Et Rhizoma Rhei induced mice diarrhoea, reduces diarrhoea number of times and alleviating diarrhoea degree, improves feces character.

(4) medicine of the present invention has sedation and analgesia antiinflammatory action: the autonomic activities number of times that can reduce mice; Reduce acetic acid cause mouse writhing stoichiometric number and increase the pain threshold of hot plate mice; Alleviate mice caused by dimethylbenzene xylene auricle swelling degree, suppress the swelling of carrageenin rat toes, alleviate granuloma induced by implantation of cotton pellets rat granulation weight in wet base and dry weight.

experimental example 2 long term toxicity test

1 experiment material

1.1 medicine

Medicine of the present invention (being prepared by embodiment 1), usage and dosage: warm water is ground clothes or taken, below one full year of life each 1,1-2 year each 2,3-4 year each 3,5-7 year each 5,2-3 time on the one.Specification: every ball weighs 0.18 gram.

Dosage is arranged :oral, the dose 0.36-0.54g of day below one full year of life, 1-2 year day dose 0.72-1.08g, 3-4 year day dose 1.08-1.62g, 5-7 year day dose 1.8-2.7g.

This research with 1-3 year, day dose 1.08g counts, body weight by 3 × 2+8=14kg calculate, namely clinical consumption per day is 0.077g/kg.

Face the used time is mixed with desired concn suspension oral gavage administration with distilled water.

1.2 animal

SD rat, male and female half and half, 80, body weight 80-100g, is provided by Military Medical Univ No.3, P.L.A's Experimental Animal Center, the quality certification number: SCXK-(army) 2012-0003.

1.3 key instrument

JY601 type electronic balance, upper current chart level instruments and meters company limited; OLYMPUS BH-2 microscope, Japan; SC-970 Automatic Blood Cell Analyzer, Bei Ken company of Switzerland; Hitachi 7170A automatic clinical chemistry analyzer, Kodak of the U.S.; HPIAS-1000 high-definition color picture and text pathological analysis system, light microscopic.

1.4 experimental data statistics

Experimental data with ± Srepresent, t inspection between organizing.

2 experimental techniques

2.1 experiment condition

Not every cage 5 group supports of the equal unisexuality of rat before and after administration, feed with full-valence pellet feed, freely drink water, room temperature 20 DEG C ~ 25 DEG C, humidity 55% ~ 65%.First conform before administration and raise several days, observe rat general status, change without exception, then the administration that starts to divide into groups is tested.

2.2 grouping and dosage

SD rat 80, male and female half and half, are divided into 4 groups at random, often organize 20, respectively as high, medium and low three the dosage groups of medicine of the present invention and blank group.Administration group dosage is respectively 4.620g, 2.310g, 1.155g/kg body weight, administration concentration is respectively 23.10%, 11.55%, 5.78%, every day at the upper and lower noon is respectively administered once, and is 60 times, 30 times, 15 times that said preparation drafts clinical child daily dosage 0.077g/kg body weight.Administration volume is 10ml/kg body weight.

2.3 route of administration and method

Because be oral administration, so the equal gastric infusion of each treated animal.During 8-10 in morning every day, 4-6 in afternoon time each gastric infusion once, administration volume is 10ml/kg body weight, continuous 13 weeks; Observe animal general status every day, record weekly each treated animal body weight and food-intake once, increase change according to the weight of animals, adjustment dosage.

2.4 the test period

Testing total cycle is 15 weeks, and first 13 weeks is administration time, drug withdrawal in latter 2 weeks, observes animal different time whether toxic reaction respectively.The administration phase, when administration 13 weeks, after last administration, fasting can't help water 12 hours, often group get 10 animals (male and female half and half) weigh after femoral vein get blood, measure animal blood cytology, blood biochemical analysis, put to death animal to carry out system and become celestial simultaneously, observe animal each organs and tissues with or without after hyperemia, enlargement, the ANOMALOUS VARIATIONS such as hemorrhage, get each Mus main organs again to weigh respectively, calculate after organ coefficient and other organs and tissues specimen are carefully peeled off peripheral adipose tissue etc. and immersed 10% formaldehyde and fix, carry out Pathomorphologic inspection.Withdrawal time, all the other animal drug withdrawal routines are raised 2 weeks, and every day carries out general status observation, every journal body weight and food-intake, and fasting be can't help drink and put to death animal in 12 hours, detects above-mentioned indices.

3 inspection items

3.1 overview

Observe the mode of appearance sign, behavioral activity, hair luster, feed, drinking-water, stool etc. of each treated animal every day; Find that intoxicated animals is isolated immediately, primary part observation; Find dead or moribund animals, at once postmortem.Claim the weight of animals weekly, adjust dosage accordingly, record each treated animal feed consumption simultaneously.

3.2 test item

3.2.1 blood cytology Index for examination

Leukocyte (WBC), neutrophilic granulocyte sum (#NEUT), lymphocyte absolute value (#LYMPH), monocyte count (MONO#), acidophil number (#EOS), basophilic leukocyte number (#BASO), Erythrocyte hemoglobin distribution width (RDW), neutrophilic granulocyte percentage ratio (%NEUT), cent lymphocytes (%LYMPH), mononuclear cell percentage ratio (%MONO), acidophil percentage ratio (%EOS), basophilic leukocyte percentage ratio (%BASO), erythrocyte (RBC), hemoglobin (HGB), packed cell volume (HCT), mean corpuscular volume (MCV) (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin (MCHC), platelet (PLT), Mean Platelet Volume (MPV).

3.2.2 blood biochemical analysis Index for examination

Potassium (K), sodium (Na), chlorine (CL), glucose (GLU), triglyceride (TG), T-CHOL (CHOL), blood urea nitrogen (BUN), creatinine (CREA), glutamate pyruvate transaminase (ALT), glutamic oxaloacetic transaminase, GOT (AST), alkali phosphatase (AKP), creatine kinase (CK), total protein (TP), albumin (ALB), globulin (GLB), total bilirubin (TBIL).

3.2.3 major organs tissue specimen

The heart, liver, spleen, lung, kidney, adrenal gland, thymus, brain, uterus, ovary, testis, epididymis, weigh respectively, calculates acropetal coefficient; Draw materials in same positions of organs and tissues such as the heart, liver, spleen, lung, kidney, adrenal gland, thymus, cerebral tissue, thyroid, hypophysis, trachea, esophagus, stomach, intestinal, bladder, pancreas, lymph node, spinal cord, optic nervies again and carry out paraffin embedding, section, HE dyes; Put observation by light microscope and carry out internal organs pathological tissue with blank group and compare.First comparison check high dose group and the pathological change of blank treated animal tissue morphology, middle dosage group and low dose group animal organ tissue specimen 10% formaldehyde are fixed for future reference.

3.2.4 pathological examination

Perform an autopsy on sb., and main Organ and tissue 10% formaldehyde is fixed, preserved.The histopathological examination of high dose group and blank treated animal is only carried out when the postmortem of each dosage treated animal does not find obvious pathological changes.As found, centering, low dose group animal check pathological changes accordingly again.

3.3 index observing times

The general status of all laboratory animals of complete observation every day, animal activity behavior, stool, outward appearance sign, the feedstuff consumption of a week; Weigh weekly once; Administration 13 weekend and drug withdrawal recover the end of term in 2 weeks, respectively carry out once above-mentioned project and check comprehensively.

4 experimental results

4.1 impacts on rat ordinary circumstance and feed

In successive administration 13 weeks and drug withdrawal 2 weeks, each administration group compared with blank group, observe animal activity, behavior, diet, stool, hair luster etc., 4 treated animal situations are similar, show no obvious abnormalities change.Interior rat food ration also no significant difference at one time.The results are shown in Table 14 and table 15.

Note: different time points, each administration group compares with blank group, P>0.05.

Note: different time points, each administration group compares with blank group, P>0.05.

Result shows, every animal daily consumption appetite is all in increasing trend gradually, and each administration group compares with blank group, no significant difference, P > 0.05.Show the continuous gastric infusion of rat 13 weeks and drug withdrawal 2 weeks, male and female rat chow day's expenditure is had no significant effect.

4.2 impacts on rat body weight

The high, medium and low Three doses of medicine of the present invention compares with blank group the body weight after rat successive administration different time, and its difference, without significant change, the results are shown in Table 16 and table 17.

Note: each administration group compares with blank group, P>0.05; Within 0 week, be medicine early stage, n=10; 1-13 week is the administration phase, n=10; 14-15 week withdrawal time, n=5.

Note: each administration group compares with blank group, P>0.05; Within 0 week, be medicine early stage, n=10; 1-13 week is the administration phase, n=10; 14-15 week is withdrawal time, n=5.

Result shows, in the observation period, each group male and female rat body weight increases all gradually, and growth promoter is good; Each administration group compared with same time period blank group, each time point body weight no significant difference compared with blank group, P > 0.05.Show the continuous gastric infusion of rat 13 weeks and drug withdrawal 2 weeks, male and female rat body weight is affected without overt toxicity.

4.3 affect rat blood cytology

With medicine of the present invention to rat oral gavage administration 13 weeks and drug withdrawal 2 weeks, each treated animal hematological indices testing result is in table 18, and table 19.

Note: each administration treated animal blood cytology indices numerical value respectively with blank group corresponding index numeric ratio comparatively, P>0.05.

Note: each administration treated animal blood cytology indices numerical value respectively with blank group corresponding index numeric ratio comparatively, P>0.05.

Result shows, the high, medium and low Three doses successive administration of medicine of the present invention 13 weeks and drug withdrawal 2 weeks, have no appreciable impact to the hemocyte of rat, through statistical procedures, and P > 0.05.Therefore, can think that child rejuvenates ball larger dose, long period (13 weeks) administration and drug withdrawal 2 weeks, to the hemopoietic function of rat without significant toxic effect.

4.4 on the biochemical impact of rat blood

With medicine of the present invention to rat oral gavage administration 13 weeks and drug withdrawal 2 weeks, each treated animal blood biochemical analysis Indexs measure the results are shown in Table 20 and table 21.

Note: each administration treated animal blood cytology indices numerical value respectively with blank group corresponding index numeric ratio comparatively, P>0.05.

Note: each administration treated animal blood cytology indices numerical value respectively with blank group corresponding index numeric ratio comparatively, P>0.05.

Result shows, the high, medium and low Three doses successive administration of medicine of the present invention 13 weeks and drug withdrawal 2 weeks, appreciable impact is showed no on the blood parameters of rat, every biochemical indicator numerical value such as the liver function of rat and renal function are respectively compared with blank group respective value, have no notable difference, P > 0.05; Therefore show medicine larger dose successive administration of the present invention 13 weeks and drug withdrawal 2 weeks to the index such as Liver Function, renal function all without remarkable toxic action.

4.5 impacts on rat major organs index

With medicine of the present invention to rat oral gavage administration 13 weeks and drug withdrawal 2 weeks, system obduction: the form of the internal organs such as brain, heart, liver, spleen, lungs, kidney, adrenal gland, thymus, thyroid, stomach, intestinal, testis, epididymis, prostate, uterus, ovary, size, color all do not find macroscopic change.Each treated animal main organs index testing result in table 22,23,24,25.

Note: each administration treated animal each organ index value respectively exponential quantity corresponding to blank group compares, P>0.05.

Note: each administration treated animal each organ index value respectively exponential quantity corresponding to blank group compares, P>0.05.

Note: each administration treated animal each organ index value respectively exponential quantity corresponding to blank group compares, P>0.05.

Note: each administration treated animal each organ index value respectively exponential quantity corresponding to blank group compares, P>0.05.

Result shows, the high, medium and low Three doses successive administration of medicine of the present invention 13 weeks and drug withdrawal 2 weeks, obvious toxic effect is had no to rat main organs tissue, the every organ index of each administration group rat compared with blank group all without significant difference, P > 0.05; Therefore show medicine larger dose successive administration of the present invention 13 weeks and drug withdrawal 2 weeks to each organs and tissues of rat without remarkable toxic action.

4.6 impacts on rat important organ histoorgan pathomorphism

To medicine successive administration of the present invention 13 weeks, and the drug withdrawal high, medium and low Three doses group of 2 weeks and blank group three batches, kill and get rat and solution cuts the histoorgan such as the heart, liver, spleen, lung, kidney, adrenal gland, thymus, brain, hypophysis, uterus, ovary, testis, epididymis, prostate, thyroid, trachea, esophagus, stomach, aorta, duodenum, ileum, colon, bladder, pancreas, lymph node, optic nerve, spinal cord, sciatic nerve of each group of rat, draw materials in same area, fix by 10% formalin.Medicine high dose group of the present invention and blank group Rats Organs and Tissues specimen are carried out tissue slice, carries out pathological examination, all the other specimen retain with for subsequent use.Result: through checking two treated animal organs and tissues specimen, each organ-tissue structure is all normal, and cell dyeing is even, without obviously changing.Because the checked organ-tissue of medicine high dose group animal of the present invention does not occur that medicine causes pathological change, therefore organ-tissue section is not carried out to dosage group in medicine of the present invention and low dose group.Tested organ-tissue form section photo and pathological section are reported attached.

Pathological observation result shows, the histiocyte of all multi viscera of each administration group rat is harmless, morphology is pointed out this medicine to each organs and tissues free of toxic effects.

5, conclusion

Rat chronic toxicity test result shows, with medicine 4.620g/kg, 2.310g/kg, 1.155g/kg body weight of the present invention, be equivalent to intend 60 times, 30 times, 15 times by clinical child's daily dose 0.077g/kg body weight, every twice-daily, rat oral gavage administration in continuous 13 weeks and drug withdrawal 2 weeks, compare with blank group; Each dosed administration is to the general state (mode of appearance, activity, stool shape etc.) of rat, growth promoter (body weight growth), hemopoietic function (hematological examination), Liver and kidney function (blood biochemical analysis), vitals ponderal index, all finds no obvious toxic action; Prove through pathological examination, child rejuvenates ball high dose group and blank group comparative observation, does not all find that obvious toxic damages changes to histoorgans such as the rat heart, liver, spleen, lung, kidney, adrenal gland, thymus, brain, hypophysis, uterus, ovary, testis, epididymis, prostate, thyroid, trachea, esophagus, stomach, aorta, duodenum, ileum, colon, bladder, pancreas, lymph node, optic nerve, spinal cord, sciatic nerves.Because obvious pathological change does not appear in each histoorgan of medicine high dose group animal of the present invention, therefore dosage group, low dose group in medicine of the present invention are not carried out to organ-tissue section and detected, its Saving specimen is for subsequent use.

In sum, long-term (13 weeks) administration of the high, medium and low Three doses of medicine of the present invention (4.620,2.310,1.155g/kg) and drug withdrawal are observed 2 weeks, to rat behavior activity, grow (body weight growth), food ration (feed consumption), blood cytology, blood biochemical analysis, organ index, organ-tissue morphology etc. all without remarkable toxic effect.Prompting Clinical practice safety.

experimental example 3 acute toxicity test

1 experiment material

1.1 medicine

Medicine of the present invention (being prepared by embodiment 1).

Usage and dosage: warm water is ground clothes or taken, below one full year of life each 1,1-2 year each 2,3-4 year each 3,5-7 year each 5,2-3 time on the one.Specification: every ball weighs 0.18 gram.

Dosage is arranged :oral, the dose 0.36-0.54g of day below one full year of life, 1-2 year day dose 0.72-1.08g, 3-4 year day dose 1.08-1.62g, 5-7 year day dose 1.8-2.7g.

This research with 1-3 year, day dose 1.08g counts, body weight by 3 × 2+8=14kg calculate, namely clinical consumption per day is 0.077g/kg.Face the used time is mixed with desired concn suspension oral gavage administration with distilled water.

1.2 animal

Kunming mouse, male and female half and half, body weight 12-14g, is provided by Military Medical Univ No.3, P.L.A's Experimental Animal Center, credit number: SCXK-(army) 2012-0003.

1.3 feedstuff

Feedstuff is full-valence pellet feed, is provided by above-mentioned unit.

1.4 zoopery places

Guiyang College of Traditional Chinese Medicine's pharmacological toxicology laboratory ventilation is good, sanitation and hygiene, temperature 20-25 DEG C, humidity 55%-65%.The other sub-cage rearing of animal unisexuality, feed of freely drinking water.

1.5 key instrument

ALC-210.3 type electronic balance, upper current chart level instruments and meters company limited.

1.6 statistical procedures

the poor opposite sex between data acquisition group, with ± Srepresent, carry out t inspection.

2 experimental techniques and result

2.1 prerun tests

With the pharmaceutical aqueous solution of the present invention of Cmax 20%, 0.4ml/10g body weight gavage gives mice, observes 3 days.Result: have no animal dead, shows to measure its LD ₅₀, therefore measure the maximum dosage-feeding of medicine of the present invention.

2.2 formal test

Get Kunming mouse 40, be divided into blank group, medicine group of the present invention at random, often organize 20, male and female half and half.Before experiment, water 12h is can't help in fasting, and medicine group gavage of the present invention gives the pharmaceutical aqueous solution 0.4ml/10g body weight of the present invention of mice 20%, and a twice-daily, is equivalent to 16g/kg body weight, is 208 times of clinical plan dosage 0.077g/kg body weight; Blank group with mutually commensurability normal saline gavage, a twice-daily.Continuous Observation 14d after administration, record animal has body weight change before and after non-toxic reaction and experiment, not produce dead dosage for maximum dosage-feeding.

Experimental result is in table 26.

Note: child rejuvenates, and ball group is female compares P>0.05 with blank group is female; Child rejuvenates, and ball group is male compares P>0.05 with blank group is male.

Result: after Kunming mouse gavage pharmaceutical aqueous solution 16g/kg of the present invention, grow healthy, fleshiness, glossy by Mao Mi, bright and flexible, the N/R secretions of eyes, crissum is clean, ingest normal, extremity are healthy and strong, and spontaneous activity is normal, in observation period, body weight increases gradually, compares no significant difference with blank group.Animal dead and toxicity is had no in 14d.At the end of experiment, naked eyes become celestial and have no obvious pathological change.

3 conclusions

In mice one day oral give 16g/kg body weight medicine of the present invention after, Continuous Observation 14 days, animal all survives, and mice outward appearance, behavior, breathing state, position and posture, to the reaction stimulated, substantially cuts open inspection and is showed no obvious abnormalities.Before medicine, after medicine the 7th day and 14 days medicine groups of the present invention are female, male mice body weight with there is no significant difference with sex blank group mice.

In this medicine Mouse oral of the present invention acute toxicity test, mice gives maximum dosage-feeding 16g/kg body weight, is equivalent to 208 times of clinical application amount, has no overt toxicity reaction.

Compared with prior art, the present invention adopts above-mentioned raw materials to make medicine, and each component raw material has good synergistic function, and the medicine made has heat-clearing and toxic substances removing, effect of expelling pathogenic factors from the exterior eliminating phlegm.For acute infantile convulsion, typhoid fever is generated heat, and faces fever at night, urine band blood, dimly visible not the going out of measles and cause fever of the body to cough; Bloody dysentery, watery diarrhea, food stagnation, stomachache.Evident in efficacy, side effect is little, reaches the object of invention.

Below in conjunction with embodiment, invention is further described, but not as the foundation limited the present invention.

detailed description of the invention:

Embodiment 1.

Formula: Rhizoma Coptidis 25g, Pulvis Cornus Bubali Concentratus 50g, Cornu Naemorhedi 25g, calamitas urinae hominis's (forging) 25g, Semen Sojae Preparatum 25g, Folium Isatidis 50g, Herba Schizonepetae 50g, Rhizoma Et Radix Notopterygii 50g, Radix Puerariae 50g, Radix Rehmanniae 50g, Caulis Clematidis Armandii 50g, Radix Paeoniae Rubra 50g, Radix Scutellariae 50g, Radix Peucedani 75g, Radix Scrophulariae 75g, Radix Platycodonis 75g, Radix Bupleuri 37.5g, Cacumen Tamaricis 37.5g, Rhizoma Cimicifugae 20g and Fructus Arctii (stir-fry) 75g.

Technique: get Cornu Naemorhedi and be ground into fine powder and Pulvis Cornus Bubali Concentratus mixes, for subsequent use; All the other medical materials are respectively got 2/3 amount and are ground into fine powder, sieve, and fine powder is for subsequent use; Coarse powder carries cream with residue medical material decocting in water, and cream and above-mentioned powder mix general ball, and dry, coating, to obtain final product.

Usage and dosage: oral, one-year-old following baby clothes 1, take 2 for 1 ~ 2 years old, within 3 ~ 4 years old, take 3, within 5 ~ 7 years old, take 5,2 ~ 3 times on the one.

Specification: often pipe fills 5, heavy 0.9g.

Embodiment 2.

Formula: Rhizoma Coptidis 85g, Pulvis Cornus Bubali Concentratus 120g, Cornu Naemorhedi 85g, calamitas urinae hominis's (forging) 85g, Semen Sojae Preparatum 85g, Folium Isatidis 120g, Herba Schizonepetae 120g, Rhizoma Et Radix Notopterygii 120g, Radix Puerariae 120g, Radix Rehmanniae 120g, Caulis Clematidis Armandii 120g, Radix Paeoniae Rubra 120g, Radix Scutellariae 120g, Radix Peucedani 150g, Radix Scrophulariae 150g, Radix Platycodonis 150g, Radix Bupleuri 90g, Cacumen Tamaricis 90g, Rhizoma Cimicifugae 60g and Fructus Arctii (stir-fry) 150g.

Technique: get Cornu Naemorhedi and be ground into fine powder and Pulvis Cornus Bubali Concentratus mixes, obtain mixed powder, for subsequent use; All the other medical material decocting in water carry cream, the cane sugar powder of cream and above-mentioned mixed powder and extractum amount 20% and the dextrin mixing granulation of 20%, dry, to obtain final product.

Specification: every 0.5g.

Usage and dosage: every day 2 times, each 3, oral.

Embodiment 3.

Formula: Rhizoma Coptidis 5g, Pulvis Cornus Bubali Concentratus 10g, Cornu Naemorhedi 5g, calamitas urinae hominis's (forging) 5g, Semen Sojae Preparatum 5g, Folium Isatidis 10g, Herba Schizonepetae 10g, Rhizoma Et Radix Notopterygii 10g, Radix Puerariae 10g, Radix Rehmanniae 10g, Caulis Clematidis Armandii 10g, Radix Paeoniae Rubra 10g, Radix Scutellariae 10g, Radix Peucedani 20g, Radix Scrophulariae 20g, Radix Platycodonis 20g, Radix Bupleuri 5g, Cacumen Tamaricis 5g, Rhizoma Cimicifugae 5g and Fructus Arctii (stir-fry) 20g.

Technique: get Cornu Naemorhedi and be ground into fine powder and Pulvis Cornus Bubali Concentratus mixes, obtain mixed powder, for subsequent use; All the other medical material decocting in water carry cream, and the starch of cream and above-mentioned mixed powder and extractum amount 20% mixes, tabletting, and film coating, to obtain final product.

Specification: every sheet 0.5g.

Usage and dosage: every day 2 times, each 10-20g, oral.

Claims (10)

1. treat the medicine of fever of children infantile convulsion for one kind, it is characterized in that: according to listed as parts by weight, primarily of Rhizoma Coptidis 5-85 part, Pulvis Cornus Bubali Concentratus 10-120 part, Cornu Naemorhedi 5-85 part, calamitas urinae hominis(calcined) 5-85 part, Semen Sojae Preparatum 5-85 part, Folium Isatidis 10-120 part, Herba Schizonepetae 10-120 part, Rhizoma Et Radix Notopterygii 10-120 part, Radix Puerariae 10-120 part, Radix Rehmanniae 10-120 part, Caulis Clematidis Armandii 10-120 part, Radix Paeoniae Rubra 10-120 part, Radix Scutellariae 10-120 part, Radix Peucedani 20-150 part, Radix Scrophulariae 20-150 part, Radix Platycodonis 20-150 part, Radix Bupleuri 5-90 part, Cacumen Tamaricis 5-90 part, Rhizoma Cimicifugae 5-60 part, Fructus Arctii (parched) 20-150 part and adjuvant are prepared from.

2. treat the medicine of fever of children infantile convulsion as claimed in claim 1, it is characterized in that: according to listed as parts by weight, primarily of Rhizoma Coptidis 10-40 part, Pulvis Cornus Bubali Concentratus 20-80 part, Cornu Naemorhedi 10-40 part, calamitas urinae hominis(calcined) 10-40 part, Semen Sojae Preparatum 10-40 part, Folium Isatidis 20-80 part, Herba Schizonepetae 20-80 part, Rhizoma Et Radix Notopterygii 20-80 part, Radix Puerariae 20-80 part, Radix Rehmanniae 20-80 part, Caulis Clematidis Armandii 20-80 part, Radix Paeoniae Rubra 20-80 part, Radix Scutellariae 20-80 part, Radix Peucedani 40-110 part, Radix Scrophulariae 40-110 part, Radix Platycodonis 40-110 part, Radix Bupleuri 20-60 part, Cacumen Tamaricis 20-60 part, Rhizoma Cimicifugae 10-30 part, Fructus Arctii (parched) 40-110 part and adjuvant are prepared from.

3. treat the medicine of fever of children infantile convulsion as claimed in claim 2, it is characterized in that: according to listed as parts by weight, be prepared from primarily of Rhizoma Coptidis 25 parts, Pulvis Cornus Bubali Concentratus 50 parts, Cornu Naemorhedi 25 parts, calamitas urinae hominis(calcined) 25 parts, Semen Sojae Preparatum 25 parts, Folium Isatidis 50 parts, Herba Schizonepetae 50 parts, Rhizoma Et Radix Notopterygii 50 parts, Radix Puerariae 50 parts, Radix Rehmanniae 50 parts, Caulis Clematidis Armandii 50 parts, Radix Paeoniae Rubra 50 parts, Radix Scutellariae 50 parts, Radix Peucedani 75 parts, Radix Scrophulariae 75 parts, Radix Platycodonis 75 parts, Radix Bupleuri 37.5 parts, Cacumen Tamaricis 37.5 parts, Rhizoma Cimicifugae 20 parts, Fructus Arctii (parched) 75 parts.

4. according to any one of claim 1-3, treat the preparation method of the medicine of fever of children infantile convulsion for one kind, it is characterized in that: get said medicine, conventionally extract, combine with conventional pharmaceutical adjuvants, also can not add adjuvant, make traditional drug formulations.

5. treat the preparation method of the medicine of fever of children infantile convulsion as claimed in claim 4, it is characterized in that: described pharmaceutical preparation is oral formulations.

6. treat the preparation method of the medicine of fever of children infantile convulsion as claimed in claim 5, it is characterized in that: described oral formulations comprises pill, granule, tablet or capsule.

7. treat the preparation method of the medicine of fever of children infantile convulsion as claimed in claim 6, it is characterized in that: described pill is prepared like this: get Cornu Naemorhedi and be ground into fine powder and Pulvis Cornus Bubali Concentratus mixes, for subsequent use; All the other medical materials are respectively got 2/3 amount and are ground into fine powder, sieve, and fine powder is for subsequent use; Coarse powder carries cream with residue medical material decocting in water, and cream and above-mentioned powder mix general ball, and dry, coating, to obtain final product.

8. treat the preparation method of the medicine of fever of children infantile convulsion as claimed in claim 6, it is characterized in that: described granule is prepared like this: get Cornu Naemorhedi and be ground into fine powder and Pulvis Cornus Bubali Concentratus mixes, obtain mixed powder, for subsequent use; All the other medical material decocting in water carry cream, the cane sugar powder of cream and above-mentioned mixed powder and extractum amount 20% and the dextrin mixing granulation of 20%, dry, to obtain final product.

9. treat the preparation method of the medicine of fever of children infantile convulsion as claimed in claim 6, it is characterized in that: described tablet is prepared like this: get Cornu Naemorhedi and be ground into fine powder and Pulvis Cornus Bubali Concentratus mixes, obtain mixed powder, for subsequent use; All the other medical material decocting in water carry cream, and the starch of cream and above-mentioned mixed powder and extractum amount 20% mixes, tabletting, and film coating, to obtain final product.

10. treat the preparation method of the medicine of fever of children infantile convulsion as claimed in claim 6, it is characterized in that: described capsule is prepared like this: get Cornu Naemorhedi and be ground into fine powder and Pulvis Cornus Bubali Concentratus mixes, obtain mixed powder, for subsequent use; All the other medical materials with 70% alcohol reflux, extracting solution reclaims ethanol and is also condensed into extractum, and the starch of extractum and above-mentioned mixed powder and extractum amount 20% and the magnesium stearate of 0.6% mixs, and with the alcohol granulation of 60%, drying, filling, to obtain final product.