CN103623192B - Medicine being used for the treatment of pig blue-ear disease and preparation method thereof - Google Patents

Abstract

The invention discloses a kind of medicine being used for the treatment of pig blue-ear disease and preparation method thereof, medicine comprises following raw material: Ganoderma, Herba of Artemisiae admsii, Radix Scutellariae, chopping board, pick up the ears, Herba Peperomiae pellucidae, bean pears, Herba Leonuri, Carnis Equi Asini, Bulbus Allii, Fructus Benincasae, Radix Salviae Miltiorrhizae, Fructus Liquidambaris, Radix Isatidis, Radix Glycyrrhizae, Foliumet Ramulus Evodiae, Radix Berberidis and Radix Zanthoxyli.The invention has the beneficial effects as follows: the present invention based on heat-clearing and toxic substances removing, with righting evil, anti-inflammation be auxiliary, provide a kind of medicine being used for the treatment of pig blue-ear disease, have evident in efficacy, side effect is low, and cure rate is high, the advantage not easily recurred.

Description

Medicine being used for the treatment of pig blue-ear disease and preparation method thereof

Technical field

The present invention relates to herbal medicine technical field, particularly a kind of medicine being used for the treatment of pig blue-ear disease and preparation method thereof.

Background technology

Primary disease was once called " mystery swine disease ", " new swine diseases ", " pig Epidemic abortion and breathing syndrome ", " pig reproduction and breathing syndrome ", " reproductive and respiratory syndrome ", " swine fever epidemic disease " etc., China is classified as two class infectious disease.PeterG.W.Plagemann speak from inference, this virus is that (lactic acid dehydrogenase increases virus by the arteritis virus of Mus, lactatedehydrogenase-elevatingvirus) variant viral that Central European wild boar (Wildboars) produces afterwards by wound infection, these wild boar Zeng Zuowei hunting uses are introduced into the U.S., the wild boar be introduced into just as intermediate host by virus disseminating to the North Carolina state, the time of infection approximately occurs in 1912 years.Autoheterosis and the evolution in this two continent of these viruses, approximately pass through seven in a decade or so, by contacting of family pig and wild boar, in cell entry man pig (domesticpigs) group, define two large independently reproductive and respiratory syndrome virus prototypes afterwards (genotype that two kinds of namely usually said reproductive and respiratory syndrome virus are basic): Europe class and american type.Europe and North America detect that the antibody of reproductive and respiratory syndrome virus also demonstrate that this point between 1991-1994 in wild boar body.Primary disease is a kind of high degree in contact sexually transmitted disease, in endemicity.PRRSV infected pigs, the pig of various kind, all ages and classes and purposes all can infect, but with in-pig and the most susceptible of the piglet within 1 monthly age.Ill pig and the malicious pig of band are the important sources of infection of primary disease.Major transmission path is that contact infection, air borne and seminal fluid are propagated, also by Placenta Hominis vertical transmission.Susceptible pig can per os, nasal cavity, muscle, abdominal cavity, the number of ways such as vein and intrauterine inoculation and infect virus, after pig infects virus 2 ~ 14 weeks all by contacting virus disseminating to other susceptible pigs.All virus can be detected from the nasal cavity of sick pig, feces and urine.Susceptible pig directly contacts with the malicious pig of band or has the means of transport of PRRSV, device contacts all can be infected with pollution.The flowing of infected pigs is also the important circulation way of primary disease.

The incubation period of primary disease differs greatly, and introduce susceptible swinery after infecting and the incubation period of PRRS occurs, the shortest is 3 days, and the longest is 37 days.The clinical symptom of primary disease alters a great deal, and by the impact of Strain, immune state and feeding and management factor and environmental condition.Low strain can cause popular without clinical symptom of swinery, and virulent strain can cause serious facing to examine disease.Morbidity sow main manifestations be that spirit is depressed, poor appetite or useless absolutely, heating, appearance dyspnea in various degree, latter half of gestation (105 ~ 107 days), sow is miscarried, premature labor, stillborn fetus, mummy tire, weak son.Sow abortion rate can reach 50% ~ 70%, and natimortality can reach more than 35%, and mummy can reach 25%, and part newborn piglet performance dyspnea, the symptom such as movement disorder and paresis, in 1 week puerperal, mortality rate obviously increases (40% ~ 80%).Minority sow shows as agalactia in puerperal, afterbirth is stagnated and vaginal secretions increases.1 the monthly age piglet show typical respiratory symptom, dyspnea, sometimes in ventral breathing, loss of appetite or absolutely useless, body temperature is elevated to more than 40 DEG C, diarrhoea.By hair slightly disorderly, ataxia, gradually becomes thin, blepharoedema.The visible ear of small part piglet, skin surface are blue, and before wean, piglet mortality rate can reach 80% ~ 100%, and after wean, the weightening finish of piglet reduces, and daily gain can decline 50% ~ 75%, and mortality rate raises (10% ~ 25%).Resistance to pig poor growth excessively, easy secondary other diseases.Grower pigs and growing and fattening pigs show slight clinical symptom, have the Respiratory symptoms of distinct program, and minority case can show cough and darkviolet appears in the ears back side, edge, abdominal part and tail skin.Easily there is secondary infection in infected pigs, and occurs corresponding symptom.The sickness rate of breeding boar is lower, and main manifestations is general clinical symptom, but the semen quality of boar declines, and deformity appears in sperm, and seminal fluid can be with poison.This is the principal mode showed at large-scale pig farm PRRS.Main manifestations is that the fertility performance of swinery declines, and poor growth, the reproductive performance of sow group declines, Swinery immunity function reduction, other bacillary and viral diseases of easy secondary infection.Respiratory tract disease (as mycoplasma infection, contagious pleuropneumonia, streptococcicosis, the eperythrozoonosis) sickness rate of swinery rises.

The defensive measure of pig blue-ear disease:

A. carry out medicinal health-care time flat, advise in care plans not using chloromycetin medicine (florfenicol), be cautious use of sulfonamide.Drug treatment, consider stress, toxic and side effects, immunosuppressant and many accompanying infections.

B. will often sterilize time flat, sterilization will use polytype disinfectant to carry out replacing and use.(as halogen, acids, aldehydes, bi-quaternary ammonium salt and biguanides, phenols and bases disinfectant) strengthens pig farm sterilization to prevention pig blue-ear disease is method a kind of very well.

C. not vaccination carelessly, the inoculated many vaccines in pig farm, healthy and stable on swinery is have critical impact.(as: oneself Seedling, PRRS Attenuate vaccine, PR Attenuate vaccine, swine fever spleen drench Seedling, also have the multi-joint Seedling etc. of import) wants that it is impossible for relying on vaccine to carry out preventing swine high fever, is finally beat more random and more random, the imbalance of last easily immunity of organism.

The resistance against diseases of rational nutrition, the enhancing body of D. arranging in pairs or groups.Ropy raw material had better not use, and avoids causing chronic poisoning, immunity degradation, brings out porcine hyperthermia.

E. notice that cooling is ventilated summer, pig farm health is clean.Require to prevent heat stress, heatstroke etc. from can bring out the environmental factors of pig blue-ear disease to swinery bath bathing.

F. the safe efficient remedy of pig blue-ear disease is controlled: with compound recipe anthocyanidin+taurine+aspirin/mix fodder.

But there is the shortcoming that cost is high, cure rate is low, mortality rate is higher in said medicine treatment pig blue-ear disease.Chinese herbal medicine is China's traditional medicine, for chemical synthetic drug, Chinese herbal medicine has plurality of active ingredients, not only have toxicity low, not easily have side effects, and Western medicine only has obvious curative effect to single disease or a class disease when treating, for the disease that some diseases are more, curative effect may be just not too obvious, usually need out a lot of Western medicine, price naturally can a lot of than Chinese herbal medicine and Chinese patent medicine costliness.

Summary of the invention

In order to realize foregoing invention object, the invention provides a kind of medicine being used for the treatment of pig blue-ear disease, described medicine comprises following raw material: Ganoderma, Herba of Artemisiae admsii, Radix Scutellariae, chopping board, pick up the ears, Herba Peperomiae pellucidae, bean pears, Herba Leonuri, Carnis Equi Asini, Bulbus Allii, Fructus Benincasae, Radix Salviae Miltiorrhizae, Fructus Liquidambaris, Radix Isatidis, Radix Glycyrrhizae, Foliumet Ramulus Evodiae, Radix Berberidis and Radix Zanthoxyli.

Described medicine is made up of the raw material of following parts by weight: Ganoderma 20-30 weight portion, Herba of Artemisiae admsii 10-17 weight portion, Radix Scutellariae 10-20 weight portion, chopping board 10-15 weight portion, to pick up the ears 10-20 weight portion, Herba Peperomiae pellucidae 10-20 weight portion, bean pears 10-30 weight portion, Herba Leonuri 15-20 weight portion, Carnis Equi Asini 10-15 weight portion, Bulbus Allii 10-15 weight portion, Fructus Benincasae 10-20 weight portion, Radix Salviae Miltiorrhizae 10-25 weight portion, Fructus Liquidambaris 13-20 weight portion, Radix Isatidis 10-20 weight portion, Radix Glycyrrhizae 10-20 weight portion, Foliumet Ramulus Evodiae 15-25 weight portion, Radix Berberidis 15-25 weight portion and Radix Zanthoxyli 10-20 weight portion.

Described medicine is preferably made up of the raw material of following parts by weight: Ganoderma 25-30 weight portion, Herba of Artemisiae admsii 12-17 weight portion, Radix Scutellariae 12-18 weight portion, chopping board 10-15 weight portion, to pick up the ears 13-17 weight portion, Herba Peperomiae pellucidae 12-18 weight portion, bean pears 15-25 weight portion, Herba Leonuri 15-20 weight portion, Carnis Equi Asini 10-15 weight portion, Bulbus Allii 10-15 weight portion, Fructus Benincasae 13-17 weight portion, Radix Salviae Miltiorrhizae 13-21 weight portion, Fructus Liquidambaris 15-20 weight portion, Radix Isatidis 12-17 weight portion, Radix Glycyrrhizae 12-18 weight portion, Foliumet Ramulus Evodiae 15-25 weight portion, Radix Berberidis 17-23 weight portion and Radix Zanthoxyli 12-20 weight portion.

Described medicine is preferably made up of the raw material of following parts by weight: Ganoderma 28 weight portion, Herba of Artemisiae admsii 15 weight portion, Radix Scutellariae 16 weight portion, chopping board 13 weight portion, to pick up the ears 15 weight portions, Herba Peperomiae pellucidae 15 weight portion, bean pears 21 weight portion, Herba Leonuri 18 weight portion, Carnis Equi Asini 13 weight portion, Bulbus Allii 12 weight portion, Fructus Benincasae 15 weight portion, Radix Salviae Miltiorrhizae 17 weight portion, Fructus Liquidambaris 17 weight portion, Radix Isatidis 15 weight portion, Radix Glycyrrhizae 16 weight portion, Foliumet Ramulus Evodiae 20 weight portion, Radix Berberidis 18 weight portion and Radix Zanthoxyli 17 weight portion.

In order to better realize foregoing invention object, present invention also offers a kind of preparation method being used for the treatment of pig blue-ear disease medicine, the dosage form of described medicine is injection, and its preparation method comprises the following steps:

The first step, takes all raw materials by weight and obtains mixture, decoct with water 2 ~ 4 times, each decocting time is 2 hours ~ 3 hours, the water quality added is 2 ~ 4 times of mixture quality, merges decoction liquor, filters, filtrate is concentrated into syrupy shape, add the ethanol that determining alcohol is more than 95%, the ethanol content added is 1 ~ 2 times of mixture quality, leaves standstill after 12 hours ~ 24 hours, centrifugal filtration, filtrate is adjusted to filtrate pH7 ~ 8 with the NaOH of 40% ~ 50%;

Second step, by the filtrate that the first step obtains, adds 0.5% ~ 1% active carbon and boils 18 ~ 22 minutes, filters, decarburization, injects with water, makes the crude drug concentration of injection be 2g/ml ~ 4g/ml, fill sterilizing 1.5 hours ~ 2 hours in 500ml saline bottle, after freezing a couple of days, filters.

Present invention also offers a kind of preparation method being used for the treatment of pig blue-ear disease medicine, the dosage form of described medicine is powder, and its preparation method comprises the following steps:

The first step, take the mixing of all raw materials by weight, be ground into powder, load in multi-function extractor, adding relative to the determining alcohol of mixture quality 3 ~ 5 times is the ethanol of 80% ~ 90%, and reflux is extracted for 1 hour ~ 3 hours, obtains extracting solution, steam pressure in reflux process is 0.4MPa ~ 0.6MPa, extracting liquid filtering is obtained the first filtrate and filtering residue;

Second step, adding relative to the determining alcohol of first step mixture quality 1 ~ 3 times by the filtering residue that the first step obtains is the ethanol of 80% ~ 90%, reflux is extracted for 0.5 hour ~ 1.5 hours, obtain extracting solution, steam pressure in reflux process is 0.4MPa ~ 0.6MPa, extracting liquid filtering is obtained the second filtrate;

3rd step, the first filtrate and the second filtrate are merged, concentrating under reduced pressure is except desolventizing, obtain and get dry extract, dry cream is put into spray dryer dry, ensure that inlet temperature is at 170 DEG C ~ 190 DEG C, leaving air temp 60 DEG C ~ 80 DEG C, is ground into 200 order ~ 400 orders subsequently, obtains powder.

The beneficial effect that the technical scheme that the embodiment of the present invention provides is brought is: the present invention based on heat-clearing and toxic substances removing, with righting evil, anti-inflammation be auxiliary, provide a kind of medicine being used for the treatment of pig blue-ear disease, have evident in efficacy, side effect is low, and cure rate is high, the advantage not easily recurred.

Detailed description of the invention

From Chinese veterinarian's angle, pig blue-ear disease belongs to Epidemic Febrile Disease, " exogenous pathogen mutual aid " be the five internal organs that predisposing factors i.e. the traditional Chinese medical science are said when being in " void " virus take advantage of a weak point, cause evil victory just to decline, disease-resistant unable; This disease is multiple for summer, how because of the damp and hot fire-transformation of diseases caused by exogenous pathogenic factor, causes inhibited pulmonary QI, abnormal ascending-descending of QI, imbalance of YIN and YANG.Case changes mainly lung and pleura and sticks together, lung swelling, and lung has dispersivity hemorrhage, in interstitial pneumonia, and some lung atrophys, in carnification, serious loss gas exchange capacity; The cystose mucus of volume or faint yellow purulence mucus is had in trachea-bronchial epithelial cell.The remarkable swelling of inguinal lymph nodes, tangent plane is moistening turns up, congested, seriously hemorrhage, mesenteric lymph node, hilar lymph node obvious tumefaction, in khaki or kermesinus.Treatment with heat-clearing and toxic substances removing, vigorate qi and replenish the blood, anti-inflammation, should strengthen pig resistance

According to above-mentioned theory and principle, the invention provides a kind of medicine for the treatment of pig blue-ear disease, comprise Ganoderma, Herba of Artemisiae admsii, Radix Scutellariae, chopping board, pick up the ears, Herba Peperomiae pellucidae, bean pears, Herba Leonuri, Carnis Equi Asini, Fructus Benincasae, Radix Salviae Miltiorrhizae, Fructus Liquidambaris, Radix Isatidis, Radix Glycyrrhizae, Foliumet Ramulus Evodiae, Radix Berberidis and Radix Zanthoxyli.

Wherein, the pharmacological property of each raw material is as follows:

Ganoderma: property is sweet, flat.GUIXIN, lung, liver, kidney channel.Cure mainly asthenia, cough, asthma, insomnia, dyspepsia, malignant tumor etc.Animal pharmacology shows experiment: Ganoderma has inhibitory action to nervous system, and blood circulation has blood pressure lowering and strengthens the effect of cardiac contractile force, has phlegm-dispelling functions to respiratory system, in addition, also has hepatoprotective, improves immunologic function, antibacterially waits effect.

Herba of Artemisiae admsii: bitter, cold.Function cures mainly: heat-clearing and toxic substances removing, stomach invigorating.Cure mainly pneumonia, gastritis, laryngitis, tonsillitis, conjunctivitis.

Radix Scutellariae: have heat clearing and damp drying, removing heat from blood is antiabortive, detoxicating functions.Cure mainly the diseases such as epidemic febrile disease, upper respiratory tract infection, cough due to lung-heat, damp and hot jaundice, pneumonia, dysentery, hemoptysis, conjunctival congestion, frequent fetal movement, hypertension, carbuncle furuncle.The clinical practice of Radix Scutellariae is antibacterial not worse than Rhizoma Coptidis, and does not develop immunity to drugs.We are by the strong feature of broad-spectrum antibacterial action, are used in fungal culture miscellaneous bacteria and infect special severe, fine by Radix Scutellariae extracting solution effect." Bencao Jingshu ": Radix Scutellariae, its property is respectful clearly, so evil removing: bitter in the mouth is so dampness; YIN-cold is so victory heat, therefore main all heat.All hot persons, pathogenic heat with all over heat also, jaundice, intestinal be out-of-the-way, let out dysentery, and the disease of all damp and hot victory also, analyses it, then all diseases are from recover.Bitter cold can except damp and hot, thus small intestinal profit and water from by, source is clearly then flowed also clean.The blood person of closing, excess-heat is at blood system, and instant heating human blood room, it is obstructed to make us amenorrhea, and damp and hot solution, then Ying Qi clearly and voluntarily also.Malignant boil cellulitis erosion person, heat in blood then stays knot, and is that carbuncle stain is also rotten: acute scleritis person, also, removing heat from blood heat extraction, then spontaneous recovery also for fire of moxibustion blood trouble.

Chopping board: bitter in the mouth, cold in nature, GUIXIN, stomach warp, function clearing away heat and cooling blood, be removing toxic substances key medicine, be mainly used in pyretic toxicity and send out speckle, erysipelas, laryngopharynx swelling and pain, aphtha of the mouth and tongue, enter all diseases caused by blood system and pyretic toxicity for warm pathogenic factor, return stomach warp, effect is heat clearing away.

Pick up the ears: pungent; Sweet; Warm in nature.Return liver; Kidney channel.Function cures mainly: wind-evil dispelling and cold-evil expelling; Relaxing muscles and tendons and activating QI and blood in the collateral; Kidney invigorating and YANG supporting.Main lumbago and skelalgia; Numb hand and foot; Grain does not relax; Impotence and seminal emission, weakness of the waist and knees.1. anticancer have two kinds of polysaccharide with active anticancer in oyster cap fungus, and master is acidic polysaccharose of picking up the ears, containing β-1,3-glucosan 69%, galactose 13%, mannose 6%, glucuronic acid 13%, rear three is all present in polysaccharide structures with side chain, and hot water extract is 91% to the suppression ratio of euphorbia egg decoctum.A kind of glycoprotein extracted from oyster cap fungus is external has stronger cytotoxicity to S180, ascites tumor ECA, hepatocarcinoma Hepa and Human B lymphoma cell Raji, K562 Leukaemia, and selective to different carcinoma cell.In vivo test shows, this component has the effect suppressing mice S180 tumor cell proliferation and tissue invasion towards periphery, tumor tissue can be made downright bad, lymphocytic infiltration, and the fibrous capsule that formation is thicker, point out it to have and make the Fibrotic effect of tumor tissues.2. strengthen immunity: the glycopeptide component be separated from From Pleurotus Ostreatus Fungi, have and promote that Lymphokine kills and wounds the effect of (LAK) cell and NKT (NK) cell killing tumor.3. blood fat reducing and prevent and treat atherosclerosis: pick up the ears to extract extractum, vacuum drying by water and different concentration ethanol.Be added in hamster high lipid food with 3% alcohol extractum, after 6 weeks, the extractum of water and 30% and 60% ethanol extraction, obviously can alleviate serum and liver cholesterol and three ether glycerol (triglyceride).

Herba Peperomiae pellucidae: pungent; Cool.Gui Jing: liver; Lung meridian.Function cures mainly: eliminating stasis to stop pain; Heat-clearing and toxic substances removing.Main carbuncle sore tumefacting virus; Burn and scald; Traumatic injury; Traumatic hemorrhage.

Bean pears: micro-sweet, puckery, cool.Nourishing the lung to arrest cough, heat-clearing and toxic substances removing.Cure mainly xeropulmonary cough, acute ocular conjunctivitis.

Herba Leonuri: bitter in the mouth, pungent, cold nature, enters liver, pericardium channel, has promoting blood flow to regulate menstruation, inducing diuresis to remove edema, the effect of heat-clearing and toxic substances removing, for the menoxenia caused by blood stasis, dysmenorrhea, amenorrhea, puerperal abdonimal pain or lochiorrhea; Edema, dysuria; Traumatic injury, the cards such as sore and toxic.

Carnis Equi Asini: sweet acid is flat.Function cures mainly: enrich blood, QI invigorating.Control strain, dizziness due to wind pathogen, vexed.

Bulbus Allii: warm in nature, acrid in the mouth is put down; Enter spleen, stomach, lung meridian.Removing toxic substances parasite killing, reducing swelling and alleviating pain, antidiarrheal dysentery relieving, controls lung, anthelmintic, also has warming the spleen warming the stomach in addition.Control swollen ulcer drug, white bald tinea skin ulcer, dysentery is had loose bowels, pulmonary tuberculosis pertussis, ascarid pinworm, eating accumulation, coldness and pain in the epigastrium, edema distension, circulation of qi promoting removing food stagnancy, and parasite killing detoxifies.For flu, bacillary dysentery, amebic dysentery, enteritis, eating accumulation, carbuncle skin infection.Strong disinfecting: in Bulbus Allii, sulfur-containing compound has strong antibacterial and anti-inflammation functions, all has suppression and killing action to multiple coccus, bacillus, fungus and virus etc., is the one that in the natural plants found at present, antibacterial action is the strongest.Anti-curing oncoma and cancer: the elements such as the germanium in Bulbus Allii and selenium can the growth of inhibition tumor cell and cancerous cell, and experiment finds, the crowd that cancer incidence is minimum is exactly the crowd that in blood, selenium content is the highest.National Cancer tissue is thought, in the plant of the anticancer potentiality of whole world most, what rank first is Bulbus Allii.Toxin-expelling intestine-cleaning, prevention of gastrointestinal: Bulbus Allii can effectively suppress and kill the bacterial viruses such as the helicobacter pylori causing gastroenteropathy, removes the intestines and stomach noxious substance, stimulating gastrointestinal mucosa, promotes appetite, accelerates digestion.Reduce blood glucose, prevent diabetes: Bulbus Allii can promote the secretion of insulin, increase histiocyte to the absorption of glucose, improve human glucose tolerance, blood sugar level in rapid reduction body, and the various pathogenic bacterias because of infection-induced diabetes can be killed, thus effective prevention and therapy diabetes.Control cardiovascular and cerebrovascular disease: Bulbus Allii can prevent the lipidosis in cardiovascular and cerebrovascular vessel, the metabolism of induced tissue fat inside, remarkable increase fibrinolytic activity, reduces cholesterol, suppresses hematoblastic gathering, reduce plasma concentration, increase arteriolar expansion ratio, impel vasodilation, regulate blood pressure, increase the permeability of blood vessel, thus suppress formation and the prevention of arterial sclerosis of thrombosis.Eat 2 ~ 3 lobe Bulbus Alliis every day, be the preferably the most easy way of blood pressure lowering, Bulbus Allii can help the right quantity of a kind of enzyme in holder and avoid occurring hypertension.

Fructus Benincasae: sweet light, cool.Enter lung, intestine and small intestine, urinary bladder channel.Function cures mainly: diuretic, expectorant, heat clearing away, removing toxic substances.Harness the river swollen, distension, beriberi, gonorrhea, expectorant is shouted, cough with asthma, and summer-heat is unhappy, quenches one's thirst, dysentery, carbuncle, anal fistula; And separate ichthyotoxin, alcoholism.

Radix Salviae Miltiorrhizae: there is promoting blood circulation to remove blood stasis, detumescence hemostasis, anti-inflammatory analgetic, menstruction regulating and pain relieving, coronary artery dilator, improve myocardial ischemia situation, reduce blood pressure, calm the nerves with all worries set aside, blood sugar lowering and the effect such as antibacterial, to menoxenia, amenorrhea dysmenorrhea, lump in the abdomen, breast ventral spine pain, pyretic arthralgia pain, skin infection swells and ache, dysphoria and insomnia; Hepatosplenomegaly, the diseases such as angina pectoris have certain curative effect.In addition, modern medicine experiment proves, Radix Salviae Miltiorrhizae also has anti-platelet aggregation, reduces the function of blood viscosity and the inside and outside blood coagulation system of adjustment, is the natural Chinese medicine that a kind of safety treats again cardiovascular disease reliably.For costa sternales hypochondriac pain, rheumatic arthralgia, lump in the abdomen lumps, and skin infection swells and ache, and tumbling down is grieved, menoxenia, amenorrhea dysmenorrhea, puerperal stasis of blood pain etc.Treatment costa sternales pain, lump in the abdomen caking, and menoxenia, amenorrhea dysmenorrhoea have good effect, normal and Rhizoma Chuanxiong compatibility is applied.Belonging in qi depression to blood stasis in treatment breast abdomen pain, often coordinating the medicine such as Fructus Amomi, Lignum Santali Albi with using.Enter nutrient blood for epidemic febrile disease heat, body sends out macule, coma is irritated.

Fructus Liquidambaris: bitter is flat; Return liver, kidney channel." detailed outline is picked up any lost article from the road ": " maple fruit, tree like Cortex Populi dividianae, inner circle as honeycomb, i.e. Fructus Liquidambaris.The large acupoints of twelve meridians that can pass through of its property, therefore " the lifesaving sea of bitterness " harnesses the river, swelling is with it, can search by FUSHUI also with it." " modern Practical Chinese medicinal ": " ashing is outer for skin exudative dermatitis, anal fistula etc., has convergence, antiinflammatory, disinfective action." dispelling, collateral-activating, diuretic stimulates the menstrual flow.For arthralgia, numbness contracture, edema distension, the few amenorrhea of breast.The property of Fructus Liquidambaris tool tonneau, function promoting the circulation of QI to alleviate the stagnation in middle-JIAO and pain relieving, clinically for abdominal distention, the disease such as not well of defecating.

Radix Isatidis: resisting pathogenic microbes effect: Radix Isatidis has inhibitory action to various bacteria.Infusion has inhibitory action in various degree to bacillus subtilis, staphylococcus aureus, sarcine, escherichia coli, Bacillus typhi, Salmonella paratyphi, dysentery (will congratulate, Fu Shi) bacillus, bacillus enteritidis etc.; Acetone leachate also has similar effect, and to Hemolytic streptococcus effectively (all using agar aperture flat band method).Similar to the bacteriostasis of A type meningococcus and Bulbus Allii, Flos Lonicerae. infected by influenza PR8 strain and Jing Ke 68-1 strain have obvious inhibitory action.In vitro leptospiral effect killed by Radix Isatidis.To cardiovascular effect: these product can make the in vitro rabbit ear and rat lower limb perfusion flow increase, and improve Rabbit Mesentery microcirculation, reduce the blood pressure of Anaesthetic Rabbits, reduce capillary permeability, and make mouse cardiac muscle oxygen consumption decline.Anticoagulant effect Radix Isatidis has remarkable inhibitory action to the Platelet Aggregation in Rabbits that ADP induces.From Radix Isatidis, get uridnine, hypoxanthine, pyrimidine, salicylic acid etc. also all show certain inhibit activities to the Platelet Aggregation in Rabbits that ATP induces, when 0.21mg/kg concentration, its suppression ratio is respectively 18.2%, 24.2%, 10.8% and 20%.Detoxication: dog Radix Isatidis, Rhizoma Coptidis powder and Rhizoma et radix veratri (Radix Rhizoma Veratri), with taking (each 2.0g/kg), can separate Rhizoma et radix veratri (Radix Rhizoma Veratri) poison, reduces mortality rate; If again with it after veratrum poisoning, then invalid; Alone Radix Isatidis powder or Rhizoma Coptidis powder respectively, effect is also bad." book on Chinese herbal medicine is stated ": bitter, cold, nontoxic." book on Chinese herbal medicine is just read ": enter liver, stomach blood system." China's book on Chinese herbal medicine ": return liver; Stomach warp.There is heat clearing away, removing toxic substances, removing heat from blood, sore-throat relieving effect.Treatment maculae caused by violent heat pathogen; High heat headache; Major part pestilence; Crimson tongue is purple dark, scarlet fever; Erysipelas; Mumps; Sore throat; Skin ulcer is swollen, carbuncle; Chickenpox; Measles; Hepatitis; Influenza, epidemic encephalitis, encephalitis b, pneumonia, coma tells nosebleed, pharyngeal swelling, acute catarrhal conjunctivitis, skin ulcer rash; Epidemic encephalitis type B, acute, chronic hepatitis, mumps, osteomyelitis can be prevented and treated.

Radix Glycyrrhizae: nature and flavor are sweet, flat.Enter spleen, stomach, lung meridian.Can QI invigorating invigorating middle warmer; Relieving spasm to stop pain; Nourishing the lung to arrest cough; Eliminating fire and detoxication; Coordinating the actions of various ingredients in a prescription.Main asthenia lack of appetite; Leanness yellowish complexion; Shortness of breath and palpitation; Stomachache loose stool; The anxious pain of extremity contraction; Hysteria; Cough and asthma; Laryngopharynx swelling and pain; Carbuncle skin ulcer swells and ache; Infantile carbuncle; And medicine, alimentary toxicosis.For weakness of the spleen and stomach, fatigue and weakness, shortness of breath and palpitation, cough with copious phlegm, the anxious pain of gastral cavity abdomen, extremity contraction, carbuncle sore tumefacting virus, cushion toxicity, strong." herbal classic " is said: " main the five internal organs six mansion cold and heat pathogen, hard muscles and bones, longue meat, times power, incised wound swells, removing toxic substances ".People's the moon (no) " property of medicine opinion " is said: " in main abdomen, cold type of pain, controls infantile convulsion, except distention and fullness in the abdomen; Tonification the five internal organs; Make all poison of drug; Support kidney qi internal injury, make flaccidity; Housewife's human blood drop lumbago; Empty and frequent fever; Add and with it ".Western modern medicine haircut is existing, and Radix Glycyrrhizae agent has the function of antiinflammatory and the reflection of resistance state, therefore in doctor trained in Western medicine clinically mainly as demulcent.Relieving cough, eliminates the phlegm, treatment pharyngalgia laryngitis; Radix Glycyrrhizae or enoxolone have the effect of desoxycortone class, have good effects to chronic adrenocortical hypofunction; Glycyrrhiza preparation can promote stomach mucus to be formed and secretion, extends the epithelial cell life-span, has anti-inflammatory activity, be usually used in the treatment of chronic ulcer and duodenal ulcer; The flavone of Radix Glycyrrhizae has antiinflammatory, spasmolytic and antiacid effect; Radix Glycyrrhizae is also one of primary raw material of rendan mini-pills.

Foliumet Ramulus Evodiae: bitter, cold.Function cures mainly: heat-clearing and toxic substances removing, eliminating stasis to stop pain.Prevent and treat influenza, epidemic cerebrospinal meningitis, encephalitis B, heatstroke, cure cold high heat, tonsillitis, pharyngolaryngitis, lung abscess, pneumonia, malaria, rheumatic arthritis, sciatica, lumbago and skelalgia, stomachache, icterohepatitis, Gelsemium elegans Benth. (Herba Gelsemii Elegantis) is poisoning.Osteopatia sprain is controlled in external, worm venom, carbuncle furuncle and phyma poison, trauma infection contamination, eczema, dermatitis.

Radix Berberidis: bitter, cold.Cure mainly heat clearing and damp drying, eliminating fire and detoxication.For the colorectal cancer of hyperactivity of toxic heat, disease sees that times of defecation increases, with pus and blood mucus time just, and abdominal part distending pain, burning sensation of the anus etc., the fit applications such as normal and Herba Patriniae, Herba Hedyotidis Diffusae, Cortex Phellodendri.Stop up the pulmonary carcinoma of containing for expectorant heat, disease sees heating, chest pain, out of breath, thirsty, constipation, cough with copious phlegm etc., often coordinates should have with Herba Taraxaci, Cortex Lycii, Folium Pyrrosiae, Herba Gnaphalii Affinis etc.For tumor patient through radiotherapy, Leukocytes after Chemotherapy reduces the first blood medicine compatibility of the supplementing QI and nourishing YIN such as normal and the Radix Astragali, Radix Angelicae Sinensis, Fructus Ligustri Lucidi, Radix Scrophulariae and applies.

Radix Zanthoxyli: arduous, temperature, slightly poisonous.Dispel the wind, dredging collateral, detumescence, pain relieving.Dispelling the wind and dampness pathogens osteodynia, sore throat, scrofula, stomachache, toothache, traumatic injury, soup, burning hot wound.1. " herbal classic ": " main anemofrigid-damp arthralgia, severe and migratory arthralgia aches, except four cold of the limbs gas, gonalgia." 2. " prescriptions worth thousand gold ": " harnesses the river, and the whole body is swollen, and pig green pepper branch and leaf are decocted as treacle, (medicine) being taken before meal one an ancient type of spoon, day three.Itch, wash it with juice." 3. " Treatise on Dietetic Therapy ": " main harmful wind contraction is anxious." 4. " herbal classic is met former ": " energy qualcomm meridian, goes pathogenic wind toxic, arthralgia chiefly caused by damp pathogen." 5. " book on Chinese herbal medicine asks former ": " is controlled phlegm-fire and is stolen core, and anxious larynx unconsciousness due to blockage of phlegm critically ill, remove crust, decoct diseases caused by retention of fluid." 6. " south of the Five Ridges gather medicinal herbs record ": " reason traumatic injury and snakebite.Suffer from toothache, decoct water rinsing the mouth." 7. " Luchuan book on Chinese herbal medicine ": " synthetism, detumescence, pain relieving, blood stasis removing.Control injury and bone fracture, damage is swollen and ache, rheumatic ostalgia, heart peratodynia, toothache.And treating snakebite." 8. Guangzhou army " conventional Chinese herbal medicine handbook ": " dispelling, collateral-activating.Control lumbar muscle strain, tetanus, soup, burning hot wound."

Embodiment 1 injection

Embodiments provide a kind of medicine being used for the treatment of pig blue-ear disease, be made up of the raw material of following parts by weight: Ganoderma 28 weight portion, Herba of Artemisiae admsii 15 weight portion, Radix Scutellariae 16 weight portion, chopping board 13 weight portion, 15 weight portions of picking up the ears, Herba Peperomiae pellucidae 15 weight portion, bean pears 21 weight portion, Herba Leonuri 18 weight portion, Carnis Equi Asini 13 weight portion, Bulbus Allii 12 weight portion, Fructus Benincasae 15 weight portion, Radix Salviae Miltiorrhizae 17 weight portion, Fructus Liquidambaris 17 weight portion, Radix Isatidis 15 weight portion, Radix Glycyrrhizae 16 weight portion, Foliumet Ramulus Evodiae 20 weight portion, Radix Berberidis 18 weight portion and Radix Zanthoxyli 17 weight portion.

Wherein, the dosage form of medicine is injection, and its preparation method comprises the following steps:

The first step, takes all raw materials by weight and obtains mixture, decoct with water 3 times, each decocting time is 2.5 hours, the water quality added is 3 times of mixture quality, merges decoction liquor, filters, filtrate is concentrated into syrupy shape, add the ethanol that determining alcohol is more than 95%, the ethanol content added is 2 times of mixture quality, leaves standstill after 18 hours, centrifugal filtration, filtrate is adjusted to filtrate pH7 with the NaOH of 50%;

Second step, by the filtrate that the first step obtains, adds 1% active carbon and boils 20 minutes, filters, decarburization, injects with water, makes the crude drug concentration of injection be 3g/ml, and fill sterilizing 2 hours in 500ml saline bottle, after freezing a couple of days, filters, obtain injection.

Toxicity test

(1) animal is selected

Cleaning grade kunming mice 40, male and female half and half, body weight 18 ~ 22g; Laboratory observation 1 week, before each administration, fasting 4h, can't help water.

(2) test grouping

By above cleaning grade kunming mice laboratory observation 1 week, the cleaning grade kunming mice of clinical observation result health is divided into two groups at random, often organizes 20 (male and female half and half).Wherein one group is set as experimental group, and another group is set as matched group.

(3) medication and dosage

Mouse tail vein injection, experimental group is with successive administration in maximum (former) concentration of the embodiment of the present invention 1 and maximum volume 24h 3 times, give 0.6ml/20g(Mouse Weight) medicine, i.e. the injection 1.8g(crude drug amount of mouse tail vein injection embodiment 1 in 24h)/20g(Mouse Weight); Matched group is to the injection normal saline of same volume.

(4) observational technique and index

Weigh the weight of animals before administration and observe feed intake and drinking-water situation; After administration, every day weighs the weight of animals, feed intake and undue toxicity's symptom at same time, Continuous Observation 14d.Observation index is as follows: 1. take action, as unease, many dynamic, sounding; 2. nervous system reaction, as lifted tail, chatter, spasm, movement disorder, attitude extremely; 3. autonomic nervous system reaction, as exophthalmos, sialorrhea, shed tears, urinate, drop, perpendicular hair, dyschromasia, adnormal respiration; 4. drinking-water and appetite change, defecation and color thereof, nose, eye, oral cavity whether with or without abnormal secretion thing, body weight change; 5. death condition.

(5) experimental result

By the observation of 14d, two groups all do not have undue toxicity to react appearance, do not occur yet animal dead phenomenon.

Embodiment 2 powder

Embodiments provide a kind of medicine being used for the treatment of pig blue-ear disease, medicine is made up of the raw material of following ratio of weight and number: Ganoderma 30 weight portion, Herba of Artemisiae admsii 17 weight portion, Radix Scutellariae 12 weight portion, chopping board 15 weight portion, 13 weight portions of picking up the ears, Herba Peperomiae pellucidae 18 weight portion, bean pears 25 weight portion, Herba Leonuri 15 weight portion, Carnis Equi Asini 12 weight portion, Bulbus Allii 10 weight portion, Fructus Benincasae 17 weight portion, Radix Salviae Miltiorrhizae 21 weight portion, Fructus Liquidambaris 20 weight portion, Radix Isatidis 12 weight portion, Radix Glycyrrhizae 18 weight portion, Foliumet Ramulus Evodiae 15 weight portion, Radix Berberidis 17 weight portion and Radix Zanthoxyli 12 weight portion.

Medicine is powder, and its preparation method comprises the following steps:

The first step, take the mixing of all raw materials by weight, be ground into powder, load in multi-function extractor, adding relative to the determining alcohol of mixture quality 4 times is the ethanol of 85%, and reflux is extracted for 2 hours, obtains extracting solution, steam pressure in reflux process is 0.5MPa, extracting liquid filtering is obtained the first filtrate and filtering residue;

Second step, adding relative to the determining alcohol of first step mixture quality 2 times by the filtering residue that the first step obtains is the ethanol of 80%, and reflux is extracted for 1 hour, obtains extracting solution, steam pressure in reflux process is 0.5MPa, extracting liquid filtering is obtained the second filtrate;

3rd step, the first filtrate and the second filtrate are merged, concentrating under reduced pressure, except desolventizing, obtains and gets dry extract, and dry cream is put into spray dryer dry, and ensure that inlet temperature is at 180 DEG C, leaving air temp 70 DEG C, is ground into 300 orders subsequently, obtains powder.

Toxicity test

Select SD rat, give the powder lysate of the embodiment of the present invention 2 of variable concentrations (18.0,6.0,2.0g crude drug/kg), every day gavage once, continuous 90 days, within after last administration 24 hours, respectively group is alive kills 1/2 animal (male and female half and half), and all the other 1/2 animals continue observations 2 weeks rear living and kill.Duration of test observes outward appearance, general behavior, food ration, the body weight change of animal, within after administration 90 days, within 2 weeks, carries out the index checking such as hematology's (RBC, HB, reticulocyte, PLT, CT, WBC and classification) and blood biochemical (AST, ALT, ALP, Glu, BUN, Crca, TP, T.BIL, ALB, CHOL), urine biochemistry, organ coefficient, histopathology with drug withdrawal.Result of the test shows: Chinese medicine preparation of the present invention is good at high, medium and low dosage treated animal general state, and outward appearance sign, behavioral activity, food-intake and body weight increase change all without exception; Three dosage groups and matched group hematological examination, blood biochemical analysis, urine biochemical analysis all in normal range, without significant difference between group; Each group of main organs histopathological examination shows no obvious abnormalities.These parameters drug withdrawal also had no change after 2 weeks.This test dosage is respectively 180,60,20 times of clinical medicine dose, rat is had no significant effect in high, medium and low three dosage (18.0,6.0,2.0g crude drug/kg) administration in continuous 90 days according to result of the test Chinese medicine preparation of the present invention, without clear and definite toxicity target organ and sensitive indicator, convalescent period observation also has no retardance toxic reaction, and the dose safety of the clinical practice of the prompting embodiment of the present invention 2 powder is higher.

Clinical trial

1.1 for pilot production material

1.1.1 for examination animal

20 age in days piglets 150, purchased from pig farm, Shandong, on inspection, antibody and the antigen of swine fever, pig lung plague, pig erysipelas, pig streptococcicosis, pig blue-ear disease are feminine gender.Isolated rearing is in clean sterilization colony house.

1.1.2 for prelibation strain

HP-PRRSV(TCID ₅₀be 1 × 10 ⁶) strain provides by China Veterinery Drug Inspection Office.

1.1.3 medicine

Injection prepared by embodiment 1, crude drug amount is 3g/ml; Powder prepared by embodiment 2, is mixed into feedstuff according to the 5-10% of feedstuff gross weight; Drink in compound recipe anthocyanidin 1000 grams+taurine 2000 grams+aspirin 1000 grams/1000 kilograms clean waters.

1.2 test method

1.2.1 inoculation test

Give often group test pig trachea injection HP-PRRSV, 3mL/ head.What occur pig blue-ear disease symptom after counteracting toxic substances in three days has 122, selects 120 ill pigs as experiment pig.

1.2.2 grouping is tested

120 ill piglets are divided into three groups at random, often organize 40, i.e. embodiment group 1, embodiment group 2 and matched group.

1.2.2 test method

Occur the pig of pig blue-ear disease symptom after counteracting toxic substances, embodiment group 1 uses the injection intramuscular injection for treating of the embodiment of the present invention 1, and once, each 5mL/ head, uses three days continuously in injection every day; Embodiment group 2 uses the powder of the embodiment of the present invention 2, is mixed into by powder in feedstuff, conventionally feeds, and each mixed volume is the 5-10% of feedstuff gross weight, uses three days continuously; Matched group uses in compound recipe anthocyanidin 1000 grams+taurine 2000 grams+aspirin 1000 grams/1000 kilograms clean waters and drinks, three days on probation continuously.

1.2.3 observed and recorded

Observe clinical symptoms and the pathological change of each group of duck after counteracting toxic substances, and compare its order of severity, record counteracting toxic substances after death toll, three days treatment terminate after therapeutic effect.

2 results

2.1 criterion of therapeutical effect

Cure: ill temperature of pig body, body colour, feces, spirit, appetite all recover normal;

Effective: ill temperature of pig body, body colour, spirit, appetite progressively recover normal, and constipation with dry stool degree is progressively improved;

Invalid: ill pig symptom is not alleviated, and even increases the weight of death.

2.2 observed results are as shown in table 1.

Data from table 1: compared with matched group, the treatment of medicine to pig blue-ear disease of the embodiment of the present invention 1 and embodiment 2 has evident in efficacy, and side effect is low, the advantage that treated effect is high; Find in long-term observation subsequently, embodiment group 1 and embodiment group 2 pig of curing again sickness rate is starkly lower than the pig of matched group.

After 2.3 counteracting toxic substances symptom with cut open inspection result

After counteracting toxic substances, the symptom of ill pig is: dyspnea, and sometimes in ventral breathing, loss of appetite or absolutely useless, body temperature is elevated to more than 40 DEG C, diarrhoea.Indivedual pig ear, skin surface are blue.Analyse piglet dead after counteracting toxic substances, find that lung and pleura stick together, lung swelling, lung has dispersivity hemorrhage, in interstitial pneumonia, and some lung atrophys, in carnification, serious loss gas exchange capacity; The cystose mucus of volume or faint yellow purulence mucus is had in trachea-bronchial epithelial cell.

Concrete case

Case 1

Pig farm, Linyi in August, 2012 finds 23 sows suffering from pig blue-ear disease, ill pig occurs that spirit is depressed, poor appetite or useless absolutely, heating, there is dyspnea in various degree, 8 sow in puerperal agalactia in puerperal and vaginal secretions outflow.Use the injection of the embodiment of the present invention 1 to treat, once, each 5mL/ head, uses three days continuously in injection every day.21 sow recoveries from illness, continuation use in two is also fully recovered for two days in addition, and symptom all disappears.

Case 2

Feicheng, Shandong in July, 2012 self-employed entrepreneurs that raises pigs finds that 50 piglets all occur dyspnea, and sometimes in ventral breathing, loss of appetite, serious is even absolutely useless, and body temperature is elevated to more than 40 DEG C, and has the symptom of diarrhoea, finds dead 5 piglets in symptom two days.By dead piglet censorship, find that lung and pleura stick together, lung swelling, lung has dispersivity hemorrhage, in interstitial pneumonia, and some lung atrophys, in carnification, serious loss gas exchange capacity.Be diagnosed as pig blue-ear disease, use the powder of the embodiment of the present invention 2 to treat, powder is mixed in feedstuff, conventionally feed, each mixed volume is 8% of feedstuff gross weight, uses three days continuously, 40 piglets all recover normal, and other 5 continuation uses also all recover normal for two days.

The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.

Claims (4)

1. be used for the treatment of the medicine of pig blue-ear disease, it is characterized in that, described medicine is made up of the raw material of following parts by weight: Ganoderma 20-30 weight portion, Herba of Artemisiae admsii 10-17 weight portion, Radix Scutellariae 10-20 weight portion, chopping board 10-15 weight portion, to pick up the ears 10-20 weight portion, Herba Peperomiae pellucidae 10-20 weight portion, bean pears 10-30 weight portion, Herba Leonuri 15-20 weight portion, Carnis Equi Asini 10-15 weight portion, Bulbus Allii 10-15 weight portion, Fructus Benincasae 10-20 weight portion, Radix Salviae Miltiorrhizae 10-25 weight portion, Fructus Liquidambaris 13-20 weight portion, Radix Isatidis 10-20 weight portion, Radix Glycyrrhizae 10-20 weight portion, Foliumet Ramulus Evodiae 15-25 weight portion, Radix Berberidis 15-25 weight portion and Radix Zanthoxyli 10-20 weight portion.

2. the medicine being used for the treatment of pig blue-ear disease according to claim 1, it is characterized in that, described medicine is made up of the raw material of following parts by weight: Ganoderma 25-30 weight portion, Herba of Artemisiae admsii 12-17 weight portion, Radix Scutellariae 12-18 weight portion, chopping board 10-15 weight portion, to pick up the ears 13-17 weight portion, Herba Peperomiae pellucidae 12-18 weight portion, bean pears 15-25 weight portion, Herba Leonuri 15-20 weight portion, Carnis Equi Asini 10-15 weight portion, Bulbus Allii 10-14 weight portion, Fructus Benincasae 13-17 weight portion, Radix Salviae Miltiorrhizae 13-21 weight portion, Fructus Liquidambaris 15-20 weight portion, Radix Isatidis 12-17 weight portion, Radix Glycyrrhizae 12-18 weight portion, Foliumet Ramulus Evodiae 15-25 weight portion, Radix Berberidis 17-23 weight portion and Radix Zanthoxyli 12-20 weight portion.

3. the medicine being used for the treatment of pig blue-ear disease according to claim 1, it is characterized in that, described medicine is made up of the raw material of following parts by weight: Ganoderma 28 weight portion, Herba of Artemisiae admsii 15 weight portion, Radix Scutellariae 16 weight portion, chopping board 13 weight portion, to pick up the ears 15 weight portions, Herba Peperomiae pellucidae 15 weight portion, bean pears 21 weight portion, Herba Leonuri 18 weight portion, Carnis Equi Asini 13 weight portion, Bulbus Allii 12 weight portion, Fructus Benincasae 15 weight portion, Radix Salviae Miltiorrhizae 17 weight portion, Fructus Liquidambaris 17 weight portion, Radix Isatidis 15 weight portion, Radix Glycyrrhizae 16 weight portion, Foliumet Ramulus Evodiae 20 weight portion, Radix Berberidis 18 weight portion and Radix Zanthoxyli 17 weight portion.

4. the preparation method being used for the treatment of the medicine of pig blue-ear disease as described in any one of claim 1-3, is characterized in that, the dosage form of described medicine is powder, and its preparation method comprises the following steps:

The first step, take the mixing of all raw materials by weight, be ground into powder, load in multi-function extractor, adding relative to the determining alcohol of mixture quality 3 ~ 5 times is the ethanol of 80% ~ 90%, and reflux is extracted for 1 hour ~ 3 hours, obtains extracting solution, steam pressure in reflux process is 0.4MPa ~ 0.6MPa, extracting liquid filtering is obtained the first filtrate and filtering residue;

Second step, adding relative to the determining alcohol of first step mixture quality 1 ~ 3 times by the filtering residue that the first step obtains is the ethanol of 80% ~ 90%, reflux is extracted for 0.5 hour ~ 1.5 hours, obtain extracting solution, steam pressure in reflux process is 0.4MPa ~ 0.6MPa, extracting liquid filtering is obtained the second filtrate;

3rd step, the first filtrate and the second filtrate are merged, concentrating under reduced pressure is except desolventizing, obtain and get dry extract, dry cream is put into spray dryer dry, ensure that inlet temperature is at 170 DEG C ~ 190 DEG C, leaving air temp 60 DEG C ~ 80 DEG C, is ground into 200 order ~ 400 orders subsequently, obtains powder.