CN105213918A - The immune formulation of control Porcine reproductive and respiratory syndrome - Google Patents
The immune formulation of control Porcine reproductive and respiratory syndrome Download PDFInfo
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Abstract
The invention provides a kind of immune formulation for preventing and treating Porcine reproductive and respiratory syndrome, its crude drug comprises Herba Epimedii, Rhizoma Polygonati, Semen Euryales, Herba Ephedrae, Herba Centipedae, Semen Astragali Complanati, Fructus Forsythiae, Radix Campanumoeae, Fructus Gardeniae grandiflorae, Rhizoma Dioscoreae, Cortex Moutan, Radix Et Caulis Acanthopanacis Senticosi, white Rhizoma Curculiginis, Herba Oxalidis strictae, Herba Galii Bungei, Radix et Rhizoma Thalictri, Herba Taxilli, Radix et Rhizoma Rhei Franzenbachii, Radix Adenophorae and Fructus Alpiniae Oxyphyllae.It can pass through body regulating action, the cellular immunization of enhancing body, humoral immunization, immune cell activated, the generation of inducing interferon, impel virus can not in porcine somatic cell survival and reproduction, and play the effect of anti-Porcine reproductive and respiratory syndrome.
Description
Technical field
The present invention relates to immunological technique field, particularly relating to a kind of immune formulation for preventing and treating Porcine reproductive and respiratory syndrome.
Background technology
Porcine reproductive and respiratory syndrome (PorcineReproductiveandRespira-tionSyndrome, PRRS) be a kind of Acute exposure sexually transmitted disease caused by porcine reproductive and respiratory syndrome virus, mainly cause the breeding difficulty of in-pig and the respiratory symptom of piglet, cause the mortality of the breeding difficultys such as in-pig miscarriage, stillborn fetus, mummy tire, weak son and piglet.This disease is a kind of acute, high lethal infectious diseases, and viral pathogenesis power is strong, and spread speed is fast, and swinery M & M is high, and piglet sickness rate can reach 100% mortality rate can reach more than 30%, and Sow abortion rate can reach more than 30%.Growing and fattening pigs, sow also can be fallen ill death.
This disease there is no effective Therapeutic Method so far, and vaccine control is main measure.The research of vaccine obtains remarkable break-throughs, and commercial Porcine reproductive and respiratory syndrome vaccine has inactivated vaccine and attenuated live vaccines two class in the world.The main Problems existing of Porcine reproductive and respiratory syndrome vaccine is clinically: the first, and vaccine produces immunoreation needs the long period; Secondly, Porcine reproductive and respiratory syndrome vaccine does not have protection completely, and especially inactivated vaccine can not activated cell immunity; 3rd, can there is a few time-of-week in Porcine reproductive and respiratory syndrome attenuated vaccine in immune swine soma, and propagate from note Seedling pig to susceptible pig.Therefore, application inactivated vaccine strengthens vaccine immunity effect and safety, and the immune effect that development immunostimulant improves Porcine reproductive and respiratory syndrome inactivated vaccine has significant advantage.
Discovered in recent years Chinese medicine has prevented good effect to viral disease, and therefore development goes out Chinese medicine preparation and holds out broad prospects to prevent and treat this disease.Chinese veterinarian thinks, PRRS belongs to temperature (epidemic disease) sick category, is the disease because pig causes by the dry exopathogen invasion and attack such as cold of abnormally climatic pathogens, summer-heat, has the features such as infectiousness, popularity, high heat.The mechanism of Chinese herb prevention Porcine reproductive and respiratory syndrome has direct effect and indirect action, direct effect be Chinese medicine preparation blocking virus to Normocellular absorption, penetrate, the link such as to copy and reach antiviral object.Indirect action be Chinese medicine by body regulating action, the cellular immunization of enhancing body, humoral immunization, immune cell activated, the generation of inducing interferon, impel virus can not in porcine somatic cell survival and reproduction, and play the effect of anti-Porcine reproductive and respiratory syndrome.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of immune formulation for preventing and treating Porcine reproductive and respiratory syndrome, it can pass through body regulating action, the cellular immunization of enhancing body, humoral immunization, immune cell activated, the generation of inducing interferon, impel virus can not in porcine somatic cell survival and reproduction, and play the effect of anti-Porcine reproductive and respiratory syndrome.
For solving the problems of the technologies described above, the invention provides a kind of immune formulation for preventing and treating Porcine reproductive and respiratory syndrome, its crude drug comprises Herba Epimedii, Rhizoma Polygonati, Semen Euryales, Herba Ephedrae, Herba Centipedae, Semen Astragali Complanati, Fructus Forsythiae, Radix Campanumoeae, Fructus Gardeniae grandiflorae, Rhizoma Dioscoreae, Cortex Moutan, Radix Et Caulis Acanthopanacis Senticosi, white Rhizoma Curculiginis, Herba Oxalidis strictae, Herba Galii Bungei, Radix et Rhizoma Thalictri, Herba Taxilli, Radix et Rhizoma Rhei Franzenbachii, Radix Adenophorae and Fructus Alpiniae Oxyphyllae.
Wherein, in described immune formulation, the weight portion of each crude drug is respectively Herba Epimedii 20 weight portion ~ 30 weight portion, Rhizoma Polygonati 35 weight portion ~ 45 weight portion, Semen Euryales 25 weight portion ~ 35 weight portion, Herba Ephedrae 10 weight portion ~ 20 weight portion, Herba Centipedae 15 weight portion ~ 25 weight portion, Semen Astragali Complanati 20 weight portion ~ 30 weight portion, Fructus Forsythiae 30 weight portion ~ 40 weight portion, Radix Campanumoeae 25 weight portion ~ 35 weight portion, Fructus Gardeniae grandiflorae 15 weight portion ~ 25 weight portion, Rhizoma Dioscoreae 20 weight portion ~ 30 weight portion, Cortex Moutan 15 weight portion ~ 25 weight portion, Radix Et Caulis Acanthopanacis Senticosi 30 weight portion ~ 40 weight portion, white Rhizoma Curculiginis 10 weight portion ~ 20 weight portion, Herba Oxalidis strictae 10 weight portion ~ 20 weight portion, Herba Galii Bungei 15 weight portion ~ 25 weight portion, Radix et Rhizoma Thalictri 10 weight portion ~ 20 weight portion, Herba Taxilli 25 weight portion ~ 35 weight portion, Radix et Rhizoma Rhei Franzenbachii 15 weight portion ~ 25 weight portion, Radix Adenophorae 20 weight portion ~ 30 weight portion and Fructus Alpiniae Oxyphyllae 10 weight portion ~ 20 weight portion.
Wherein, in described immune formulation, the weight portion of each crude drug is respectively Herba Epimedii 23 weight portion ~ 27 weight portion, Rhizoma Polygonati 38 weight portion ~ 42 weight portion, Semen Euryales 28 weight portion ~ 32 weight portion, Herba Ephedrae 13 weight portion ~ 17 weight portion, Herba Centipedae 18 weight portion ~ 22 weight portion, Semen Astragali Complanati 23 weight portion ~ 27 weight portion, Fructus Forsythiae 33 weight portion ~ 37 weight portion, Radix Campanumoeae 28 weight portion ~ 32 weight portion, Fructus Gardeniae grandiflorae 18 weight portion ~ 22 weight portion, Rhizoma Dioscoreae 23 weight portion ~ 27 weight portion, Cortex Moutan 18 weight portion ~ 22 weight portion, Radix Et Caulis Acanthopanacis Senticosi 33 weight portion ~ 37 weight portion, white Rhizoma Curculiginis 13 weight portion ~ 17 weight portion, Herba Oxalidis strictae 13 weight portion ~ 17 weight portion, Herba Galii Bungei 18 weight portion ~ 22 weight portion, Radix et Rhizoma Thalictri 13 weight portion ~ 17 weight portion, Herba Taxilli 28 weight portion ~ 32 weight portion, Radix et Rhizoma Rhei Franzenbachii 18 weight portion ~ 22 weight portion, Radix Adenophorae 23 weight portion ~ 27 weight portion and Fructus Alpiniae Oxyphyllae 13 weight portion ~ 17 weight portion.
Wherein, in described immune formulation, the weight portion of each crude drug is respectively Herba Epimedii 25 weight portion, Rhizoma Polygonati 40 weight portion, Semen Euryales 30 weight portion, Herba Ephedrae 15 weight portion, Herba Centipedae 20 weight portion, Semen Astragali Complanati 25 weight portion, Fructus Forsythiae 35 weight portion, Radix Campanumoeae 30 weight portion, Fructus Gardeniae grandiflorae 20 weight portion, Rhizoma Dioscoreae 25 weight portion, Cortex Moutan 20 weight portion, Radix Et Caulis Acanthopanacis Senticosi 35 weight portion, white Rhizoma Curculiginis 15 weight portion, Herba Oxalidis strictae 15 weight portion, Herba Galii Bungei 20 weight portion, Radix et Rhizoma Thalictri 15 weight portion, Herba Taxilli 30 weight portion, Radix et Rhizoma Rhei Franzenbachii 20 weight portion, Radix Adenophorae 25 weight portion and Fructus Alpiniae Oxyphyllae 15 weight portion.
Present invention also offers the preparation method of above-mentioned Traditional Chinese medicine immune preparation, be specially:
Each crude drug composition is crushed to 20 ~ 40 orders respectively, mixes by above-mentioned weight portion, then put it into volume and be 6 ~ 12 times of medical material amount and volumetric concentration is in the alcoholic solution of 70% ~ 95%, adopt reflux extraction, extract 2 ~ 4 times, each extraction time is 1.5 ~ 2.5h, and Extracting temperature is 65 DEG C ~ 85 DEG C, solution mixing, filtering and collecting filter liquid will be extracted, be placed on Rotary Evaporators concentrated, removing alcohol solvent, dry, pulverize, adaptive immune preparation.
Beneficial effect of the present invention:
The invention provides a kind of immune formulation for preventing and treating Porcine reproductive and respiratory syndrome, it can pass through body regulating action, the cellular immunization of enhancing body, humoral immunization, immune cell activated, the generation of inducing interferon, impel virus can not in porcine somatic cell survival and reproduction, and play the effect of anti-Porcine reproductive and respiratory syndrome.
Detailed description of the invention
Theory of Chinese medical science is thought, though the generation of Porcine reproductive and respiratory syndrome and change intricate, be attributed to 2 points, i.e. body intrinsic factor and the interactional result of extrinsic factor.Traditional Chinese veterinary medicine thinks that this is the result that (healthy energy) struggles against with (pathogen).(healthy energy) is the functional activity of each internal organs organ of poultry body, and the adaptive capacity of its environment to external world, to the resistivity of paathogenic factor, rehabilitation ability, it is the identification foreign body based on the material such as function and qi-blood-body fluid, get rid of foreign body, maintain self homeostasis and stable function.(pathogen) refers to extraneous six climate exopathogens, the gas of epidemic disease, comprises again pathological change that body imbalance of YIN and YANG produces and pathological product etc.Whether the health of body depends on the power of (healthy energy) and (pathogen), from traditional Chinese veterinary medicine angle analysis, belongs to Epidemic Febrile Disease, and its morbidity at pig body changes available three Jiao, and the part of the body cavity above the diaphragm housing the heart and lungs, middle Jiao, the part of the body cavity below the umbilicus, housing the bladder, kidneys and bowels, defensive-qi-nutrient-blood carrys out determination for the treatment of based on pathogenesis obtained through differentiation of symptoms and signs.(exogenous pathogen mutual aid) is predisposing factors, and when the five internal organs that namely traditional Chinese medical science is said are in (void), virus is taken advantage of a weak point, and cause pathogen being excessive but vital QI weak, disease-resistant unable, this disease is multiple for summer.Many because of the damp and hot fire-transformation of diseases caused by exogenous pathogenic factor, cause inhibited pulmonary QI, abnormal ascending-descending of QI, caused by imbalance of YIN and YANG.The immune system of modern medicine has defence, from hidden and supervision 3 large functions.Defence refers to the infection resisting various pathogenic microorganism, and the effect of traditional Chinese veterinary medicine healthy energy defence exopathogen similarly; From hidden just refer to remove autoantigen, get rid of extrinsic factor ten disturb, stablize environment, maintenance immunologic balance; Immune surveillance is in case stop machine somatic mutation is " alien elements ", and pass through immunological rejection, repel these " alien elementses ", traditional Chinese veterinary medicine healthy energy coordinate zang-fu differentiation QI and blood mutual dependence for existence, mutually restriction, remove in evil, re-establish the effect of equilibrium between yin and yang also similarly.It can thus be appreciated that the said carcass healthy energy of traditional Chinese veterinary medicine, has the immune identity function of modern veterinary, and immune organ, tissue, cell may be exactly the material foundation of tcm of healthy energy, the process of struggle between vital QI and pathogen is exactly immunoreactive process.Therefore, the generation of whole disease, development and change, be all reaction of " healthy energy " and " pathogen " struggle mutually, good and evil struggle runs through disease progression all the time, therefore, in line with strengthening vital QI to eliminate pathogenic factors in treatment, the principle of giving consideration to both the incidental and fundamental.
Based on this, the invention provides a kind of immune formulation for preventing and treating Porcine reproductive and respiratory syndrome, its crude drug comprises Herba Epimedii, Rhizoma Polygonati, Semen Euryales, Herba Ephedrae, Herba Centipedae, Semen Astragali Complanati, Fructus Forsythiae, Radix Campanumoeae, Fructus Gardeniae grandiflorae, Rhizoma Dioscoreae, Cortex Moutan, Radix Et Caulis Acanthopanacis Senticosi, white Rhizoma Curculiginis, Herba Oxalidis strictae, Herba Galii Bungei, Radix et Rhizoma Thalictri, Herba Taxilli, Radix et Rhizoma Rhei Franzenbachii, Radix Adenophorae and Fructus Alpiniae Oxyphyllae.
Further preferably, described immune formulation is only prepared from by above-mentioned raw materials medicine.
In described immune formulation, the weight portion of each crude drug is respectively Herba Epimedii 20 weight portion ~ 30 weight portion, Rhizoma Polygonati 35 weight portion ~ 45 weight portion, Semen Euryales 25 weight portion ~ 35 weight portion, Herba Ephedrae 10 weight portion ~ 20 weight portion, Herba Centipedae 15 weight portion ~ 25 weight portion, Semen Astragali Complanati 20 weight portion ~ 30 weight portion, Fructus Forsythiae 30 weight portion ~ 40 weight portion, Radix Campanumoeae 25 weight portion ~ 35 weight portion, Fructus Gardeniae grandiflorae 15 weight portion ~ 25 weight portion, Rhizoma Dioscoreae 20 weight portion ~ 30 weight portion, Cortex Moutan 15 weight portion ~ 25 weight portion, Radix Et Caulis Acanthopanacis Senticosi 30 weight portion ~ 40 weight portion, white Rhizoma Curculiginis 10 weight portion ~ 20 weight portion, Herba Oxalidis strictae 10 weight portion ~ 20 weight portion, Herba Galii Bungei 15 weight portion ~ 25 weight portion, Radix et Rhizoma Thalictri 10 weight portion ~ 20 weight portion, Herba Taxilli 25 weight portion ~ 35 weight portion, Radix et Rhizoma Rhei Franzenbachii 15 weight portion ~ 25 weight portion, Radix Adenophorae 20 weight portion ~ 30 weight portion and Fructus Alpiniae Oxyphyllae 10 weight portion ~ 20 weight portion.
Further preferably, in described immune formulation, the weight portion of each crude drug is respectively Herba Epimedii 23 weight portion ~ 27 weight portion, Rhizoma Polygonati 38 weight portion ~ 42 weight portion, Semen Euryales 28 weight portion ~ 32 weight portion, Herba Ephedrae 13 weight portion ~ 17 weight portion, Herba Centipedae 18 weight portion ~ 22 weight portion, Semen Astragali Complanati 23 weight portion ~ 27 weight portion, Fructus Forsythiae 33 weight portion ~ 37 weight portion, Radix Campanumoeae 28 weight portion ~ 32 weight portion, Fructus Gardeniae grandiflorae 18 weight portion ~ 22 weight portion, Rhizoma Dioscoreae 23 weight portion ~ 27 weight portion, Cortex Moutan 18 weight portion ~ 22 weight portion, Radix Et Caulis Acanthopanacis Senticosi 33 weight portion ~ 37 weight portion, white Rhizoma Curculiginis 13 weight portion ~ 17 weight portion, Herba Oxalidis strictae 13 weight portion ~ 17 weight portion, Herba Galii Bungei 18 weight portion ~ 22 weight portion, Radix et Rhizoma Thalictri 13 weight portion ~ 17 weight portion, Herba Taxilli 28 weight portion ~ 32 weight portion, Radix et Rhizoma Rhei Franzenbachii 18 weight portion ~ 22 weight portion, Radix Adenophorae 23 weight portion ~ 27 weight portion and Fructus Alpiniae Oxyphyllae 13 weight portion ~ 17 weight portion.
Most preferably, in described immune formulation, the weight portion of each crude drug is respectively Herba Epimedii 25 weight portion, Rhizoma Polygonati 40 weight portion, Semen Euryales 30 weight portion, Herba Ephedrae 15 weight portion, Herba Centipedae 20 weight portion, Semen Astragali Complanati 25 weight portion, Fructus Forsythiae 35 weight portion, Radix Campanumoeae 30 weight portion, Fructus Gardeniae grandiflorae 20 weight portion, Rhizoma Dioscoreae 25 weight portion, Cortex Moutan 20 weight portion, Radix Et Caulis Acanthopanacis Senticosi 35 weight portion, white Rhizoma Curculiginis 15 weight portion, Herba Oxalidis strictae 15 weight portion, Herba Galii Bungei 20 weight portion, Radix et Rhizoma Thalictri 15 weight portion, Herba Taxilli 30 weight portion, Radix et Rhizoma Rhei Franzenbachii 20 weight portion, Radix Adenophorae 25 weight portion and Fructus Alpiniae Oxyphyllae 15 weight portion.
Each crude drug pharmacology is as follows:
Herba Epimedii: latin name EpimediiFolium, acrid in the mouth, sweet, warm in nature, enter liver, kidney channel, there is kidney-replenishing, bone and muscle strengthening, effect of wind-damp dispelling, cure mainly that impotence due to deficiency of the kidney, seminal emission premature ejaculation, waist knee joint flaccidity are soft, cold extremities with aversion to cold; The cards such as arthralgia due to cold-dampnes or spasm of the limbs numbness.
Rhizoma Polygonati: latin name PolygonatiRhizoma, sweet in the mouth, property is put down, enter lung, spleen, kidney channel, there is enriching qi and yin, spleen invigorating lung moistening, effect of enriching kidney essence, for taste deficiency of qi and yin, lack of appetite is unable, lung-dryness syndrome due to deficiency of YIN, the few expectorant of dry cough, quenching one's thirst of yin asthenia generating intrinsic heat, the card such as deficiency of kidney essence, impotence and seminal emission, dizziness and tinnitus, blurred vision dim eyesight.
Semen Euryales: sweet in the mouth, puckery, property is put down.Return spleen, kidney channel.Have supplementing the kidney to control the nocturnal, spleen reinforcing antidiarrheal, the function of damp eliminating leukorrhagia stopping, cures mainly emission, diarrhea due to hypofunction of the spleen, and insufficiency of the spleen nebulousurine is with inferior card.
Herba Ephedrae: acrid in the mouth, micro-hardship, warm in nature, enter lung, urinary bladder channel, have and send out cold inducing sweat, depressed lung-energy dispersing, the effect of inducing diuresis to remove edema, is mainly used in the exterior excess syndromes such as the fever without perspiration of affection of exogenous wind-cold; Rheumatic arthralgia pain; Pathogenic cold attacking the lung or accumulation of heat in the lung to cough with asthma QI rising in reverse order, edema, the cards such as dysuria.
Herba Centipedae: acrid in the mouth, warm in nature, enter lung, Liver Channel, there is cold-dispelling, clearing the nasal passage, eliminating phlegm and stopping cough, the effect of dissipating blood stasis for subsidence of swelling, for exterior syndrome due to wind-cold, nasal obstruction watery nasal discharge and nasal sinusitis, cough with copious phlegm, pertussis, the cards such as sore, carbuncle and painful swelling.
Semen Astragali Complanati: warm in nature, sweet in the mouth, has temperature compensation Liver and kidney, controlling nocturnal emission with astringent drugs, reducing urination, the effect of improving eyesight, for impotence due to deficiency of the kidney, seminal emission premature ejaculation, frequent micturition, or leukorrhagia, soreness of the waist and knees, lumbago; Deficiency of the liver and kindey, blurred vision order is dark, the cards such as visual deterioration.
Fructus Forsythiae: bitter in the mouth, cold nature, enters the heart, liver, gallbladder, small intestine meridian, has heat-clearing and toxic substances removing, effect of dispersing swelling and dissipating binds, for carbuncle, scrofula, acute mastitis, erysipelas, anemopyretic cold, epidemic febrile disease from the beginning of, warmly to join the army, high hot excessive thirst, the cards such as coma sends out speckle, pyretic stranguria urine retention.
Radix Campanumoeae: latin name radixcampanumoeajavanicae is the root of campanulaceae leopard platymiscium Campanumoea javanica and leopard, sweet in the mouth, property is put down, and returns lung, spleen channel, has invigorating the spleen and benefiting QI, tonifying the lung and stopping cough, effect of stimulating milk secretion, cure mainly the empty internal injury of labor, the deficiency of vital energy is weak, cardiopalmus, hyperhidrosis, diarrhea due to hypofunction of the spleen, leucorrhea, milk is rare, the cards such as insufficiency of the spleen cough.
Fructus Gardeniae grandiflorae: be the fruit of Rubiaceae Gardenia Ellis plant Flos Caryophylli Fructus Gardeniae, bitter in the mouth, cold in nature, have heat-clearing and toxic substances removing, effect of reducing swelling and alleviating pain, cures mainly pyretic toxicity, jaundice, oedema due to nephritis, dampens cards such as spraining.
Rhizoma Dioscoreae: latin name dioscoreaerhizoma, sweet in the mouth, property are flat, and enter lung, spleen, stomach warp, have spleen reinforcing nourishing the stomach, lung benefiting reinforces the kidney, and the effect of YIN nourishing and the production of body fluid promoting, cures mainly weakness of the spleen and stomach, the lack of appetite poor appetite of dysfunction of the spleen in transportation, the cards such as the vexed diarrhoea of gastral cavity.
Cortex Moutan: latin name MoutanCortex, bitter in the mouth, pungent, cold nature, enters the heart, liver, kidney channel, has the effect of clearing away heat and cooling blood, blood circulation promoting and blood stasis dispelling, for the card such as fever due to yin deficiency, the acute appendicitis of damp and hot knot poison, depressed blood stasis, xerostomia, deep red tongue of excessive noxious heat.
Radix Et Caulis Acanthopanacis Senticosi: be the root of studies on plants of Acanthopanax Miq. in Araliaceae Radix Et Caulis Acanthopanacis Senticosi, rhizome or stem and leaf, latin name AcanthopanacisSenticosiRadixetRhizomaseuCaulis, acrid in the mouth, hardship, micro-sweet, warm in nature, enter spleen, kidney, heart channel, there is replenishing QI to invigorate the spleen, tonifying the kidney for tranquilization, effect of expelling wind and removing dampness, soft for anemofrigid-damp arthralgia, lumbocrural pain, muscles and bones flaccidity, be slow in action, the card such as body weakness with emaciation is weak, traumatic injury, fracture, edema, beriberi, uncontrolled urination are itched.
White Rhizoma Curculiginis: be the root of the large Stichopus variegatus (Sempen) of Dipsacaceae thorn Genus Dipsacus plant, sweet in the mouth, micro-hardship, warm in nature, there is lung benefiting spleen invigorating, kidney invigorating and YANG supporting, effect of relaxing muscles and tendons to promote blood circulation, cures mainly cough due to deficiency of the lung, insufficiency of the spleen dyspepsia, impotence due to deficiency of the kidney, leukorrhagia, uterine prolapse, traumatic injury, the cards such as fracture.
Herba Oxalidis strictae: be the branch and leaf of Oleaceae jasminum plant Herba Oxalidis strictae, mildly bitter flavor, cool in nature, have heat clearing away, dampness removing, effect of removing toxic substances, cures mainly damp-heat dysentery, stomachache tenesmus, beriberoid pyretic arthralgia, the cards such as extremity swell and ache, skin ulcer scabies.
Herba Galii Bungei: be the herb of Rubiaceae clivers four leaf humulus, sweet in the mouth, hardship, property is put down, and have heat-clearing and toxic substances removing, effect of inducing diuresis to remove edema, cures mainly pyretic stranguria, dysentery, hemoptysis, leucorrhea with red and white discharge, infantile malnutrition, traumatic injury, the cards such as venom.
Radix et Rhizoma Thalictri: be the root and rhizome of Ranunculaceae thalictrum spp spun gold Radix et Rhizoma Thalictri, Zhaotong Thalictrum aquilegifolium L. var. sibiricum Regel, plateau Thalictrum aquilegifolium L. var. sibiricum Regel, leafy Thalictrum aquilegifolium L. var. sibiricum Regel, bitter in the mouth, cold in nature, GUIXIN, liver, large intestine channel, there is heat clearing and damp drying, effect of eliminating fire and detoxication, cures mainly diarrhea due to damp-heat, dysentery, jaundice, cold, fever, measles, the cards such as conjunctival congestion and swelling pain.
Herba Taxilli: bitter in the mouth, sweet, property is put down, and enters liver, kidney channel, have the effect of invigorating the liver and kidney, bone and muscle strengthening, wind-damp dispelling, antiabortive unit, for the soreness of waist pain caused by deficiency of the liver and kindey, muscles and bones flaccidity is soft, the foot and knee being feeble, deficiency of the liver and kindey, essence and blood void is lost, the frequent fetal movement of liability to abortion, the cards such as waist abdomen pain.
Radix et Rhizoma Rhei Franzenbachii: latin name RadixetRhizomeRheiFranzenbachii is the root and rhizome of polygonaceae plant rheum rhabarbarum, bitter in the mouth; Cold in nature, return stomach; Large intestine channel, has removing toxic substances of purging heat; Effect of removing heat from blood clots absorbing, cures mainly jaundice due to damp-heat; Dysentery; Amenorrhea is suffered from abdominal pain; Hematemesis and epistaxis; Traumatic injury stasis of blood pain; Carbuncle furunculosis; Aphthous stomatitis; The cards such as burn and scald.
Radix Adenophorae: sweet in the mouth, micro-hardship, cold nature, enter lung, stomach warp, there is nourishing the lung to arrest cough, the effect of nourishing the stomach to promote the production of body fluid, be mainly used in lung-dryness syndrome due to deficiency of YIN, dry cough few expectorant, chronic cough expectorant blood, the cards such as the few tongue of deficiency of stomach-Yin, mouthfeel parched throat, constipation, red tongue, cellular immunization and non-specific immunity can be improved, suppress humoral immunization, there is the function of immunity moderation balance.
Fructus Alpiniae Oxyphyllae: acrid in the mouth, warm in nature, return spleen, kidney channel, have warming the kidney controlling nocturnal emission with astringent drugs reducing urination, warming spleen and stopping diarrha effect taking the photograph saliva, cures mainly enuresis due to deficiency of the kidney, frequent micturition, and seminal emission nebulousurine, spleen-cold syndrome is had loose bowels, effect of cold type of pain in abdomen.
Present invention also offers the preparation method of above-mentioned Traditional Chinese medicine immune preparation, be specially:
Each crude drug composition is crushed to 20 ~ 40 orders respectively, mixes by above-mentioned weight portion, then put it into volume and be 6 ~ 12 times of medical material amount and volumetric concentration is in the alcoholic solution of 70% ~ 95%, adopt reflux extraction, extract 2 ~ 4 times, each extraction time is 1.5 ~ 2.5h, and Extracting temperature is 65 DEG C ~ 85 DEG C, solution mixing, filtering and collecting filter liquid will be extracted, be placed on Rotary Evaporators concentrated, removing alcohol solvent, dry, pulverize, adaptive immune preparation.
Present invention also offers a kind of feedstuff for preventing and treating Porcine reproductive and respiratory syndrome, its raw material comprises above-mentioned immune formulation, Semen Maydis, bean cake, fish flour, calcium hydrogen phosphate, Semen Tritici aestivi, Sal, composite additive of trace elements, compound vitamin additive, lysine and methionine.
Further preferably, described feedstuff is only prepared from by above-mentioned raw materials.
In described raw material, each weight portion is respectively above-mentioned immune formulation 2 weight portion ~ 4 weight portion, Semen Maydis 50 weight portion ~ 60 weight portion, bean cake 8 weight portion ~ 12 weight portion, fish flour 5 weight portion ~ 10 weight portion, calcium hydrogen phosphate 1 weight portion ~ 2 weight portion, Semen Tritici aestivi 10 weight portion ~ 16 weight portion, Sal 0.5 weight portion ~ 1 weight portion, composite additive of trace elements 0.1 weight portion ~ 0.3 weight portion, compound vitamin additive 0.3 weight portion ~ 0.6 weight portion, lysine 0.05 weight portion ~ 0.1 weight portion, methionine 0.05 weight portion ~ 0.1 weight portion.
Further preferably, in described raw material, each weight portion is respectively above-mentioned immune formulation 3 weight portion, Semen Maydis 55 weight portion, bean cake 10 weight portion, fish flour 8 weight portion, calcium hydrogen phosphate 2 weight portion, Semen Tritici aestivi 13 weight portion, Sal 1 weight portion, composite additive of trace elements 0.2 weight portion, compound vitamin additive 0.5 weight portion, lysine 0.1 weight portion, methionine 0.1 weight portion.
Present invention also offers the preparation method of above-mentioned feedstuff, specifically comprise:
The first step, prepares described Traditional Chinese medicine immune preparation;
Second step, the immune formulation first step obtained and calcium hydrogen phosphate, Sal, composite additive of trace elements, compound vitamin additive, lysine and methionine are by above-mentioned weight Homogeneous phase mixing, incorporation time is 2 ~ 3 minutes, diameter 1 ~ 3mm granule is made by granulator, vacuum drying at 45 ~ 55 DEG C of granulating after mixing;
3rd step, Semen Maydis, bean cake, fish flour, Semen Tritici aestivi are pulverized after taking out mix homogeneously according to above-mentioned weight, mixed by the raw material crushed, incorporation time is 4 ~ 5 minutes, diameter 1 ~ 3mm granule is made, then vacuum drying at 45 ~ 55 DEG C by granulator;
4th step, the granule that the granule made by second step and the 3rd step are made mixes, and incorporation time is 4 ~ 5 minutes, obtains particulate material finished product.
Described compound vitamin additive can adopt commercially available any compound vitamin additive, the excellent dimension element that preferred Beijing You Libao Bioisystech Co., Ltd produces.
Described composite additive of trace elements can adopt commercially available any composite additive of trace elements, and the excellent profit that preferred Beijing You Libao Bioisystech Co., Ltd produces is protected.
Below adopt embodiment to describe embodiments of the present invention in detail, to the present invention, how application technology means solve technical problem whereby, and the implementation procedure reaching technique effect can fully understand and implement according to this.
Embodiment 1 immune formulation 1
By Herba Epimedii 25g, Rhizoma Polygonati 40g, Semen Euryales 30g, Herba Ephedrae 15g, Herba Centipedae 20g, Semen Astragali Complanati 25g, Fructus Forsythiae 35g, Radix Campanumoeae 30g, Fructus Gardeniae grandiflorae 20g, Rhizoma Dioscoreae 25g, Cortex Moutan 20g, Radix Et Caulis Acanthopanacis Senticosi 35g, white Rhizoma Curculiginis 15g, Herba Oxalidis strictae 15g, Herba Galii Bungei 20g, Radix et Rhizoma Thalictri 15g, Herba Taxilli 30g, Radix et Rhizoma Rhei Franzenbachii 20g, Radix Adenophorae 25g and Fructus Alpiniae Oxyphyllae 15g is crushed to 40 orders respectively, mix, then put it into volume and be 10 times of medical material amount and volumetric concentration is in the alcoholic solution of 90%, adopt reflux extraction, extract 3 times, each extraction time is 2h, Extracting temperature is 75 DEG C, solution mixing will be extracted, filtering and collecting filter liquid, be placed on Rotary Evaporators concentrated, removing alcohol solvent, dry, pulverize, adaptive immune preparation.
Embodiment 2 immune formulation 2
By Herba Epimedii 23g, Rhizoma Polygonati 38g, Semen Euryales 28g, Herba Ephedrae 13g, Herba Centipedae 18g, Semen Astragali Complanati 23g, Fructus Forsythiae 33g, Radix Campanumoeae 28g, Fructus Gardeniae grandiflorae 18g, Rhizoma Dioscoreae 23g, Cortex Moutan 18g, Radix Et Caulis Acanthopanacis Senticosi 33g, white Rhizoma Curculiginis 13g, Herba Oxalidis strictae 13g, Herba Galii Bungei 18g, Radix et Rhizoma Thalictri 13g, Herba Taxilli 28g, Radix et Rhizoma Rhei Franzenbachii 18g, Radix Adenophorae 23g and Fructus Alpiniae Oxyphyllae 13g is crushed to 40 orders respectively, mix, then put it into volume and be 10 times of medical material amount and volumetric concentration is in the alcoholic solution of 90%, adopt reflux extraction, extract 3 times, each extraction time is 2h, Extracting temperature is 75 DEG C, solution mixing will be extracted, filtering and collecting filter liquid, be placed on Rotary Evaporators concentrated, removing alcohol solvent, dry, pulverize, adaptive immune preparation.
Embodiment 3 immune formulation 3
By Herba Epimedii 27g, Rhizoma Polygonati 42g, Semen Euryales 32g, Herba Ephedrae 17g, Herba Centipedae 22g, Semen Astragali Complanati 27g, Fructus Forsythiae 37g, Radix Campanumoeae 32g, Fructus Gardeniae grandiflorae 22g, Rhizoma Dioscoreae 27g, Cortex Moutan 22g, Radix Et Caulis Acanthopanacis Senticosi 37g, white Rhizoma Curculiginis 17g, Herba Oxalidis strictae 17g, Herba Galii Bungei 22g, Radix et Rhizoma Thalictri 17g, Herba Taxilli 32g, Radix et Rhizoma Rhei Franzenbachii 22g, Radix Adenophorae 27g and Fructus Alpiniae Oxyphyllae 17g is crushed to 40 orders respectively, mix, then put it into volume and be 10 times of medical material amount and volumetric concentration is in the alcoholic solution of 90%, adopt reflux extraction, extract 3 times, each extraction time is 2h, Extracting temperature is 75 DEG C, solution mixing will be extracted, filtering and collecting filter liquid, be placed on Rotary Evaporators concentrated, removing alcohol solvent, dry, pulverize, adaptive immune preparation.
Embodiment 4 feedstuff
Prepared by embodiment 1 immune formulation 30g and calcium hydrogen phosphate 20g, Sal 10g, the excellent profit of composite additive of trace elements protects 2g, the plain 5g of the excellent dimension of compound vitamin additive, lysine 1g and methionine 1g Homogeneous phase mixing, incorporation time is 3 minutes, diameter 2mm granule is made by granulator after mixing, granulation vacuum drying at 50 DEG C, by Semen Maydis 550g, bean cake 100g, fish flour 80g, pulverize after Semen Tritici aestivi 130g mix homogeneously, the raw material crushed is mixed, incorporation time is 5 minutes, diameter 2mm granule is made by granulator, then vacuum drying at 50 DEG C, the granule made above is mixed, incorporation time is 5 minutes, obtain particulate material finished product.
The external virus impact of embodiment 5
Main agents and instrument
Cell growth medium, low sugar DMEM, GIBCO product, add 10% newborn calf serum (the biological company limited of Qingdao Ilex purpurea Hassk.[I.chinensis Sims), every 100ml nutritional solution adds penicillin and the streptomycin of the 10000IU of 1ml.Cell maintenance medium, except newborn calf serum is except 2%, all the other same cell growth mediums.MTT, 3-(4,6-dimethylthiazole-2-xl)-2,5-ethylbenzenes-Thiazolyl blue tetrazolium bromide salt, Amresco Products; Dimethyl sulfoxide (DMSO), analytical pure, Shanghai Ling Feng chemical reagent company limited product; DG3022 type enzyme-linked immunosorbent assay instrument, state-run East China Electronics Co., Ltd pipe factory product.
Medicine prepares:
Immune formulation prepared by the embodiment of the present invention 1 is configured to 60mg/ml, and 0.22 μm of filtering with microporous membrane is degerming.Measuring the maximum safe concentration of Marc-145 cell through trial test is 6.0mg/ml, and be diluted to 3000 μ g/ml with cell maintenance medium, 4 DEG C save backup.
Virus prepares
Porcine reproductive and respiratory syndrome virus, this laboratory-20 DEG C preservation, before use 37 DEG C thaw after, be diluted to 100TCID with cell maintenance medium
50for subsequent use.
Test method
96 orifice plates are inoculated Marc-145 cell, cultivates after adopting first dosing respectively after 24h forms monolayer and add virus, add medicine after first adding virus, after medicine and virus incubation, add three kinds of modes.Namely first add medicine, after hatching 2h, add virus liquid; First add virus, remove virus liquid after hatching 2h and add medicine again; Cell is joined after medicine and virus incubation 2h.Continue to cultivate 72h, measure each hole OD by MTT absorption process enzyme-linked immunosorbent assay instrument
570value, as the index of medicine antagonism virus infected cell.OD value is larger, and antiviral effect is better.
Date processing
Measurement data adopts
represent, compare between group and adopt χ
2inspection, all data all adopt the process of SPSS12.0 statistical software.
Result
The effect of immune formulation opposing porcine reproductive and respiratory syndrome virus infection cell, the results are shown in Table 1.
Effect (the OD of table 1 immune formulation opposing porcine reproductive and respiratory syndrome virus infection cell
570value)
| Drug level (μ g/ml) | Virus after first medicine | First medicine after virus | Add after virus and virus incubation |
| 3000 | 0.597±0.051 b | 0.580±0.021 b | 0.591±0.035 b |
| Virus control | 0.311±0.039 a | 0.299±0.040 a | 0.308±0.049 a |
| Cell controls | 0.619±0.033 | 0.592±0.046 | 0.601±0.043 |
Note: compare with cell controls group,
ap < 0.01, compares with virus control group,
bp < 0.01
Table 1 shows, and three kinds of dosing methods all show, and adopts immune formulation provided by the invention to have the effect of stronger opposing virus infected cell, stronger inhibitory action is had to Porcine reproductive and respiratory syndrome, compared with virus control group, significant difference, P < 0.01.
Embodiment 6 curative effect situation
Experimental group test feed: feedstuff 1 prepared by embodiment 4.
Matched group medicine: cefotaxime sodium, 1g/ props up, and Guangdong Jin Di pharmaceutcal corporation, Ltd produces.
Experimental animal
Case source and clinical examination
In JIUYUE, 2013, the nursery pig on multiple pig farms, ground such as somewhere, Qingdao, the situations such as cluster generation dyspnea (ventral breathing) in various degree, spirit are depressed, drowsiness, inappetence, severe patient cough, asthma, respiratory frequency up to 80 times/more than min, the symptoms such as ear, abdominal part and leg cyanosis.Cut open the visible pulmonary congestion of the sick pig of inspection, occur edema, focal pneumonia; What have has the symptom such as hydrothorax, enlargement of hilar lymph nodes.Once used multiple Drug therapy as sulfadiazine clinically, mycillin etc. are showed no effect, and swinery carried out the immunity of swine fever, pig blue-ear disease and streptococcicosis.
Lab testing
Random sterile blood sampling 10 parts, separation of serum, RT-PCR with reference to the method PRRSV of soup scape unit document detects (Tang Jingyuan, Jiang Ping, Deng Yuxiu, etc., the mixed infection [J] of porcine reproductive and respiratory syndrome virus and pathogenic salmonella, veterinary of China is learned, and 2005 (7): 337-338).
The extraction of virus total RNA
Get serum 0.25ml and add 1mlTrizol reagent, incubated at room 5min, add 0.2ml chloroform, fully rock centrifuge tube 15s, incubated at room 2-3min, 4 DEG C, the centrifugal 15min of 12000r/min, turn with supernatant in another new 1.5ml centrifuge tube, add 0.5ml isopropyl alcohol, fully rock, place 10min, 4 DEG C, the centrifugal 10min of 12000r/min, precipitate nucleic acids, abandon supernatant, add 75% ethanol 1ml, jolt, washing nucleic acid, 4 DEG C, the centrifugal 5min of 8000r/min, abandon supernatant, drying at room temperature precipitation 5min, add 40 μ lDEPC water dissolution precipitate.
Reverse transcription
Get downstream primer GP5.20.5 μ l, 10mmol/ldNTPs0.25 μ l, 5 × reverse transcription buffer2.0 μ l and total serum IgE 7.0 μ l, 65 DEG C of water-bath 10min, then add M-MLV reverse transcription 0.25 μ l, mend to cumulative volume 10.0 μ l, 37 DEG C of water-bath 1h with aseptic double-distilled water.
PCR
Reaction cumulative volume 25 μ l.Forward primer GP5.10.5 μ l, downstream primer GP5.20.5 μ l, 2.5mmol/ldNTPs2.0 μ l, 10 × Mg
2+freebuffer2.5 μ l, 2.5mmol/lMg
2+1.5 μ l, rTap enzyme 2.5U, CDNA1 μ l, mend to cumulative volume 25 μ l with sterilizing distilled water.Reaction cycle parameter, 94 DEG C of denaturation 5min; 94 DEG C of degeneration 45s, 59 DEG C of annealing 45s, 72 DEG C extend 60s, carry out 35 circulations altogether; Then 72 DEG C extend 10min.Get PCR primer and carry out 2% agarose gel electrophoresis.
RT-PCR testing result
10 parts of blood serum sample results are all positive, and according to clinical symptoms, cut open inspection change and results of serological detection, being diagnosed as main pathogen is porcine reproductive and respiratory syndrome virus.
GP TH
Get 600 sick pigs and be divided into 3 groups, put into 3 respectively and clean up and the isolating pig house of sterilizing.Immune formulation feedstuff group 200, the feedstuff adopting the embodiment of the present invention 4 to provide carries out feeding; Antibiotic matched group 200, intramuscular injection every day every cefotaxime sodium 1, adopt ordinary maize feedstuff feeding simultaneously, be used in conjunction 7 days, blank group 200 does not give any medicine, feeding ordinary maize feedstuff.Three groups of equal feedings 7 days.
Efficacy determination
Observe the spirit of pig, search for food, body temperature, breathing, skin and the situation such as ear color and death, judge curative effect by following standard.
Cure: breathe steadily after medication, spirit, diet, body temperature, skin and ear color recover normal.
Take a turn for the better: after medication, symptom obviously alleviates, breathing, spirit, body temperature, diet take a turn for the better.
Invalid: after medication, symptom does not alleviate, even increase the weight of or death.
Total effective rate=cure rate+improvement rate.
Result
Three groups of comparitive study, the results are shown in Table 2.
Table 2 three groups of comparitive study
| Group | Cure number | Cure rate | Good revolution | Improvement rate | Invalid number | Total effective rate |
| Immune formulation | 178 | 89.0％ | 12 | 6.0％ | 10 | 95.0％ |
| Antibiotic | 85 | 42.5％ | 63 | 31.5％ | 52 | 74.0％ |
| Blank | 0 | 0.0％ | 25 | 12.5％ | 175 | 12.5％ |
As can be seen from Table 2, adopt feedstuff provided by the invention, comparatively antibiotic more can well be treated, two groups of significant differences.
All above-mentioned this intellectual properties of primary enforcement, not setting restriction this new product of other forms of enforcement and/or new method.Those skilled in the art will utilize this important information, and foregoing is revised, to realize similar implementation status.But all modifications or transformation belong to the right of reservation based on new product of the present invention.
The above is only preferred embodiment of the present invention, and be not restriction the present invention being made to other form, any those skilled in the art may utilize the technology contents of above-mentioned announcement to be changed or be modified as the Equivalent embodiments of equivalent variations.But everyly do not depart from technical solution of the present invention content, any simple modification, equivalent variations and the remodeling done above embodiment according to technical spirit of the present invention, still belong to the protection domain of technical solution of the present invention.
Claims (5)
1. for preventing and treating an immune formulation for Porcine reproductive and respiratory syndrome, it is characterized in that: crude drug comprises Herba Epimedii, Rhizoma Polygonati, Semen Euryales, Herba Ephedrae, Herba Centipedae, Semen Astragali Complanati, Fructus Forsythiae, Radix Campanumoeae, Fructus Gardeniae grandiflorae, Rhizoma Dioscoreae, Cortex Moutan, Radix Et Caulis Acanthopanacis Senticosi, white Rhizoma Curculiginis, Herba Oxalidis strictae, Herba Galii Bungei, Radix et Rhizoma Thalictri, Herba Taxilli, Radix et Rhizoma Rhei Franzenbachii, Radix Adenophorae and Fructus Alpiniae Oxyphyllae.
2. as claimed in claim 1 for preventing and treating the immune formulation of Porcine reproductive and respiratory syndrome, it is characterized in that: the weight portion of each crude drug is respectively Herba Epimedii 20 weight portion ~ 30 weight portion, Rhizoma Polygonati 35 weight portion ~ 45 weight portion, Semen Euryales 25 weight portion ~ 35 weight portion, Herba Ephedrae 10 weight portion ~ 20 weight portion, Herba Centipedae 15 weight portion ~ 25 weight portion, Semen Astragali Complanati 20 weight portion ~ 30 weight portion, Fructus Forsythiae 30 weight portion ~ 40 weight portion, Radix Campanumoeae 25 weight portion ~ 35 weight portion, Fructus Gardeniae grandiflorae 15 weight portion ~ 25 weight portion, Rhizoma Dioscoreae 20 weight portion ~ 30 weight portion, Cortex Moutan 15 weight portion ~ 25 weight portion, Radix Et Caulis Acanthopanacis Senticosi 30 weight portion ~ 40 weight portion, white Rhizoma Curculiginis 10 weight portion ~ 20 weight portion, Herba Oxalidis strictae 10 weight portion ~ 20 weight portion, Herba Galii Bungei 15 weight portion ~ 25 weight portion, Radix et Rhizoma Thalictri 10 weight portion ~ 20 weight portion, Herba Taxilli 25 weight portion ~ 35 weight portion, Radix et Rhizoma Rhei Franzenbachii 15 weight portion ~ 25 weight portion, Radix Adenophorae 20 weight portion ~ 30 weight portion and Fructus Alpiniae Oxyphyllae 10 weight portion ~ 20 weight portion.
3. as claimed in claim 1 or 2 for preventing and treating the immune formulation of Porcine reproductive and respiratory syndrome, it is characterized in that: the weight portion of each crude drug is respectively Herba Epimedii 23 weight portion ~ 27 weight portion, Rhizoma Polygonati 38 weight portion ~ 42 weight portion, Semen Euryales 28 weight portion ~ 32 weight portion, Herba Ephedrae 13 weight portion ~ 17 weight portion, Herba Centipedae 18 weight portion ~ 22 weight portion, Semen Astragali Complanati 23 weight portion ~ 27 weight portion, Fructus Forsythiae 33 weight portion ~ 37 weight portion, Radix Campanumoeae 28 weight portion ~ 32 weight portion, Fructus Gardeniae grandiflorae 18 weight portion ~ 22 weight portion, Rhizoma Dioscoreae 23 weight portion ~ 27 weight portion, Cortex Moutan 18 weight portion ~ 22 weight portion, Radix Et Caulis Acanthopanacis Senticosi 33 weight portion ~ 37 weight portion, white Rhizoma Curculiginis 13 weight portion ~ 17 weight portion, Herba Oxalidis strictae 13 weight portion ~ 17 weight portion, Herba Galii Bungei 18 weight portion ~ 22 weight portion, Radix et Rhizoma Thalictri 13 weight portion ~ 17 weight portion, Herba Taxilli 28 weight portion ~ 32 weight portion, Radix et Rhizoma Rhei Franzenbachii 18 weight portion ~ 22 weight portion, Radix Adenophorae 23 weight portion ~ 27 weight portion and Fructus Alpiniae Oxyphyllae 13 weight portion ~ 17 weight portion.
4. the immune formulation for preventing and treating Porcine reproductive and respiratory syndrome as described in claims 1 to 3, it is characterized in that: the weight portion of each crude drug is respectively Herba Epimedii 25 weight portion, Rhizoma Polygonati 40 weight portion, Semen Euryales 30 weight portion, Herba Ephedrae 15 weight portion, Herba Centipedae 20 weight portion, Semen Astragali Complanati 25 weight portion, Fructus Forsythiae 35 weight portion, Radix Campanumoeae 30 weight portion, Fructus Gardeniae grandiflorae 20 weight portion, Rhizoma Dioscoreae 25 weight portion, Cortex Moutan 20 weight portion, Radix Et Caulis Acanthopanacis Senticosi 35 weight portion, white Rhizoma Curculiginis 15 weight portion, Herba Oxalidis strictae 15 weight portion, Herba Galii Bungei 20 weight portion, Radix et Rhizoma Thalictri 15 weight portion, Herba Taxilli 30 weight portion, Radix et Rhizoma Rhei Franzenbachii 20 weight portion, Radix Adenophorae 25 weight portion and Fructus Alpiniae Oxyphyllae 15 weight portion.
5. the preparation method of Traditional Chinese medicine immune preparation described in Claims 1-4, is characterized in that:
Each crude drug composition is crushed to 20 ~ 40 orders respectively, mixes by above-mentioned weight portion, then put it into volume and be 6 ~ 12 times of medical material amount and volumetric concentration is in the alcoholic solution of 70% ~ 95%, adopt reflux extraction, extract 2 ~ 4 times, each extraction time is 1.5 ~ 2.5h, and Extracting temperature is 65 DEG C ~ 85 DEG C, solution mixing, filtering and collecting filter liquid will be extracted, be placed on Rotary Evaporators concentrated, removing alcohol solvent, dry, pulverize, adaptive immune preparation.
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