CN104258047A - Traditional Chinese medicine composition for preventing and treating porcine reproductive and respiratory syndrome - Google Patents

Traditional Chinese medicine composition for preventing and treating porcine reproductive and respiratory syndrome Download PDF

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CN104258047A
CN104258047A CN201410534526.8A CN201410534526A CN104258047A CN 104258047 A CN104258047 A CN 104258047A CN 201410534526 A CN201410534526 A CN 201410534526A CN 104258047 A CN104258047 A CN 104258047A
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Qingdao Huaren Technology Incubator Co Ltd
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    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/35—Caprifoliaceae (Honeysuckle family)
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Abstract

The invention provides a traditional Chinese medicine composition for preventing and treating the porcine reproductive and respiratory syndrome. The composition comprises bulk drugs, namely, honeysuckle flower, cape jasmine fruit, common anemarrhena rhizome, commeline communis, amur cork-tree, danshen root, tuber fleeceflower stem, milkvetch root, sweet wormwood herb, myrrh, giant knotweed rhizome, chicken's gizzard-skin, yerbadetajo herb yerbadetajo herb, radix liriopes, barbated skullcup herb, root of Japanese campanumoea, sampson St.John's wort herb, Chinese taxillus herb, cassia twig, divaricate saposhnikovia root, small centipeda herb, Chinese yam, morinda root, garden euphorbia herb, hygrophila salicifolia nees, figwortflower picrorhiza rhizome and liquorice root. Compared with anti-virus Western medicines, the traditional Chinese medicine composition has the advantages that the composition is low in toxicity to animals, few in medicine residue and obvious in preventing and treating effect.

Description

For preventing and treating the Chinese medicine composition of Porcine reproductive and respiratory syndrome
Technical field
The present invention relates to field of Chinese herbal medicine technology, particularly relating to a kind of Chinese medicine composition for preventing and treating Porcine reproductive and respiratory syndrome.
Background technology
Porcine reproductive and respiratory syndrome (Porcine Reproductive and Respiration Syndrome, PRRS) be a kind of Acute exposure sexually transmitted disease caused by porcine reproductive and respiratory syndrome virus, mainly cause the breeding difficulty of in-pig and the respiratory symptom of piglet.Clinical symptoms main manifestations is sow heating, anorexia, miscarriage or stillborn fetus, mummy tire, and Respiratory symptoms and high mortality often appear in piglet.Since this disease was found first from 1987, all over the world each is raised pigs state now, and the pathological changes that it causes causes catastrophic strike to pig industry, and this sick viral pathogenesis power is strong, and spread speed is fast.Swinery M & M is high, and piglet sickness rate can reach 100%, mortality rate can reach more than 50%, and Sow abortion rate can reach more than 30%, and growing and fattening pigs, sow also can be fallen ill death.
The infection main damage macrophage of this disease, reduce immunity of organisms, cause secondary infection, difficulty of prevention and cure is large. and use antibiotic and antiviral agents easily to produce pollution and toxic and side effects. mainly by vaccination, this disease is prevented at present, there is no effective medicine.Attenuated vaccine immunity effect is better, but it is dangerous itself to there is loose poison, and after inactivated vaccine inoculation, the titre that specific antibody produces is low, and speed is slow, can only provide part immunoprotection.
The protection antibody horizontal and the cellular immunization that improve vaccine immunity are the keys that anti-porcine reproductive and respiratory syndrome virus infects.Chinese medicine aboundresources, in treatment disease of viral infection, have unique advantage, more Chinese medicine has immunoregulation effect and antivirus action, as health care anti-disease medicines and additive, the immunologic function of body can be improved, improve premunition and the resistance of body.The single medicinal material of viral infection resisting has virus replication or deactivation, stops the effect of virus absorption onto cell.
Theory of Chinese medical science is thought, though the generation of Porcine reproductive and respiratory syndrome and change intricate, be attributed to 2 points, i.e. body intrinsic factor and the interactional result of extrinsic factor.Traditional Chinese veterinary medicine thinks that this is the result that " healthy energy " struggles against with " pathogen "." healthy energy " is the functional activity of each internal organs organ of poultry body, and adaptive capacity, the resistivity to paathogenic factor, the rehabilitation ability of its environment to external world, be the identification foreign body based on the material such as function and qi-blood-body fluid, get rid of foreign body, maintain self homeostasis and stable function; " pathogen " refers to extraneous six climate exopathogens, the gas of epidemic disease, and whether the health comprising again pathological change that body imbalance of YIN and YANG produces and pathological product body depends on the power of " healthy energy " and " pathogen ".From traditional Chinese veterinary medicine angle analysis, Porcine reproductive and respiratory syndrome belongs to Epidemic Febrile Disease, and " exogenous pathogen mutual aid " is predisposing factors, and when the five internal organs that namely traditional Chinese medical science is said are in " void ", virus is taken advantage of a weak point, and causes pathogen being excessive but vital QI weak, disease-resistant unable; This disease is multiple for summer, how because of the damp and hot fire-transformation of diseases caused by exogenous pathogenic factor, causes caused by inhibited pulmonary QI, abnormal ascending-descending of QI, imbalance of YIN and YANG.In control, with strengthening vital QI to eliminate pathogenic factors, giving consideration to both the incidental and fundamental, by antiviral and immunity moderation function, realizes the preventing and controlling to this disease.Chinese medicine derives from natural, and poison the features such as little, drug residue is few to animal, compare antiviral Western medicine, Chinese medicine has more obvious advantage in anti-reproductive and respiratory syndrome processed.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of Chinese medicine composition feed additive for preventing and treating Porcine reproductive and respiratory syndrome and the feedstuff containing this Chinese medicine composition feed additive, compare antiviral Western medicine, it is little to animal murder by poisoning, drug residue is few, prevention and therapy successful, has advantage.
For solving the problems of the technologies described above, the present invention also provides a kind of feedstuff for preventing and treating Porcine reproductive and respiratory syndrome, and its raw material comprises Semen Maydis, bean cake, Sal, fish flour, Oleum Glycines, zinc methionine, copper lysine, tryptophan, VitAVitE, ethoxy quinoline and the Chinese medicine composition feed additive for preventing and treating Porcine reproductive and respiratory syndrome.
Wherein, in described feedstuff, the weight portion of each raw material is respectively Semen Maydis 280 weight portion ~ 320 weight portion, bean cake 140 weight portion ~ 160 weight portion, Sal 3 weight portion ~ 5 weight portion, fish flour 30 weight portion ~ 40 weight portion, Oleum Glycines 20 weight portion ~ 30 weight portion, zinc methionine 0.5 weight portion ~ 1.5 weight portion, copper lysine 0.5 weight portion ~ 1.5 weight portion, tryptophan 0.5 weight portion ~ 1.5 weight portion, retinol1 weight portion ~ 2 weight portion, vitamin e1 weight portion ~ 2 weight portion, ethoxy quinoline 2 weight portion ~ 4 weight portion, described Chinese medicine composition feed additive 50 weight portion ~ 70 weight portion.
Wherein, in described Chinese medicine composition feed additive, each crude drug comprises Flos Lonicerae, Fructus Gardeniae, the Rhizoma Anemarrhenae, Commeline communis, Cortex Phellodendri, Radix Salviae Miltiorrhizae, Caulis Polygoni Multiflori, the Radix Astragali, Herba Artemisiae Annuae, Myrrha, Rhizoma Polygoni Cuspidati, Endothelium Corneum Gigeriae Galli, Herba Ecliptae, Radix Liriopes, Herba Scutellariae Barbatae, Radix Campanumoeae, Herba Hyperici Sampsonii, Herba Taxilli, Ramulus Cinnamomi, Radix Saposhnikoviae, Herba Centipedae, Rhizoma Dioscoreae, Radix Morindae Officinalis, Ramulus et Folium Bauhiniae Faberi, Hygrophila salicifolia, Rhizoma Picrorhizae and Radix Glycyrrhizae.
Wherein, in described Chinese medicine composition feed additive, the weight of each crude drug is respectively Flos Lonicerae 55 weight portion ~ 65 weight portion, Fructus Gardeniae 30 weight portion ~ 40 weight portion, the Rhizoma Anemarrhenae 25 weight portion ~ 35 weight portion, Commeline communis 20 weight portion ~ 30 weight portion, Cortex Phellodendri 30 weight portion ~ 40 weight portion, Radix Salviae Miltiorrhizae 25 weight portion ~ 35 weight portion, Caulis Polygoni Multiflori 30 weight portion ~ 40 weight portion, the Radix Astragali 55 weight portion ~ 65 weight portion, Herba Artemisiae Annuae 25 weight portion ~ 35 weight portion, Myrrha 15 weight portion ~ 25 weight portion, Rhizoma Polygoni Cuspidati 20 weight portion ~ 30 weight portion, Endothelium Corneum Gigeriae Galli 30 weight portion ~ 40 weight portion, Herba Ecliptae 25 weight portion ~ 35 weight portion, Radix Liriopes 20 weight portion ~ 30 weight portion, Herba Scutellariae Barbatae 25 weight portion ~ 35 weight portion, Radix Campanumoeae 20 weight portion ~ 30 weight portion, Herba Hyperici Sampsonii 25 weight portion ~ 35 weight portion, Herba Taxilli 30 weight portion ~ 40 weight portion, Ramulus Cinnamomi 20 weight portion ~ 30 weight portion, Radix Saposhnikoviae 25 weight portion ~ 35 weight portion, Herba Centipedae 25 weight portion ~ 35 weight portion, Rhizoma Dioscoreae 20 weight portion ~ 30 weight portion, Radix Morindae Officinalis 20 weight portion ~ 30 weight portion, Ramulus et Folium Bauhiniae Faberi 25 weight portion ~ 35 weight portion, Hygrophila salicifolia 20 weight portion ~ 30 weight portion, Rhizoma Picrorhizae 15 weight portion ~ 25 weight portion and Radix Glycyrrhizae 20 weight portion ~ 30 weight portion.
Wherein, in described Chinese medicine composition feed additive, the weight of each crude drug is respectively Flos Lonicerae 60 weight portion, Fructus Gardeniae 35 weight portion, the Rhizoma Anemarrhenae 30 weight portion, Commeline communis 25 weight portion, Cortex Phellodendri 35 weight portion, Radix Salviae Miltiorrhizae 30 weight portion, Caulis Polygoni Multiflori 35 weight portion, the Radix Astragali 60 weight portion, Herba Artemisiae Annuae 30 weight portion, Myrrha 20 weight portion, Rhizoma Polygoni Cuspidati 25 weight portion, Endothelium Corneum Gigeriae Galli 35 weight portion, Herba Ecliptae 30 weight portion, Radix Liriopes 25 weight portion, Herba Scutellariae Barbatae 30 weight portion, Radix Campanumoeae 25 weight portion, Herba Hyperici Sampsonii 30 weight portion, Herba Taxilli 35 weight portion, Ramulus Cinnamomi 25 weight portion, Radix Saposhnikoviae 30 weight portion, Herba Centipedae 30 weight portion, Rhizoma Dioscoreae 25 weight portion, Radix Morindae Officinalis 25 weight portion, Ramulus et Folium Bauhiniae Faberi 30 weight portion, Hygrophila salicifolia 25 weight portion, Rhizoma Picrorhizae 20 weight portion and Radix Glycyrrhizae 25 weight portion.
Present invention also offers the preparation method of above-mentioned feedstuff, be specially:
The first step, mixes by weight by each raw material components, gets 100g mixture, add the water of 500g ~ 600g, boil 3 ~ 4 hours, filter, obtain the first filtrate, filtering residue adds the water of 200g ~ 300g again, again boils 1 ~ 2 hour, filter, obtain the second filtrate, by the first filtrate and the mixing of the second filtrate, concentrated removing aqueous solvent, pulverize, dry, obtain Chinese medicine composition fodder additive;
Second step, puts into fodder mixing machine together with the described Chinese medicine composition fodder additive other raw material components in feedstuff and the first step obtained, and continuous uniform stirred after 1 ~ 3 hour, put into pulverizer and pulverized, and obtains feedstuff.
Present invention also offers a kind of Chinese medicine composition for preventing and treating Porcine reproductive and respiratory syndrome, its crude drug comprises Flos Lonicerae, Fructus Gardeniae, the Rhizoma Anemarrhenae, Commeline communis, Cortex Phellodendri, Radix Salviae Miltiorrhizae, Caulis Polygoni Multiflori, the Radix Astragali, Herba Artemisiae Annuae, Myrrha, Rhizoma Polygoni Cuspidati, Endothelium Corneum Gigeriae Galli, Herba Ecliptae, Radix Liriopes, Herba Scutellariae Barbatae, Radix Campanumoeae, Herba Hyperici Sampsonii, Herba Taxilli, Ramulus Cinnamomi, Radix Saposhnikoviae, Herba Centipedae, Rhizoma Dioscoreae, Radix Morindae Officinalis, Ramulus et Folium Bauhiniae Faberi, Hygrophila salicifolia, Rhizoma Picrorhizae and Radix Glycyrrhizae.
Wherein, in described Chinese medicine composition, the weight of each crude drug is respectively Flos Lonicerae 55 weight portion ~ 65 weight portion, Fructus Gardeniae 30 weight portion ~ 40 weight portion, the Rhizoma Anemarrhenae 25 weight portion ~ 35 weight portion, Commeline communis 20 weight portion ~ 30 weight portion, Cortex Phellodendri 30 weight portion ~ 40 weight portion, Radix Salviae Miltiorrhizae 25 weight portion ~ 35 weight portion, Caulis Polygoni Multiflori 30 weight portion ~ 40 weight portion, the Radix Astragali 55 weight portion ~ 65 weight portion, Herba Artemisiae Annuae 25 weight portion ~ 35 weight portion, Myrrha 15 weight portion ~ 25 weight portion, Rhizoma Polygoni Cuspidati 20 weight portion ~ 30 weight portion, Endothelium Corneum Gigeriae Galli 30 weight portion ~ 40 weight portion, Herba Ecliptae 25 weight portion ~ 35 weight portion, Radix Liriopes 20 weight portion ~ 30 weight portion, Herba Scutellariae Barbatae 25 weight portion ~ 35 weight portion, Radix Campanumoeae 20 weight portion ~ 30 weight portion, Herba Hyperici Sampsonii 25 weight portion ~ 35 weight portion, Herba Taxilli 30 weight portion ~ 40 weight portion, Ramulus Cinnamomi 20 weight portion ~ 30 weight portion, Radix Saposhnikoviae 25 weight portion ~ 35 weight portion, Herba Centipedae 25 weight portion ~ 35 weight portion, Rhizoma Dioscoreae 20 weight portion ~ 30 weight portion, Radix Morindae Officinalis 20 weight portion ~ 30 weight portion, Ramulus et Folium Bauhiniae Faberi 25 weight portion ~ 35 weight portion, Hygrophila salicifolia 20 weight portion ~ 30 weight portion, Rhizoma Picrorhizae 15 weight portion ~ 25 weight portion and Radix Glycyrrhizae 20 weight portion ~ 30 weight portion.
Present invention also offers the preparation method of above-mentioned Chinese medicine composition, be specially: each raw material components is mixed by weight, get 100g mixture, add the water of 500g ~ 600g, boil 3 ~ 4 hours, filter, obtain the first filtrate, filtering residue adds the water of 200g ~ 300g again, again boils 1 ~ 2 hour, filter, obtain the second filtrate, by the first filtrate and the mixing of the second filtrate, concentrated removing aqueous solvent, pulverize, dry, obtain Chinese medicine composition.
Beneficial effect of the present invention:
Chinese medicine composition feed additive for preventing and treating Porcine reproductive and respiratory syndrome provided by the invention and the feedstuff containing this Chinese medicine composition feed additive, compare antiviral Western medicine, it is little to animal murder by poisoning, drug residue is few, and prevention and therapy successful, has advantage.
Detailed description of the invention
The invention provides a kind of Chinese medicine composition for preventing and treating Porcine reproductive and respiratory syndrome, its crude drug comprises Flos Lonicerae, Fructus Gardeniae, the Rhizoma Anemarrhenae, Commeline communis, Cortex Phellodendri, Radix Salviae Miltiorrhizae, Caulis Polygoni Multiflori, the Radix Astragali, Herba Artemisiae Annuae, Myrrha, Rhizoma Polygoni Cuspidati, Endothelium Corneum Gigeriae Galli, Herba Ecliptae, Radix Liriopes, Herba Scutellariae Barbatae, Radix Campanumoeae, Herba Hyperici Sampsonii, Herba Taxilli, Ramulus Cinnamomi, Radix Saposhnikoviae, Herba Centipedae, Rhizoma Dioscoreae, Radix Morindae Officinalis, Ramulus et Folium Bauhiniae Faberi, Hygrophila salicifolia, Rhizoma Picrorhizae and Radix Glycyrrhizae.
Further preferably, described Chinese medicine composition feed additive is only made up of above-mentioned raw materials medicine.
In described Chinese medicine composition, the weight of each crude drug is respectively Flos Lonicerae 55 weight portion ~ 65 weight portion, Fructus Gardeniae 30 weight portion ~ 40 weight portion, the Rhizoma Anemarrhenae 25 weight portion ~ 35 weight portion, Commeline communis 20 weight portion ~ 30 weight portion, Cortex Phellodendri 30 weight portion ~ 40 weight portion, Radix Salviae Miltiorrhizae 25 weight portion ~ 35 weight portion, Caulis Polygoni Multiflori 30 weight portion ~ 40 weight portion, the Radix Astragali 55 weight portion ~ 65 weight portion, Herba Artemisiae Annuae 25 weight portion ~ 35 weight portion, Myrrha 15 weight portion ~ 25 weight portion, Rhizoma Polygoni Cuspidati 20 weight portion ~ 30 weight portion, Endothelium Corneum Gigeriae Galli 30 weight portion ~ 40 weight portion, Herba Ecliptae 25 weight portion ~ 35 weight portion, Radix Liriopes 20 weight portion ~ 30 weight portion, Herba Scutellariae Barbatae 25 weight portion ~ 35 weight portion, Radix Campanumoeae 20 weight portion ~ 30 weight portion, Herba Hyperici Sampsonii 25 weight portion ~ 35 weight portion, Herba Taxilli 30 weight portion ~ 40 weight portion, Ramulus Cinnamomi 20 weight portion ~ 30 weight portion, Radix Saposhnikoviae 25 weight portion ~ 35 weight portion, Herba Centipedae 25 weight portion ~ 35 weight portion, Rhizoma Dioscoreae 20 weight portion ~ 30 weight portion, Radix Morindae Officinalis 20 weight portion ~ 30 weight portion, Ramulus et Folium Bauhiniae Faberi 25 weight portion ~ 35 weight portion, Hygrophila salicifolia 20 weight portion ~ 30 weight portion, Rhizoma Picrorhizae 15 weight portion ~ 25 weight portion and Radix Glycyrrhizae 20 weight portion ~ 30 weight portion.
Further, in described Chinese medicine composition, the weight of each crude drug is respectively Flos Lonicerae 58 weight portion ~ 62 weight portion, Fructus Gardeniae 33 weight portion ~ 37 weight portion, the Rhizoma Anemarrhenae 28 weight portion ~ 32 weight portion, Commeline communis 23 weight portion ~ 27 weight portion, Cortex Phellodendri 33 weight portion ~ 37 weight portion, Radix Salviae Miltiorrhizae 28 weight portion ~ 32 weight portion, Caulis Polygoni Multiflori 33 weight portion ~ 37 weight portion, the Radix Astragali 58 weight portion ~ 62 weight portion, Herba Artemisiae Annuae 28 weight portion ~ 32 weight portion, Myrrha 18 weight portion ~ 22 weight portion, Rhizoma Polygoni Cuspidati 23 weight portion ~ 27 weight portion, Endothelium Corneum Gigeriae Galli 33 weight portion ~ 37 weight portion, Herba Ecliptae 28 weight portion ~ 32 weight portion, Radix Liriopes 23 weight portion ~ 27 weight portion, Herba Scutellariae Barbatae 28 weight portion ~ 32 weight portion, Radix Campanumoeae 23 weight portion ~ 27 weight portion, Herba Hyperici Sampsonii 28 weight portion ~ 32 weight portion, Herba Taxilli 33 weight portion ~ 37 weight portion, Ramulus Cinnamomi 23 weight portion ~ 27 weight portion, Radix Saposhnikoviae 28 weight portion ~ 32 weight portion, Herba Centipedae 28 weight portion ~ 32 weight portion, Rhizoma Dioscoreae 23 weight portion ~ 27 weight portion, Radix Morindae Officinalis 23 weight portion ~ 27 weight portion, Ramulus et Folium Bauhiniae Faberi 28 weight portion ~ 32 weight portion, Hygrophila salicifolia 23 weight portion ~ 27 weight portion, Rhizoma Picrorhizae 18 weight portion ~ 22 weight portion and Radix Glycyrrhizae 23 weight portion ~ 27 weight portion.
Most preferably, in described Chinese medicine composition, the weight of each crude drug is respectively Flos Lonicerae 60 weight portion, Fructus Gardeniae 35 weight portion, the Rhizoma Anemarrhenae 30 weight portion, Commeline communis 25 weight portion, Cortex Phellodendri 35 weight portion, Radix Salviae Miltiorrhizae 30 weight portion, Caulis Polygoni Multiflori 35 weight portion, the Radix Astragali 60 weight portion, Herba Artemisiae Annuae 30 weight portion, Myrrha 20 weight portion, Rhizoma Polygoni Cuspidati 25 weight portion, Endothelium Corneum Gigeriae Galli 35 weight portion, Herba Ecliptae 30 weight portion, Radix Liriopes 25 weight portion, Herba Scutellariae Barbatae 30 weight portion, Radix Campanumoeae 25 weight portion, Herba Hyperici Sampsonii 30 weight portion, Herba Taxilli 35 weight portion, Ramulus Cinnamomi 25 weight portion, Radix Saposhnikoviae 30 weight portion, Herba Centipedae 30 weight portion, Rhizoma Dioscoreae 25 weight portion, Radix Morindae Officinalis 25 weight portion, Ramulus et Folium Bauhiniae Faberi 30 weight portion, Hygrophila salicifolia 25 weight portion, Rhizoma Picrorhizae 20 weight portion and Radix Glycyrrhizae 25 weight portion.
Present invention also offers the preparation method of above-mentioned Chinese medicine composition, be specially:
Each raw material components is mixed by weight, gets 100g mixture, add the water of 500g ~ 600g, boil 3 ~ 4 hours, filter, obtain the first filtrate, filtering residue adds the water of 200g ~ 300g again, again boils 1 ~ 2 hour, filters, obtain the second filtrate, by the first filtrate and the mixing of the second filtrate, concentrated removing aqueous solvent, pulverizes, drying, obtains Powdered additive.
This Chinese medicine composition uses as feed additive.
Present invention also offers the injection dosage form of above-mentioned Chinese medicine composition, its crude drug comprises Flos Lonicerae, Fructus Gardeniae, the Rhizoma Anemarrhenae, Commeline communis, Cortex Phellodendri, Radix Salviae Miltiorrhizae, Caulis Polygoni Multiflori, the Radix Astragali, Herba Artemisiae Annuae, Myrrha, Rhizoma Polygoni Cuspidati, Endothelium Corneum Gigeriae Galli, Herba Ecliptae, Radix Liriopes, Herba Scutellariae Barbatae, Radix Campanumoeae, Herba Hyperici Sampsonii, Herba Taxilli, Ramulus Cinnamomi, Radix Saposhnikoviae, Herba Centipedae, Rhizoma Dioscoreae, Radix Morindae Officinalis, Ramulus et Folium Bauhiniae Faberi, Hygrophila salicifolia, Rhizoma Picrorhizae and Radix Glycyrrhizae, its preparation method is specially: mixed by weight by each raw material components, get 100g mixture, add the water of 500g ~ 600g, boil 3 ~ 4 hours, filter, obtain the first filtrate, filtering residue adds the water of 200g ~ 300g again, again boil 1 ~ 2 hour, filter, obtain the second filtrate, by the first filtrate and the mixing of the second filtrate, concentrated removing aqueous solvent, pulverize, dry, obtain Chinese medicine composition, described Chinese medicine composition is dissolved in water, adjust pH 7.0, filter, embedding, sterilizing, obtaining crude drug concentration is the injection of 1g/ml.
The present invention also provides a kind of feedstuff for preventing and treating Porcine reproductive and respiratory syndrome, and its raw material comprises Semen Maydis, bean cake, Sal, fish flour, Oleum Glycines, zinc methionine, copper lysine, tryptophan, VitAVitE, ethoxy quinoline and the Chinese medicine composition feed additive for preventing and treating Porcine reproductive and respiratory syndrome.
Further, in described feedstuff, the weight portion of each raw material is respectively Semen Maydis 280 weight portion ~ 320 weight portion, bean cake 140 weight portion ~ 160 weight portion, Sal 3 weight portion ~ 5 weight portion, fish flour 30 weight portion ~ 40 weight portion, Oleum Glycines 20 weight portion ~ 30 weight portion, zinc methionine 0.5 weight portion ~ 1.5 weight portion, copper lysine 0.5 weight portion ~ 1.5 weight portion, tryptophan 0.5 weight portion ~ 1.5 weight portion, retinol1 weight portion ~ 2 weight portion, vitamin e1 weight portion ~ 2 weight portion, ethoxy quinoline 2 weight portion ~ 4 weight portion, Chinese medicine composition feed additive 50 weight portion ~ 70 weight portion.
Further, in described feedstuff, the weight portion of each raw material is respectively Semen Maydis 300 weight portion, bean cake 150 weight portion, Sal 4 weight portion, fish flour 35 weight portion, Oleum Glycines 25 weight portion, zinc methionine 1 weight portion, copper lysine 1 weight portion, tryptophan 1 weight portion, vitamin A 2 weight portion, vitamin E 2 weight portion, ethoxy quinoline 3 weight portion, Chinese medicine composition feed additive 60 weight portion.
Present invention also offers the preparation method of above-mentioned feedstuff, be specially:
The first step, prepares described Chinese medicine composition feed additive;
Second step, puts into fodder mixing machine together with the described Chinese medicine composition feed additive other raw material components in feedstuff and the first step obtained, and continuous uniform stirred after 1 ~ 3 hour, put into pulverizer and pulverized, and obtains feedstuff.
The pharmacology of described each raw material of Chinese medicine medicine is as follows:
Flos Lonicerae: latin name lonicerae japonicae flos, sweet in the mouth, cold in nature, enter the heart, lung, stomach, large intestine channel, have the effect of heat-clearing and toxic substances removing, holding concurrently can eliminating pathogenic heat from blood to cure dysentery.Be mainly used in affection due to external wind and heat, epidemic febrile disease pyretic toxicity is comparatively contained, or when epidemic febrile disease is still not descending at the part of the body cavity above the diaphragm housing the heart and lungs from the beginning of pathogenic heat; Pyretic toxicity dysentery, stool hematochezia; Fever disease in summer is demonstrate,proved; The carbuncle sore that pyretic toxicity gathers is from the beginning of the, inflammation heat person of swelling and ache etc., there is good antipyretic effect, energy antiinflammatory, what control inflammation oozes out and inflammatory hyperplasia, leukocytic phagocytic function can be promoted, there is stronger bacteriostasis to multiple pyococcus and pathogenic entero becteria, comprise staphylococcus, Hemolytic streptococcus, meningococcus, dysentery bacterium, Bacillus typhi, bacillus pyocyaneus, tubercule bacillus etc.
Fructus Gardeniae: latin name gardeniae fructus, bitter in the mouth, cold in nature, enter the heart, lung, three burnt, have the effect of relieving restlessness pathogenic fire purging, dispersing the stagnated live-QI to relieve the stagnation of QI, clearing away heat-damp and promoting diuresis, cooling blood for hemostasis, be mainly used in the vexed uneasiness of epidemic febrile disease heat stagnation breast gastral cavity, bother not peaceful; Stagnation of liver-QI is not relaxed or Liver Channel has having a dizzy spell of heat, pain in chest and hypochondrium, conjunctival congestion dry pharynx; Jaundice due to damp-heat; The cards such as the abdominal distention of qi depression to blood stasis.
The Rhizoma Anemarrhenae: bitter in the mouth, sweet, cold in nature, enters lung, stomach, kidney channel, has relieving restlessness of purging heat, the effect of nourshing Yin and drynsessmoistening prescription, clearing away lung-heat to relieve cough, and cure mainly fever caused by exogenous pathogenic factors, high heat is irritated, lung-heat type cough, and interior-heat such as to be quenched one's thirst at the symptom; Its bitter cold matter is moistened, and property sedimentation can heat clearing away, can promote the production of body fluid again, kindly controls warm pathogenic factor and enters edema caused by disorder of QI, intenseness of heat impairment of body fluid; Also diabetes is used for the treatment of; Phlegm-heat obstructing in the lung and breathing heavily, xeropulmonary cough, expectorant is few unfavorable; Hyperactivity of fire caused by deficiency of YIN, the cards such as hyperactivity of deficient fire.
Commeline communis: sweet in the mouth, light, cold in nature, enter the heart, liver,spleen,kidney, intestine and small intestine warp, have heat-clearing and toxic substances removing, effect of inducing diuresis to remove edema.Cure mainly edema, beriberi, dysuria, flu, erysipelas, parotitis, icterohepatitis, hematodiarrhoea, malaria, epistaxis, hematuria, metrorrhagia, leucorrhea, laryngopharynx swelling and pain, the cards such as carbuncle furuncle.
Cortex Phellodendri: bitter in the mouth, cold in nature, enter kidney, bladder, large intestine channel, heat clearing and damp drying, eliminating fire and detoxication, except hectic fever due to YIN-deficiency clearind deficient heat.For damp-heat dysentery, jaundice, leukorrhagia, pyretic stranguria, beriberi, flaccidity is warded off, hectic fever due to YIN-deficiency consumptive fever, night sweat, seminal emission, sore swollen toxin, eczema pruritus.Stir-baked CORTEX PHELLODENDRI with salt solution nourishing YIN to lower pathogenic fire.For hyperactivity of fire caused by deficiency of YIN, night sweat hectic fever due to YIN-deficiency.
Radix Salviae Miltiorrhizae: bitter in the mouth, cold nature, enters the heart, Liver Channel, has promoting blood flow to regulate menstruation, stasis-dispelling and pain-killing, removing heat from blood eliminating carbuncle, clear away heart-fire relieving restlessness, the effect of nourishing blood to tranquillize the mind, for menoxenia, dysmenorrhea, amenorrhea, the stagnant stomachache of the stasis of blood in puerperal, trusted subordinate's pain, pyretic arthralgia swells and ache, traumatic injury, and calentura is irritated, the cards such as palpitation and insomnia, Radix Salviae Miltiorrhizae energy coronary artery dilator, increases coronary flow, improves myocardial ischemia, infraction and cardiac function, regulate the rhythm of the heart, and can peripheral blood vessel be expanded, improve microcirculation; Body's hypoxia tolerance can be improved; There is anticoagulation, promote fibrinolytic, suppress platelet aggregation, the effect of inhibition thrombosis; Can blood fat be reduced, suppress coronary atherosclerosis to be formed; Can suppress or alleviate hepatocellular degeneration, necrosis and inflammatory reaction, promote liver cell regeneration, and have anti-fibrosis effect; The convalescent period of erythrocyte and hematochrome can be shortened, Reticulocyte is increased, the reparation organized can be promoted, accelerate the healing of fracture; Nervus centralis is had to have inhibitory action; There is antitumor action; Energy enhancing human body immunity function; Blood glucose can be reduced; Inhibitory action is had to the various bacteria such as tubercule bacillus, Staphyloccocus aureus.
Caulis Polygoni Multiflori: sweet in the mouth, micro-hardship, property is put down; GUIXIN, Liver Channel, have effect of energy tranquilizing by nourishing the heart, dispelling wind and removing obstruction in the collateral, cure mainly insomnia and dreamful sleep, blood deficiency general pain, numbness of the skin, rheumatic arthralgia, the cards such as wind skin ulcer scabies.
The Radix Astragali: latin name astragali radix, sweet in the mouth, warm in nature, return lung, spleen channel, have tonification spleen soil, elevate a turnable ladder yang-energy, strengthening superficial resistance to stop perspiration, the effects such as holder skin ulcer granulation promoting, are mainly used in treating the caused weak anorexia and loose stool of deficiency of both the splenic and pulmonary QI, shortness of breath and palpitation, the visceroptosis of sinking of QI of middle-JIAO, the metrorrhagia of QI failing to control blood is had blood in stool, chronic diarrhea proctoptosis; The spontaneous sweating of exterior deficiency; Insufficiency of vital energy and blood, yang-energy skin infection on the decline does not rise, difficulty in discharging of pus, burst and do not hold back for a long time; And the deficiency of vital energy loses the edema of fortune, the cards such as dysuria.
Herba Artemisiae Annuae: latin name Artemisiae Annuae Herba, bitter in the mouth, pungent, cold in nature, return liver, gallbladder meridian, have deficiency-heat clearly thoroughly, removing heat from blood is except steaming, and except steaming, effect of preventing the attack (or recurrence) of malaria, cures mainly pathogenic summer-heat heating, fever due to yin deficiency, night fever abating at dawn, hectic fever due to YIN-deficiency consumptive fever, malaria cold and heat, the cards such as jaundice due to damp-heat.
Myrrha: bitter in the mouth, pungent, property is put down, and enters liver, spleen, the heart, kidney channel, has effect of promoting blood circulation and stopping pain, detumescence and promoting granulation, cure mainly the cards such as breast abdomen stasis of blood pain, dysmenorrhea, amenorrhea, lump in the abdomen, traumatic injury, carbuncle skin infection, acute appendicitis, conjunctival congestion and swelling pain.
Rhizoma Polygoni Cuspidati: latin name Polygoni Cuspidati Rhizoma et Radix, micro-hardship, is slightly cold.Return liver, gallbladder, lung meridian, there is heat-clearing and toxic substances removing, promoting the function of the gallbladder to alleviate jaundice, expelling wind and removing dampness, dissipating blood stasis analgesic therapy, effect of relieving cough and resolving phlegm, for arthralgia, jaundice due to damp-heat, amenorrhea ， WEIJIA, cough with copious phlegm, burn due to hot liquid or fire, injury from falling down, the cards such as carbuncle sore tumefacting virus.
Endothelium Corneum Gigeriae Galli: sweet in the mouth, cold in nature, return spleen, stomach, small intestinal, urinary bladder channel, have strengthening the spleen to promote digestion, arresting seminal emission stops the effect of drowning, cure mainly food stagnation distension, vomiting regurgitation, dysentery, infantile malnutrition, quenches one's thirst, incontinence of urine, sore throat tonsillitis, the cards such as ulcerative gingivitis aphtha.
Herba Ecliptae: sweet in the mouth, acid, cold in nature, return liver, kidney channel, play not liver and kidney tonifying, the effect of cooling blood for hemostasis, for having a dizzy spell caused by the hepatic and renal YIN deficiency, blurring of vision, soreness of the waist and knees; Be suitable for the deficiency of YIN and have hot person, be used for the treatment of the haematemesis of accumulated heat in stomach, the cards such as the hemoptysis of injury of blood vessel of the lung by heat.
Radix Liriopes: latin name liriopes radix, sweet in the mouth, micro-hardship, cold nature, GUIXIN, lung, stomach warp, have YIN nourishing and the production of body fluid promoting, effect that lung moistening clears away heart-fire, and for dryness of the lung dry cough, cough due to consumptive disease, Tianjin wound is thirsty, vexed insomnia, the cards such as dryness of the intestine constipation.
Herba Scutellariae Barbatae: acrid in the mouth, hardship, cold in nature, enter lung meridian, Liver Channel, kidney channel, there is the effect of heat-clearing and toxic substances removing, dissipating blood stasis hemostasis, inducing diuresis to remove edema, cure mainly: pyretic toxicity carbuncle; Have sore throat; Lung abscess; Intestinal carbuncle; Scrofula; Venom; Traumatic injury; Spit blood; Epistaxis; Stranguria with blood; Edema; Ascites and cancer.
Radix Campanumoeae: latin name radix campanumoea javanicae is the root of campanulaceae leopard platymiscium Campanumoea javanica and leopard, sweet in the mouth, property is put down, and returns lung, spleen channel, has invigorating the spleen and benefiting QI, tonifying the lung and stopping cough, effect of stimulating milk secretion, cure mainly the empty internal injury of labor, the deficiency of vital energy is weak, cardiopalmus, hyperhidrosis, diarrhea due to hypofunction of the spleen, leucorrhea, milk is rare, the cards such as insufficiency of the spleen cough.
Herba Hyperici Sampsonii: the herb for Guttiferae hypericum Herba Hyperici Sampsonii (nontoxic): bitter in the mouth, pungent, cold in nature, return liver, spleen channel, there is removing pathogenic heat from blood and toxic substance from the body, promoting blood flow to regulate menstruation, effect of dispelling wind and removing obstruction in the collateral, cure mainly various hemorrhage, enteritis and dysentery, acute mastitis, carbuncle furunculosis, scalds, venom, menoxenia, dysmenorrhea, leucorrhea, traumatic injury, rheumatic arthralgia, the cards such as aphtha.
Herba Taxilli: bitter in the mouth, sweet, property is put down, and enters liver, kidney channel, have the effect of invigorating the liver and kidney, bone and muscle strengthening, wind-damp dispelling, antiabortive unit, for the soreness of waist pain caused by deficiency of the liver and kindey, muscles and bones flaccidity is soft, the foot and knee being feeble, deficiency of the liver and kindey, essence and blood void is lost, the frequent fetal movement of liability to abortion, the cards such as waist abdomen pain.
Ramulus Cinnamomi: be the dry twig of canella Cortex Cinnamomi: latin name Cinnamomi Ramulus, acrid in the mouth, sweet, warm in nature, GUIXIN, lung, urinary bladder channel, have diaphoresis expelling pathogenic factors from muscles, warming the meridian and promoting blood circulation, supporing yang activating QI, effect of dispersing cold for relieving pain.
Radix Saposhnikoviae: acrid in the mouth, sweet, slightly warm in nature, returns bladder, liver, spleen channel, has expelling pathogenic wind from the body surface, removing dampness to relieve pain, and effect that relieving convulsion is jerked surely cures mainly diseases caused by exogenous pathogenic factor exterior syndrome, rubella pruritus, rheumatic arthralgia, the cards such as tetanus.
Herba Centipedae: acrid in the mouth, warm in nature, enter lung, Liver Channel, there is cold-dispelling, clearing the nasal passage, eliminating phlegm and stopping cough, the effect of dissipating blood stasis for subsidence of swelling, for exterior syndrome due to wind-cold, nasal obstruction watery nasal discharge and nasal sinusitis, cough with copious phlegm, pertussis, the cards such as sore, carbuncle and painful swelling.
Rhizoma Dioscoreae: latin name dioscoreae rhizoma, sweet in the mouth, property are flat, and enter lung, spleen, stomach warp, have spleen reinforcing nourishing the stomach, lung benefiting reinforces the kidney, and the effect of YIN nourishing and the production of body fluid promoting, cures mainly weakness of the spleen and stomach, the lack of appetite poor appetite of dysfunction of the spleen in transportation, the cards such as the vexed diarrhoea of gastral cavity.
Radix Morindae Officinalis: acrid in the mouth, sweet, slightly warm in nature, enters liver, kidney channel, has the effect of kidney-replenishing, bone and muscle strengthening, wind-damp dispelling, and for impotence due to deficiency of the kidney, seminal emission premature ejaculation, few abdomen cold type of pain, cold womb is infertile, the cards such as the muscles and bones flaccidity caused by deficiency of the liver and kindey is soft, chills and pain of the waist and kness.
Ramulus et Folium Bauhiniae Faberi: mildly bitter flavor, micro-acid, cool in nature, there is heat-clearing and toxic substances removing, effect that dampness removing is antipruritic, for bacillary dysentery, amebic dysentery, enteritis, intestinal infusorian, dyspepsia, bronchitis, pyelonephritis; The cards such as eczema, dermatitis, skin pruritus are controlled in external.
Hygrophila salicifolia: sweet in the mouth, micro-hardship, cool in nature, have heat-clearing and toxic substances removing, effect of dissipating blood stasis for subsidence of swelling, row pyretic toxicity when curing mainly, erysipelas, jaundice, aphtha, laryngopharynx swelling and pain, acute mastitis, traumatic injury, fracture, snake venom such as to bite at the card.
Rhizoma Picrorhizae: latin name Picrorhizae Rhizoma, bitter in the mouth, cold in nature, return liver, stomach, large intestine channel, have reducing the asthenic fever, dispelling fever with infantile malnutrition, effect of clearing away damp-heat, cures mainly osteopyrexia and fever, children's's infantile malnutrition with fever, damp-heat dysentery, jaundice dark coloured urine, the cards such as swelling and pain of hemorrhoid.
Radix Glycyrrhizae: latin name Glycyrrhizae Radix et Rhizoma, sweet in the mouth, property put down, enter 12 warps, have heat-clearing and toxic substances removing, be in harmonious proportion the property of medicine, the effect of expelling phlegm for arresting cough, and can spleen invigorating and in, relieving spasm to stop pain, is mainly used in the abdominal distension and anorexia of weakness of the spleen and stomach, weak; Can alleviate contracture pain in abdomen, relax the strong and toxicity of some drugs, alleviating medicine stimulates to the toxic and side effects of body or to gastrointestinal, can play QI invigorating neutralization by adding Radix Glycyrrhizae, is in harmonious proportion the work of each component.
Below adopt embodiment to describe embodiments of the present invention in detail, to the present invention, how application technology means solve technical problem whereby, and the implementation procedure reaching technique effect can fully understand and implement according to this.
Embodiment 1 Chinese medicine composition feed additive 1
By Flos Lonicerae 60g, Fructus Gardeniae 35g, Rhizoma Anemarrhenae 30g, Commeline communis 25g, Cortex Phellodendri 35g, Radix Salviae Miltiorrhizae 30g, Caulis Polygoni Multiflori 35g, Radix Astragali 60g, Herba Artemisiae Annuae 30g, Myrrha 20g, Rhizoma Polygoni Cuspidati 25g, Endothelium Corneum Gigeriae Galli 35g, Herba Ecliptae 30g, Radix Liriopes 25g, Herba Scutellariae Barbatae 30g, Radix Campanumoeae 25g, Herba Hyperici Sampsonii 30g, Herba Taxilli 35g, Ramulus Cinnamomi 25g, Radix Saposhnikoviae 30g, Herba Centipedae 30g, Rhizoma Dioscoreae 25g, Radix Morindae Officinalis 25g, Ramulus et Folium Bauhiniae Faberi 30g, Hygrophila salicifolia 25g, Rhizoma Picrorhizae 20g and Radix Glycyrrhizae 25g mixes, get 100g mixture, add the water of 600g, boil 4 hours, filter, obtain the first filtrate, filtering residue adds the water of 300g again, again boil 2 hours, filter, obtain the second filtrate, by the first filtrate and the mixing of the second filtrate, concentrated removing aqueous solvent, pulverize, dry, obtain Powdered additive.
Embodiment 2 Chinese medicine composition feed additive 2
By Flos Lonicerae 55g, Fructus Gardeniae 30g, Rhizoma Anemarrhenae 25g, Commeline communis 20g, Cortex Phellodendri 30g, Radix Salviae Miltiorrhizae 25g, Caulis Polygoni Multiflori 30g, Radix Astragali 55g, Herba Artemisiae Annuae 25g, Myrrha 15g, Rhizoma Polygoni Cuspidati 20g, Endothelium Corneum Gigeriae Galli 30g, Herba Ecliptae 25g, Radix Liriopes 20g, Herba Scutellariae Barbatae 25g, Radix Campanumoeae 20g, Herba Hyperici Sampsonii 25g, Herba Taxilli 30g, Ramulus Cinnamomi 20g, Radix Saposhnikoviae 25g, Herba Centipedae 25g, Rhizoma Dioscoreae 20g, Radix Morindae Officinalis 20g, Ramulus et Folium Bauhiniae Faberi 25g, Hygrophila salicifolia 20g, Rhizoma Picrorhizae 15g and Radix Glycyrrhizae 20g mixes, get 100g mixture, add the water of 600g, boil 4 hours, filter, obtain the first filtrate, filtering residue adds the water of 300g again, again boil 2 hours, filter, obtain the second filtrate, by the first filtrate and the mixing of the second filtrate, concentrated removing aqueous solvent, pulverize, dry, obtain Powdered additive.
Embodiment 3 Chinese medicine composition feed additive 3
By Flos Lonicerae 65g, Fructus Gardeniae 40g, Rhizoma Anemarrhenae 35g, Commeline communis 30g, Cortex Phellodendri 40g, Radix Salviae Miltiorrhizae 35g, Caulis Polygoni Multiflori 40g, Radix Astragali 65g, Herba Artemisiae Annuae 35g, Myrrha 25g, Rhizoma Polygoni Cuspidati 30g, Endothelium Corneum Gigeriae Galli 40g, Herba Ecliptae 35g, Radix Liriopes 30g, Herba Scutellariae Barbatae 35g, Radix Campanumoeae 30g, Herba Hyperici Sampsonii 35g, Herba Taxilli 40g, Ramulus Cinnamomi 30g, Radix Saposhnikoviae 35g, Herba Centipedae 35g, Rhizoma Dioscoreae 30g, Radix Morindae Officinalis 30g, Ramulus et Folium Bauhiniae Faberi 35g, Hygrophila salicifolia 30g, Rhizoma Picrorhizae 25g and Radix Glycyrrhizae 30g mixes, get 100g mixture, add the water of 600g, boil 4 hours, filter, obtain the first filtrate, filtering residue adds the water of 300g again, again boil 2 hours, filter, obtain the second filtrate, by the first filtrate and the mixing of the second filtrate, concentrated removing aqueous solvent, pulverize, dry, obtain Powdered additive.
Embodiment 4 pharmacological toxicity is tested
Acute toxicity test data
Detectable
Serum AST, ALT detection kit are purchased from Rui Sheng bio tech ltd, Qingdao.
Checkout equipment
Micro-200 semiautomatic biochemistry detector, analytical balance, dissecting instrument, syringe, test tube etc.
Test method
After the adaptabilities of 3 days are raised, be divided at random 3 groups (male and female half and half), often organize each 20; Matched group, 2 different pharmaceutical groups (are respectively 400g/kg body weight, 300g/kg body weight), matched group gavage normal saline, the Chinese medicine composition feed additive 1 of 2 the medicine group gavage embodiment of the present invention 1 preparations, is dissolved in Chinese medicine composition feed additive in water and gavages mice.
Testing index and method
(1) clinicing symptom observation: every day viewing test Mus the mental status, diet situation, every day entry morbidity death condition.
(2) pathological observation: dead Mus is carried out cuing open inspection and observes internal organs change.
(3) growth indexes is observed: carry out routine weighing to test Mus; Off-test, slaughters that Mus weighs in, the heavy organ index of internal organs measures: cut open inspection rat, core dirty, spleen, liver, kidney, testis, ovary, weigh, calculate organ index according to the following formula: organ index=internal organs weight/body weight × 100.
(4) liver function change is observed: after off-test, slaughter Mus, take blood separation serum, measures AST, ALT.
Data process&analysis
Each group of data all use mean soil standard deviation represent, each group mean does test of normality and repeated measure homogeneity test of variance, with inspection younger between one factor analysis of variance group), P < 0.05 is for differing significantly, and P < 0.01 is difference highly significant.Statistical computation all adopts SPSS12.0 software to complete.
Clinicing symptom observation
Matched group and test group Mus, the mental status and diet wish, to external world respond have no obvious exception.Each group of Mus morbidity and death condition are as following table 1.
Table 1
Grouping Sum Dead (only) Mortality rate (%)
1(400g/kg) 20 0 0
2(300g/kg) 20 0 0
Matched group 20 0 0
Pathological observation: matched group and test group, Mus carries out cuing open inspection and observes to have no and observe the obvious pathological change of internal organs after slaughtering.
Growth indexes is observed: off-test, slaughters that Mus weighs in, internal organs weight analysis is in table 2.
Table 2
Grouping Liver index Cardiac index Renal index Lungs index Index and spleen index
1(400g/kg) 5.79±0.22 0.83±0.08 1.23±0.21 0.72±0.09 0.51±0.06
2(300g/kg) 5.73±0.17 0.80±0.11 1.21±0.19 0.74±0.12 0.52±0.08
Matched group 5.72±0.25 0.82±0.09 1.26±0.25 0.73±0.10 0.50±0.05
Liver function change is observed
After off-test, slaughter Mus, take blood separation serum, measure AST (glutamic oxaloacetic transaminase, GOT), ALT (glutamate pyruvate transaminase) in table 3.It is not remarkable that result shows each group of data difference compared with normal healthy controls group, is all in normal range.
Table 3
Grouping Sum ALT AST
1(400g/kg) 20 30.91±2.17 132.11±10.32
2(300g/kg) 20 32.63±2.53 130.57±9.96
Matched group 20 31.97±2.09 131.91±12.03
Long term toxicity test data
Chinese medicine composition feed additive 20g/kg, 100g/kg of the present invention of preparing according to embodiment 1 were to the continuous gastric infusion of rat 3 months, the fur of animal, the colour of skin, ingest, activity, the blood parameters such as routine blood test, Liver and kidney function of the animal all without exception such as urine, feces and animal main organs index all in normal range, and normal kinetic zero difference histopathologic examination shows, the important organs such as the heart, liver, spleen, lung, kidney, adrenal gland, trachea, testis, ovary are without pathological change.Drug withdrawal checks above indices all without overt toxicity reaction after two weeks, show without cumulative toxicity.
Embodiment 5 feedstuff
Chinese medicine composition feed additive 60kg Semen Maydis 300kg, bean cake 150kg, Sal 4kg, fish flour 35kg, Oleum Glycines 25kg, zinc methionine 1kg, copper lysine 1kg, tryptophan 1kg, vitamin A 2kg, vitamin E 2kg, ethoxy quinoline 3kg, embodiment 1 prepared puts into fodder mixing machine together, after continuous uniform stirs 3 hours, put into pulverizer to pulverize, obtain feedstuff.
Embodiment 6 Viral Assay
Strain, cell strain
PRRSV PX strain, Marc-145 cell are preserved .PRRSV PX strain by Guangdong Provincial Academy Of Agricultural Sciences Veterinary Institute infecting both domestic animals and human ward and are thawed in 37 DEG C before use, are diluted to 100TCID50 for subsequent use with Marc-145 cell maintenance medium.
Medicine, reagent and culture medium
Ribavirin injection is provided by Shanghai Ha Sen Pharma Inc., and content is 0.1g/ml.Culture medium is DMEM+10%FBS.
Chinese medicine composition feed additive embodiment 1 prepared is dissolved in water, adjust pH 7.0, filters, embedding, sterilizing, and obtaining crude drug concentration is the injection of 1g/ml.
To the mensuration of Marc-145 cell maximum safe concentration
Marc-145 cell is inoculated on 96 porocyte culture plates, cultivates 24h and form fine and close monolayer, abandon supernatant.Respectively Chinese medicine composition composition injection, 0.1g/ml ribavirin injection DMEM maintenance medium are first done 10 times of dilutions, then continuous 2 doubling dilutions are remake, make 12 diluted concentrations respectively, namely injection diluted concentration is: 112,56,28,14,7,3.5,1.75,0.875,0.4375,0.2188,0.1094,0.055mg/ml; Ribavirin injection diluted concentration is: 10,5,2.5,1.25,0.625,0.3125,0.156,0.078,0.039,0.0195,0.00975,0.0049mg/ml.Each dilution factor solution is added to length have on 96 porocyte culture plates of monolayer Marc-145 cell, each dilution factor repeats 4 holes, every hole 100 μ L.Separately replace medicinal liquid as normal cell controls using DMEM cell maintenance medium, put 37 DEG C, 5%CO 2continuous 3d observation of cell pathological changes situation after cultivating 24h in constant temperature cell culture incubator.Finally judge that medicine is to the toxicity of cell, not occur that cytopathic maximum drug level is for maximum safe concentration according to cytopathy.
PRRSV standard strain TCID 50mensuration
Marc-145 cell is inoculated on 96 porocyte culture plates, cultivates 24h and form fine and close cell monolayer, abandon supernatant.PRRSV standard strain is made continuous 10 doubling dilutions and become 6 concentration, be added on 96 porocyte culture plates, each dilution factor repeats 6 holes, every hole 100 μ L, and other are all set to cell controls group, put 37 DEG C, 5%CO 2cultivate in constant temperature cell culture incubator.By inverted microscope observation of cell pathological changes situation after 72h, the cytopathy (cPE) that record produces, till the most high dilution of virus no longer produces new pathological changes.According to Reed-Muench formula, calculate the half cell infection amount (TCID of PRRSV 50).
Chinese medicine composition feed additive is tested the interaction in vitro of PRRSV
Chinese medicine composition measures PRRSV inhibitory action on a cellular level
Marc-145 cell is inoculated on 96 porocyte culture plates, cultivates 24h and form fine and close cell monolayer, abandon supernatant.First Chinese medicine composition composition injection, ribavirin injection are made continuous 2 doubling dilutions from maximum safe concentration, add on 96 porocyte culture plates, every hole 100 μ l, is placed in 37 DEG C of 2h; Again will containing 100 × TCID 50virus liquid be added to above-mentioned having added in the cell hole of medicine, each dilution factor repeats 4 holes, every hole 100 μ l.All set up normal cell controls and virus control, put 37 DEG C, 5%CO 2cultivate in constant incubator.After 24h, continuous 4d observes pathological changes situation.
Chinese medicine composition composition injection measures PRRSV killing action on a cellular level
Marc-145 cell is inoculated on 96 porocyte culture plates, cultivates 24h and form fine and close cell monolayer, abandon supernatant.Chinese medicine composition composition injection, ribavirin solution are made 2 doubling dilutions from maximum safe concentration, adds equivalent 200 × TCID 50virus liquid, mixing is placed on 37 DEG C and hatches 2h, and add in 96 porocyte culture plates, each dilution factor repeats 4 holes, every hole 100 μ l.All set up normal cell controls and virus control, put 37 DEG C, 5%CO 2cultivate in constant incubator.After 24h, continuous 4d observes pathological changes situation.
Chinese medicine composition composition injection measures PRRSV blocking effect on a cellular level
Marc-145 cell is inoculated on 96 porocyte culture plates, cultivates 24h and form fine and close cell monolayer, abandon supernatant.First will containing 100 × TCID 50virus liquid be added in 96 porocyte culture plates, every hole 100 μ L, be placed in 37 DEG C effect 2h after, abandon supernatant.Again Radix Bupleuri removing toxic substances, ribavirin injection are made 2 doubling dilutions from maximum safe concentration, be added in the above-mentioned cell hole having infected virus, each dilution factor repeats 4 holes, every hole 100 μ L.All set up normal cell controls and virus control, put 37 DEG C, 5%CO 2cultivate in constant incubator.Continuous 4d observation of cell pathological changes situation after 24h.
Cytopathy criterion
The percent of cell generation pathological changes is represented with "+" number, 25% hereinafter referred to as CPE (+), 26% ~ 50% is expressed as CPE (++), and 51% ~ 75% is expressed as CPE (+++), and 76% ~ 100% is expressed as CPE (++++).Cell controls form is all normal, and be expressed as CPE (), virus control all produces pathological changes, is expressed as CPE (++++).
Results and analysis
Chinese medicine composition composition injection is to the measurement result of Marc-145 cell maximum safe concentration
As known from Table 4, Chinese medicine composition composition injection maximum safe concentration on Marc-145 cell is 6000 μ g/mL, and ribavirin injection maximum safe concentration is 39 μ g/mL.
Table 4
Solution Cell hole is without the maximum safety of medicine concentration (μ g/mL) during CPE
Chinese medicine composition composition injection 6000
Ribavirin injection 39
The TCID of PRRSV 50measurement result
According to Reed-Muench formula, calculate the TCID of PRRSV at Marc-14 cell 50for 105.5/0.1mL.
Result of the test, in Table 5-table 7.
The inhibition of table 5 couple PRRSV
Solution Cell hole is without the solution dilution degree (μ g/mL) during CPE
Chinese medicine composition composition injection 20
Ribavirin injection 0.14
As can be seen from Table 5, Chinese medicine composition feed additive has certain inhibitory action to PRRSV.
The killing effect of table 6 couple PRRSV
Solution Cell hole is without the solution dilution degree (μ g/mL) during CPE
Chinese medicine composition composition injection All without CPE
Ribavirin injection All without CPE
As can be seen from Table 6, Chinese medicine composition feed additive has good bactericidal action to PRRSV.
The blocking effect of table 7 couple PRRSV
Solution Cell hole is without the solution dilution degree (μ g/mL) during CPE
Chinese medicine composition composition injection 130
Ribavirin injection All without CPE
As shown in Table 7, Chinese medicine composition feed additive of the present invention has blocking effect to PRRSV.
Embodiment 7 effect test
Trial drug
Feedstuff prepared by embodiment 4.
Interferon, 10ml (8,000,000 unit), is developed by Institute of Microorganism, Academia Sinica.
Case is originated
In December, 2010, the nursery pig on multiple pig farm, the situations such as cluster generation dyspnea (ventral breathing) in various degree, spirit are depressed, drowsiness, inappetence, severe patient cough, asthma, respiratory frequency up to 80 times/more than min, the symptoms such as ear, abdominal part and leg cyanosis.Cut open the visible pulmonary congestion of the sick pig of inspection, occur edema, focal pneumonia; What have has the symptom such as hydrothorax, enlargement of hilar lymph nodes.Once used multiple Drug therapy as sulfadiazine clinically, penicillin and streptomycin etc. are showed no effect, and swinery carried out the immunity of swine fever, pig blue-ear disease and streptococcicosis.
Random sterile blood sampling 10 parts, separation of serum, with reference to soup scape unit report method (mixed infection [J] of porcine reproductive and respiratory syndrome virus and pathogenic salmonella. Chinese veterinary's journal, 2005 (7): 337-338.) RT-PCR that carries out PRRSV detects.
The extraction of pathological material of disease total serum IgE
Get serum 0.25ml and add 1ml Trizol reagent, incubated at room 5min, add 0.2ml chloroform, fully rock centrifuge tube 15s, incubated at room 2 ~ 3min, 4 DEG C, the centrifugal 15min of 12000r/min, transfer supernatant is in another new 1.5ml centrifuge tube, add 0.5ml isopropyl alcohol, fully rock, place 10min, 4 DEG C, the centrifugal 10min precipitate nucleic acids of 12000r/min, abandon supernatant, add 75% ethanol 1ml, jolt, washing nucleic acid, 4 DEG C, the centrifugal 5min of 8000r/min, abandon supernatant, drying at room temperature precipitation 5min, add 40 μ l DEPC water dissolution precipitate.
Reverse transcription
Get downstream primer GP5.2 0.5 μ l, 10mmol/l dNTPs0.25 μ l, 5 × reverse transcription buffer2.0 μ l and total serum IgE 7.0 μ l, 65 DEG C of water-bath 10min, then add M-MLV reverse transcription 0.25 μ l, mend to cumulative volume 10.0 μ l, 37 DEG C of water-bath 1h with aseptic double-distilled water.
PCR
Reaction cumulative volume 25 μ l.Forward primer GP5.1 0.5 μ l, downstream primer GP5.2 0.5 μ l, 2.5mmol/l dNTPs 2.0 μ l, 10 × Mg 2+freebuffer 2.5 μ l, 25mmol/l Mg 2+1.5 μ l, rTaq enzyme 2.5U, cDNA 1 μ l, mends to cumulative volume 25 μ l with sterilizing distilled water.Reaction cycle parameter: 94 DEG C of denaturation 5min; 94 DEG C of degeneration 45s, 59 DEG C of annealing 45s, 72 DEG C extend 60s, carry out 35 circulations altogether; Then 72 DEG C extend 10min.Get PCR primer and carry out 2% agarose gel electrophoresis.
RT-PCR testing result
10 parts of blood serum sample results are positive entirely.According to clinical symptoms, cut open inspection change and results of serological detection, being diagnosed as main pathogen is porcine reproductive and respiratory syndrome virus.
GP TH
Get disease pig 210 and be divided into 3 groups, put into 3 respectively and clean up and the isolating pig house of sterilizing.Chinese medicine fodder group (70), feedstuff prepared by the feeding embodiment of the present invention 4; Antibiotic matched group (30), gavages administration 10ml by Chinese medicinal compound extract stock solution;
Interferon group (30), injection of interferon 5ml; Antibiotic matched group
(30), every intramuscular injection cefotaxime sodium 1; Each group all above
Medication every day 2 times, is used in conjunction 5d.Blank group (10), not administration.
All above-mentioned this intellectual properties of primary enforcement, not setting restriction this new product of other forms of enforcement and/or new method.Those skilled in the art will utilize this important information, and foregoing is revised, to realize similar implementation status.But all modifications or transformation belong to the right of reservation based on new product of the present invention.
The above is only preferred embodiment of the present invention, and be not restriction the present invention being made to other form, any those skilled in the art may utilize the technology contents of above-mentioned announcement to be changed or be modified as the Equivalent embodiments of equivalent variations.But everyly do not depart from technical solution of the present invention content, any simple modification, equivalent variations and the remodeling done above embodiment according to technical spirit of the present invention, still belong to the protection domain of technical solution of the present invention.

Claims (6)

1. for preventing and treating a Chinese medicine composition for Porcine reproductive and respiratory syndrome, it is characterized in that: crude drug comprises Flos Lonicerae, Fructus Gardeniae, the Rhizoma Anemarrhenae, Commeline communis, Cortex Phellodendri, Radix Salviae Miltiorrhizae, Caulis Polygoni Multiflori, the Radix Astragali, Herba Artemisiae Annuae, Myrrha, Rhizoma Polygoni Cuspidati, Endothelium Corneum Gigeriae Galli, Herba Ecliptae, Radix Liriopes, Herba Scutellariae Barbatae, Radix Campanumoeae, Herba Hyperici Sampsonii, Herba Taxilli, Ramulus Cinnamomi, Radix Saposhnikoviae, Herba Centipedae, Rhizoma Dioscoreae, Radix Morindae Officinalis, Ramulus et Folium Bauhiniae Faberi, Hygrophila salicifolia, Rhizoma Picrorhizae and Radix Glycyrrhizae.
2. Chinese medicine composition as claimed in claim 1, is characterized in that: the weight of each crude drug is respectively Flos Lonicerae 55 weight portion ~ 65 weight portion, Fructus Gardeniae 30 weight portion ~ 40 weight portion, the Rhizoma Anemarrhenae 25 weight portion ~ 35 weight portion, Commeline communis 20 weight portion ~ 30 weight portion, Cortex Phellodendri 30 weight portion ~ 40 weight portion, Radix Salviae Miltiorrhizae 25 weight portion ~ 35 weight portion, Caulis Polygoni Multiflori 30 weight portion ~ 40 weight portion, the Radix Astragali 55 weight portion ~ 65 weight portion, Herba Artemisiae Annuae 25 weight portion ~ 35 weight portion, Myrrha 15 weight portion ~ 25 weight portion, Rhizoma Polygoni Cuspidati 20 weight portion ~ 30 weight portion, Endothelium Corneum Gigeriae Galli 30 weight portion ~ 40 weight portion, Herba Ecliptae 25 weight portion ~ 35 weight portion, Radix Liriopes 20 weight portion ~ 30 weight portion, Herba Scutellariae Barbatae 25 weight portion ~ 35 weight portion, Radix Campanumoeae 20 weight portion ~ 30 weight portion, Herba Hyperici Sampsonii 25 weight portion ~ 35 weight portion, Herba Taxilli 30 weight portion ~ 40 weight portion, Ramulus Cinnamomi 20 weight portion ~ 30 weight portion, Radix Saposhnikoviae 25 weight portion ~ 35 weight portion, Herba Centipedae 25 weight portion ~ 35 weight portion, Rhizoma Dioscoreae 20 weight portion ~ 30 weight portion, Radix Morindae Officinalis 20 weight portion ~ 30 weight portion, Ramulus et Folium Bauhiniae Faberi 25 weight portion ~ 35 weight portion, Hygrophila salicifolia 20 weight portion ~ 30 weight portion, Rhizoma Picrorhizae 15 weight portion ~ 25 weight portion and Radix Glycyrrhizae 20 weight portion ~ 30 weight portion.
3. Chinese medicine composition as claimed in claim 1 or 2, is characterized in that: the weight of each crude drug is respectively Flos Lonicerae 58 weight portion ~ 62 weight portion, Fructus Gardeniae 33 weight portion ~ 37 weight portion, the Rhizoma Anemarrhenae 28 weight portion ~ 32 weight portion, Commeline communis 23 weight portion ~ 27 weight portion, Cortex Phellodendri 33 weight portion ~ 37 weight portion, Radix Salviae Miltiorrhizae 28 weight portion ~ 32 weight portion, Caulis Polygoni Multiflori 33 weight portion ~ 37 weight portion, the Radix Astragali 58 weight portion ~ 62 weight portion, Herba Artemisiae Annuae 28 weight portion ~ 32 weight portion, Myrrha 18 weight portion ~ 22 weight portion, Rhizoma Polygoni Cuspidati 23 weight portion ~ 27 weight portion, Endothelium Corneum Gigeriae Galli 33 weight portion ~ 37 weight portion, Herba Ecliptae 28 weight portion ~ 32 weight portion, Radix Liriopes 23 weight portion ~ 27 weight portion, Herba Scutellariae Barbatae 28 weight portion ~ 32 weight portion, Radix Campanumoeae 23 weight portion ~ 27 weight portion, Herba Hyperici Sampsonii 28 weight portion ~ 32 weight portion, Herba Taxilli 33 weight portion ~ 37 weight portion, Ramulus Cinnamomi 23 weight portion ~ 27 weight portion, Radix Saposhnikoviae 28 weight portion ~ 32 weight portion, Herba Centipedae 28 weight portion ~ 32 weight portion, Rhizoma Dioscoreae 23 weight portion ~ 27 weight portion, Radix Morindae Officinalis 23 weight portion ~ 27 weight portion, Ramulus et Folium Bauhiniae Faberi 28 weight portion ~ 32 weight portion, Hygrophila salicifolia 23 weight portion ~ 27 weight portion, Rhizoma Picrorhizae 18 weight portion ~ 22 weight portion and Radix Glycyrrhizae 23 weight portion ~ 27 weight portion.
4. the Chinese medicine composition as described in claims 1 to 3, it is characterized in that: the weight of each crude drug is respectively Flos Lonicerae 60 weight portion, Fructus Gardeniae 35 weight portion, the Rhizoma Anemarrhenae 30 weight portion, Commeline communis 25 weight portion, Cortex Phellodendri 35 weight portion, Radix Salviae Miltiorrhizae 30 weight portion, Caulis Polygoni Multiflori 35 weight portion, the Radix Astragali 60 weight portion, Herba Artemisiae Annuae 30 weight portion, Myrrha 20 weight portion, Rhizoma Polygoni Cuspidati 25 weight portion, Endothelium Corneum Gigeriae Galli 35 weight portion, Herba Ecliptae 30 weight portion, Radix Liriopes 25 weight portion, Herba Scutellariae Barbatae 30 weight portion, Radix Campanumoeae 25 weight portion, Herba Hyperici Sampsonii 30 weight portion, Herba Taxilli 35 weight portion, Ramulus Cinnamomi 25 weight portion, Radix Saposhnikoviae 30 weight portion, Herba Centipedae 30 weight portion, Rhizoma Dioscoreae 25 weight portion, Radix Morindae Officinalis 25 weight portion, Ramulus et Folium Bauhiniae Faberi 30 weight portion, Hygrophila salicifolia 25 weight portion, Rhizoma Picrorhizae 20 weight portion and Radix Glycyrrhizae 25 weight portion.
5. the preparation method of Chinese medicine composition described in Claims 1-4, is characterized in that: mixed by weight by each raw material components, gets 100g mixture, add the water of 500g ~ 600g, boil 3 ~ 4 hours, filter, obtain the first filtrate, filtering residue adds the water of 200g ~ 300g again, again boils 1 ~ 2 hour, filter, obtain the second filtrate, by the first filtrate and the mixing of the second filtrate, concentrated removing aqueous solvent, pulverize, dry, obtain Chinese medicine composition.
6. for preventing and treating a Chinese medicine composition composition injection for Porcine reproductive and respiratory syndrome, it is characterized in that: the crude drug of described injection comprises Flos Lonicerae, Fructus Gardeniae, the Rhizoma Anemarrhenae, Commeline communis, Cortex Phellodendri, Radix Salviae Miltiorrhizae, Caulis Polygoni Multiflori, the Radix Astragali, Herba Artemisiae Annuae, Myrrha, Rhizoma Polygoni Cuspidati, Endothelium Corneum Gigeriae Galli, Herba Ecliptae, Radix Liriopes, Herba Scutellariae Barbatae, Radix Campanumoeae, Herba Hyperici Sampsonii, Herba Taxilli, Ramulus Cinnamomi, Radix Saposhnikoviae, Herba Centipedae, Rhizoma Dioscoreae, Radix Morindae Officinalis, Ramulus et Folium Bauhiniae Faberi, Hygrophila salicifolia, Rhizoma Picrorhizae and Radix Glycyrrhizae;
The weight of each crude drug is respectively Flos Lonicerae 55 weight portion ~ 65 weight portion, Fructus Gardeniae 30 weight portion ~ 40 weight portion, the Rhizoma Anemarrhenae 25 weight portion ~ 35 weight portion, Commeline communis 20 weight portion ~ 30 weight portion, Cortex Phellodendri 30 weight portion ~ 40 weight portion, Radix Salviae Miltiorrhizae 25 weight portion ~ 35 weight portion, Caulis Polygoni Multiflori 30 weight portion ~ 40 weight portion, the Radix Astragali 55 weight portion ~ 65 weight portion, Herba Artemisiae Annuae 25 weight portion ~ 35 weight portion, Myrrha 15 weight portion ~ 25 weight portion, Rhizoma Polygoni Cuspidati 20 weight portion ~ 30 weight portion, Endothelium Corneum Gigeriae Galli 30 weight portion ~ 40 weight portion, Herba Ecliptae 25 weight portion ~ 35 weight portion, Radix Liriopes 20 weight portion ~ 30 weight portion, Herba Scutellariae Barbatae 25 weight portion ~ 35 weight portion, Radix Campanumoeae 20 weight portion ~ 30 weight portion, Herba Hyperici Sampsonii 25 weight portion ~ 35 weight portion, Herba Taxilli 30 weight portion ~ 40 weight portion, Ramulus Cinnamomi 20 weight portion ~ 30 weight portion, Radix Saposhnikoviae 25 weight portion ~ 35 weight portion, Herba Centipedae 25 weight portion ~ 35 weight portion, Rhizoma Dioscoreae 20 weight portion ~ 30 weight portion, Radix Morindae Officinalis 20 weight portion ~ 30 weight portion, Ramulus et Folium Bauhiniae Faberi 25 weight portion ~ 35 weight portion, Hygrophila salicifolia 20 weight portion ~ 30 weight portion, Rhizoma Picrorhizae 15 weight portion ~ 25 weight portion and Radix Glycyrrhizae 20 weight portion ~ 30 weight portion,
The preparation method of described injection is specially,
Each raw material components is mixed by weight, gets 100g mixture, add the water of 500g ~ 600g, boil 3 ~ 4 hours, filter, obtain the first filtrate, filtering residue adds the water of 200g ~ 300g again, again boils 1 ~ 2 hour, filter, obtain the second filtrate, by the first filtrate and the mixing of the second filtrate, concentrated removing aqueous solvent, pulverize, dry, obtain Chinese medicine composition, described Chinese medicine composition is dissolved in water, adjust pH 7.0, filters, embedding, sterilizing, obtaining crude drug concentration is the injection of 1g/ml.
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Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104800300A (en) * 2015-05-14 2015-07-29 四川铁骑力士实业有限公司 Traditional Chinese medicine composition for preventing porcine reproductive and respiratory syndrome and preparation method thereof
CN105053741A (en) * 2015-09-23 2015-11-18 青岛华仁技术孵化器有限公司 Feed capable of preventing and treating porcine reproductive and respiratory syndrome
CN105213918A (en) * 2015-09-23 2016-01-06 青岛华仁技术孵化器有限公司 The immune formulation of control Porcine reproductive and respiratory syndrome
CN105535452A (en) * 2015-12-28 2016-05-04 青岛华仁技术孵化器有限公司 Medicine for preventing and treating porcine reproductive and respiratory syndrome
CN109663088A (en) * 2019-02-21 2019-04-23 郑州赛科药业科技有限公司 A kind of Chinese medicine composition and preparation method thereof for preventing and treating porcine reproductive and respiratory syndrome
CN109700927A (en) * 2019-02-21 2019-05-03 郑州赛科药业科技有限公司 It prevents and treats poison and invades Chinese medicine composition and preparation method that lung defends type porcine reproductive and respiratory syndrome
CN116173002A (en) * 2023-03-02 2023-05-30 河南农业大学 Application of salvianolic acid A in preparation of medicines for preventing and treating porcine reproductive and respiratory syndrome

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101391057A (en) * 2008-10-23 2009-03-25 田明军 Medicine for preventing and treating porcine reproductive and respiratory syndrome
CN102078419A (en) * 2010-12-24 2011-06-01 任先武 Medicament for preventing and treating porcine reproductive and respiratory syndrome (PRRS) and preparation method thereof
CN103054999A (en) * 2013-01-31 2013-04-24 山东方舟生物科技有限公司 Traditional Chinese medicine preparation for preventing and treating highly pathogenic porcine reproductive and respiratory syndrome and preparation method

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101391057A (en) * 2008-10-23 2009-03-25 田明军 Medicine for preventing and treating porcine reproductive and respiratory syndrome
CN102078419A (en) * 2010-12-24 2011-06-01 任先武 Medicament for preventing and treating porcine reproductive and respiratory syndrome (PRRS) and preparation method thereof
CN103054999A (en) * 2013-01-31 2013-04-24 山东方舟生物科技有限公司 Traditional Chinese medicine preparation for preventing and treating highly pathogenic porcine reproductive and respiratory syndrome and preparation method

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
张谈顺: "猪蓝耳病的预防和治疗", 《畜牧兽医科技信息》 *

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104800300A (en) * 2015-05-14 2015-07-29 四川铁骑力士实业有限公司 Traditional Chinese medicine composition for preventing porcine reproductive and respiratory syndrome and preparation method thereof
CN104800300B (en) * 2015-05-14 2017-09-19 四川铁骑力士实业有限公司 It is a kind of to prevent Chinese medicine composition of pig blue-ear disease and preparation method thereof
CN105053741A (en) * 2015-09-23 2015-11-18 青岛华仁技术孵化器有限公司 Feed capable of preventing and treating porcine reproductive and respiratory syndrome
CN105213918A (en) * 2015-09-23 2016-01-06 青岛华仁技术孵化器有限公司 The immune formulation of control Porcine reproductive and respiratory syndrome
CN105535452A (en) * 2015-12-28 2016-05-04 青岛华仁技术孵化器有限公司 Medicine for preventing and treating porcine reproductive and respiratory syndrome
CN109663088A (en) * 2019-02-21 2019-04-23 郑州赛科药业科技有限公司 A kind of Chinese medicine composition and preparation method thereof for preventing and treating porcine reproductive and respiratory syndrome
CN109700927A (en) * 2019-02-21 2019-05-03 郑州赛科药业科技有限公司 It prevents and treats poison and invades Chinese medicine composition and preparation method that lung defends type porcine reproductive and respiratory syndrome
CN116173002A (en) * 2023-03-02 2023-05-30 河南农业大学 Application of salvianolic acid A in preparation of medicines for preventing and treating porcine reproductive and respiratory syndrome
CN116173002B (en) * 2023-03-02 2023-08-25 河南农业大学 Application of salvianolic acid A in preparation of medicines for preventing and treating porcine reproductive and respiratory syndrome

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