CN104258139A - Pharmaceutical composition for preventing and treating chronic swine plague and preparation method of pharmaceutical composition - Google Patents

Abstract

The invention discloses a pharmaceutical composition for preventing and treating chronic swine plague and a preparation method of the pharmaceutical composition. The pharmaceutical composition comprises the following raw Chinese medical materials: gypsum rubrum, jasmine, dried radix rehmanniae, cacumen biotae, moutan bark, rhizoma phragmitis, radices trichosanthis, lophatherum gracile, dayflower, phoenix-tail fern, polygonum aviculare, dandelion, lotus leaf, lotus plumule, radix ophiopogonis, fructus forsythia, radix scrophulariae, liquorice, Chinese eriosema root, dicliptera chinensis, bryoniifolia, adenophora trachelioides maxim., Indian melothria root, cynanchum thesioides, trichosanthes kirilowii maxim, tuberous sword fern tuber, giant clam meat and yam rhizome. The pharmaceutical composition mainly takes effects of clearing away heat and toxic materials, cooling blood and promoting the secretion of body fluid and also has auxiliary functions of invigorating spleen and supplementing qi, so as to reconcile yin and yang, and qi and blood of pigs with chronic swine plague to finally treat swine plague; the pharmaceutical composition for preventing and treating chronic swine plague has the advantages of short treatment course, relatively good effect, low residual quantity and good environment friendliness and simultaneously is capable of enhancing the immunity and the disease resistance of pigs.

Description

A kind of pharmaceutical composition for preventing and treating chronic type swine fever epidemic disease and preparation method thereof

Technical field

The present invention relates to field of veterinary, particularly relating to a kind of pharmaceutical composition for preventing and treating chronic type swine fever epidemic disease and preparation method thereof.

Background technology

Swine fever is a kind of hyperinfection and lethal epidemic disease that are caused by swine fever virus, it is characterized by high heat and delays extensively hemorrhage, the infraction that causes with thin vessels degeneration and downright bad.The pig premunition of infected pigs's Pestivirus reduces, very easily concurrent or secondary pig lung plague.Especially cold, moist, cold and heat succeed each other, weather drastic change, under the bad condition such as crowded, improper ventilation, pig body resistance reduced, pasteurellosis bacillus amount reproduction can be caused, virulence enhancing and falling ill, as controlled not in time, the large quantities of death of pig can be caused.

The sick pig spirit of clinical symptoms is depressed, and body temperature is elevated to 40 DEG C ~ 41.5 DEG C.In continued fever, loss of appetite is even absolutely useless, and eye flows dense sexual secretion.Visual mucosa cyanosis.Sick just constipation, after have loose bowels, stench band blood is also mixed with white mucosa.Whole body is rubescent, and have sharp ears, abdominal part, cervical region and limbs skin have purpura, and finger pressure does not fade.Boar foreskin has muddy urine, and neck swelling is hard, the sensitivity touched.Sick pig cough, dyspnea, calls hoarse, and nose flows out serosity or sticky secretions, indivedual hog snout holes elution band bleeding blister foam, and serious is the mouth breathing of dog sitting posture, eventually because of death by suffocation.Inspection sick dead pig is cutd open in pathological change altogether, pathological change basic simlarity, and lymph nodes of body as a whole enlargement is hemorrhage, in kermesinus; Stomach bottom mucosa, bladder mucosa are hemorrhage; Spleen surface has the hemorrhage infarction of the hemorrhage and Semen phaseoli radiati size mulberry of mound shape point, and there is needle point massive hemorrhage point on surface; Ileocecal valve, caecum and colonic mucosa have button-type ulcer; Larynx has ecchymosis and petechia; Heart hat fat, endocardium have petechia; Pulmonary edema is furvous, and has the pneumonia stove not of uniform size be dispersed in; Have mucus blister secretions in trachea-bronchial epithelial cell, and be mixed with quantity not wait courageous and upright foam; Thoracic cavity and pericardium amass the pink turbid liquid of volume; Cervical region subcutaneous dropsy, flush, congestion.

Pestilence is early on the books in Chinese historical data.As " all gift heavenly officials tombs are killed " is recorded: " physician slaps the disease of foster ten thousand people, all has pestilence disease when four." " Springs and Autumns of Master Lü last month of spring discipline " record: " summer weather in spring in season, the then people's many diseases epidemic disease." illustrate and at that time certain level is reached to the understanding of pestilence, think that pestilence all can occur throughout the year, one of reason is gas abnormal due to season, is caused by " gas time non-".The Chinese medical book Huangdi's Internal Classics of earliest extant is also on the books.As " Plain Questions acupuncture manipulation opinion " points out: " five types of pestilence extremely, all to dye easily, without asking size, condition of illness is similar ..., in healthy energy is deposited, heresy can not be done, preventing the invasion of infectious pathogen." " Plain Questions instinct sick " section: " and the moon of fainting is not given up the throne, and namely strong wind is early lifted, time rain do not fall, wet order is not changed, people's suffering from epidemic febrile disease epidemic disease, and defect gives up.Humorously, people's disease is arthrodynia of the extremities, head's pain all, latent heat restlessness, the dry taking fluids profusely of throat." point out that pestilence has the features such as infectiousness, popularity, clinical manifestation are similar, morbidity is relevant with weather, as long as and think " healthy energy deposit in ", with regard to energy " preventing the invasion of infectious pathogen ".

Adopt Western medicine to prevent and treat this disease and mostly adopt antibiotic, occurred and had increasing trend to the bacterial strain that these medicines develop immunity to drugs, in addition, these chemicalses obviously can cause pig drug disposition to remain, and then affect human and livestock health and even endanger public health.Western medical treatment chronic type swine fever epidemic disease course for the treatment of is longer, the undesirable epidemic situation of effect is wayward, can cause very large economic loss.

But China's Chinese medicine and pharmacy just had understanding to pestilence (infectious disease) before 2 over thousands of years, and propose reasonably to take precautions against and treatment, as the Zhou Dynasty " Book of Rites. month " record " the row autumn first month of spring is the sick large epidemic disease of the people then ", " the asking that size condition of illness is similar to nothing to all passing on from one to another of five types of pestilence " is had clearly to propose infectiousness and the epidemic characteristic of epidemic disease in Huangdi's Internal Classics, the method of Herba Artemisiae Annuae treating malaria disease is the medicine dedicated of malaria in the world by generally acknowledging in the world so far in addition, in conjunction with in current " swine flu " control, health ministry clearly proposes Chinese medicine and participates in control prece and more demonstrate China's Chinese Traditional Medicine and preventing and treating the importance and functions in pestilence.

The incubation period of natural infection swine fever is generally 5-7 d, and that short is 2 d, and long reaches 21 d.According to the state of an illness and the course of disease, often in clinical symptoms swine fever is divided into most acute, acute, chronic type and delayed.

1, most acute is more common in the popular initial stage, sudden onset, and symptom sharply, performance general spasticity, tic of the limbs, delay, skin and mucosa cyanosis, has hemorrhage speckle, dead through 1-8 days by high heat.2, acute.This kind of is the most common.In sick pig before there is symptom, namely body temperature raised at 41 degrees centigrade, persistent, show as slow in action, end to end sagging, hogback, shiver, thirsty, a normal sleeping place is drowsiness, the inflammation of eye conjunctiva, the puffiness of the eyes, secretions increase, and can see purple red color spot point at places such as hypogastric region, the basal part of the ear, four hoof, lip, pudendums.Sick difficulty of just discharging feces, occur diarrhoea soon, feces is lark.Boar foreskin inner product has urine, flows out muddy canescence stench liquid with after hands extruding.Also can there is acute swine fever in suckling pig, main manifestations nervous symptoms, as ground one's teeth in sleep, spasm, opisthotonus or the tic that falls down to the ground, finally dead.3, chronic type.Main manifestations is become thin, anemia, and asthenia universalis is often lain prostrate, and gait is slowly unable, inappetence, and constipation and diarrhoea are alternately.The sick pig had has purpura or downright bad crust on ear end, tail point and limbs skin.The course of disease more than one month.Died long term development is not bad becomes cad pig.Virus may be passed to fetus by Placenta Hominis, cause miscarriage after infecting by in-pig, occurs diarrhoea after producing stillborn fetus or the small and weak piglet of output or wean.4, mild.PD is slow, sick temperature of pig body generally at 40-41 degrees Celsius, skin often without hemorrhage point, but in the common blood stasis of hypogastrium and necrosis.Sometimes visible ear and tail skin necrosis, paddy claims dry ear, dry tail.The course of disease reaches 2-3 months.

Summary of the invention

Technical problem to be solved by this invention is to provide one to have can heat-clearing and toxic substances removing, removing heat from blood promote the production of body fluid, treatment chronic type swine fever epidemic disease, pig yang blood and qi can be made to be in harmonious proportion, treatment short treating period, effect is better, residual quantity low while can strengthen the immunity of pig and see resistance against diseases, the Chinese medicine of green non-pollution.

In order to realize foregoing invention object, the invention provides a kind of pharmaceutical composition for preventing and treating chronic type swine fever epidemic disease, described pharmaceutical composition comprises following raw medicinal material: Mirabilitum crystallina, Fructus Gardeniae, the Radix Rehmanniae, Cacumen Platycladi, Cortex Moutan, Rhizoma Phragmitis, Radix Trichosanthis, Herba Lophatheri, Herba Commelinae, Herba Pteridis Multifidae, Herba Polygoni Avicularis, Herba Taraxaci, Folium Nelumbinis, Plumula Nelumbinis, Radix Ophiopogonis, Fructus Forsythiae, Radix Scrophulariae, Radix Glycyrrhizae, Radix eriosematis chinensis, Herba Diclipterae Chinensis, Caulis et folium vitis thunbergii, Radix Adenophorae Trachelioidis, Radix Melothriae Heterophyllae, Herba Cynanchi Thesioidis, Fructus Trichosanthis, Rhizoma Nephrolepis cordifoliae, Concha Tridacna squamosa meat are with Rhizoma Dioscoreae Septemlobae.

In described pharmaceutical composition, the parts by weight of each raw medicinal material are: Mirabilitum crystallina 23-36 part, Fructus Gardeniae 7-15 part, Radix Rehmanniae 17-35 part, Cacumen Platycladi 25-40 part, Cortex Moutan 9-25 part, Rhizoma Phragmitis 13-27 part, Radix Trichosanthis 10-28 part, Herba Lophatheri 25-38 part, Herba Commelinae 28-40 part, Herba Pteridis Multifidae 26-39 part, Herba Polygoni Avicularis 12-32 part, Herba Taraxaci 12-37 part, Folium Nelumbinis 20-35 part, Plumula Nelumbinis 15-28 part, Radix Ophiopogonis 15-30 part, Fructus Forsythiae 12-25 part, Radix Scrophulariae 25-40 part, Radix Glycyrrhizae 15-32 part, Radix eriosematis chinensis 11-23 part, Herba Diclipterae Chinensis 17-30 part, Caulis et folium vitis thunbergii 26-42 part, Radix Adenophorae Trachelioidis 3-10 part, Radix Melothriae Heterophyllae 15-29 part, Herba Cynanchi Thesioidis 20-37 part, Fructus Trichosanthis 7-23 part, Rhizoma Nephrolepis cordifoliae 11-29 part, Concha Tridacna squamosa meat 20-38 part is with Rhizoma Dioscoreae Septemlobae 14-29 part.

In described pharmaceutical composition, the parts by weight of each raw medicinal material are: Mirabilitum crystallina 33 parts, Fructus Gardeniae 11 parts, 24 parts, the Radix Rehmanniae, Cacumen Platycladi 36 parts, Cortex Moutan 19 parts, Rhizoma Phragmitis 21 parts, Radix Trichosanthis 12 parts, Herba Lophatheri 35 parts, Herba Commelinae 29 parts, Herba Pteridis Multifidae 38 parts, Herba Polygoni Avicularis 14 parts, Herba Taraxaci 25 parts, 32 parts, Folium Nelumbinis, Plumula Nelumbinis 17 parts, Radix Ophiopogonis 15 parts, Fructus Forsythiae 16 parts, Radix Scrophulariae 39 parts, 32 parts, Radix Glycyrrhizae, Radix eriosematis chinensis 11 parts, Herba Diclipterae Chinensis 30 parts, Caulis et folium vitis thunbergii 40 parts, Radix Adenophorae Trachelioidis 5 parts, Radix Melothriae Heterophyllae 17 parts, Herba Cynanchi Thesioidis 24 parts, Fructus Trichosanthis 8 parts, Rhizoma Nephrolepis cordifoliae 17 parts, Concha Tridacna squamosa meat 35 parts of He Rhizoma Dioscoreae Septemlobae 16 parts.

Described pharmaceutical composition also comprises Herba Ecliptae, Semen Coicis, Herba Moslae, Fructus Rubi and Semen Hoveniae (Fructus Hoveniae).

In described pharmaceutical composition, the parts by weight of each raw medicinal material are: Mirabilitum crystallina 23-36 part, Fructus Gardeniae 7-15 part, Radix Rehmanniae 17-35 part, Cacumen Platycladi 25-40 part, Cortex Moutan 9-25 part, Rhizoma Phragmitis 13-27 part, Radix Trichosanthis 10-28 part, Herba Lophatheri 25-38 part, Herba Commelinae 28-40 part, Herba Pteridis Multifidae 26-39 part, Herba Polygoni Avicularis 12-32 part, Herba Taraxaci 12-37 part, Folium Nelumbinis 20-35 part, Plumula Nelumbinis 15-28 part, Radix Ophiopogonis 15-30 part, Fructus Forsythiae 12-25 part, Radix Scrophulariae 25-40 part, Radix Glycyrrhizae 15-32 part, Radix eriosematis chinensis 11-23 part, Herba Diclipterae Chinensis 17-30 part, Caulis et folium vitis thunbergii 26-42 part, Radix Adenophorae Trachelioidis 3-10 part, Radix Melothriae Heterophyllae 15-29 part, Herba Cynanchi Thesioidis 20-37 part, Fructus Trichosanthis 7-23 part, Rhizoma Nephrolepis cordifoliae 11-29 part, Concha Tridacna squamosa meat 20-38 part, Rhizoma Dioscoreae Septemlobae 14-29 part, Herba Ecliptae 3-9 part, Semen Coicis 12-19 part, Herba Moslae 4-8 part, Fructus Rubi 5-11 part and Semen Hoveniae (Fructus Hoveniae) 7-12 part.

In described pharmaceutical composition, the parts by weight of each raw medicinal material are: Mirabilitum crystallina 33 parts, Fructus Gardeniae 11 parts, 24 parts, the Radix Rehmanniae, Cacumen Platycladi 36 parts, Cortex Moutan 19 parts, Rhizoma Phragmitis 21 parts, Radix Trichosanthis 12 parts, Herba Lophatheri 35 parts, Herba Commelinae 29 parts, Herba Pteridis Multifidae 38 parts, Herba Polygoni Avicularis 14 parts, Herba Taraxaci 25 parts, 32 parts, Folium Nelumbinis, Plumula Nelumbinis 17 parts, Radix Ophiopogonis 15 parts, Fructus Forsythiae 16 parts, Radix Scrophulariae 39 parts, 32 parts, Radix Glycyrrhizae, Radix eriosematis chinensis 11 parts, Herba Diclipterae Chinensis 30 parts, Caulis et folium vitis thunbergii 40 parts, Radix Adenophorae Trachelioidis 5 parts, Radix Melothriae Heterophyllae 17 parts, Herba Cynanchi Thesioidis 24 parts, Fructus Trichosanthis 8 parts, Rhizoma Nephrolepis cordifoliae 17 parts, Concha Tridacna squamosa meat 35 parts, Rhizoma Dioscoreae Septemlobae 16 parts, Herba Ecliptae 4 parts, Semen Coicis 17 parts, Herba Moslae 5 parts, Fructus Rubi 8 parts and Semen Hoveniae (Fructus Hoveniae) 10 parts.

The dosage form of described pharmaceutical composition is capsule, and its preparation method comprises the following steps:

The first step, Mirabilitum crystallina, Fructus Gardeniae, the Radix Rehmanniae, Cacumen Platycladi, Cortex Moutan, Rhizoma Phragmitis, Radix Trichosanthis and Herba Lophatheri are mixed in described ratio, add the water extraction volatile oil relative to 4 ~ 6 times, mixture, by the volatile oil beta-cyclodextrin inclusion compound obtained, the mass ratio of volatile oil and beta-schardinger dextrin-is 1: 3 ~ 6, stirs 60 minutes ~ 90 minutes, temperature 30 DEG C ~ 50 DEG C, obtain volatile oil clathrate compound, for subsequent use; Another when aqueous extract being concentrated into 60 DEG C relative density be the clear paste of 1.15 ~ 1.17;

Second step, Herba Commelinae, Herba Pteridis Multifidae, Herba Polygoni Avicularis, Herba Taraxaci and Folium Nelumbinis are mixed in described ratio, add relative to mixture 5 ~ 8 times amount determining alcohol be 75% ~ 90% alcohol reflux 3 ~ 5 times, each 2 ~ 3 hours, filter respectively, each time filtrate merges, and reclaims ethanol, when being concentrated into 60 DEG C, relative density is the clear paste of 1.20 ~ 1.22, for subsequent use;

3rd step, will remain described raw medicinal material and mix in described ratio, add relative to mixture 8 ~ 10 times of water gagings, boil 6 ~ 8 hours, filter, and when being concentrated into 60 DEG C, relative density is the clear paste of 1.28 ~ 1.30;

4th step, mixed by the clear paste of the first step, second step, the 3rd step, 60 DEG C ~ 70 DEG C reduced vacuum dryings, get dry extract powder;

5th step, dried cream powder described in volatile oil clathrate compound described in the first step and the 4th step is fully mixed, add the mixture relative to volatile oil clathrate compound described in 100 weight portions and dried cream powder subsequently, 5 ~ 7 weight portion carboxymethyl starch sodium, 5 ~ 50 weight starch mix homogeneously, adding 150 ~ 200 weight portion determining alcohols is 80% ~ 90% alcohol granulation, dry, granulate, add the mixture relative to volatile oil clathrate compound described in 100 weight portions and dried cream powder subsequently, 3 ~ 5 weight portion magnesium stearate, then load in gelatine capsule.

The dosage form of described pharmaceutical composition is powder, and its preparation method comprises the following steps:

Step one, all crude drug are dropped in proportion in blender stir, mixing, pulverize in rear feeding pulverizer, the Chinese medicine composition after pulverizing is loaded in multi-function extractor, add relative to its quality 5-7 times amount water, boil 2-4 hour, steam pressure is 0.6-1Mpa process, filter, obtain the first filtrate and filtering residue;

Step 2, the filtering residue of acquisition added again relative to its quality 5-7 times amount water, boil 2-4 hour, steam pressure is 0.6Mpa-1Mpa process, filter and obtain the second filtrate and filtering residue;

Step 3, the first filtrate and the second filtrate merged, concentrated removing moisture, obtains and gets dry extract, and adopts super-fine powder mill to pulverize, is crushed to less than 30 μm, obtain pharmaceutical composition powder.

In order to realize goal of the invention better, the invention provides a kind of preparation method of the pharmaceutical composition for preventing and treating chronic type swine fever epidemic disease, the dosage form of described pharmaceutical composition is capsule, and its preparation method comprises the following steps:

The first step, Mirabilitum crystallina, Fructus Gardeniae, the Radix Rehmanniae, Cacumen Platycladi, Cortex Moutan, Rhizoma Phragmitis, Radix Trichosanthis and Herba Lophatheri are mixed in described ratio, add the water extraction volatile oil relative to 4 ~ 6 times, mixture, by the volatile oil beta-cyclodextrin inclusion compound obtained, the mass ratio of volatile oil and beta-schardinger dextrin-is 1: 3 ~ 6, stirs 60 minutes ~ 90 minutes, temperature 30 DEG C ~ 50 DEG C, obtain volatile oil clathrate compound, for subsequent use; Another when aqueous extract being concentrated into 60 DEG C relative density be the clear paste of 1.15 ~ 1.17;

Second step, Herba Commelinae, Herba Pteridis Multifidae, Herba Polygoni Avicularis, Herba Taraxaci and Folium Nelumbinis are mixed in described ratio, add relative to mixture 5 ~ 8 times amount determining alcohol be 75% ~ 90% alcohol reflux 3 ~ 5 times, each 2 ~ 3 hours, filter respectively, each time filtrate merges, and reclaims ethanol, when being concentrated into 60 DEG C, relative density is the clear paste of 1.20 ~ 1.22, for subsequent use;

3rd step, will remain described raw medicinal material and mix in described ratio, add relative to mixture 8 ~ 10 times of water gagings, boil 6 ~ 8 hours, filter, and when being concentrated into 60 DEG C, relative density is the clear paste of 1.28 ~ 1.30;

4th step, mixed by the clear paste of the first step, second step, the 3rd step, 60 DEG C ~ 70 DEG C reduced vacuum dryings, get dry extract powder;

5th step, dried cream powder described in volatile oil clathrate compound described in the first step and the 4th step is fully mixed, add the mixture relative to volatile oil clathrate compound described in 100 weight portions and dried cream powder subsequently, 5 ~ 7 weight portion carboxymethyl starch sodium, 5 ~ 50 weight starch mix homogeneously, adding 150 ~ 200 weight portion determining alcohols is 80% ~ 90% alcohol granulation, dry, granulate, add the mixture relative to volatile oil clathrate compound described in 100 weight portions and dried cream powder subsequently, 3 ~ 5 weight portion magnesium stearate, then load in gelatine capsule.

In order to realize goal of the invention better, the invention provides a kind of preparation method of the pharmaceutical composition for preventing and treating chronic type swine fever epidemic disease, the dosage form of described pharmaceutical composition is powder, and its preparation method comprises the following steps:

Step one, all crude drug are dropped in proportion in blender stir, mixing, pulverize in rear feeding pulverizer, the Chinese medicine composition after pulverizing is loaded in multi-function extractor, add relative to its quality 5-7 times amount water, boil 2-4 hour, steam pressure is 0.6-1Mpa process, filter, obtain the first filtrate and filtering residue;

Step 2, the filtering residue of acquisition added again relative to its quality 5-7 times amount water, boil 2-4 hour, steam pressure is 0.6Mpa-1Mpa process, filter and obtain the second filtrate and filtering residue;

Step 3, the first filtrate and the second filtrate merged, concentrated removing moisture, obtains and gets dry extract, and adopts super-fine powder mill to pulverize, is crushed to less than 30 μm, obtain pharmaceutical composition powder.

The invention has the beneficial effects as follows: the treatment of the pig suffering from chronic type swine fever epidemic disease is promoted the production of body fluid based on heat-clearing and toxic substances removing, removing heat from blood, be auxiliary with invigorating the spleen and benefiting QI, pig yang blood and qi is in harmonious proportion, treatment short treating period, effect is better, residual quantity low while can strengthen the immunity of pig and see resistance against diseases, green non-pollution.

Detailed description of the invention

For the corresponding symptom of pestilence that ill pig occurs, the invention provides a kind of mediation with scalable pig yang blood and qi, treatment short treating period, effect is better, immunity and the resistance against diseases of pig can be strengthened while residual quantity is low, the pharmaceutical composition of green non-pollution, comprise following raw medicinal material: Mirabilitum crystallina, Fructus Gardeniae, the Radix Rehmanniae, Cacumen Platycladi, Cortex Moutan, Rhizoma Phragmitis, Radix Trichosanthis, Herba Lophatheri, Herba Commelinae, Herba Pteridis Multifidae, Herba Polygoni Avicularis, Herba Taraxaci, Folium Nelumbinis, Plumula Nelumbinis, Radix Ophiopogonis, Fructus Forsythiae, Radix Scrophulariae, Radix Glycyrrhizae, Radix eriosematis chinensis, Herba Diclipterae Chinensis, Caulis et folium vitis thunbergii, Radix Adenophorae Trachelioidis, Radix Melothriae Heterophyllae, Herba Cynanchi Thesioidis, Fructus Trichosanthis, Rhizoma Nephrolepis cordifoliae, Concha Tridacna squamosa meat, Rhizoma Dioscoreae Septemlobae, Herba Ecliptae, Semen Coicis, Herba Moslae, Fructus Rubi and Semen Hoveniae (Fructus Hoveniae).This compatibility of medicines in a prescription is reasonable, rigorous, promotes the production of body fluid for Therapeutic Principle treats chronic type swine fever epidemic disease, can obtain desirable effect with heat-clearing and toxic substances removing, removing heat from blood.Mirabilitum crystallina clearing away heat-fire in we; Fructus Gardeniae, Radix eriosematis chinensis, Herba Diclipterae Chinensis, Caulis et folium vitis thunbergii, Radix Adenophorae Trachelioidis, Radix Melothriae Heterophyllae, Herba Cynanchi Thesioidis, Fructus Trichosanthis, Rhizoma Nephrolepis cordifoliae, Concha Tridacna squamosa meat with Rhizoma Dioscoreae Septemlobae, with the Radix Rehmanniae, Radix Scrophulariae, Cacumen Platycladi, Cortex Moutan can cooling blood for hemostasis, heat-clearing and toxic substances removing; Radix Trichosanthis, Radix Ophiopogonis, Fructus Forsythiae, Herba Taraxaci clearing away heat and promoting production of body fluid, detumescence and apocenosis; Herba Lophatheri, the Radix Rehmanniae, Radix Glycyrrhizae, Rhizoma Phragmitis etc. are for clearing away heart-fire; Herba Commelinae one medicine, is longer than heat clearing away, again can diuresis, and Herba Pteridis Multifidae, Herba Polygoni Avicularis, Herba Taraxaci are equivalent to be used.Wherein, Herba Commelinae and Herba Lophatheri can clearing away heat and promoting diuresis, and effect is similar, but the effect of Herba Commelinae is stronger.Folium Nelumbinis this product bitter in the mouth is put down, and its gas is fragrant clearly, can clearing away summer-heat, can rise again and send out spleen sun, hemostasis; Plumula Nelumbinis is bitter, cold, enters heart channel; Herba Ecliptae, Semen Coicis, Herba Moslae, Fructus Rubi and Semen Hoveniae (Fructus Hoveniae) invigorating the spleen and benefiting QI.

The pharmacological property of above-mentioned each raw medicinal material is as follows:

Mirabilitum crystallina: pungent, salty, cold; GUIXIN, stomach, kidney channel.Row calentura when curing mainly, high fever excessive thirst, laryngopharynx swelling and pain, edema, urine retention, aphtha of the mouth and tongue, carbuncle, erysipelas, scalds.

Fructus Gardeniae: bitter, cold, nontoxic; GUIXIN warp, Liver Channel, lung meridian, stomach warp, tri-jiao channel.Cure mainly the hot swelling pain of order, the large heat of breast heart intestine and small intestine, unhappy in the heart, pathogenic heat in the stomach gas.Pathogenic fire purging relieving restlessness, clearing away heat-damp and promoting diuresis, removing pathogenic heat from blood and toxic substance from the body, cooling blood for hemostasis.Calentura is vexed, liver-fire conjunctival congestion, and headache, jaundice due to damp-heat, stranguria, dysentery hematuria, aphtha of the mouth and tongue, sore swollen toxin, sprains and swell and ache.

The Radix Rehmanniae: sweet, cold; GUIXIN, liver, kidney channel.Heat clearing away, promote the production of body fluid, moisturize, laxation, removing blood stasis with potent drugs, tissue regeneration promoting, pain relieving, regulating menstruation, removing heat from blood, hemostasis invigorate blood circulation.

Cacumen Platycladi: bitter, puckery, cold.Return lung, liver, spleen channel.Cooling blood for hemostasis, growing and blacking hair.For hematemesis and epistaxis, spitting of blood, has blood in stool, metrostaxis, heat in blood alopecia, early whitening of beard and hair.

Cortex Moutan: bitter in the mouth, pungent, cold nature, GUIXIN, liver, kidney channel.There is the effect of clearing away heat and cooling blood, promoting blood circulation to remove blood stasis.

Rhizoma Phragmitis: sweet, cold; Return lung meridian, stomach warp.Clearing away heat and promoting production of body fluid, relieving restlessness, preventing or arresting vomiting, diuresis.Cure mainly: for calentura excessive thirst, gastropyretic vomiting, cough due to lung-heat, lung abscess vomiting pus, the puckery pain of pyretic stranguria.Rhizoma Phragmitis is used for gastropyretic vomiting.

Radix Trichosanthis: sweet, micro-hardship, is slightly cold; Attach to the lung and stomach meridians.Clearing away heat and promoting production of body fluid, detumescence and apocenosis.For calentura excessive thirst, lung-heat type cough, interior-heat is quenched one's thirst, sore swollen toxin.

Herba Lophatheri: sweet, light, cold.GUIXIN, stomach, small intestine meridian.Clearing heat and relieving fidgetness, diuresis.For calentura excessive thirst, hot urination drenches pain, aphtha of the mouth and tongue.

Herba Commelinae: sweet, cold, light; GUIXIN, all warps of liver,spleen,kidney, intestine and small intestine.Main cold and heat subtertian malaria, phlegm retention, fourth swells, and meat disease is not smooth, infantile erysipelas, mad epilepsy of generating heat, large abdomen feeling of fullness, body face edema due to disorder of QI, and hematodiarrhoea, dog stings Serpentis, the poison such as carbuncle.For fever caused by exogenous pathogens, or febrile disease heating is not moved back, or laryngopharynx swelling and pain, and the disease such as carbuncle skin infection.For dysuria, edema, or wet into diseases such as bet bladder, the puckery pains of dribbling urination.

Herba Pteridis Multifidae: light, micro-hardship is cool.Heat clearing away, dampness removing, removing heat from blood.Control bacillary dysentery, enteritis, fever caused by exogenous pathogens, urinary tract infection, leucorrhea, parotitis, furuncle, eczema." clearing away heat-damp and promoting diuresis, removing toxic substances dysentery relieving, cooling blood for hemostasis.For dysentery, gastroenteritis, hepatitis, urinary system infection, cat fever, laryngopharynx swelling and pain, leucorrhea, metrorrhagia, pesticide intoxication; Traumatic hemorrhage is controlled in external, burn and scald.

Herba Polygoni Avicularis: bitter, cold; Return urinary bladder channel.Diuresis, heat clearing away, parasite killing.Cure mainly and control pyretic stranguria, the difficulty in urination, jaundice, the erosion of the vulva, leucorrhea, ascarid, infantile malnutrition, hemorrhoid swell, eczema.

Herba Taraxaci: nature and flavor are sweet, micro-hardship, cold; Return spleen, stomach, kidney three warp.Apply all pyogenic infections from tumour or sore, the ruined tinea skin ulcer of scabies; Dispel the wind, disappear all sores, loose scrofula tuberculosis; Stop little having blood in stool, control the five types of stranguria difficulty in urination, sharp bladder.Heat-clearing and toxic substances removing, eliminating carbuncle eliminating stagnation.Can be used for upper respiratory tract infection, eye conjunctivitis, mumps, hyperglycemia, acute mastitis swells and ache, gastritis, dysentery, hepatitis, cholecystitis, acute appendicitis, urinary system infection, pelvic inflammatory disease, carbuncle furuncle furuncle, pharyngitis, controls acute mastitis, lymphadenitis, scrofula, furuncle carbuncle toxin, acute conjunctivitis, cold, fever, acute tonsillitis, acute bronchitis, urinary tract infection.

Folium Nelumbinis: bitter in the mouth is pungent micro-puckery, cool in nature; GUIXIN, liver, spleen channel.Cure mainly summer-heat excessive thirst, have a headache dizzy, edema, lack of appetite abdominal distention, dysentery, leucorrhea, proctoptosis, spit blood, epistaxis, spitting of blood, has blood in stool, metrorrhagia, post partum longtime lochia, traumatic blood stasis.

Plumula Nelumbinis: bitter, cold, nontoxic; GUIXIN, lung, kidney three warp.Clearing away heart-fire, suppressing the hyperactive liver fire, lung-fire, falls in purgation of spleen excess fire.Relieve summer heat relieving restlessness, promoting the production of body fluid to quench thirst, controls order redness.

Radix Ophiopogonis: sweet, the micro-hardship of property, is slightly cold; GUIXIN, lung, stomach warp.Have YIN nourishing and the production of body fluid promoting, effect that lung moistening clears away heart-fire, can be used for sore throat pharyngalgia, Tianjin wound is thirsty, and interior-heat is quenched one's thirst, vexed insomnia, dryness of the intestine constipation etc.

Fructus Forsythiae: bitter in the mouth, cold nature; GUIXIN, lung, small intestine meridian.Cure mainly calentura from the beginning of, anemopyretic cold, heating, vexed, the symptom such as laryngopharynx swelling and pain, acute nephritis, carbuncle sore tumefacting virus.

Radix Scrophulariae: sweet in the mouth, hardship, salty, cold nature, returns lung, stomach, kidney channel.There is clearing away heat and cooling blood, eliminating fire and detoxication, effect of YIN nourishing, cure mainly warm febrile disease and nutrient blood, fever of the body, excessive thirst, crimson tongue, the cards such as constipation, order puckery dim-sighted, throat larynx swells and ache, scrofula sucutaneous nodule, skin sore are not hindered in awake, Tianjin to send out speckle, hectic fever due to YIN-deficiency chronic cough, fidgets due to deficiency.

Radix Glycyrrhizae: nature and flavor are sweet, flat; Return spleen, stomach, lung meridian.Cold type of pain in main abdomen, controls infantile convulsion, except distention and fullness in the abdomen; Tonification the five internal organs; Make all poison of drug; Support kidney qi internal injury, make flaccidity; Housewife's human blood drop lumbago; Empty and frequent fever; Add and use it.Can QI invigorating invigorating middle warmer, relieving spasm to stop pain, nourishing the lung to arrest cough, eliminating fire and detoxication, coordinating the actions of various ingredients in a prescription.

Radix eriosematis chinensis: sweet; Flat.Return lung; Large intestine; Liver Channel.Removing heat from the lung and dissipating phlegm; Promoting the production of body fluid to quench thirst; Detumescence.Main cough due to lung-heat; Lung abscess; Heating excessive thirst; Dysentery; Hydrocele of testis, food stagnation does not disappear; Treating swelling and pain by traumatic injury.

Herba Diclipterae Chinensis: sweet in the mouth; Micro-hardship; Cold in nature.GUIXIN; Liver; Lung meridian.Heat clearing away; Removing heat from blood; Dampness removing; Removing toxic substances.Main cold, fever; Calentura sends out speckle; Tell epistaxis; Have blood in stool; Hematuria; Metrorrhagia; Cough due to lung-heat; Laryngopharynx swelling and pain; Liver-heat conjunctival congestion; Infantile convulsion; Dribbling urination; Leukorrhagia; Herpes zoster; Carbuncle furuncle furuncle; Serpentis lyssodexis.

Caulis et folium vitis thunbergii: sour, sweet, puckery is flat.Return liver; Stomach warp.Heat-clearing and toxic substances removing, expelling wind and removing dampness.For hepatitis, appendicitis, mastitis, lung abscess, multiple abscess, rheumatic arthritis; Sore swollen toxin is controlled in external, otitis media, snake bite and insect sting.

Radix Adenophorae Trachelioidis: sweet in the mouth; Cold in nature.Return lung; Spleen channel.Moistening the lung and resolving the phlegm; Heat-clearing and toxic substances removing.Main xeropulmonary cough; Laryngopharynx swelling and pain; Quench one's thirst; Furuncle carbuncle sore; Drug intoxication.

Radix Melothriae Heterophyllae: bitter in the mouth, cold in nature.Return lung, liver, spleen three warp.Removing heat-phlegm, dampness removing, mass dissipating and swelling eliminating.Control heat syndrome cough, dysentery, gonorrhea, urinary tract infection, alcoholic jaundice, rheumatic arthralgia, laryngalgia, conjunctival congestion, eczema, carbuncle.

Herba Cynanchi Thesioidis: sweet is flat.Tonifying the lung gas, resolving heat and reducing pathogenic fire, promoting the production of body fluid to quench thirst, anti-inflammatory analgetic.

Fructus Trichosanthis: sweet in the mouth; Micro-hardship; Cold in nature.Return lung; Stomach; Large intestine channel.Removing heat-phlegm; Wide born of the same parents' eliminating stagnation; Moisturize laxation.Main cough due to lung-heat; The thoracic obstruction; Quench one's thirst; Constipation; Carbuncle sore tumefacting virus.

Rhizoma Nephrolepis cordifoliae: sweet in the mouth band is puckery, property is put down, nontoxic.Promoting the production of body fluid to quench thirst, liver heat removing, hemostasis.Control dysentery of having loose bowels, dry tongue is thirsty, nebula on eyeball, and traumatic injury internal injury, knife injury is hemorrhage.

Concha Tridacna squamosa meat: sweet in the mouth; Salty; Cold in nature.Yin-nourishing and body fluid promoting; Heat-clearing and toxic substances removing.Main void wins interior-heat; Quench one's thirst; Flaccidity syndrome and arthralgia syndrome; Let out dysentery; Toxic swelling.

Rhizoma Dioscoreae Septemlobae: bitter; Flat.Return liver; Stomach; Urinary bladder channel.Wind-damp dispelling; Dampness removing is turbid.Main stranguria marked by chyluria; Nebulousurine; Leukorrhagia; Skin infection; Eczema; Rheumatic arthralgia.

Herba Ecliptae: sweet, sour, cold.Return kidney, Liver Channel.Nourishing the liver and kidney, cooling blood for hemostasis.For odontoseisis, early whitening of beard and hair, vertigo and tinnitus, soreness of the waist and knees, YinXuXueRe, haematemesis, epistaxis, hematuria, dysentery, metrostaxis, traumatic hemorrhage.

Semen Coicis: sweet, light, cool.Return spleen, stomach, lung meridian.Spleen invigorating eliminating dampness by diuresis, eliminating impediment antidiarrheal, row is dense in heat clearing away.For edema, beriberi, dysuria, arthralgia chiefly caused by damp pathogen contracture, diarrhea due to hypofunction of the spleen, lung abscess, intestinal carbuncle; Verruca plana.

Herba Moslae: pungent, tepor.Attach to the lung and stomach meridians.Relieving the exterior syndrome by diaphoresis, and middle dampness removing.For summer-heat damp cold, fever with aversion to cold, has a headache lossless, and stomachache is vomited and diarrhoea, dysuria.

Fructus Rubi: sweet, sour, temperature.Return kidney, urinary bladder channel.Kidney tonifying, controlling nocturnal emission with astringent drugs, reducing urination.For enuresis due to deficiency of the kidney, frequent micturition, impotence and premature ejaculation, emission and spermatorrhea.

Semen Hoveniae (Fructus Hoveniae): sweet in the mouth; Property is put down.GUIXIN; Spleen; Lung meridian.Alcoholic intoxication; To quench the thirst relieving restlessness; Preventing or arresting vomiting; Profit defecation.Main drunk; Excessive thirst; Vomiting; Difficulty in urination and defecation.

Below adopt embodiment to describe embodiments of the present invention in detail, to the present invention, how application technology means solve technical problem whereby, and the implementation procedure reaching technique effect can fully understand and implement according to this.

Embodiment 1 pharmaceutical composition 1

Embodiments provide a kind of pharmaceutical composition for preventing and treating chronic type swine fever epidemic disease, be made up of the raw medicinal material of following parts by weight: Mirabilitum crystallina 33 parts, Fructus Gardeniae 11 parts, 24 parts, the Radix Rehmanniae, Cacumen Platycladi 36 parts, Cortex Moutan 19 parts, Rhizoma Phragmitis 21 parts, Radix Trichosanthis 12 parts, Herba Lophatheri 35 parts, Herba Commelinae 29 parts, Herba Pteridis Multifidae 38 parts, Herba Polygoni Avicularis 14 parts, Herba Taraxaci 25 parts, 32 parts, Folium Nelumbinis, Plumula Nelumbinis 17 parts, Radix Ophiopogonis 15 parts, Fructus Forsythiae 16 parts, Radix Scrophulariae 39 parts, 32 parts, Radix Glycyrrhizae, Radix eriosematis chinensis 11 parts, Herba Diclipterae Chinensis 30 parts, Caulis et folium vitis thunbergii 40 parts, Radix Adenophorae Trachelioidis 5 parts, Radix Melothriae Heterophyllae 17 parts, Herba Cynanchi Thesioidis 24 parts, Fructus Trichosanthis 8 parts, Rhizoma Nephrolepis cordifoliae 17 parts, Concha Tridacna squamosa meat 35 parts of He Rhizoma Dioscoreae Septemlobae 16 parts.

Its preparation method comprises the following steps:

The first step, Mirabilitum crystallina, Fructus Gardeniae, the Radix Rehmanniae, Cacumen Platycladi, Cortex Moutan, Rhizoma Phragmitis, Radix Trichosanthis and Herba Lophatheri are mixed in described ratio, add the water extraction volatile oil relative to 5 times, mixture, by the volatile oil beta-cyclodextrin inclusion compound obtained, the mass ratio of volatile oil and beta-schardinger dextrin-is 1: 4, stirs 90 minutes, temperature 50 C, obtain volatile oil clathrate compound, for subsequent use; Another when aqueous extract being concentrated into 60 DEG C relative density be the clear paste of 1.17;

Second step, Herba Commelinae, Herba Pteridis Multifidae, Herba Polygoni Avicularis, Herba Taraxaci and Folium Nelumbinis are mixed in described ratio, add relative to mixture 6 times amount determining alcohol be 90% alcohol reflux 4 times, each 3 hours, filter respectively, each time filtrate merges, and reclaims ethanol, when being concentrated into 60 DEG C, relative density is the clear paste of 1.22, for subsequent use;

3rd step, will remain described raw medicinal material and mix in described ratio, add relative to mixture 10 times of water gagings, boil 8 hours, filter, and when being concentrated into 60 DEG C, relative density is the clear paste of 1.30;

4th step, mixed by the clear paste of the first step, second step, the 3rd step, 70 DEG C of reduced vacuum dryings, get dry extract powder;

5th step, dried cream powder described in volatile oil clathrate compound described in the first step and the 4th step is fully mixed, add the mixture relative to volatile oil clathrate compound described in 100 weight portions and dried cream powder subsequently, 6 weight portion carboxymethyl starch sodium 40 weight starch mix homogeneously, adding 200 weight portion determining alcohols is 90% alcohol granulation, dry, granulate, add the mixture relative to volatile oil clathrate compound described in 100 weight portions and dried cream powder subsequently, 4 weight portion magnesium stearate, then load in gelatine capsule.

Embodiment 2 pharmaceutical composition 2

Embodiments provide a kind of pharmaceutical composition for preventing and treating chronic type swine fever epidemic disease, be made up of the raw medicinal material of following parts by weight: Mirabilitum crystallina 33 parts, Fructus Gardeniae 11 parts, 24 parts, the Radix Rehmanniae, Cacumen Platycladi 36 parts, Cortex Moutan 19 parts, Rhizoma Phragmitis 21 parts, Radix Trichosanthis 12 parts, Herba Lophatheri 35 parts, Herba Commelinae 29 parts, Herba Pteridis Multifidae 38 parts, Herba Polygoni Avicularis 14 parts, Herba Taraxaci 25 parts, 32 parts, Folium Nelumbinis, Plumula Nelumbinis 17 parts, Radix Ophiopogonis 15 parts, Fructus Forsythiae 16 parts, Radix Scrophulariae 39 parts, 32 parts, Radix Glycyrrhizae, Radix eriosematis chinensis 11 parts, Herba Diclipterae Chinensis 30 parts, Caulis et folium vitis thunbergii 40 parts, Radix Adenophorae Trachelioidis 5 parts, Radix Melothriae Heterophyllae 17 parts, Herba Cynanchi Thesioidis 24 parts, Fructus Trichosanthis 8 parts, Rhizoma Nephrolepis cordifoliae 17 parts, Concha Tridacna squamosa meat 35 parts, Rhizoma Dioscoreae Septemlobae 16 parts, Herba Ecliptae 4 parts, Semen Coicis 17 parts, Herba Moslae 5 parts, Fructus Rubi 8 parts and Semen Hoveniae (Fructus Hoveniae) 10 parts.

Its preparation method is as follows:

Step one, all crude drug are dropped in proportion in blender stir, mixing, pulverize in rear feeding pulverizer, the Chinese medicine composition after pulverizing is loaded in multi-function extractor, add relative to its quality 6 times amount water, boil 3 hours, steam pressure is 0.7Mpa process, filter, obtain the first filtrate and filtering residue;

Step 2, the filtering residue of acquisition added again relative to its quality 6 times amount water, boil 3 hours, steam pressure is 0.8Mpa process, filter and obtain the second filtrate and filtering residue;

Step 3, the first filtrate and the second filtrate merged, concentrated removing moisture, obtains and gets dry extract, and adopts super-fine powder mill to pulverize, is crushed to less than 30 μm, obtain pharmaceutical composition.

The toxicity test of pharmaceutical composition:

1, trial drug

Choose Mirabilitum crystallina 33g, Fructus Gardeniae 11g, Radix Rehmanniae 24g, Cacumen Platycladi 36g, Cortex Moutan 19g, Rhizoma Phragmitis 21g, Radix Trichosanthis 12g, Herba Lophatheri 35g, Herba Commelinae 29g, Herba Pteridis Multifidae 38g, Herba Polygoni Avicularis 14g, Herba Taraxaci 25g, Folium Nelumbinis 32g, Plumula Nelumbinis 17g, Radix Ophiopogonis 15g, Fructus Forsythiae 16g, Radix Scrophulariae 39g, Radix Glycyrrhizae 32g, Radix eriosematis chinensis 11g, Herba Diclipterae Chinensis 30g, Caulis et folium vitis thunbergii 40g, Radix Adenophorae Trachelioidis 5g, Radix Melothriae Heterophyllae 17g, Herba Cynanchi Thesioidis 24g, Fructus Trichosanthis 8g, Rhizoma Nephrolepis cordifoliae 17g, Concha Tridacna squamosa meat 35g with Rhizoma Dioscoreae Septemlobae 16g mixes, be ground into powder; Added by acquisition powder in 1400g cold water and soak 25 hours, with slow fire boiling, decocting time is 1.5 hours, decoction liquor is filtered, obtains the first filtrate, collects filtering residue; Again add in 1400g cold water by filtering the filtering residue obtained, with slow fire boiling 3 times, each decoction 1 hour, filters and merges filtrate, obtaining the second filtrate; Merge twice filtrate, concentrating under reduced pressure remove portion water, obtain decoction, the crude drug concentration of decoction is 3g/ml.

2, test method: select SD rat, give the decoction of the Chinese medicine additive of the present invention of variable concentrations (18.0,6.0,2.0g crude drug/kg), every day gavage once, continuous 90 days, within after last administration 24 hours, respectively group is alive kills 1/2 animal (male and female half and half), and all the other 1/2 animals continue observations 2 weeks rear living and kill.Duration of test observes outward appearance, general behavior, food ration, the body weight change of animal, within after administration 90 days, within 2 weeks, carries out the index checking such as hematology's (RBC, HB, reticulocyte, PLT, CT, WBC and classification) and blood biochemical (AST, ALT, ALP, Glu, BUN, Crca, TP, T.BIL, ALB, CHOL), urine biochemistry, organ coefficient, histopathology with drug withdrawal.Result of the test shows: Chinese medicine additive of the present invention is good at high, medium and low dosage treated animal general state, and outward appearance sign, behavioral activity, food-intake and body weight increase change all without exception; Three dosage groups and matched group hematological examination, blood biochemical analysis, urine biochemical analysis all in normal range, without significant difference between group; Each group of main organs histopathological examination shows no obvious abnormalities.These parameters drug withdrawal also had no change after 2 weeks.This test dosage is respectively 180,60,20 times of clinical medicine dose, rat is had no significant effect in high, medium and low three dosage (18.0,6.0,2.0g crude drug/kg) administration in continuous 90 days according to result of the test Chinese medicine preparation of the present invention, without clear and definite toxicity target organ and sensitive indicator, convalescent period observation also has no retardance toxic reaction, points out the dose safety of Chinese medicine preparation clinical practice of the present invention higher.

The toxicity test of pharmaceutical composition:

Get Mirabilitum crystallina 33g, Fructus Gardeniae 11g, Radix Rehmanniae 24g, Cacumen Platycladi 36g, Cortex Moutan 19g, Rhizoma Phragmitis 21g, Radix Trichosanthis 12g, Herba Lophatheri 35g, Herba Commelinae 29g, Herba Pteridis Multifidae 38g, Herba Polygoni Avicularis 14g, Herba Taraxaci 25g, Folium Nelumbinis 32g, Plumula Nelumbinis 17g, Radix Ophiopogonis 15g, Fructus Forsythiae 16g, Radix Scrophulariae 39g, Radix Glycyrrhizae 32g, Radix eriosematis chinensis 11g, Herba Diclipterae Chinensis 30g, Caulis et folium vitis thunbergii 40g, Radix Adenophorae Trachelioidis 5g, Radix Melothriae Heterophyllae 17g, Herba Cynanchi Thesioidis 24g, Fructus Trichosanthis 8g, Rhizoma Nephrolepis cordifoliae 17g, Concha Tridacna squamosa meat 35g, Rhizoma Dioscoreae Septemlobae 16g, Herba Ecliptae 4g, Semen Coicis 17g, Herba Moslae 5g, Fructus Rubi 8g and Semen Hoveniae (Fructus Hoveniae) 10g mix, and is ground into powder; Added by acquisition powder in 1400g cold water and soak 25 hours, with slow fire boiling, decocting time is 1.5 hours, decoction liquor is filtered, obtains the first filtrate, collects filtering residue; Again add in 1400g cold water by filtering the filtering residue obtained, with slow fire boiling 3 times, each decoction 1 hour, filters and merges filtrate, obtaining the second filtrate; Merge twice filtrate, concentrating under reduced pressure remove portion water, obtain decoction, the crude drug concentration of decoction is 3g/ml.

2, test method: select SD rat, give the decoction of the Chinese medicine additive of the present invention of variable concentrations (18.0,6.0,2.0g crude drug/kg), every day gavage once, continuous 90 days, within after last administration 24 hours, respectively group is alive kills 1/2 animal (male and female half and half), and all the other 1/2 animals continue observations 2 weeks rear living and kill.Duration of test observes outward appearance, general behavior, food ration, the body weight change of animal, within after administration 90 days, within 2 weeks, carries out the index checking such as hematology's (RBC, HB, reticulocyte, PLT, CT, WBC and classification) and blood biochemical (AST, ALT, ALP, Glu, BUN, Crca, TP, T.BIL, ALB, CHOL), urine biochemistry, organ coefficient, histopathology with drug withdrawal.Result of the test shows: Chinese medicine additive of the present invention is good at high, medium and low dosage treated animal general state, and outward appearance sign, behavioral activity, food-intake and body weight increase change all without exception; Three dosage groups and matched group hematological examination, blood biochemical analysis, urine biochemical analysis all in normal range, without significant difference between group; Each group of main organs histopathological examination shows no obvious abnormalities.These parameters drug withdrawal also had no change after 2 weeks.This test dosage is respectively 180,60,20 times of clinical medicine dose, rat is had no significant effect in high, medium and low three dosage (18.0,6.0,2.0g crude drug/kg) administration in continuous 90 days according to result of the test Chinese medicine preparation of the present invention, without clear and definite toxicity target organ and sensitive indicator, convalescent period observation also has no retardance toxic reaction, points out the dose safety of Chinese medicine preparation clinical practice of the present invention higher.

Contrast test

Pig farm, Hebei Handan City, since in April, 2010, in the piglet group of wean (40 ~ 60 age in days), the mortality rate that lasting appearance is higher.Low during height when the cardinal symptom of sick pig is body temperature, diarrhoea (feces is yellowish), becomes thin, inappetence, and secret and diarrhoea is normal alternately to be occurred, sick pig skeletonize, anemia, hind limb weakness during walking.There is bluish violet or necrosis in some sick pig have sharp ears and extremity.Cut open inspection in the pathological changes of septicemia, intestinal has hyperemia in various degree, hemorrhage, at large intestine and ileal valve position, obvious ulcer occurs.Lymphadenectasis, hyperemia, hemorrhage and edema.Once in the body of some sick pigs, be separated to the antibacterials such as escherichia coli, Salmonella and streptococcus, but use multiple antibacterials and take corresponding prophylactico-therapeutic measures, produced little effect.Analyse and be diagnosed as chronic type swine fever.

Choose the ill piglet 126 that 45 ages in days are made a definite diagnosis, 126 ill piglets are divided into three groups at random, be respectively embodiment group 1, embodiment group 2 and matched group, the ill piglet of three groups all without significant difference, has comparability in body weight, the state of an illness etc.Embodiment group 1, embodiment group 2 and matched group use the powder of the capsule of the embodiment of the present invention 1, embodiment 2 and conventional medicament to treat respectively; Wherein, the using method of capsule and powder is mixed in conventional feed by medicament according to the part by weight of 3:100, feeds continuously 7 days; Conventional medicament is subcutaneous injection hyper-immune serum 0.4 milliliter/KG, once-a-day, and astragalin injection intramuscular injection 3ml/ time, 2 times on the one; Simultaneously in feedstuff, add oxytetracycline according to 2:1000 to mix conventional feed and feed, conventional feed of feeding.Experimental period is 7 days, takes each group of ill pig weight respectively before and after experiment, and period records recovery from illness situation and the death toll of ill piglet, and calculate average daily gain and cure rate, result as shown in Table 1 and Table 2.Wherein, cure: spirit is normal, drinking-water of searching for food is normal, and feces is normal, bouncing, and it is normal that the histoorgans such as inspection liver, intestinal, fabricius bursa, pancreas are cutd open in sampling; Invalid: clinical symptoms is not improved and even increased the weight of or death.

Table 1 each experimental group piglet average weight synopsis

Data from table 1: compared with matched group, use the ill piglet of Drug therapy of the present invention, during to piglet 52 age in days, the piglet that its average weight is treated than traditional therapy exceeds 0.9kg and 1.5kg respectively, and average daily gain exceeds 0.13kg/d and 0.20kg/d respectively.

The therapeutic effect synopsis of each experimental group piglet of table 2

Data from table 2: compared with conventional medicament, the medicine of the embodiment of the present invention 1 and 2 has advantage that is evident in efficacy, cheap, that have no side effect in treatment chronic type swine fever.

Known by contrast test: compared with conventional medicament, the pharmaceutical composition of the embodiment of the present invention 1 and 2 has toxic and side effects advantage little, evident in efficacy in treatment chronic type swine fever, but also can be good at the normal growth ensureing piglet.

The piglet of 150 40-55 ages in days of Hebei in January, 2013 Handan self-employed pig raiser raising, nearly becoming thin appears in 27 piglets, anemia, and asthenia universalis, often lies prostrate, and gait is slowly unable, inappetence, and constipation and diarrhoea are alternately.Cut open inspection pig and show as splanchnocoel hydrops, visceral pericardium is hemorrhage, and liver turns yellow, and there is volume petechia on surface, spleen edge infarction, and lymph nodes of body as a whole edema, hyperemia, periphery are hemorrhage.Kidney is khaki, and there is the petechia of needle point size on surface, and medullary substance, renal pelvis portion are hemorrhage, and mucous membrane of urinary bladder has the petechia of being dispersed in property.Mesentery edema, colorectal mucosa is hemorrhage, hemorrhage of tonsil, and larynx myxedema has hemorrhage speckle.Pick up from spleen and the lymph node of the routine sick pig in pig farm 5, get pathological material of disease suspension point sample on nitrocellulose filter, natural drying; Use 10% skim milk, close 30 minutes for 37 DEG C; With PBS rinsing 3 times; The anti-hog cholera antibody IgG of enzyme-added mark, 37 DEG C are reacted 2 hours; The same rinsing; Add DAB colour developing lucifuge and react 15 minutes; Tap water rinse, cessation reaction; Occur that rufous speckle is judged to the positive.

Be diagnosed as chronic type swine fever.Place is raised immediately by not occurring that the piglet of symptom isolates.Choose 13 ill piglets wherein, start to feed and be added with the feedstuff of the embodiment of the present invention 2 powder, powder is mixed in conventional feed according to 3:100 weight ratio, feed 7 days, within 3rd day, ill piglet symptom is obviously improved, and within the 5th day, 13 piglets are all fully recovered, after continuing to feed 4 days, compared with sodium selenite, average weight is substantially identical.

The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.

Claims (10)

1. for preventing and treating the pharmaceutical composition of chronic type swine fever epidemic disease, it is characterized in that, described pharmaceutical composition comprises following raw medicinal material: Mirabilitum crystallina, Fructus Gardeniae, the Radix Rehmanniae, Cacumen Platycladi, Cortex Moutan, Rhizoma Phragmitis, Radix Trichosanthis, Herba Lophatheri, Herba Commelinae, Herba Pteridis Multifidae, Herba Polygoni Avicularis, Herba Taraxaci, Folium Nelumbinis, Plumula Nelumbinis, Radix Ophiopogonis, Fructus Forsythiae, Radix Scrophulariae, Radix Glycyrrhizae, Radix eriosematis chinensis, Herba Diclipterae Chinensis, Caulis et folium vitis thunbergii, Radix Adenophorae Trachelioidis, Radix Melothriae Heterophyllae, Herba Cynanchi Thesioidis, Fructus Trichosanthis, Rhizoma Nephrolepis cordifoliae, Concha Tridacna squamosa meat are with Rhizoma Dioscoreae Septemlobae.

2. the pharmaceutical composition for preventing and treating chronic type swine fever epidemic disease according to claim 1, it is characterized in that, in described pharmaceutical composition, the parts by weight of each raw medicinal material are: Mirabilitum crystallina 23-36 part, Fructus Gardeniae 7-15 part, Radix Rehmanniae 17-35 part, Cacumen Platycladi 25-40 part, Cortex Moutan 9-25 part, Rhizoma Phragmitis 13-27 part, Radix Trichosanthis 10-28 part, Herba Lophatheri 25-38 part, Herba Commelinae 28-40 part, Herba Pteridis Multifidae 26-39 part, Herba Polygoni Avicularis 12-32 part, Herba Taraxaci 12-37 part, Folium Nelumbinis 20-35 part, Plumula Nelumbinis 15-28 part, Radix Ophiopogonis 15-30 part, Fructus Forsythiae 12-25 part, Radix Scrophulariae 25-40 part, Radix Glycyrrhizae 15-32 part, Radix eriosematis chinensis 11-23 part, Herba Diclipterae Chinensis 17-30 part, Caulis et folium vitis thunbergii 26-42 part, Radix Adenophorae Trachelioidis 3-10 part, Radix Melothriae Heterophyllae 15-29 part, Herba Cynanchi Thesioidis 20-37 part, Fructus Trichosanthis 7-23 part, Rhizoma Nephrolepis cordifoliae 11-29 part, Concha Tridacna squamosa meat 20-38 part is with Rhizoma Dioscoreae Septemlobae 14-29 part.

3. the pharmaceutical composition for preventing and treating chronic type swine fever epidemic disease according to claim 1 and 2, it is characterized in that, in described pharmaceutical composition, the parts by weight of each raw medicinal material are: Mirabilitum crystallina 33 parts, Fructus Gardeniae 11 parts, 24 parts, the Radix Rehmanniae, Cacumen Platycladi 36 parts, Cortex Moutan 19 parts, Rhizoma Phragmitis 21 parts, Radix Trichosanthis 12 parts, Herba Lophatheri 35 parts, Herba Commelinae 29 parts, Herba Pteridis Multifidae 38 parts, Herba Polygoni Avicularis 14 parts, Herba Taraxaci 25 parts, 32 parts, Folium Nelumbinis, Plumula Nelumbinis 17 parts, Radix Ophiopogonis 15 parts, Fructus Forsythiae 16 parts, Radix Scrophulariae 39 parts, 32 parts, Radix Glycyrrhizae, Radix eriosematis chinensis 11 parts, Herba Diclipterae Chinensis 30 parts, Caulis et folium vitis thunbergii 40 parts, Radix Adenophorae Trachelioidis 5 parts, Radix Melothriae Heterophyllae 17 parts, Herba Cynanchi Thesioidis 24 parts, Fructus Trichosanthis 8 parts, Rhizoma Nephrolepis cordifoliae 17 parts, Concha Tridacna squamosa meat 35 parts of He Rhizoma Dioscoreae Septemlobae 16 parts.

4. the pharmaceutical composition for preventing and treating chronic type swine fever epidemic disease according to any one of claim 1-3, it is characterized in that, described pharmaceutical composition also comprises Herba Ecliptae, Semen Coicis, Herba Moslae, Fructus Rubi and Semen Hoveniae (Fructus Hoveniae).

5. the pharmaceutical composition for preventing and treating chronic type swine fever epidemic disease according to any one of claim 1-4, it is characterized in that, in described pharmaceutical composition, the parts by weight of each raw medicinal material are: Mirabilitum crystallina 23-36 part, Fructus Gardeniae 7-15 part, Radix Rehmanniae 17-35 part, Cacumen Platycladi 25-40 part, Cortex Moutan 9-25 part, Rhizoma Phragmitis 13-27 part, Radix Trichosanthis 10-28 part, Herba Lophatheri 25-38 part, Herba Commelinae 28-40 part, Herba Pteridis Multifidae 26-39 part, Herba Polygoni Avicularis 12-32 part, Herba Taraxaci 12-37 part, Folium Nelumbinis 20-35 part, Plumula Nelumbinis 15-28 part, Radix Ophiopogonis 15-30 part, Fructus Forsythiae 12-25 part, Radix Scrophulariae 25-40 part, Radix Glycyrrhizae 15-32 part, Radix eriosematis chinensis 11-23 part, Herba Diclipterae Chinensis 17-30 part, Caulis et folium vitis thunbergii 26-42 part, Radix Adenophorae Trachelioidis 3-10 part, Radix Melothriae Heterophyllae 15-29 part, Herba Cynanchi Thesioidis 20-37 part, Fructus Trichosanthis 7-23 part, Rhizoma Nephrolepis cordifoliae 11-29 part, Concha Tridacna squamosa meat 20-38 part, Rhizoma Dioscoreae Septemlobae 14-29 part, Herba Ecliptae 3-9 part, Semen Coicis 12-19 part, Herba Moslae 4-8 part, Fructus Rubi 5-11 part and Semen Hoveniae (Fructus Hoveniae) 7-12 part.

6. the pharmaceutical composition for preventing and treating chronic type swine fever epidemic disease according to any one of claim 1-5, it is characterized in that, in described pharmaceutical composition, the parts by weight of each raw medicinal material are: Mirabilitum crystallina 33 parts, Fructus Gardeniae 11 parts, 24 parts, the Radix Rehmanniae, Cacumen Platycladi 36 parts, Cortex Moutan 19 parts, Rhizoma Phragmitis 21 parts, Radix Trichosanthis 12 parts, Herba Lophatheri 35 parts, Herba Commelinae 29 parts, Herba Pteridis Multifidae 38 parts, Herba Polygoni Avicularis 14 parts, Herba Taraxaci 25 parts, 32 parts, Folium Nelumbinis, Plumula Nelumbinis 17 parts, Radix Ophiopogonis 15 parts, Fructus Forsythiae 16 parts, Radix Scrophulariae 39 parts, 32 parts, Radix Glycyrrhizae, Radix eriosematis chinensis 11 parts, Herba Diclipterae Chinensis 30 parts, Caulis et folium vitis thunbergii 40 parts, Radix Adenophorae Trachelioidis 5 parts, Radix Melothriae Heterophyllae 17 parts, Herba Cynanchi Thesioidis 24 parts, Fructus Trichosanthis 8 parts, Rhizoma Nephrolepis cordifoliae 17 parts, Concha Tridacna squamosa meat 35 parts, Rhizoma Dioscoreae Septemlobae 16 parts, Herba Ecliptae 4 parts, Semen Coicis 17 parts, Herba Moslae 5 parts, Fructus Rubi 8 parts and Semen Hoveniae (Fructus Hoveniae) 10 parts.

7. the pharmaceutical composition for preventing and treating chronic type swine fever epidemic disease according to any one of claim 1-6, is characterized in that, the dosage form of described pharmaceutical composition is capsule, and its preparation method comprises the following steps:

The first step, Mirabilitum crystallina, Fructus Gardeniae, the Radix Rehmanniae, Cacumen Platycladi, Cortex Moutan, Rhizoma Phragmitis, Radix Trichosanthis and Herba Lophatheri are mixed in described ratio, add the water extraction volatile oil relative to 4 ~ 6 times, mixture, by the volatile oil beta-cyclodextrin inclusion compound obtained, the mass ratio of volatile oil and beta-schardinger dextrin-is 1: 3 ~ 6, stirs 60 minutes ~ 90 minutes, temperature 30 DEG C ~ 50 DEG C, obtain volatile oil clathrate compound, for subsequent use; Another when aqueous extract being concentrated into 60 DEG C relative density be the clear paste of 1.15 ~ 1.17;

Second step, Herba Commelinae, Herba Pteridis Multifidae, Herba Polygoni Avicularis, Herba Taraxaci and Folium Nelumbinis are mixed in described ratio, add relative to mixture 5 ~ 8 times amount determining alcohol be 75% ~ 90% alcohol reflux 3 ~ 5 times, each 2 ~ 3 hours, filter respectively, each time filtrate merges, and reclaims ethanol, when being concentrated into 60 DEG C, relative density is the clear paste of 1.20 ~ 1.22, for subsequent use;

3rd step, will remain described raw medicinal material and mix in described ratio, add relative to mixture 8 ~ 10 times of water gagings, boil 6 ~ 8 hours, filter, and when being concentrated into 60 DEG C, relative density is the clear paste of 1.28 ~ 1.30;

4th step, mixed by the clear paste of the first step, second step, the 3rd step, 60 DEG C ~ 70 DEG C reduced vacuum dryings, get dry extract powder;

5th step, dried cream powder described in volatile oil clathrate compound described in the first step and the 4th step is fully mixed, add the mixture relative to volatile oil clathrate compound described in 100 weight portions and dried cream powder subsequently, 5 ~ 7 weight portion carboxymethyl starch sodium, 5 ~ 50 weight starch mix homogeneously, adding 150 ~ 200 weight portion determining alcohols is 80% ~ 90% alcohol granulation, dry, granulate, add the mixture relative to volatile oil clathrate compound described in 100 weight portions and dried cream powder subsequently, 3 ~ 5 weight portion magnesium stearate, then load in gelatine capsule.

8. the pharmaceutical composition for preventing and treating chronic type swine fever epidemic disease according to any one of claim 1-6, is characterized in that, the dosage form of described pharmaceutical composition is powder, and its preparation method comprises the following steps:

Step one, all crude drug are dropped in proportion in blender stir, mixing, pulverize in rear feeding pulverizer, the Chinese medicine composition after pulverizing is loaded in multi-function extractor, add relative to its quality 5-7 times amount water, boil 2-4 hour, steam pressure is 0.6-1Mpa process, filter, obtain the first filtrate and filtering residue;

Step 2, the filtering residue of acquisition added again relative to its quality 5-7 times amount water, boil 2-4 hour, steam pressure is 0.6Mpa-1Mpa process, filter and obtain the second filtrate and filtering residue;

Step 3, the first filtrate and the second filtrate merged, concentrated removing moisture, obtains and gets dry extract, and adopts super-fine powder mill to pulverize, is crushed to less than 30 μm, obtain pharmaceutical composition powder.

9. a preparation method for the pharmaceutical composition for preventing and treating chronic type swine fever epidemic disease as described in any one of claim 1-6, it is characterized in that, the dosage form of described pharmaceutical composition is capsule, and its preparation method comprises the following steps:

The first step, Mirabilitum crystallina, Fructus Gardeniae, the Radix Rehmanniae, Cacumen Platycladi, Cortex Moutan, Rhizoma Phragmitis, Radix Trichosanthis and Herba Lophatheri are mixed in described ratio, add the water extraction volatile oil relative to 4 ~ 6 times, mixture, by the volatile oil beta-cyclodextrin inclusion compound obtained, the mass ratio of volatile oil and beta-schardinger dextrin-is 1: 3 ~ 6, stirs 60 minutes ~ 90 minutes, temperature 30 DEG C ~ 50 DEG C, obtain volatile oil clathrate compound, for subsequent use; Another when aqueous extract being concentrated into 60 DEG C relative density be the clear paste of 1.15 ~ 1.17;

Second step, Herba Commelinae, Herba Pteridis Multifidae, Herba Polygoni Avicularis, Herba Taraxaci and Folium Nelumbinis are mixed in described ratio, add relative to mixture 5 ~ 8 times amount determining alcohol be 75% ~ 90% alcohol reflux 3 ~ 5 times, each 2 ~ 3 hours, filter respectively, each time filtrate merges, and reclaims ethanol, when being concentrated into 60 DEG C, relative density is the clear paste of 1.20 ~ 1.22, for subsequent use;

3rd step, will remain described raw medicinal material and mix in described ratio, add relative to mixture 8 ~ 10 times of water gagings, boil 6 ~ 8 hours, filter, and when being concentrated into 60 DEG C, relative density is the clear paste of 1.28 ~ 1.30;

4th step, mixed by the clear paste of the first step, second step, the 3rd step, 60 DEG C ~ 70 DEG C reduced vacuum dryings, get dry extract powder;

5th step, dried cream powder described in volatile oil clathrate compound described in the first step and the 4th step is fully mixed, add the mixture relative to volatile oil clathrate compound described in 100 weight portions and dried cream powder subsequently, 5 ~ 7 weight portion carboxymethyl starch sodium, 5 ~ 50 weight starch mix homogeneously, adding 150 ~ 200 weight portion determining alcohols is 80% ~ 90% alcohol granulation, dry, granulate, add the mixture relative to volatile oil clathrate compound described in 100 weight portions and dried cream powder subsequently, 3 ~ 5 weight portion magnesium stearate, then load in gelatine capsule.

10. a preparation method for the pharmaceutical composition for preventing and treating chronic type swine fever epidemic disease as described in any one of claim 1-6, it is characterized in that, the dosage form of described pharmaceutical composition is powder, and its preparation method comprises the following steps:

Step one, all crude drug are dropped in proportion in blender stir, mixing, pulverize in rear feeding pulverizer, the Chinese medicine composition after pulverizing is loaded in multi-function extractor, add relative to its quality 5-7 times amount water, boil 2-4 hour, steam pressure is 0.6-1Mpa process, filter, obtain the first filtrate and filtering residue;

Step 2, the filtering residue of acquisition added again relative to its quality 5-7 times amount water, boil 2-4 hour, steam pressure is 0.6Mpa-1Mpa process, filter and obtain the second filtrate and filtering residue;

Step 3, the first filtrate and the second filtrate merged, concentrated removing moisture, obtains and gets dry extract, and adopts super-fine powder mill to pulverize, is crushed to less than 30 μm, obtain pharmaceutical composition powder.