CN103142943B - Traditional Chinese medicine composite with immune function and used for treating mastitis of cows - Google Patents
Traditional Chinese medicine composite with immune function and used for treating mastitis of cows Download PDFInfo
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Abstract
The invention provides a traditional Chinese medicine composite with an immune function and used for treating mastitis of cows. The traditional Chinese medicine composite contains the following crude medicines: sweetgum fruits, liquorice, rhizoma polygonati, herba violae, Chinese pulsatilla roots, rhizoma panacis majoris, eucommia bark, folium isatidis, myrrh, cortex moutan, folium eriobotryae, coptis roots, multiflower knotweed vines, white paeony roots, turmeric, radix astragali, rhizoma atractylodis macrocephalae, fructus choerospondiatis, schisandra chinensis and radix glehniae. The traditional Chinese medicine composite is capable of improving the immunity of the cows simultaneously when clearing heat, detoxifying, and anti-bacterially decreasing inflammation, thereby improving the anti-stress and the anti-disease capability of the cows; and the traditional Chinese medicine composite has the advantages of good treatment effect, reoccurrence prevention, no medicine resistance, no residue, and no toxic and side effects in treating mastitis of the cows.
Description
Technical field
The present invention relates to Chinese medicine immunological technique field, relate in particular to a kind of Chinese medicine composition that is used for the treatment of bovine mastitis with immunologic function.
Background technology
The mammary gland of milch cow is a complex biological reactor in body; in mammary gland inside; numerous protection factors comprises together with cytokine, antibody, hormone, enzyme and neurotransmitter etc. and built many complicated signal paths, and the dynamic equilibrium between these factors is the primary condition that maintains Breast health.In the time that cow mammary gland is inflamed, Abwehrkraft des Koepers declines, and the expression of the cells in vivo factor and receptor thereof will occur extremely.
Bovine mastitis (mastitis) refers in the mammary gland tissue of milch cow the inflammation of certain type has occurred, and with milk physics, chemistry and microbiology change in various degree, it is that ruminant sickness rate is the highest, one of modal disease, is the mammitis reaction causing due to organism metabolism and physiological status change, injury of breast and infection or 20th-century disease pathogenic microorganism etc.It is normally caused by combined factors effects such as cause pathogeny imcrobe infection, feeding and management, environment, milch cow self and heredity.The generation of mastitis not only makes the time lengthening of rutting and tire spacing elongate, and has reduced the fertility performance of milch cow, causes milk yield to decline, and even breast necrosis, loses milking capacity.The economic loss causing because of bovine mastitis every year, approximately 2,000,000,000 dollars, the U.S., approximately 300,000,000 pounds, Britain, China is every year also in several hundred million RMB, but also seriously affected the quality of milk product, and indirectly jeopardizes human health.
At present, mainly antibiotic to the medicine of bovine mastitis both at home and abroad, antibiotic is the essential drugs of current mastitis treatment, but the therapeutic effect of this type of medicine is normal and unsatisfactory, and the drug residue problem that it causes is more and more subject to numerous blames.In recent years, animal products quality security problem has become a principal contradiction of China's animal husbandry development.A large amount of use antibiotic of cattle farm will have a negative impact to dairy products' quality safety.China's dairy mastitis sickness rate is higher, antibiotic uses frequent, the drug residue phenomenon that exceeds standard is more serious, grievous injury the dairy competitiveness of product in market, how under the prerequisite that ensures milk cow health, to reduce antibiotic use is the important topic that dairy faces.
What adopt that antibiotic therapy selects is simple antibacterial thinking, has only paid attention to the killing of microorganism, and has not paid attention to as contradiction in mastitis process on the other hand the problem of the immunity degradation that inflammatory reaction of milch cow itself and inflammation cause.Pathology think, inflammation is a kind of defense reaction of body to pessimal stimulation, is a pathological process.Certainly, infected by microbes is often the main stimulating factor causing inflammation, but inflammation does not merely equal to infect.Mastitis are inflammation of mammary gland, and the reaction of infected by microbes and mammary gland tissue is two main aspects of its contradiction.As can be seen here, current mastitis are studied many problems of paying attention to infected by microbes, and have ignored another main aspect of contradiction.The inflammatory reaction of appropriateness is conducive to the removing of infector, but inflammatory reaction intensity can cause heavier microcirculatory injury and obstacle when excessive, and the aggravation of Shi Yan district local edema, mass exchange are obstructed, harmful metabolite is difficult to discharge.Now, even if infector is removed completely or substantially, local a large amount of harmful products and accumulating of inflammatory mediator still cause mammary gland tissue still to maintain inflammatory conditions.Meanwhile, tissue edema also hinders medicine and enters scorching district, reduces the result of use of various medicines.Can judge their early stage, taking infected by microbes as principal contradiction, and along with advancing of disease, in mammary gland, inflammatory reaction reinforcement, infection are controlled, and principal contradiction changes the hypoimmunity that inflammation causes very soon into, and premunition weakens.Cure clinically people, same, the past thinks that infective inflammation is that antibacterial or virus cause always, as long as eliminate antibacterial or virus, inflammation will disappear.Since the 90's, in various infection and noninfectious disease, the too drastic and hypoimmunity that causes of body inflammatory reaction, premunition weakens oneself and obtains more attention.
In sum, mammary gland immune system has material impact to the process of clinical mastitis.Also have fundamental significance and improve immune system Dui Yan district tissue repair, the reparation of tissue is directly connected to again the maintenance of milking capacity after being ill.So, to the obvious case of those clinical symptoms, should change original simple antibacterial thinking, the reaction itself that controls inflammation, improve immune system as one of main points for the treatment of.
Chinese medicine belongs to natural drug, has antibacterial, antiinflammatory, is difficult for making pathogenic bacterium to produce the effect of drug resistance and immunostimulant, has the feature of safety, efficient, low toxicity, low-residual, can overcome the drawback that a large amount of use antibiotic bring.
Chinese herbal medicine has enhancing human body immunity function, improves animal anti-stress, anti-disease ability, improves the multiple efficacies such as breeding performonce fo animals, and have no drug resistance, noresidue, toxic and side effects be little, demonstrates unique advantage aspect livestock and poultry cultivation.
Chinese medicine has the effect for the treatment of both the principal and secondary aspects of a disease, and it can directly suppress or kill the pathogen of mastitis, or suppresses or kill pathogen by improving the immunity of body, aspect treatment bovine mastitis, has huge potentiality.Therefore, carrying out the not still requirement of sustainable animal husbandry development of herbal control bovine mastitis, is also the requirement that people improve the quality of living and pollution-free food is consumed.
The present invention is exactly on the basis of Chinese veterinarian's theory, a kind of Chinese medicine composition that is used for the treatment of bovine mastitis with immunologic function is provided, it can improve the immunity of milch cow in anti-inflammation, heat-clearing and toxic substances removing, thereby realizes the fundamentally treatment to bovine mastitis.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of Chinese medicine composition that is used for the treatment of bovine mastitis with immunologic function, it is at heat-clearing and toxic substances removing, when anti-inflammation, can improve the immunocompetence of milch cow, thereby improve the anti-stress of milch cow, anti-disease ability, aspect treatment cow mammary gland, have good effect, anti-recurrence, has no drug resistance, noresidue, the advantage having no side effect.
In order to solve the problems of the technologies described above, the invention provides a kind of Chinese medicine composition that is used for the treatment of bovine mastitis with immunologic function, its crude drug comprises Fructus Liquidambaris, Radix Glycyrrhizae, Rhizoma Polygonati, Herba Violae, the Radix Pulsatillae, Rhizoma Panacis Majoris, the Cortex Eucommiae, Folium Isatidis, Myrrha, Cortex Moutan, Folium Eriobotryae, Rhizoma Coptidis, Caulis Polygoni Multiflori, the Radix Paeoniae Alba, Rhizoma Curcumae Longae, the Radix Astragali, the Rhizoma Atractylodis Macrocephalae, Fructus Choerospondiatis, Fructus Schisandrae Chinensis and Radix Glehniae.
Wherein, in described Chinese medicine composition, the weight portion of each crude drug is respectively: Fructus Liquidambaris 15 weight portion ~ 25 weight portions, Radix Glycyrrhizae 30 weight portion ~ 40 weight portions, Rhizoma Polygonati 60 weight portion ~ 70 weight portions, Herba Violae 20 weight portion ~ 30 weight portions, the Radix Pulsatillae 35 weight portion ~ 45 weight portions, Rhizoma Panacis Majoris 55 weight portion ~ 65 weight portions, the Cortex Eucommiae 40 weight portion ~ 50 weight portions, Folium Isatidis 55 weight portion ~ 65 weight portions, Myrrha 20 weight portion ~ 30 weight portions, Cortex Moutan 35 weight portion ~ 45 weight portions, Folium Eriobotryae 15 weight portion ~ 25 weight portions, Rhizoma Coptidis 50 weight portion ~ 60 weight portions, Caulis Polygoni Multiflori 35 weight portion ~ 45 weight portions, the Radix Paeoniae Alba 30 weight portion ~ 40 weight portions, Rhizoma Curcumae Longae 30 weight portion ~ 40 weight portions, the Radix Astragali 80 weight portion ~ 90 weight portions, the Rhizoma Atractylodis Macrocephalae 50 weight portion ~ 60 weight portions, Fructus Choerospondiatis 20 weight portion ~ 30 weight portions, Fructus Schisandrae Chinensis 40 weight portion ~ 50 weight portions and Radix Glehniae 30 weight portion ~ 40 weight portions.
Wherein, described Chinese medicine composition dosage form is decoction, powder and injection.
The present invention also provides above-mentioned Chinese medicine composition decoction, and the crude drug in described decoction comprises Fructus Liquidambaris, Radix Glycyrrhizae, Rhizoma Polygonati, Herba Violae, the Radix Pulsatillae, Rhizoma Panacis Majoris, the Cortex Eucommiae, Folium Isatidis, Myrrha, Cortex Moutan, Folium Eriobotryae, Rhizoma Coptidis, Caulis Polygoni Multiflori, the Radix Paeoniae Alba, Rhizoma Curcumae Longae, the Radix Astragali, the Rhizoma Atractylodis Macrocephalae, Fructus Choerospondiatis, Fructus Schisandrae Chinensis and Radix Glehniae.
The present invention also provides above-mentioned Chinese medicine composition composition powders, and the crude drug in described powder comprises Fructus Liquidambaris, Radix Glycyrrhizae, Rhizoma Polygonati, Herba Violae, the Radix Pulsatillae, Rhizoma Panacis Majoris, the Cortex Eucommiae, Folium Isatidis, Myrrha, Cortex Moutan, Folium Eriobotryae, Rhizoma Coptidis, Caulis Polygoni Multiflori, the Radix Paeoniae Alba, Rhizoma Curcumae Longae, the Radix Astragali, the Rhizoma Atractylodis Macrocephalae, Fructus Choerospondiatis, Fructus Schisandrae Chinensis and Radix Glehniae.
The present invention also provides above-mentioned Chinese medicine composition composition injection, and the crude drug in described injection comprises Fructus Liquidambaris, Radix Glycyrrhizae, Rhizoma Polygonati, Herba Violae, the Radix Pulsatillae, Rhizoma Panacis Majoris, the Cortex Eucommiae, Folium Isatidis, Myrrha, Cortex Moutan, Folium Eriobotryae, Rhizoma Coptidis, Caulis Polygoni Multiflori, the Radix Paeoniae Alba, Rhizoma Curcumae Longae, the Radix Astragali, the Rhizoma Atractylodis Macrocephalae, Fructus Choerospondiatis, Fructus Schisandrae Chinensis and Radix Glehniae.
The present invention also provides the preparation method of above-mentioned Chinese medicine composition, and when the dosage form of described Chinese medicine composition is decoction, its step is specially each crude drug is mixed in proportion, stir and pulverize, add with respect to 5 ~ 8 times of water of crude drug quality sum, decoct 2 ~ 4 hours, decoction liquor is filtered, filtering residue adds again with respect to 2 ~ 4 times of water of crude drug quality sum, again decoct 2 ~ 4 hours, decoction liquor is filtered, and filtrate is merged, heating is concentrated, obtains the decoction that crude drug concentration is 1g/ml.
The present invention also provides the preparation method of above-mentioned Chinese medicine composition, in the time that the dosage form of described Chinese medicine composition is powder, its step is specially each crude drug is mixed in proportion, stir and pulverize, add with respect to 5 ~ 8 times of water of crude drug quality sum, decoct 2 ~ 4 hours, decoction liquor is filtered, and filtering residue adds again with respect to 2 ~ 4 times of water of crude drug quality sum, again decoct 2 ~ 4 hours, decoction liquor is filtered, and filtrate is merged, and heating is concentrated except anhydrating, obtain and get dry extract, be ground into powder, dry, obtain powder.
The present invention also provides the preparation method of above-mentioned Chinese medicine composition, and in the time that the dosage form of described Chinese medicine composition is injection, its step is specially:
The first step, is mixed in proportion each crude drug, stirs and pulverizes, add with respect to 5 ~ 8 times of water of crude drug quality sum, decoct 2 ~ 4 hours, decoction liquor is filtered, filtering residue adds again with respect to 2 ~ 4 times of water of crude drug quality sum, again decocts 2 ~ 4 hours, and decoction liquor is filtered, filtrate is merged, add 1～2g active carbon, boil 10～15 minutes, after natural cooling, through centrifugal, sucking filtration, obtain filtrate, heating is concentrated into the medicinal liquid that determining alcohol is 10g/ml～15g/ml;
Second step, gets described medicinal liquid 20ml, adds successively therein 0.75ml tween 80,100mg sodium sulfite, after mix homogeneously, inject water to 100ml, and with sodium hydroxide adjust pH to 7.5, be finally sub-packed in cillin bottle, after boiling water bath heat sterilization, obtain injection.
Beneficial effect of the present invention:
Chinese medicine composition provided by the invention, at heat-clearing and toxic substances removing, when anti-inflammation, can improve the immunocompetence of milch cow, thereby improves the anti-stress of milch cow, anti-disease ability, has good effect, anti-recurrence aspect treatment cow mammary gland, have no drug resistance, noresidue, the advantage having no side effect.
Detailed description of the invention
Chinese veterinarian's traditional theory thinks, mastitis are acute mastitis, is not looseization and be inflammation of expectorant, wet, gas, blood stasis knot.Because feeding and management is not good at, the ground that lying on bed for a long time is damp and hot, steams on damp and hot poison, infringement breast; Or because of gastric heat Yong Sheng, stagnation of QI due to depression of the liver, newborn network loses smooth so that breast coagulation of QI-blood, stasis and raw carbuncle; Or because calf sucks, milk is stagnated, breast distensions etc. are former thereby cause primary disease, based on this theory, the present invention propose taking heat-clearing and toxic substances removing, dysmenorrhea invigorate blood circulation, reducing swelling and alleviating pain is as principle, treatment bovine mastitis.
In addition, be subject to cause pathogeny imcrobe infection or the parasitic invasion and attack such as antibacterial, mycete, virus when body, as dust Xi Shi escherichia coli, streptococcus pneumoniae, tubercule bacillus etc., pathogenic bacteria can secrete one and have the phagocytotic factor of the neutrophil of inhibition in host, pasteurella haemolytica can secrete one can kill cytomegalic material in body, the interference of pathogenic bacteria and so on, thus body is reduced and lose anti-infective ability.
In whole breast tissue, papillary duct is the first natural cover for defense of mammary gland tissue, and its horny layer can stop the invasion of pathogen; Its epithelial cell can be secreted the keratin with bactericidal action, and catheter proximal end can produce local antibody and cytokine.Pathogenic microorganism only has behind this defence line, road of breakthrough, could infect mammary gland tissue.In the time of breast local blood circulation obstacle, the impaired immunocompetence of mammary gland mucosal system reduces, and galactophore epithelial cell produces iron ion to be reduced in conjunction with inflammatory mediator abilities such as albumen, cytokine chemokines, host's defense peptide, argininosuccinic acid metabolites; Autocrine and paracrine reduce, and induction neutrophilic granulocyte and lymphocyte weaken to infection site effect on migration; Effect T lymphocytic emiocytosis effector reduces, and cytotoxicity weakens.The chemical barrier effect that mammary gland opposing inoculating microbe infects reduces.Meanwhile, a large amount of lymphoid cells that contain in lamina propria, the quantity such as such as mastocyte, B lymphocyte, T lymphocyte reduce; Auxiliary type T lymphocyte weakens the regulating action of B emiocytosis, causes IgA secretory volume deficiency, cannot stop the combination of the pathogen such as staphylococcus aureus and streptococcus and galactophore epithelial cell; The potentiation of the granulocytic phagocytic activity of IgG2 centering weakens, and phagocyte number reduces, cannot anti-bacteria and the epithelial combination activating complement of body of gland.And disturbance of blood circulation makes immunologically competent cell be difficult to enter mammary gland tissue, serum lysozyme activities reached reduces.Finally, hormone-cytokine-immunologic balance is destroyed, and the immune function of mammary gland reduces, and causes pathogenic bacterium to invade in a large number, breed initiation inflammation, and therefore, Chinese medicine composition provided by the invention also will play the effect that improves immunologic function.
In sum, Chinese medicine composition provided by the invention taking heat-clearing and toxic substances removing, restore menstrual flow and invigorate blood circulation, reducing swelling and alleviating pain, immunostimulant be principle, the crude drug of described Chinese medicine composition comprises Fructus Liquidambaris, Radix Glycyrrhizae, Rhizoma Polygonati, Herba Violae, the Radix Pulsatillae, Rhizoma Panacis Majoris, the Cortex Eucommiae, Folium Isatidis, Myrrha, Cortex Moutan, Folium Eriobotryae, Rhizoma Coptidis, Caulis Polygoni Multiflori, the Radix Paeoniae Alba, Rhizoma Curcumae Longae, the Radix Astragali, the Rhizoma Atractylodis Macrocephalae, Fructus Choerospondiatis, Fructus Schisandrae Chinensis and Radix Glehniae.
Preferably, described Chinese medicine composition is only made up of above-mentioned raw materials medicine.
In described Chinese medicine composition, the weight portion of each crude drug is respectively:
Fructus Liquidambaris 15 weight portion ~ 25 weight portions, Radix Glycyrrhizae 30 weight portion ~ 40 weight portions, Rhizoma Polygonati 60 weight portion ~ 70 weight portions, Herba Violae 20 weight portion ~ 30 weight portions, the Radix Pulsatillae 35 weight portion ~ 45 weight portions, Rhizoma Panacis Majoris 55 weight portion ~ 65 weight portions, the Cortex Eucommiae 40 weight portion ~ 50 weight portions, Folium Isatidis 55 weight portion ~ 65 weight portions, Myrrha 20 weight portion ~ 30 weight portions, Cortex Moutan 35 weight portion ~ 45 weight portions, Folium Eriobotryae 15 weight portion ~ 25 weight portions, Rhizoma Coptidis 50 weight portion ~ 60 weight portions, Caulis Polygoni Multiflori 35 weight portion ~ 45 weight portions, the Radix Paeoniae Alba 30 weight portion ~ 40 weight portions, Rhizoma Curcumae Longae 30 weight portion ~ 40 weight portions, the Radix Astragali 80 weight portion ~ 90 weight portions, the Rhizoma Atractylodis Macrocephalae 50 weight portion ~ 60 weight portions, Fructus Choerospondiatis 20 weight portion ~ 30 weight portions, Fructus Schisandrae Chinensis 40 weight portion ~ 50 weight portions and Radix Glehniae 30 weight portion ~ 40 weight portions.
In described Chinese medicine composition, the weight portion of each crude drug is preferably respectively:
Fructus Liquidambaris 18 weight portion ~ 22 weight portions, Radix Glycyrrhizae 33 weight portion ~ 37 weight portions, Rhizoma Polygonati 63 weight portion ~ 67 weight portions, Herba Violae 23 weight portion ~ 27 weight portions, the Radix Pulsatillae 38 weight portion ~ 42 weight portions, Rhizoma Panacis Majoris 58 weight portion ~ 62 weight portions, the Cortex Eucommiae 43 weight portion ~ 47 weight portions, Folium Isatidis 58 weight portion ~ 62 weight portions, Myrrha 23 weight portion ~ 27 weight portions, Cortex Moutan 38 weight portion ~ 42 weight portions, Folium Eriobotryae 18 weight portion ~ 22 weight portions, Rhizoma Coptidis 53 weight portion ~ 57 weight portions, Caulis Polygoni Multiflori 38 weight portion ~ 42 weight portions, the Radix Paeoniae Alba 33 weight portion ~ 37 weight portions, Rhizoma Curcumae Longae 33 weight portion ~ 37 weight portions, the Radix Astragali 83 weight portion ~ 87 weight portions, the Rhizoma Atractylodis Macrocephalae 53 weight portion ~ 57 weight portions, Fructus Choerospondiatis 23 weight portion ~ 27 weight portions, Fructus Schisandrae Chinensis 43 weight portion ~ 47 weight portions and Radix Glehniae 33 weight portion ~ 37 weight portions.
In described Chinese medicine composition, the weight portion of each crude drug is most preferably respectively:
Fructus Liquidambaris 20 weight portions, Radix Glycyrrhizae 35 weight portions, Rhizoma Polygonati 65 weight portions, Herba Violae 25 weight portions, the Radix Pulsatillae 40 weight portions, Rhizoma Panacis Majoris 60 weight portions, the Cortex Eucommiae 45 weight portions, Folium Isatidis 60 weight portions, Myrrha 25 weight portions, Cortex Moutan 40 weight portions, Folium Eriobotryae 20 weight portions, Rhizoma Coptidis 55 weight portions, Caulis Polygoni Multiflori 40 weight portions, the Radix Paeoniae Alba 35 weight portions, Rhizoma Curcumae Longae 35 weight portions, the Radix Astragali 85 weight portions, the Rhizoma Atractylodis Macrocephalae 55 weight portions, Fructus Choerospondiatis 25 weight portions, Fructus Schisandrae Chinensis 45 weight portions and Radix Glehniae 35 weight portions.
The pharmacology of each crude drug is specially:
Fructus Liquidambaris: bitter in the mouth, property is flat, returns liver, and kidney channel, has dispelling, collateral-activating, effect that diuretic stimulates the menstrual flow, for arthralgia, numbness contracture, edema distension, the cards such as the few amenorrhea of breast.
Radix Glycyrrhizae: sweet in the mouth, property is flat, enters 12 warps, has heat-clearing and toxic substances removing, is in harmonious proportion the property of medicine, the effect of expelling phlegm for arresting cough, and can spleen invigorating and in, relieving spasm to stop pain, is mainly used in the abdominal distension and anorexia of weakness of the spleen and stomach, weak; Can alleviate contracture pain in abdomen, relax the strong and toxicity of some drugs, alleviate the toxic and side effects of medicine to body or gastrointestinal is stimulated, by adding Radix Glycyrrhizae can play QI invigorating neutralization, be in harmonious proportion the effect of each component.
Rhizoma Polygonati: sweet in the mouth, property is flat, enters lung, spleen, kidney channel, has nourishing kidney lung moistening, the effect of invigorating the spleen and replenishing QI, for lung-dryness syndrome due to deficiency of YIN, dry cough expectorant is few, the polydipsia of quenching one's thirst, weakness of the spleen and stomach, deficiency of spleen-QI or deficiency of spleen-YIN, deficiency of kidney essence, soreness of the waist and knees, the cards such as early whitening of beard and hair.
Herba Violae: bitter in the mouth, pungent, cold in nature, enter the heart, Liver Channel, there is the effect of heat-clearing and toxic substances removing, removing heat from blood detumescence, be mainly used in treating the treatment of furuncle carbuncle furuncle, acute conjunctivitis, hordeolum etc. are also had to good therapeutic effect, and Herba Violae has inhibitory action in various degree to Staphyloccocus aureus, pneumobacillus, escherichia coli, alpha streptococcus, group B streptococcus, hemophilus influenza, diphtheria corynebacterium, bacillus pyocyaneus, white grapes sugar coccus, Candida albicans.
The Radix Pulsatillae: bitter in the mouth, cold in nature, enter stomach, large intestine channel, there is the effect of heat-clearing and toxic substances removing, eliminating pathogenic heat from blood to cure dysentery, be mainly used in the intestines and stomach and have damp and hot stagnant heating stomachache, dysentery pus and blood, the treatment of the symptoms such as tenesmus, hematodiarrhoea hematochezia effect to large intestine heat in blood is better, and the Radix Pulsatillae all has certain inhibitory action to dysentery bacterium, Bacillus typhi, bacillus subtilis, escherichia coli, Staphyloccocus aureus.
Rhizoma Panacis Majoris: bitter in the mouth, sweet, cold nature, enters liver, lung, stomach warp, there is tonifying the lung yin nourishing, dissipating blood stasis hemostasis, the effect of reducing swelling and alleviating pain,, cough due to consumptive disease thirsty for deficiency of both QI and YIN, excessive thirst, various hemorrhages, as haematemesis, hemoptysis, have blood in stool, hematuria, metrorrhagia, traumatic injury wound is swollen, rheumatic arthritis, stomachache, acute toothache, laryngopharynx swelling and pain, the cards such as sore and toxic.
The Cortex Eucommiae: sweet in the mouth, micro-pungent, warm in nature, enter liver, kidney channel, there is blood pressure lowering, invigorating the liver and kidney, bone and muscle strengthening, the effect of antiabortive gas, a little less than being shown in dizzy, soreness of waist and knee joint, muscles and bones flaccidity for caused by liver and kidney deficiency, card; Instability of kidney QI, frequent micturition or dripping urination, uncontrolled urination are itched, sexual impotence, anemia of pregnant woman are weak, frequent fetal movement or waist pendant pain; The cards such as chronic joint disease, bone tuberculosis, dysmenorrhea, dysfunctional uterine bleeding, chronic pelvic inflammatory disease, the leaf of the Cortex Eucommiae and skin contain the nutritional labelings such as protein, fat, vitamin and mineral.
Folium Isatidis: bitter in the mouth, property Great Cold, GUIXIN, liver, gallbladder, stomach warp, have antipyretic and detoxicated, cooling blood and dissolving purpura, effect of jaundice eliminating, the excess-heat can clearing heat in QI system dividing, can separate again the pyretic toxicity of blood system, the high febrile perspiration being mainly used in due to pestilence calentura goes out, excessive thirst taking fluids profusely; The speckle that fire-toxin is flourishing is gone mad, the symptoms such as haematemesis; The unconsciousness due to high fever of measles, rash color is purple dark; Dampness-heat in the liver and gallbladder is accumulate the jaundice of steaming; The deafness and tinnitus of liver and gall fire liter; The cards such as pharyngalgia due to fire stagnation pyretic toxicity, aphtha of the mouth and tongue, hepatitis.
Myrrha: acrid in the mouth, hardship, property is flat, and GUIXIN, liver, spleen channel, have dissipating blood stasis analgesic therapy, and effect of detumescence and promoting granulation, cures mainly obstruction of qi in the chest and cardialgia, gastralgia, dysmenorrhea amenorrhea, rheumatic arthralgia, traumatic injury, the cards such as carbuncle skin infection.
Cortex Moutan: bitter in the mouth, pungent, cold nature, enter the heart, liver, kidney channel, there is the effect of clearing away heat and cooling blood, promoting blood circulation to remove blood stasis, be mainly used in treating the epidemic febrile disease pathogenic heat attacking blood system in febrile diseases, send out speckle, tell nosebleed, hot Fu Yin of calentura later stage divides heating, deficiency of YIN osteopyrexia and fever, amenorrhea due to stagnation of blood, dysmenorrhea, carbuncle sore tumefacting virus, falls and flutter the card such as the pain of injury, rheumatism pyretic arthralgia.
Folium Eriobotryae: bitter in the mouth, cold nature, returns lung, stomach warp, has clearing away lung-heat to relieve cough, effect of stopping nausea and vomiting by lowering the adverse flow of QI, this product bitter cold, energy clearing away lung-heat, falls lung qi with cough-relieving, be usually used in treating cough due to lung-heat, abundant expectoration yellow skin, can gastric qi be fallen and preventing or arresting vomiting is contrary, the vomiting causing for gastric heat, and treatment xerostomia is quenched one's thirst, lung-wind syndrome acne vulgaris, acne
Rhizoma Coptidis: bitter in the mouth, cold in nature, enter the heart, lung, gallbladder, stomach, large intestine channel, there is the effect of heat clearing and damp drying, eliminating fire and detoxication, cooling blood for improving eyesight, be longer than the fire of removing pathogen in the heart stomach, have the disappear merit of painful abdominal mass of preventing or arresting vomiting concurrently, can be used for treating the dysphoria and insomnia of heart-fire due to prosperous, and the high fever coma of epidemic febrile disease, vexed, thirsty; Also can be used for dysentery, tenesmus due to damp and hot; The cards such as pyretic toxicity carbuncle sore, eczema, aphtha of the mouth and tongue, epidemic hemorrhagic conjunctivitis.
Caulis Polygoni Multiflori: sweet in the mouth, property is flat, and GUIXIN, Liver Channel, have nourishing blood to tranquillize the mind, and effect of dispelling wind and removing obstruction in the collateral, cures mainly insomnia and dreamful sleep, blood deficiency general pain, rheumatic arthralgia; The cards such as skin pruritus, Caulis Polygoni Multiflori can be invigorated blood circulation, stimulated the menstrual flow, blood vessel dilating, blood circulation promoting, stimulating milk secretion detumescence.
The Radix Paeoniae Alba: bitter in the mouth, acid, cool in nature, return liver, spleen channel, there is nourishing blood to suppress the hyperactive liver, slow middle pain relieving, yin fluid astringing is received effect of antiperspirant, cures mainly the breast abdomen pain over the hypochondriac region, dysentery stomachache, spontaneous sweating, fever due to yin deficiency, menoxenia, metrorrhagia, is with inferior card.
Rhizoma Curcumae Longae: bitter in the mouth, pungent, warm in nature, enter Liver Channel, there is removing blood stasis circulation of qi promoting, effect of inducing menstruation to relieve menalgia, twinges for the breast side of body, amenorrhea, lump in the abdomen, rheumatism shoulder arm pain, the cards such as tumbling and swelling, Rhizoma Curcumae Longae has antiinflammatory action.
The Radix Astragali: sweet in the mouth, warm in nature, enter spleen, lung meridian, there is tonification spleen soil, elevate a turnable ladder yang-energy, strengthening superficial resistance to stop perspiration, effect of holder skin ulcer granulation promoting, cures mainly the weak anorexia and loose stool due to deficiency of both the splenic and pulmonary QI, shortness of breath and palpitation, the visceroptosis of sinking of QI of middle-JIAO, the metrorrhagia of QI failing to control blood is had blood in stool, chronic diarrhea proctoptosis; The spontaneous sweating of exterior deficiency, the cards such as insufficiency of vital energy and blood.The Radix Astragali strengthens heart contraction ability, can improve the blood circulation of whole body, can coronary artery dilator, kidney blood vessel and Rhizoma Bistortae peripheral vessel, make cutaneous circulation vigorous; Can improve the nonspecific immunity power of body, protect the liver and fall hypoglycemic effect, staphylococcus, blood dissolubility streptococcus, Diplococcus pneumoniae, dysentery bacterium etc. are had to inhibitory action, the Radix Astragali can appreciable impact splenocyte Information Conduction, realizes immunoregulation effect.
The Rhizoma Atractylodis Macrocephalae: sweet in the mouth, hardship, warm in nature, enter spleen, stomach warp, there is spleen reinforcing promoting the circulation of QI, dampness diuretic, strengthening superficial resistance to stop perspiration, the effect that activating the spleen is moisturized, is mainly used in the feeling of fullness diarrhoea due to dysfunction of the spleen in transportation, QI failing to transform into body fluid, phlegm retention edema; The vexed lazy speech of deficiency of QI in middle-JIAO, metrorrhagia and metrostaxis, proctoptosis with chronic dysentery; The cards such as solid in the playing of deficiency of the spleen causing weakness of QI, sweating due to debility spontaneous perspiration.
Fructus Choerospondiatis: sweet in the mouth, acid, property is flat, has promoting flow of QI and blood, nourishes heart, and calms the nerves, and resists myocardial ischemia, and the effects such as cardiac function protecting, cure mainly qi depression to blood stasis, and the thoracic obstruction is had a pain, shortness of breath and palpitation, the cards such as irritability.
Fructus Schisandrae Chinensis: warm in nature, sour in the mouth, sweet, returns lung, the heart, kidney channel, has convergence astringent or styptic treatment for spontaneous sweating, supplementing QI for promoting the production of body fluid, effect of kidney calming, for chronic cough dyspnea due to deficiency, emission, enuresis frequent micturition, incessant chronic diarrhea, spontaneous perspiration, night sweat, Tianjin wound is thirsty, the deficient pulse of losing heart, the card such as interior-heat is quenched one's thirst, palpitation and insomnia.
Radix Glehniae: sweet in the mouth, micro-hardship, cold nature, has nourishing YIN and clearing away lung-heat, the effect of reinforcing stomach reg fluid, for cards such as lung-heat type cough, chronic cough expectorant blood, calentura Tianjin wound are thirsty, Rhizoma Polygonati and Radix Glehniae are combined with and can be used for treating that deficiency of YIN of the spleen and stomach, hunger but having no desire to eat, gastral cavity abdomen do not relax, dryness of the mouth and throat, the diseases such as red tongue with a little fluid.
In these raw material of Chinese medicine medicine components, contain the multiple efficacies compositions such as polysaccharide, glycoside, alkaloid, organic acid, volatility oils and fats, plant pigment, there is enhancing human body immunity function, improve animal disease resistant power, promote growth of animal and improve the effects such as breeding performonce fo animals.
Chinese medicine composition provided by the invention taking heat-clearing and toxic substances removing, restore menstrual flow and invigorate blood circulation, reducing swelling and alleviating pain, immunostimulant be principle, can anti-inflammation, promoting blood circulation and stopping pain, can improve again the immune function of ill milch cow, thereby reach treating both the principal and secondary aspects of a disease, the eliminating evil effect consolidating, this medicine is innerlich anwenden thing.
Adopt Chinese medicine to gavage decoction and gavage treatment bovine mastitis, though there is good curative effect, larger to the stimulation of milch cow, and because liver first-pass effect and the gastrointestinal metabolism of oral administration, also reduced the effective ingredient of medicine, thereby dosage is large, treatment cost is higher; And spice powder feeding, because of drug smell impact, usually causes that cow feeding amount declines, thereby affect therapeutic effect.The present invention also adopts breast perfusion method, adopts the method can avoid the shortcoming of above-mentioned administering mode, makes medicine directly act on affected part, reaches higher drug level in mammary gland, performance anti-inflammation, the therapeutical effect of raising immune function.
Therefore, Chinese medicine composition dosage form of the present invention is decoction, powder and injection.
Above-mentioned Chinese medicine composition is passed through injection for curing by the present invention, can stimulate lymphocytic propagation, and by the effect of the composition such as antibody and complement, the phagocytic power of neutrophil cell is further strengthened, significantly improve the phagocytic activity of the neutrophil cell of ill milch cow, thereby improved its immunity.
The present invention also provides the preparation method of above-mentioned Chinese medicine composition, in the time that the dosage form of described Chinese medicine composition is decoction, is specially:
Each crude drug is mixed in proportion, stir and pulverize, add with respect to 5 ~ 8 times of water of crude drug quality sum, decoct 2 ~ 4 hours, decoction liquor is filtered, filtering residue adds again with respect to 2 ~ 4 times of water of crude drug quality sum, again decoct 2 ~ 4 hours, decoction liquor is filtered, and filtrate is merged, heating is concentrated, obtains the decoction that crude drug concentration is 1g/ml.
The direct gavage of decoction prepared by the present invention is taken to milch cow.
The present invention also provides the preparation method of above-mentioned Chinese medicine composition, in the time that the dosage form of described Chinese medicine composition is powder, is specially each crude drug is mixed in proportion, stir and pulverize, add with respect to 5 ~ 8 times of water of crude drug quality sum, decoct 2 ~ 4 hours, decoction liquor is filtered, and filtering residue adds again with respect to 2 ~ 4 times of water of crude drug quality sum, again decocts 2 ~ 4 hours, decoction liquor is filtered, and filtrate is merged, and heating is concentrated except anhydrating, obtain and get dry extract, be ground into powder, dry, obtain powder.
Powder prepared by the present invention is admixed in feedstuff, takes for milch cow.
The present invention also provides the preparation method of above-mentioned Chinese medicine composition, in the time that the dosage form of described Chinese medicine composition is injection, is specially:
The first step, is mixed in proportion each crude drug, stirs and pulverizes, add with respect to 5 ~ 8 times of water of crude drug quality sum, decoct 2 ~ 4 hours, decoction liquor is filtered, filtering residue adds again with respect to 2 ~ 4 times of water of crude drug quality sum, again decocts 2 ~ 4 hours, and decoction liquor is filtered, filtrate is merged, add 1～2g active carbon, boil 10～15 minutes, after natural cooling, through centrifugal, sucking filtration, obtain filtrate, heating is concentrated into the medicinal liquid that determining alcohol is 10g/ml～15g/ml;
Second step, gets described medicinal liquid 20ml, adds successively therein 0.75ml tween 80,100mg sodium sulfite, after mix homogeneously, inject water to 100ml, and with sodium hydroxide adjust pH to 7.5, be finally sub-packed in cillin bottle, after boiling water bath heat sterilization, obtain injection.
Below adopt embodiment to describe embodiments of the present invention in detail, to the present invention, how application technology means solve technical problem whereby, and the implementation procedure of reaching technique effect can fully understand and implement according to this.
Embodiment 1 Chinese medicine injection 1
By Fructus Liquidambaris 20g, Radix Glycyrrhizae 35g, Rhizoma Polygonati 65g, Herba Violae 25g, Radix Pulsatillae 40g, Rhizoma Panacis Majoris 60g, Cortex Eucommiae 45g, Folium Isatidis 60g, Myrrha 25g, Cortex Moutan 40g, Folium Eriobotryae 20g, Rhizoma Coptidis 55g, Caulis Polygoni Multiflori 40g, Radix Paeoniae Alba 35g, Rhizoma Curcumae Longae 35g, Radix Astragali 85g, Rhizoma Atractylodis Macrocephalae 55g, Fructus Choerospondiatis 25g, Fructus Schisandrae Chinensis 45g and Radix Glehniae 35g mix, stir and pulverize, add 5.2kg water, decoct 4 hours, decoction liquor is filtered, filtering residue adds 2.5kg water again, again decoct 4 hours, decoction liquor is filtered, filtrate is merged, add 2g active carbon, boil 15 minutes, after natural cooling, through centrifugal, sucking filtration, obtain filtrate, heating is concentrated into the medicinal liquid that determining alcohol is 15g/ml, get this medicinal liquid 20ml, add successively therein 0.75ml tween 80, 100mg sodium sulfite, after mix homogeneously, inject water to 100ml, and with sodium hydroxide adjust pH to 7.5, finally be sub-packed in cillin bottle, after boiling water bath heat sterilization, obtain injection 1.
The toxicity test of embodiment 2 Chinese medicines 1
50 white mice are divided into 5 groups at random, 10 every group, and ensure male and female half and half.Route of administration is lumbar injection, tests the dosage range that draws Rhizoma Coptidis Pu injection 0% and 100% animal dead in 4000～12000mg/kg body weight by prerun, is divided into four dosage groups and a matched group, and between group, dose ratio is 1: 0.8.Dose for oral use is respectively 7680mg/kg, 9600mg/kg, 12000mg/kg, 15000mg/kg, and lumbar injection dosage is respectively with dose for oral use, the normal saline of matched group lumbar injection dosage (0.4mL).Experimental period is 7d, observes animal clinical manifestation and death condition.
After administration, respectively organize white mice outward appearance hair color light, behavioral activity is normal, occur without abnormal pathologic neural reflex, in seven days, well, other Novel presentation is seen at end in feed, body weight change is no significant difference and none animal dead compared with water matched group, cannot measure the LD of test white mice
50, LD
50being greater than 5000mg/kg is actual innocuous substance, illustrates that this Chinese medicine composition is safe.
Embodiment 3 Chinese medicine injections 2
By Fructus Liquidambaris 15g, Radix Glycyrrhizae 30g, Rhizoma Polygonati 60g, Herba Violae 20g, Radix Pulsatillae 35g, Rhizoma Panacis Majoris 55g, Cortex Eucommiae 40g, Folium Isatidis 55g, Myrrha 20g, Cortex Moutan 35g, Folium Eriobotryae 15g, Rhizoma Coptidis 50g, Caulis Polygoni Multiflori 35g, Radix Paeoniae Alba 30g, Rhizoma Curcumae Longae 30g, Radix Astragali 80g, Rhizoma Atractylodis Macrocephalae 50g, Fructus Choerospondiatis 20g, Fructus Schisandrae Chinensis 40g and Radix Glehniae 30g mix, stir and pulverize, add 5kg water, decoct 4 hours, decoction liquor is filtered, filtering residue adds 2.5kg water again, again decoct 4 hours, decoction liquor is filtered, filtrate is merged, add 2g active carbon, boil 15 minutes, after natural cooling, through centrifugal, sucking filtration, obtain filtrate, heating is concentrated into the medicinal liquid that determining alcohol is 15g/ml, get this medicinal liquid 20ml, add successively therein 0.75ml tween 80, 100mg sodium sulfite, after mix homogeneously, inject water to 100ml, and with sodium hydroxide adjust pH to 7.5, finally be sub-packed in cillin bottle, after boiling water bath heat sterilization, obtain injection 2.
Embodiment 4 Chinese medicine injections 3
By Fructus Liquidambaris 25g, Radix Glycyrrhizae 40g, Rhizoma Polygonati 70g, Herba Violae 30g, Radix Pulsatillae 45g, Rhizoma Panacis Majoris 65g, Cortex Eucommiae 50g, Folium Isatidis 65g, Myrrha 30g, Cortex Moutan 45g, Folium Eriobotryae 25g, Rhizoma Coptidis 60g, Caulis Polygoni Multiflori 35 weight portions ~ 45g, Radix Paeoniae Alba 40g, Rhizoma Curcumae Longae 40g, Radix Astragali 90g, Rhizoma Atractylodis Macrocephalae 60g, Fructus Choerospondiatis 30g, Fructus Schisandrae Chinensis 50g and Radix Glehniae 40g mix, stir and pulverize, add 6kg water, decoct 4 hours, decoction liquor is filtered, filtering residue adds 2.5kg water again, again decoct 4 hours, decoction liquor is filtered, filtrate is merged, add 2g active carbon, boil 15 minutes, after natural cooling, through centrifugal, sucking filtration, obtain filtrate, heating is concentrated into the medicinal liquid that determining alcohol is 10g/ml, get this medicinal liquid 20ml, add successively therein 0.75ml tween 80, 100mg sodium sulfite, after mix homogeneously, inject water to 100ml, and with sodium hydroxide adjust pH to 7.5, finally be sub-packed in cillin bottle, after boiling water bath heat sterilization, obtain injection 3.
Embodiment 5 Chinese medicine decoction 1
By Fructus Liquidambaris 20g, Radix Glycyrrhizae 35g, Rhizoma Polygonati 65g, Herba Violae 25g, Radix Pulsatillae 40g, Rhizoma Panacis Majoris 60g, Cortex Eucommiae 45g, Folium Isatidis 60g, Myrrha 25g, Cortex Moutan 40g, Folium Eriobotryae 20g, Rhizoma Coptidis 55g, Caulis Polygoni Multiflori 40g, Radix Paeoniae Alba 35g, Rhizoma Curcumae Longae 35g, Radix Astragali 85g, Rhizoma Atractylodis Macrocephalae 55g, Fructus Choerospondiatis 25g, Fructus Schisandrae Chinensis 45g and Radix Glehniae 35g mix, stir and pulverize, add 5.2kg water, decoct 4 hours, decoction liquor is filtered, filtering residue adds 2.5kg water again, again decoct 4 hours, decoction liquor is filtered, filtrate is merged, heating is concentrated, obtain the decoction that crude drug concentration is 1g/ml.
Embodiment 6 Chinese powder medicines 1
By Fructus Liquidambaris 20g, Radix Glycyrrhizae 35g, Rhizoma Polygonati 65g, Herba Violae 25g, Radix Pulsatillae 40g, Rhizoma Panacis Majoris 60g, Cortex Eucommiae 45g, Folium Isatidis 60g, Myrrha 25g, Cortex Moutan 40g, Folium Eriobotryae 20g, Rhizoma Coptidis 55g, Caulis Polygoni Multiflori 40g, Radix Paeoniae Alba 35g, Rhizoma Curcumae Longae 35g, Radix Astragali 85g, Rhizoma Atractylodis Macrocephalae 55g, Fructus Choerospondiatis 25g, Fructus Schisandrae Chinensis 45g and Radix Glehniae 35g mix, stir and pulverize, add 5.2kg water, decoct 4 hours, decoction liquor is filtered, filtering residue adds 2.5kg water again, again decoct 4 hours, decoction liquor is filtered, heating is concentrated except anhydrating, obtain and get dry extract, be ground into powder, dry, obtain powder 1.
Embodiment 7 grouping of animal experiment experimental animal and medications
Chose for 173 4 ~ 6 years old age of the first year in certain cattle farm, 4 ~ 5 months puerperal, detect the milch cow that suffers from mastitis through CMT, be divided at random treatment group and matched group, 113 for the treatment of group milch cows, 50 of western medicine group milch cows, suffer from Niu Zuowei blank group for another 10, do not adopt any Drug therapy, western medicine group adopts blue or green, streptomycin breast injects treatment, penicillin sodium 800,000 units, the intramuscular injection of streptomycin 500,000 units, every day 3 times, be 7 days the course for the treatment of, treatment group is all poured into injection 40 ml prepared by the embodiment of the present invention 1 in affected part, 2 times/d, medication 7d, every day 2 times, sooner or later respectively once, be 7 days the course for the treatment of, raise by special messenger for three groups, uniform based daily ration standard, feed every day 3 times, freely drink water, motion.
The collection of sample
Blood sample
Jugular vein gathers the blood (before sampling, blood position is got in sterilization) for the treatment of group and matched group milch cow, and every cattle is approximately got 15 ml, static under room temperature.
Breast sample
The milch cow of choice experiment group and treatment group, from 6:00, gathers milk every 5 h, gets altogether each 20 ml (sampling Qian Duiru district is cleaned, and then uses 70% ethanol disinfection breast district, discards 2 milk) 3 times.
The processing of sample and mensuration
Blood sample
The blood of collection is placed in to (General Requirements is no more than 20 DEG C) natural coagulation 1～2h under room temperature, ice bath 30min, the centrifugal 10 min separation of serum of 3000 r/min, 4 DEG C of preservations, to be measured.
As longer in detection time, sample is placed in to-80 DEG C of refrigerators and preserve, for subsequent use.The IL-8 and the TNF-α radioimmunological kit that adopt Beijing China English biotechnology research to produce are measured blood sample.
Breast sample
By gather milk in centrifugal 5 min of 3000 r/min; Remove upper strata butterfat, be placed in centrifugal 10 min of 16000 r/min high speed centrifuge; Get 4 DEG C of preservations of supernatant (as longer in detection time, sample to be placed in to-80 DEG C of refrigerators and to preserve), to be measured.The IL-8 and the TNF-α radioimmunological kit that adopt Beijing China English biotechnology research to produce are measured newborn sample.
Immunologic function detects
Mastitis is suffered from cattle and is carried out jugular vein blood sampling before raising respectively at morning test is forward and backward, and after the centrifugal 10min of 3 000r/min ,-20 DEG C of preservations are to be measured.The immune indexes of measuring has IgA, IgG and IgM.
Mastitis inspection
Test agent: Shanghai subclinical mastitis quick diagnosis liquid (SMT stock solution), Shanghai Dairy Research Institute, is provided by milch cow center, Shanghai;
Using method: discard formerly milk, milk is clamp-oned to diagnosis dish; Diagnosis dish tilts 45 DEG C to discard unnecessary milk, makes to retain about milk sample 2m1 in diagnosis dish; Every sample adds 2m1 Shanghai subclinical mastitis quick diagnosis liquid; Holding diagnosis dish makes level and shakes clockwise; The shake of tilting is again observed for 50 seconds afterwards.
Index criterion:
-: reactant homogenizing, flow without abnormal, it is faint yellow that color is.Corresponding Ruzhong somatic number 0-20 ten thousand/ml.
±: inclination diagnosis dish, the thin wadding layer of having tried to get to the heart of a matter when reactant flows through occurs, it is yellow or slightly green that color mostly is, be judged to suspicious, corresponding Ruzhong somatic number 20-50 ten thousand/ml.
+: shake visible reactant heterogeneity, when inclination diagnosis dish, compound has obviously cotton-shaped (corresponding Ruzhong somatic number 50 ~ 800,000/ml), or there is thick wadding in the middle of when shake reactant, stop can separating again after shake, (corresponding Ruzhong somatic number 80 ~ 1,500,000/ml), reactant color is yellow green, and Gai Ru district is judged as latent mammitis.
++: when shake diagnosis dish, reactant is sticky shape, is hung on and tries to get to the heart of a matter, and color is yellow green, corresponding Ruzhong somatic number 150 ~ 5,000,000/ml.
+++: shake diagnosis dish, it is gelatin that reactant is, and edge is translucent, and color is light green or bottle green, more than corresponding Ruzhong somatic number 5,000,000/ml.
The standard of curative effect evaluation
Cure: clinical symptom disappearance, milk directly perceived is without extremely, milk yield recovers substantially, SMT method testing result lower than " ++ " for curing.
Take a turn for the better: clinical symptom relief or disappearance, have a small amount of floccule, but milk color and luster is as good as often in milk.It is normal that output fails to recover, and SMT method testing result is still higher than " ++ ".
Invalid: the each clinical symptoms in criterion does not alleviate or aggravates.
Data analysis
Utilize SPSS13. 0 statistical software to analyze, data represent by mean+SD.
Result
Each group is suffered from IL-8, the comparison of TNF-alpha content in bovine blood and milk, the results are shown in Table 1.
The each group of table 1 is suffered from IL-8, the comparison of TNF-alpha content in bovine blood and milk
In the pathogenic process of mastitis, along with aggravation IL-8, the TNF-α of inflammation all significantly increase, by the comparison for the treatment of group of the present invention and matched group, can find out that the content difference that Chinese medicine composition of the present invention can obviously reduce IL-8 and TNF-α has statistical significance (P<0.01), prove, the elimination of mastitis inflammation is had to remarkable result.
Each group is suffered from the impact of immune indexes in bovine blood, the results are shown in Table 2.
The each group of table 2 is suffered from the impact of immune indexes in bovine blood
As can be seen from Table 2, adopt Chinese medicine composition of the present invention obviously to improve immune indexes, compared with matched group, IgM, IgA, IgG difference have statistical significance (P<0.05).
The comparison of two groups for the treatment of curative effects, the results are shown in Table 3.
Table 3 liang group treatment curative effect comparison
As can be seen from Table 3, the present invention is better than western medicine group in the effect aspect treatment bovine mastitis.
All above-mentioned these intellectual properties of primary enforcement, do not set restriction this new product of other forms of enforcement and/or new method.Those skilled in the art will utilize this important information, and foregoing amendment, to realize similar implementation status.But all modifications or transformation belong to the right of reservation based on new product of the present invention.
The above, be only preferred embodiment of the present invention, is not the restriction of the present invention being made to other form, and any those skilled in the art may utilize the technology contents of above-mentioned announcement to be changed or be modified as the equivalent embodiment of equivalent variations.But every technical solution of the present invention content that do not depart from, any simple modification, equivalent variations and the remodeling above embodiment done according to technical spirit of the present invention, still belong to the protection domain of technical solution of the present invention.
Claims (8)
1. a Chinese medicine composition that is used for the treatment of bovine mastitis with immunologic function, is characterized in that: the crude drug of described Chinese medicine composition comprises Fructus Liquidambaris, Radix Glycyrrhizae, Rhizoma Polygonati, Herba Violae, the Radix Pulsatillae, Rhizoma Panacis Majoris, the Cortex Eucommiae, Folium Isatidis, Myrrha, Cortex Moutan, Folium Eriobotryae, Rhizoma Coptidis, Caulis Polygoni Multiflori, the Radix Paeoniae Alba, Rhizoma Curcumae Longae, the Radix Astragali, the Rhizoma Atractylodis Macrocephalae, Fructus Choerospondiatis, Fructus Schisandrae Chinensis and Radix Glehniae;
In described Chinese medicine composition, the weight portion of each crude drug is respectively: Fructus Liquidambaris 15 weight portion ~ 25 weight portions, Radix Glycyrrhizae 30 weight portion ~ 40 weight portions, Rhizoma Polygonati 60 weight portion ~ 70 weight portions, Herba Violae 20 weight portion ~ 30 weight portions, the Radix Pulsatillae 35 weight portion ~ 45 weight portions, Rhizoma Panacis Majoris 55 weight portion ~ 65 weight portions, the Cortex Eucommiae 40 weight portion ~ 50 weight portions, Folium Isatidis 55 weight portion ~ 65 weight portions, Myrrha 20 weight portion ~ 30 weight portions, Cortex Moutan 35 weight portion ~ 45 weight portions, Folium Eriobotryae 15 weight portion ~ 25 weight portions, Rhizoma Coptidis 50 weight portion ~ 60 weight portions, Caulis Polygoni Multiflori 35 weight portion ~ 45 weight portions, the Radix Paeoniae Alba 30 weight portion ~ 40 weight portions, Rhizoma Curcumae Longae 30 weight portion ~ 40 weight portions, the Radix Astragali 80 weight portion ~ 90 weight portions, the Rhizoma Atractylodis Macrocephalae 50 weight portion ~ 60 weight portions, Fructus Choerospondiatis 20 weight portion ~ 30 weight portions, Fructus Schisandrae Chinensis 40 weight portion ~ 50 weight portions and Radix Glehniae 30 weight portion ~ 40 weight portions.
2. Chinese medicine composition as claimed in claim 1, is characterized in that: described Chinese medicine composition dosage form is decoction, powder and injection.
3. a Chinese medicine composition decoction that is used for the treatment of bovine mastitis with immunologic function, is characterized in that: the crude drug in described decoction comprises Fructus Liquidambaris, Radix Glycyrrhizae, Rhizoma Polygonati, Herba Violae, the Radix Pulsatillae, Rhizoma Panacis Majoris, the Cortex Eucommiae, Folium Isatidis, Myrrha, Cortex Moutan, Folium Eriobotryae, Rhizoma Coptidis, Caulis Polygoni Multiflori, the Radix Paeoniae Alba, Rhizoma Curcumae Longae, the Radix Astragali, the Rhizoma Atractylodis Macrocephalae, Fructus Choerospondiatis, Fructus Schisandrae Chinensis and Radix Glehniae;
In described Chinese medicine composition decoction, the weight portion of each crude drug is respectively: Fructus Liquidambaris 15 weight portion ~ 25 weight portions, Radix Glycyrrhizae 30 weight portion ~ 40 weight portions, Rhizoma Polygonati 60 weight portion ~ 70 weight portions, Herba Violae 20 weight portion ~ 30 weight portions, the Radix Pulsatillae 35 weight portion ~ 45 weight portions, Rhizoma Panacis Majoris 55 weight portion ~ 65 weight portions, the Cortex Eucommiae 40 weight portion ~ 50 weight portions, Folium Isatidis 55 weight portion ~ 65 weight portions, Myrrha 20 weight portion ~ 30 weight portions, Cortex Moutan 35 weight portion ~ 45 weight portions, Folium Eriobotryae 15 weight portion ~ 25 weight portions, Rhizoma Coptidis 50 weight portion ~ 60 weight portions, Caulis Polygoni Multiflori 35 weight portion ~ 45 weight portions, the Radix Paeoniae Alba 30 weight portion ~ 40 weight portions, Rhizoma Curcumae Longae 30 weight portion ~ 40 weight portions, the Radix Astragali 80 weight portion ~ 90 weight portions, the Rhizoma Atractylodis Macrocephalae 50 weight portion ~ 60 weight portions, Fructus Choerospondiatis 20 weight portion ~ 30 weight portions, Fructus Schisandrae Chinensis 40 weight portion ~ 50 weight portions and Radix Glehniae 30 weight portion ~ 40 weight portions.
4. a Chinese medicine composition composition powders that is used for the treatment of bovine mastitis with immunologic function, is characterized in that: the crude drug in described powder comprises Fructus Liquidambaris, Radix Glycyrrhizae, Rhizoma Polygonati, Herba Violae, the Radix Pulsatillae, Rhizoma Panacis Majoris, the Cortex Eucommiae, Folium Isatidis, Myrrha, Cortex Moutan, Folium Eriobotryae, Rhizoma Coptidis, Caulis Polygoni Multiflori, the Radix Paeoniae Alba, Rhizoma Curcumae Longae, the Radix Astragali, the Rhizoma Atractylodis Macrocephalae, Fructus Choerospondiatis, Fructus Schisandrae Chinensis and Radix Glehniae;
In described Chinese medicine composition composition powders, the weight portion of each crude drug is respectively: Fructus Liquidambaris 15 weight portion ~ 25 weight portions, Radix Glycyrrhizae 30 weight portion ~ 40 weight portions, Rhizoma Polygonati 60 weight portion ~ 70 weight portions, Herba Violae 20 weight portion ~ 30 weight portions, the Radix Pulsatillae 35 weight portion ~ 45 weight portions, Rhizoma Panacis Majoris 55 weight portion ~ 65 weight portions, the Cortex Eucommiae 40 weight portion ~ 50 weight portions, Folium Isatidis 55 weight portion ~ 65 weight portions, Myrrha 20 weight portion ~ 30 weight portions, Cortex Moutan 35 weight portion ~ 45 weight portions, Folium Eriobotryae 15 weight portion ~ 25 weight portions, Rhizoma Coptidis 50 weight portion ~ 60 weight portions, Caulis Polygoni Multiflori 35 weight portion ~ 45 weight portions, the Radix Paeoniae Alba 30 weight portion ~ 40 weight portions, Rhizoma Curcumae Longae 30 weight portion ~ 40 weight portions, the Radix Astragali 80 weight portion ~ 90 weight portions, the Rhizoma Atractylodis Macrocephalae 50 weight portion ~ 60 weight portions, Fructus Choerospondiatis 20 weight portion ~ 30 weight portions, Fructus Schisandrae Chinensis 40 weight portion ~ 50 weight portions and Radix Glehniae 30 weight portion ~ 40 weight portions.
5. a Chinese medicine composition composition injection that is used for the treatment of bovine mastitis with immunologic function, is characterized in that: the crude drug in described injection comprises Fructus Liquidambaris, Radix Glycyrrhizae, Rhizoma Polygonati, Herba Violae, the Radix Pulsatillae, Rhizoma Panacis Majoris, the Cortex Eucommiae, Folium Isatidis, Myrrha, Cortex Moutan, Folium Eriobotryae, Rhizoma Coptidis, Caulis Polygoni Multiflori, the Radix Paeoniae Alba, Rhizoma Curcumae Longae, the Radix Astragali, the Rhizoma Atractylodis Macrocephalae, Fructus Choerospondiatis, Fructus Schisandrae Chinensis and Radix Glehniae;
In described Chinese medicine composition composition injection, the weight portion of each crude drug is respectively: Fructus Liquidambaris 15 weight portion ~ 25 weight portions, Radix Glycyrrhizae 30 weight portion ~ 40 weight portions, Rhizoma Polygonati 60 weight portion ~ 70 weight portions, Herba Violae 20 weight portion ~ 30 weight portions, the Radix Pulsatillae 35 weight portion ~ 45 weight portions, Rhizoma Panacis Majoris 55 weight portion ~ 65 weight portions, the Cortex Eucommiae 40 weight portion ~ 50 weight portions, Folium Isatidis 55 weight portion ~ 65 weight portions, Myrrha 20 weight portion ~ 30 weight portions, Cortex Moutan 35 weight portion ~ 45 weight portions, Folium Eriobotryae 15 weight portion ~ 25 weight portions, Rhizoma Coptidis 50 weight portion ~ 60 weight portions, Caulis Polygoni Multiflori 35 weight portion ~ 45 weight portions, the Radix Paeoniae Alba 30 weight portion ~ 40 weight portions, Rhizoma Curcumae Longae 30 weight portion ~ 40 weight portions, the Radix Astragali 80 weight portion ~ 90 weight portions, the Rhizoma Atractylodis Macrocephalae 50 weight portion ~ 60 weight portions, Fructus Choerospondiatis 20 weight portion ~ 30 weight portions, Fructus Schisandrae Chinensis 40 weight portion ~ 50 weight portions and Radix Glehniae 30 weight portion ~ 40 weight portions.
6. the preparation method of Chinese medicine composition described in claim 1 or 2, is characterized in that: when the dosage form of described Chinese medicine composition is decoction, its step is specially each crude drug is mixed in proportion, stir and pulverize, add with respect to 5 ~ 8 times of water of crude drug quality sum, decoct 2 ~ 4 hours, decoction liquor is filtered, filtering residue adds again with respect to 2 ~ 4 times of water of crude drug quality sum, again decoct 2 ~ 4 hours, decoction liquor is filtered, and filtrate is merged, heating is concentrated, obtains the decoction that crude drug concentration is 1g/ml.
7. the preparation method of Chinese medicine composition described in claim 1 or 2, it is characterized in that: in the time that the dosage form of described Chinese medicine composition is powder, its step is specially each crude drug is mixed in proportion, stir and pulverize, add with respect to 5 ~ 8 times of water of crude drug quality sum, decoct 2 ~ 4 hours, decoction liquor is filtered, and filtering residue adds again with respect to 2 ~ 4 times of water of crude drug quality sum, again decoct 2 ~ 4 hours, decoction liquor is filtered, and filtrate is merged, and heating is concentrated except anhydrating, obtain and get dry extract, be ground into powder, dry, obtain powder.
8. the preparation method of Chinese medicine composition described in claim 1 or 2, is characterized in that: in the time that the dosage form of described Chinese medicine composition is injection, its step is specially:
The first step, is mixed in proportion each crude drug, stirs and pulverizes, add with respect to 5 ~ 8 times of water of crude drug quality sum, decoct 2 ~ 4 hours, decoction liquor is filtered, filtering residue adds again with respect to 2 ~ 4 times of water of crude drug quality sum, again decocts 2 ~ 4 hours, and decoction liquor is filtered, filtrate is merged, add 1～2g active carbon, boil 10～15 minutes, after natural cooling, through centrifugal, sucking filtration, obtain filtrate, heating is concentrated into the medicinal liquid that crude drug concentration is 10g/ml～15g/ml;
Second step, gets described medicinal liquid 20ml, adds successively therein 0.75ml tween 80,100mg sodium sulfite, after mix homogeneously, inject water to 100ml, and with sodium hydroxide adjust pH to 7.5, be finally sub-packed in cillin bottle, after boiling water bath heat sterilization, obtain injection.
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| CN106267193A (en) * | 2016-09-27 | 2017-01-04 | 中国农业科学院兰州畜牧与兽药研究所 | A kind of for infusion of medicine agent treating bovine mastitis and its preparation method and application |
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