KR20140148096A - INNATE IMMUNE ENHANCING AND ANTIVIRAL COMPOSITION COMPRISING EXTRACT OF Cimicifuga heracleifolia Komarow - Google Patents

Abstract

The present invention relates to an innate immunity-enhancing and antiviral composition containing an extract of Cimicifuga heracleifolia Komarov, and to a method for enhancing animal innate immunity and antiviral activity, the method comprising administering an extract of Cimicifuga heracleifolia Komarov to a non-human animal in need of innate immunity enhancement. The innate immunity-enhancing and antiviral composition according to the present invention exhibits antiviral activity and innate immunity-enhancing efficacy, and thus can be applied for preventing and treating bacterial and viral infectious diseases, and can be widely used as a medicine or functional health food for enhancing and regulating immunity of immunodeficient or immunosuppressed patients, and as an innate immunity-enhancing and antiviral feed having a purpose of enhancing disease-resistance of animals. Furthermore, the innate immunity-enhancing and antiviral composition according to the present invention hardly induces toxicity or side effects, thus can be safely used despite long-term use for preventive purposes.

Description

FIELD OF THE INVENTION [0001] The present invention relates to an innate immunity enhancing and antiviral composition comprising an equine extract,

The present invention relates to a congenital immune enhancement and antiviral composition, and more particularly to a congenital immunostimulating and antiviral composition comprising an equine extract which can be used as a medicament, a health functional food and a feed composition.

Immunity can be divided into innate immunity, which is born from birth, and acquired immunity, which is obtained by adapting to life. Congenital immunity is also called "natural immunity" and is characterized by nonspecific responses to antigens and no special memory effect.

Among the in vivo innate defense immune systems, the most important research field is the field of congenital immunity induced by interferon. Such activation of interferon-mediated immunity can be a fundamental preventive measure against various infectious pathogens. Therefore, studies on the interferon activation mechanism and development of immunoregulatory agents capable of inducing interferon have been actively conducted. In addition, immune factors (inflammatory cytokines) are secreted as a defense mechanism against congenital immune diseases caused by pathogenic infections, and inflammatory responses are induced by these factors to protect against pathogens. Therefore, induction of inflammatory response at an appropriate level may be a preventive and therapeutic method for various infectious disease pathogens, and development of an immunity enhancer that can induce the inflammatory response is needed.

Virus means a toxic substance in Latin and refers to a group of infectious pathogenic particles that pass through bacterial filter paper (0.22 μm). Viruses can be classified as bacteriophages, plant viruses, and animal viruses according to the type of host cells. DNA viruses and RNA viruses can be classified according to the type of nucleic acid. Recently, various virus diseases such as H1N1, AI, and foot-and-mouth disease have caused a great social problem, and the concern about effective measures for viral diseases has raised a great interest in society.

Currently, vaccination is the best way to prevent viral diseases. Nevertheless, in the case of diseases caused by viruses, the efficiency of vaccines due to the generation of many virus serotypes (subtypes) is important. The development and dissemination of antiviral inhibitors that can overcome the problems of these vaccines is an important issue. For this purpose, a preventive agent that enhances immunity of an individual animal by stimulating the in vivo innate immune system, Can be an important method of drug development.

Amantadine and rimantadine are two typical antiviral agents that inhibit the proliferation of influenza virus. However, these two antiviral agents are effective only for the serotype A influenza virus and the serotype B It has been confirmed that it is not effective against influenza virus. In addition, amantadine and rimantadine have been found to have a disadvantage in that the mutant virus, which does not affect the ion channel function of the influenza virus M2 protein, appears very easily. To overcome this drawback, zanamivir and oseltamivir have been developed as antiviral agents effective against all 16 serotype A influenza viruses and serotype B influenza viruses. However, there is a disadvantage that Zanamivir should be administered by inhalation and intravenous injection. Ocelaminivir can be administered orally, but it is pointed out as a disadvantage due to recent reports of the emergence of resistant virus and side effects such as vomiting and dizziness when administered orally.

In addition, vaccines against Newcastle Disease virus are classified into virulence vaccine and Sadox vaccine. It is known that the most widely used Newcastle disease virulence vaccine, B1 strain and La Sota strain (including Clone strain) The multivitamins combined vaccine oil vaccine, a Newcastle Disease vaccine that is used globally, can prevent three or more diseases at the same time by a single vaccination. However, in the case of laying hens farms, cases of Newcastle disease caused by immunity reduction are increasing.

In addition, the main control method of vesicular stomatitis virus is the complete treatment of the disease, so as with foot-and-mouth disease, prevention and blocking prevention and eradication of susceptible domestic livestock are the best methods.

Under these circumstances, many efforts have recently been made to overcome the disadvantages of existing antiviral agents both in foreign countries and domestic ones. As one of the efforts, researches on antiviral efficacy of herbal medicine extracts and plant extracts are actively underway in Korea .

On the other hand, horse riding (Cimicifuga heracleifolia Komarow) is also called perennial herbaceous perennial herb belonging to the buttercups family, and the rootstock of equine and its plants are used for medicinal purposes. The mountains are Korea, China and Japan. In Korea, there are C. dahyrica Max., C. foetida L. and C. simplex Wormsk. The rootstock is uneven in the shape of thick nodule, 6 ~ 8 ㎝ in length, and 10 ~ 25 ㎝ in diameter. The outer surface is brown or black, with several large stem marks on the top of the rootstock, with many root residues attached.

 In one room, it is known to have the efficacy of winning, hunting, tinnitus and detoxification. The roots of horse riding include cimicifugine, salicylic acid, tannin, resin, caffeic acid, ferulic acid, cimitin, alkaloids, Glycosides, and isoferulic acid. (See, for example, Kagaku Shin-kai, Encyclopedia of Chinese Medicine, Book Publishing Co., pp. 3362-3372, 1998) Furthermore, cycloartane, (Tetracyclic triterpenoid), but the type and content of the tetracyclic triterpenoid differs depending on the kind of the origin plant. Among them, horse riding is known to have bisnaginous components and to have an epidermal action (see, for example, Haemophilia, Compendium of Herbal Medicine Textbook, Dongmyungsa, Seoul, 2000.). It has been reported that these horse riding extracts regulate insulin secretion through the formation of glutamate and the activity of glutamate dehydrogenase (GDH) (Lee, KH et al., Mol. Cells. 17 (3) 514, 2004). Also, it is known that visnazin, which is known as an effective ingredient of horse riding, suppresses vascular smooth muscle contraction by decreasing blood pressure and inhibiting calcium influx (Korean Journal of Pharmacology and Toxicology, Seoul, 2000) Duarte, J. et al., Eur J Pharmacol., 286 (2), pp 115-122, 1995). However, there is still no report on the immune enhancement efficacy or antiviral efficacy of horse riding.

Therefore, there is still a need for a composition using an equine extract which can overcome the disadvantages of existing antiviral agents and exhibit excellent immunity enhancing effect and antiviral activity with little toxicity and side effects.

The present inventors completed the present invention by confirming that the extract of horse riding can activate macrophages which are the main cells of innate immunity and inhibit the proliferation of various viruses. The present invention relates to a method for preventing and treating various viruses or bacterial infectious diseases while ensuring long-term stability with little toxicity and side effects, and also to provide an immunological enhancer including an equine extract which can be effectively used for a pharmaceutical composition, a health functional food, It is an object of the present invention to provide an antiviral composition and a method for enhancing immunity and antiviral activity of an animal.

One aspect of the present invention to solve the above problems provides a concomitant immunomodulatory and antiviral pharmaceutical composition comprising a horse riding extract.

Another aspect of the present invention provides a congenital immunomodulating and antiviral health functional food comprising an equine horse extract.

In addition, another aspect of the present invention provides a congenital immunostimulating and antiviral feed composition comprising an equine extract.

Yet another aspect of the present invention provides for enhancing innate immunity and enhancing antiviral activity in an animal comprising administering an equine horse extract to an animal in need of congenital immunity enhancement other than human.

In the present invention, the horse riding extract includes those extracted with water, a lower alcohol having 1 to 4 carbon atoms, and a mixed solvent thereof, preferably methanol, ethanol, or butanol. In addition, in the present invention, the above-mentioned extract includes any one of the extract obtained by the extraction treatment, the diluted or concentrated liquid of the extract, the dried product obtained by drying the extract, or the adjusted product or the purified product.

The term "antiviral" in the present invention includes, for example, influenza, avian influenza, Newcastle virus, Vesicular stomatitis virus, Cossackievirus, Enterovirus-71, Herpes simplex virus Herpes Simplex Virus, Foot and Mouth Disease (FMD), Colorado tick fever virus, Leovirus, Human Immunodeficiency Virus, and Hepatitis Virus Type B Type C, Pig Fever, Bovine Viral Diarrhea Virus, porcine reproductive and respiratory syndrome virus, porcine Ozski disease, rotavirus, parvovirus, porcine epidemic diarrhea virus, and the like.

According to the present invention, it is possible to effectively apply to the prevention and treatment of various viruses and bacterial infectious diseases by using an equine extract which exhibits excellent innate immune enhancing effect, and can contribute to enhancement of immunity.

In addition, the composition for concealed immunity enhancement and antivirus comprising the horse riding extract according to the present invention hardly causes toxicity or side effects, so that it can be safely used for prolonged use even for prophylactic purposes.

Therefore, the composition according to the present invention can be applied for the prevention and treatment of bacterial and viral infectious diseases, and can be applied to pharmaceutical compositions and health functional foods for immunity enhancement and control of immunocompromised or immunosuppressed patients, Can be widely used as an innate immunity enhancing feed composition for the purpose of enhancing disease.

Further, according to the present invention, it is possible to effectively and safely enhance the immunity of an animal by administering an equine estrogen extract to an animal necessary for innate immunity enhancement.

FIG. 1 shows experimental results of antiviral activity against PR8-gfp (Swine influenza virus) of horse-riding extract according to an embodiment of the present invention.
FIG. 2 shows the results of antiviral activity test for VSV-gfp (Vesicular stomatitis virus) of horse-riding extract according to an embodiment of the present invention.
FIG. 3 shows the results of an antiviral activity test for NDV-gfp (Newcastle disease virus) of the horse riding extract according to an embodiment of the present invention.
4 shows results of induction of interferon-beta and proinflammatory cytokine secretion induced by horse-riding extract according to an embodiment of the present invention.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings.

The present invention is characterized in that it exhibits an immunostimulating effect by using an equine extract which can activate macrophages which are the main cells of innate immunity and inhibit the multiplication of various viruses.

The horse rid extract used in the present invention can be generally formed by extraction using various solvents used for extracting natural products, for example, by one or more methods selected from the group consisting of hot water extraction, alcohol extraction and alcohol extraction .

In one embodiment, the horse riding extract comprises: adding distilled water; Hydrothermal extraction at a temperature of 20 to 130 캜, preferably about 105 캜, for 30 to 480 minutes, preferably about 150 minutes; And filtering.

The horse riding extract shows excellent antiviral activity against various viruses such as influenza virus, Newcastle disease virus, and vesicular stomatitis virus, strongly expresses immunity factor induction ability, and increases inherent immunity of an individual to inhibit infection and proliferation of virus .

In addition, the horse riding extract has little cytotoxicity or side effects, so it can be safely used for long-term use.

In the present invention, it can be applied to congenital immunity enhancing and antiviral pharmaceutical compositions including equine extracts exhibiting such effects, congenital immunity enhancing and antiviral health functional foods, congenital immunity enhancing and antiviral feed compositions, and the like .

One aspect of the present invention relates to a concomitant immunoconjugate and antiviral pharmaceutical composition comprising an equine extract.

In one embodiment, the pharmaceutical compositions for congenital immune enhancement and antiviral of the present invention further comprise a pharmaceutically acceptable carrier, excipient or diluent.

The pharmaceutically acceptable carrier, excipient or diluent which can be used in the present invention is not particularly limited so long as the effect of the present invention is not impaired, and examples thereof include fillers, extenders, binders, wetting agents, disintegrants, surfactants, lubricants, Flavoring agents, fragrances, preservatives, and the like.

Representative examples of pharmaceutically acceptable carriers, excipients or diluents include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, maltitol, starch, gelatin, glycerin, acacia rubber, alginate, calcium phosphate, calcium carbonate, calcium Methylcellulose, methylcellulose, microcrystalline cellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, mineral oil, propylene glycol, polyethylene glycol, vegetable oil, injectable Ester, witepsol, macrogol, tween 61, cacao paper, and laurie paper.

The pharmaceutical composition for congenital immune enhancement and antiviral of the present invention may be in the form selected from the group consisting of tablets, pills, powders, granules, capsules, suspensions, emulsions, syrups, aerosols, external preparations, suppositories and injections.

The preparation method of the pharmaceutical composition may be carried out according to a conventional method known in the art, and is not particularly limited.

The pharmaceutical composition for congenital immune enhancement and antiviral use of the present invention can be administered orally or parenterally. The dosage may be adjusted depending on the age, sex, weight, condition, degree of disease, drug form, May be appropriately selected, but generally about 5 to 500 mg / kg, preferably about 100 to 250 mg / kg, can be administered one to three times a day.

It will be apparent to those skilled in the art that the method of administration, dosage, route of administration, constituents, etc. of the pharmaceutical composition for congenital immune enhancement and antiviral of the present invention can be suitably selected from conventional techniques known in the art.

The pharmaceutical composition for congenital immune enhancement and antiviral of the present invention can be used for the prevention and treatment of bacterial infectious diseases or viral infectious diseases.

In particular, the pharmaceutical composition for congenital immune enhancement and antiviral of the present invention contains a horse extract which has little toxicity or side effects as an active ingredient, so that it can be safely used for prophylactic purposes for a long time.

In one embodiment, the pharmaceutical composition for congenital immune enhancement and antiviral use of the present invention may be used as an active ingredient in combination with a pharmaceutically active ingredient in addition to the horse rid extract or in combination with a pharmaceutical composition containing other active ingredient .

For example, the pharmaceutical composition for congenital immune enhancement and antiviral of the present invention can be used for preventing or treating cancer, particularly prostate, colon, lung, breast, ovary, head and neck, vulva, bladder, The present invention relates to a pharmaceutical composition for the treatment of influenza, avian influenza, avian influenza, Newcastle virus, Vesicular stomatitis virus, Cossackievirus, Enterovirus-71, Herpes Simplex virus, Foot and mouth disease (FMD), Colorado tick fever virus, reovirus, human immunodeficiency virus, and hepatitis B virus type C, swine fever virus, bovine viral diarrhea virus, porcine respiratory syndrome .Porcine reproductive and respiratory syndrome virus, porcine Ozeki disease, rotavirus, parvovirus, porcine ep idemic diarrhea virus), or a pharmaceutical composition for the treatment of autoimmune diseases, and the like.

Another aspect of the present invention relates to a healthy functional food for congenital immune enhancement and antiviral comprising an equine extract.

In one embodiment, the congenital immunomodulating and antiviral health functional food of the present invention further comprises a pharmaceutically acceptable food-aid additive.

Food-acceptable food supplementary additives that may be used in the present invention include sugars such as glucose, fructose, maltose, sucrose, dextrin, cyclodextrins, natural carbohydrates such as sugar alcohols such as xylitol, sorbitol, erythritol, Natural flavors such as martin and stevia extract, synthetic flavors such as saccharin and aspartame, colorants, pectic acid or its salts, alginic acid or its salts, organic acids, protective colloid thickeners, pH adjusting agents, stabilizers, preservatives, glycerin , Alcohols, carbonating agents, and the like.

The congenital immunostimulant and antiviral health functional food of the present invention may be in a form selected from the group consisting of powder, granule, tablet, capsule, candy, chewing gum, jelly and beverage.

The content of the horse riding extract in the healthy immunizing and antiviral health food may be appropriately selected in consideration of the shape, flavor, taste, etc. of the food, and may be 0.01 to 30% by weight Lt; / RTI >

It is obvious to a person skilled in the art that the form, composition and manufacturing method of the healthy immunocomposting and antiviral health functional food of the present invention can be suitably selected from conventional techniques known in the art.

Another aspect of the present invention relates to a congenital immunostimulating and antiviral feed composition comprising an equine extract.

The content of the horse riding extract in the composition for conferring immunity enhancement and antiviral composition may be appropriately selected according to species, age, body weight, rearing condition and the like of the feed livestock, and is preferably 0.01 to 95% by weight, 0.1 to 80% by weight.

The feed composition for congenital immunity enhancement and antiviral of the present invention can be produced according to a feed production method known in the art. For example, after mixing various feed ingredients or compound feed with the horse riding extract of the present invention, For example, a step in the form of a pellet or a step in the form of granules or the like.

It is apparent to those skilled in the art that the composition, composition, manufacturing method, feeding method, and the like of the congenital immunostimulating and antiviral feed composition of the present invention can be appropriately selected from conventional techniques known in the art.

Another aspect of the present invention relates to enhancing the innate immunity and antiviral activity of an animal, which comprises administering an equine horse extract to an animal in need of innate immune enhancement other than human.

It is clear to the ordinarily skilled artisan that the dose, administration route, administration timing, etc. of the horse riding extract in the intestinal immunity enhancement and antiviral activity enhancement of the animal of the present invention can be appropriately selected from the ordinary skill in the art .

As described above, in the present invention, it is possible to inhibit proliferation of various viruses by activating macrophages, and using the horse rid extract, which has little toxicity or side effects, in a pharmaceutical composition, a health functional food or a feed composition, Can exert a disease-strengthening effect, and can further provide an innate immunity enhancement and an antiviral activity enhancement of an animal.

Hereinafter, the present invention will be described in more detail by way of examples. However, the following examples are illustrative of the present invention, and the scope of the present invention is not limited to the following examples.

[ Example ]

1. Preparation of herbal medicine extract

The crude herbal extract was extracted with hot water at 105 ° C for 10 minutes, distilled water was extracted for 150 minutes, primary filtered at 0.45 μm and finally filtered at 0.22 μm to remove the precipitate. Subsequently, the pH was adjusted to 7.0 and 1 ml of each was dispensed into a 1.5 ml Ep-tube and stored at -20 캜 for all experiments.

2. Mouse Macrophage cell line  Antiviral activity test of horse riding extract

: The antiviral activity of horse riding extracts against various viruses, Influenza virus, Newcastle disease virus and Vesicular stomatitis virus was tested as follows.

(1) Antiviral activity test method

1) Mouse macrophage cell line Raw 264.7 was used for the experiment (8 × 10 ⁵ cells / well)

2) After cells were cultured on a 12-well TC plate, the horse rub extract prepared above (pH adjustment) was treated with DMEM supplemented with 1% FBS.

3) Negative control group was treated with DMEM supplemented with 1% FBS, and positive control was treated with Mouse IFN-B (500 units / ml).

4) PR8-gfp (MOI: 0.5), VSV-gfp (MOI: 0.5) and NDV-gfp (MOI: 0.5) were inoculated 12 hours after the horse riding treatment.

5) After 2 hours of inoculation, the inoculum was removed, washed three times with PBS, and the degree of virus infection was measured after 8-10 hours.

(2) Results of antiviral activity test

1) PR8-gfp (swine influenza virus) test results

Figure 1 shows the results of the antiviral activity test for PR8-gfp. 1 (a) shows GFP (green fluorescent protein) fluorescence image after 12 hours of infection, FIG. 1 (b) shows green fluorescent protein (GFP) fluorescence image after 24 hours of infection, 1 (d) shows the activity of the virus.

From FIG. 1, it was confirmed that the infection rate of influenza virus was remarkably decreased and the cell survival rate was also increased compared to the virus infection group as a result of pretreatment with horse riding extract.

2) Results of VSV-gfp (Vesicular stomatitis virus)

FIG. 2 shows the results of the antiviral activity test for VSV-gfp. FIG. 2 (a) shows GFP (green fluorescent protein) fluorescence image after 12 hours of infection, FIG. 2 (b) shows green fluorescent protein (GFP) fluorescence image after 24 hours of infection, , And Fig. 2 (d) shows the titer of the virus.

As can be seen from FIG. 2, as a result of pretreatment with horse riding extract, the rate of vesicular stomatitis virus infection was remarkably decreased, the virus level was decreased, and the cell survival rate was also higher than the virus infection group.

3) NDV-gfp (newcastle disease virus)

FIG. 3 shows the results of an antiviral activity test for NDV-gfp. 3 (a) shows GFP (green fluorescent protein) fluorescence image after 12 hours of infection, FIG. 3 (b) shows green fluorescent protein (GFP) fluorescence image after 24 hours of infection, And Fig. 3 (d) shows the titer of the virus through RT-PCR.

As can be seen from FIG. 3, as a result of pre-treatment with horse riding extract, the infection rate of Newcastle disease virus was remarkably decreased and the cell survival rate was also higher than that of virus infection group.

4. Mouse Macrophage cell line  Interferon-beta and Proinflammatory  Cytokine secretion induction experiment

: Experiments were conducted as follows to confirm the induction effect of the equine extract on the immune factors.

(One) Proinflammatory  Cytokine induction and interferon-beta secretion induction ( Interferon -β secretion Induction ) Experimental method

1) Mouse macrophage cell line Raw 264.7 was grown and used in the experiment.

2) Cells were cultured on a 6-well TC plate and DMEM supplemented with 1% FBS was treated with 1% concentration of the horse rid extract (pH adjusted) prepared above.

3) Negative control group was treated with DMEM supplemented with 1% FBS, and positive control group was treated with interferon-β.

4) The values of TNF-α, IL-6 and interferon-β were measured by ELISA for the results of 12 hours and 24 hours after treatment.

(2) Proinflammatory  Results of induction of cytokine and interferon-beta secretion

Fig. 4 shows the results of induction of proinflammatory cytokine and interferon-beta secretion by horse-radish extract. TNF-α is a mediator of immune and inflammatory responses that activates macrophages and induces a variety of cytokine secretion. B-cells proliferate and IL-6 activates B-cells to increase antibody production, It is an important cytokine that stimulates the immune response. In addition, interferon-beta is a cytokine secreted in response to external substances such as viruses and cancer cells, and induces an immune response by causing intracellular anti-cancer and antiviral actions.

From FIG. 4, it can be confirmed that the TNF-α, IL-6 and interferon-β-inducing ability strongly appear from the raw cells by the horse riding extract.

8. Manufacturing example

(1) Injection

The horse-shoe extract prepared in the above 1.: 100 mg

Sodium metabisulfite: 3.0 mg

Methylparaben: 0.8 mg

Propyl paraben 0.1 mg

Sterile sterilized distilled water for injection:

The above ingredients were mixed and made into a final volume of 2 ml by a conventional method, filled in an ampoule and sterilized to prepare an injection.

(2) Purification

The horse riding extract prepared in the above 1.: 200 mg

Potato starch: 100 mg

Lactose: 100 mg

Colloidal silicic acid: 16 mg

Magnesium stearate:

The ingredients were mixed and tableted according to a conventional tablet preparation method to prepare tablets.

(3) Capsules

The horse-shoe extract prepared in the above 1.: 100 mg

Lactose: 50 mg

Starch: 50 mg

Talc: 2 mg

Magnesium stearate:

The above components were mixed according to a conventional capsule manufacturing method and filled in gelatin capsules to prepare capsules.

(4) pillars

The horse riding extract prepared in the above 1.: 120 mg

Corn starch: 100 mg

Sterilized distilled water: suitable amount

The above ingredients were mixed and pelletized to spheres of appropriate size according to conventional pellet manufacturing methods to produce pellets.

(5) health functional foods

1) Health drinks

The ritual extract prepared in the above item 1 was mixed in an appropriate amount and sterilized by a drink material such as oligosaccharide (2%), liquid fructose (0.5%), sugar (2%), salt (0.5% A beverage was prepared.

2) Functional food

The functional food containing the horse riding extract was prepared by mixing the horse riding extract prepared in the above item 1 in appropriate amounts with various vitamins and minerals containing functional foods.

(6) Feed composition

The animal feed composition was mixed with an appropriate amount of the horse riding extract prepared in the above 1. to prepare a feed composition, which was then pelletized and granulated.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments or constructions. Various changes, substitutions and alterations can be made hereto without departing from the spirit and scope of the invention. It will be clear to those who have knowledge.

Claims (13)

A pharmaceutical composition for congenital immune enhancement and antiviral comprising horse riding extract.
The method according to claim 1,
Wherein the horse riding extract is formed by one or more methods selected from the group consisting of hot water extraction, alcohol extraction and alcohol extraction
A composition for congenital immune enhancement and antiviral use.
The method according to claim 1,
Characterized in that it further comprises a pharmaceutically acceptable carrier, excipient or diluent
A composition for congenital immune enhancement and antiviral use.
The method according to claim 1,
Wherein the pharmaceutical composition is in a form selected from the group consisting of tablets, pills, powders, granules, capsules, suspensions, emulsions, syrups, aerosols, external preparations, suppositories and injections
A composition for congenital immune enhancement and antiviral use.
The method according to claim 1,
Bacterial infectious diseases or viral infectious diseases, in particular influenza, avian influenza, Newcastle virus, Vesicular stomatitis virus, Cossackievirus, Enterovirus-71, Herpes Simplex Virus ), Foot and mouth disease (FMD), Colorado spot mite fever virus, reovirus, human immunodeficiency virus, and hepatitis B virus type C, swine fever virus, bovine viral diarrhea virus, Wherein the virus is used for the prophylaxis or treatment of a porcine reproductive and respiratory syndrome virus, porcine Ozski disease, rotavirus, parvovirus, porcine epidemic diarrhea virus and the like
A composition for congenital immune enhancement and antiviral use.
Congenital immune enhancement and antiviral health functional food including horse riding extract.
The method according to claim 6,
Wherein the horse riding extract is formed by one or more methods selected from the group consisting of hot water extraction, alcohol extraction and alcohol extraction
Congenital immunity enhancement and antiviral health functional foods.
The method according to claim 6,
Characterized in that it further comprises a food-acceptable food-aid additive
Congenital immunity enhancement and antiviral health functional foods.
The method according to claim 6,
Wherein the health functional food is a form selected from the group consisting of powder, granule, tablet, capsule, candy, chewing gum, jelly and beverage
Congenital immunity enhancement and antiviral health functional foods.
A composition for congenital immunity enhancement and antiviral feed comprising horse riding extract. 11. The method of claim 10,
Wherein the horse riding extract is formed by one or more methods selected from the group consisting of hot water extraction, alcohol extraction and alcohol extraction
Composition for congenital immunity enhancement and antiviral composition.
A method for enhancing innate immunity and antiviral activity in an animal, which comprises administering an equine horse extract to an animal in need of innate immune enhancement other than human.
13. The method of claim 12,
Wherein the horse riding extract is formed by one or more methods selected from the group consisting of hot water extraction, alcohol extraction and alcohol extraction
Methods for enhancing innate immunity and antiviral activity in animals.

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