CN101856415B - Chinese herbal medicament oral liquid for treating chicken respiratory infectious disease - Google Patents
Chinese herbal medicament oral liquid for treating chicken respiratory infectious disease Download PDFInfo
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Abstract
The invention relates to Chinese herbal medicament oral liquid for treating chicken respiratory infectious disease, which is a preparation prepared from the following raw materials in part by weight: Cyrtomium fortunei 4-6, ephedra herb 4-6, tangerine peel 4-6, wild buckwheat rhizome 2-4, spreading hedyotis herb 2-4, platycodon root 2-4, common selfheal fruit-spike 2-4, astragalus root 1-3, baical skullcap root 1-3, Indian buead 1-3, manchurian wildginger 0.1-0.3, and liquoric root 0.5-1.5. When used for treating chicken respiratory infectious disease, the Chinese herbal medicament oral liquid has the characteristics of short treatment period, high cure rate, safety, no toxic nor side effects and convenient administration.
Description
Technical field
The present invention relates to the to raise chickens Chinese herbal and crude drugs preparations of usefulness is specifically related to a kind of Chinese herbal medicine oral liquid for the treatment of the chicken respiratory infectious disease.
Background technology
The chicken respiratory infectious disease is a class disease that extensively takes place in the modern poultry husbandry, with cough, get rid of that head, bronchorrhagia, larynx are hemorrhage, laying rate decline, death etc. are principal character, this disease is having a strong impact on growth promoter and the egg laying performance of chicken, causes very big loss to poultry husbandry.At present, the medicine of treatment chicken respiratory infectious disease is a lot, and adopting more is antibiotics class Western medicine, though have certain curative effect, but shortcoming is that Drug resistance and side effect are more serious, and it is residual to have chemicals in Carnis Gallus domesticus and egg, brings potential threat to human health; That Chinese herbal medicine has is natural, have no side effect, do not have residual, the characteristics such as be difficult for developing immunity to drugs of chemicals, be used for poultry husbandry diseases prevention, cure the disease, be with a wide range of applications.But the present commercially available Chinese herbal medicine that is used for the treatment of the chicken respiratory infectious disease, result of use is poor, and cure rate is low, and the course of treatment is long and cost is high.
Summary of the invention
The objective of the invention is at the problems referred to above, a kind of Chinese herbal medicine oral liquid that can effectively treat the chicken respiratory infectious disease is provided.
Technical scheme of the present invention is: a kind of Chinese herbal medicine oral liquid for the treatment of the chicken respiratory infectious disease, and it is made by following raw materials by weight proportions: Rhizoma Osmundae 4-6 part, Herba Ephedrae 4-6 part, Pericarpium Citri Reticulatae 4-6 part, Rhizoma Fagopyri Dibotryis 2-4 part, Herba Hedyotidis Diffusae 2-4 part, Radix Platycodonis 2-4 part, Spica Prunellae 2-4 part, Radix Astragali 1-3 part, Radix Scutellariae 1-3 part, Poria 1-3 part, Herba Asari 0.1-0.3 part, Radix Glycyrrhizae 0.5-1.5 part.
The preferred feedstock medicine weight proportion of making Chinese herbal medicine oral liquid of the present invention is: 5 parts of Rhizoma Osmundae, 5 parts in Herba Ephedrae, 5 parts of Pericarpium Citri Reticulataes, 3 parts of Rhizoma Fagopyri Dibotryiss, 3 parts of Herba Hedyotidis Diffusaes, 3 parts of Radix Platycodoniss, 3 parts of Spica Prunellaes, 2 parts of the Radixs Astragali, 2 parts of Radix Scutellariaes, 2 parts in Poria, 0.2 part of Herba Asari, 1 part in Radix Glycyrrhizae.
Each component pharmacological effect is in the prescription of the present invention:
1, Herba Ephedrae effect: Herba Ephedrae mainly contains alkaloid, volatile oil and flavone, organic acid, tannin, pectin, cellulose etc.Experiment in vitro proves, Herba Ephedrae volatile oil all has in various degree inhibitory action to staphylococcus aureus, first type and beta hemolytic streptococcus, hemophilus influenza, Diplococcus pneumoniae, diphtheria corynebacterium, escherichia coli, neisser's diplococcus etc., Influenza virus A-prime there is obvious inhibitory action, the mice that infects influenza A virus PR8 strain is had therapeutical effect.Zoopery proves that the Herba Ephedrae water extract has antitussive, phlegm-dispelling functions.
2, Pericarpium Citri Reticulatae effect: Pericarpium Citri Reticulatae mainly contains volatile oil, hesperidin, neohesperidin, naringenin, dihydro Nobiletin etc.The contained volatile oil of Pericarpium Citri Reticulatae has the zest phlegm-dispelling functions, makes the easy expectoration of sputum; Bright Pericarpium Citri Reticulatae decoct has dilating effect preferably to laboratory animal rabbit bronchus; The Pericarpium Citri Reticulatae alcohol extract has than the effect of prednisone relaxation guinea-pig isolated trachea, Pericarpium Citri Reticulatae alcohol extract 0.02g (crude drug)/ml contraction of guinea-pig isolated trachea due to the antagonism histamine fully; Hesperidins etc. have the effect of Citrin sample, can reduce capillary fragility, prevent microvascular bleeding.In addition, Pericarpium Citri Reticulatae still has effects such as immunomodulating, antiinflammatory.
3, Rhizoma Fagopyri Dibotryis effect: the Rhizoma Fagopyri Dibotryis rhizome mainly contains coumaric acid, ferulic acid; Herb mainly contains quercitrin, Quercetin, globulariacitrin.Rhizoma Fagopyri Dibotryis have eliminate the phlegm, effect such as analgesic, antiinflammatory, antitumor, coagulase, hemotoxin and the bacillus pyocyaneus endogenous toxin of staphylococcus aureus have antagonism.
4, Herba Hedyotidis Diffusae effect: Herba Hedyotidis Diffusae mainly contains hentriacontane, stigmasterol, maloic acid, oleanolic acid, flavonoid glycoside and Bulbus Lilii Herba Hedyotidis Diffusae element etc.Vitro antibacterial activity is not obvious, only staphylococcus aureus and dysentery bacterium is had faint inhibitory action, and high concentration water's decoct can suppress the growth of bacillus pyocyaneus, Bacillus typhi, Bacillus proteus.Its crude extract aqueous solution can strengthen laboratory animal Mus, rabbit and people's leukocytic phagocytosis.Herba Hedyotidis Diffusae is remarkable to the experimental typhlitic therapeutic effect of laboratory animal rabbit, and body temperature decline, inflammation are disappeared substantially.
5, Radix Platycodonis effect: Radix Platycodonis mainly contains polysaccharid glucoside, polysaccharide, steroidal and glucoside thereof, fatty oil, the fatty acid of five rings terpenoid etc., is used for that heat-phlegm is astringed the lung and the cough that rises, has lung qi dispersing to eliminate the phlegm, effects such as evacuation of pus detumescence.After gavaging Radix Platycodonis decoct 1g/kg body weight after the anesthesia of laboratory animal dog, can significantly increase the mucous secretory volume of respiratory tract, its intensity is similar to ammonium chloride, and postanesthetic cat is also had tangible phlegm-dispelling functions, and is sustainable more than 7 hours; Platycodin can also obviously suppress the permeability of anaphylactic shock mice blood capillary; The no direct antibacterial action of Radix Platycodonis itself, but its water extract can strengthen the phagocytic function of macrophage, strengthens the sterilizing power of neutrophilic leukocyte, improves activity of lysozyme; Heating mice due to typhoid fever, the paratyphoid fever vaccine all there is significant refrigeration function.
6, Spica Prunellae effect: herb contains triterpenoid saponin, oleanolic acid, ursolic acid, globulariacitrin, hyperin, suitable-caffeic acid, anti--caffeic acid, vitamin B
1, vitamin C, vitamin K, carotene, resin, amaroid, tannin, volatile oil, alkaloid, water soluble salt etc.The water-leach liquor of Spica Prunellae, alcohol-water leachate and 30% alcohol leaching liquid are to the anesthetized animal effect of bringing high blood pressure down.The Spica Prunellae decoct has inhibitory action to dysentery bacterium, Bacillus typhi, vibrio cholera, escherichia coli, Bacillus proteus, bacillus pyocyaneus and staphylococcus, streptococcus, antimicrobial spectrum is also wider, and its water logging agent (1: 4) also has inhibitory action to some common pathogenic dermatophytes in vitro.
7, Rhizoma Osmundae effect: Rhizoma Osmundae mainly contains Filixic Acids, flavaspidic acid, albaspidin, dryocrassin, the α-octose-δ of D-Portugal-lactone-enediol, iso-amylene gland glycoside, wild goose tooth alkene etc., has heat-clearing and toxic substances removing, the effect of parasite killing dissipating blood stasis; But Embryo Gallus domesticus test strong inhibition influenza virus also has stronger antivirus action to adenovirus, poliovirus and encephalitis b virus, and certain antibacterial action is arranged in addition; Decoct and refining after effective ingredient to the laboratory animal rabbit exsomatize and there is significant excitation in uterus on the throne, shrink to strengthen, tension force improves.
8, Radix Scutellariae effect: Radix Scutellariae mainly contains baicalin, baicalin, wogonoside, wogonin, neobaicalein I, neobaicalein II, nor-wogonin, 7-methoxyl group baicalin, 7-methoxyl group demethyl wogonin etc., also have volatile oil, phenethanol glucosides constituents etc. in addition, has heat clearing and damp drying, eliminating fire and detoxication, hemostasis, antiabortive effect.Radix Scutellariae has antibacterial action, and the external treadmill test of Radix Scutellariae decoct has inhibitory action to dysentery bacterium, Bacillus typhi, Salmonella paratyphi, escherichia coli, Bacillus proteus, bacillus pyocyaneus, staphylococcus, Hemolytic streptococcus, Diplococcus pneumoniae; Other has antivirus action.
9, Radix Astragali effect: the Radix Astragali contains various trace elements such as Saponin, flavone, polysaccharide, several amino acids, folic acid and selenium, zinc, copper, has the enhancing human body immunity function, protects the liver, diuresis, blood pressure lowering, defying age, anti-hypoxia, radioprotective, anti-stress, low temperature resistant and high temperature resistant effect.Animal experiment proves, can increase the phagocytic function of reticuloendothelial system, and enhancing antibody forms, and improves E-rosettes rate, and the differentiation that promotes the T cell strengthens the cytotoxic activity of NK cell with ripe, induces the generation interferon; The inductive chicken peripheral blood B of bacteria lipopolysaccharide lymphocyte in-vitro multiplication also there is obvious facilitation; The effect of strengthening capillary resistance is arranged, the phenomenon of the increased capillary permeability that can cause antihistaminic, chloroform.The Radix Astragali has stronger antibacterial action external to dysentery bacterium, diphtheria corynebacterium, Hemolytic streptococcus, Diplococcus pneumoniae, staphylococcus aureus etc.The Radix Astragali has inhibitory action to the cytopathy due to animal influenza virus, the adenovirus etc.
10, Poria effect: Poria mainly contains β-pachyman, and other contains chemical constituents such as triterpenes pachymic acid, su-fuling.Pachyman is the enhancing human body immunity function significantly, increases the weight of immune organ thymus, spleen, lymph node; Strengthen the normal mouse phagocytosis of macrophages; Improve the body specific immune function, improve Flos Rosae Rugosae formation rate and lymhocyte transformation rate; Su-fuling has regulating action to immunologic function.In addition, Poria also have antibiotic, promote pharmacological actions such as hemopoietic function, calmness.Animal experiment has diuresis, calmness and hypoglycemic activity, can suppress staphylococcus aureus, escherichia coli, leptospira growth.
11, Herba Asari effect: Herba Asari mainly contains methyleugenol, safrole, eucarvone, nopinene, australene, elemicin, asaricin, myrcene, Borneolum Syntheticum, α-thujene, myrcene, γ-terpineol, alpha-terpineol etc., has expelling wind and cold, understand things pain-stopping, effects such as warming the lung to resolve fluid-retention.Herba Asari volatile oil has tangible central inhibitory action, and low dose can make animal peace and quiet, taming, autonomic activities minimizing; Heavy dose can make animal sleep, and tangible anticonvulsant action is arranged, and the Herba Asari decoct has gavaged analgesic activity.Herba Asari volatile oil gavages has tangible refrigeration function to multiple reason such as tetrahydrochysene beta-naphthylamine, typhoid fever, the heat pyrexia of the caused laboratory animal rabbit experiment of paratyphoid fever mixed vaccine, the rat fever due to the beer yeast is also had significantly separate thermal effect; Gavage or inject tangible antiinflammatory action is all arranged.The animal isolated experiment proves that Herba Asari volatile oil has tangible antagonism to histamine and the caused bronchospasm of acetylcholine; Kakuol preserved material, volatilization wet goods have certain vitro inhibition effect to gram positive bacteria, bacillus subtilis and Bacillus typhi, and decoct also has inhibitory action to tubercule bacillus and Bacillus typhi.Herba Asari volatile oil all has inhibitory action to multiple fungus such as Aspergillus flavus, Aspergillus niger, Candida albicans etc.α-asaricin has the effect that suppresses respiratory syncytial virus propagation.
12, Radix Glycyrrhizae effect: Radix Glycyrrhizae mainly contains compositions such as glycyrrhizic acid, glycyrrhizin, 24-hydroxyl enoxolone, licoflavone, glycyrrhizin, isoliquiritigenin, Angelica Polysaccharide, has antiulcer, promotes pancreatic secretion, protects the liver, the effects such as phagocytic function of blood fat reducing, adjusting macrophage.Glycyrrhizic acid is natural antidote, can be used for body defying age, enhancing immunity, keeps water-electrolyte metabolism balance etc.; Angelica Polysaccharide has anti-adenovirus type III, herpes simplex virus I-type activity.
Prescription foundation of the present invention: the prdc of chicken, after respiratory tract infection antibacterial, virus, mycoplasma etc., accumulate in the pyretic toxicity, phlegm retention is blocked trachea, bronchus, cause cough, asthma, hello sound, nasal cavity has nose liquid etc., so should be with removing heat from the lung and dissipating phlegm in the treatment, eliminating carbuncle antiinflammatory, heat-clearing and toxic substances removing are the rule of treatment.Relieving asthma with Herba Ephedrae, Pericarpium Citri Reticulatae, Rhizoma Fagopyri Dibotryis, Herba Hedyotidis Diffusae, the swelling of Spica Prunellae alleviation trachea, warming the lung to relieve cough in the side, reducing phlegm looses becomes silted up clearing throat, gas falls along expectorant then, gasifies then that expectorant disappears, again because of expectorant by humidogene, spleen rehabilitation fortune is then wet can be changed, and wetly goes then that expectorant disappears, so add the Poria invigorating spleen to remove dampness for helping; Rhizoma Osmundae, Herba Ephedrae, Herba Hedyotidis Diffusae have heat-clearing and toxic substances removing, anti-inflammation, dispelling exopathogens from superficies of the body effect; Rhizoma Fagopyri Dibotryis has the effect of pair anti-mycoplasma; The Radix Astragali, Poria, Herba Hedyotidis Diffusae, Radix Glycyrrhizae can also be regulated body's immunity, and with sweet temperature QI invigorating of Radix Glycyrrhizae and middle spleen invigorating, current twelve channels and collaterals, coordinating the actions of various ingredients in a prescription.In the side all medicines mutually and, play heat-clearing and toxic substances removing, anti-inflammation, depressed lung-energy dispersing, drying dampness to eliminate phlegm altogether, the effect of human body immunity improving power and resistance against diseases.Be applicable to the chicken respiratory infectious disease due to antibacterial, virus, the mycoplasma.For convenient application in the fowl disease control, make oral liquid.
The present invention treats the Chinese herbal medicine oral liquid of chicken respiratory infectious disease, be on the basis of having carried out a large amount of practical applications, according to the traditional medicine ancient prescription, form in conjunction with sick of modern popular, nosetiology and immunologic theoretical screening, treatment chicken respiratory infectious disease is had excellent curative.
The specific embodiment
The invention will be further described below in conjunction with embodiment.
Embodiment:
1, takes by weighing raw material (decoction pieces): Rhizoma Osmundae 146g, Herba Ephedrae 146g, Pericarpium Citri Reticulatae 146g, Rhizoma Fagopyri Dibotryis 88g, Herba Hedyotidis Diffusae 88g, Radix Platycodonis 88g, Spica Prunellae 88g, Radix Astragali 58g, Radix Scutellariae 58g, Poria 58g, Herba Asari 6g, Radix Glycyrrhizae 30g respectively;
2, the above-mentioned raw materials pulverize separately is become the Chinese herbal medicine coarse powder;
3, get Pericarpium Citri Reticulatae coarse powder 146g, Herba Asari coarse powder 6g, put in the distil container, use steam distillation, heating extraction volatile oil is collected the volatile oil that distills out, and puts in the container and preserves; Other gets Rhizoma Osmundae 146g, Herba Ephedrae 146g, Rhizoma Fagopyri Dibotryis 88g, Herba Hedyotidis Diffusae 88g, Radix Platycodonis 88g, Spica Prunellae 88g, Radix Scutellariae 58g, Radix Astragali 58g, Poria 58g, Radix Glycyrrhizae 30g ten flavor Chinese herbal medicine coarse powder, adding water 7000ml soaked after 1 hour, medicinal residues after above-mentioned distillation, water are opened back slow fire boiling 30 minutes; Filter, add water 5000ml more at every turn and decoct secondary, each 20 minutes, filter; Merge three times filtrate, be concentrated into about 900ml;
4, volatile oil that above-mentioned distillation is obtained and concentrated solution merge, and add 0.05% sodium benzoate as antiseptic, add water to 1000ml, stir evenly, filter, and fill, sterilization promptly gets Chinese herbal medicine oral liquid of the present invention.
Some test situation of utilizing the present invention to carry out are as follows:
1, artificial challenge's infectious laryngotracheitis of chicken therapeutic test
200 the 1 blue brown chickens in age in days sea immune newcastle disease vaccine of difference when 7 ages in days, 27 ages in days, immunity fabricius bursa trivalent vaccine when 12 ages in days, 22 ages in days, not immune infectious laryngotracheitis of chicken vaccine, father and mother are for no infectious laryngotracheitis history.When 43 ages in days, be divided into 5 groups at random, 40 every group, totally 200, counteracting toxic substances during 50 ages in days, every chicken is from tracheal instillation chick embryo allantoic liquid (ILTV) 0.2ml (ID5010
4.2/ 0.2ml), the successive administration time is 7d.Test grouping and handle and seeing Table 1, prevention effect sees Table 2, and the chicken increase heavy influence of artificial challenge's infectious laryngotracheitis of chicken is seen Table 3.
Table 1 test grouping and processing (unit: only)
Table 2 Chinese herbal medicine oral liquid of the present invention is to the prevention effect of artificial challenge ILT (unit: %, only)
Annotate: a represents and C
1Group significant difference (P＜0.05), aa are represented difference extremely significantly (P＜0.01); B represents A
1, B
1With B
2Group significant difference (P＜0.05), bb are represented difference extremely significantly (P＜0.01); Dd represents difference extremely significantly (P＜0.01).
3d behind counteracting toxic substances does not prevent the indivedual chicken morbidities of administration group, cough, snore is arranged, get rid of first-class symptom.The sick appearance of 4d chicken mass-sending behind counteracting toxic substances is the highest, especially B group and C
1Group is all morbidities almost, all are reduction trend then, and at 4d, by comparing sickness rate, treatment group A and treatment group B and C group have utmost point significant difference (P＜0.01), and the experimental observation phase finishes by respectively organizing cure rate each group and C
1Organize poor heteropole significantly (P＜0.01), A, B
1With B
2Group significant difference (P＜0.05), wherein counteracting toxic substances matched group 4 chickens in the 4th～6d are dead because of hemoptysis, A, B
1There is not the disease symptom chicken at 5d, 7d, 9d respectively.
Table 3 Chinese herbal medicine oral liquid of the present invention is to the influence (unit: g) of artificial infection chicken's weightening finish
Annotate: aa represents and and C
1Group difference is (P＜0.01) extremely significantly
As can be seen from Table 3, the present invention has in various degree effect of gain to artificial challenge's chicken infectious laryngotracheitis chicken, each administration group and counteracting toxic substances matched group relatively, difference is (P＜0.01) extremely significantly, and A, B
1, B
2, C
2Difference also extremely significantly (P＜0.01) between each group.
2, natural infection infectious laryngotracheitis of chicken therapeutic test
Grow up laying hen, chickling of natural occurrence provided by Changli County, Hebei province, Funing County, Lulong County, each raiser of Leting County respectively, is infectious laryngotracheitis of chicken according to epidemiology, clinical symptoms, pathological change, inclusion body inspection and agar diffusion test clinical diagnosis.With chicken infected separately be test group, Chinese herbal medicine oral liquid of the present invention mixed drink in water by only the grow up dosage of laying hen of 1ml/ chickling, 2ml/ according to morbidity chicken group, every day 2 times, dispensing 5～7d is a course of treatment.Continue to observe 3～5d after the drug withdrawal, with clinical symptom disappearance, drink water, search for food and recover normal person and be judged to healing, binding of pathological cuts open inspection and takes a sample test the result and determine prevention effect, the statistics cure rate again.Therapeutic outcome sees Table 4, table 5.
Therapeutic effect (the unit: %) of the adult laying hen of table 4 pair natural occurrence
The therapeutic effect of table 5 pair natural occurrence chickling (unit: only, %)
Treatment by laying hen that 5500 natural infections are grown up, Chinese herbal medicine oral liquid of the present invention has notable therapeutic effect to the infectious laryngotracheitis of the laying hen that grows up, cure rate is more than 98%, generally through 5～7d transference cure, recover normally to search for food, laying rate is gone up, through returning in 2～4 weeks near the level of normally laying eggs.By 6100 different days chickling treatments are observed, Chinese herbal medicine oral liquid of the present invention has significant therapeutic efficiency to the infection laryngotracheitis of chickling, and cure rate is more than 97%, and generally through the sick young rehabilitation of 5～7d, body weight has in various degree to be increased.Dispensing whole process does not see that the chicken group has any untoward reaction, and the chicken dissection is cured in selective examination, and trachea, larynx there is no observes pathological change, and histological examination larynx, trachea etc. are normal substantially.
Claims (2)
1. a Chinese herbal medicine oral liquid for the treatment of the chicken respiratory infectious disease is characterized in that it is the medicament of being made by following raw materials by weight proportions: Rhizoma Osmundae 4-6 part, Herba Ephedrae 4-6 part, Pericarpium Citri Reticulatae 4-6 part, Rhizoma Fagopyri Dibotryis 2-4 part, Herba Hedyotidis Diffusae 2-4 part, Radix Platycodonis 2-4 part, Spica Prunellae 2-4 part, Radix Astragali 1-3 part, Radix Scutellariae 1-3 part, Poria 1-3 part, Herba Asari 0.1-0.3 part, Radix Glycyrrhizae 0.5-1.5 part.
2. the Chinese herbal medicine oral liquid of treatment chicken respiratory infectious disease according to claim 1 is characterized in that it is the medicament of being made by following raw materials by weight proportions: 5 parts of Rhizoma Osmundae, 5 parts in Herba Ephedrae, 5 parts of Pericarpium Citri Reticulataes, 3 parts of Rhizoma Fagopyri Dibotryiss, 3 parts of Herba Hedyotidis Diffusaes, 3 parts of Radix Platycodoniss, 3 parts of Spica Prunellaes, 2 parts of the Radixs Astragali, 2 parts of Radix Scutellariaes, 2 parts in Poria, 0.2 part of Herba Asari, 1 part in Radix Glycyrrhizae.
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| CN104491081A (en) * | 2014-12-19 | 2015-04-08 | 河北科技师范学院 | Traditional Chinese herbal medicine oral liquid for treating chicken viral respiratory infectious disease |
| CN105748656A (en) * | 2016-03-02 | 2016-07-13 | 江西傲新生物科技有限公司 | Medicine preparation for preventing and treating bacterial respiratory diseases of livestock and poultry and preparation method thereof |
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