CN101411369A - Rhodiola rosea cold tea - Google Patents

Abstract

The invention belongs to the technical field of functional drink, in particular to functional drink which is prepared by the advanced technology after adoption of Tibet rhodiola, Tibet Matrimony vine, Tibet seabuckthorn, Tibet Potentilla anserine, honeysuckle, Tibet liquorice, green tea and so on as raw materials. The preparing technology for the product is as follows: mixing and crushing of the raw materials, secondary leaching, preparing and mixing, evenly stirring, filtering, filling and capping, sterilizing, inspecting and packaging, so as to obtain the finished product. The product contains the raw materials such as the Tibet rhodiola, the Tibet Matrimony vine, the Tibet seabuckthorn, the Tibet Potentilla anserine, the honeysuckle, the Tibet liquorice, the green tea and so on, and has the efficacies of resisting fatigue and anoxia, reinforcing immunity and so on.

Description

Rhodiola rosea cold tea

One, technical field: the invention belongs to drinks, especially with raw materials such as Qinghai-Tibet Platean free of contamination wild plant safranine red-spotted stonecrop, Tibetan matrimony vine, Tibetan sea-buckthorn, Tibetan fern fiber crops, Tibetan Radix Glycyrrhizae, honeysuckle, green tea, the drinks of taking advanced technologies to process.

Two, background technology: this is invented with the safranine red-spotted stonecrop, hides matrimony vine, hides sea-buckthorn, hides the fern fiber crops, hides the drinks that raw materials such as Radix Glycyrrhizae, honeysuckle, green tea are made, and this beverage has antifatigue, anti-anoxic, strengthens the immunity effect.Also do not have the appearance of this series products in the market, develop this product and can be a kind of new function product to beverage industry is provided.Soft, the good taste of this product mouthfeel is the good merchantable brand in the beverage.

Three, summary of the invention: along with the constant development of economy of China, improving constantly of national economy income and living standard, huge variation has taken place in people's consumption idea, particularly free of contamination plant beverage more is subjected to consumer's welcome to beverage consumption with the speed increment more than 20 percent every year.Development of the West Regions, being open to traffic of Qinghai-Tibet Railway attracted a large amount of domestic and international investment entrepreneurs and other staff and a large amount of visitors to come beautiful Qinghai-Tibet sightseeing tour both at home and abroad, bring huge business opportunity for Qinghai-Tibet Wild plant beverage, have good market development prospect.

Four, composition of raw materials: raw materials such as safranine red-spotted stonecrop 6-8 kilogram, Tibetan matrimony vine 4-6 kilogram, Tibetan sea-buckthorn 4-6 kilogram, Tibetan fern fiber crops 8-10 kilogram, Tibetan Radix Glycyrrhizae 2-3 kilogram, honeysuckle 6-8 kilogram, green tea 2-4 kilogram, rock sugar 40-60 kilogram.

Five, technical scheme:

(1), Tibetan medicine rhodiola root (HONgingtian), the Tibetan medicine rhodiola root be grown in the high and cold anoxic in 3500 meters-5000m Qinghai-Tibet Plateans, drying, ultraviolet ray is strong, day and night temperature is big, the wild plant of extreme natural environment.The Tibetan medicine rhodiola is in the herb of Crassulaceae plant rhodiola root, and is cold in nature, flavor is sweet, puckery, has effects such as promoting blood circulation and removing blood stasis, removing heat from the lung to relieve cough.Tibetan medicine's " brilliant strain book on Chinese herbal medicine " puts down in writing it foster lung, heat-clearing, tonifying primordial Qi, go effects such as halitosis.Tibetan is referred to as " plateau genseng ".That modern medicine study is widely used in is anti-ageing, regulate immunologic function, cardiac stimulant antifatigue, anti-anoxic, radioresistance, raising is mental and specific functions such as physical function, anti-peroxidation.As treat high altitude anoxia and HAHD etc.Tibetan are used for the treatment of spitting of blood, haematemesis, pneumonia, cough and women's diseases such as leukorrhea often.In recent years China discovers, and is the rarest in the known tonic of the pharmacological action of safranine red-spotted stonecrop.Immunization is better than genseng, and without any side effects.The seventies in 20th century, the former Soviet Union studies have shown that, rhodiola root have the similar traditional Chinese medical science " strengthen the body resistance to consolidate the constitution " have " adaptation former state " effect.According to foreign literature, clinical practice shows that sachalin rhodiola rhizome has moist strong, and old heart failure and impotence are had therapeutic action, improves muscle power and mental labour function.The powder of rhizome or medicinal extract are used for diabetes, pulmonary tuberculosis and anaemia, and neuropathy and low blood pressure are also had effect.

Chemical analysis: mainly contain rhodioloside and Jujubogenin tyrosol, protein, fat, 19 seed amino acids, alkaloid, flavone compound, phenolic compound, bioactivity trace element, compositions such as iron, zinc, cobalt, copper, manganese.

Pharmacology is done: 1, regulate immunity: China discovers in recent years, and the pharmacological action of rhodiola root is rare, and immune strong function is better than genseng.Rhodiola root can be transferred the human body self immunologic function, and the interior environment of human body imbalance is corrected, and qi and blood is able to unimpeded, thus the natural anti-ability of unloading of exciting human, the slack state of change organ.

2, regulate cardiac function, anti-cardiac muscle aging: rhodioloside and ketone all can significantly reduce the rat left ventricular end diastolic at short notice and press (LYEDP) and femoral artery mean pressure (BP).Illustrate that rhodioloside and ketone all can reduce heart front and back load, change cardiac function.The rhodiola root decoction can make the isolated frog heart shrinkage amplitude increase by 30%, gives at the subcutaneous lymph injection of body frog rhodioloside, can make the heart contraction amplitude increase by 128.6%.Rhodiola essence can obviously increase the SOD in rat blood serum and the cardiac muscle, obviously reduces LPO, and rat heart muscle superstructure aging phenomenon is obviously alleviated, and prevention is arranged and delay the old and feeble effect of cardiac muscle.According to another external report, rhodiola root has the strengthening by means of tonics effect, and old heart failure is had smelting treatment effect.

3, anti-arrhythmia: the rhodioloside rat rhythm of the heart that can slow down, have stronger anti-oxidant barium and bring out the rat ventricular effect, the tachycardia of its generation there is stronger role of correcting.

4, protection cardiac muscle: rhodioloside can reduce mouse under the situation of severe depletion of oxygen; the lactic acid content of brain and cardiac muscle; cardiac muscle CAMP/cyclic guanosine monophosphate ratio when recovering anoxic has protection heart muscle capillary endothelial cell, the effect that alleviates injury of mitochondria.Compositions such as rhodiola root general glycoside can protect rat, neonatal rat myocardial cell to cultivate scarce sugared hypoxic damage, reduce the release of lactic dehydrogenase when giving oxygen injury again.

5, anti-oxidant: rhodioloside can show the concentration that reduces the liver Serum LPO, has stronger anti-finger matter peroxide and turns into.Rhodiola root decoction and tyrosol can suppress rat liver homogenate endoperoxides lipid at experiment in vitro and generate.Tyrosol also has inhibitory action to rat brain and the generation of heart homogenate endoperoxides lipid.Can suppress mice serum endoperoxides lipid to the mouse multiple dosing and generate, the generation of the peroxide in mouse brain, the heart and the liver is also had the obvious suppression effect.

6, anti-ageing: rhodiola essence can prolong going down to posterity the life-span of 2BS cell, promotes growth and proliferation of cell, strengthens liver Adenosine Triphosphate enzyme (ATP-ase) and reduce acid phosphatase (activity of ACO-ase) promotes DNA synthetic, improves macrophage phagocytic function.

7, antifatigue: the rhodiola root decoction can the swimming time of significant prolongation mouse in frozen water.Gadol extract and rhodioloside can prolong the mouse swimming with a load attached to the body time.Gadol extract can prolong mouse and grab rod to the tired time, and the mouse subcutaneous injection rhodioloside also can strengthen the mouse anti-reflecting fatigue function.

8, anti-anoxic: the anti-anoxic experiment of mouse shows that oral rhodiola root alcohol medicinal extract (RKEE) can improve the endurance of brain tissue to anoxic, prolongs the time-to-live, and the cerebral anoxia process is had the improvement effect, can strengthen the oxygen tension of brain tissue.

9, high temperature resistance: mouse is irritated stomach rhodiola root decocting liquid 10g/kg every day, can obviously improve the mouse anti high temperature capabilities.

10, promoting blood circulation and removing blood stasis: as to it is reported: utilize double-blind study that rhodiola root treatment plateau cytosis is carried out observation of curative effect, the result shows that rhodiola root has certain function of promoting blood circulation to disperse blood clots.

11, regulate endocrine: rhodiola root can excite nerve system and bring into play endocrine regulating action.Studies show that rhodiola root has extremely strong regulating action to endocrine system, especially internal system is had tangible opsonic action, women's gynecological disease is had fine booster action.Mechanism is to strengthen thyroid gland, adrenal gland skin etc. and ovarian function.

12, improve muscle power and activation central nervous system: modern study finds that rhodiola root has crucial meaning as several environmental adaptation medicines in military affairs, medical science, aerospace medicine.Can be used as all and be in the good medicine that the staff in the undesired environment builds up resistance, it all has tangible double-bang firecracker regulating action to improving muscle power and mental labour efficient, is desirable tonic.It is reported that foreign study finds that rhodiola root can activate nerve and antifatigue.Behind the oral rhodiola root of the former person of will, memory is improved in a short time, notice is concentrated, the tired improvement, shows that rhodiola root truly has brain tonic, improves the effect of memory.Not it is reported that the former Soviet Union is used for astronaut's health care and antisecosis to the mysterious rhodiola root of natural plant of northwest Leah extremely frigid zones, its effect is fairly obvious.1976 with rhodiola root with being to adapt to the former state medicine, be widely used in astronaut, pilot, diver etc.It is also regarded as wuwa by kremlin.

13, improve sleep: the rhodiola root decoction can obviously strengthen the pentothal syngignoscism, the convulsions effect of antagonism caffeine sodium benzoate.Rhodioloside can significantly shorten the sagatal time for falling asleep and prolong its length of one's sleep.

14, dual regulation: remote measurement animal brain electric wave is observed and is shown, use rhodiola root can make drug-induced excited type or inhibition type brain wave recover normal, environmental factor makes the organism physiology variation no matter to that aspect development, uses rhodiola root can make it recover normal level.

15, anti-inflammatory: can significantly suppress rat paw edema due to the dextrorotation powder liver for rats by intraperitoneal injection rhodiola root water decoction 0.5,1.0/kg.Hypodermic injection rhodioloside 10mg/kg, the leukocytosis that can prevent hypodermic injection turpentine oil to cause.

16, antitoxin: rhodiola root can improve the survival rate after mouse poisons, and the antagonism tetanus toxoid waits various bacterial disease elements, improves the immunological effect of brucella vaccine.

17, antiviral: the sachalin rhodiola rhizome polysaccharide can stop the suction-operated of virus at host cell effectively, and the reproduction process in host cell has inhibitory action to virus simultaneously.The human amniotic cell that sachalin rhodiola rhizome tyrosol (RABT) add to be cultivated to the attack of being afraid of Sa Qi B5 virus have certain resistant function in, mechanism may be RABT and virus some acceptor site on the competition cell film simultaneously, thereby stops or disturbed viral absorption or link such as penetrated.

18, promote ATP synthetic: rhodiola root medicinal extract can improve the rna content of skeletal muscle, promotes ATP synthetic again, improves the muscle power and the mental labour efficient of human body.Rhodiola root also has hypotensive and hypoglycemic activity in addition.

(2), hide sea-buckthorn (Seahuckth.Rn), be Qinghai-Tibet a kind of wild plant---the sea-buckthorn of growth more than 3000 meters.Because Qinghai-Tibet Special geography ecological environment and weather conditions, high and cold anoxic, sunshine-duration are long, and day and night temperature is big, bred to have special bioactive substance content and enrich, and be the top grade of other various places sea-buckthorn.Sea-buckthorn looks for " angle fruit " in the Tibetan that lives in the Qinghai-Tibet Platean, and extremely strong medical value is arranged.At Tibetan medicine's monumental work Four-Volume Medical Code and " Qinghai-Tibet medicine history " argumentation is arranged all.It is the medicine food dual purpose plant that the Ministry of Public Health announces.

Modern medicine research is thought, the Tibetan medicine sea-buckthorn have build up health, regain one's strength, eliminate fatigue, strong spirit, patient myocardial ischemia anoxic, anti-ageing, anti-strong, hypotensive, prevent and treat artery sclerosis, cerebral thrombus has better prevention and pharmacological action, modern clinical to reduce cholesterol, to strengthen MBF, improve arrhythmia cordis etc.

Chemical composition: sea-buckthorn contains 14 kinds of vitamins, abundant vitamin C, E, A, B ₁, B ₂, folic acid, calcium, carrotene, flavone compound, alkaloid, 20 several amino acids, unrighted acid, monosaccharide, 12 kinds of organic acids, 32 kinds of trace elements, especially contain abundant superoxide dismutase more than 200 kind of bioactivators such as (SOD) to the human body beneficial.Be described as " Bao Ku of vitamin " by the expert of China and foreign countries.

Pharmacological action:

1, strengthen immunologic function: Seabuckthorn Oil and sea-buckthorn juice can strengthen immune organ weight, improve the serum half and hold blood value antibody titer and antigenic stimulus antibody titer once more.Promote macrophage phagocytic function.Similar sea-buckthorn juice of the effect of Hippophate flavone and Seabuckthorn Oil, and the mice serum antibody horizontal of immunologic hypofunction is raise.

2, antifatigue, anti-anoxic: evidence, total seabuckthorn flavone have the myocardial nutritional flow amount of increase, improve myocardium microcirculation, reduce MCO, have antifatigue, anti-anoxic, effects such as cold resistant and anti-sudden change.

3, anti-oxidant: hippophae rhamnoides can significantly reduce hyperlipemia and cultivate the LPO content that increases in the smooth muscle clearly, suppresses LPO rising in the mice serum of ozone damage back, improves the activity of superoxide dismutase.Total seabuckthorn flavone generates O to the PMA dysmorphocaryocyte ²---there is significant dosage to rely on scavenging action.The sea buckthorn juice gastric infusion can obviously strengthen normal tumor-bearing mice GSH-PX activity, reduces lipid peroxide contents.According to another report, total seabuckthorn flavone to the removing ability of active oxygen greater than vitamin E.Seabuckthorn Oil 2.5mg, mouse abdomen annotate or rat is annotated clothes, can obviously reduce the LPO of cardiac muscle etc., improve the SOD activity.

4, delay senility: sea-buckthorn can obviously prolong the life-span of wild oily flax fly.

5, antitumor: Hippophae (rhamnoides) original juice has certain inhibitory action to tumor cell proliferation in the mouse ascites; DNA in bone marrow cells in mice, people's acute myeloblastic leukemia cell, the mouse lymphocyte there is the obvious suppression effect; mechanism to the leukaemia cell growth inhibition may and suppress synergistic effect with the depolymerization that DNA is synthesized, and can watch for animals and avoid the damage of N-nitrosodimethylamine.

6, protection liver: studies show that Seabuckthorn Oil 2.25-4.50g/kg * 3d mouse gavaging or 0.84-1.68g/kg sea buckthorn juice can be protected CCL4, the hepatic injury due to the paracetamol.

7, resist myocardial ischemia and cardiac muscle not normal: the sea-buckthorn abdomen is annotated or quiet notes; mouse rat acute myocardial anoxia is had protective effect; make arrhythmia cordis reduce to 0 from 100%; total seabuckthorn flavone can increase the myocardial nutritional flow amount to mouse, rat, cavy; the antagonism ischemic arrhythmia; improve myocardial ischemia, promote cardiac function, angina pectoris efficient 94%.According to another report, hiding hippophae rhamnoides has and obviously resists myocardial ischemia and ARR effect.Evidence, total seabuckthorn flavone have the myocardial nutritional flow of increasing amount, improve myocardium microcirculation, reduce MCO, increase MBF, improve arrhythmia cordis.

8, prevent and treat heart disease: Tibetan its main drug ingedient of sea-buckthorn---Hippophate flavone has reducing blood lipid; serum lipids and liver lipid are obviously descended; increase the contraction and the diastolic function of heart; resist myocardial ischemia and effects such as anti-arrhythmia, anti-oxidant, coronary artery dilator, anti-coronary atherosclerosis, have the effect of protection heart.It is reported that the medical expert of U.S. Wei Kangsi star university discovers that flavonoids is to can be used as antioxidant, stop oxidation and low-density lipoprotein to bind (will be as binding, the overslaugh blood flow) attached on the vascular wall; The 2nd, as hematoblastic inhibitor, reduce hematoblastic stickiness.Test shows that the danger that the people that eats the abundant food of flavonoids for a long time suffers from a heart complaint reduces half.

9, reducing blood lipid: Seabuckthorn Oil, flavones can make T-CHOL in the hyperlipidemia rats serum, and the total cholesterol level in triglyceride levels decline and the hepatic tissue descends, and (DHL-C) content raises to make serum middle-high density albumen.

10, antithrombotic: Seabuckthorn Oil can make the rabbit experiment thrombosis postpone, and has prevention thrombosis and certain anticoagulation.Its effect is to participate in intrinsic coagulation system, obviously reduces fibrinogenic content, and this is relevant with fatty acids in the Seabuckthorn Oil.

11, to the effect of hematopoietic cell: Hippophae (rhamnoides) original juice has its growth effect of promotion to hematopoietic cell, and this effect may to be rich in vitamin C, E, A and each seed amino acid relevant with sea-buckthorn.

12, antiallergy: the Hippophate flavone lumbar injection can suppress the mouse passive cutaneous anaphylaxis, its anti-allergic effects link may not improved target organ pipe and cell effect, also play a role unlike medicines such as Piao Ermin competition receptor in target cell, may be relevant with factors such as combination that suppresses antigen or medium releases.

(3), hide matrimony vine (LyciUm barbarnm extraat), the fruit of Chinese wolfberry is the sub-ripening fruits of matrimony vine of solanaceae plant.The property flat, flavor is sweet.Have by the liver kidney, promoting vital essence and blood genernation makes eye bright, moistening lung, beauty treatment, functions such as fat-reducing.

Chemical composition: fruit of Chinese wolfberry main component has polysaccharide, amino acid, sweet theophylline, carrotene, vitamin B1, vitamin B2, various trace elements such as multivitamin such as vitamin C, nicotinic acid and manganese, chromium, zinc, copper, magnesium, calcium, germanium, cobalt, iron.

Pharmacological action: 1, protection birth-death system it is reported research of HSPH of Wuhan University

Achievement shows that LBP-X can produce protective effect to the reproductive system that is damaged by effect " hypothalamus-hypophysis-sexual gland " this axis.

Experiment shows that LBP-X promotes hypophysis secretion gonadal hormone by the gonadotropic hormone effect, and " hypothalamus-hypophysis-sexual gland " this axis is produced the multilayer regulating action, impels the production of sperm process unimpeded normal with the growth of testis reproduction cell.

2, improve sexual function: the fruit of Chinese wolfberry has filling liver kidney, promoting vital essence and blood genernation, supporing yang function.No matter be that kidney-yang deficiency, kidney-yin deficiency all have certain effect, Fructus lycii P.E also has the effect of short gonad function, and plasma testosterone level is significantly raise, and property kinetic energy is gone down, and impotence plays an important role.

3, strengthen immunity: the fruit of Chinese wolfberry is a medicine-food two-purpose food, and fruit of Chinese wolfberry polysaccharide has enhancing immunity, and antitumor action can strengthen the cell anti-cancer function selectively.LBP-X can strengthen natural killer cell and lymphocyte vigor, promotes interleukin to generate, and strengthens monocytic immune vigor.Fruit of Chinese wolfberry polysaccharide also has antimutagenic effect, when mutagenesis produces genetic damage and cell mutation, can repair damaged cell and recover its normal function.

4, anti-ageing: the fruit of Chinese wolfberry has the rejuvenation effect, and showing as to stimulate sexual gland and endocrine, strengthens hormonal secretion, strengthens the physiological function of brain function and nerve cell.The toxin phenomenon occurs accumulating in the blood though can avoid people's age growth, thereby keep the normal function of in-vivo tissue.But fruit of Chinese wolfberry polysaccharide antagonism free radical peroxidase activity strengthen superoxide dismutase and glutathione peroxidase vigor, thereby the damage that alleviates the radical pair body has anti-ageing effect.The plant of a kind of indomitable vitality of the fruit of Chinese wolfberry, the traditional Chinese medical science think that taking the fruit of Chinese wolfberry can help human body to strengthen energy, nourishing liver and kidney, benefiting shrewd head, the increase immunity that nourishes blood, and the effect that the fruit of Chinese wolfberry adapts to most is exactly an antifatigue.Antifatigue: matrimony vine can be improved hydroxyproline concentration, thereby improves muscular strength, and fatigue capability is strengthened.

5, protection liver: Fructus lycii P.E has certain effect to protecting liver and fatty liver.Reason is that matrimony vine contains a kind of active ingredient one sweet theophylline, and it is effective to the treatment liver.Pharmacological research shows that sweet theophylline has the fat of inhibition to deposit, and promotes the effect of liver cell regeneration in liver cell.Concerning the liver patient, the sweet theophylline in the fruit of Chinese wolfberry can prevent that fattiness stores in the liver, and lipotropic effect is arranged, and contained chlorophyll helps the liver detoxification effect in the matrimony vine, can also increase and improve liver function simultaneously.Chronic hepatitis patients, especially fatty liver patient.

6, reducing blood lipid: Fructus lycii P.E can reduce the rat cholesterol, and the cholesterol that can obviously suppress high cholesterol and the caused rabbit anteserum of lard feed increases.

7, hypoglycemic: wolfberry fruit extract can reduce rat blood sugar significantly enduringly, improves sugar tolerance.

8, control age-related macular degeneration: the fruit of Chinese wolfberry has the easypro liver effect that makes eye bright.To chronic hepatitis, central retinitis, optic atrophies etc. are evident in efficacy.Ageing disorders such as old organ senescence had very strong improvement effect.Discover that the fruit of Chinese wolfberry helps to reduce the generation of old degeneration maculopathy.

9, anti-apoptotic: Apoptosis is called apoptosis again.Interrelated data shows that brain normally has 10 nerve cell deaths every day approximately, and the human body new nerve cell of can not regenerating.When the fast a large amount of apoptosis of cell, early ageing, weak, film attenuation and atrophy can appear in the people, skin kraurosis, and with a haggard face, memory and thinking ability descend, even it is slow-witted old disease to occur.

Apoptosis except with occur outside the Pass pathology and inherent cause have, a large amount of apoptosis fast and two factors are closely-related; First free radical is made the oxidation reaction of use free radical with old and feeble closely related, causes that the free radical of cellular oxidation all can cause Apoptosis.Studies confirm that, " cerebroside ester, " that the fruit of Chinese wolfberry contains is that compound sugar combines formed glycosides with the ceramide terminal hydroxyl, owing to cerebroside ester is combined into by hydrophilic glycosyl and lipophilic ceramide, be one of important component of cell membrane, can keep cyto-architectural integrality.Simultaneously, cerebroside ester can form hydrogen bond network widely in biomembrane polarity and nonpolar interface.This is to constitute the constitutionally stable key factor of biomembrane stratiform.

According to another report, Ca2+ extensively raises in the thymocyte that LBP DEX capable of blocking induces, and suppresses the dna segmentization that DEX induces, and inhibitory action has dose dependent, its mechanism of action may pass through to reduce the wide concentration of cell C for LBP, suppresses the endonuclease enzyme activation, thus the blocking-up Apoptosis.

10, antitumor: the LBP-X combination with radiotherapeutic has certain radiosensitizing effect to acute weary oxygen tumour cell.LBP-X has in the non-specific antitumor and specificity antineoplastic process macrophage certain activation is all arranged.With S180 lotus knurl Bal6/C mouse is model, discover, LBP-X can recover and improve mice with tumor spleens cell number and activating T cell multiplication capacity, promotes the active and TNF secretion level of NK, and the prompting LBP-X can strengthen cellular immune function makes it suppress the dominant mechanism of tumor effect.

11, beauty treatment is preserved youthful looks: so the anti-ageing lipid that can suppress deposits in liver cell, promote liver cell new life's effect.Matrimony vine contains carotene, and compositions such as thiamine, riboflavin, Yan acid have moist skin, and the look of preserving youthful looks is the good merchantable brand of beauty treatment.The fruit of Chinese wolfberry can improve the ability to take oxygen of skin, can play the effect of skin whitening.

It is reported that Britain, French medical science man discover that the fruit of Chinese wolfberry contains a kind of vitamin of not meeting, and is named as " vitamin ", is also referred to as the vitamin of preserving youthful looks.So Fructus lycii P.E has extremely strong skin maintenance effect.

(4), hide fern fiber crops (Potentiua anserpina), have another name called long-lived fruit, panax ginseng fruit.Tibetan language " tall and erect Lao Sha monk ".The fern fiber crops are rose family Potentilla perennial herb wild plant.Be distributed widely in the various places, Qinghai, be grown in the grassy marshland high above sea level of 1700-4300 meters of Qinghai-Tibet Plateans, near the alluvial flat, in the extremely strong area growth of moistening cold, output height, quality better are the medicine-food two-purpose good merchantable brands.Fern fiber crops nature and flavor are sweet flat, have higher medical value, the intestines tonifying spleen of being good for the stomach, promote the production of body fluid to quench thirst, the effect of blood-enrich, be called " tall and erect Lao Sha monk " the Tibetan medicine, Chang Yiqi is used as medicine.Its modern medicine study think have the invigorating the spleen of enriching blood, promote the production of body fluid to quench thirst, dampness removing.Be used for the treatment of anaemia, malnutrition, effects such as insufficiency of the spleen diarrhoea.

The fern fiber crops are plateau rose family wild plants, and have abundant nutritive value, contain starch, protein, fat, inorganic salts and vitamin.Also contain elements such as tannin, crude fibre, multiple sugar, magnesium, zinc, calcium, iron in addition.Long food truly has effect of prolonging life, Gu this, by people's good reputation " panax ginseng fruit ".

(5), honeysuckle (JAPANESE HONEYSUCKLE) has another name called FLOSLONICERAE.Be the caprifoliaceae plant bud of honeysuckle.Cold in nature, the flavor sweet.Return lung, the heart, stomach warp.Has effect clearing heat and detoxicating, wind-heat dissipating.Be used for swollen sore, larynx numbness, anemopyretic cold, warm disease heating, the erysipelas etc. treated of pain.

Chemical analysis: contain rhinoceros grass element, inositol, chlorogenic acid, isochlorogenic acid, ginkgolic acid, cupreol, stigmasterol, cupreol-D-or the like glucosides, stigmasterol-D-or the like glucosides and volatile oil, various trace elements.

Pharmacological action: 1, antipathogen: the honeysuckle water decoction all has certain inhibitory action to typhoid bacillus, paratyphosum Bacterium, staphylococcus aureus, Escherichia coli, shigella dysenteriae, streptococcus pneumonia, diplococcus meningitidis, tubercle bacillus, popular virus etc.Body is interior, experiment in vitro shows, the honeysuckle decoction all has prevention and therapeutic action to influenza virus and herpesviral; The mouse infection influenza virus there is the death rate of reduction and suppresses pulmonary inflammatory effect.Intestines abdominal cavity or intravenous injection can obviously reduce poisoning and the death of mouse and rabbit due to the Pseudomonas aeruginosa endotoxin.

2, antipyretic and anti-inflammatory: the rat paw edema that Honegsukle flower P.E on Carrageenan, egg white etc. cause has inhibitory action, also has significantly imperviously to go out and synergistic effect.Honeysuckle has tangible refrigeration function in addition.

3, anti-endogenous toxic material: the honeysuckle lumbar injection can make accepts LD ₅₀The Pseudomonas aeruginosa endotoxin or the mouse survival rate of Pseudomonas aeruginosa reach more than half.Quiet notes honeysuckle distillate has therapeutic action to the rabbit of the green bacillus endotoxicosis of purulence, can improve that its leucocyte that causes reduces and the body temperature reduction.

4, improve immunologic function: the honeysuckle decoction can increase the phagocytic activity of leucocyte, inflammatory cell.Extract can significantly improve the phagocytic function of scalding mouse macrophage, improves the function that the mouse liver Kupffer is removed prepared Chinese ink, makes low macrophage antigen pass function and is recovered.

5, protect the liver: honeysuckle raises to the poisoning mice serum glutamic pyruvic transminase that carbon tetrachloride causes chmice acute, subacute hepatic injury and D-Gal to cause at decoction, and oil such as reduction liver obviously alleviate the hepatic pathology degree of injury by triol content.

6, reducing blood lipid: the honeysuckle decoction gavages, and can reduce the absorption of its intestines inner cholesterol, reduces content of cholesterol in the blood plasma.Experiment in vitro finds that honeysuckle can combine with cholesterol.

7, anticancer: honeysuckle water and steeping in wine extract are to sarcoma S ₁₈₀And EC has tangible CDCC.Flos Lonicerae extractive solution is oral to have slight prevention effect to the experimental gastric ulcer of rat experiment.

(6) hide Radix Glycyrrhizae (Gan Cao), be glycyrrhizic legume, glycyrrhiza inflate bat or glycyrrhiza glabra root and rhizome.The property flat flavor sweet.The thoughts of returning home, spleen, lung, stomach warp.Have invigorate the spleen and benefit qi, effect such as clearing heat and detoxicating, expelling phlegm and arresting coughing, relieving spasm to stop pain, all medicines of blending.Be used for weakness of the spleen and the stomach, lassitude hypodynamia, palpitation, coughing with a lot of sputum, Guang abdomen, carbuncle sore tumefacting virus, cushion toxicity.

Chemical composition: mainly contain glycyrrhizin, glycyrrhizic acid, different enoxolone, licorice root lactone, liquoric acid, glycyrrhizin, β one sitosterol, forulic acid, amino acid, salicylic acid, oleanolic acid, alkaloid, choline, betaine.

Pharmacological action:

1, anti-inflammatory: carry in the extract of licorice root various enoxolone samples all have antiinflammatory action.Acute and chronic inflammation such as the auricle lump that licorzinc causes mouse dimethylbenzene, the swelling of Irish moss colloidality toes, adjuvant-induced arthritis, granuloma induced by implantation of cotton pellets all have antiphlogistic effects.Its mechanism is that glycyrrhizin produces antiinflammatory action by the formation that the metabolism that suppresses neutrocyte reduces active oxygen.

2, antibiotic, desinsection: glycyrol extract and Sodium glycyrrhetinate all have inhibitory action external to staphylococcus aureus, tubercle bacillus, Escherichia coli, Amoeba and trichomonad, but under the situation that has blood plasma to exist, it suppresses bacterium and weakens to some extent with the effect of killing Amoeba.

3, antiviral: licorice polysaccharide is to vesicular stomatitis virus, and adenovirus III type, herpes simplex virus I-type and vaccinia virus all have obvious inhibitory action.Glycyrrhizic acid can destroy the AIDS virus cell of test tube, and glycyrrhizic acid plays a role by recovering t helper cell.New Polyphenols in the Radix Glycyrrhizae of Qinghai demonstrates the proliferation inhibiting effect to AIDS virus when low concentration.

4, antitumor: glycyrrhizin, liquiritin can make rat ascites liver cancer and mouse ehrlich ascites cell produce morphologic variation, and its mono-ammonium all has inhibitory action to mouse EC and sarcoma.Enoxolone and derivative 3-oxygen thereof-18-a enoxolone is inhibited to the Oberling-Guerin myeloma of rat implantation, and its derivative also has anti-mouse leukemia effect.Experiment in vitro proves that licochalcone A can suppress the P that 12 oxygen-myristoyl euphorbia diterpenoids alcohol acetate quickens ³²The permeability behavior of HeLa cell phosphatide, this and anti-tumor in vivo effect are parallel relation.

5, antiulcer :-extract of licorice root, glycyrrhizin etc., the zoopery ulcer that ligation pylorus, histamine are formed has the obvious suppression effect.Licorzinc irritate stomach to the rat chronic acetic acid type, stress type, reserpine type, pyloric ligation ulcers gastric ulcer have significant protective effect; can make the area and the volume-diminished of gastric ulcer; the hyperemia and the extent of hemorrhage of gastric mucosal damage degree, ulcer surface alleviate, and show the good dosage and the relation of effect.The licorzinc antiulcer action is with promoting that the fibrocyte synthetic fibers are relevant with matrix.

6, gastric acid inhibitory liquid extract of liquorice filling stomach can directly adsorb hydrochloric acid in gastric juice, can reduce normal dogs and experimental ulcer rat hydrochloric acid in gastric juice amount: the PM100 duodenal administration can suppress gastric secretion, and the gastric secretion that albumen, histamine and Carbamoylcholine are caused has remarkable inhibitory action.

7, analgesia: the liquorice beverage decoction is to having obvious inhibitory action at body uterus normal activity, and can suppress PGF _2aDue to rabbit in the strong contraction in body uterus, to by PGF _2a, the mouse writhing reaction that causes of the rat writhing response that brings out of oxytocins and acetic acid has obvious inhibitory action.

8, to the rat and the mouse of heating, rabbit has refrigeration function respectively for analgesic calm enoxolone and glycyrrhizin, and enoxolone 125mg/kg presents inhibitory action to central nervous system of mice, can cause calmness, hypnosis, body temperature reduction and respiration inhibition etc.

9, anti-oxidant: flavonoids of Glycyrrhiza can obviously suppress the MDA that the even oar of Mouse Liver causes and raise under the vibration incubation conditions, superoxide anion and hydroxy radical are had scavenging action, its to lipid peroxy when the antagonism of free radical be proved.Its main component it, liquorice chalcone has tangible lipoid peroxidization resistant, and hydroxy radical is had direct scavenging action.And the anti-promoting effect of licorice and antioxidation have certain relation.

10, reducing blood lipid, antiatherosclerosis: glycyrrhizin intramuscular injection, experimental rabbit hyperlipidemia there is tangible effect for reducing fat, low dose of glycyrrhizin (2mg/ day) can make the cholesterol of experimental atherosclerosis rabbit reduce within a certain period of time, atherosis degree alleviates, 20mg/ day can stop main artery and crown development of atherosclerosis, but at 40mg; Then invalid during purpose dosage.The rabbit of enoxolone salt pair hyperlipemia rat and experimental atherosclerosis has cholesterolemia, lipoprotein and the β-lipoprotein triglycerides effect of reduction, and its reducing blood lipid and study of anti-atherogenic effect are stronger than the polymerization saponin.

11, suppress platelet aggregation: isolated 3 one fragrant coumarin derivative Gu 1 from Radix Glycyrrhizae, can suppress platelet aggregation, suppress the blood platelet phosphodiesterase activity, and increase blood platelet CAMP concentration.Liquorice chalcone first and second can suppress 12 monohydroxy 1,8,10-heptadecenoic acid and TBs ₂Form; Inhibition is by the increase of the free calcium concentration of thrombin induction, and dose-dependence is arranged.

12, protect the liver: liquid extract of liquorice has obvious hepatoprotective effect to hepatic lesion.Glycyrrhizin can obviously stop the rising of carbon tetrachloride poisoning rat glutamic-pyruvic transaminase, can also reduce accumulating of the interior triglycerides of liver.Enoxolone can also the strong inhibition carbon tetrachloride free radical and the generation of lipid peroxide, suppress Ca ²⁺Flow into caused primary cellular defect in the cell.

12, cough-relieving apophlegmatic: the oral pharyngeal mucosa that can cover inflammation of extract of licorice root and mixture glycyrrhiza, relax the stimulation of inflammation, the performance antitussive action.Antitussive effect of Radix Glycyrrhizae and anti-inflammatory are irrelevant, but produce by maincenter.Radix Glycyrrhizae can also promote throat and bronchial secretion, makes the easy expectoration of phlegm, presents the eliminating phlegm and relieving cough effect.

13, anti-arrhythmia: 18 β-Sodium glycyrrhetinate can resist rabbit VA due to chloroform-adrenaline, rat VA time of occurrence due to the prolongation calcium chloride, slow down rat and rabbit heart rate, the heart rate that part is resisted isoprel adds fastoperation.Glycyrrhiza total flavonoid can prolong the mouse arrhythmia cordis incubation period that aconitine brings out, and reduces the mouse ventricular fibrillation positive rate that chloroform brings out.

14, antiallergic action: the glycoprotein components Lx in the Radix Glycyrrhizae can suppress the generation of mouse immuning ball protein E; Glycyrrhizin can suppress the release that target cell takes off particle and histamine; Licorice and glucoside have the effect of pair anti-acetylcholine and tissue, and can suppress antibody and the generation of immunity note Yi Xibao, stop the processing of macrophage to antigen.Licorice Lx can obviously reduce the incidence and the death rate that the mould thiazole causes the irritated body gram of cavy, and the mouse allelgic shock is also had protective effect.

15, immunoregulation effect: glycyrrhizin can make the weight of some immune organs significantly increase, and normal mouse is significantly improved vein carbon particle clearance index, and the nospecific immunity humidification is arranged.Glycyrrhizic acid can promote the generation of r-interferon and interleukins-2, strengthens natural killer cell activity.The crude extract Lx of Radix Glycyrrhizae then has immunosuppressive action, can suppress the immune response of macrophage, its act on macrophage with engulf relevant enzyme, reduce the antigenic information amount and show its immunosuppressive action.

16, to the influence of sex hormone: glycyrrhizin, enoxolone can suppress the accretion to teenage animal uterus such as estrogen, and adrenalectomize or ovary still have same purpose.Glycyrrhizin dosage increases can make estrogen action strengthen.Licorice has gonadotropic Effect, renders a service big than estradiol.Enoxolone has the effect that the mouse propagation gland produces stosterone that suppresses.

17, improve auditory function: enoxolone hemisuccinic acid ester disodium has the effect that improves the guinea pig inner ear auditory function.After glycyrrhizic acid and streptomysin alkalescence group are combined into streptomycin glycyrrhizinate, do not influence its antibacterial activity, but can alleviate the infringement of streptomysin vestibular nerve.

18, detoxifcation: glycyrrhizin, glycyrrhizic acid, extract of licorice root, ammonium glycyrrhetate etc. all have detoxication.Its effect precipitates, adsorbs by the mode of physics, chemistry, and approach such as reinforcement liver detoxification function realize.Radix Glycyrrhizae and preparation thereof are foretold poison, food poisoning, interior metabolism product poisoning and bacteriotoxin etc. to medicine L, and certain detoxication is all arranged.Living Radix Glycyrrhizae can make Mouse Liver spare the frame cytochrome P _-450Content obviously increases, and shows liver drug enzyme is had inducing action, may be one of its detoxifcation mechanism.

(7) green tea (Green tea) tealeaves.Tealeaves is in the history in existing thousands of years of China, and five continents all over the world except its special tempting delicate fragrance mouthfeel, are favorite drink in people's daily life because of its abundant health-care efficacy forms.The health-care effect of tea just enjoys blue or green Lay since ancient times.Record just has 20,219 effects in the medical book in 16 kinds of Gus of China.Just had before more than 4700 year " shennong went into the mountains collecting, tasting and testing different kinds of herbs to be used as medicine, day meets 72 poison, tea and separate it " record.The record of " all medicines are the medicine of each disease, and tea is the medicine of all kinds of diseases and ailments " is just arranged in the Tang Dynasty supplement to the Herbal that Chen Cangqi is write.Along with science and technology development, the health-care components in the tealeaves also more and more is familiar with by people, and the long-term drinking tea set has effect of prolonging life.

Tealeaves is the leaf of Theaceae plant tea tree, processes through science.Cool in nature, sweet-bitter flavor, go into the heart, liver,spleen,kidney, the lung Five Classics.Has the refresh oneself, effect such as relieving restlessness is drunk, reduces phlegm, helped digestion, diuresis, detoxifcation.Modern study thinks, tea set have reducing blood lipid, hypotensive, hypoglycemic, lose weight, delay senility, radiation proof, crease-resistant beauty functions.

Chemical composition: tealeaves contains compositions such as amino acid, Tea Polyphenols, chlorophyll, V, mineral matter, theophylline.

Physiological function: 1, the benefit of refreshing oneself is thought: modern medicine study thinks, tealeaves refresh oneself that benefit thinks mainly by 3 factors, the one, the caffeine in the tealeaves can excited higher nervous center, makes mental excitation, ideologically active, metabolism is accelerated; The 2nd, the molysite in the tea is in the immeasurable effect of blood circulation; The 3rd, the aromatic substance in the tea can produce refreshing effect to the mind.

2, hypotensive: the caffeine in the tealeaves, theophylline can direct excited hearts, the expansion coronary vasodilator, and peripheral vessel is had direct effect.Tea extract nutgall catechin, gallate 0.01mg/kg intravenous injection can make the anesthetized rabbit blood pressure obviously descend, and keep the long period; When containing the water-soluble part 5mg/kg of the going out intravenous injection of manganese in the water extract, only make rabbit produce a blood pressure drops of crossing property.

3, reducing blood lipid: prevent and treat artery sclerosis: the Tea Polyphenols in the tealeaves can suppress quail serum total cholesterol (TC), the rising of triglycerides (TG) and low density lipoprotein cholesterol (TC/HDL-C) has the obvious suppression effect, its mechanism of action may make by suppressing the absorption of intestinal tube tissue to cholesterol, suppress the synthetic of body inner cholesterol, reduce the activity of lipoprotein lipase, promote adrenergic lipase active of leading to, stop and promote in the food that cholesterol is converted into cholic acid unsaturated fat acid oxidase, thereby promote lipid to decompose, and excrete with ight soil.

4, hypoglycemic: as to it is reported that green tea can prevent diabetes.Studies confirm that Tea Pigment can suppress the big white mouse diabetes of being led to by streptozotocin (STZ), reduce blood sugar.Also may have protection-cell with Tea Pigment is subjected to the effect of STX virus relevant.

5, antitumor: the green tea water extract is to EC, and the meat cancer has the obvious suppression effect; The outer stomach cancer cell of cultivating of body there is tangible lethal effect; Its mechanism of action is that the green tea composition can reduce the tumour cell division number, and tangible lymphatic infiltration is arranged.Its cancer suppressing action is relevant with raising body antitumor immune.Prove that according to another great deal of experiment data green tea is to the blocking effect that is formed with of human carcinogen's property nitroso compound, and effectively suppressor and DNA covalent bond.

6, anti-oxidant, anti-ageing: the Tea Polyphenols in the green tea has the effect of removing oxygen radical, caffeine, catechin, epicatechin have the stronger antioxidation of catching, the oxygen radical that can obviously suppress hypoxanthine and xanthine oxidase reaction generation causes that the tannic acid in the tea can suppress the lipid oxidation of liver cell mitochondria, and it acts on strong VE.The life span of drosophila melanogaster experimental result shows that green tea has tangible anti-aging effects.According to another report, Japanology thinks that to the anti-ageing mechanism of tealeaves Tea Polyphenols can effectively block free radical activity, catechin has anti-oxidant, anti-aging effects.

7, anti-bacteria and anti-virus: tealeaves has certain inhibitory action to diplococcus meningitidis.Tealeaves medicinal extract or decoction all have inhibitory action to various shigella dysenteriaes, salmonella, staphylococcus aureus, white marquis bacillus, bacillus anthracis, typhoid bacillus in test tube.The detoxifcation of tealeaves is many-sided.Compositions such as Tea Polyphenols can with the protein combination of bacterium, make bacterioprotein solidify sex change, cause bacterium death, and detoxify with this.

8, diuresis defaecation: the diuresis function of tea mainly be alkaloid in the tea, particularly aminophylline in action.Theophylline nephrectasia fine vascular on the one hand promotes urine to form, and stimulates bladder, the discharge that helps urinating on the one hand.The defecating feces excretion of tea mainly is contraction and the creeping effect that Tea Polyphenols can strengthen large intestine.

9, permanent tooth preventing decayed tooth: tea tree be a kind of can be from soil the plant of enrichment fluorine element, the content of fluorine is up to 250-1600ppm in the tealeaves, fluorine has the permanent tooth anticaries action.Tea polyphenolic compounds can kill pathogen in the teeth space in addition, not only tooth is had protective effect, and removes halitosis.

10, protection brain cell: it is reported that Instite of Biophysics, Chinese Academy of Sciences's brain and people know new studies show that in research center, the polyphenol in the green tea can effectively be protected dopamine neuron, drinks green tea cerebral tissue is shielded.The researcher with regard to green polyphenol to being afraid of the influence of golden gloomy animal model; discover; green tea polyphenols can be protected dopamine neuron, and this mechanism of action realizes by suppressing ROS-NO approach, and ROS-NO approach is the cell death major reason that promotes to be afraid of golden gloomy disease patient.

11, anti-curing arthritis: it is reported that the polyphenol in the green tea can effectively be alleviated rheumatic arthritis, inducing mouse is suffered from after the arthritis in a research, treats again, and the result shows that green tea polyphenols can be that the ratio that arthritis ache takes place reduces 50%.

12, radiation proof: drinking tea helps vision protection and healthy.Drink tea and to prevent the blurring of image and recover eyesight.Many researchs are thought radiation-resistant effect of having drunk tea can reduce the radiation of computer fluorescent screen X ray to health.Be rich in the green tea compositions such as Tea Polyphenols and lipopolysaccharides can adsorb and catch radioactive substance and with excrete after it combines.

In addition, tealeaves also have anti-sudden change, antiallergy, effect such as sober up.

Comprehensively described, this product has effects such as antifatigue, anti-anoxic, enhancing immunity.

Manufacture craft:

1, raw material mixes pulverizing: the raw material in will filling a prescription earlier mixes to be pulverized.

2, raw material lixiviate: raw material lixiviate water weight ratio is that 1:6-8 soaks to boil in 2 hours and filtered to get filtrate one in lixiviate 1-3 hour, two, twice filtrates that filtered to get filtrate in 1-2 hour of the lixiviate raw material filter residue water boil lixiviate that adds 1:4-6 for the second time are mixed into the raw material extract.

3, allotment mixes: the water that takes a morsel dissolves auxiliary material with the raw material leaching liquor and mixes, and stirs.

4, filter: deployed raw material leaching liquor is sent into filter filter.

5, filling and capping: the raw material leaching liquor after will filtering is sent into bottle placer: can and beverage bottle, and carry out capping.

6, sterilization:, put into 80-100 ℃ boiling water sterilization 20-30 minute with the beverage bottle of filling and capping.

7, test package: with the semi-finished product beverage after the sterilization, the packing that is up to the standards becomes formal product.

Raw material mixing pulverizing is the process for whole beneficiating ingredients in the lixiviate raw material.Raw material lixiviate operation is the process that adds composition in a certain proportion of flooding raw material at the raw material of pulverizing.It is auxiliary material to be dissolved in low amounts of water mix with the raw material leaching liquor that allotment mixes, and the operation that stirs.Filtering operation is in order to filter the process of raw material leaching liquor impurity.The can operation is that the raw material leaching liquor after filtering is sent into the bottle placer can, and carries out the process of capping.Sterilization process is in order to kill microorganism in the beverage and other harmful components, with the process of the quality that guarantees product.The semi-finished product beverage of test package operation after with sterilization is up to the standards, and packing becomes the process of product.

Six, quality standard:

1, physical and chemical index:

Safranine red-spotted stonecrop g/100ml 6

Hide matrimony vine g/100ml 5

Hide sea-buckthorn g/100ml 4

Hide fern fiber crops g/100ml 5

Hide Radix Glycyrrhizae g/100ml 3

Honeysuckle g/100ml 4

Green tea g/100ml 1

2, instructions of taking: every day three times, each 100-200ml

Seven, embodiment: get 6 kilograms of safranine red-spotted stonecrops, hide 5 kilograms of matrimony vines, hide 4 kilograms of sea-buckthorns, hide 5 kilograms of fern fiber crops, hide raw materials such as 3 kilograms in Radix Glycyrrhizae, 4 kilograms of honeysuckles, 1 kilogram of green tea, raw material in the prescription is mixed pulverizing, the water logging bubble that adds 1:8 boiled lixiviate in 2 hours and filtered to get filtrate one in 2 hours, the raw material of lixiviate for the second time filter residue adds 1.5 hours secondaries that filter to get filtrate of water boil lixiviate of 1:6, twice lixiviate filtrate is mixed into the raw material leaching liquor, the raw material leaching liquor adds other auxiliary material and dissolves, bottle placer can and capping are sent in the filtration that stirs.Sterilization is 20 minutes in 85 ℃ boiling water, and the packing that is up to the standards becomes formal product.

Claims (2)

1, rhodiola rosea cold tea, manufacture craft is: raw material mix to be pulverized, the secondary flooding, and allotment mixes, and stirs, filtration, filling and capping, sterilization, test package become formal product.

2, process conditions are: 6 kilograms of safranine red-spotted stonecrops, hide 5 kilograms of matrimony vines, hide 4 kilograms of sea-buckthorns, hide 5 kilograms of fern fiber crops, hide 6 kilograms in Radix Glycyrrhizae, 4 kilograms of honeysuckles, green tea waits raw material for 1 kilogram, the water logging bubble that raw material in the prescription mix to pulverize is added 1:8 boiled lixiviate in 2 hours and filtered to get filtrate one in 2 hours, the water boil lixiviate of the raw material of lixiviate for the second time filter residue adding 1:6 1.5 hours, filter to such an extent that filter two, twice filtrate is mixed into the raw material leaching liquor, the raw material leaching liquor adds other stone and dissolves, filtration stirs, send into bottle placer can and capping, sterilization is 20 minutes in 85 ℃ boiling water, and the packing that is up to the standards becomes formal product.