CN103784600A - Chinese herbal medicine oral solution for enhancing immunity of racoon dog - Google Patents

Abstract

The invention relates to a Chinese herbal medicine oral solution for enhancing immunity of a racoon dog. The Chinese herbal medicine oral solution is prepared from the following Chinese medicinal herbs in parts by weight: 1-2 parts by weight of the fruit of Chinese wolfberry, 1-2 parts by weight of glossy privet fruit, 1-2 parts by weight of the fruit of Chinese magnoliavine, 1-2 parts by weight of oldenlandia diffusa, 1-2 parts by weight of plantain herb, 1-2 parts by weight of selfheal, 1-2 parts by weight of sweet wormwood and 1-2 parts by weight of echinacea. The Chinese herbal medicine oral solution for enhancing the immunity of the racoon dog is prepared through the steps of crushing the Chinese medicinal herbs, burdening, boiling with water and digesting, concentrating, and packaging. The Chinese herbal medicine oral solution for enhancing the immunity of the racoon dog has better effects of enhancing the disease resisting and preventing capacities of the racoon dog and enhancing the immunity.

Description

Strengthen the Chinese herbal medicament oral liquid of recoon dog immunity

Technical field

The present invention relates to a kind of Chinese herbal medicament oral liquid that strengthens recoon dog immunity.

Background technology

In recent years, in recoon dog aquaculture, existing malnutrition, stressors and hormone medicine use etc. declines immunity of organisms, cause having various virosiss as viral enteritis, canine distemper, bacterial disease is as the generation of colibacillosis, salmonellosis and parasitic disease etc. and popular, and high incidence and mortality rate cause certain economic loss to raiser.For reducing the generation of above-mentioned epidemic disease, effective measures are to activate body immune system, promote macrophage picked-up, process and transmit antigen, regulate and strengthen lymphocytic respond, increase lymphocytic quantity and promote it to reach maturity, improve immunity of organisms.At present, chemicals para-immunity reinforcing agent all exists toxic and side effects, drug residue and interim medication and does not reach the drawback of ideal effect to some extent.Chinese herbal medicine belongs to natural, nuisanceless medicine is international veterinary development trend.Therefore, the research of the Chinese herbal medicine of enhancing human body immunity power receives publicity increasingly.At present, on market, there is no the Chinese herbal medicament oral liquid sale that strengthens recoon dog immunity.

Summary of the invention

The object of the invention is to for the problems referred to above, a kind of Chinese herbal medicament oral liquid that strengthens recoon dog immunity is provided, this Chinese herbal medicament oral liquid can effectively improve recoon dog immunity and diseases prevention ability.

Technical scheme of the present invention is: a kind of Chinese herbal medicament oral liquid that strengthens recoon dog immunity, it is to be made up of the crude drug of following weight parts: Fructus Lycii 1-2 part, Fructus Ligustri Lucidi 1-2 part, Fructus Schisandrae Chinensis 1-2 part, Herba Hedyotidis Diffusae 1-2 part, Herba Plantaginis 1-2 part, Spica Prunellae 1-2 part, Herba Artemisiae Annuae 1-2 part, Echinacea 1-2 part;

The preparation method of the Chinese herbal medicament oral liquid of this enhancing recoon dog immunity is:

Weighting raw materials Fructus Lycii, Fructus Ligustri Lucidi, Fructus Schisandrae Chinensis, Herba Hedyotidis Diffusae, Herba Plantaginis, Spica Prunellae, Herba Artemisiae Annuae, Echinacea respectively;

Above-mentioned raw materials medicine is ground into respectively to Chinese medicine coarse powder;

Fructus Lycii, Fructus Ligustri Lucidi, Fructus Schisandrae Chinensis, Herba Hedyotidis Diffusae, Herba Plantaginis, Spica Prunellae, Herba Artemisiae Annuae seven taste Chinese medicine coarse powder are mixed, soak 1 hour, water was opened slow fire boiling after 30 minutes, put into Echinacea, and slow fire decocts 10 minutes again; Filter; Decoct with water again secondary, each 20 minutes at every turn;

Filter, merges three times filtrate, concentrated, add 0.05% sodium benzoate as antiseptic, add water, stir evenly, filtration, fill, sterilizing, makes the Chinese herbal medicament oral liquid of this enhancing recoon dog immunity.

The preferred raw material medicines in portions by weight of Chinese herbal medicament oral liquid of this enhancing recoon dog immunity is: 2 parts of Fructus Lycii, 2 parts of Fructus Ligustri Lucidi, 1 part of Fructus Schisandrae Chinensis, 1 part of Herba Hedyotidis Diffusae, 1 part of Herba Plantaginis, 1 part of Spica Prunellae, 1 part of Herba Artemisiae Annuae, 1 part of Echinacea.

In the Chinese herbal medicament oral liquid prescription of this enhancing recoon dog immunity, each component pharmacological effect is as follows:

Fructus Lycii effect: Fructus Lycii another name Gou Qizi, Fructus Lycii red real, Radix Betae is sub, dark purple green pepper, Chinese holly hoof etc.Sweet in the mouth, property are put down, and return liver, kidney, lung meridian.Containing lycium barbarum polysaccharide, monosaccharide, carotene, betanin, fatty acid, proteins and peptides, vitamin B1, vitamin B2, vitamin B6, vitamin C, 18 seed amino acids, trace element.Tool nourishing the liver and kidney, beneficial intensive culture blood, improving eyesight removing nebula, effect of nourishing the lung to arrest cough.Cure mainly senilism senilism, blood deficiency and yellow complexion, interior-heat is quenched one's thirst, consumptive fever hectic fever due to YIN-deficiency, chronic consumptive disease cough, the diseases such as the few expectorant of dry cough.

Fructus Ligustri Lucidi effect: Fructus Ligustri Lucidi another name Fructus Ligustri Lucidi reality, Fructus Ilicis Purpureae, white beeswax tree, Fructus Ligustri Lucidi, Ilicis Purpureae etc.Sweet in the mouth, hardship, property is flat, returns liver, kidney channel.Containing oleanolic acid, acetyloleanolic acid, ursolic acid, mannitol, glycoside, Palmic acid, stearic acid, oleic acid, linoleic acid etc., and contain 11 kinds of elements such as potassium, calcium, magnesium, sodium, zinc, ferrum, manganese, copper, nickel, chromium, silver.There is enhancing immunologic function, rising peripheral cells, strengthen reticuloendothelial system phagocytic activity, strengthen the effect of cellular immunization and humoral immunization, have heart tonifying, diuresis, protect the liver, antibacterial action.Effect of tool liver and kidney tonifying, clearind deficient heat.For yin asthenia generating intrinsic heat, degradation under Abwehrkraft des Koepers.

Fructus Schisandrae Chinensis effect: Fructus Schisandrae Chinensis is called five Fructus Armeniacae Mumes, Fructus Zanthoxyli Plansipini etc.Sour in the mouth, warm in nature, returns lung, the heart, kidney channel.Containing volatile ingredients such as α-chamigrene, β-chamigrene, retinispora alcohol, β-bisabolences, and lignanoids and organic acid, also containing citral, chlorophyll, sterol, vitamin C, vitamin E, saccharide, resin and tannin etc.Bearberry Extract is astringent or styptic treatment for spontaneous sweating, effect of supplementing QI for promoting the production of body fluid, mind tranquilizing and the heart calming.Thirsty etc. for incessant chronic diarrhea, Tianjin wound.

Herba Hedyotidis Diffusae effect: Herba Hedyotidis Diffusae another name Herba Hedyotidis Diffusae, two leaf humulus, Herba Commelinae Bengalensis, Chinese Lobelia etc.Bitter but sweet flavor, cold in nature, GUIXIN, liver, spleen channel.Containing hentriacontane, stigmasterol, maloic acid, oleanolic acid, flavonoid glycoside and Bulbus Lilii Herba Hedyotidis Diffusae element etc.Staphylococcus aureus and dysentery bacterium are had to faint inhibitory action, and high concentration water's decoct can suppress the growth of bacillus pyocyaneus, Bacillus typhi, Bacillus proteus.Its crude extract aqueous solution energy Enhancement test animal Mus, rabbit and people's leukocytic phagocytosis.Effect of tool heat-clearing and toxic substances removing, dampness removing.For dyspnea and cough due to lung-heat, laryngopharynx swelling and pain, furuncle and phyma skin infection, dysentery enteritis, jaundice due to damp-heat etc.

Herba Plantaginis effect: another name wheel dish.Sweet in the mouth, cold in nature, return liver, kidney, lung, small intestine meridian.Do not have the fatty acids such as acid etc. containing plantagin, aucubin, ursolic acid, B-sitosterol, Palmic acid B-paddy steroid acid esters, brown tamarind sterol ester, vitamin B, vitamin C and plantaglucide, protein, succinic acid, adenine, choline, catalpol, stearic acid, arachidic acid, linolenic acid, Asia.Effect of tool clearing away heat and promoting diuresis, removing heat from blood, removing toxic substances.For heat-damp in summer dysentery, epistaxis, hematuria, laryngopharynx swelling and pain, carbuncle sore tumefacting virus etc.

Spica Prunellae effect: another name ferrum color grass, large headdress flower, June are dry, caput post etc.Cold in nature, sweet in the mouth, pungent, micro-hardship, enter liver, gallbladder meridian.Containing triterpenoid saponin, oleanolic acid, ursolic acid, globulariacitrin, hyperin, cis-caffeic acid, trans-caffeic acid, vitamin B ₁, vitamin C, vitamin K, carotene, resin, amaroid, tannin, volatile oil, alkaloid, water soluble salt etc.Decoct has inhibitory action to dysentery bacterium, Bacillus typhi, vibrio cholera, escherichia coli, Bacillus proteus, bacillus pyocyaneus and staphylococcus, streptococcus.Energy blood vessel dilating, its contained rutin has antiinflammatory action, and can reduce vascular permeability, reduces the effect of fragility.There is the effect of letting out clearly liver-fire, mass dissipating and swelling eliminating, heat-clearing and toxic substances removing, expelling phlegm for arresting cough, cooling blood for hemostasis, for bacillary dysentery etc.

Herba Artemisiae Annuae effect: another name Artemisia, careless Artemisia, smelly Artemisia, Conyza bonariensis (L.) Crong. etc.Bitter in the mouth, pungent, cold in nature.Return liver, gallbladder meridian, three warmers, kidney channel.Containing sesquiterpenoids, as arteannuin, arteannuin G, Qinghaosu I, Qinghaosu II, also containing volatile ingredient, as camphene, β-camphene, different artemisia ketone, levo-camphor, β-Flos Caryophylli alkene, nopinene and australene etc. still containing flavones ingredient, as Rhizoma Kaempferiae flavin, Quercetin, cyanidenon, also containing coumarin, Scopolean etc. and beta galactoside enzyme etc.Herba Artemisiae Annuae decocting liquid has stronger bacteriostasis to staphylococcus epidermidis, micrococcus catarrhalis, anthrax bacillus, diphtheria corynebacterium, and staphylococcus aureus, bacillus pyocyaneus, dysentery bacterium, tuberculosis stalk bacterium etc. are also had to certain inhibitory action.Arteannuin has direct killing action to plasmodium, and cellular immunization is had to facilitation.There is effect of clearing deficient heat, clearing away summer-heat preventing the attack (or recurrence) of malaria, dehumidifying parasite killing, for deficiency of YIN hectic fever hectic fever due to YIN-deficiency, outer temperature-sensing thermal heat-damp in summer, malaria, jaundice, dysentery etc.

Echinacea effect: Echinacea contains polysaccharide, flavone, caffeinic derivant, quintessence oil, polyacetylene, alkylamine and alkaloid.Echinacea has immunostimulation, reduces inflammation, and helps body to antibacterium and virus.Related tests shows to use Echinacea feeding animals, can improve the quantity of phagocytic activity and the periphery lymphocyte of neutrophil cell, the immunocompetence of effective stimulus animal body.

The Chinese herbal medicament oral liquid prescription foundation of this enhancing recoon dog immunity: this prescription is with strengthening vital QI to eliminate pathogenic factors, and enhancing body is disease-resistant, diseases prevention ability, and raising immunity is principle.In side, Fructus Lycii, Fructus Ligustri Lucidi, Fructus Schisandrae Chinensis can help body to antibacterium and virus, nourishing the liver and kidney, beneficial intensive culture blood, supplementing QI for promoting the production of body fluid; Herba Hedyotidis Diffusae, Herba Plantaginis, Spica Prunellae heat-clearing and toxic substances removing, cooling blood for hemostasis, anti-inflammation, Herba Artemisiae Annuae, the antibacterial parasite killing of Echinacea, regulate immunologic function.In side all medicine phases and, play altogether strengthening vital QI to eliminate pathogenic factors, invigorating the spleen and replenishing QI, promote the production of body fluid and nourish blood, improve effect of immunity of organisms and resistance against diseases.

That the Chinese herbal medicament oral liquid of this enhancing recoon dog immunity is applicable to is improper by pathogenic microorganism, drug use, malnutrition and the immunity of organisms that stress wait reason to cause low.The Chinese herbal medicament oral liquid of this enhancing recoon dog immunity can strengthen recoon dog immunity of organisms and resistance against diseases, has good effect.

The specific embodiment

Below in conjunction with embodiment, the invention will be further described.

Embodiment:

1, difference weighting raw materials (prepared slices of Chinese crude drugs): Fructus Lycii 200g, Fructus Ligustri Lucidi 200g, Fructus Schisandrae Chinensis 100g, Herba Hedyotidis Diffusae 100g, Herba Plantaginis 100g, Spica Prunellae 100g, Herba Artemisiae Annuae 100g, Echinacea 100g;

2, above-mentioned raw materials medicine is ground into respectively to Chinese medicine coarse powder;

3, Fructus Lycii, Fructus Ligustri Lucidi, Fructus Schisandrae Chinensis, Herba Hedyotidis Diffusae, Herba Plantaginis, Spica Prunellae, Herba Artemisiae Annuae seven taste Chinese medicine coarse powder are mixed, 7000 mL that add water soak 1 hour, and water was opened slow fire boiling after 30 minutes, put into Echinacea, and slow fire decocts 10 minutes again; Filter; 5000 mL that at every turn add water again decoct secondary, each 20 minutes;

4, filter, merges three times filtrate, be concentrated into about 900mL, add 0.05% sodium benzoate as antiseptic, add water to 1000mL, stir evenly, filtration, fill, sterilizing, makes the Chinese herbal medicament oral liquid of this enhancing recoon dog immunity.

Some test situation of utilizing the Chinese herbal medicament oral liquid of this enhancing recoon dog immunity to carry out are as follows:

1, the impact on recoon dog growth performance

More than choosing 50 ages in days, the fur of body weight in about 1.0kg is thick disorderly, 24 of poky recoon dogs, is divided at random 3 groups, 8 every group, weighs respectively the body weight of recoon dog before test.I group (blank group) is fed recoon dog basal diet; II group (astragalus polysaccharides matched group) basal diet adds 0.2% astragalus polysaccharides; III group basal diet adds 2% Chinese herbal medicament oral liquid.Feed 3 every day, and limit food is fed 0.1-0.2kg at every of every day, and duration of test records each group of feed intake, morbidity number and death toll, and records the body weight of dead recoon dog.Feeding after 30d, morning, 7-8 point was weighed on an empty stomach, recorded the body weight of each recoon dog.Test each group of medicine that does not all re-use other.

Use the Chinese herbal medicament oral liquid of this enhancing recoon dog immunity respectively to organize recoon dog clinical manifestation:

III group is at the 5d of test, and II group is at the 8d of test, and the recoon dog spirit of two groups becomes active, active, and appetite strengthens; After 10d, test recoon dog hair color light; I group does not have significant difference before and after test.At the 10-14d of test, I group has 2 morbidities, and without dead, II group and III group are without morbidity and dead.

The impact of the Chinese herbal medicament oral liquid of this enhancing recoon dog immunity on recoon dog growth performance, in table 1.

The each group of table 1 recoon dog growth performance unit: a kg g/d

Test grouping	Sample size	Starting weight	End is heavy	All feed intakes	Daily gain
						I group (blank group)	8	0.94±0.20 a	?1.50±0.04 aA	0.15	118.6±1.8 aA
II group (astragalus polysaccharides matched group)	8	0.97±0.23 a	?1.71±0.50 b	0.16	24±3.9 bB
						III group (this Chinese herbal medicament oral liquid)	8	0.93±0.13 a	?1.82±0.78 bB	0.18	0.29.6±5.5 cC

Note: same column lower case identical table differential is different not remarkable, the different significant differences that represent of same column lower case, capitalization is different represents that difference is extremely remarkable.

As shown in Table 1, duration of test, every daily gain 30g left and right of this Chinese herbal medicament oral liquid group.Whole duration of test, this Chinese herbal medicament oral liquid group daily gain compared with blank group and astragalus polysaccharides matched group increases, and effect is better.

This Chinese herbal medicament oral liquid can obviously increase recoon dog daily gain, contributes to recover the normal mental status, appetite and the speed of growth.This Chinese herbal medicament oral liquid can obviously improve the daily gain of recoon dog, has higher economic benefit.This Chinese herbal medicament oral liquid is better than astragalus polysaccharides to the therapeutic effect of recoon dog.

2, the impact on recoon dog viral enteritis antibody horizontal

Select 2 the monthly age 80 of recoon dogs, be divided at random the high, normal, basic test group of this Chinese herbal medicament oral liquid and matched group, 20 every group, grouping and immune programme for children are in table 2, hemagglutination inhibition test detects immune antibody and the results are shown in Table 3.

Table 2 test grouping and immune programme for children (unit: only)

Group	Process
		This Chinese herbal medicament oral liquid high dose group	Before and after immunity, 5d drinks this Chinese herbal medicament oral liquid by 2%, drinks continuously 10d
Dosage group in this Chinese herbal medicament oral liquid	Before and after immunity, 5d drinks this Chinese herbal medicament oral liquid by 1%, drinks continuously 10d
		This Chinese herbal medicament oral liquid low dose group	Before and after immunity, 5d drinks this Chinese herbal medicament oral liquid by 0.5%, drinks continuously 10d
Matched group	Matched group only does vaccine immunity, does not drink this Chinese herbal medicament oral liquid

Table 3 hemagglutination inhibition test detects immune antibody result (unit: only)

Time

High dose group antibody titer

High dose group meansigma methods

Middle dosage group antibody titer

Middle dosage cell mean

Low dose group antibody titer

Low dose group meansigma methods

Blank group antibody titer

Blank cell mean

14

1:8-1:64

1:27

1:8-1:32

1:26

1:8-1:32

1:25

1:8-1:16

1:22

21

1:16-1:128

1:48

1:16-1:64

1:46

1:16-1:64

1:44

1:16-1:32

1:42

28

1:32-1:128

1:49

1:32-1:128

1:48

1:32-1:64

1:46

1:32-1:64

1:44

60

1:32-1:128

1:49

1:32-1:128

1:48

1:16-1:128

1:46

1:16-1:64

1:44

90

1:32-1:64

1:46

1:16-1:64

1:46

1:16-1:64

1:46

1:8-1:64

1:44

After recoon dog immunological disease viral enteritis vaccine 14d, gather immune recoon dog determination of serum HI antibody titer.As shown in Table 3, when recoon dog immunological disease viral enteritis vaccine 21d, HI antibody reaches higher level, and when 28d-60d, antibody peaks, extremely slow decreasing after the rear 90d of immunity.Experiment shows, drinks the antibody horizontal that this Chinese herbal medicament oral liquid group produces compared with matched group higher and more even, wherein with high dose group for well.After high dose group immunity, recoon dog HI antibody titer can on average reach 1:49 when 28d-60d, indivedual high reached at 1:128 of recoon dog antibody horizontal.And after the immunity of blank group when 28d-60d recoon dog HI antibody titer can on average reach 1:44, what the highest recoon dog antibody horizontal was high just reaches 1:64.In the time of immune 90d, use 100ID ₅₀sMPV-11 Strain counteracting toxic substances, observes 15d, drinks high, normal, basic Chinese herbal medicament oral liquid group, no abnormality seen, and when 7d, high, normal, basic each group is respectively cutd open also no abnormality seen of 1 each internal organs of inspection, protective rate 100%; Matched group is shown in that indivedual recoon dog spirit is depressed, depressed by contrary unrest of hair, poor appetite, spirit, 10% recoon dog performance diarrhoea loose stool, and other no abnormality seen, 7d cuts open to examine and sees that 10% recoon dog small intestinal has hyporrhea, inside has exudate, protective rate 90% left and right.

Show that by every test the Chinese herbal medicament oral liquid of this enhancing recoon dog immunity all improves significantly to indices such as recoon dog growth promoter, body weight gains and viral enteritis antibody titers.

Claims (2)

1. strengthen a Chinese herbal medicament oral liquid for recoon dog immunity, it is characterized in that it is to be made up of the crude drug of following weight parts: Fructus Lycii 1-2 part, Fructus Ligustri Lucidi 1-2 part, Fructus Schisandrae Chinensis 1-2 part, Herba Hedyotidis Diffusae 1-2 part, Herba Plantaginis 1-2 part, Spica Prunellae 1-2 part, Herba Artemisiae Annuae 1-2 part, Echinacea 1-2 part;

The preparation method of the Chinese herbal medicament oral liquid of this enhancing recoon dog immunity is:

Weighting raw materials Fructus Lycii, Fructus Ligustri Lucidi, Fructus Schisandrae Chinensis, Herba Hedyotidis Diffusae, Herba Plantaginis, Spica Prunellae, Herba Artemisiae Annuae, Echinacea respectively;

Above-mentioned raw materials medicine is ground into respectively to Chinese medicine coarse powder;

Fructus Lycii, Fructus Ligustri Lucidi, Fructus Schisandrae Chinensis, Herba Hedyotidis Diffusae, Herba Plantaginis, Spica Prunellae, Herba Artemisiae Annuae seven taste Chinese medicine coarse powder are mixed, soak 1 hour, water was opened slow fire boiling after 30 minutes, put into Echinacea, and slow fire decocts 10 minutes again; Filter; Decoct with water again secondary, each 20 minutes at every turn;

Filter, merges three times filtrate, concentrated, add 0.05% sodium benzoate as antiseptic, add water, stir evenly, filtration, fill, sterilizing, makes the Chinese herbal medicament oral liquid of this enhancing recoon dog immunity.

2. the Chinese herbal medicament oral liquid of enhancing recoon dog immunity according to claim 1, is characterized in that it is to be made up of the crude drug of following weight parts: 2 parts of Fructus Lycii, 2 parts of Fructus Ligustri Lucidi, 1 part of Fructus Schisandrae Chinensis, 1 part of Herba Hedyotidis Diffusae, 1 part of Herba Plantaginis, 1 part of Spica Prunellae, 1 part of Herba Artemisiae Annuae, 1 part of Echinacea.