CN110721256B

CN110721256B - Traditional Chinese medicine composition for preventing and treating teniasis of laying hens and preparation method thereof

Info

Publication number: CN110721256B
Application number: CN201911003358.9A
Authority: CN
Inventors: 张振宇; 项朝荣; 张琳静; 惠永华; 苗培; 王梦艳; 王路; 李明; 张献芳; 逯晋忠; 穆伟峰; 关鹏; 王利梅; 王亦欣; 柳毅强
Original assignee: Jiyuan City Animal Product Quality Monitoring And Testing Center
Current assignee: Jiyuan City Animal Product Quality Monitoring And Testing Center
Priority date: 2019-10-22
Filing date: 2019-10-22
Publication date: 2021-11-16
Anticipated expiration: 2039-10-22
Also published as: CN110721256A

Abstract

The invention belongs to the technical field of veterinary medicines, and particularly relates to a traditional Chinese medicine composition for preventing and treating teniasis of laying hens and a preparation method thereof. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 50-70 parts of omphalia lapidescens, 30-50 parts of agrimony bud, 20-30 parts of sophora alopecuroides, 20-40 parts of rhizoma dryopteris crassirhizomae, 30-50 parts of rheum officinale, 30-50 parts of rhizoma cyperi, 30-40 parts of cubeb, 20-40 parts of betel nut, 20-40 parts of pumpkin seed and 30-50 parts of cherokee rose flower. The Chinese medicinal composition is used for carrying out treatment tests and prevention tests on the taeniasis of the laying hens, and the results show that the Chinese medicinal composition can effectively prevent the incidence and mortality of the taeniasis of the healthy laying hens and effectively reduce the breeding loss; can effectively reduce the mortality rate of the taeniasis-infected laying hens and improve the cure rate of the infected laying hens.

Description

Traditional Chinese medicine composition for preventing and treating teniasis of laying hens and preparation method thereof

Technical Field

The invention belongs to the technical field of veterinary medicines, and particularly relates to a traditional Chinese medicine composition for preventing and treating teniasis of laying hens and a preparation method thereof.

Background

The taeniasis of the laying hens is caused by taeniasis parasitized in the small intestine and has a plurality of varieties, China is mainly various taenias in theilex and lylis in thevinaceae, and the lyenias are most common and most harmful in clinic. The cestode development needs to take part in intermediate hosts such as scarab, housefly, ant and the like, when the intermediate hosts swallow the cestode eggs, the cestode eggs develop into cysticercus in the intermediate hosts, and the chickens are infected after swallowing the intermediate hosts.

Chickens of all ages can be infected with taeniasis, but chicks at the age of 14-17 days are the most susceptible. The mortality rate of chicks of 25-40 days old is highest. The chicks grow and develop slowly after being infected by tapeworm, acute enteritis and peritonitis are easy to occur, excrement mixed with blood is discharged, the production performance is greatly reduced, a large amount of death can be caused in serious cases, the survival rate is reduced, the egg yield of the laying hens after being sick is reduced, and great economic loss is caused to chicken breeders.

The main symptoms of the disease are: thin excrement or faint yellow excrement mixed with blood sample mucus is discharged, so that the spirit is poor, the feathers are fluffy and disordered, the appetite is poor, and the drinking water is increased; pale mucous membranes at the early stage, anemia, pale color of cocks and legs; the chicks grow and develop slowly after suffering from diseases, the egg yield of the laying hens is reduced after suffering from diseases, but sand shell eggs, faded eggs, thin eggshells and malformed eggs are not produced; when sick chickens are very thin, death often occurs. Stool color is often characterized as red or rust "sorghum-like stool" with white "sesamoid" tapeworm segments found on stool examination.

In the aspect of drug control, chemical drugs of thiobis-dichlorophenol, niclosamide, albendazole and praziquantel are mainly adopted for control at present, but the drugs are easy to have hepatotoxicity and enterotoxicity, and are prohibited to be used for chickens suffering from mixed infection with enteritis and enterotoxicity, and have drug residue risk after long-term use. The traditional Chinese medicine has the advantages of killing pests, stopping dysentery, improving immunity and other multi-target regulation, is green and safe, has small side effect and no toxic or side effect, and plays an increasingly important role in preventing and treating poultry parasitosis in recent years. In current actual clinical practice, only the toosendan fruit and tangerine peel powder can be used in the existing veterinary drug standards, but the effect is slow, and the clinical effect is not ideal. Some researchers also use the combination of betel nut, carpesium rhynchophyllum, rangooncreeper fruit, cyrtomium rhizome, brucea javanica, hairyvein agrimonia herb and bud, carpesium rhinacanthus, tobacco leaf and other medicaments to prevent and treat taeniasis, and tests show that the simple insecticidal prescription has a certain effect on improving the taeniasis excrement, but the cure rate and the protection rate are low, the effect is not obvious, and the application is not beneficial to product conversion. Therefore, research and screening of a Chinese veterinary medicament which is really efficient, safe, economical and convenient and can treat both principal and secondary aspects of diseases becomes an important measure for solving the current pain point of demand, so that the Chinese veterinary medicament has a higher market development value.

Disclosure of Invention

The invention aims to provide a traditional Chinese medicine composition for preventing and treating taeniasis of laying hens, and provides a corresponding preparation method.

Based on the purpose, the invention adopts the following technical scheme: a traditional Chinese medicine composition for preventing and treating teniasis of laying hens is prepared from the following raw material medicines in parts by weight: 50-70 parts of omphalia lapidescens, 30-50 parts of agrimony bud, 20-30 parts of sophora alopecuroides, 20-40 parts of rhizoma dryopteris crassirhizomae, 30-50 parts of rheum officinale, 30-50 parts of rhizoma cyperi, 30-40 parts of cubeb, 20-40 parts of betel nut, 20-40 parts of pumpkin seed and 30-50 parts of cherokee rose flower.

Preferably, the traditional Chinese medicine composition for preventing and treating teniasis of laying hens is prepared from the following raw material medicines in parts by weight: 60 parts of omphalia lapidescens, 40 parts of agrimony bud, 25 parts of sophora alopecuroides, 30 parts of rhizoma dryopteris crassirhizomae, 40 parts of rheum officinale, 40 parts of rhizoma cyperi, 35 parts of cubeb, 30 parts of betel nut, 30 parts of pumpkin seed and 40 parts of cherokee rose flower.

The preparation method of the traditional Chinese medicine composition for preventing and treating teniasis of the laying hens comprises the following steps: mixing Omphalia, herba Agrimoniae bud, herba Sophorae Alopecuroidis, rhizoma Dryopteris Crassirhizomatis, radix Rumicis, rhizoma Cyperi, fructus Litseae, Arecae semen, semen Cucurbitae, and flos Rosae Laevigatae at a certain proportion, pulverizing, sieving to obtain Chinese medicinal composition, and packaging.

Furthermore, the particle size of the obtained traditional Chinese medicine composition is not more than 60 meshes, and the traditional Chinese medicine composition is convenient to mix with materials.

When the traditional Chinese medicine composition is used for preventing teniasis of healthy laying hens, the healthy laying hens are continuously fed for 5 days according to the proportion of 300Kg of mixed materials of 1Kg of the traditional Chinese medicine composition; when the traditional Chinese medicine composition is used for treating cestodiasis-onset laying hens, the healthy laying hens are continuously fed for 5 days according to the proportion of 200Kg of mixed feed per 1Kg of traditional Chinese medicine composition.

The traditional Chinese medicine composition disclosed by the invention is prepared by purchasing commercially available products from all components, and the formula principle of the traditional Chinese medicine composition is as follows:

omphalia: is sclerotium of Polyporaceae plant Phyllolitae Cook.Et Mass. Bitter taste, cold nature and slight toxicity. Enter the stomach and large intestine. The functions are as follows: resolving food stagnation, killing parasites, and treating abdominal pain due to parasitic infestation and malnutritional stagnation. The main component is a protease called omphalin, the content of which is about 3 percent, and the omphalin is an effective component for expelling tapeworm, and the powder is suitable for being used after heating failure.

And (3) gemma agrimoninae: it is mainly Agrimonia Pilosa Ledeb.var.Japonica (Mia.) nakai. underground winter bud of Agrimonia of Rosaceae. Bitter and astringent taste, and cool in nature. It belongs to liver, small intestine and large intestine meridians, and is mainly used for treating taeniasis; however, the effective components are hardly dissolved in water, so it is not suitable to be decocted, and it is suitable to be powdered.

Sophora alopecuroide: is dried mature seed of Sophora alopecuroides L. Bitter taste and cold nature. The functions are as follows: clear heat and dry dampness, alleviate pain, kill parasites. Can be used for treating dysentery, leukorrhagia, eczema, intractable tinea, toothache, stomachache, and pyocutaneous disease.

Rhizoma Dryopteris Crassirhizomatis: mainly comprises rhizomes of Dryopteris crassirhizophila nakai of Dryopteridaceae, Osmunda japonica Thunb of Osmunda of Osmundaceae, Blechnia auriculata Blechni L of Blechnida of Meadowraceae, and rhizome of Blechnium orientale L of Blechni of Meadowraceae. Also named as phoenix-tail fern (the book draft Tu Jing). Bitter taste and cool nature. Has little toxicity. Enter liver and stomach meridians. The functions are as follows: to kill ascaris, tapeworm and pinworm, clear heat, remove toxicity, cool blood and stop bleeding. The main treatment is as follows: wind-heat type common cold, warm heat type macula, hematemesis, epistaxis, intestinal wind, hematochezia, and bloody dysentery.

Rhubarb root: is root of Rumex obtusifolius L. of Polygonaceae. Pungent and bitter in flavor; is cool in nature. It enters lung, spleen and large intestine meridians. The functions are as follows: clearing away heat and toxic material, cooling blood and arresting bleeding, removing blood stasis and detumescence, relaxing bowels and killing parasite. Unblocking intestine and purging heat, it is indicated for abdominal pain due to stasis.

Rhizoma cyperi: is the rhizome of Cyperus rotundus L. Pungent, slightly bitter and sweet in flavor and mild in nature. Enter liver and triple energizer meridians. The functions are as follows: regulating qi-flowing, resolving stagnation, relieving pain and regulating menstruation. The main treatment is as follows: disharmony between the liver and stomach, depression of qi, distending pain in the chest, abdomen and hypochondrium. Has the functions of warming the middle-jiao and promoting the circulation of qi, and walking without guarding.

And (3) cubeb: the product is dried mature fruit of Litsea cubeba (Lour.) Pers. Pungent flavor and warm nature. It enters spleen, stomach, kidney and bladder meridians. The functions are as follows: warming spleen and stomach for dispelling cold, promoting qi circulation and relieving pain; it is mainly indicated for vomiting due to stomach cold, abdominal cold pain, hernia of cold type and abdominal pain, etc., and has the actions of warming spleen and stomach, dispelling cold and alleviating pain, and promoting qi circulation.

Betel nut: is seed of Areca catechu L of Palmae. Bitter and pungent in flavor and warm in nature. Enter spleen, stomach and large intestine meridians. The functions are as follows: killing parasites, breaking accumulation, descending qi and moving water. The main treatment is as follows: parasitic malnutrition, food stagnation, abdominal distension and pain, diarrhea and dysentery with tenesmus.

Pumpkin seeds: is seed of Cucurbitaceae plant Cucurbita moschata Duch. Sweet in flavor and neutral in nature. Enter stomach and large intestine meridians. The functions are as follows: killing pests. The main treatment is as follows: tapeworm, ascaris, postpartum edema of hands and feet, pertussis, and the like. Cherokee rose flower: is flower of Rosaceae plant Rosa laevigata Michx. Sour and astringent taste; mild nature and no toxicity. It enters lung, kidney and large intestine meridians. Has the functions of astringing intestine, securing essence and killing parasites. Chronic diarrhea, dysentery, tapeworm, ascaris, etc. The "Rihuazi materia Medica" records: it can stop cold and heat dysentery, kill Bai and ascarid, etc.

After the laying hens are infected with tapeworm disease, common symptoms comprise inappetence, depression, anemia, emaciation, pale cockscomb and mucosa, red or rust-like excrement and the like, and most of the common symptoms comprise emaciation of laying hens, hepatosplenomegaly, punctate bleeding of intestinal mucosa and a large amount of white living polypides parasitizing from duodenum to jejunum.

The invention judges that the disease is caused by gastrointestinal damp-heat, diarrhea, emaciation, anorexia, lassitude and hypodynamia due to insufficient vital energy of spleen and stomach and complicated with parasitic damp-heat (tapeworm) accumulation. Based on the theory of veterinarian, it is judged that the disease belongs to the scope of the syndrome of parasitic infestation, parasitic tympanites, and insect tympanites, and the heat syndrome in the blood due to exogenous pathogenic insects. Pathogenic heat is in the blood system and predominates in consuming blood and moving blood. Consumption of blood causes consumption of essence, blood and body fluids, and bleeding is mainly manifested as bleeding due to blood heat breaking blood, red or rust-like feces, depletion of body fluids, and blood consumption with anemia, white crown, emaciation, etc.

The main treatment is detoxification and disinsection, and the auxiliary treatment is accumulation breaking and qi circulation promoting, so the invention selects omphalia as the monarch drug, named omphalia, wherein, the omphalia is quick as the omphalia and can be changed as the omphalia without remaining, the omphalia can attack and remove the accumulation, the omphalia can reach the spirit, and the emphasis is on disinsection and disinsection accumulation; the agrimony, the sophora alopecuroides, the male fern rhizome and the like have the effects of eliminating dampness, detoxifying and killing insects, and the rhubarb, the rhizoma cyperi, the cubeb and the betel nut are used as ministerial drugs for purgation, promoting qi circulation and removing retention, and the monarch drugs are used for assisting in expelling and killing the retention of insects so that the retention of insects is bitter and the retention of qi is discharged; the sweet and sour products such as pumpkin seeds and cherokee rose are used to kill parasites with astringents. The combination of the ingredients can make the worms bitter and move downwards, and the qi move outwards, the pungent and sour and astringent, so as to achieve the effects of killing parasites and removing food retention.

In the traditional Chinese medicine composition provided by the invention, the monarch drug omphalia contains omphalin, and the omphalin has the effect of repelling and killing tapeworm; agrimophol contained in the agrimony bud has the effects on the head joint, the neck joint and the body joint of the tapeworm, specifically acts on tapeworm organs, and has the synergistic effect with the omphalia; the rhizoma Dryopteris Crassirhizomatis has a paralytic effect on tapeworm bodies, so that the tapeworms cannot attach to the intestinal wall; is beneficial to the drugs with insecticidal action such as omphalia, agrimony bud, sophora alopecuroides and the like to act on tapeworm, and improves the insecticidal effective rate. The rheum officinale and the betel nut can further improve the effect of expelling and killing tapeworm, and meanwhile, the fragrant citrus fruit and the litsea cubeba have the effects of relieving pain and alleviating discomfort caused by expelling and killing the tapeworm, and the pumpkin seeds and the cherokee rose flower are used for promoting the discharge of tapeworm bodies.

The curative effect test and the prevention test result of the traditional Chinese medicine composition on the taeniasis of the laying hens show that the traditional Chinese medicine composition can effectively prevent the incidence and the mortality of the taeniasis of the healthy laying hens and effectively reduce the breeding loss; the traditional Chinese medicine composition can effectively reduce the mortality rate of the taeniasis-infected laying hens and improve the cure rate of the infected laying hens.

The traditional Chinese medicine composition has the advantages of definite curative effect, greenness, safety, no toxic or side effect, prevention and treatment for the taeniasis of the laying hens, and good market application value.

Detailed Description

Example 1

A traditional Chinese medicine composition for preventing and treating teniasis of laying hens is prepared from the following raw material medicines in parts by weight: 60 parts of omphalia lapidescens, 40 parts of agrimony bud, 25 parts of sophora alopecuroides, 30 parts of rhizoma dryopteris crassirhizomae, 40 parts of rheum officinale, 40 parts of rhizoma cyperi, 35 parts of cubeb, 30 parts of betel nut, 30 parts of pumpkin seed and 40 parts of cherokee rose flower.

The raw material medicines are taken according to the weight parts, crushed, sieved by a 60-mesh sieve, and the oversize product is crushed again until the oversize product is all sieved by the 60-mesh sieve, and all undersize products are mixed uniformly to obtain the traditional Chinese medicine composition, and the traditional Chinese medicine composition is subpackaged.

Example 2

A traditional Chinese medicine composition for preventing and treating teniasis of laying hens is prepared from the following raw material medicines in parts by weight: 70 parts of omphalia lapidescens, 30 parts of agrimony bud, 20 parts of sophora alopecuroides, 40 parts of rhizoma dryopteris crassirhizomae, 50 parts of rheum officinale, 30 parts of rhizoma cyperi, 30 parts of cubeb, 20 parts of betel nut, 40 parts of pumpkin seed and 50 parts of cherokee rose flower.

The raw material medicines are taken according to the weight parts, crushed, sieved by a 80-mesh sieve, and the oversize product is crushed again until the oversize product is all sieved by the 80-mesh sieve, and all undersize products are mixed uniformly to obtain the traditional Chinese medicine composition, and the traditional Chinese medicine composition is subpackaged.

Example 3

A traditional Chinese medicine composition for preventing and treating teniasis of laying hens is prepared from the following raw material medicines in parts by weight: 50 parts of omphalia lapidescens, 50 parts of agrimony bud, 30 parts of sophora alopecuroides, 20 parts of rhizoma dryopteris crassirhizomae, 30 parts of rheum officinale, 50 parts of rhizoma cyperi, 40 parts of cubeb, 40 parts of betel nut, 20 parts of pumpkin seed and 30 parts of cherokee rose flower.

The raw material medicines are taken according to the weight parts, crushed and sieved by a 120-mesh sieve, the oversize products are crushed again until all the oversize products are sieved by the 120-mesh sieve, all the undersize products are mixed uniformly to obtain the traditional Chinese medicine composition, and the traditional Chinese medicine composition is subpackaged.

EXAMPLE 4 toxicity testing of the Chinese medicinal composition of the present invention

1. Test sample

(1) Test subjects: 80 healthy Wistar rats with weight of 65 +/-10 g and half male and female. Purchased from experimental animals center in Henan province, and certificate number: SCXK 2018-. After purchase, the seedlings are raised for 1 week for standby. The groups were randomly divided into 4 groups, i.e., a control group, a low dose group, a medium dose group, and a high dose group, each of which contained 10 males/females.

(2) Test drugs and drug dosages: the test medication was the traditional Chinese medicine composition prepared in reference example 1 (the recommended dose is 1kg mixed with 200kg of materials, namely 0.625g of medicinal materials/Kg.bw); the dosage of the high-dosage group, the middle-dosage group and the low-dosage group is 31.25g/Kg.bw, 18.75g/Kg.bw and 6.25g/Kg.bw in turn, namely 50 times, 30 times and 10 times of the dosage to be recommended respectively; the control group was not treated with the drug.

2. Test method

(1) Test treatment

The high dose group, the medium dose group and the low dose group are respectively administered according to the administration amounts of 31.25g/Kg.bw, 18.75g/Kg.bw and 6.25g/Kg.bw, the specific operation is as follows, the three test groups respectively accurately weigh the traditional Chinese medicine composition according to the concentration, and evenly mix with normal saline, the normal saline is suitable for mixing the mixed traditional Chinese medicine composition powder into paste, and the stomach is filled for administration for 1 time/d and continuously for 30 d. During this period, the general state of the animals (mental state, diet, activity, growth, etc.) was observed and recorded daily, and the body weight was measured 1 time per week, and each index was examined after drug withdrawal.

(2) Observation and detection index

During the test period, rats were observed daily for reaction, mental state, diet, activity, hair color, etc. after administration. The weight of the rats before administration was recorded, and the weight was measured 1 time per week after administration, and the administration dose was adjusted according to the change in weight. After the experiment, the rats in each group were weighed. The blood is subjected to hematology examination (white blood cell count and classification, red blood cell count, and hemoglobin content measurement), blood biochemical index measurement (urea nitrogen, total protein, albumin, total cholesterol, creatinine, glutamic-oxalacetic transaminase, glutamic-pyruvic transaminase, etc.), organ index (liver, spleen, kidney, thymus, ovary or testis), organ pathological section examination, etc. after administration for 30 days and withdrawal for 15 days.

3. Test results

(1) Test of the Effect of the Chinese medicinal composition of the invention on the body weight of rats

During the administration period, the food intake, activity, feces and appearance behaviors of rats in each administration group and the control group are basically normal; some of the high dose groups had poor hair color. The results of the weight comparison of rats in each administration group and the control group are shown in table 1, the results show that the weights of the rats in each administration group and the control group are in a growing trend, statistical analysis shows that the weights of the rats in the administration group and the control group are not significantly different in the same period, and the influence of the traditional Chinese medicine composition on the weight of the rats is small.

TABLE 1 test results of the effect of the Chinese medicinal composition of the present invention on the body weight of rats

(2) Test of influence of traditional Chinese medicine composition on rat organ index

After 30 days of dosing, 12 rats per group were sacrificed by femoral artery exsanguination and examined dissectively. The liver, spleen, kidney, thymus, ovary or testis are picked up quickly, peeled off carefully and weighed precisely. The visceral organ index is calculated after converting the weight into 100g, in the same way, the visceral organ index is calculated after the rest rats in each group stop taking the drug for 15 days, and the statistical analysis is carried out on the visceral organ indexes of the rats in each administration group and the control group, and the result is shown in table 2.

TABLE 2 test results of the effect of the Chinese medicinal composition on the organ index of rats

(3) Test for influence of traditional Chinese medicine composition on hematological indexes of rats

Peripheral hemograms of rats in each group were measured 30 days after administration and 15 days after withdrawal, and hematological indices of rats, i.e., categories of erythrocytes, leukocytes, hemoglobin, and leukocytes in blood of rats, were evaluated, and the results are shown in table 3. The results show that the contents of erythrocytes, leukocytes, neutrophils, lymphocytes, eosinophils and large monocytes in the blood of rats of each administration group and the control group have no significant difference in the same period. The traditional Chinese medicine composition has little influence on the hematology indexes of rats.

TABLE 3 test results of the effect of the Chinese medicinal composition on the hematological index of rats

(4) Test for influence of traditional Chinese medicine composition on biochemical indexes of blood of rat

After 30 days of administration and 15 days of withdrawal, the biochemical indexes of the blood of the rats in each group are respectively detected and statistically analyzed, the results are shown in table 4, and the results show that for the biochemical indexes of the blood of each rat, no significant difference exists between the rats in each test group and the rats in the control group. The traditional Chinese medicine composition has little influence on the biochemical indexes of the blood of rats.

TABLE 4 test results of the effect of the Chinese medicinal composition on the biochemical indexes of blood of rats

(5) Histopathological examination

Histopathological examination was performed on each group of rats 30 days after dosing and 15 days after drug withdrawal. As a result, the liver, spleen, kidney, thymus, ovary or testis of rats in the high dose group, the medium dose group and the low dose group have no pathological histological change compared with the control group after 30 days of administration; after stopping taking the medicine for 15 days, no pathological histological change is observed in the liver, spleen, kidney, adrenal gland, thymus, ovary or testis of the rats in the high dose group, the medium dose group and the low dose group compared with the control group. The traditional Chinese medicine composition does not cause pathological histological changes of rats.

4. Small knot

The test results are combined to discover that the traditional Chinese medicine composition is tested according to 50, 30 and 10 times of the recommended dose in the administration period and 15 days after drug withdrawal, the indexes of the weight, the organ index, the hematology index, the blood biochemistry index, the pathological histology examination and the like of rats in each group are respectively detected after 30 days of administration and 15 days after drug withdrawal, and no obvious toxic reaction is found in each test group after 30 days of administration and 15 days after drug withdrawal of the rats. It is shown that the use of the traditional Chinese medicine composition of the invention according to the recommended dosage of 1kg of mixed material and 200kg is safe.

Example 5 drug efficacy test

1. Test materials

(1) Test object

The test site is a village Zhenhong livestock farm in Yichuan county, Luoyang, Henan province, and the test objects are Hailan brown commercial chicks bred by the livestock farm, 12000 feathers, 31 days old, chicken tapeworm symptoms with different degrees appear in large groups, indigestion of sick poultry, appetite reduction, drinking water increase, listlessness, disordered hair, dipteran drooping, two legs weakness, diarrhea (namely, the feces are rust sorghum-like), emaciation, and white worm body tablets can be seen on some feces. The sick chicken is dissected to find splenomegaly, hepatomegaly and earthy yellow, severe small intestine punctate hemorrhage accompanied with fatty degeneration ascites, intestinal tract blockage caused by polyp, yellow small nodule in pathological intestinal tract, and polyp found in intestinal wall. Taking feces, and finding tapeworm segment, and tapeworm egg and echinococcus egg by a Bordetella ferrugineus egg concentration method. Typical taeniasis infections are judged by field practicing veterinarians.

300 sick chickens which have been diagnosed are randomly selected to be used as treatment test objects, and 240 healthy chickens are randomly selected to be used as prevention test objects, and the prevention and treatment effect of the invention is tested.

(2) Feed stuff

The Beijing valley premix is used for self-preparation of complete feed.

(3) Medicine

The Chinese medicinal compositions prepared according to the embodiments 1, 2 and 3 of the invention are respectively called Chinese medicament I, Chinese medicament II and Chinese medicament III in the following, and the specification is 2.5 kg/bag.

The western medicine is praziquantel tablets produced by Dezhou Jingxin pharmaceutical industry Co., Ltd, the specification is 0.2 g/tablet, the trade name is Jingxin Taoxuening, and the production batch number is 180506.

2. Test grouping and statistical method

(1) Test for treatment

Selecting 300 sick chickens, randomly dividing into 5 groups of 60 chickens, feeding each group independently and marking one by one; 5 groups are respectively Chinese medicinal treatment group I, Chinese medicinal treatment group II, Chinese medicinal treatment group III, western medicine group, and blank group; the administration method for each group is shown in Table 5, wherein the blank group was not subjected to any administration treatment.

TABLE 5 methods of administration for each group

Group of	Number of sick chickens (only/group)	Method of administration	Time of administration
				Chinese medicine for treating group I	60	Each 1Kg of the traditional Chinese medicine I is mixed with 200Kg of the material	Continuously used for 5 days
Chinese medicine treatment group II	60	Mixing the materials 200Kg per 1Kg of traditional Chinese medicine II	Continuously used for 5 days
				Chinese medicine for treating group III	60	Mixing materials in an amount of 200Kg per 1Kg of Chinese medicine III	Continuously used for 5 days
Western medicine group	60	Praziquantel 15mg/kg body weight, used in dressing	Only the first and third day dosing was tested
				Blank group	60	——	——

After 5 days of treatment of each test group according to the administration method described in table 5, mortality and cure rate of the sick chickens in each group were observed and recorded, and the results are shown in table 6. Wherein the cure is based on the recovery of the spirit, ingestion, feces and the like of the sick chicken.

Table 6 curative effect test results of groups on teniasis of laying hens

Group of	Number of sick chickens	Incidence (%)	Number of dead chickens	Mortality (%)	Number of cured chickens	Cure rate (%)
							Chinese medicine for treating group I	60	100	0	0	60	100
Chinese medicine treatment group II	60	100	2	3.33	58	96.67
							Chinese medicine for treating group III	60	100	3	5.00	57	95.00
Western medicine group	60	100	16	26.67	44	73.33
							Blank group	60	100	27	45.00	0	0

Table 6 shows the results of the test on the curative effect of taeniasis in laying hens in each group, and the results show that the curative ratio of taeniasis in laying hens is sequentially from high to low in a traditional Chinese medicine treatment group i, a traditional Chinese medicine treatment group ii, a traditional Chinese medicine treatment group iii, a western medicine group and a blank group; the mortality rate of the sick chicken is sequentially from low to high for the Chinese medicinal treatment I group, the Chinese medicinal treatment II group, the Chinese medicinal treatment III group, the western medicinal group and the blank group. The results show that the traditional Chinese medicine composition and the western medicine praziquantel both have curative effects on the taeniasis of the laying hens, and the curative effect of the traditional Chinese medicine composition on the taeniasis of the laying hens is superior to that of the western medicine praziquantel; the traditional Chinese medicine composition is beneficial to reducing the death rate of the sick chicken and improving the cure rate of the sick chicken. In addition, compared with the traditional Chinese medicines II and III in the traditional Chinese medicine composition, the traditional Chinese medicine I in the traditional Chinese medicine composition has better curative effect on the taeniasis of the laying hens.

(2) Prevention test

Selecting 240 healthy chickens, randomly dividing into 4 groups of 60 chickens, feeding each group independently, and marking one by one; 4 groups are respectively Chinese medicinal prevention group I, Chinese medicinal prevention group II, Chinese medicinal prevention group III, and blank group; the administration method for each group is shown in Table 7, wherein the blank group was not subjected to any administration treatment.

TABLE 7 methods of administration for each group

Group of	Number of sick chickens (only/group)	Method of administration	Time of administration
				Chinese medicine for preventing group I	60	300Kg of mixing material is added for every 1Kg of traditional Chinese medicine I	Continuously used for 5 days
Chinese medicine prevention II group	60	300Kg of mixing material per 1Kg of Chinese medicine II	Continuously used for 5 days
				Chinese medicine for preventing group III	60	Mixing the materials 300Kg per 1Kg of Chinese medicine III	Continuously used for 5 days
Blank group	60	——	——

After each group of the test is treated for 5 days according to the administration method shown in the table 7, tapeworm artificial infection is carried out on healthy chickens in each group of the test, the tapeworm morbidity and mortality of the healthy chickens in each group of the test are observed and recorded, and the tapeworm prevention test result of each group of the test is shown in the table 8.

Table 8 results of experimental prevention of cestodiasis in laying hens by groups

Group of	Quantity (only)	Number of onset (only)	Incidence (%)	Death number (only)	Mortality (%)	Protection number (only)	Protective Rate (%)
								Chinese medicine for preventing group I	60	0	0	0	0	60	100
Chinese medicine prevention II group	60	0	0	0	0	60	100
								Chinese medicine for preventing group III	60	3	5.00	1	1.67	59	98.33
Blank group	60	52	86.67	23	38.33	37	61.67

Table 8 shows the results of the test on the curative effect of taeniasis in laying hens in each group, and the results show that the morbidity and mortality of healthy chickens in each group are sequentially controlled from low to high by a traditional Chinese medicine group i for prevention, a traditional Chinese medicine group ii for prevention, a traditional Chinese medicine group iii for prevention and a blank group; the protection rate of each group of healthy chickens is tested to be a traditional Chinese medicine prevention group I, a traditional Chinese medicine prevention group II, a traditional Chinese medicine prevention group III and a blank group from high to low in sequence. The result shows that compared with the blank group, the traditional Chinese medicine composition has a good effect of preventing the taeniasis infection of the laying hens, and effectively reduces the morbidity and mortality of the taeniasis infection of the healthy chickens. Moreover, compared with the traditional Chinese medicines II and III in the traditional Chinese medicine composition, the traditional Chinese medicine I in the traditional Chinese medicine composition has a better prevention effect on taeniasis infection of healthy laying hens. The method is characterized in that before the laying hens are infected with the taeniasis, the laying hens are fed with 300Kg of the traditional Chinese medicine composition in each 1Kg of the feed, so that the taeniasis morbidity and mortality of the laying hens can be effectively prevented, the protection rate of the laying hens infected with the taeniasis is improved, and the breeding loss is effectively reduced.

3. Small knot

The experiment results are combined to show that before the laying hens are infected with the taeniasis, the laying hens are fed continuously for 5 days according to the proportion of 300Kg of mixed feed to 1Kg of the traditional Chinese medicine composition, the taeniasis morbidity and mortality of the laying hens can be effectively prevented, the protection rate of the laying hens infected with the taeniasis is improved, and the breeding loss is effectively reduced. After the laying hens are infected with taeniasis and suffer from diseases, the sick laying hens are continuously fed for 5 days according to the proportion of 200Kg of mixed feed to 1Kg of the traditional Chinese medicine composition, so that the death rate of the sick laying hens can be effectively reduced, and the cure rate of the sick laying hens is improved. The traditional Chinese medicine composition has the advantages of definite curative effect, greenness, safety and prevention and treatment on the taeniasis of the laying hens.

Claims

1. The traditional Chinese medicine composition for preventing and treating teniasis of laying hens is characterized by being prepared from the following raw material medicines in parts by weight: 60 parts of omphalia lapidescens, 40 parts of agrimony bud, 25 parts of sophora alopecuroides, 30 parts of rhizoma dryopteris crassirhizomae, 40 parts of rheum officinale, 40 parts of rhizoma cyperi, 35 parts of cubeb, 30 parts of betel nut, 30 parts of pumpkin seed and 40 parts of cherokee rose flower.

2. The traditional Chinese medicine composition for preventing and treating taeniasis of laying hens according to claim 1, which is prepared by the following steps: mixing Omphalia, herba Agrimoniae bud, herba Sophorae Alopecuroidis, rhizoma Dryopteris Crassirhizomatis, radix Rumicis, rhizoma Cyperi, fructus Litseae, Arecae semen, semen Cucurbitae, and flos Rosae Laevigatae at a certain proportion, pulverizing, and sieving to obtain the Chinese medicinal composition.

3. The preparation method of the traditional Chinese medicine composition for preventing and treating taeniasis of laying hens according to claim 2, wherein the particle size of the obtained traditional Chinese medicine composition is not more than 60 meshes.