CN108619293A - A kind of composition and its preparation method and application being combined treatment grouper vibrio fluvialis disease using Chinese and Western medicine - Google Patents
A kind of composition and its preparation method and application being combined treatment grouper vibrio fluvialis disease using Chinese and Western medicine Download PDFInfo
- Publication number
- CN108619293A CN108619293A CN201810901336.3A CN201810901336A CN108619293A CN 108619293 A CN108619293 A CN 108619293A CN 201810901336 A CN201810901336 A CN 201810901336A CN 108619293 A CN108619293 A CN 108619293A
- Authority
- CN
- China
- Prior art keywords
- composition
- medicine
- disease
- grouper
- coptis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000003814 drug Substances 0.000 title claims abstract description 122
- 239000000203 mixture Substances 0.000 title claims abstract description 65
- 241000607291 Vibrio fluvialis Species 0.000 title claims abstract description 46
- 241001417495 Serranidae Species 0.000 title claims abstract description 35
- 201000010099 disease Diseases 0.000 title claims abstract description 32
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 title claims abstract description 32
- 238000002360 preparation method Methods 0.000 title claims abstract description 24
- 238000011282 treatment Methods 0.000 title claims abstract description 13
- 235000002991 Coptis groenlandica Nutrition 0.000 claims abstract description 32
- 229940079593 drug Drugs 0.000 claims abstract description 27
- SPFYMRJSYKOXGV-UHFFFAOYSA-N Baytril Chemical compound C1CN(CC)CCN1C(C(=C1)F)=CC2=C1C(=O)C(C(O)=O)=CN2C1CC1 SPFYMRJSYKOXGV-UHFFFAOYSA-N 0.000 claims abstract description 25
- 229960000740 enrofloxacin Drugs 0.000 claims abstract description 25
- 241000218202 Coptis Species 0.000 claims abstract 6
- 239000000843 powder Substances 0.000 claims description 71
- 238000000227 grinding Methods 0.000 claims description 13
- 241000894006 Bacteria Species 0.000 claims description 12
- 230000037396 body weight Effects 0.000 claims description 3
- 238000002651 drug therapy Methods 0.000 claims description 3
- 241000251468 Actinopterygii Species 0.000 abstract description 34
- 230000004083 survival effect Effects 0.000 abstract description 10
- 238000009360 aquaculture Methods 0.000 abstract description 6
- 238000004519 manufacturing process Methods 0.000 abstract description 6
- 238000011284 combination treatment Methods 0.000 abstract description 5
- 244000144974 aquaculture Species 0.000 abstract description 4
- 230000006806 disease prevention Effects 0.000 abstract description 4
- 208000010824 fish disease Diseases 0.000 abstract description 3
- 238000009395 breeding Methods 0.000 abstract description 2
- 230000001488 breeding effect Effects 0.000 abstract description 2
- 238000012360 testing method Methods 0.000 description 27
- 244000247747 Coptis groenlandica Species 0.000 description 26
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 13
- 239000000463 material Substances 0.000 description 12
- 238000000034 method Methods 0.000 description 12
- 238000002474 experimental method Methods 0.000 description 11
- 241000357444 Epinephelus coioides Species 0.000 description 9
- 150000001875 compounds Chemical class 0.000 description 9
- 230000000052 comparative effect Effects 0.000 description 8
- 238000002156 mixing Methods 0.000 description 8
- 239000000243 solution Substances 0.000 description 8
- 230000001186 cumulative effect Effects 0.000 description 6
- 239000002574 poison Substances 0.000 description 6
- 231100000614 poison Toxicity 0.000 description 6
- 239000000725 suspension Substances 0.000 description 6
- 230000000844 anti-bacterial effect Effects 0.000 description 5
- 230000005764 inhibitory process Effects 0.000 description 5
- 230000001225 therapeutic effect Effects 0.000 description 5
- 230000000694 effects Effects 0.000 description 4
- 241001465754 Metazoa Species 0.000 description 3
- 206010047400 Vibrio infections Diseases 0.000 description 3
- 230000001580 bacterial effect Effects 0.000 description 3
- 230000000857 drug effect Effects 0.000 description 3
- 239000001963 growth medium Substances 0.000 description 3
- 238000002347 injection Methods 0.000 description 3
- 239000007924 injection Substances 0.000 description 3
- 210000002784 stomach Anatomy 0.000 description 3
- 206010059866 Drug resistance Diseases 0.000 description 2
- 240000000233 Melia azedarach Species 0.000 description 2
- 240000004980 Rheum officinale Species 0.000 description 2
- 235000008081 Rheum officinale Nutrition 0.000 description 2
- 235000014220 Rhus chinensis Nutrition 0.000 description 2
- 240000003152 Rhus chinensis Species 0.000 description 2
- 241000607598 Vibrio Species 0.000 description 2
- 239000004480 active ingredient Substances 0.000 description 2
- 230000003385 bacteriostatic effect Effects 0.000 description 2
- 230000003115 biocidal effect Effects 0.000 description 2
- 229940126678 chinese medicines Drugs 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000005469 granulation Methods 0.000 description 2
- 230000003179 granulation Effects 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 238000007913 intrathecal administration Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000008194 pharmaceutical composition Substances 0.000 description 2
- 238000010298 pulverizing process Methods 0.000 description 2
- 229910001220 stainless steel Inorganic materials 0.000 description 2
- 239000010935 stainless steel Substances 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 208000024891 symptom Diseases 0.000 description 2
- 241000143060 Americamysis bahia Species 0.000 description 1
- 208000031295 Animal disease Diseases 0.000 description 1
- 241001674345 Callitropsis nootkatensis Species 0.000 description 1
- 208000004232 Enteritis Diseases 0.000 description 1
- 206010015856 Extrasystoles Diseases 0.000 description 1
- 206010021531 Impetigo Diseases 0.000 description 1
- 241000896292 Odontothrips loti Species 0.000 description 1
- 206010040047 Sepsis Diseases 0.000 description 1
- 206010053615 Thermal burn Diseases 0.000 description 1
- 210000001015 abdomen Anatomy 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 230000002508 compound effect Effects 0.000 description 1
- 238000002224 dissection Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 239000004540 pour-on Substances 0.000 description 1
- 239000000955 prescription drug Substances 0.000 description 1
- 229940126532 prescription medicine Drugs 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000003014 reinforcing effect Effects 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 208000013223 septicemia Diseases 0.000 description 1
- 235000015170 shellfish Nutrition 0.000 description 1
- 208000017520 skin disease Diseases 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 241001515965 unidentified phage Species 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/71—Ranunculaceae (Buttercup family), e.g. larkspur, hepatica, hydrastis, columbine or goldenseal
- A61K36/718—Coptis (goldthread)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
- A61K31/495—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with two or more nitrogen atoms as the only ring heteroatoms, e.g. piperazine or tetrazines
- A61K31/496—Non-condensed piperazines containing further heterocyclic rings, e.g. rifampin, thiothixene or sparfloxacin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
- A61K36/736—Prunus, e.g. plum, cherry, peach, apricot or almond
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/75—Rutaceae (Rue family)
- A61K36/756—Phellodendron, e.g. corktree
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
Landscapes
- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Epidemiology (AREA)
- Alternative & Traditional Medicine (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Communicable Diseases (AREA)
- Oncology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Organic Chemistry (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The present invention provides the composition and its preparation method and application using Chinese and Western medicine combination treatment grouper vibrio fluvialis disease, belongs to technical field of aquaculture.The composition for the treatment of grouper vibrio fluvialis disease, including the coptis, dark plum, Cortex Phellodendri and Enrofloxacin are combined using Chinese and Western medicine；The coptis, dark plum, Cortex Phellodendri and Enrofloxacin mass ratio be 0.9~1.3：0.9~1.3：0.9~1.3：0.9~1.3.Composition can largely improve drug action associated with the Chinese and Western medicine of preparation, and the survival rate than disease fish of not offeing medicine improves 85% or more.Composition compatibility science provided by the invention, at low cost, short treating period, cure rate is high, and help is provided for breeding production fish disease prevention and cure.
Description
Technical field
The invention belongs to technical field of aquaculture, and in particular to a kind of to utilize Chinese and Western medicine combination treatment grouper river arc
The composition and its preparation method and application of bacterium disease.
Background technology
Vibrio fluvialis (Vibrofluvialis) be cultivate grouper one of main pathogen, can cause a variety of fish,
The septicemia of Shrimp waste and shellfish, putrid skin disease and impetigo cause serious harm to culture fishery, are caused to aquaculture
Huge economic loss.It can also be by the propagation of marine product but the illnesss such as human infection's abdomen gulf so as to cause the acute stomach of the mankind
Enteritis, it follows that vibrio fluvialis belongs to a kind of pathogenic bacteria that people contaminates altogether with aquiculture animal therefore no matter in aquatic products field
Or all oneself becomes one of the hot issue studied both at home and abroad to other field vibrios.
Antibiotic is the main means of current bacteriosis of aquatic livestock treatment, as antibiotic is treating bacillary disease
Extensive use in disease generates bacterial drug resistance, leads to the shortcomings of medicament residue.And grouper itself resistance increasingly reduces, disease
Evil problem is on the rise, and big loss is brought to Grouper cultivating industry.Grouper vibrio fluvialis disease has resulted in serious commercial damage
It loses, it is mainly Western medicine that fish disease is treated in breeding production, and germ has generated drug resistance, and therapeutic effect is bad.In research and development
Aquatic Animal Disease Prevention fish disease is significant.China's traditional Chinese medicine can also enhance aquiculture animal and exempt from addition to it can overcome aforesaid drawbacks
Epidemic disease power, experiment show that Chinese medicine and Chinese medicine compound prescription have preferable extracorporeal extracorporeal suppression to bacterium.The retrieval coptis, dark plum, Cortex Phellodendri have
A small amount of patent, but all it is folk prescription medication, in the formula of disease prevention and cure for reinforcing animal etc., and obtain certain curative effect.
However it is to belong to the profit of vibrio fluvialis using the application of vibrio fluvialis bacteriophage sterilization etc. mostly in the prior art
With the patent of no any vibrio fluvialis disease prevention does not have this patent grouper vibrio fluvialis disease to treat patent family, not related yet
In the report of grouper vibrio fluvialis disease therapeutic scheme.
Invention content
In view of this, being combined treatment grouper vibrio fluvialis disease using Chinese and Western medicine the purpose of the present invention is to provide a kind of
Composition and its preparation method and application, the composition have stronger drug effect to disease caused by vibrio fluvialis.
In order to achieve the above-mentioned object of the invention, the present invention provides following technical scheme：
The present invention provides the composition using Chinese and Western medicine combination treatment grouper vibrio fluvialis disease, including the coptis, dark plum, Huang
Cypress and Enrofloxacin；The coptis, dark plum, Cortex Phellodendri and Enrofloxacin mass ratio be 0.9~1.3：0.9~1.3：0.9~
1.3：0.9~1.3.
Preferably, the mass ratio of the coptis, dark plum, Cortex Phellodendri and Enrofloxacin is 1：1：1：1.
The present invention provides the preparation methods of the composition, include the following steps：
1) coptis, dark plum and Cortex Phellodendri are crushed, obtains herb powder；
2) herb powder is subjected to ultramicro grinding, obtains ultramicro medicine powder；
3) ultramicro medicine powder is toasted to 24~48h under the conditions of 60~70 DEG C, obtains traditional Chinese medicine fine powder；
4) traditional Chinese medicine fine powder and Enrofloxacin are mixed, obtains composition.
Preferably, the grain size of herb powder is 140~160 μm in step 1)；
The grain size of ultramicro medicine powder is 5~10 μm in the step 2).
Preferably, the temperature toasted in the step 3) is 65 DEG C, and the time of the baking is 36h.
The present invention provides the compositions to prepare the application in treating grouper vibrio fluvialis medicine.
Preferably, the quality of the composition accounts for the 1.8~2.4% of drug quality.
Preferably, the dosage of the composition is the 2%~3% of grouper fish body weight.
Preferably, the administration time of the drug therapy grouper vibrio fluvialis disease is 30~45d.
The present invention provides the composition using Chinese and Western medicine combination treatment grouper vibrio fluvialis disease, including the coptis, dark plum, Huang
Cypress and Enrofloxacin；The coptis, dark plum, Cortex Phellodendri and Enrofloxacin mass ratio be 0.9~1.3：0.9~1.3：0.9~
1.3：0.9~1.3, compound medicine has synergistic function, can it is larger improve folk prescription medicine drug effect, reduce the dosage of each medicine, simultaneously
Composition associated with the Chinese and Western medicine of preparation can largely improve the drug effect for the treatment of grouper vibrio fluvialis disease, than disease fish of not offeing medicine
Survival rate improve 85% or more.
Composition provided by the invention compounds the formula to be formed treatment grouper using three Chinese medicines and Enrofloxacin simultaneously
Vibrio fluvialis disease, compared with folk prescription Chinese medicine therapeutic effect, has the advantages that treatment time is shorter, better efficacy, especially than pure west
Medicine medicine residual is few.
The present invention provides the preparation methods of the composition, and the traditional Chinese medicinal components coptis, dark plum, Cortex Phellodendri are passed through powder successively
Broken, ultramicro grinding and baking, obtained traditional Chinese medicine fine powder and Enrofloxacin mixing, are prepared composition.The preparation method has
It is fully discharged conducive to traditional Chinese medicinal components pharmaceutical active ingredient, improves efficacy of drugs, while the method preparation process is simple, used
Mechanical processing production, it is reproducible, it is advantageously implemented mass production.
The present invention provides the compositions to prepare the application in treating grouper vibrio fluvialis medicine.The combination
Object can effectively inhibit the growth of vibrio fluvialis, to realize that the drug of preparation has the mesh of effectively treatment grouper vibrio fluvialis disease
's.
Specific implementation mode
The present invention provide Chinese and Western medicine combination treatment grouper vibrio fluvialis disease composition, including the coptis, dark plum, Cortex Phellodendri and
Enrofloxacin；The coptis, dark plum, Cortex Phellodendri and Enrofloxacin mass ratio be 0.9~1.3：0.9~1.3：0.9~1.3：0.9
~1.3, preferably 1：1：1：1.
The present invention is not particularly limited the source of the coptis, dark plum, Cortex Phellodendri, and use is well known to those skilled in the art
The coptis, dark plum, Cortex Phellodendri.In embodiments of the present invention, the coptis, dark plum and Cortex Phellodendri are purchased from Xiamen Lian Futang pharmacies.
The present invention is not particularly limited the source of the Enrofloxacin, using Enrofloxacin known in the art.In this hair
In bright embodiment, the Enrofloxacin is purchased from Xinghua Hengwei Biotechnology Co., ltd..
The present invention provides the preparation methods of the composition, include the following steps：
1) coptis, dark plum and Cortex Phellodendri are crushed, obtains herb powder；
2) herb powder is subjected to ultramicro grinding, obtains ultramicro medicine powder；
3) ultramicro medicine powder is toasted to 24~48h, obtained traditional Chinese medicine fine powder under the conditions of 60~70 DEG C；
4) traditional Chinese medicine fine powder and Enrofloxacin are mixed, obtains composition.
The present invention crushes the coptis, dark plum and Cortex Phellodendri, obtains herb powder.The grain size of herb powder after crushing is preferably
140~160 μm.More preferably 150 μm.Include that the coptis, dark plum and Cortex Phellodendri crush or respectively by the coptis, dark plum when the crushing
It is crushed again according to said ratio mixing with Cortex Phellodendri.The present invention is not particularly limited the method for the crushing, using this field skill
Breaking method known to art personnel.In embodiments of the present invention, the crushing crushing that instrument is model FW177
Machine is purchased from Tianjin Stettlen Instrument Ltd..
After obtaining herb powder, the herb powder is carried out ultramicro grinding by the present invention, obtains ultramicro medicine powder.
In the present invention, preferably the herb powder is toasted before the ultramicro grinding.The temperature of the baking is excellent
It is selected as 60~70 DEG C, more preferably 65 DEG C.The time of the baking is preferably 24~48h, more preferably 36h.Baking makes ultra micro
The water content of medicinal powder reduces, and makes the requirement for meeting ultramicro grinding.
In the present invention, the grain size of the ultramicro medicine powder is preferably 5~10 μm, more preferably 6~9 μm, most preferably 8 μ
m.The present invention is not particularly limited the method for the ultramicro grinding, and the method using ultramicro grinding known in the art is
It can.In embodiments of the present invention, the ultramicro grinding vibration pulverizing mill clearly of three that instrument is model SQW-60, is purchased from Shandong
Three clear stainless steel equipment Co., Ltds.The ultramicro grinding is conducive to Chinese medicine pharmaceutical active ingredient and fully releases.
After obtaining ultramicro medicine powder, the ultramicro medicine powder is toasted 24~48h by the present invention under the conditions of 60~70 DEG C, in obtaining
Drug composition.In the present invention, it is 65 DEG C that the temperature of the baking is excellent, and the excellent time of the baking is 36h.Baking makes ultra micro medicine
The water content of powder reduces.
After obtaining traditional Chinese medicine fine powder, the present invention mixes the traditional Chinese medicine fine powder and Enrofloxacin according to above-mentioned mass ratio, obtains
Composition.The present invention is not particularly limited the mode of the mixing, using hybrid plan well-known to those skilled in the art
.
The present invention also provides application of the composition in the drug for preparing treatment grouper vibrio fluvialis disease.
In the present invention, three Chinese medicine compositions include the following steps the bacteriostatic experiment of grouper vibrio fluvialis：It is described
Chinese medicine composition is formulated as solution, by obtained Chinese medicine composition solution to 0.1mL bacterium amounts concentration 108The vibrio fluvialis of CFU/mL
It instills tablet, smoothen, observe antibacterial situation.As a result, it has been found that it is pole that the effective concentration of Chinese medicine composition solution, which is 200~240mg/L,
It is sensitive.The effective concentration of Chinese medicine composition solution is more preferably 210~230mg/L, most preferably 220mg/L.
In the present invention, the drug further includes auxiliary material.The auxiliary material includes grouper basal feed.The system of the drug
Preparation Method is to mix composition prepared by said program with auxiliary material, pelletizes, obtains drug.
In the present invention, the quality of the composition preferably accounts for the 1.8%~2.4% of drug gross mass, more preferably
2.0%.The feeding amount of the composition is preferably the 2%~3% of quality of fishes, and more preferably 2.5%.
In the present invention, the preparation method of the drug preferably includes following steps：Feed fine powder is made in feed, it will be upper
The Chinese medicine composition for stating scheme preparation is mixed with feed fine powder, is pelletized, is obtained drug.The present invention does not have the method for the mixing
It is specifically limited, using mixed method known in the art.The present invention is not particularly limited the method for the granulation, adopts
With method of granulating known in the art.
In the present invention, the administration time of the drug therapy grouper vibrio fluvialis disease is preferably 30~45d.
Grouper vibrio fluvialis disease is treated to a kind of be combined using Chinese and Western medicine provided by the invention with reference to embodiment
Composition and its preparation method and application is described in detail, but they cannot be interpreted as to the scope of the present invention
It limits.
Embodiment 1
From the Xiamen Lian Futang pharmacies purchase coptis, dark plum, Cortex Phellodendri；Medicinal medicinal herb grinder crushes (FW177, Tianjin by
Stettlen Instrument Ltd. of city) be crushed to about 150 μm, be then placed in micronizer (three clear vibration pulverizing mills, SQW-60,
Sanqing Stainless Steel Apparatus Co., Ltd., Shandong) in further crush, until grain diameter size be 5~10 μm, collect ultra micro medicine
Powder is spare.Three kinds of ultramicro medicine powders are toasted into 48h under the conditions of 60 DEG C respectively, obtain traditional Chinese medicine fine powder；It weighs obtained above
Traditional Chinese medicine fine powder, coptis fine powder 0.9kg, dark plum fine powder 1.3kg, Cortex Phellodendri fine powder 0.9kg, mixing obtain Chinese medicine composition.
Embodiment 2
The Chinese medicine of Cortex Phellodendri, the coptis, dark plum is crushed to 160 μm, obtains three kinds of herb powders；By three kinds of herb powders
Ultramicro grinding is carried out respectively to 10 μm of grain size, obtains three kinds of ultramicro medicine powders；By three kinds of ultramicro medicine powders respectively in 70 DEG C of conditions
Lower baking for 24 hours, obtains traditional Chinese medicine fine powder；Traditional Chinese medicine fine powder obtained above is weighed, coptis fine powder 1.2kg, dark plum fine powder 0.9kg are yellow
Cypress fine powder 1.0kg, mixing, obtains Chinese medicine composition.
Embodiment 3
The Chinese medicine of Cortex Phellodendri, the coptis, dark plum is crushed to 150 μm, obtains three kinds of herb powders；By three kinds of herb powders
Ultramicro grinding is carried out respectively to 8 μm of grain size, obtains three kinds of ultramicro medicine powders；By three kinds of ultramicro medicine powders respectively under the conditions of 65 DEG C
36h is toasted, traditional Chinese medicine fine powder is obtained；Weigh traditional Chinese medicine fine powder obtained above, coptis fine powder 1.0kg, dark plum fine powder 1.0kg, Cortex Phellodendri
Fine powder 1.0kg, mixing, obtains Chinese medicine composition.
Comparative example 1
Coptis traditional Chinese medicine fine powder 1.0kg prepared by embodiment 3 is weighed, folk prescription drug is obtained.
Comparative example 2
Dark plum fine powder 2.0kg and Cortex Phellodendri fine powder 2.0kg prepared by embodiment 3 is weighed, compound bigeminy Chinese medicine composition is obtained.
Comparative example 3
Ke Zi is bought from Tianjiang Pharmaceutical Industry Co., Ltd., Jiangyin, dark plum traditional Chinese medicine fine powder is prepared according to the method for embodiment 3,
Ke Zi traditional Chinese medicine fine powder 1.5kg are weighed, the Cortex Phellodendri fine powder 1.5kg and dark plum fine powder 1.5kg prepared with embodiment 3 is mixed, and is answered
Fang Sanlian Chinese medicine compositions.
Comparative example 4
By 1 × 108200 μ L bacteria suspensions of CFU/mL vibrio fluvialis are spread evenly across on LB culture medium flat plates, are put into En Nuosha
Star drug sensitive test paper (purchased from Hangzhou day and microorganism reagent Co., Ltd), judges result according to the newest standards of CLSI M100.It surveys
Antibacterial circle diameter is measured, judges the sensibility of vibrio fluvialis.
Embodiment 4~6
Composition prepared by embodiment 1 is mixed with water, and water proof heat, be prepared into solution be equivalent to crude drug quality it is dense
Degree is 200mg/ml, 220mg/ml and 240mg/ml.Composition prepared by embodiment 2 and 3 is equally prepared into according to the method described above
The drug solution of 200mg/ml, 220mg/ml and 240mg/ml.
Function and effect of the drug to bacterium are studied using Odontothrips loti, the LB poured into tablet after 10~12mL sterilizings is solid
Body culture medium after to be solidified, be gently put into 4 Oxford cups in each culture dish moderate distance with tweezers and immerse in culture medium, take
The LB culture mediums (temperature is advisable with non-scald on hand) of 12~15mL are uniformly mixed and pour on tablet, and after to be solidified, Oxford cup is removed,
Vibrio fluvialis is diluted to 1.0x108~3.0x108CFU/mL draws 0.1mL bacteria suspensions with liquid-transfering gun, tablet is instilled, with coating
Device smoothens, and the sterile water that 200 μ L are added in 1 hole with liquid-transfering gun is control, and above-mentioned 3 are added in another 3 Kong Zhongyong liquid-transfering guns
The 200 μ L of liquid of kind various concentration, each test group and control group are respectively provided with 3 repeating groups.It is placed under the conditions of 26~30 DEG C, it is permanent
Temperature 18~36h of culture, observes inhibition zone, SupcreG1 bacterium colonies counting/screening/inhibition zone measurement combined instrument (fast number in Hangzhou is used in combination
Science and Technology Ltd.) inhibition zone size is measured, each test 3 repetitions.Average value is calculated, comparative study is carried out, according to antibacterial
Loop diameter size judges the power of its bacteriostasis.Inhibition zone the results are shown in Table 1.
The antibacterial situation of composition various concentration solution prepared by 1 Examples 1 to 3 of table
Comparative example 5~8
The Chinese Medicines for preparing comparative example 1~4 according to the method for embodiment 4~6 carry out bacteriostatic experiment, observe inhibition zone
And bacteriostasis.It the results are shown in Table 2.
The antibacterial situation of composition various concentration solution prepared by 2 comparative example 1~4 of table
Embodiment 7~11
Chinese medicine composition prepared by Examples 1 to 3 is respectively with grouper basal feed according to 1.8~2.4：98.2~
97.6 mass ratio mixing, granulation obtain 3 groups of drugs.
Grouper is tested per tail with 103The vibrio fluvialis viable bacteria bacteria suspension of CFU/g is by way of intrathecal to tested
Fish carries out attacking poison, vibrio fluvialis living bacterial liquid 0.2mL.It attacks after poison and all feeds basal feed for every group, feed 40d, obtain suffering from river
The grouper of vibrio infection.Each aquaculture pond launches the grouper that 20 tails suffer from vibrio fluvialis infection.
Test group 1~3 feeds 3 groups of drugs of above-mentioned preparation, and specific feeding relationship is shown in Table 3, and scale of feeding is comparable to implement
The dosage of composition prepared by example 1~3 is the 2%~3% of fish body weight, and the number fed daily is 2 times, feeds 40d altogether.It waits for
Residual bait is sucked out in fish on a full stomach.Day changes that water is primary, and quantity of exchanged water is the 1/3 of total volume.It is (normal to feed basis that control group I is also set up simultaneously
Feed, healthy fish) and control group II (normally feeding basal feed, vibrio fluvialis attacks malicious fish), each test group and control group are all provided with
Set 3 repeating groups.It keeps observing and recording survival condition (observation 40d) daily, records disease symptom and death condition, solve dissect
Dead fish is looked into, cumulative mortality (Cumulative mortalityrate) is calculated.
Fish continues to feed with upper type if there is survival in each group of experiment, until fish is all dead.Statistics fish 50%,
100% death time.
3 groups of drugs of 3 test group of table, 1~3 above-mentioned preparation and the cumulative mortality of control group
Embodiment 12
It weighs in being prepared in Enrofloxacin (being purchased from Xinghua Hengwei Biotechnology Co., ltd.) 1.3kg, with embodiment 1
Drug composition mixes, and obtains Chinese and Western medicine compound medicine composition.
Embodiment 13
It weighs in being prepared in Enrofloxacin (being purchased from Xinghua Hengwei Biotechnology Co., ltd.) 0.9kg, with embodiment 2
Drug composition mixes, and obtains Chinese and Western medicine compound medicine composition.
Embodiment 14
It weighs in being prepared in Enrofloxacin (being purchased from Xinghua Hengwei Biotechnology Co., ltd.) 1.0kg, with embodiment 3
Drug composition mixes, and obtains Chinese and Western medicine compound medicine composition.
Embodiment 15~17
1, artificial liver support:Before experiment starts, experiment Epinephelus coioides injection vibrio fluvialis is carried out attacking malicious preliminary experiment,
80% or more occurs illness but dead concentration is not caused to be set to experiment bacterial concentration.Epinephelus coioides are purchased from Zhangpu city of Fujian Province
Certain farm, body are grown (11 ± 2) cm, are temporarily supported in the circulating water culture system of 1t water bodys.For testing after temporarily supporting 1 week.Weight,
The comparable healthy Epinephelus coioides of Individual Size are randomly divided into experimental group and control group, every group of each 20 tail, are followed respectively at 18 DEG C
Ring water cultivating system endoadaptation is raised one week.With 103The vibrio fluvialis bacteria suspension of CFU/g by way of intrathecal to by
Examination fish carries out attacking poison.Control group Epinephelus coioides are then injected with PBS buffer solution.Then 4 groups of fishes are transferred in raising water body respectively
It is fed.Experiment sets control group I (feeding material, healthy fish), control group II (feeding material, vibrio fluvialis attack malicious fish), test group I
(feeding material plus the Chinese and Western medicine of the preparation of embodiment 12 are combined composition, and vibrio fluvialis attacks malicious fish), test group II (feeding material plus implementation
Chinese and Western medicine prepared by example 13 is combined composition, and vibrio fluvialis attacks malicious fish), test group III is (during feeding material adds embodiment 14 to prepare
Western medicine is combined composition, and vibrio fluvialis attacks malicious fish), therapeutic effect comparison separately is carried out with common drug in production, if control group III
(feeding material plus 2.2% rheum officinale Chinese gall dissipate, river for (feeding material plus 2.2% plate Huang dissipate, and vibrio fluvialis attacks malicious fish) and control group IV
Vibrios attacks malicious fish), each test group and control group are respectively provided with 3 repeating groups.After on-test, daily in 8:00、17:00 feeds
2 times, feeding amount be the quality of pharmaceutical composition is equivalent to account for the 2%~3% of quality of fishes, and according to grow, situation of ingesting is made
Adjustment.Wait for that residual bait is sucked out in fish on a full stomach.Day changes that water is primary, and quantity of exchanged water is the 1/3 of total volume.Experimental basis feed is carried by company
For.Addition Chinese herbal formula, Chinese and Western medicine are combined preparation in test feed difference, are not added in control group feed.
It keeps observing and recording survival condition (observation 40d) daily, records disease symptom and death condition, dissection checks dead
Fish is died, cumulative mortality (Cumulative mortalityrate) is calculated.Each group of fish continues to if there is survival with top
Formula is fed, until fish is all dead.
RCM=[(N0-Nt)/N0] × 100% formula a
In formula a:RCM is cumulative mortality (%)；N0 is experiment just fish number；Nt is off-test fish number；Survey the death rate (just
Beginning fish survival rate, half fish death time, whole fish death times).
2, the survival rate result of pharmaceutical chemistry group and control group fish
The control group and death condition of test group is as shown in table 4 after viable bacteria artificial challenge's Epinephelus coioides.
For challenge test result after challenge test 5 days, there is death, experiment card successively in each group Epinephelus coioides experimental group
Bright, the death for attacking fish in 40d after poison tends towards stability, therefore the death toll of each group in 40d after poison is attacked in this test data sheet, is attacking poison the 1st
It and the 2nd day each test group fish food ration significantly reduce, sick fish The dead quantity：Control group II>Control group IV>Control group III>Examination
Test group II>Test group III>Test group I>Control group I.
When off-test, control group I (blank control group, health fish feed material) health is survived, and survival rate is
100.00%, and activity and normal, the illness being visible by naked eyes of ingesting.
There is dead 1 tail in the 6d after bacteria suspension injection of control group II, and 6~10d is peak mortality phase, dead 11 tail, 40d
It is living：0 tail.
Test group I, test group II and test group III, there is dead 1 tail in 4~9d after bacteria suspension injection, and 42~50d is death
Peak period.And it is respectively 85.00%, 95% to use common medicine treatment group control group III, the IV 40d death rates of control group, and it is dead
It dies rate and is higher than I~III medication of test group of the present invention.
The Epinephelus coioides resistance that pharmaceutical composition group associated with Chinese and Western medicine is added in feed is apparently higher than control group.It controls
Therapeutic effect is dissipated better than the common medicine plate Huang of production, rheum officinale Chinese gall dissipates.
The survival rate result of the Epinephelus coioides of 4 pharmaceutical chemistry group of table and control group
By above-described embodiment it is found that feeding material plus the coptis, dark plum and Cortex Phellodendri Chinese medicine compound prescription can preferably treat Epinephelus coioides
Vibrio fluvialis disease.And the coptis, dark plum, Cortex Phellodendri and Enrofloxacin composition Chinese and Western medicine compound effect ratio Chinese medicine compound prescription of curing the disease more have treatment
Effect.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (9)
1. a kind of composition being combined treatment grouper vibrio fluvialis disease using Chinese and Western medicine, which is characterized in that including the coptis, crow
Plum, Cortex Phellodendri and Enrofloxacin；The coptis, dark plum, Cortex Phellodendri and Enrofloxacin mass ratio be 0.9~1.3：0.9~1.3：0.9
~1.3：0.9~1.3.
2. composition according to claim 1, which is characterized in that the coptis, dark plum, Cortex Phellodendri and Enrofloxacin quality
Than being 1：1：1：1.
3. the preparation method of composition described in claims 1 or 2, which is characterized in that include the following steps：
1) coptis, dark plum and Cortex Phellodendri are crushed, obtains herb powder；
2) herb powder is subjected to ultramicro grinding, obtains ultramicro medicine powder；
3) ultramicro medicine powder is toasted to 24~48h under the conditions of 60~70 DEG C, obtains traditional Chinese medicine fine powder；
4) traditional Chinese medicine fine powder and Enrofloxacin are mixed, obtains composition.
4. preparation method according to claim 3, which is characterized in that the grain size of herb powder is 140~160 μ in step 1)
m；
The grain size of ultramicro medicine powder is 5~10 μm in the step 2).
5. according to the preparation method of claim 3 or 4, which is characterized in that the temperature toasted in the step 3) is 65 DEG C, institute
The time for stating baking is 36h.
6. composition described in claims 1 or 2 is preparing the application in treating grouper vibrio fluvialis medicine.
7. application according to claim 6, which is characterized in that the quality of the composition account for drug quality 1.8~
2.4%.
8. application according to claim 6, which is characterized in that the dosage of the composition is grouper fish body weight
2%~3%.
9. according to the application described in claim 6~8 any one, which is characterized in that drug therapy grouper river arc
The administration time of bacterium disease is 30~45d.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810901336.3A CN108619293A (en) | 2018-08-09 | 2018-08-09 | A kind of composition and its preparation method and application being combined treatment grouper vibrio fluvialis disease using Chinese and Western medicine |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810901336.3A CN108619293A (en) | 2018-08-09 | 2018-08-09 | A kind of composition and its preparation method and application being combined treatment grouper vibrio fluvialis disease using Chinese and Western medicine |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108619293A true CN108619293A (en) | 2018-10-09 |
Family
ID=63708900
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810901336.3A Pending CN108619293A (en) | 2018-08-09 | 2018-08-09 | A kind of composition and its preparation method and application being combined treatment grouper vibrio fluvialis disease using Chinese and Western medicine |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108619293A (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103239544A (en) * | 2013-05-29 | 2013-08-14 | 北京大北农动物保健科技有限责任公司 | Fishery Chinese and western compound medicine composition as well as premix compound, batch mixture and application thereof |
| CN104721337A (en) * | 2013-12-18 | 2015-06-24 | 洛阳惠中兽药有限公司 | Traditional Chinese medicine composition, preparation method and application thereof |
| CN105168667A (en) * | 2015-10-27 | 2015-12-23 | 上海海洋大学 | Application of Chinese herbal medicament to delaying of bacterial drug resistance |
| US20180185430A1 (en) * | 2016-12-30 | 2018-07-05 | Yujia Zhu | Herbal Composition for Shrimp Disease |
-
2018
- 2018-08-09 CN CN201810901336.3A patent/CN108619293A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103239544A (en) * | 2013-05-29 | 2013-08-14 | 北京大北农动物保健科技有限责任公司 | Fishery Chinese and western compound medicine composition as well as premix compound, batch mixture and application thereof |
| CN104721337A (en) * | 2013-12-18 | 2015-06-24 | 洛阳惠中兽药有限公司 | Traditional Chinese medicine composition, preparation method and application thereof |
| CN105168667A (en) * | 2015-10-27 | 2015-12-23 | 上海海洋大学 | Application of Chinese herbal medicament to delaying of bacterial drug resistance |
| US20180185430A1 (en) * | 2016-12-30 | 2018-07-05 | Yujia Zhu | Herbal Composition for Shrimp Disease |
Non-Patent Citations (1)
| Title |
|---|
| 贾春红: "《抗致病性弧菌及希瓦氏菌有效中药与方剂的筛选、抗菌机理研究》", 《中国优秀硕士学位论文全文数据库 农业科技辑》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN100425258C (en) | Compound Chinese medicine composition and its prepn and use | |
| CN102389499B (en) | Traditional Chinese medicinal extract used for controlling viral diseases in aquiculture and preparation method thereof | |
| CN102038781B (en) | Pharmaceutical composition for inhibiting bacteria and preparation method and application thereof | |
| CN101829211B (en) | Fish compound traditional Chinese medicinal preparation and preparation method thereof | |
| CN104352617A (en) | Sanhuang supper micropowder as well as preparing method and application thereof | |
| CN101721489B (en) | Plant extract formula for inhibiting aeromonas hydrophila | |
| KR100918628B1 (en) | Composition for prevention or treatment of liver toxicity comprising fungus-fermented Ssangwhatang | |
| CN112375708B (en) | Preparation for resisting white spot syndrome virus and Octopus iridovirus 1 and preparation method thereof | |
| CN102240323A (en) | Traditional Chinese medicine for controlling aeromonas hydrophila disease of aquatic animals and utilizing method thereof | |
| CN111676197B (en) | Vibrio harveyi phage, phage composition and application thereof | |
| CN108815279A (en) | It is a kind of for treating the Chinese medicine composition and its preparation method and application of grouper vibrio fluvialis disease | |
| CN108619247A (en) | A kind of composition and its preparation method and application being combined treatment grouper vibrio alginolyticus disease using Chinese and Western medicine | |
| CN108815241A (en) | It is a kind of for treating the Chinese medicine composition and its preparation method and application of grouper vibrio alginolyticus disease | |
| CN108619293A (en) | A kind of composition and its preparation method and application being combined treatment grouper vibrio fluvialis disease using Chinese and Western medicine | |
| CN106943389B (en) | It is a kind of prevent and treat fish-egg saprolegniasis preparation and its application | |
| CN102048059B (en) | Feed additive used for preventing and treating vibrio gill-rot disease of aquatic animals and preparation method thereof | |
| CN115887555A (en) | Traditional Chinese medicine composition for inhibiting aspergillus flavus | |
| CN108635446A (en) | It is a kind of to be combined the composition and its preparation method and application that treatment grouper deforms pseudomonad disease using Chinese and Western medicine | |
| CN102218078B (en) | Rifaximin suspension containing montmorillonite and preparation method thereof | |
| CN102125178B (en) | Feed additive for preventing and treating virus caused death of aquatic livestock and preparation method thereof | |
| CN108815280A (en) | A kind of Chinese medicine composition and its preparation method and application deforming pseudomonad disease for treating grouper | |
| CN105250387A (en) | Antibacterial Chinese herbal medicine compound for fishes as well as preparation method and application of antibacterial Chinese herbal medicine compound | |
| CN108815281A (en) | A kind of aquatic products Sanhuang San and preparation method thereof | |
| CN1284372A (en) | 'Sanhuangqinbao' powder-medicine for chicken | |
| CN108850704A (en) | Chinese herbal feed additive and its preparation method and application for Penaeus Vannmei |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20181009 |