CN108815241A - It is a kind of for treating the Chinese medicine composition and its preparation method and application of grouper vibrio alginolyticus disease - Google Patents
It is a kind of for treating the Chinese medicine composition and its preparation method and application of grouper vibrio alginolyticus disease Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/484—Glycyrrhiza (licorice)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/65—Paeoniaceae (Peony family), e.g. Chinese peony
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/71—Ranunculaceae (Buttercup family), e.g. larkspur, hepatica, hydrastis, columbine or goldenseal
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/15—Preparation or pretreatment of starting material involving mechanical treatment, e.g. chopping up, cutting or grinding
Abstract
The present invention provides a kind of for treating the Chinese medicine composition and its preparation method and application of grouper vibrio alginolyticus disease, belongs to technical field of aquaculture.It is a kind of for treating the Chinese medicine composition of grouper vibrio alginolyticus disease, including myrobalan, Radix Paeoniae Alba, Radix Glycyrrhizae；The mass ratio of the myrobalan, Radix Paeoniae Alba and Radix Glycyrrhizae are 0.9~1.3：0.9~1.3：0.9~1.3.The Chinese medicine composition of preparation can largely improve drug action, and the survival rate than disease fish of not offeing medicine improves 70% or more.Chinese medicine composition compatibility science provided by the invention, at low cost, short treating period, cure rate is high, provides help for breeding production fish disease prevention and cure.
Description
Technical field
It is specific a kind of for treating the Chinese traditional medicine composition of grouper vibrio alginolyticus disease the invention belongs to technical field of aquaculture
Object and its preparation method and application.
Background technique
There is not understatement in coastal in south China and Taiwan to vibrio alginolyticus (Vibrio alginolyticus) in recent years
Road, is the pathogenic bacteria for seriously endangering the marine cultured animals such as fish, shrimp, shellfish, and fish disease caused by vibrio alginolyticus has morbidity rapidly, extremely
The features such as rate is high, popular wide is died, the sustainable health development of culture fishery is threatened, prevention and treatment is always the emphasis of research.
Antibiotic is the main means of current bacteriosis of aquatic livestock treatment, as antibiotic is treating bacillary disease
It is widely applied in disease, generates bacterial drug resistance, leads to the disadvantages of medicament residue.And grouper itself resistance increasingly reduces, disease
Evil problem is on the rise, and big loss is brought to Grouper cultivating industry.Grouper vibrio alginolyticus disease has resulted in serious commercial damage
It loses, it is mainly Western medicine that fish disease is treated in breeding production, and germ has generated drug resistance, and therapeutic effect is bad.In research and development
Aquatic Animal Disease Prevention fish disease is significant.China's traditional Chinese medicine can also enhance aquiculture animal and exempt from addition to it can overcome aforesaid drawbacks
Epidemic disease power, experiment show that Chinese medicine and Chinese medicine compound prescription have preferable extracorporeal extracorporeal suppression to bacterium.Retrieval myrobalan, Radix Paeoniae Alba, Radix Glycyrrhizae have
A small amount of patent, but all it is folk prescription medication, in the formula of disease prevention and cure for reinforcing animal etc., and obtain certain curative effect.
It however is to belong to the benefit of vibrio alginolyticus using the application of vibrio alginolyticus bacteriophage sterilization etc. mostly in the prior art
With the patent of no any vibrio alginolyticus disease prevention and treatment does not have this patent grouper vibrio alginolyticus disease to treat patent family, not related yet
In the report of grouper vibrio alginolyticus disease therapeutic scheme.
Summary of the invention
In view of this, the purpose of the present invention is to provide a kind of for treating the Chinese medicine composition of grouper vibrio alginolyticus disease
And its preparation method and application, the Chinese medicine composition has stronger drug effect to disease caused by vibrio alginolyticus.
In order to achieve the above-mentioned object of the invention, the present invention provides following technical scheme：
The present invention provides the Chinese medicine composition for the treatment of grouper vibrio alginolyticus disease, including myrobalan, Radix Paeoniae Alba and Radix Glycyrrhizae；It is described to scold
Son, Radix Paeoniae Alba and the mass ratio of Radix Glycyrrhizae are 0.9~1.3：0.9~1.3：0.9~1.3.
Preferably, the mass ratio of the myrobalan, Radix Paeoniae Alba and Radix Glycyrrhizae is 1：1：1.
The present invention provides the preparation methods of the Chinese medicine composition, include the following steps：
1) myrobalan, Radix Paeoniae Alba and Radix Glycyrrhizae are crushed, obtains herb powder；
2) herb powder is pulverized, obtains ultramicro medicine powder；
3) ultramicro medicine powder is toasted to 24~48h under the conditions of 60~70 DEG C, obtains Chinese medicine composition.
Preferably, the partial size of herb powder is 140~160 μm in step 1)；
The partial size of ultramicro medicine powder is 5~10 μm in the step 2).
Preferably, the temperature toasted in the step 3) is 65 DEG C, and the time of the baking is 36h.
The present invention provides application of the Chinese medicine composition in the drug of preparation treatment grouper vibrio alginolyticus disease.
Preferably, effective Mlc of the Chinese medicine composition is 200~240mg/L.
Preferably, the quality of described pharmaceutical composition accounts for the 1.8~2.4% of drug gross mass.
Preferably, the administration time of the drug therapy grouper vibrio alginolyticus disease is 30~45d.
The present invention provide it is a kind of for treating the Chinese medicine composition of grouper vibrio alginolyticus disease, by myrobalan, Radix Paeoniae Alba and Radix Glycyrrhizae
It is 0.9~1.3 according to mass ratio：0.9~1.3：0.9~1.3 ratio combination, compound medicine have synergistic function, can be larger
The drug effect for improving folk prescription medicine reduces the dosage of each medicine, while the Chinese medicine composition prepared can largely improve treatment grouper
The drug effect of vibrio alginolyticus disease, the survival rate than disease fish of not offeing medicine improve 70% or more.
Chinese medicine composition provided by the invention is using three Chinese medicine preparations treatment grouper vibrio alginolyticus disease simultaneously, with folk prescription
The advantages that Chinese medicine therapeutic effect compares, and has treatment time shorter, and curative effect is better, fewer than pure western medicine medicament residue.
The present invention provides the preparation methods of the Chinese medicine composition, and traditional Chinese medicinal components myrobalan, Radix Paeoniae Alba and Radix Glycyrrhizae are successively passed through
Crushing is crossed, pulverizes and toasts, Chinese medicine composition is prepared in obtained traditional Chinese medicine fine powder mixing.The preparation method is advantageous
It is sufficiently discharged in traditional Chinese medicinal components pharmaceutical active ingredient, efficacy of drugs is improved, while the method preparation step is simple, using machine
Tool processing, it is reproducible, it is advantageously implemented mass production.
The present invention provides application of the Chinese medicine composition in the drug of preparation treatment grouper vibrio alginolyticus disease.Institute
The growth of vibrio alginolyticus can effectively be inhibited by stating Chinese medicine composition, to realize that the drug of preparation effectively treats grouper vibrio alginolyticus
The purpose of disease.
Specific embodiment
The present invention provides the Chinese medicine composition for the treatment of grouper vibrio alginolyticus disease, including myrobalan, Radix Paeoniae Alba and Radix Glycyrrhizae；It is described to scold
Son, Radix Paeoniae Alba and the mass ratio of Radix Glycyrrhizae are 0.9~1.3：0.9~1.3：0.9~1.3, preferably 1：1：1.
The present invention is not particularly limited the source of the myrobalan, Radix Paeoniae Alba, Radix Glycyrrhizae, and use is well known to those skilled in the art
Myrobalan, Radix Paeoniae Alba, Radix Glycyrrhizae.In embodiments of the present invention, the myrobalan, Radix Paeoniae Alba and Radix Glycyrrhizae are purchased from Xiamen Lian Futang pharmacy.
The present invention provides the preparation methods of the Chinese medicine composition, include the following steps：
1) myrobalan, Radix Paeoniae Alba and Radix Glycyrrhizae are crushed, obtains herb powder；
2) herb powder is pulverized, obtains ultramicro medicine powder；
3) ultramicro medicine powder is toasted to 24~48h under the conditions of 60~70 DEG C, obtained traditional Chinese medicine fine powder is Chinese traditional medicine composition
Object.
The present invention crushes myrobalan, Radix Paeoniae Alba and Radix Glycyrrhizae, obtains herb powder.The partial size of smashed herb powder is preferably
140~160 μm.More preferably 150 μm.It include that myrobalan, Radix Paeoniae Alba and Radix Glycyrrhizae crush respectively or by myrobalan, Radix Paeoniae Alba when the crushing
It is crushed again with Radix Glycyrrhizae according to said ratio mixing.The present invention is not particularly limited the method for the crushing, using this field skill
Breaking method known to art personnel.In embodiments of the present invention, the crushing that the crushing is model FW177 with instrument
Machine is purchased from Tianjin Stettlen Instrument Ltd..
After obtaining herb powder, the present invention pulverizes the herb powder, obtains ultramicro medicine powder.
In the present invention, preferably the herb powder is toasted before the ultramicro grinding.The temperature of the baking is excellent
It is selected as 60~70 DEG C, more preferably 65 DEG C.The time of the baking is preferably 24~48h, more preferably 36h.Baking makes ultra micro
The water content of medicinal powder reduces, and makes the requirement for meeting ultramicro grinding.
In the present invention, the partial size of the ultramicro medicine powder is preferably 5~10 μm, more preferably 6~9 μm, most preferably 8 μ
m.The present invention is not particularly limited the method for the ultramicro grinding, and the method using ultramicro grinding known in the art is
It can.In embodiments of the present invention, the ultramicro grinding vibration pulverizing mill clearly of three that instrument is model SQW-60, is purchased from Shandong
Three clear stainless steel equipment Co., Ltds.The ultramicro grinding is conducive to active ingredient of Chinese herbs and sufficiently releases.
After obtaining ultramicro medicine powder, the ultramicro medicine powder is toasted 24~48h by the present invention under the conditions of 60~70 DEG C, is obtained
Drug composition.In the present invention, it is 65 DEG C that the temperature of the baking is excellent, and the excellent time of the baking is 36h.Baking makes ultra micro medicine
The water content of powder reduces.
The present invention provides application of the Chinese medicine composition in the drug of preparation treatment grouper vibrio alginolyticus disease.
In the present invention, the Chinese medicine composition includes the following steps the bacteriostatic experiment of grouper vibrio alginolyticus：It is described
Chinese medicine composition is formulated as solution, by obtained Chinese medicine composition solution to 0.1mL bacterium amount concentration 108The vibrio alginolyticus of CFU/mL
It instills plate, smoothen, observe antibacterial situation.As a result, it has been found that it is pole that the effective concentration of Chinese medicine composition solution, which is 200~240mg/L,
It is sensitive.The effective concentration of Chinese medicine composition solution is more preferably 210~230mg/L, most preferably 220mg/L.
In the present invention, the preparation method of the drug preferably includes following steps：Feed fine powder is made in feed, it will be upper
The Chinese medicine composition for stating scheme preparation is mixed with feed fine powder, is pelletized, is obtained drug.The present invention does not have the mixed method
It is specifically limited, using mixed method known in the art.The present invention is not particularly limited the method for the granulation, adopts
With method of granulating known in the art.
In the present invention, the quality of Chinese medicine composition preferably accounts for the 1.8%~2.4% of drug gross mass, more preferably
2.0%.The feeding amount of described pharmaceutical composition is preferably the 2%~3% of quality of fishes, and more preferably 2.5%.The Chinese medicine group
Conjunction object, which is added in Epinephelus coioides basal feed, is made into Chinese medicine particulate material.
In the present invention, the administration time of the drug therapy grouper vibrio alginolyticus disease is preferably 30~45d.
Below with reference to embodiment to provided by the invention a kind of for treating the composition of grouper vibrio alginolyticus disease
And its preparation method and application be described in detail, but they cannot be interpreted as limiting the scope of the present invention.
Embodiment 1
Myrobalan, Radix Paeoniae Alba, Radix Glycyrrhizae are bought from Xiamen Lian Futang pharmacy；Medicinal medicinal herb grinder crushes (FW177, Tianjin by
Stettlen Instrument Ltd. of city) be crushed to about 150 μm, be then placed in micronizer (three clear vibration pulverizing mills, SQW-60,
Sanqing Stainless Steel Apparatus Co., Ltd., Shandong) in further crush, until grain diameter size be 5~10 μm, collect ultra micro medicine
Powder is spare.Three kinds of ultramicro medicine powders are toasted into 48h under the conditions of 60 DEG C respectively, obtain traditional Chinese medicine fine powder；It weighs obtained above
Traditional Chinese medicine fine powder, myrobalan fine powder 0.9kg, Radix Paeoniae Alba fine powder 1.3kg, Radix Glycyrrhizae fine powder 0.9kg obtain Chinese medicine composition.
Embodiment 2
The Chinese medicine of myrobalan, Radix Paeoniae Alba, Radix Glycyrrhizae is crushed to 160 μm, obtains three kinds of herb powders；By three kinds of herb powders
It is pulverized respectively to 10 μm of partial size, obtains three kinds of ultramicro medicine powders；By three kinds of ultramicro medicine powders respectively in 70 DEG C of conditions
Lower baking for 24 hours, obtains traditional Chinese medicine fine powder；Weigh traditional Chinese medicine fine powder obtained above, myrobalan fine powder 1.2kg, Radix Paeoniae Alba fine powder 0.9kg are sweet
Careless fine powder 1.0kg, mixing, obtains Chinese medicine composition.
Embodiment 3
The Chinese medicine of myrobalan, Radix Paeoniae Alba, Radix Glycyrrhizae is crushed to 150 μm, obtains three kinds of herb powders；By three kinds of herb powders
It is pulverized respectively to 8 μm of partial size, obtains three kinds of ultramicro medicine powders；By three kinds of ultramicro medicine powders respectively under the conditions of 65 DEG C
36h is toasted, traditional Chinese medicine fine powder is obtained；Weigh traditional Chinese medicine fine powder obtained above, myrobalan fine powder 1.0kg, Radix Paeoniae Alba fine powder 1.0kg, Radix Glycyrrhizae
Fine powder 1.0kg mixing, obtains Chinese medicine composition.
Comparative example 1
Myrobalan traditional Chinese medicine fine powder 1.0kg prepared by embodiment 3 is weighed, folk prescription drug is obtained.
Comparative example 2
Radix Glycyrrhizae fine powder 2.0kg and Radix Paeoniae Alba fine powder 2.0kg prepared by embodiment 3 are weighed, compound bigeminy Chinese medicine composition is obtained.
Comparative example 3
Fructus Forsythiae is bought from Tianjiang Pharmaceutical Industry Co., Ltd., Jiangyin, Radix Astragali traditional Chinese medicine fine powder is prepared according to the method for embodiment 3,
Radix Astragali traditional Chinese medicine fine powder 1.5kg is weighed, the Radix Paeoniae Alba fine powder 1.5kg and Radix Glycyrrhizae fine powder 1.5kg prepared with embodiment 3 is mixed, and is answered
Fang Sanlian Chinese medicine composition.
Comparative example 4
With Odontothrips loti, Enrofloxacin medicine in the K-B agar diffusion method of the paper (osmanthus Ping Dong etc., 2001) recommended referring to WHO
Concentrations above drug is added in the drug concentration of the quick scraps of paper in every hole.Enrofloxacin (the limited public affairs of Xinghua City perseverance prestige biotechnology
Department).By 1 × 108200 μ L bacteria suspension of CFU/mL vibrio alginolyticus is spread evenly across on LB culture medium flat plate, according to CLSI M100's
Newest standards determine result.Antibacterial circle diameter is measured, determines the sensibility of vibrio alginolyticus.
Embodiment 4~6
Composition prepared by embodiment 1 is mixed with water, and water proof heat, be prepared into solution be equivalent to crude drug quality it is dense
Degree is 200mg/ml, 220mg/ml and 240mg/ml.Composition prepared by embodiment 2 and 3 is equally prepared into according to the method described above
The drug solution of 200mg/ml, 220mg/ml and 240mg/ml.
Using Odontothrips loti research drug to the function and effect of bacterium, the LB after 10~12mL sterilizing is poured into plate is solid
Body culture medium after to be solidified, be gently put into 4 Oxford cups in each culture dish moderate distance with tweezers and immerse in culture medium, take
The LB culture medium (temperature is advisable with non-scald on hand) of 12~15mL is uniformly mixed and pours on plate, and after to be solidified, Oxford cup is removed,
Vibrio alginolyticus is diluted to 1.0x108~3.0x108CFU/mL draws 0.1mL bacteria suspension with liquid-transfering gun, plate is instilled, with coating
Device smoothens, and it is control that the sterile water of 200 μ L is added in 1 hole with liquid-transfering gun, is added above-mentioned 3 in another 3 Kong Zhongyong liquid-transfering guns
The 200 μ L of medical fluid of kind various concentration, each test group and control group are respectively provided with 3 repeating groups.It is placed under the conditions of 26~30 DEG C, it is permanent
Temperature 18~36h of culture, observes inhibition zone, and with SupcreG1 bacterium colony counting/screening/inhibition zone measurement combined instrument (fast number in Hangzhou
Science and Technology Ltd.) measurement inhibition zone size, 3 repetitions of each experiment.Average value is calculated, research is compared, according to antibacterial
Loop diameter size determines the power of its bacteriostasis.Inhibition zone the results are shown in Table 1.
The antibacterial situation of the composition various concentration solution of 1 Examples 1 to 3 of table preparation
Comparative example 5~8
Bacteriostatic experiment is carried out according to the Chinese Medicines that the method for embodiment 4~6 prepares comparative example 1~4, observes inhibition zone
And bacteriostasis.It the results are shown in Table 2.
The antibacterial situation of the composition various concentration solution of 2 comparative example 1~4 of table preparation
Embodiment 7~11
By the Chinese medicine composition of Examples 1 to 3 preparation respectively with grouper basal feed according to 1.8~2.4：98.2~
97.6 mass ratio mixing, granulation obtain 3 groups of drugs.
Every tail tests grouper with 103The vibrio alginolyticus viable bacteria bacteria suspension of CFU/g is by way of intrathecal to tested
Fish carries out attacking poison.Vibrio alginolyticus living bacterial liquid 0.2mL is attacked after poison and is fed basal feed for every group, feeds 40d, obtain with molten algae
The grouper of vibrio infection.Each aquaculture pond launches the grouper that 20 tails suffer from vibrio alginolyticus infection.
Test group 1~3 feeds 3 groups of drugs of above-mentioned preparation, and specific feeding relationship is shown in Table 3, and scale of feeding is equivalent to Chinese medicine group
The feeding volume for closing object accounts for 2%~3% of grouper weight in aquaculture pond, and the number fed daily is 2 times, feeds 40d altogether.To fish
Residual bait is sucked out on a full stomach.Day changes that water is primary, and quantity of exchanged water is the 1/3 of total volume.Control group I is also set up simultaneously (to raise on normal basis of feeding
Material, healthy fish) and control group II (normally feeding basal feed, vibrio alginolyticus attacks malicious fish).
3 test group 1~3 of table feeds the cumulative mortality of 3 groups of drugs and control group of above-mentioned preparation
Embodiment 12
Manually attack poison and Chinese herb prevention grouper vibrio alginolyticus disease：
1, artificial liver support:Before experiment starts, experiment Epinephelus coioides injection vibrio alginolyticus is carried out attacking malicious preliminary experiment,
80% or more occurs illness but dead concentration is not caused to be set to experiment bacterial concentration.Epinephelus coioides are purchased from Zhangpu city of Fujian Province
Certain farm, long (11 ± 2) cm of body, temporarily supports in the circulating water culture system of 1t water body.For testing after temporarily supporting 1 week.Weight,
The comparable healthy Epinephelus coioides of Individual Size are randomly divided into experimental group and control group, every group of each 20 tail, follow respectively at 18 DEG C
Adaptive feeding one week in ring water cultivating system.With 103The vibrio alginolyticus bacteria suspension of CFU/g by way of intrathecal to by
Examination fish carries out attacking poison.Control group Epinephelus coioides are then injected with PBS buffer solution.Then 4 groups of fishes are transferred in raising water body respectively
It is fed.Test sets control group I (feeding material, healthy fish), control group II (feeding material, vibrio alginolyticus attack malicious fish), test group I
(feeding material plus the Chinese medicine of the preparation of embodiment 1 are combined compound, and vibrio alginolyticus attacks malicious fish), is separately treated with common drug in production
Contrast on effect, if control group III (feeding is expected plus 2.2% plate Huang dissipates, and vibrio alginolyticus attacks malicious fish), control group IV (are fed with material to add
2.2% rheum officinale Chinese gall dissipates, and vibrio alginolyticus attacks malicious fish), each test group and control group are respectively provided with 3 repeating groups.On-test
Afterwards, daily in 8:00,17:00 feeds 2 times, feeding amount be quality of fishes 2%~3%, and according to grow, situation of ingesting is adjusted
It is whole.Residual bait is sucked out on a full stomach to fish.Day changes that water is primary, and quantity of exchanged water is the 1/3 of total volume.Experimental basis feed is provided by company.
Test feed respectively in add Chinese herbal formula control group feed in do not add.
It keeps observing and recording survival condition (observation 40d) daily, records disease symptom and death condition, dissection checks dead
Fish is died, is calculated cumulative mortality (Cumulative mortalityrate).
RCM=[(N0-Nt)/N0] × 100% formula a
In formula a:RCM is cumulative mortality (%)；N0 is to test just fish number；Nt is off-test fish number；Correlation is carried out to refer to
Mark detection.But fish continues to feed with upper type if there is survival, until fish is all dead.The survey death rate (initial fish survival rate,
Half fish death time, whole fish death times).
2, the result of pharmaceutical chemistry group and the survival rate of control group fish
Control group and the test group after viable bacteria artificial challenge's Epinephelus coioides, the death condition of each group are as shown in table 4.
For challenge test result after challenge test 6 days, there is death successively in Epinephelus coioides experimental group, and test fish starts
It now falls ill phenomenon.
Test proves that the death for attacking fish in 40d after poison tends towards stability, therefore the dead of each group in 40d is attacked after poison in this test data sheet
Die number, attack poison the 1st day and the 2nd day each test group fish food ration significantly reduce, sick fish The dead quantity control group II>Control group IV
>Control group III>Test group I>Control group I.
When off-test, control group I (blank control group, health fish feed material) health is survived, and survival rate is
100.00%, and activity and normal, the illness being visible by naked eyes of ingesting.
There is dead 1 tail in the 6d after bacteria suspension injection of control group II, and 7~25d is peak mortality phase, dead 20 tail.
There is dead 1 tail in the 8d after bacteria suspension injection of test group I, and 26d is the peak mortality phase.44d dead 100%.And it adopts
It is respectively 85.00%, 95% with common medicine treatment group control group III, the IV 40d death rate of control group, and its death rate is higher than this hair
The Chinese medicine composition medication of bright offer.
The Epinephelus coioides resistance that Chinese herbal medicine is added in feed is apparently higher than control group, and therapeutic effect is common better than production
Medicine plate Huang dissipates, rheum officinale Chinese gall dissipates.
The survival rate result of table 4 pharmaceutical chemistry group and control group Epinephelus coioides
The experimental results showed that：Feeding material adds myrobalan, Radix Paeoniae Alba, traditional Chinese medicine of licorice root compound that can preferably treat the molten algae arc of Epinephelus coioides
Bacterium disease.Better than producing, common medicine plate Huang dissipates therapeutic effect, rheum officinale Chinese gall dissipates.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (9)
1. a kind of Chinese medicine composition for treating grouper vibrio alginolyticus disease, which is characterized in that including myrobalan, Radix Paeoniae Alba and Radix Glycyrrhizae；Institute
The mass ratio for stating myrobalan, Radix Paeoniae Alba and Radix Glycyrrhizae is 0.9~1.3：0.9~1.3：0.9~1.3.
2. Chinese medicine composition according to claim 1, which is characterized in that the mass ratio of the myrobalan, Radix Paeoniae Alba and Radix Glycyrrhizae is
1：1：1.
3. the preparation method of Chinese medicine composition as claimed in claim 1 or 2, which is characterized in that include the following steps：
1) myrobalan, Radix Paeoniae Alba and Radix Glycyrrhizae are crushed, obtains herb powder；
2) herb powder is pulverized, obtains ultramicro medicine powder；
3) ultramicro medicine powder is toasted to 24~48h under the conditions of 60~70 DEG C, obtains Chinese medicine composition.
4. preparation method according to claim 3, which is characterized in that the partial size of herb powder is 140~160 μ in step 1)
m；
The partial size of ultramicro medicine powder is 5~10 μm in the step 2).
5. according to the preparation method of claim 3 or 4, which is characterized in that the temperature toasted in the step 3) is 65 DEG C, institute
The time for stating baking is 36h.
6. application of the Chinese medicine composition as claimed in claim 1 or 2 in the drug of preparation treatment grouper vibrio alginolyticus disease.
7. application according to claim 6, which is characterized in that effective Mlc of the Chinese medicine composition be 200~
240mg/L。
8. application according to claim 6, which is characterized in that the quality of the Chinese medicine composition accounts for drug gross mass
1.8%~2.4%.
9. application according to any one of claims 6 to 8, which is characterized in that the molten algae arc of drug therapy grouper
The administration time of bacterium disease is 30~45d.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810901292.4A CN108815241A (en) | 2018-08-09 | 2018-08-09 | It is a kind of for treating the Chinese medicine composition and its preparation method and application of grouper vibrio alginolyticus disease |
Applications Claiming Priority (1)
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| CN112656854A (en) * | 2021-02-08 | 2021-04-16 | 集美大学 | Traditional Chinese medicine composition for preventing and treating fish visceral ichthyophthiriasis and preparation method and application thereof |
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Application publication date: 20181116 |