CN1284372A - 'Sanhuangqinbao' powder-medicine for chicken - Google Patents
'Sanhuangqinbao' powder-medicine for chicken Download PDFInfo
- Publication number
- CN1284372A CN1284372A CN 99112359 CN99112359A CN1284372A CN 1284372 A CN1284372 A CN 1284372A CN 99112359 CN99112359 CN 99112359 CN 99112359 A CN99112359 A CN 99112359A CN 1284372 A CN1284372 A CN 1284372A
- Authority
- CN
- China
- Prior art keywords
- medicine
- chicken
- powder
- radix
- group
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Fodder In General (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The medicine powder of the present invention is used to treat various chicken diseases. It is prepared by using 12 kinds of Chinese medicinal materials including Radix scutellariae, Rhizoma Coptidis, Cortex Phellodendri, Cape jasmine, Chinese angelica, etc. and through drying, grinding, mixing and disinfection. It has unique formulation and preparation process and obvious curative effect.
Description
The present invention relates to a kind of medicine for the treatment of multiple fowl disease, specifically a kind of is the Chinese patent medicine of feedstock production with the Chinese herbal medicine.
Development along with developing country's scale poultry husbandry; chicken group's sickness rate increases year by year; for example: bacterial diseasees such as the colibacillus disease of poultry, salmonellosis, infectious bursal disease, the white scour of chicken, green pus liver bacterium, fowl cholera, pair typhoid fever, staphylococcosis, streptococcicosis; viral infection such as bronchitis; the mould forage poisoning etc. that loses makes fowl diseases more complicated.Comprehensive infection takes place in multiple diseases such as some bacterial disease, viral sexually transmitted disease (STD) and mycete sexually transmitted disease (STD) often clinically.Prior art treat above-mentioned various fowl disease adopt to unite use antibiotics to treat, still, therapeutic effect is unsatisfactory, also causes Drug resistance and other side effect easily.At present, the middle one-tenth veterinary drug preparation that circulates on the market adds western medicine composition mostly, and medicine residual hazard problem is left in the same existence in clothes back, and the meat egg quality is descended, and is detrimental to health, and influences foreign export.
In addition, a large amount of uses of antibiotics class medicine, Abwehrkraft des Koepers, fertility performance and product quality are descended, chicken raising cost improves, economic benefit reduces, and some bacterial disease and viral sexually transmitted disease (STD) or mycosis etc. usually are cross infections, and list is treated with antibiotics clinically, sometimes be difficult to prove effective, thereby have a strong impact on the development of aquaculture.
The purpose of this invention is to provide a kind of treatment colibacillus disease, salmonellosis, infectious bursal disease, the white scour of chicken, green pus liver bacterium, fowl cholera, pay viral infection fowl disease and the mould precious Chinese medicine preparation that loose of yellow fowl that lose fowl disease such as forage poisoning such as bacterial diseasees such as typhoid fever, staphylococcosis, streptococcicosis, bronchitis.
The objective of the invention is to realize in the following manner, according to the modern scientific research data, some kind of China's Chinese medicine has inhibitory action to various antibacterials, virus, pathogenic fungus, tumor etc.Through scientific composition and rational use of drug, can execute effectively preventing to the fowl diseases row equally, reduce medication, reduce production costs.Make great efforts to excavate motherland's Chinese medicine treasure-house, advocate energetically and develop the fully natural green medicine, fundamentally can solve the poison of drug residue problem, improve meat egg product matter, for the mankind benefit.
In poultry disease prevention and control work, except that adhering to carrying out all routine immunizations, also should pay attention to improving Abwehrkraft des Koepers, really " putting prevention first and combining prevention with control " are fulfilled, improve the aquaculture economic benefit.
China is the country that abounds with Chinese medicine, and the history in thousands of years is arranged, aboundresources, raw material abundance.The Chinese medicine of respectively distinguishing the flavor of in the prescription all belongs to pure natural substance, and stable in properties, manufacturing process do not add any chemical substance, and pulverizing does not change its original character after mixing.
Multi-flavor medicine in the prescription has antibacterial mildew-resistant effect, and therefore, its preparation nature is stable, under normal circumstances a year and a half shelf-life.
The property of medicine and pharmacology analysis:
Radix Scutellariae:
Composition: contain baicaligenin, baicalin, baicalein, wogonoside, wogonin, neobaicalein etc.
1, have analgesic, calm, diuresis, function of gallbladder promoting and reduction capillary permeability, alleviate effects such as enterospasm.
2, the in-vitro antibacterial test all has inhibitory action to dysentery bacterium, diphtheria corynebacterium, bacillus pyocyaneus, staphylococcus, Hemolytic streptococcus, Diplococcus pneumoniae, influenza virus and skin pathogenicity fungus etc.
3, baicaligenin, baicalin all have mitigation to contraction of guinea-pig isolated trachea anaphylaxis and whole animal allergic asthma, and collaborative with the ephedrine performance; Cavia porcellus passivity skin allergy, histamine's dermoreaction are also showed inhibitory action.
4, the baicalin subcutaneous administration can reduce the toxicity of strychnine to mice, significantly improves the hypodermic median lethal dose(LD 50) of strychnine.
5, the oral decoct 10 gram/kilograms of rabbit, quiet notes ethanol extract 2 gram/kilograms, none example is dead except that behaving with composure.
Rhizoma Coptidis:
Composition: contain alkaloids such as berberine 7-9%, coptisine, 13-methyl-.psi.-coptisine., palm leaf coculine, paAlmatine.
1, Hemolytic streptococcus, meningococcus, Diplococcus pneumoniae, staphylococcus aureus all there is strong inhibitory action; Dysentery bacterium, diphtheria corynebacterium, bacillus subtilis, viridans streptococci etc. all there is inhibitory action; Streptococcus pneumoniae, bacillus pestis, Brucella, clostridium tetani, bacillus perfringens, trypanosomicide etc. also there is inhibitory action.
2, can strengthen the phagocytic activity of leukocyte and reticuloendothelial system.
3, berberine has analgesic, diuresis, local anesthesia, calmness, analgesia, antitumor and choleretic effect.
4, the LD50 of mouse peritoneal injection is 24.3 milligrams/kilogram.
Cortex Phellodendri:
Composition: contain the about 1.6-4% of berberine, jateorhizine, magnoflorine, phellodendrine etc.
1, antimicrobial spectrum is similar with Rhizoma Coptidis with antimicrbial power.Also effective to pathogenic dermatophytes, leptospira, trichomonas vaginitis.
2, platelet is had protective effect, it is broken that it is difficult for.
3, external can promote the absorption of subcutaneous oozing of blood.
4, function of gallbladder promoting, diuresis, blood vessel dilating and antipyretic effect are arranged.
5, the LD50 of mouse peritoneal injection is 2.7 gram/kilograms.
Fructus Gardeniae:
Composition: contain flavonoid gardenin, fruit acid, crocin, crocetin, gardenoside, Fructus Gardeniae time glycoside etc.
1, choleretic effect is arranged, can increase the gallbladder hidropoiesis, behind the ligation ductus choledochus, blood mesobilirubin is reduced, its effective ingredient is crocin and crocetin.
2, experiment in vitro has inhibitory action to dysentery bacterium, staphylococcus aureus, bacillus pyocyaneus and some dermatophytes.
3, anastalsis is arranged, and Fructus Gardeniae is stronger than Fructus Gardeniae Preparatus effect, external can be quickened the healing of soft tissue.
4, the acute LD 50 of mouse subcutaneous injection Fructus Gardeniae fluid extract is 31.79 gram/kilograms.The acute toxicity of Fructus Gardeniae aqueous extract is less, and lumbar injection 5 gram/kilograms all do not have animal dead.
Composition: contain the hydrolysis tannin, comprise different gallic acid, gallic acid, yellow acid, chebulinic acid.
In vitro tests has strong inhibitory action to dysentery bacterium etc.
Radix Et Rhizoma Rhei:
Composition; Mainly contain anthraquinone derivative, its content is 3-5%, and chrysophanic acid, chrysophanol, emodin, aloe-emodin, physcione etc. are arranged.Tannin has catechotannin, gallic acid Cortex cinnamomi japonici (Ramulus Cinnamomi) tannin, anthraquinone tannin etc.
1, the experiment of rat and Cavia porcellus myenteron shows that free Radix Et Rhizoma Rhei have the effect of similar phatidylcholine sample.Radix Et Rhizoma Rhei cathartic effective ingredient is an anthraquinone derivatives, and wherein the Senna fruit glycoside is the strongest effective ingredient of discharge function.It is 117 milligrams/kilogram that extractum rhei is irritated stomach half diarrhea inducing amount ED50.
2, low dose has the gastric secretion of promotion and astringing to arrest diarrhea effect.
3, spasmolytic diuresis, function of gallbladder promoting and antitumor action are arranged.
4, all inhibited to 13 kinds of antibacterials such as staphylococcus \ Hemolytic streptococcus \ dysentery bacterium etc. and conventional pathogenic bacterium and stream virus etc.
5, the astringing to arrest bleeding effect is arranged.
Composition: it is about 1.5% that the Rhizoma Atractylodis Macrocephalae contains volatile oil, is mainly atractylol, atractylone, atractylodes lactone, first and second, retinoid material.
1. the feedstuff of forming with the Rhizoma Atractylodis Macrocephalae adds agent, feeds chicken after 28 days, and the meat feed ratio of matched group is 1: 3.60; The meat material of test group 1: 2.72;
2. mouse stomach Rhizoma Atractylodis Macrocephalae decoct January, can promote weight increase, swimming endurance strengthens;
3. excite and promote immunization: improve lymhocyte transformation rate and natural Flos Rosae Rugosae formation rate, serum 1gG obviously increases;
4. mice decoct for oral administration has the liver protecting, prevents the liver glycogen minimizing effect that carbon tetrachloride causes; 5. the neutral oil of Rhizoma Atractylodis Macrocephalae volatile oil has obvious inhibitory action to esophageal cancer cell;
6. the drainage of remarkable and persistent diuresis, particularly sodium is arranged;
7. the blood vessel dilating effect is arranged, heart is inhibitory action;
8. the acute SD50 of Rhizoma Atractylodis Macrocephalae decoct mouse peritoneal injection is 73.3 gram/kilograms.
Radix Angelicae Sinensis:
Composition: contain ligustilide, n-butyl alcohol Xi Fu lactone, positive valeryl benzene phosphorus carboxylic acid, n-dodecane alcohol, bergamot lactone, fatty oil, Palmic acid, cupreol, cetylate, sucrose, vitamin B
12Vitamin A, E and Yan acid, folic acid, folinic acid, biotin, ferulic acid, succinic acid, uracil, adenine.
1, use flame atomic absorption spectrometry, the content of Radix Angelicae Sinensis (Zhejiang product) ferrum is 400ppm, and the content of copper is 6.0ppm, and the content of Dou is 17.5ppm.
2, contained vitamin B
12And Yan acid, folic acid, folinic acid have anti-anemia effect.
3, the uterus had " amphicheirality " effect, be suppressed to and be divided into high boiling point (180-210 ℃) volatile oil, the uterus rhythmicity is shunk to be reduced, uterus muscle is relaxed, excited composition is water or pure dissolubility nonvolatile matter, uterine contraction is strengthened, during a large amount of or multiple dosing, the contraction of tetanic property can be occurred.
4, the contained volatile oil of decoct has inhibitory action to the Bufo siccus frog heart that exsomatizes.
5, eumenol can make coronary artery expansion to the dirty perfusion of guinea-pig heart, and blood flow increases; Reduce platelet aggregation and thrombosis.
6, can significantly strengthen the hypertrophy of initial immunity mouse rosette-forming cells.
7, the effect of vitamin antagonist E shortage is arranged.
8, the Radix Angelicae Sinensis decoct has inhibitory action to escherichia coli, dysentery bacterium, typhoid fever and Salmonella paratyphi.Its volatile oil has inhibitory action to infecting mouse preventive administration or the treatments of infection back such as staphylococcus aureus, escherichia coli, bacillus pyocyaneus.
' Sanhuangqinbao ' powder-medicine for chicken of the present invention is made by following component: (consumption attach most importance to part)
Radix Scutellariae 10-20 Rhizoma Coptidis 10-20 Cortex Phellodendri 4-10
Fructus Gardeniae 10-20 Radix Angelicae Sinensis 4-10 Rhizoma Atractylodis Macrocephalae 1-6
Radix Et Rhizoma Rhei 4-10 Fructus Chebulae 4-10 Radix Paeoniae Alba 4-10
Cortex Cinnamomi 1-6 Poria 4-6 Rhizoma Chuanxiong 2-6
Formula optimization weight (part) ratio range of preparation medicine of the present invention is:
Radix Scutellariae 15-20 Rhizoma Coptidis 10-18 Cortex Phellodendri 4-8
Fructus Gardeniae 14-20 Radix Angelicae Sinensis 6-10 Rhizoma Atractylodis Macrocephalae 3-6
Radix Et Rhizoma Rhei 4-8 Fructus Chebulae 6-10 Radix Paeoniae Alba 6-10
Cortex Cinnamomi 3-6 Poria 2-4 Rhizoma Chuanxiong 4-6
Weight limit (part) proportioning of medicine of the present invention is:
Radix Scutellariae 18 Rhizoma Coptidis 16 Cortex Phellodendris 5
Fructus Gardeniae 16 Radix Angelicae Sinensis 8 Rhizoma Atractylodis Macrocephalaes 7
Radix Et Rhizoma Rhei 5 Fructus Chebulae 6 Radix Paeoniae Albas 8
Cortex Cinnamomi 4 Poria 3 Rhizoma Chuanxiongs 4
Above-mentioned component made medicine production method of the present invention is: by the prescription requirement, every medicine is ground into 120 purpose fine powders, stirs, sterilized 10-20 minute, make powder.
Clinical drug of the present invention uses the result to show that following advantage is arranged:
1, to select natural plants for use be raw material in the present invention, and each component meets the pharmaceutical control law regulation, utilizes the comprehensive function of the Chinese medicine of respectively distinguishing the flavor of to treat various fowl disease, nontoxic to the fowl poultry.
2, need not decoct during drug use of the present invention, taking convenience meets the pertinent regulations of national veterinary drug management.
For showing the therapeutic effect of medicine of the present invention to various fowl disease, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricul and institute of the Shandong Province's veterinary drug supervision institute clinical experiment report of doing are as follows;
The extracorporeal bacteria inhibitor test clinical experiment report:
One, test objective:
Chinese medicine preparation of the present invention is a pure Chinese medicinal preparation, and multiple fowl bacterial disease and virosis are had prevention and therapeutic effect.This test is intended to verify the prevention effect of this medicine to commonly encountered diseases, external fungistatic effect, and, the using dosage and the course of treatment are proposed to the therapeutic effect of chicken colibacillosis, salmonellosis.
Two, test material:
1, trial drug: ' Sanhuangqinbao ' powder-medicine for chicken, specification 500g/ bag, lot number: 980901, association provides by the Shandong poultry.
2, control drug: QINJUNLING, specification: the 250g/ bag, lot number: 980126, Laiwu City animal pharmaceutical factory in Shandong produces: Shandong poultry association provides.
3, test chicken: sea blue white laying hen 10 ages in days are differentiated young ♂, are used for artificial onset's treatment, are provided by the herding of academy of agricultural sciences, Shandong Province.5 age in days Hai Lanbai laying hens are used for prophylactic tria, carry out in academy of agricultural sciences, Shandong Province herding institute test site.
4, seed culture of viruses: escherichia coli O
1, O
2, O
78Salmonella; Haemophilus paragallinarum, this institute preserves.
5, culture medium: ordinary broth, nutrient agar panel, chocolate agar plating medium.
Three, extracorporeal bacteria inhibitor test: (, carrying out bacteriostatic test) with the method for digging a hole by 2 dosage forms
1, medicine preparation:
(1) get ' Sanhuangqinbao ' powder-medicine for chicken 5g, 2 penicillin bottles of packing into through 8 pounds of sterilizations in 15 minutes, are put in the refrigerator standby.
(2) get the precious 10g of loosing of fowl and put in the 100ml beaker, add water to 100ml, soaking at room temperature 30min, slow fire boils 1h, filters, and adds commensurability water in the medicinal residues again, boils 1h again, filters, and twice medicinal liquid mixed, and evaporation and concentration is to every milliliter of stock solution that is equivalent to 1g crude drug concentration.Through 8 pounds of 15min sterilizations, it is standby to put refrigerator.
(3) get the control drug QINJUNLING with quadrat method and produce medicinal liquid and powder.
2, test method:
(1) dips in the escherichia coli of getting cultivation 18h with oese and evenly coat the ordinary nutrient agar plating medium; Dip in get cultivate 18h the haemophilus paragallinarum separate application in the chocolate agar plating medium.
(2) metal tube with diameter 10mm burrows on culture medium, and adds the sterilization agar back cover of a little fusing at the bottom of the hole.Add the medicine that desire is surveyed in the hole, decoct is the 0.1ml/ hole, and powder is filled it up with the hole, puts to cultivate observed result behind the 24-28h in 37 ℃ of incubators.
(3) antibacterial criterion as a result: average antibacterial circle diameter＜12mm is a hypoallergenic, and 15mm is quick in being, 15-20mm is for extremely quick.
Four, clinical trial method:
1, artificial onset: get three strain meat soups and cultivate the escherichia coli mixed liquor of 18h and the Salmonella liquid of isodose, be made into 100,000,000/ml bacterium drinking-water counteracting toxic substances, average every chicken 2ml with tap water.
2, get 300 of test chickens, observed 3 days, be divided into 6 groups at random, 50 every group, each organizes test method and dispensing dosage is as follows:
(1): high concentration treatment group, 24h behind the artificial challenge, feeding contains precious diffusing 2.5% the feedstuff of fowl.
(2): middle concentration treatment group, 24h behind the artificial challenge, feeding contains precious diffusing 2% the feedstuff of fowl.
(3): low concentration treatment group, 24h behind the artificial challenge, feeding contains precious diffusing 1.5% the feedstuff of fowl.
(4): the medicine matched group, 24h behind the artificial challenge, feeding contains the feedstuff of QINJUNLING 0.6%.
(5): not administration of artificial challenge group, the conventional feed of feeding.
(6): the blank group, do not infect not medication, the conventional feed of feeding.Each dispensing group free choice feeding serve on 5 days.
3, commonly encountered diseases prophylactic tria, testing a building in this institute broods indoor, select 345 of the sea blue brown laying hens of 5 ages in days, feeding contains the precious complete feed 5 days of loosing of 1% fowl, drug withdrawal 10 days, 5 days one month experimental periods of repeat administration, do not re-use other drug, the contrast chicken is fed and is not contained the precious complete feed that looses of fowl, prevents bacterial diseases such as Salmonella with gentamycin at 7 ages in days, 28 ages in days prevent coccidiosis with the spirit of ball dysentery, and two groups impose identical immune programme for children and feeding and management measure.
4, clinical observation: artificial onset's treatment group was observed 10 days continuously, the incidence of each group of record, and clinical manifestation, death toll, and cut open inspection and observe pathological changes, determine the cause of the death.
Prophylactic tria is observed two groups the speed of growth, incidence, statistical weight and survival rate.
5, curative effect index: the statistics artificial onset cure rate, effective percentage, protective rate, each group is carried out X
2Check.
Four, result
1, extracorporeal bacteria inhibitor test: on the nutrient agar panel of coating chicken colibacillosis, the decoct of ' Sanhuangqinbao ' powder-medicine for chicken and QINJUNLING and powder inhibition zone are respectively 12cm, 13cm and 11cm, 12cm.On the chocolate agar flat board of coating haemophilus paragallinarum, tangible inhibition zone appears in the decoct of QINJUNLING and powder, and the inhibition zone of ' Sanhuangqinbao ' powder-medicine for chicken powder, decoct is respectively 15cm, 14cm.
2, test chicken 30-40h behind artificial counteracting toxic substances begins morbidity, and the performance hair is fluffy and disorderly, lassitude, and appetite depression, major part has the excrement of having loose bowels symptom.
3, the morbidity of each treatment group (I, II, III) and medicine matched group (IV) is slow than positive controls, and symptom is lighter.Through X
2Check, the M ﹠ M of high, medium and low three the treatment groups of ' Sanhuangqinbao ' powder-medicine for chicken, prevention group and QINJUNLING medicine matched group and positive controls be X relatively
2＞3.84 significant differences prove that trial drug and control drug are tangible to the therapeutic effect of chicken colibacillosis and salmonellosis.The sickness rate of three treatment groups and medicine matched group is X relatively
2＜3.84, difference is not remarkable, means that trial drug and control drug have identical curative effect, and result of the test sees the following form in detail:
Artificial onset's therapeutic outcome:
Group is effectively cured for the protection of examination chicken morbidity chicken death chicken survival chicken rehabilitation chicken
A number of elements number of elements number of elements number of elements digit rate rate rate examination I (high dose group) 50 12 5 45 43 42.9 58.3 77.8 test 50 13 6 44 41 38.1 50 66.7 pairs of IV of 50 12 4 46 43 42.9 66.7 66.7 groups of III (low dose group) of II (middle dosage group) (fowl bacterium amount group) 50 13 6 44 40 38.1 50 55.6 according to V (positive control group) 50 21 12 38 29 000 groups of VI (empty map group) 50 // 50 // // protective rate=(positive control group morbidity number-experiment group morbidity number) ÷ control group morbidity number * 100%. Effective percentage=(positive controls invalid number-test group invalid number) ÷ matched group invalid number * 100%.Cure rate=(positive controls not rehabilitation number-test group not rehabilitation number) ÷ matched group is rehabilitation number * 100% not.
4, prophylactic tria without other drug, only prevented with the precious diffusing dispensing at twice of fowl in experimental period; Trial flock and control group are relatively; Do not see difference at spirit, diet desire and incidence; Survival rate improves 5% than control group, and the relative weight gain rate is 105%, illustrates that ' Sanhuangqinbao ' powder-medicine for chicken has protection effect preferably, the alternative chemoprophylaxis of conventional raising, and can improve survivability of chicks and weightening finish.
Five, conclusion
Precious the loosing of Chinese medicine fowl has the obvious treatment effect to artificial onset's chicken colibacillosis, salmonellosis, the alternative chemoprophylaxis of conventional raising, and can improve survival rate and weightening finish.Recommend the treatment consumption in feedstuff, to add 1.5%, 5 days courses of treatment; The prevention consumption adds 1%, 5 days prevention phases, and after drug withdrawal 15-20 days, repeated drug taking 5 days.
The clinical experiment report of ' Sanhuangqinbao ' powder-medicine for chicken prevention infectious bursal disease
' Sanhuangqinbao ' powder-medicine for chicken is a pure Chinese medicinal preparation, and sick and virosis all has certain prevention effect to the various bacteria of chicken.This test is intended to verify the prevention effect of this medicine to infectious bursal disease, and carries the using dosage and the course of treatment.
One, test material:
1, trial drug: ' Sanhuangqinbao ' powder-medicine for chicken (powder), specification 500g/ bag, lot number: 980901, Shandong poultry association provides.
2, control drug: QINJUNLING, specification: the 250g/ bag, lot number: 980126, preventing and treating recommended dose is 0.6% interpolation feedstuff.Shandong Province Laiwu City animal pharmaceutical factory produces, and Shandong poultry association provides.
3, test chicken: 300 of 19 age in days healthy chicks, no infectious bursal disease vaccine immunity history.
4, seed culture of viruses: the die of illness strong poison of fabricius bursa tissue (1: 3) of chicken of infectious bursal disease is that this institute separates preservation.
Two, test method:
1, test chicken grouping: get 300 of test chickens, breeding observing 3 days, treat that the chicken group is stable after, be divided into 6 groups at random, 50 every group.
2, test group design: each test group dosing mode and dosage are as follows:
(1) group: the high dose medicament group, feeding contains the feedstuff of sending out of ' Sanhuangqinbao ' powder-medicine for chicken 2%.
(2) group: middle dose drug group, feeding contains the feedstuff of ' Sanhuangqinbao ' powder-medicine for chicken 1.5%.
(3) group: the low-dose drugs group, feeding contains the feedstuff of ' Sanhuangqinbao ' powder-medicine for chicken 1%.
(4) group: medicine matched group, the feedstuff (recommendation therapeutic dose) of feeding and containing QINJUNLING
(5) group: positive controls, artificial challenge, not administration, the conventional feed of feeding.
(6) group: the blank group, do not infect, not administration, the conventional feed of feeding is with other group isolated rearing.
Each organizes test chicken free choice feeding and drinking-water, and the 5 days laggard pedestrian workers that offer medicine infect.
3, artificial challenge's method: the strong poison of 1 group of-5 groups of artificial challenge's infectious bursal disease tissue, every chicken eye drip, collunarium, drip each 2 of cloacas, amount to 0.25ml.Observed 10 days continuously behind the counteracting toxic substances, incidence, clinical manifestation, death toll respectively organized in record, and cut open and examine the chicken that dies of illness, and observes pathological changes, determines the cause of the death.
4, result's statistics: statistics protective rate, cure rate, effective percentage, survival chicken average weight, each test group is carried out X
2Check.
Three, result of the test:
The chicken group begins to occur clinical symptoms after the artificial challenge is 30 hours, infects beginning death after 48 hours, and the sickness rate of positive controls is 96%, and mortality rate is 66%; And the sickness rate of chemoprophylaxis group is lower than 70%, and mortality rate is lower than 33%, and sickness rate has reduced by 26%, and mortality rate has reduced by 33%.Test statistics the results are shown in following table:
The result of the test of ' Sanhuangqinbao ' powder-medicine for chicken control IBD
| Group | For examination chicken number | Morbidity chicken number | Dead chicken number | Survival chicken number | Survival chicken average weight | Protective rate % | |
| Test group | 1 (high dose group) | 50 | ?30 | 39 | 118.0 | ?37.5 | |
| 2 (middle dosage groups) | 50 | ?32 | 12 | 116.9 | ?33.3 | ||
| 3 (low dose group) | 50 | ?35 | 16 | 34 | 112.3 | ?27.1 | |
| Matched group | 4 (medicine matched groups) | 50 | ?36 | 14 | 36 | 114.7 | ?25.0 |
| 5 (positive controls) | 50 | ?48 | 33 | 17 | 105.4 | ?/ | |
| 6 (blank groups) | 50 | ?0 | 0 | 50 | 126.1 | ?100???? | |
Result between each group is carried out X
2Check: 1,2,3,4 groups to 5 groups X
2Value is respectively 19.64 (＞6.63, difference is extremely remarkable), 17.82 (＞6.63, difference is extremely remarkable), 11.56 (＞6.63, difference is extremely remarkable), 14.49 (＞6.63, difference is extremely remarkable); 3 groups to 4 groups X
2Value is 0.19 (＜3.84, difference is not remarkable); 1,2 groups to 3 groups X
2Value is respectively 1.27 (＜3.84, difference is not remarkable), 0.79 (＜3.84, difference is not remarkable); 1 group to 2 groups X
2Value is 0.06 (＜3.84, difference is not remarkable).
Biology statistical result showed: the difference between chemoprophylaxis group and medicine matched group and the positive controls is all extremely remarkable, show trial drug for the prevention infectious bursal disease effective; Difference is not remarkable between trial drug and the control drug; Difference is not remarkable between each group of trial drug, but combines the full powers weighing apparatus from aspects such as morbidity number, survival chicken number, protective rate and survival chicken average weights, and middle dosage group is better.
Four, conclusion:
This evidence, precious the loosing of Chinese fowl has certain clinical effectiveness to the prevention infectious bursal disease.Recommending preventive dose is 1.5%, spice.Auxiliary treatment when also can be used for falling ill.
The therapeutic effect of ' Sanhuangqinbao ' powder-medicine for chicken is determined by clinical trial.In order to verify that its stable spy does following test:
Test one:
1. test objective t observe this medicine under the normal temperature laboratory condition its stability and shelf-life determines.
2. test method: get same product batch number and be this product of 970419.Seal by every 100g pack, public affairs become 6 degree sample groups, and one bag every group, be positioned over simultaneously under the normal temperature laboratory and preserve, note ventilation, dry, lucifuge.Respectively at 3 months, 6 months, 12 months and 18 months not equal time observe, write down as follows to its character (shape, color, hide) and weight change etc.:
Test two:
1, test objective: observe the stability of this medicine in drying baker.
2, test method: will be that this product of 980901 is divided into 8 sample groups with～product batch number, every group of 100g packs into (adding a cover) in the port grinding bottle, will be wherein 7 groups put into thousand behaviour's casees simultaneously, stay one group and compare.Temperature in the dried case is transferred to 60 ℃ kept 42 days,, its character (shape, color, flavor) and loss on drying etc. are observed, write down as follows every one group of 7 days sample thief:
By above test one and test two, prove " ' Sanhuangqinbao ' powder-medicine for chicken " its stable in properties under the condition in normal temperature laboratory and 60 ℃ of casees.The indoor of drying, ventilation, lucifuge, a year and a half shelf-life are deposited in requirement.
The ' Sanhuangqinbao ' powder-medicine for chicken quality standard
Prescription:
Radix Scutellariae 18 Rhizoma Coptidis 16 Cortex Phellodendris 5
Fructus Gardeniae 16 Radix Angelicae Sinensis 8 Rhizoma Atractylodis Macrocephalaes 7
Radix Et Rhizoma Rhei 5 Fructus Chebulae 6 Radix Paeoniae Albas 8
Cortex Cinnamomi 4 Poria 3 Rhizoma Chuanxiong 4
Method for making: above-mentioned pulverizing medicinal materials, sieve, to mix and collude, packing is promptly.
Character: this product is yellowish-brown toner.
Differentiate: fiber is faint yellow, fusiformis, and wall thickness, the hole ditch is thin.Fibre bundle foresythia, wall are thick slightly, and pit is obvious.Calcium oxalate cluster crystal is dispersed in or is present in the parenchyma cell, and constant to dozens of vertically is arranged in rows, and diameter is approximately to 41um.Calcium oxalate cluster crystal is big, diameter 60-140um.Parenchyma cell is spindle, and wall is slightly thick, and atomic thin oblique cross lamination is arranged, and rubbish shape agglomerate was colourless in irregular minute, meets chloral hydrate liquid and dissolves; Hyphae colorless or light brown, diameter 6-4um.Plant skin stone cell yellow or light brown, how broken, complete person is polygon, rectangle or out-of-shape, and wall thickness has big circular pit, the cell brownish red.The fibre bundle foresythia, peripheral cell contains prism of calcium oxalate, forms crystalline cellulose, and the wall lignify of crystal cell thickens.Needle-like calcium oxalate crystal is tiny, and long 5-32um is filled in the parenchyma cell brokenly.
Check: meet regulation under " Chinese veterinary drug allusion quotation " 90 editions two powder items.
The Chinese medicine of respectively distinguishing the flavor of in the prescription all belongs to pure natural substance, stable in properties.Manufacturing process does not add any chemical substance, does not change its original character after grinding mixing.
Multi-flavor medicine in the prescription has antibacterial mildew-resistant effect.Therefore, its preparation nature is stable.A year and a half shelf-life under normal circumstances.
Specification: 250g/ bag, 500g/ bag
Storage: the dry place of lucifuge preserves.
Embodiment
Prescription
Radix Scutellariae 18 Rhizoma Coptidis 16 Cortex Phellodendris 5
Fructus Gardeniae 16 Radix Angelicae Sinensis 8 Rhizoma Atractylodis Macrocephalaes 7
Radix Et Rhizoma Rhei 5 Fructus Chebulae 6 Radix Paeoniae Albas 8
Cortex Cinnamomi 4 Poria 3 Rhizoma Chuanxiongs 4
At first each flavor Chinese medicine (decoction pieces) is measured to pack to seal-case through the smalls that is up to the standards-is ground into-after stirring-sterilize by prescription requirements and get final product.
Function with cure mainly: heat-clearing and toxic substances removing, dampness-eliminating and dysentery-stopping, invigorating the spleen to arrest diarrhea.Cure mainly the white scour of chicken,
Colibacillosis, fowl cholera, chicken bursa fabricic.
Usage and consumption: mixed feeding therapeutic dose: add this product 1500-2000 (1.5-2% mixed feeding) in every 100kg feedstuff.Preventive dose: 1% mixed feeding, 3-5 days is a course of treatment.
Claims (5)
1. ' Sanhuangqinbao ' powder-medicine for chicken is characterized in that it is the medicament of being made by the following weight proportion raw material
Radix Scutellariae 10-20 Rhizoma Coptidis 10-20 Cortex Phellodendri 4-10
Fructus Gardeniae 10-20 Radix Angelicae Sinensis 4-10 Rhizoma Atractylodis Macrocephalae 1-6
Radix Et Rhizoma Rhei 4-10 Fructus Chebulae 4-10 Radix Paeoniae Alba 4-10
Cortex Cinnamomi 1-6 Poria 4-6 Rhizoma Chuanxiong 2-6
2. ' Sanhuangqinbao ' powder-medicine for chicken according to claim 1 is characterized in that
Radix Scutellariae 15-20 Rhizoma Coptidis 10-18 Cortex Phellodendri 4-8
Fructus Gardeniae 14-20 Radix Angelicae Sinensis 6-10 Rhizoma Atractylodis Macrocephalae 3-6
Radix Et Rhizoma Rhei 4-8 Fructus Chebulae 6-10 Radix Paeoniae Alba 6-10
Cortex Cinnamomi 3-6 Poria 2-4 Rhizoma Chuanxiong 4-6
3. ' Sanhuangqinbao ' powder-medicine for chicken according to claim 1 is characterized in that
Radix Scutellariae 18 Rhizoma Coptidis 16 Cortex Phellodendris 5
Fructus Gardeniae 16 Radix Angelicae Sinensis 8 Rhizoma Atractylodis Macrocephalaes 7
Radix Et Rhizoma Rhei 5 Fructus Chebulae 6 Radix Paeoniae Albas 8
Cortex Cinnamomi 4 Poria 3 Rhizoma Chuanxiong 4
4. according to claim 1,2 or 3 described ' Sanhuangqinbao ' powder-medicine for chicken, it is characterized in that said medicament is a powder.
5. ' Sanhuangqinbao ' powder-medicine for chicken according to claim 4 is characterized in that by the prescription requirement, and every medicine is ground into 120 purpose fine powders, stirs, and sterilizes 10-20 minute, makes powder.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN99112359A CN1089000C (en) | 1999-08-11 | 1999-08-11 | 'Sanhuangqinbao' powder-medicine for chicken |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN99112359A CN1089000C (en) | 1999-08-11 | 1999-08-11 | 'Sanhuangqinbao' powder-medicine for chicken |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1284372A true CN1284372A (en) | 2001-02-21 |
| CN1089000C CN1089000C (en) | 2002-08-14 |
Family
ID=5275674
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN99112359A Expired - Fee Related CN1089000C (en) | 1999-08-11 | 1999-08-11 | 'Sanhuangqinbao' powder-medicine for chicken |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1089000C (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102895374A (en) * | 2012-10-30 | 2013-01-30 | 天津集合科技有限公司 | Chinese medicinal composition for treating green goose pseudomonas aeruginosa |
| CN103041235A (en) * | 2013-01-22 | 2013-04-17 | 孙凤来 | Zhuangyuan antidiarrheal powder |
| CN103372086A (en) * | 2012-04-23 | 2013-10-30 | 德州和膳生态农业有限公司 | Traditional Chinese medicine-based health care and epidemic prevention method for laying hen breeding |
| CN103768502A (en) * | 2012-10-24 | 2014-05-07 | 天津中敖生物科技有限公司 | Traditional Chinese medicine composition for treating cow acute enteritis and preparation method of traditional Chinese medicine composition |
| CN107551215A (en) * | 2017-03-20 | 2018-01-09 | 安徽天安生物科技股份有限公司 | It is a kind of to improve kind of a duck laying rate, the Chinese prescription of the anti-lopsided egg of production |
| CN110522880A (en) * | 2018-05-24 | 2019-12-03 | 河南炎黄生物工程有限公司 | A kind of Chinese medicine composition for preventing and treating laying hen mycotoxicosis, Chinese medicine microecological preparation and preparation method thereof |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB9013859D0 (en) * | 1990-06-21 | 1990-08-15 | Ici Plc | Zeolites |
| CN1124649A (en) * | 1995-08-21 | 1996-06-19 | 广西中兽药厂 | Compound preparation for resisting white diarrhea of poultry and domestic animals |
| CN1125618A (en) * | 1995-09-14 | 1996-07-03 | 吉林省生物制品厂生物药品研究所 | Oral liquid for treatment of chicken's Newcastle disease and Fabricius bursa disease |
-
1999
- 1999-08-11 CN CN99112359A patent/CN1089000C/en not_active Expired - Fee Related
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103372086A (en) * | 2012-04-23 | 2013-10-30 | 德州和膳生态农业有限公司 | Traditional Chinese medicine-based health care and epidemic prevention method for laying hen breeding |
| CN103768502A (en) * | 2012-10-24 | 2014-05-07 | 天津中敖生物科技有限公司 | Traditional Chinese medicine composition for treating cow acute enteritis and preparation method of traditional Chinese medicine composition |
| CN102895374A (en) * | 2012-10-30 | 2013-01-30 | 天津集合科技有限公司 | Chinese medicinal composition for treating green goose pseudomonas aeruginosa |
| CN102895374B (en) * | 2012-10-30 | 2014-08-13 | 天津集合科技有限公司 | Chinese medicinal composition for treating green goose pseudomonas aeruginosa |
| CN103041235A (en) * | 2013-01-22 | 2013-04-17 | 孙凤来 | Zhuangyuan antidiarrheal powder |
| CN107551215A (en) * | 2017-03-20 | 2018-01-09 | 安徽天安生物科技股份有限公司 | It is a kind of to improve kind of a duck laying rate, the Chinese prescription of the anti-lopsided egg of production |
| CN110522880A (en) * | 2018-05-24 | 2019-12-03 | 河南炎黄生物工程有限公司 | A kind of Chinese medicine composition for preventing and treating laying hen mycotoxicosis, Chinese medicine microecological preparation and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1089000C (en) | 2002-08-14 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102356823B (en) | Chinese herbal medicine additive for reducing stress reaction of a weaned pig and pig feed thereof | |
| CN102038781B (en) | Pharmaceutical composition for inhibiting bacteria and preparation method and application thereof | |
| CN101829211B (en) | Fish compound traditional Chinese medicinal preparation and preparation method thereof | |
| CN101836691A (en) | Chinese medicinal herb feed additive | |
| CN109674778B (en) | Lipid antibacterial composition, extraction method, detection method and application | |
| CN104800819B (en) | A kind of Chinese medicine preparation for treating checken pest | |
| CN1927303A (en) | Chinese medicine for treating chicken respiratory tract disease | |
| CN103877229B (en) | A kind of compound Chinese medicine extract and application thereof preventing and treating an avian colibacillosis | |
| CN102671011B (en) | Traditional Chinese medicine compound powder for preventing and treating yellow and white scour of piglets and preparation method thereof | |
| CN1089000C (en) | 'Sanhuangqinbao' powder-medicine for chicken | |
| CN102872307A (en) | Traditional Chinese medicine oral liquid for preventing and treating virus diseases of poultry and method for preparing traditional Chinese medicine oral liquid | |
| CN106173450A (en) | Improve compound feed additive and the feedstuff of pigeon immunity | |
| CN103404709B (en) | Feed additive produced from waste liquid and dregs in pharmaceutical factory and preparation method of feed additive | |
| CN102579622B (en) | Chinese herbal preparation for treating haemophilusparasuis and preparation method thereof | |
| CN101085037A (en) | Traditional Chinese medicine preparation for animals and preparation method thereof | |
| CN101134074B (en) | Compound traditional Chinese powder medicine for treating chronic mastitis for cows | |
| CN106858067A (en) | Livestock and poultry nonreactive growth promotion compound preparation and its production and use | |
| CN103417653B (en) | Veterinary-use compound pure Chinese herbal medicine for treating coccidium and preparation method of oral liquid of same | |
| CN1148217C (en) | Chinese medicine for treating chronic bronchitis | |
| CN110354194A (en) | Chinese medicine composition and preparation method thereof for preventing and treating porcine mycoplasmal pneumonia | |
| CN1899553A (en) | Paste for treating dental hyperesthesia and its preparing method | |
| CN106135667A (en) | The poultry of a kind of alternative antibiotic local flavor zacate additive and preparation method thereof | |
| CN109845921A (en) | A kind of functional bait and preparation method thereof for preventing and treating fancy carp stigmatosis | |
| CN105796928A (en) | Traditional Chinese medicine composition for treating avian coccidiosis | |
| CN104606627B (en) | A kind of pharmaceutical composition for treating grice diarrhoea and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20020814 Termination date: 20090911 |