CN102872307A - Traditional Chinese medicine oral liquid for preventing and treating virus diseases of poultry and method for preparing traditional Chinese medicine oral liquid - Google Patents
Traditional Chinese medicine oral liquid for preventing and treating virus diseases of poultry and method for preparing traditional Chinese medicine oral liquid Download PDFInfo
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Abstract
The invention discloses traditional Chinese medicine oral liquid for preventing and treating virus diseases of poultry and a method for preparing the traditional Chinese medicine oral liquid, and relates to a traditional Chinese medicine for treating diseases of poultry and a method for preparing the traditional Chinese medicine. The traditional Chinese medicine oral liquid comprises 40g of astragalus, 40g of radix scutellariae, 30g of radix isatidis, 30g of forsythia, 30g of radix bupleuri, 25g of sanguisorba officinalis, 25g of licorice, 25g of garlic and 5g of toad venom. The method includes weighing medicinal materials except for the toad venom according to the proportion to obtain a mixture, adding the mixture into water for warm soaking, decocting the mixture twice, filtering, centrifugally separating or settling and filtering to obtain filtrate, concentrating the filtrate until the density of thick paste ranges from 1.20 to 1.25 and taking out the thick paste; and adding ethanol into the thick paste to obtain a mixture, refrigerating the mixture, allowing the mixture to stand, depositing insoluble impurities, filtering to obtain supernatant, refrigerating the supernatant, allowing the supernatant to stand, recovering the ethanol, adding the prescription amount of the toad venom into the supernatant to obtain a mixture, adding the balance water into the mixture so as to obtain 250ml of liquid mixture, filling the liquid mixture in a package, sterilizing the liquid mixture in the package to obtain the oral liquid. The oral liquid can improve anti-virus capacity of poultry bodies and prevent secondary infection. The solving scheme for technical quality control is reasonable, and accordingly the preparation has the advantages that the traditional Chinese medicine oral liquid is safe, reliable and non-toxic, and is free of residuals and convenient to use, and the process technology is simple and feasible.
Description
Technical field
The present invention relates to Chinese medicine of a kind of fowl diseases of managing a household and preparation method thereof, particularly relate to a kind of Chinese medicine oral liquid of preventing and treating gal virus class disease and preparation method thereof.
Background technology
Along with developing rapidly of aquaculture, the aviculture disease popularity is day by day frequent, and aviculture is endured bacterial drug resistance to the fullest extent and strengthened, and the diseases prevention and treatment cost is huge the puzzlement such as to increase, and causes raiser's interests to guarantee.Use which type of veterinary drug in the animal feeding process, its quality is played an important role.In recent years China's livestock products outlet seriously is obstructed, and residual the exceeding standard of the meat traditional Chinese medicine thing that the veterinary drug improper use causes is one of the main reasons.Market at home, drug residue has suppressed the steady growth of carnivorous consumption to a certain extent, and the green safety meat product is very popular, and the use of Chinese medicine in breeding process provides good recipe for solving drug residue.To careful use veterinary drug problem, China has also promulgated a series of regulations, but in actual production illegal use and the abuse of antibiotics problem very serious, people and animals' medicine is used with also very general.For a long time, Western medicine is high with its curative effect, instant effect is used at the wide model of plant, following serious drug resistance, drug residue and pollution problem.Move in recent years and protect Industry Quick Development, just towards curative effect is high, drug resistance is low, effect is lasting, the future development of noresidue, the antibiotics additive will be replaced by Chinese herbal medicine and growth promotion viable bacteria gradually owing to the residual and toxic action of medicine.Domestic veterinary drug market is antibiotic, the anti virus herb product development has become focus.Especially export broiler, concerned countries regulation, many antibiotic ban use of at feeding period, or to medicine is residual strict demand are arranged.Therefore Western medicine is restricted day by day in the use of field of veterinary at present, and the Chinese medicine extract antibiosis and antiviral functions is powerful, and without toleration and toxic and side effects, is day by day paid much attention to by the veterinary drug production field.
Along with intensive, the large-scale development of modernization poultry, chicken group's stocking density is increasing, infectious disease; particularly viral infectious in case occur, often can bring huge economic loss to production; especially the stronger infectious disease of those transmission capacities; such as ND, AI, IBD, IB, ILT etc., can be in the chicken group after the generation rapid spread, sometimes have little time to take measures to cause the large tracts of land diffusion; therefore; for viral disease, still to implement the principle of " put prevention first, anti-overweighting controlled ".Under this prerequisite, treatment as a kind of urgent remedial measure after falling ill, is reduced the economic loss that causes thus.2005 the end of the year Ministry of Agriculture forbid the antiviral Western medicine in edible animal comprehensively, in the aviculture new antiviral drugs demand urgently the exploitation.
Herbal medicine itself has natural sex, multifunctionality, has no side effect, without characteristics such as Drug resistance, can effectively reduce the drug residue in the animal products.The antibacterial and antiviral drug that from Chinese medicine, obtains, effective component content is high, the anti-bacteria and anti-virus height of tiring, easy to use, medicament sources is abundant, comparing with production of antibiotics, its production technology is simple, cost is low, environmentally safe, can improve the immunologic function of chicken body, strengthen the immune effect of vaccine, improve chicken group's resistance and survival rate, have considerable economic benefit, therefore good application value is arranged in production reality.After Chinese medicine is absorbed by body, can be converted into and have antibacterial and material antivirus action, improve body self resistance against diseases and nonspecific immunity function, play good preventive and therapeutic effect.Traditional Chinese Medicine Anti virus function mechanism mainly refers on the one hand directly the killing of virus, and blocking virus to Normocellular absorption, the link such as penetrate, copy and reach antiviral purpose.The activity of the cellular immunization by the growth of Promote immunity organ, the humoral immunization that strengthens cytophagous phagocytic activity, enhancing body, enhancing body, inducement interferon, enhancing natural killer cell, the untoward reaction that improves body due to the virus and the immune defense system that the pathological changes equal excitation is transferred body are brought into play antivirus action indirectly on the other hand, this approach is particularly important, the organic conception base therapy thought that more meets Chinese veterinarian's strengthening vital QI to eliminate pathogenic factors, the control chicken viral diseases, research and development new medicine preparation has wide prospect.Therefore, the effect of herbal medicine in its feeding is also just further important.
Summary of the invention
The object of the present invention is to provide a kind of Chinese medicine oral liquid of preventing and treating gal virus class disease and preparation method thereof, oral liquid prescription adapts to the needs in market, can improve the poultry antiviral ability, prevents secondary infection.Processing quality control solution is reasonable, has realized that preparation safety is reliable, nonhazardous, noresidue, easy to use, the characteristics that Technology is simple.
The objective of the invention is to be achieved through the following technical solutions:
A kind of Chinese medicine oral liquid of preventing and treating gal virus class disease, the prescription proportioning of described oral liquid is: Radix Astragali 40g, Radix Scutellariae 40g, Radix Isatidis 30g, Fructus Forsythiae 30g, Radix Bupleuri 30g, Radix Sanguisorbae 25g, Radix Glycyrrhizae 25g, Bulbus Allii 25g, Venenum Bufonis 5g.
A kind of preparation method of preventing and treating the Chinese medicine oral liquid of gal virus class disease, the method comprises following process:
Take by weighing each medical material except Venenum Bufonis in the prescription ratio, add water temperature and soaked 1 hour, decoct again 2 times, decocted 1 hour for the first time, decocted 40 minutes for the second time, filter, merging filtrate, centrifugalize or sedimentation are taken out when filtrate is concentrated into thick paste density 1.20-1.25 after filtering; Add ethanol, make to contain the alcohol amount and reach 65%～70%, cold preservation was left standstill 24 hours, then make the insoluble impurities precipitation, get its supernatant liquid filtering, take out and filter, cold preservation was left standstill 24 hours, Recycled ethanol, add the recipe quantity Venenum Bufonis and add water move to 250ml, fill, sterilization, namely get the oral liquid that appearance character is brown color clear liquid.
Advantage of the present invention and effect are:
1. the present invention selects technique centered by the characteristic of effective components of Chinese medicinal, chemical property in conjunction with active ingredients of plants is strictly organized technique, the oral liquid effective component content that extract is made is high, the anti-bacteria and anti-virus height of tiring finally makes the anti-bacteria and anti-virus of finished product tire to equal even is higher than tiring of used medical material.Can directly substitute the anti-bacteria and anti-virus medical material in the Chinese medicine, because the Chinese medicine extract absorbance is very high, so the curative effect of 1 gram extract greater than 20 gram raw medicinal herbs powder, has improved the technology content of product, reduce small product size; The antibiotic complicated of Chinese herbal medicine, antibacterial be difficult to effectively identification and produce toleration, be used for strengthening the curative effect of Western medicine, and remedy the easy tolerific shortcoming of Western medicine; Can strengthen the product toleration thereby mix with Western medicine, and increase its curative effect; And can make separately the anti-bacteria and anti-virus preparation, be used for the treatment of the infectious disease that all kinds of bacterial viruses cause.
2. prescription of the present invention is that the scientific research group is founded alone according to years'experiences, and pure Chinese medicine noresidue is safe, and noresidue, has no side effect.By using said preparation in the cultivation, omnidistance Chinese herb on the prevention has been received good curative effect, Chinese herb on the prevention dispensing cost is higher than Western medicine, although the disposable dispensing cost of Chinese medicine is high, but the time of keeping is more much longer than Western medicine, is not higher than Western medicine or is lower than the Western medicine expenses for medicine from average every chicken of a collection of chicken of integral body.This reasonable recipe, treating both the principal and secondary aspects of a disease has good prevention, therapeutical effect to viral infection, and life-time service Chinese medicine can improve chicken body premunition, has no drug resistance and the off-drug period.
3. the used medicine of the present invention has and strengthens or regulate the effects such as body non-specific immunity and specific immune function, antiinflammatory, antiviral, enhancing digestive system function, and the body constitution of chicken is strengthened.Show by experiment in the layer breeding process use this Chinese medicine oral liquid prevention drinking-water after, the matched group chicken basal diet of feeding; Chicken day egg production, egg size, feed intake are respectively organized in omnidistance observational record all increase, and sickness rate, broken egg number, mortality rate all have decline.Test group chicken experimental period total earnings contrast group increases average every chicken and takes in 1.03-2.20 unit more; Economic benefit improves 13.0%-26.6%.The contrast test that preparation of the present invention and antibiotics use in the feeding of broiler process, result show that broiler growth, survival rate and feed conversion rate improve 5.1%, 8.2% and 4.5% than antibiotic group respectively.Proof has no adverse effects to the growth of broiler chicken, the test chicken honey stomach, and feather is smooth-going, and weight ratio is more even, is in a good state of health, and improves survival rate more than 20%.Fur color and luster chicken group's spiritual outward appearance, mental status, the uniformity, chicken all obviously is better than matched group.
The specific embodiment
The present invention is described in detail below in conjunction with embodiment.
Its prescription is: Radix Astragali 40g, Radix Scutellariae 40g, Radix Isatidis 30g, Fructus Forsythiae 30g, Radix Bupleuri 30g, Radix Sanguisorbae 25g, Radix Glycyrrhizae 25g, Bulbus Allii 25g, Venenum Bufonis 5g.Take by weighing each medical material except Venenum Bufonis in the prescription ratio, add water temperature and soaked 1 hour, decoct again 2 times, decocted 1 hour for the first time, decocted 40 minutes for the second time, filter, merging filtrate, centrifugalize or sedimentation are taken out when filtrate is concentrated into thick paste density 1.20-1.25 after filtering.Add ethanol, make to contain the alcohol amount and reach 65%～70%, cold preservation was left standstill 24 hours, then make the insoluble impurities precipitation, get its supernatant liquid filtering, take out and filter, cold preservation was left standstill 24 hours, Recycled ethanol, add the recipe quantity Venenum Bufonis and add water move to 250ml, fill, sterilization, namely get the oral liquid that appearance character is brown color clear liquid.
Taking test situation illustrates as follows:
1. the therapeutic test of Nephropathogenic infectious bronchitis:
Nephropathogenic infectious bronchitis (being called for short the kidney biography props up) is that the kidney sexually transmitted disease (STD) of infectious bronchitis becomes, and is a kind of acute, the height contagious disease.This disease sickness rate is high, and mortality rate is high, is one of Important Infectious Diseases that affects poultry husbandry.Certain raiser brings 10 dead chickens medical.Introduce its 5000 of broiler of cultivation, brood at same hen house, to an other hen house turn the group half, it is lower to turn group rear hen house temperature, respiratory symptom appears in the chicken group after 2 days, mainly take the sneeze of coughing as main.Fed 3 days tylosins, respiratory tract obviously alleviates, and dead chicken can occur successively, and the watery diarrhea phenomenon is arranged.Spend 2 days, dead more than 50 of every day, chicken group spirit is depressed, and diarrhoea is serious, and amount of drinking water increases, and feed intake obviously reduces.According to the epidemic characteristic clinical symptom, infectious bronchitis of chicken can be divided into breathing pattern, the kidney type, Glandular Stomach Type and anomaly: breathing pattern cooperates the upper respiratory tract antibiotic medicine with the Chinese medicine antiviral agents, begin to use Chinese medicine oral liquid of the present invention during with slight cough phenomenon, see the result that manifest symptom alleviates at second day; The kidney type falls apart with Chinese medicine five skins and reaches vitamin A, vitamin C, and cooperates Chinese medicine oral liquid drinking-water of the present invention; The general morbidity of Glandular Stomach Type relatively early prevents primary disease to occur for the chicken group Chinese medicine oral liquid of the present invention of throwing something and feeding, and with the loose cooperation of epidemic disease health intestinal medicine.Strengthen simultaneously feeding and management, note coop ventilation, prevented from squeezing, note moisturizing, in time immunity inoculation.
2. the control of infectious bursal disease test:
Support 10000 broiler, occurred dead in 19 days.Local veterinary is judged as the fabricius bursa immuning failure.Supply the examination chicken to select respectively natural occurrence second day chicken group's meat sold on the market fowl disease chicken, select at random disease chicken, dead chicken, dissection and laboratory diagnosis all to meet the chicken group of bursal disease pathological change feature for supplying to try chicken.Select to be more or less the same for the sick chicken body weight of examination, be the morbidity chicken group of same house as far as possible.Extend trial has 100,000 cover meat kind chickens in addition with chicken, gets 4000 kind chickens of doing the Seedling front and back for examination.
Use Chinese medicine oral liquid of the present invention, be used for the control of bursal disease of chicken and the enhancing of immunity, one bottle of 800 chicken that clinical use amount provides by local veterinary, every bottle of 500 ml, Chinese medicine oral liquid is used in conjunction 3 days; Fabricius bursa yolk sac antibody 1ml/, intramuscular injection; Be used kidney-reinforcing drug, secondary infection medicine.
(1) test grouping: be diagnosed as 400 of bursal disease broiler in the chicken house taking-up, for Chinese medicine oral liquid is treated 1 group; Take out again and be diagnosed as 400 of bursal disease broiler, be 2 groups of fabricius bursa yolk sac treatment contrasts; Blank 100 are 3 groups of contrasts.Feeding and management environment, condition, mode are similar.
(2) medication: test 1 group and adopt Chinese medicine oral liquid for the examination chicken, one bottle of 800 chicken is used in conjunction 3 days, and each is once sooner or later; Be used simultaneously kidney-reinforcing drug, and prevent the antimicrobial drug of secondary infection.
Test 2 groups of matched group chickens and only adopt fabricius bursa yolk sac antibody 2ml/.Be used equally kidney-reinforcing drug, and prevent the antimicrobial drug of secondary infection.
Testing 3 groups of blank groups adopts the antibiotics medicine to cooperate kidney-reinforcing drug, conventional treatments.
(3) result of the test: result of the test shows that the Chinese medicine oral liquid test group is similar to the effect of fabricius bursa yolk sac Antybody therapy group, and (IBD) has good therapeutic effect to infectious bursal disease.Three days of medication can both onset, just can control the state of an illness in 4～5 days, sick chicken stops death, survival rate reaches more than 89%; Wherein the Chinese medicine oral liquid test group is up to 89%, and high-immunity yolk capsule Antybody therapy group survival rate reaches 91 %; And the matched group survival rate of antibiotics conventional therapy only has 30%, difference highly significant (P＜0.01), and result of the test is as shown in table 1.
Table 1 contrast therapy result of the test
| Group | For examination chicken (plumage) | 5 daily mortalities after the morbidity | 10 daily mortalities after the morbidity | The survival number | Survival rate (%) |
| The Chinese medicine oral liquid treatment group | 400 | 40 | 3 | 357 | 89 |
| High-immunity yolk capsule Antybody therapy group | 400 | 35 | 1 | 364 | 91 |
| The antibiotic therapy group | 100 | 48 | 22 | 30 | 30 |
(4) clinical expansion range of application experiment
On the basis by preliminary examination, the Chinese medicine oral liquid treatment is suitably adjusted, and carried out clinical expansion application experiment in fowl industry, chicken farm.
Success of the test prevents simultaneously plant's neopathy broiler and the broiler of not falling ill afterwards and treats.The 4h that before medication, cuts off the water, according to above-mentioned medication, free drink-service.2600 chickens of chicken that do not fall ill, 47 of prevention sequela, protective rate reaches 98.2%; 1200 of chickens of morbidity are treated, and cure 1120, and cure rate reaches 93%.
Fowl industry 100,000 cover meat kind chickens.Doing the Seedling front and back, Chinese medicine oral liquid is drunk by one bottle of 2000 chicken at every turn, and chicken group antibody horizontal is very good, the length of holding time.When laying eggs the peak, drink by one bottle of 2000 chicken, be not easy morbidity when laying eggs the peak, the peak of the laying eggs length of holding time.
3. to the therapeutic test of Newcastle disease:
(1) laboratory animal: commodity egg is diagnosed as newcastle disease and naturally sick chicken that untreated is crossed according to chicken clinical symptoms and epidemiology.
(2) experimental technique: this test is divided into five groups of A, B, C, D, E, every group of 200 sick chickens at random.The A group is only treated 0.5g/ with Chinese powder medicine, 1 day disposable spice; The B group uses Chinese herbal medicament oral liquid drinking agent, 1ml/ only to add in the drinking-water, and 1 day 2 times, control water is 2 hours before each dosing, has drunk free choice feeding in 2～3 hours; C group gene interferon therapy uses by producer's explanation, and cooperates the Chinese herbal medicament oral liquid drinking agent; The D group is the gene interferon therapy, uses by explanation; The E matched group after the morbidity, is not given any medicine.
(3) drug effect criterion: medication is the spirit improvement after 3 days, and diet increases, and the death of chicken is under control, and the state of an illness has obtained alleviation, is judged to effectively; Within 5 days, with clinical symptom disappearance, drink water, the person of returning to normal of searching for food is judged to clinical cure; After 3 days spirit have no take a turn for the better be judged to died invalid.Dispensing 5～7 d are a course for the treatment of.Continue to observe 3～5 d after the drug withdrawal, binding of pathological cuts open inspection and takes a sample test the result and determine prevention effect, statistics control effective percentage again.Observe simultaneously the level of production, confirm the comprehensive benefit of said preparation.To the person of being in a bad way, taking liquid gavages, suitably dosage.
(4) test results and analysis see Table 2
The therapeutic effect statistics of table 2 pair natural occurrence chicken
As seen from Table 12: the front 3d after dispensing, the mortality of chicken is larger, illustrate that this sick fatality rate is larger, but obtained effective control behind the 3d, and behind the 7d that throws something and feeds, do not have dead chicken all to fully recover, illustrate that Chinese medicine oral liquid of the present invention has good control and therapeutic effect to newcastle disease.From the mortality rate of rear 4d, extremely significantly be lower than matched group with the chicken group's for the treatment of by Chinese herbs mortality rate, and best with the Chinese medicine oral liquid of the present invention effect of drugs of throwing something and feeding.
The result shows in the medicine contrast test, and Chinese medicine oral liquid ligand of the present invention obviously is better than using separately the gene interferon therapy because this disease of interferon therapy has significant effect.The preventive dosage administration time is that this locality is just popular in large tracts of land generation virosis, chicken is in specific Sensitivity age, the Blank immunization phase, bacterial disease occurs prevent virosis mixed infection.Therapeutic dispensing administration time is at best, the urgent vaccination of the medication prodromal period of disease effect or injection yolk antibody after 48 hours, chicken row's yellow green feces and when agnogenio, when serious immunoreation has occured immunity inoculation.
Impact on Egg Production of Laying Hens: the egg number that records every group of every day during the medication.This result of the test uses the treatment by Chinese herbs group generally through the 3d transference cure, and drinking-water, diet are normal, and growth promoter is without significant difference, the laying rate bottom out.Test shows: Chinese medicine oral liquid does not affect laying eggs of laying hen, has the good result that the egg production of preventing descends.
4. selection quality standard
Adopt thin layer chromatography (TLC) method to identify medical material.
(1) discriminating of Radix Bupleuri: the preparation of standard control solution: get saikoside a reference substance, saikoside d reference substance, add methanol and make the mixed solution that every 1ml contains 0.5mg, product solution is for subsequent use in contrast.
The preparation of contrast liquid: get Radix Bupleuri control medicinal material 0.5g, add methanol 20ml, supersound process 10 minutes filters, and filtrate is concentrated into about 5ml, makes control medicinal material solution.
The preparation of need testing solution: get compound recipe solution 5 mL to be measured, evaporate to dryness moves in the conical flask, adds water 30 mL, heats 30 min, filters, and filtrate evaporate to dryness residue adds methanol 20 mL dissolving, and is for subsequent use.
The preparation of negative fluid: get the compound recipe leachate 0.5mL that removes Radix Bupleuri, processing method is the same.
Algoscopy: according to the thin layer chromatography test, draw each 5 μ l of above-mentioned three kinds of solution, put respectively on same silica gel g thin-layer plate, take ethyl acetate, alcohol and water (8: 2: 1) as developing solvent, launch, take out, dry, spray is with 40% sulfuric acid solution of 2% paradime thylaminobenzaldehyde, and it is clear to be heated to the speckle colour developing at 60 ℃.
Measurement result: put under daylight and the ultra-violet lamp (365nm) and inspect.In the test sample chromatograph, with control medicinal material and the corresponding position of reference substance chromatograph on, speckle or the fluorescence speckle of aobvious same color.Negative controls is disturbed without corresponding speckle.
(2) discriminating of Radix Sanguisorbae medical material: the preparation of standard control solution: get the gallic acid reference substance, add methanol and make the solution that every 1ml contains 0.5mg, product solution is for subsequent use in contrast.
The preparation of contrast liquid: get Radix Sanguisorbae powder 2g, add water 50ml, boiled 30 minutes, let cool, centrifugal 10 minutes, get supernatant, extract 2 times each 15ml with the ether jolting that hydrochloric acid is saturated, merge ether solution, volatilize, residue adds methanol 1ml makes dissolving, makes control medicinal material solution.
The preparation of need testing solution: get compound recipe solution 5 mL to be measured, evaporate to dryness moves in the conical flask, adds water 30 mL, heats 30 min, filters, and filtrate evaporate to dryness residue adds methanol 20 mL dissolving, and is for subsequent use.
The preparation of negative fluid: get the compound recipe leachate 0.5mL that removes Radix Sanguisorbae, processing method is the same.
Algoscopy: according to the thin layer chromatography test, draw each 2 μ l of above-mentioned three kinds of solution, put respectively on same silica gel g thin-layer plate take sodium carboxymethyl cellulose as adhesive, take toluene (using water saturation)-ethyl acetate-formic acid (6: 3: l) as developing solvent, launch, take out, dry, spray is with 1% ferric chloride alcoholic solution.
Measurement result: put under daylight and the ultra-violet lamp (365nm) and inspect.In the test sample chromatograph, with control medicinal material and the corresponding position of reference substance chromatograph on, speckle or the fluorescence speckle of aobvious same color.Negative controls is disturbed without corresponding speckle.
(3) discriminating of Venenum Bufonis medical material: the preparation of standard control solution: get bufogenin reference substance, cinobufagin reference substance, add ethanol and make respectively the solution that every 1ml contains 1mg, product solution is for subsequent use in contrast.
The preparation of contrast liquid: get Venenum Bufonis powder 0.2g, add ethanol 10ml, reflux 30 minutes filters, and filtrate is put in the 10ml measuring bottle, adds ethanol to scale, makes control medicinal material solution.
The preparation of need testing solution: get compound recipe solution 5 mL to be measured, evaporate to dryness moves in the conical flask, adds water 30 mL, heats 30 min, filters, and filtrate evaporate to dryness residue adds methanol 20 mL dissolving, and is for subsequent use.
The preparation of negative fluid: get the compound recipe leachate 0.5mL that removes Venenum Bufonis, processing method is the same.
Algoscopy: according to the thin layer chromatography test, draw each 10 μ l of above-mentioned 5 kinds of solution, put respectively on same silica gel g thin-layer plate, take cyclohexane extraction-chloroform-acetone (4: 3: 3) as developing solvent, launch, take out, dry, spray is with 10% ethanol solution of sulfuric acid, and it is clear to be heated to the speckle colour developing.
Measurement result: in the test sample chromatograph, with the corresponding position of control medicinal material chromatograph on, the speckle of aobvious same color; With the corresponding position of reference substance chromatograph on, an aobvious identical green and punctation.Negative controls is disturbed without corresponding speckle.
5. feed the specific requirement of controlling:
Specification: every bottle of 100ml.
(1) prophylactic: 100 milliliters of this product are watered 200 kilograms, and whole day is freely drunk.Also can the twice concentrated administration of upper and lower noon drink, drank continuously 3～5 days.When epidemic situation is occurred frequently, every usefulness 5 days, interval 5 days recycles.
(2) medicine for treatment: 100 milliliters of this product are watered 100 kilograms, and whole day is freely drunk.Also can the twice concentrated administration of upper and lower noon drink, drank continuously 4～5 days.
(3) the individual medication of serious symptom: adopt the drip administration, 0.1 milliliter of-0.2 milliliter/kg body weight, chicken is 2 droplets/time in two ages in week, about 1500/100 milliliters; Three, all around age 3 droplets/time of chickens, about 1000/100 milliliters; All around age 4 droplets/time of above chickens, about 750/100 milliliters; Every day 2 times, be used in conjunction 3 days.
The present invention has heat-clearing and toxic substances removing, benefiting QI for activating blood circulation, and relieving cough and asthma, the function of spleen invigorating dysentery relieving can enhancing human body immunity power, improves the resistance to disease, strengthens the digestive system effect and promote the poultry growth, and with collaborative use of vaccine auxiliary potentiation is arranged.Some viral diseases such as the present invention prevention and treatment newcastle disease, infectious bursal disease, infectious bronchitis, infectious laryngotracheitis, bird flu cause: hyperpyrexia, cough, dyspnea, shed tears, flow the symptoms such as nose liquid, lethargy are depressed, diarrhoea, feed intake minimizing, growth retardation, and the enteritis that antibacterial and virus mixed infection are caused, diarrhoea etc. also have good curative effect.
Claims (2)
1. a Chinese medicine oral liquid of preventing and treating gal virus class disease is characterized in that, the prescription proportioning of described oral liquid is: Radix Astragali 40g, Radix Scutellariae 40g, Radix Isatidis 30g, Fructus Forsythiae 30g, Radix Bupleuri 30g, Radix Sanguisorbae 25g, Radix Glycyrrhizae 25g, Bulbus Allii 25g, Venenum Bufonis 5g.
2. preparation method of preventing and treating the Chinese medicine oral liquid of gal virus class disease, it is characterized in that, the method comprises following process: take by weighing each medical material except Venenum Bufonis in the prescription ratio, add water temperature and soaked 1 hour, decoct 2 times again, decocted 1 hour for the first time, decocted 40 minutes for the second time, filter merging filtrate, centrifugalize or sedimentation are taken out when filtrate is concentrated into thick paste density 1.20-1.25 after filtering; Add ethanol, make to contain alcohol amount and reach 65%～70%, cold preservation was left standstill 24 hours, then made the insoluble impurities precipitation, get its supernatant liquid filtering, take out and filter, cold preservation was left standstill 24 hours, Recycled ethanol, add the recipe quantity Venenum Bufonis and add water move to 250ml, fill, sterilization namely gets the oral liquid that appearance character is brown color clear liquid.
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| CN103191240A (en) * | 2013-04-17 | 2013-07-10 | 山东省农业科学院家禽研究所 | Traditional Chinese veterinary medicine oral solution for preventing and treating viral diseases of poultry |
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| CN104922401A (en) * | 2015-05-22 | 2015-09-23 | 陶建壮 | Medicine for preventing and treating chicken pest |
| CN106038919A (en) * | 2016-07-14 | 2016-10-26 | 成都中牧生物药业有限公司 | Composition for treating avian influenza |
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| 李继红: "《中药口服液防治鸡新城疫临床效果试验》", 《中兽医医药杂志》 * |
| 李继红等: "抗病毒口服液的质量标准研究", 《辽宁农业职业技术学院学报》 * |
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| CN103191263A (en) * | 2013-04-02 | 2013-07-10 | 河北农业大学 | Traditional Chinese medicine preparation for preventing and treating chicken kidney-type infectious bronchitis and preparation method thereof |
| CN103191240A (en) * | 2013-04-17 | 2013-07-10 | 山东省农业科学院家禽研究所 | Traditional Chinese veterinary medicine oral solution for preventing and treating viral diseases of poultry |
| CN103191240B (en) * | 2013-04-17 | 2014-10-29 | 山东省农业科学院家禽研究所 | Traditional Chinese veterinary medicine oral solution for preventing and treating viral diseases of poultry |
| CN104208168A (en) * | 2013-09-30 | 2014-12-17 | 郑州后羿制药有限公司 | Compound traditional Chinese medicine composition for treating poultry respiratory tract infection and preparation method thereof |
| CN104208168B (en) * | 2013-09-30 | 2016-08-17 | 郑州后羿制药有限公司 | A kind of for compound traditional Chinese medicine composite treating poultry respiratory infection and preparation method thereof |
| CN104922401A (en) * | 2015-05-22 | 2015-09-23 | 陶建壮 | Medicine for preventing and treating chicken pest |
| CN106038919A (en) * | 2016-07-14 | 2016-10-26 | 成都中牧生物药业有限公司 | Composition for treating avian influenza |
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