CN101797345B - Traditional Chinese medicine composition for preventing and treating viral diseases of poultry and preparation method - Google Patents
Traditional Chinese medicine composition for preventing and treating viral diseases of poultry and preparation method Download PDFInfo
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Abstract
The invention relates to a traditional Chinese medicine composition for preventing and treating the viral diseases of poultry and a preparation method. The traditional Chinese medicine composition is characterized by comprising the following raw materials in parts by weight: 5 to 22 parts of honeysuckle, 5 to 25 parts of radix isatidis, 4 to 18 parts of dandelion, 4 to 12 parts of coptis root, 4 to 15 parts of lophantherum gracile, 4 to 20 parts of green chiretta, 3 to 19 parts of figwort, 5 to 14 parts of rehmannia root, 3 to 15 parts of Chinese ephedra and 5 to 13 parts of licorice. Compared with the prior art, the traditional Chinese medicine composition has the advantages of naturalness, greenness and high efficiency, enhances the immunity of an organism, regulates the unbalance state of the organism by driving out evil spirits and strengthening the body resistance, promotes the growth and achieves the effect of treating both the principal and secondary aspects of a disease.
Description
Technical field
The present invention relates to veterinary drug technology for producing field, relate to a kind of efficient, safety, the prevention of low toxicity and Chinese medicine composition of treatment avian viral diseases and preparation method thereof specifically.
Background technology
Fast development and socioeconomic prosperity along with modern industry production, serious pollution and destruction are suffered in ecological environment, potent virus sexually transmitted disease such as infectious atypical pneumonia (SARS), high pathogenic avian influenza, porcine nameless high-fever, human influenza etc. are constantly provoked the health of humans and animals, and the existence of humans and animals is caused great threat.People press for a kind of efficient, low toxicity, prevention and the viral communicable newtype drug of treatment safely.
Chemotherapeutics is when killing pathogenic bacterium quickly and efficiently, not only animal is produced harm, the reduction fertility performance of drug-induced disease, importantly drug residue influences the safety and the human health of food, can cause that also pathogenic microorganism produces drug resistance, but the pathogenic microorganism of variation also contaminated environment cause the more propagation of serious disease; The vaccination of viral disease can produce resistance at specific pathogenic microorganism, but can not resist the virus of variation and other kinds, also has repeatedly immune operation to influence shortcomings such as fertility performance, increase production cost simultaneously.
Chinese medicine is the Chinese nation's " quintessence of Chinese culture ", and unique theory of medicine and Therapeutic Method are arranged, and has brought into play irreplaceable effect in the treatment of all previous epizootic disease viral disease.Chinese medicine reaches the effect for the treatment of both the principal and secondary aspects of a disease by the imbalance state of " get rid of evils and set upright " adjustment body; The reasonable compatibility of multi-flavor medicine in the prescription, at multiple paathogenic factor and approach, too many levels, the comprehensive resistance against diseases of resisting disease, improving body in multi-level, many target spots ground; According to the medication theory of plant-based medicine " integration of edible and medicinal herbs ", the advantage of green Chinese, natural, safe, low (or nothing) poison, noresidue seems more outstanding; Minister of Public Health Chen Zhu proposes to explore and to bring into play the advantage of Chinese medicine " preventive treatment of disease ", is the affirming of harm, purifying ecological environment of Chinese medicine being reduced infective virus; Chinese medicine also has multiple characteristic advantages such as expense is cheaper, clinical efficacy is definite, potential of innovation is huge, development space is wide.
Summary of the invention
Purpose of the present invention is given full play to the multiple characteristic advantage of Chinese medicine just, at a kind of Chinese medicine composition and preparation method of preventing and treating avian viral diseases that existing weak point provided in the above-mentioned prior art.Chinese medicine composition of the present invention has the advantage of natural green, efficient, human body immunity improving power.
Purpose of the present invention can realize by following technique measures:
The Chinese medicine composition of prevention of the present invention and treatment avian viral diseases comprises the raw material of following portions by weight ratio: wherein: 5～22 parts of Flos Loniceraes, 5～25 parts of Radix Isatidis, 4～18 parts of Herba Taraxacis, 4～12 parts of Rhizoma Coptidis, 4～15 parts of Herba Lophatheris, 4～20 parts of Herba Andrographis, 3～19 parts of Radix Scrophulariaes, 5～14 parts in the Radix Rehmanniae, 3～15 parts in Herba Ephedrae, 5～13 parts in Radix Glycyrrhizae.
The preparation method of Chinese medicine composition of the present invention comprises the steps:
A, carry out water extraction: 5～22 parts of extracting honeysuckles, 5～25 parts of Radix Isatidis, 4～18 parts of Herba Taraxacis, 4～12 parts of Rhizoma Coptidis, 4～15 parts of Herba Lophatheris, 4～20 parts of Herba Andrographis, 3～19 parts of Radix Scrophulariaes, 5～14 parts in the Radix Rehmanniae, 3～15 parts in Herba Ephedrae, 5～13 parts in Radix Glycyrrhizae by ratio of weight and the number of copies, water consumption is not got final product by water logging with raw medicinal material, put into water and soak 1-2h, and then extract 5-8h with hot reflux, filter, be concentrated into 1/100 one-tenth flowing soaking paste of former lixiviating solution afterwards;
B, the medicinal residues that water was carried carry out precipitate with ethanol 2 times, the ethanol content that at first adds 3-5 times of volume ratio in residue is that 70% ethanol carries out precipitate with ethanol 12-15h, and then the ethanol content that adds 3-5 times of volume ratio is that 80% ethanol carries out precipitate with ethanol 12-15h once more, cross the elimination residue, filtrate is carried out decompression recycling ethanol;
C, decompression recycling ethanol, concentrated extracting solution are to there not being the alcohol flavor; Add the resulting condensed water extract of a step, be diluted to recipe quantity 1g/mL with purified water afterwards; 4 ℃ of preservations are standby.
The principles of formulating prescriptions of the present invention are " eliminating pathogenic factor for supporting vital QI ".Collecting and summing up on the basis of numerous classical prescriptions, proved recipe, according to the prescription policy of " monarch, minister, help, make ", through testing and verification Cheng Fang in many ways.Extract the effective ingredient of different pharmaceutical through design, make it to bring into play synergism, wide spectrum, effect are more remarkable more to make the antiviral scope of medicine, simultaneously, promote body to produce powerful non-special and specific immune function, improve the ability that body is resisted various diseases.
Monarch drug is Flos Lonicerae, Radix Isatidis, Herba Taraxaci, Rhizoma Coptidis in the prescription, be the antipyretic and antidotal type medicine, have effects such as heat-clearing and toxic substances removing, heat radiation dispelling wind, diuresis promoting, dispersing swelling and dissipating binds, dampness pathogenic fire purging, the effect of directly virus killing of performance, sterilization, minimizing body inner virus, and the effective ingredient of Flos Lonicerae, Radix Isatidis, suppress the duplicating of virus, blocking virus propagation by improving content of medicines in the endochylema; Ministerial drug is Radix Scrophulariae, Herba Lophatheri, Herba Andrographis, have effects such as nourishing YIN to lower pathogenic fire, clearing away heat and promoting diuresis, reducing swelling and alleviating pain, can promote human body immune function to strengthen, produce a large amount of antiviral agents by specificity and non-specific mechanism, suppress virus protein and synthesize, influence the assembling and the release of virion, press down viricidal effect thereby reach; Adjuvant is the Radix Rehmanniae, Herba Ephedrae, Radix Glycyrrhizae, has Yin-nourishing and body fluid promoting, effects such as the cold expelling that induces sweat, depressed lung-energy dispersing, spleen invigorating and the property of medicine, can improve body's immunological function, promotes the body normal physiological function to coordinate, thereby reaches antiviral, constitutional curative effect.
Beneficial effect of the present invention is as follows:
Chinese medicine composition of the present invention has the advantage of natural green, efficient, human body immunity improving power compared to existing technology, by " get rid of evils and set upright " adjust body imbalance state, promote growth, reach the effect for the treatment of both the principal and secondary aspects of a disease.
Description of drawings
Fig. 1 immune organ index.
The contrast of Fig. 2 body weight gain.
The HA of NDV tires in Fig. 3 chick embryo allantoic liquid.
The specific embodiment
The present invention will be further described with the following Examples:
Embodiment 1:
A, 18 parts of extracting honeysuckles, 25 parts of Radix Isatidis, 12 parts of Herba Taraxacis, 4 parts of Rhizoma Coptidis, 4 parts of Herba Lophatheris, 20 parts of Herba Andrographis, 3 parts of Radix Scrophulariaes, 5 parts in the Radix Rehmanniae, 4 parts in Herba Ephedrae, Radix Glycyrrhizae carry out water extraction for 5 parts by ratio of weight and the number of copies, at first soak 1-2h, water consumption is not got final product by water logging with raw medicinal material, extract 5-8h with hot reflux then, filter, be concentrated into 1/100 one-tenth flowing soaking paste of former lixiviating solution afterwards;
B, the medicinal residues that water was carried carry out precipitate with ethanol 2 times, the ethanol content that at first adds 3-5 times of volume ratio in residue is that 70% ethanol carries out precipitate with ethanol 12-15h, and then the ethanol content that adds 3-5 times of volume ratio is that 80% ethanol carries out precipitate with ethanol 12-15h once more, cross the elimination residue, filtrate is carried out decompression recycling ethanol;
C, decompression recycling ethanol, concentrated extracting solution are to there not being the alcohol flavor; Add the resulting condensed water extract of a step, be diluted to recipe quantity 1g/mL with purified water afterwards; 4 ℃ of preservations are standby.
Embodiment 2:
A, 5 parts of extracting honeysuckles, 18 parts of Radix Isatidis, 18 parts of Herba Taraxacis, 5 parts of Rhizoma Coptidis, 15 parts of Herba Lophatheris, 4 parts of Herba Andrographis, 5 parts of Radix Scrophulariaes, 10 parts in the Radix Rehmanniae, 15 parts in Herba Ephedrae, Radix Glycyrrhizae carry out water extraction for 5 parts by ratio of weight and the number of copies, at first soak 1-2h, water consumption is not got final product by water logging with raw medicinal material, extract 5-8h with hot reflux then, filter, be concentrated into 1/100 one-tenth flowing soaking paste of former lixiviating solution afterwards;
B, the medicinal residues that water was carried carry out precipitate with ethanol 2 times, the ethanol content that at first adds 3-5 times of volume ratio in residue is that 70% ethanol carries out precipitate with ethanol 12-15h, and then the ethanol content that adds 3-5 times of volume ratio is that 80% ethanol carries out precipitate with ethanol 12-15h once more, cross the elimination residue, filtrate is carried out decompression recycling ethanol;
C, decompression recycling ethanol, concentrated extracting solution are to there not being the alcohol flavor; Add the resulting condensed water extract of a step, be diluted to recipe quantity 1g/mL with purified water afterwards; 4 ℃ of preservations are standby.
Embodiment 3:
A, 22 parts of extracting honeysuckles, 5 parts of Radix Isatidis, 4 parts of Herba Taraxacis, 12 parts of Rhizoma Coptidis, 4 parts of Herba Lophatheris, 4 parts of Herba Andrographis, 19 parts of Radix Scrophulariaes, 14 parts in the Radix Rehmanniae, 3 parts in Herba Ephedrae, Radix Glycyrrhizae carry out water extraction for 13 parts by ratio of weight and the number of copies, at first soak 1-2h, water consumption is not got final product by water logging with raw medicinal material, extract 5-8h with hot reflux then, filter, be concentrated into 1/100 one-tenth flowing soaking paste of former lixiviating solution afterwards;
B, the medicinal residues that water was carried carry out precipitate with ethanol 2 times, the ethanol content that at first adds 3-5 times of volume ratio in residue is that 70% ethanol carries out precipitate with ethanol 12-15h, and then the ethanol content that adds 3-5 times of volume ratio is that 80% ethanol carries out precipitate with ethanol 12-15h once more, cross the elimination residue, filtrate is carried out decompression recycling ethanol;
C, decompression recycling ethanol, concentrated extracting solution are to there not being the alcohol flavor; Add the resulting condensed water extract of a step, be diluted to recipe quantity 1g/mL with purified water afterwards; 4 ℃ of preservations are standby.
Test effect of the present invention is as follows:
(1) toxicity test of medicine
Test method: the compositions of 3 groups of embodiment is carried out 0,5,10,50,100 times of dilution respectively with normal saline, inject the SPF chick embryo allantois intracavity of 9 ages in days respectively, 0.2mL/ only, annotate again once every 12h, inject altogether 2 times, each handles 4 parallel laboratory tests, establishes the normal saline matched group, shared 72 pieces of Embryo Gallus domesticus.After the injection, hatch 72h in 37 ℃ of constant incubators, observe Embryo Gallus domesticus and survive state every day, and the not dead Embryo Gallus domesticus of 72h continues hatching until hatching.Dead minimum concentration occurring with Embryo Gallus domesticus is the minimum lethal concentration of this medicinal liquid.
Result of the test: all none death of concentration liquid inoculated into chick embryo, the chickling of hatching is normal.Therefore, medicinal liquid to the minimum lethal dose of Embryo Gallus domesticus greater than 1g/mL.
Conclusion: the Embryo Gallus domesticus toxicity of 3 groups of embodiment is minimum or nontoxic.
(2) compositions enhancing immunity, growth promoting function
Test method: buy 480 of the assorted chickens of 1 age in days meat and be laboratory animal, the normal raising was divided into 4 groups at random to the 9th day: example one administration group, example two administration groups, example three administration groups, blank group, and each is handled and divides 6 repetitions, and each repeats 20 chickens.Began experiment on the 10th day, adopt the mode of oral medicine feed, respectively feed once sooner or later, interval 12h, 0.2mL/ time; Matched group gives normal saline and substitutes medicinal liquid.Normal feeding manner is fed, and does not carry out the immunity and the treatment of any eqpidemic disease.16 days experimental periods.
The 16th day (i.e. 25 ages in days) after the medication, each test group chicken blood-letting causes death, claim its body weight, get thymus, spleen and fabricius bursa, unnecessary tissues such as fatty tissue around peeling off and sarolemma, with weighing after filter paper suction part, calculate each immune organ by following formula: the organ index of fabricius bursa, spleen and thymus, as the index that promotes immunocompetence.
Heavy (the mg)/body weight (10g) of fabricius bursa index (mg/g)=fabricius bursa
Heavy (the mg)/body weight (10g) of thymus index (mg/g)=thymus
Heavy (the mg)/body weight (10g) of spleen index (mg/g)=spleen
Duration of test pursues the record of only weighing at the 16th age in days and the 25th age in days respectively, as the index of medicinal liquid to the energy for growth influence.
Result of the test:
A immune organ index detects (referring to Fig. 1)
From the immune organ index as can be seen, fabricius bursa index, thymus index and the index and spleen index of 3 groups of embodiment schemes is all apparently higher than the blank group, illustrate that 3 groups of embodiment schemes all can promote the growth of immune organ, effectively improve the immunocompetence and the resistance against diseases of body, wherein the fabricius bursa index of embodiment one is higher, and thymus index and the index and spleen index of embodiment two are higher.
The mensuration of B body weight (referring to Fig. 2);
By body weight determination, one week of different experiments group back body weight demonstration significant difference, 3 embodiment group chicken average weights are than the high 70-90g of matched group; During off-test, 3 embodiment group average weights are higher than matched group 110-135g.
Conclusion: three embodiment schemes all have certain facilitation to growth and immunity.
(3) Embryo Gallus domesticus of anti-newcastle disease virus test
Test method: compositions and virus are acted in different ways.Be " prevention " mode with the virus of reinjecting behind the adding compositions 2-4h; Be " directly virus killing " mode with compositions and the viral Embryo Gallus domesticus that behind interaction in vitro 2-4h, reinjects; To add compositions behind the viral 2-4h of elder generation's injection is " treatment " mode.Simultaneously, virus and normal saline are injected Embryo Gallus domesticus be made as the positive and negative control, as measurement index, calculation composition is to the protective rate of Embryo Gallus domesticus with the survival rate of the variation of Embryo Gallus domesticus virus titer and Embryo Gallus domesticus:
Protective rate=(total Embryo Gallus domesticus number-dead Embryo Gallus domesticus number)/total Embryo Gallus domesticus number * 100%.
The concentration of injecting compositions is 1g/mL, is experiment material with the SPF Embryo Gallus domesticus of 9-11 age in days, and virus is selected newcastle disease mesogenic live vaccine (I system) for use, the equal dosage of injection volume is 0.2mL/ piece, 3 repetitions are established in every kind of processing, and each repeats 10 pieces of Embryo Gallus domesticus, shared 330 pieces of SPF Embryo Gallus domesticus.37 ℃ of calorstats are cultivated.Discard 24h with interior dead Embryo Gallus domesticus, collect the allantoic fluid of 24-72h survival Embryo Gallus domesticus, adopt the titre of blood clotting measuring allantoic fluid virus.
Result of the test:
The A HA situation (referring to Fig. 3) of tiring;
For the ease of contrasting with the experimental group virus titer, the virus of positive control is on average tired to be highlighted by three kinds of mode lines.As can be seen from the figure: three kinds of model of action of 3 embodiment can both press down the growth of Avian pneumo-encephalitis virus in Embryo Gallus domesticus extremely to some extent, wherein the model of action of " prevention " is the most obvious to the inhibitory or killing effect of virus, the mode of " prevention " can press down effectively kills (promptly reducing) viral 3.7-4.5 titre, makes virus survival total amount reduce 49-60%; " directly " be 2.5-4.5 titre but model of action can kill the virus effectively, and virus survival total amount reduces 33-60%; " treatment " be 1.8-2.3 titre but model of action can kill the virus effectively, and virus survival total amount reduces 24-31%.The virus titer of negative normal saline contrast is zero.
The protective rate of B Embryo Gallus domesticus
Medicinal liquid is to the protective rate (%) of Embryo Gallus domesticus
Test group and matched group all do not have chicken embryo death in the 24h.In the 60h, 3 embodiment groups do not have chicken embryo death, and protective rate is 100%, the dead 12 pieces of Embryo Gallus domesticus of virus control group (totally 30 pieces), and mortality rate is 40%, protective rate is 60%; During 86h, embodiment begins to occur 1-3 piece of chicken embryo death, and each organizes protective rate appearance decline in various degree, and the chicken embryo death rate of virus control group is up to 80% at this moment, and protective rate also drops to 20%.The embodiment test group is than the protective rate of positive control increase 70-80%, and wherein embodiment one and example two increase the protective rate of 73.3-80%, and embodiment three increases the protective rate of 70-73.3%.
(4) clinical trial of control bursa of fabricius diseases
Test method:
1. experimental animal grouping
The SPF chicken of adopting 30 ages in days is an experimental animal, and the test grouping sees the following form.
2. prevention+treatment group (being SM): every day, medicine feed was 2 times, interval 12h, each 0.2mL/ is only inferior, behind the medicine feed 3d, infect bursal disease virus and make pathological model (IBDV-BC6/85 virulent strain diluent eye dripping 0.05mL/ is only), the clinical symptoms that infects back 2d observation experiment chicken, simultaneously, lasting medicine feed is treated, Therapy lasted 10d.
3. treatment (i.e. infection back medication): infect bursal disease virus making pathological model (the same), behind the infective virus 4h, the beginning medicine feed, every day 2 times, interval 12h, each 0.2mL/ is only inferior, and the clinical symptoms of observation experiment chicken continues medicine feed 10d and treats.
4. matched group (promptly infect after not medication): according to the counteracting toxic substances dosage (the same) of pathological model chicken is carried out counteracting toxic substances and infect, do not carry out any prevention and treatment.
5. the assay method of observation index: 3d behind the counteracting toxic substances, each experimental group appoint and get 5 chickens and cut open and kill, and gather fabricius bursa and observe pathological change, calculate the pathological changes incidence rate; 10d cuts open all experimental chickens and kills behind the counteracting toxic substances, observes the pathological change of statistics fabricius bursa.
Result of the test:
The clinical manifestation of test chicken behind the counteracting toxic substances 3d
Behind the counteracting toxic substances 3d, the chicken clinical manifestation of prevention+treatment group is normal substantially; The indivedual chickens of treatment group occur slight spiritual depressed, accidental loose stool; The chicken performance spirit of virus control group is depressed, appetite detracts, feather is fluffy, symptoms such as upright and white loose stool are stayed in motion.
The 3d fabricius bursa is observed pathological change behind the counteracting toxic substances
Behind the counteracting toxic substances 3d, the fabricius bursa of 3 groups of embodiment chickens all has enlargement slightly; Wherein gel-shaped exudate and odd inner petechia appear in the fabricius bursa outside of the prevention of embodiment two+treatment group and treatment group; The prevention of embodiment one and example two+treatment group and treatment group only have inner gel-shaped exudate slightly; The fabricius bursa pathological changes incidence rate of 3 groups of embodiment is 1/5-3/5.Virus control group fabricius bursa is seen enlargement, flavescence and inner fragmentary petechia, and wherein, outside and inner gel-shaped exudate is obvious, and the pathological changes incidence rate reaches 5/5.
Fabricius bursa pathology changes behind the counteracting toxic substances 10d
| Flavescence | Outside gel-shaped exudate | Inner gel-shaped exudate | Inner petechia | |
| Embodiment one prevention+treatment group | + | - | - | - |
| Embodiment two prevention+treatment groups | + | - | + | - |
| Embodiment three prevention+treatment groups | + | - | - | - |
| Embodiment one treatment group | + | - | + | - |
| Embodiment two treatment groups | + | - | + | - |
| Embodiment three treatment groups | + | - | - | - |
| The virus control group | ++ | +++ | +++ | ++ |
Behind the counteracting toxic substances 10d, the slight flavescence of the fabricius bursa of 3 groups of embodiment, wherein there is a spot of gel-shaped exudate the prevention of the treatment group of embodiment one, embodiment two+treatment group and treatment group fabricius bursa inside, and the bleeding of fabricius bursa, outside gel-shaped exudate disappear, and inner gel-shaped oozes out minimizing; The enlargement of virus control group fabricius bursa, flavescence overweight the embodiment group, and there is the area-shaped petechia inside, and inside and outside gel-shaped exudate is serious.
Conclusion: by the treatment of each group example composition, the fabricius bursa symptom takes an evident turn for the better, and Chinese medicine composition has certain preventive and therapeutic effect to bursa of fabricius diseases.
5 Chinese medicine composition clinical efficacies
Example one: large quantities of death appear in chicken farm, Henan 38 age in days broiler chicken, full group is more than totally 5800 chicken, nearly 1000 chickens morbidity is arranged, 139 chickens that die in heaven, most of sick chicken shows lethargy, feed intake decline, cough sneeze, mouth breathing, draws symptoms such as white loose stool, feather is loose disorderly, double-vane is sagging, and suspection chicken biography is propped up and bacterial disease mixed infection.Using the Chinese medicine composition of the embodiment of the invention one preparation treats, preceding 3 days is that drinking-water carries out full group's drinking-water with the medicinal liquid, the sick chicken that can not drink water isolates to be fed, every day 4 times, each 0.5mL, the chicken of serious symptom is eliminated buried, before and after the treatment colony house and surrounding thereof is carried out the strict sterilization processing.Medication is increase morbidity chicken number, the clinical symptoms of sick chicken and mental status improvement again of full group after 3 days.After the medication 7 days, 776 sick chicken clinical symptom disappearance are got well substantially, and cure rate reaches 90%.Cure after one month and pay a return visit, not recurrence again.
Example two: the state of an illness appears in the individual egg chick of raising 3500 28 ages in days at family in Qingdao, about 850 sick chickens performance Caput Gallus domesticuss hang down ground, drowsiness, appetite is useless exhausted, sick chicken anus pecking occurs, discharges white thickness water sample loose stool, is diagnosed as strong pathogenic fabricius bursa through veterinary inspection and infects.Use the Chinese medicine composition of the embodiment of the invention three preparations to treat, carried out full group's drinking-water medicine feed in preceding 3 days, the sick chicken that can not drink water isolates the injection high immunity yolk antibody, and the weak chicken of serious symptom is in time eliminated and burns.Before and after the treatment colony house and surrounding thereof are carried out the strict sterilization processing.After the medication 3 days, except that 33 of the serious sick chicken deaths of morbidity, Quan Qunwei increases morbidity chicken number again, and the clinical symptoms and the mental status of sick chicken take a turn for the better to some extent.After the medication 7 days, 809 sick chicken clinical symptom disappearance are got well substantially, and 8 small and weak chickens are eliminated.Cure and pay a return visit not morbidity again after one month.
Example three: atypical newcastle takes place in certain body raiser laying hen of Hebei, one 3000, obvious egg drop reduction appears in the laying hen of 250 ages in days, is that atypical newcastle infects after diagnosing.Use the Chinese medicine composition of the embodiment of the invention two preparations and treat, adopt the mode of drinking-water administration to concentrate administration, before and after the treatment hen house and surrounding are carried out strict sterilization.Chicken is mined massively after 3 days that appetite rises, egg drop reduction is slowed down in medication, and medication is after 6 days, and laying eggs begins to recover, and it is normal that the shell egg recovers, and laying eggs through medication in 10 days returns to formerly 93%, and cure rate reaches 100%.
6 advantages
Compositions of the present invention is primarily aimed at the poultry virus disease certain curative effect, and removing due to illness rapidly by number of ways, poison infects caused heating, body injury, alleviate clinical disease, promote sick fowl to produce immunity, recover to search for food, promote laying rate and egg product matter to recover.In clinical control experiment; by improving direct kill virus and human body immunity improving performance; reduced the M ﹠ M of animal; improved protective rate, promoted animal growth, the 3rd day control morbidity scale no longer enlarges after the general medication; clinical symptoms begins to alleviate; clinical symptom disappearance after 5～7 days is almost recovered, and has good production practical function really.This product is drug residue free, low toxicity or nontoxic in use, and is little to the body zest, do not influence and lays eggs, be difficult for producing drug resistance, and be the fully natural green safe drugs of trusting fully.
Claims (2)
1. the Chinese medicine composition of prevention and treatment avian viral diseases is characterized in that: the raw material weight umber of this Chinese medicine composition is such as down: wherein: 5～22 parts of Flos Loniceraes, 5～25 parts of Radix Isatidis, 4～18 parts of Herba Taraxacis, 4～12 parts of Rhizoma Coptidis, 4～15 parts of Herba Lophatheris, 4～20 parts of Herba Andrographis, 3～19 parts of Radix Scrophulariaes, 5～14 parts in the Radix Rehmanniae, 3～15 parts in Herba Ephedrae, 5～13 parts in Radix Glycyrrhizae.
2. preparation method that is used for the described Chinese medicine composition of claim 1: it is characterized in that: comprise the steps:
A, carry out water extraction: 5～22 parts of extracting honeysuckles, 5～25 parts of Radix Isatidis, 4～18 parts of Herba Taraxacis, 4～12 parts of Rhizoma Coptidis, 4～15 parts of Herba Lophatheris, 4～20 parts of Herba Andrographis, 3～19 parts of Radix Scrophulariaes, 5～14 parts in the Radix Rehmanniae, 3～15 parts in Herba Ephedrae, 5～13 parts in Radix Glycyrrhizae by ratio of weight and the number of copies, water consumption is not got final product by water logging with raw medicinal material, put into water and soak 1-2h, and then extract 5-8h with hot reflux, filter, be concentrated into 1/100 one-tenth flowing soaking paste of former lixiviating solution afterwards;
B, the medicinal residues that water was carried carry out precipitate with ethanol 2 times, the ethanol content that at first adds 3-5 times of volume ratio in residue is that 70% ethanol carries out precipitate with ethanol 12-15h, and then the ethanol content that adds 3-5 times of volume ratio is that 80% ethanol carries out precipitate with ethanol 12-15h once more, cross the elimination residue, filtrate is carried out decompression recycling ethanol;
C, decompression recycling ethanol, concentrated extracting solution are to there not being the alcohol flavor; Add the resulting condensed water extract of a step, be diluted to recipe quantity 1g/mL with purified water afterwards, 4 ℃ of preservations are standby.
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| CN1899498A (en) * | 2006-07-05 | 2007-01-24 | 河北农业大学 | Medicine for preventing and controlling chicken infectious fabricius bursa disease |
| CN101543602A (en) * | 2008-03-28 | 2009-09-30 | 天科仁祥技术（北京）有限责任公司 | Chinese medicinal composition for treating upper respiratory tract infection and preparation method thereof |
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| CN101543602A (en) * | 2008-03-28 | 2009-09-30 | 天科仁祥技术（北京）有限责任公司 | Chinese medicinal composition for treating upper respiratory tract infection and preparation method thereof |
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