CN105641031A - Compound pharmaceutical composition for strengthening body resistance and removing toxin, use and feed thereof - Google Patents
Compound pharmaceutical composition for strengthening body resistance and removing toxin, use and feed thereof Download PDFInfo
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Abstract
The invention discloses a compound pharmaceutical composition for strengthening body resistance and removing toxin, use and feed thereof. The compound pharmaceutical composition is a traditional Chinese medicine extract formed by water decoction and extraction processing of astragalus, Chinese angelica, echinacea, Poria cocos, bighead atractylodes rhizome, Radix Isatidis, Folium Isatidis, Herba Epimedii and licorice, can be added into feed directly or further processed into granules, tablets, oral liquid and injection, etc. The compound traditional Chinese medicine pharmaceutical composition is compounded according to the Chinese veterinary drug prescription according to the principle of differentiated treatment of symptoms, repeated clinical tests prove that the compound pharmaceutical composition has the efficacy of strengthening vital qi, consolidating the basis, and removing pathogenic evils, can strengthen the body immunity of cultured animals and improve the disease resistance of cultured animals. The compound traditional Chinese medicine pharmaceutical composition provided by the invention has the advantages of simple production process, convenient use, significant effect, no residue, green and environmental protection, is conducive to mass prevention and mass treatment of cultured animals, and is beneficial to prevention and control of animal epidemic diseases.
Description
Technical field
The present invention relates to a kind of Fuzheng Jiedu compound medicament composition, its purposes and feedstuff, this medicine has Fuzheng Jiedu function, can strengthen cultivated animals immunity of organisms, improves cultivated animals premunition, belongs to field of veterinary.
Background technology
Along with development intensive, large-scale cultivation, cultivated animals disease is wreaked havoc, and causes serious economic loss and social danger. According to China Animal Agriculture Association initial estimate, within 2013, bird flu causes broiler baby chick direct losses more than 3,700,000,000 yuan, and live chickens and chicken meat product lost on sale are more than 13,000,000,000 yuan. The foot and mouth disease epidemic situation that Korea S will break out the end of the year in 2010 diffuses to 149 farms of 10, whole nation city, road, prefecture, 70 cities, adds up to slaughter the domestic animal such as pig, cattle more than 330 ten thousand, and domestic animal landfill place is located up to more than 4600, direct economic loss about 1,800,000,000 dollars. Japan will break out bird flu epidemic situation the end of the year in 2010, and about more than 100 ten thousand poultry are slaughtered. Currently, animal epidemics is directly connected to the success or failure of cultivation, controls animal epidemics, and development healthy aquaculture becomes the important topic of aquaculture.
At present, although vaccine obtains the attention of government, enterprise and raiser as prevention and control infectious disease means, in prevention and control epidemic disease, it is made that indelible contribution, but the defect of its existence is also well-known. Such as, Attenuate vaccine returns by force, causes illness outbreak; Cause of disease makes a variation, and causes the inefficacy of original vaccine, it is impossible to effectively control epidemic disease; Immune antibody level is uneven, there is the hidden danger of outburst epidemic disease; When bacterium occurs many types of, only leaning on unitary type vaccine is uncontrollable firmly epidemic disease. And it is big to vaccinate not only labor intensity, labor intensive, return animal cause stress, affect it and produce performance. Currently, market in urgent need effectively prevents and treats means and the related drugs of cultivated animals disease disease.
Traditional Chinese veterinary medicine thinks that carcass and natural environment are closely related, and " YIN and YANG in a relative equilibrium, life activities being normally maintained " focuses on Yin Yang balancing; " healthy energy internal memory, heresy can not be done ", focuses on the non-specific immunity improving body. Regulate animal body by herbal medicine, strengthen immunocompetence, be greatly improved the quality of cultivated animals and overall epidemic prevention level, reduce sickness rate. Additionally, herbal medicine does not have or seldom has side effect, the problems such as antibiotic usage, drug residue and Drug abuse can be reduced, meet the requirement of green, safety, Organic food. Herbal mixture pharmaceutical composition of the present invention controls principle prescription according to herbal medicine syndrome differentiation of formulas opinion, has reinforcing body resistance, and it is basic to consolidate, and the prevention and control of current animal epidemic are had great significance by effect of dispeling disease and evil.
Summary of the invention
It is an object of the invention to provide a kind of efficient, natural, safe herbal mixture pharmaceutical composition.
Another object of the present invention is to provide the preparation method and its usage of this herbal mixture pharmaceutical composition.
It is an object of the invention to be achieved through the following technical solutions:
The present invention provides the herbal mixture pharmaceutical composition of a kind of Fuzheng Jiedu, and it contains active component, and described active component is made up of the Radix Astragali, Radix Angelicae Sinensis, Echinacea, Poria, the Rhizoma Atractylodis Macrocephalae, Radix Isatidis, Folium Isatidis, Herba Epimedii, Radix Glycyrrhizae.
Wherein, described active component by weight, is made up of Radix Astragali 1-50 part, Radix Angelicae Sinensis 1-20 part, Echinacea 1-80 part, Poria 1-20 part, Rhizoma Atractylodis Macrocephalae 1-20 part, Radix Isatidis 1-40 part, Folium Isatidis 1-40 part, Herba Epimedii 1-30 part, Radix Glycyrrhizae 1-20 part.
Further, described active component by weight, is made up of Radix Astragali 5-40 part, Radix Angelicae Sinensis 2-15 part, Echinacea 10-70 part, Poria 2-15 part, Rhizoma Atractylodis Macrocephalae 2-15 part, Radix Isatidis 5-30 part, Folium Isatidis 5-30 part, Herba Epimedii 3-20 part, Radix Glycyrrhizae 2-15 part.
Further, described active component by weight, is made up of Radix Astragali 10-30 part, Radix Angelicae Sinensis 3-10 part, Echinacea 30-60 part, Poria 3-10 part, Rhizoma Atractylodis Macrocephalae 3-10 part, Radix Isatidis 10-20 part, Folium Isatidis 10-20 part, Herba Epimedii 5-15 part, Radix Glycyrrhizae 3-10 part.
The preparation method that present invention also offers herbal mixture pharmaceutical composition, comprises the steps:
Weigh each medical material by weight, pulverize, add 8-10 times amount soak by water 1-3 time, each 1-2h, merge decoction liquor, filter, filtrate is concentrated in right amount, adds 1-3 times of dehydrated alcohol and staticly settles overnight, filters, filtrate is concentrated into extractum shape, and corresponding dosage form made by the corresponding pharmaceutically acceptable carrier of dried addition or adjuvant.
The herbal mixture pharmaceutical composition of the present invention, it can be used for preparing the medicine with Fuzheng Jiedu function, enhancing cultivated animals immunity of organisms and raising cultivated animals resistance against diseases aspect.
The herbal mixture pharmaceutical composition of the present invention can be directly appended in feedstuff, and therefore, the present invention also provides for the feedstuff containing described herbal mixture pharmaceutical composition.
The herbal mixture pharmaceutical composition of the present invention is made up of the Radix Astragali, Radix Angelicae Sinensis, Echinacea, Poria, the Rhizoma Atractylodis Macrocephalae, Radix Isatidis, Folium Isatidis, Herba Epimedii, Radix Glycyrrhizae, Radix Astragali benefiting QI for strengthening the superficies, righting gas of rectifying in side, and Echinacea promotes Immune Function In Animals, and two medicines are principal agent altogether; Chinese angelica blood supplementing is invigorated blood circulation, Rhizoma Atractylodis Macrocephalae invigorating the spleen and replenishing QI, Poria spleen invigorating mind calming, helps principal agent strengthening the body resistance, is for ministerial drug; Radix Isatidis, Folium Isatidis heat-clearing and toxic substances removing, removing heat from blood sore-throat relieving, Herba Epimedii kidney-replenishing, wind-damp dispelling, is for adjuvant drug; Radix Glycyrrhizae invigorating the spleen and replenishing QI, and middle emergency, coordinating the actions of various ingredients in a prescription, be for making medicine. All medicines share, and play strengthening the body resistance, the merit of eliminating evil removing toxic substances altogether.
The herbal mixture pharmaceutical composition of the present invention is processed through decocting extraction by the Radix Astragali, Radix Angelicae Sinensis, Echinacea, Poria, the Rhizoma Atractylodis Macrocephalae, Radix Isatidis, Folium Isatidis, Herba Epimedii, Radix Glycyrrhizae, can be directly appended in feedstuff or be processed further making granule, tablet, oral liquid and injection etc., there is Fuzheng Jiedu function, cultivated animals immunity of organisms can be strengthened, improve cultivated animals resistance against diseases. This herbal mixture pharmaceutical composition is easy to use, effective, noresidue, environmental protection.
The advantage of herbal mixture pharmaceutical composition of the present invention and having the beneficial effects that:
1) herbal mixture pharmaceutical composition of the present invention controls principle prescription according to herbal medicine syndrome differentiation of formulas opinion, has reinforcing body resistance, consolidates effect basic, dispeling disease and evil;
2) herbal mixture pharmaceutical composition of the present invention is through many-sided clinical trial checking, can be obviously enhanced cultivated animals immunity, improve cultivated animals premunition;
3) herbal mixture pharmaceutical composition of the present invention is pure Chinese medicinal preparation, and noresidue, toxic and side effects are little, do not produce drug resistance, for green herbal medicine;
4) herbal mixture pharmaceutical composition Chinese crude drug of the present invention is natural Chinese medicines material, wide material sources, has recyclability, does not produce the problem of environmental pollutions such as synthetic pharmaceutical drugs, meets environment-friendly type, Sustainable type social development requirement;
5) herbal mixture pharmaceutical composition production technology of the present invention is simple, easy to use, is beneficial to the mass prevention and mass treatment of cultivated animals.
Detailed description of the invention
Following example are used for illustrating the present invention, but are not limited to the scope of the present invention.
Embodiment 1
(1) raw material containing following weight is weighed:
The Radix Astragali 1 part, Radix Angelicae Sinensis 1 part, Echinacea 80 parts, 1 part of Poria, the Rhizoma Atractylodis Macrocephalae 1 part, Radix Isatidis 40 parts, Folium Isatidis 40 parts, Herba Epimedii 30 parts, 20 parts of Radix Glycyrrhizae.
(2) weigh each medical material by weight ratio, pulverize, add 10 times amount soak by water 1 time, each 2h, merge decoction liquor, filter, filtrate is concentrated in right amount, adds 2 times of dehydrated alcohol and staticly settles overnight, filters, and filtrate is concentrated into extractum shape, is drying to obtain.
Embodiment 2
(1) raw material containing following weight is weighed:
The Radix Astragali 50 parts, Radix Angelicae Sinensis 20 parts, Echinacea 1 part, 20 parts of Poria, the Rhizoma Atractylodis Macrocephalae 20 parts, Radix Isatidis 1 part, Folium Isatidis 1 part, Herba Epimedii 1 part, 1 part of Radix Glycyrrhizae.
(2) weigh each medical material by weight ratio, pulverize, add 8 times amount soak by water 2 times, each 1.5h, merge decoction liquor, filter, filtrate is concentrated in right amount, adds 1 times of dehydrated alcohol and staticly settles overnight, filters, and filtrate is concentrated into extractum shape, is drying to obtain.
Embodiment 3
(1) raw material containing following weight is weighed:
The Radix Astragali 5 parts, Radix Angelicae Sinensis 2 parts, Echinacea 70 parts, 15 parts of Poria, the Rhizoma Atractylodis Macrocephalae 15 parts, Radix Isatidis 5 parts, Folium Isatidis 5 parts, Herba Epimedii 3 parts, 2 parts of Radix Glycyrrhizae.
(2) weigh each medical material by weight ratio, pulverize, add 12 times amount soak by water 2 times, each 1h, merge decoction liquor, filter, filtrate is concentrated in right amount, adds 3 times of dehydrated alcohol and staticly settles overnight, filters, and filtrate is concentrated into extractum shape, is drying to obtain.
Embodiment 4
(1) raw material containing following weight is weighed:
The Radix Astragali 40 parts, Radix Angelicae Sinensis 15 parts, Echinacea 10 parts, 2 parts of Poria, the Rhizoma Atractylodis Macrocephalae 2 parts, Radix Isatidis 30 parts, Folium Isatidis 30 parts, Herba Epimedii 20 parts, 15 parts of Radix Glycyrrhizae.
(2) each medical material is weighed by weight ratio, pulverize, add 10 times amount soak by water 1 time, each 2h, merge decoction liquor, filtering, filtrate is concentrated in right amount, adds 2 times of dehydrated alcohol and staticly settles overnight, filter, filtrate is concentrated into extractum shape, adds appropriate soluble starch mix homogeneously, dries and makes granule.
Embodiment 5
(1) raw material containing following weight is weighed:
The Radix Astragali 10 parts, Radix Angelicae Sinensis 3 parts, Echinacea 60 parts, 10 parts of Poria, the Rhizoma Atractylodis Macrocephalae 10 parts, Radix Isatidis 10 parts, Folium Isatidis 10 parts, Herba Epimedii 5 parts, 3 parts of Radix Glycyrrhizae.
(2) each medical material is weighed by weight ratio, pulverize, add 12 times amount soak by water 2 times, each 1.5h, merge decoction liquor, filtering, filtrate is concentrated in right amount, adds 1 times of dehydrated alcohol and staticly settles overnight, filter, filtrate is concentrated into extractum shape, adds appropriate soluble starch mix homogeneously, dries and makes granule.
Embodiment 6
Weigh the raw material containing following weight:
The Radix Astragali 30 parts, Radix Angelicae Sinensis 10 parts, Echinacea 30 parts, 3 parts of Poria, the Rhizoma Atractylodis Macrocephalae 3 parts, Radix Isatidis 20 parts, Folium Isatidis 20 parts, Herba Epimedii 15 parts, 10 parts of Radix Glycyrrhizae.
(2) weigh each medical material by weight ratio, pulverize, add 8 times amount soak by water 3 times, each 1.5h, merge decoction liquor, filter, filtrate is concentrated in right amount, adds 2 times of dehydrated alcohol and staticly settles overnight, filters, and filtrate is concentrated into extractum shape, is drying to obtain.
Embodiment 7
(1) raw material containing following weight is weighed:
The Radix Astragali 25 parts, Radix Angelicae Sinensis 5 parts, Echinacea 50 parts, 5 parts of Poria, the Rhizoma Atractylodis Macrocephalae 5 parts, Radix Isatidis 15 parts, Folium Isatidis 15 parts, Herba Epimedii 10 parts, 5 parts of Radix Glycyrrhizae.
(2) weigh each medical material by weight ratio, pulverize, add 10 times amount soak by water 3 times, each 1.5h, merge decoction liquor, filter, filtrate is concentrated in right amount, adds 2 times of dehydrated alcohol and staticly settles overnight, filters, and filtrate is concentrated into extractum shape, is drying to obtain.
Embodiment 8
(1) raw material containing following weight is weighed:
The Radix Astragali 25 parts, Radix Angelicae Sinensis 5 parts, Echinacea 75 parts, 20 parts of Poria, the Rhizoma Atractylodis Macrocephalae 20 parts, Radix Isatidis 25 parts, Folium Isatidis 10 parts, Herba Epimedii 10 parts, 5 parts of Radix Glycyrrhizae.
(2) weigh each medical material by weight ratio, pulverize, add 8 times amount soak by water 3 times, each 1.5h, merge decoction liquor, filter, filtrate is concentrated in right amount, adds 2 times of dehydrated alcohol and staticly settles overnight, filters, and filtrate is concentrated into extractum shape, is drying to obtain.
Embodiment 9
(1) raw material containing following weight is weighed:
The Radix Astragali 40 parts, Radix Angelicae Sinensis 10 parts, Echinacea 40 parts, 10 parts of Poria, the Rhizoma Atractylodis Macrocephalae 10 parts, Radix Isatidis 40 parts, Folium Isatidis 20 parts, Herba Epimedii 20 parts, 10 parts of Radix Glycyrrhizae.
(2) weigh each medical material by weight ratio, pulverize, add 10 times amount soak by water 2 times, each 1.5h, merge decoction liquor, filter, filtrate is concentrated in right amount, adds 2 times of dehydrated alcohol and staticly settles overnight, filters, and filtrate is concentrated into extractum shape, is drying to obtain.
Prophylactic treatment effect infectious coryza of chicken is sick for verifying herbal mixture pharmaceutical composition of the present invention, pharmaceutical composition of the present invention is carried out clinical observation on the therapeutic effect test, the beneficial effect of of the present invention herbal mixture drug pharmaceutical compositions is expanded on further below by way of test example.
The impact of mice carbonic clearance ability is tested by test example 1 embodiment of the present invention
By observing the herbal mixture pharmaceutical composition of the present invention impact on mice carbonic clearance ability, investigate the immunoenhancement result of herbal mixture pharmaceutical composition of the present invention.
1 material
Trial drug: embodiment of the present invention 1-5 herbal mixture pharmaceutical composition; Control drug: astragalus polysaccharide extract.
Experimental animal: BALB/C mice, male, 70, purchased from Beijing Vital River Experimental Animals Technology Co., Ltd..
2 methods
Test sets 7 groups altogether, often 10 mices of group. Gastric infusion, successive administration 7 days, packet situation and administrations are specifically shown in table 1. During test, free choice feeding drinking-water, tests the 8th day, and often group mice is weighed, and injects the india ink of dilution from mouse tail vein, calculates by every 10g body weight 0.1ml. Treat that prepared Chinese ink injects, immediately timing. Inject after prepared Chinese ink 2,10 minutes, take blood 30 �� l from angular vein clump respectively, exist side by side and be namely added into 3ml0.1%Na2CO3In solution. With 751 type spectrophotometers at 600nm wavelength place densitometric value (OD), with 0.1%Na2CO3Solution makes blank.
After off-test, by sacrifice, take liver and spleen, blot organ surface blood stains with filter paper, weigh. The ability of mice carbonic clearance is represented with phagocytic index. It is calculated as follows phagocytic index a:
Table 1 test packet and medicining condition
| Sequence number | Group | Number/only | Medicine | Dosage |
| 1 | 1st group | 10 | The embodiment of the present invention 1, gastric infusion, it is used in conjunction 7 days | 0.8g/kg |
| 2 | 2nd group | 10 | The embodiment of the present invention 2, gastric infusion, it is used in conjunction 7 days | 0.8g/kg |
| 3 | 3rd group | 10 | The embodiment of the present invention 3, gastric infusion, it is used in conjunction 7 days | 0.8g/kg |
| 4 | 4th group | 10 | The embodiment of the present invention 4, gastric infusion, it is used in conjunction 7 days | 0.8g/kg |
| 5 | 5th group | 10 | The embodiment of the present invention 5, gastric infusion, it is used in conjunction 7 days | 0.8g/kg |
| 6 | 6th group | 10 | Astragalus polysaccharide extract, gastric infusion, it is used in conjunction 7 days | 0.8g/kg |
| 7 | 7th group | 10 | Normal saline, gavage, it is used in conjunction 7 days |
3 results and analysis
Concrete outcome is in Table 2.In phagocytic index a, compared with the 7th group of blank, other is respectively organized, and all there were significant differences (P<0.05), and wherein the 1st, 5 groups have pole significant difference (P<0.01). (P>0.05) all it is not significantly different between 1-6 group; In index and spleen index, compared with the 7th group of blank, except the 5th group, there were significant differences (P=0.012<0.05), and other each group is all not significantly different from (P>0.05); In liver index, between each group, all it is not significantly different from (P>0.05).
Table 2 result of the test
| Group | A value | Index and spleen index | Liver index |
| 1st group | 7.37��0.31A | 0.4035��0.0776 | 5.3050��0.3511 |
| 2nd group | 7.03��0.51a | 0.3909��0.0381 | 5.2854��0.2334 |
| 3rd group | 6.98��0.43a | 0.4049��0.1228 | 5.2655��0.5007 |
| 4th group | 7.09��0.44a | 0.4061��0.0790 | 5.3817��0.4868 |
| 5th group | 7.35��0.38A | 0.4257��0.0605a | 5.4604��0.4123 |
| 6th group | 7.06��0.54a | 0.3906��0.0801 | 5.4783��0.4608 |
| 7th group | 6.27��0.35b | 0.3473��0.0260b | 5.3653��0.2538 |
Note: 1) represent significant difference between different lower cases, represent between different size letter that difference is extremely notable; 2), on phagocytic index a, compared with the 7th group of blank group, 1-6 group P value is respectively as follows: 0.001,0.031,0.034,0.021,0.005,0.025.
4 conclusions
Embodiment of the present invention 1-5 herbal mixture pharmaceutical composition can significantly improve mice carbonic clearance ability, and to a certain degree improves the index and spleen index of mice, illustrates that embodiment of the present invention 1-5 herbal mixture pharmaceutical composition can improve mouse monokaryon-macrophage function.
The immunoenhancement result of mice is tested by test example 2 embodiment of the present invention
By observing the impact on mouse immune index power of the embodiment of the present invention herbal mixture pharmaceutical composition, investigate the immunoenhancement result of embodiment of the present invention herbal mixture pharmaceutical composition.
1 material
Trial drug: embodiment of the present invention 6-8 herbal mixture pharmaceutical composition; Control drug: astragalus polysaccharide extract.
Experimental animal: BALB/C mice, male, 150,16��18g. Purchased from Beijing Vital River Experimental Animals Technology Co., Ltd..
2 methods
Test sets 5 groups altogether, often 20 mices of group. 1-3 group is administered embodiment of the present invention 6-8 herbal mixture pharmaceutical composition successively, and dosage is 0.8g/kg body weight; 4th group of administration astragalus polysaccharide extract, dosage 0.8g/kg body weight; 5th group is negative control group, gavage normal saline. It is used in conjunction 7 days. During test free choice feeding drinking-water, every day observed and recorded mice situation. Test packet situation is in Table 3.
Packet situation tested by table 3
| Group | Medicine | Administering mode and dosage | Quantity |
| 1st group | The embodiment of the present invention 6 | Gastric infusion, 0.8g/kg, it is used in conjunction 7 days | 20 |
| 2nd group | The embodiment of the present invention 7 | Gastric infusion, 0.8g/kg, it is used in conjunction 7 days | 20 |
| 3rd group | The embodiment of the present invention 8 | Gastric infusion, 0.8g/kg, it is used in conjunction 7 days | 20 |
| 4th group | Astragalus polysaccharide extract | Gastric infusion, 0.8g/kg, it is used in conjunction 7 days | 20 |
| 5th group | Normal saline | Gavage, is used in conjunction 7 days | 20 |
3 testing indexs
Organ index measures: test the 8th day, often organizes and takes the disconnected neck execution of 10 mices at random, takes thymus and spleen is weighed, calculate organ index, respectively organize the difference of mice organs index.
Thymus index=chest gland weight/body weight �� 100%
Index and spleen index=spleen weight/body weight �� 100%
Cellular immune function assay
Spleen lymphocyte conversion ratio measures: test the 8th day, often organizes and takes the disconnected neck of 6 mices at random and put to death, and the aseptic spleen that takes is put into and filled in appropriate aseptic Hank ' s liquid plate, gently by spleen grinding, makes individual cells suspension. Filter through 200 eye mesh screens, wash 2 times with Hank ' s liquid, centrifugal (1000r/ minute, 10 minutes), then by cell suspension in the complete culture solution of 2ml, count splenocyte with full-automatic cell calculating instrument, adjusting cell concentration is 3 �� 106/ml��
Cell suspension point holes being added in 24 well culture plates, every hole 1ml, a hole adds 75 �� lConA liquid, and 5%CO, as comparison, is put in another hole2, 37 DEG C of CO2Incubator is cultivated 72h.Often group set 3 parallel. Cultivation terminates front 4h, and every hole sucks supernatant 0.7ml gently, adds the 0.7ml RPMI1640 culture fluid without calf serum, is simultaneously introduced MTT (5mg/ml) 50 �� l/ hole, continues to cultivate 4h. After cultivation terminates, every hole adds 1ml acid isopropyl alcohol, piping and druming mixing, makes purple crystal be completely dissolved. Then being dispensed in 96 well culture plates, subpackage 3 hole, each hole, as Duplicate Samples, with enzyme-linked immunosorbent assay instrument, measures optical density value with 570nm wavelength.
4 results and analysis
4.1 organ index measurement results
Organ coefficient measurement result is in Table 4. With Spss18.0 software, each group of data are carried out one factor analysis of variance. Result of the test shows, each group mouse thymus index is all not significantly different from (P>0.05), and there were significant differences for index and spleen index (P<0.05). 1st group with the 4th, 5 groups all have pole significant difference (P<0.01), the 2nd, 3 groups respectively with the 4th, 5 groups all there were significant differences (P<0.05). Illustrate that successive administration embodiment of the present invention 6-8 herbal mixture pharmaceutical composition can improve the index and spleen index of mice.
Table 4 Thymus and Spleen index data statistics
| Group | Thymus index | Index and spleen index |
| 1st group | 0.2672��0.0595 | 0.5002��0.1228A |
| 2nd group | 0.2730��0.0373 | 0.4637��0.1012a |
| 3rd group | 0.2473��0.0341 | 0.4477��0.0773a |
| 4th group | 0.2419��0.0477 | 0.3712��0.0489b |
| 5th group | 0.2535��0.0462 | 0.3727��0.0276b |
Note: (1) identical letter represents does not have difference, different lower cases to represent each group difference notable (P < 0.05), different size lowercase alphabet differential heteropole notable (P < 0.01) between each group; (2) compared with the 5th group, 1-4 group index and spleen index P value difference 0.001,0.018,0.049 and 0.968.
4.2 cellular immune function assay results
Spleen lymphocyte conversion ratio result of the test is in Table 5. With Spss18.0 software, each group of optical density difference (OD difference) is carried out one factor analysis of variance. Result of the test shows, 1-4 group all exists significant difference (P < 0.05) with the 5th group in OD difference, and wherein the 1st, 2 and 4 groups exist pole significant difference (P < 0.01) with the 5th group. After administration embodiment of the present invention 6-8 herbal mixture pharmaceutical composition is described, mouse spleen lymphocyte competence for added value strengthens, and is administered a degree of cellular immune function that improve mice of embodiment of the present invention 6-8 herbal mixture pharmaceutical composition.
Table 5 spleen lymphocyte conversion ratio measurement result (MMT method)
| Group | It is not added with ConA OD value | Add ConA OD value | OD difference |
| 1st group | 0.2473��0.0043 | 0.2658��0.0039 | 0.0185��0.0053a |
| 2nd group | 0.2600��0.0061 | 0.3195��0.0093 | 0.0595��0.0135A |
| 3rd group | 0.2448��0.0049 | 0.3923��0.0076 | 0.1475��0.0052A |
| 4th group | 0.2670��0.0028 | 0.4143��0.0111 | 0.1473��0.0119A |
| 5th group | 0.3083��0.0049 | 0.3108��0.0051 | 0.0025��0.0052b |
Note: (1) identical letter represents does not have difference, different lower cases to represent each group difference notable (P < 0.05), different size lowercase alphabet differential heteropole notable (P < 0.01) between each group; (2) compared with the 5th group, 1-4 group OD difference P value difference 0.024,0.000,0.000 and 0.000.
5 conclusions
Embodiment of the present invention 6-8 herbal mixture pharmaceutical composition can significantly improve mouse spleen lymphocyte conversion ratio, and to a certain degree improves the index and spleen index of mice, illustrates that mice is had immunoenhancement result by embodiment of the present invention herbal mixture pharmaceutical composition.
Test example 3 embodiment of the present invention experiment of preventive effects to coli-infection chickling
In order to observe embodiment of the present invention herbal mixture pharmaceutical composition to the colibacillary preventive effect of Chickens Infected, 15 ages in days sea blue ash chickling is carried out artificial challenge's escherichia coli prophylactic tria by us.
1 material
Trial drug: embodiment of the present invention 3-5 herbal mixture pharmaceutical composition; Control drug: astragalus polysaccharide extract.
Experimental animal: 15 Japanese instar chicklings, 180, the blue ash in sea, purchased from close kind of chicken hatchery of system in Beijing Jing.
Counteracting toxic substances strain: fowl E.coli O1Type reference culture (CVCC249), purchased from China Veterinary Drugs Supervisory Inst..
2 methods
Test sets 6 groups altogether, often group 30. 1-3 group is administered embodiment of the present invention 3-5 herbal mixture pharmaceutical composition, drinking water administration successively, and dosage is 4g/L; 4th group of administration astragalus polysaccharide extract, drinking water administration, dosage is 4g/L; 5th group is positive controls, is not administered; 6th group is negative control group, is not administered. Test packet situation is specifically shown in table 6. Successive administration 7 days, within the 8th day, except negative control group, other respectively organizes lumbar injection escherichia coli suspension, every 0.5ml (1 �� 106Cfu/ml). Continuous observation 14 days after counteracting toxic substances infection, observed and recorded chickling morbidity every day death condition. During test, free choice feeding is drunk water.
Table 6 test packet
3 results and analysis
3.1 survival condition
Survival condition is in Table 7. Find through X 2 test (SPSS18.0) statistical analysis, in survival rate, compared with the 5th group of positive controls, 2nd, 3 groups all there were significant differences (P<0.05), 1st, 4 groups are all not significantly different from (P>0.05), but the 1st, 4 groups of amount of survivals are higher than the 5th group, are not significantly different between each group of 1-4 group. In weightening finish, compared with the 5th group of positive controls, other is respectively organized, and all there were significant differences (P<0.05), and wherein the 6th group has pole significant difference (P<0.01).
Table 7 survival condition table
Note: identical letter represents does not have difference, different lower cases to represent each group difference notable (P < 0.05), different size lowercase alphabet differential heteropole notable (P < 0.01) between each group.
3.2 pathological changes situations
After off-test, residue chickling is all put to death by we, and anatomic observation pathological change, statistical analysis pathological changes situation, concrete outcome is in Table 8. Finding through SPSS18.0 statistical analysis, the comprehensive score aspect of pathological changes, compared with the 5th group of positive controls, 1-4 group all has pole significant difference (P < 0.01); Heart change score aspect, compared with the 5th group of positive controls, all there were significant differences for 1-4 group (P < 0.05), and wherein the 2nd, 3 groups have pole significant difference (P < 0.01); Hepatic lesions score aspect, compared with the 5th group of positive controls, all there were significant differences for 1-4 group (P < 0.05), and wherein the 3rd, 4 groups have pole significant difference (P < 0.01).
Table 8 pathological changes situation
| Group | Quantity/plumage | Comprehensive score | The heart | Liver | Lung | Air bag |
| 1st group | 17 | 5.92��4.17A | 2.31��1.80a | 2.54��1.85a | 0.23��0.44a | 0.85��1.28 |
| 2nd group | 19 | 5.00��4.31A | 1.91��1.92A | 2.00��1.73a | 0.55��0.52 | 0.55��0.93a |
| 3rd group | 19 | 4.29��4.21A | 2.00��1.70A | 0.94��1.82A | 0.53��0.80 | 0.82��1.13 |
| 4th group | 16 | 6.00��2.80A | 2.54��1.45a | 1.62��0.96A | 0.54��0.52 | 1.31��1.25 |
| 5th group | 10 | 11.80��3.94b | 4.40��1.58b | 4.60��1.58b | 1.10��1.10b | 1.70��1.70b |
| 6th group | 30 | 0 | 0 | 0 | 0 | 0 |
Note: identical letter represents does not have difference, different lower cases to represent each group difference notable (P < 0.05), different size lowercase alphabet differential heteropole notable (P < 0.01) between each group.
5 conclusions
Embodiment of the present invention 3-5 herbal mixture pharmaceutical composition can significantly improve chickling resistance against diseases, and successive administration postoperative infection escherichia coli survivability of chicks is significantly improved; In addition successive administration embodiment of the present invention 3-5 herbal mixture pharmaceutical composition can promote the recovery of the weight recovery after Chickens Infected escherichia coli and diseased organ, illustrate that embodiment of the present invention herbal mixture pharmaceutical composition can strengthen the immunocompetence of chickling, improve the resistance against diseases of chickling.
The test example 4 embodiment of the present invention 5 herbal mixture pharmaceutical composition experiment of preventive effects to diarrhea of weaned piglets
Test carries out on Beijing Huairou pig farm, and selected from Du �� length �� Dasanyuan first-filial generation newborn piglet that source is consistent, growth promoter is normal, is in a good state of health. , sex, body weight other according to nest are divided into test group, matched group and reference group by paired experiment principle.The use that reference group compares each other as test group, matched group, often organizes 3 nest piglets, numbering before test. Difference drinking water administration before wean, the test group administration embodiment of the present invention 5 herbal mixture pharmaceutical composition, by 0.8g/kg, once a day, it is used in conjunction 7 days; Matched group administration astragalus polysaccharides, by 0.8g/kg, once a day, is used in conjunction 7 days; Reference group is not administered. Wean after drug withdrawal, continue to observe 2 weeks. Normally feed during test, record piglet situation every day.
Result of the test is in Table 9. In process of the test, reference group-1 has 1 piglets because of diarrhoea Serious Mortality the 2nd week (during wean), and reference group-2 has 2 pigletss because of diarrhoea Serious Mortality, and other is respectively organized the phenomena of mortality does not all occur. In piglet diarrhea, test group and matched group diarrhoea situation are lighter, through X 2 test (SPSS18.0 statistical software), compared with reference group, test group and matched group all have pole significant difference (P < 0.01), show that the administration embodiment of the present invention 5 herbal mixture pharmaceutical composition improves the immunity of piglet, enhance resistance against diseases and anti-stress ability, significantly reduce piglet diarrhea rate.
Table 9 logging
Note: 1) diarrhoea head, observing 1 piglets the same day has the meter 1 of diarrhoea; 2) * indicates that piglets is because of diarrhoea Serious Mortality; 3) identical letter represents does not have difference, different lower cases to represent each group difference notable (P < 0.05), different size lowercase alphabet differential heteropole notable (P < 0.01) between each group.
The above is only the preferred embodiment of the present invention; it should be pointed out that, for those skilled in the art, under the premise without departing from the technology of the present invention principle; can also making some improvements and modifications, these improvements and modifications also should be regarded as protection scope of the present invention.
Claims (8)
1. a herbal mixture pharmaceutical composition for Fuzheng Jiedu, it contains active component, and described active component is made up of the Radix Astragali, Radix Angelicae Sinensis, Echinacea, Poria, the Rhizoma Atractylodis Macrocephalae, Radix Isatidis, Folium Isatidis, Herba Epimedii, Radix Glycyrrhizae.
2. herbal mixture pharmaceutical composition according to claim 1, it is characterized in that, described active component by weight, is made up of Radix Astragali 1-50 part, Radix Angelicae Sinensis 1-20 part, Echinacea 1-80 part, Poria 1-20 part, Rhizoma Atractylodis Macrocephalae 1-20 part, Radix Isatidis 1-40 part, Folium Isatidis 1-40 part, Herba Epimedii 1-30 part, Radix Glycyrrhizae 1-20 part.
3. herbal mixture pharmaceutical composition according to claim 2, it is characterized in that, described active component by weight, is made up of Radix Astragali 5-40 part, Radix Angelicae Sinensis 2-15 part, Echinacea 10-70 part, Poria 2-15 part, Rhizoma Atractylodis Macrocephalae 2-15 part, Radix Isatidis 5-30 part, Folium Isatidis 5-30 part, Herba Epimedii 3-20 part, Radix Glycyrrhizae 2-15 part.
4. herbal mixture pharmaceutical composition according to claim 3, it is characterized in that, described active component by weight, is made up of Radix Astragali 10-30 part, Radix Angelicae Sinensis 3-10 part, Echinacea 30-60 part, Poria 3-10 part, Rhizoma Atractylodis Macrocephalae 3-10 part, Radix Isatidis 10-20 part, Folium Isatidis 10-20 part, Herba Epimedii 5-15 part, Radix Glycyrrhizae 3-10 part.
5. the herbal mixture pharmaceutical composition described in claim 1 ~ 4, it is characterised in that it is powder, granule, tablet, oral liquid or injection.
6. a preparation method for the herbal mixture pharmaceutical composition described in any one of claim 1 ~ 5, it comprises the steps:
Weigh each medical material by weight, pulverize, add 8-10 times amount soak by water 1-3 time, each 1-2h, merge decoction liquor, filter, filtrate is concentrated in right amount, adds 1-3 times of dehydrated alcohol and staticly settles overnight, filters, filtrate is concentrated into extractum shape, and corresponding dosage form made by the corresponding pharmaceutically acceptable carrier of dried addition or adjuvant.
7. the herbal mixture pharmaceutical composition described in any one of claim 1 ~ 5 is being prepared Fuzheng Jiedu, strengthening cultivated animals immunity of organisms and is being improved the application in the medicine of cultivated animals premunition.
8. contain the feedstuff of herbal mixture pharmaceutical composition described in any one of claim 1 ~ 5.
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| CN106389788A (en) * | 2016-11-14 | 2017-02-15 | 青岛农业大学 | Medicine extract for strengthening anti-virus capability and immune function of livestock, preparation, and preparation method |
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| CN108887473A (en) * | 2018-06-07 | 2018-11-27 | 广东鹏汇生物科技有限公司 | A kind of natural plant feed additives and preparation method thereof |
| CN111466493A (en) * | 2020-03-20 | 2020-07-31 | 四川恺多萌宠文化传播有限公司 | Health care nutrition composition for pets and preparation method thereof |
| CN111643579A (en) * | 2020-07-27 | 2020-09-11 | 郑州市旭源生物科技有限公司 | Traditional Chinese medicine composition for preventing and treating porcine circovirus disease and preparation method and application thereof |
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Application publication date: 20160608 |