CN103735608A - Traditional Chinese medicine composition as well as preparation method and application thereof - Google Patents
Traditional Chinese medicine composition as well as preparation method and application thereof Download PDFInfo
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Abstract
The invention discloses a traditional Chinese medicine composition as well as a preparation method and application thereof. The traditional Chinese medicine composition is prepared from the following crude drugs in parts by weight: 50-200 parts of astragalus membranaceus, 30-200 parts of herba epimedii and 40-150 parts of echinacea. The traditional Chinese medicine composition disclosed by the invention exerts the combination effects of diagnosis and treatment of the traditional Chinese medicine prescription, is good in curative effect, fast to take effect, and environmental friendly, free of damages of drug residues, small in side effect, simple in preparation technology, low in cost, abundant in raw material source, and beneficial to wide popularization and application, does not easily generate tolerance, can be applied to prevention and treatment of immunosuppression syndrome of a pig, and has strong market competitiveness.
Description
Technical field
The present invention relates to field of veterinary, relate in particular to a kind of Chinese medicine composition and its preparation method and application.
Background technology
Pig immunosuppressant (Immune inhibition or Immunity Suppression) refers to that the immune system in pig body sustains damage or destroys, cause the temporary or permanent immunodeficiency disease of body, be reactive depression or the disappearance that body stimulates antigenic substance, pig is improved the sensitivity of disease.
At present, the factor that causes pig immunosuppressant and immuning failure is more and more, is broadly divided into infectious factors and non-infectious factor two aspects, and these factors cause body to reduce exotic antigen resistance, increase sickness rate and treatment difficulty, to Swine Production, brought very large economic loss.Verified have the pathogen of inhibitive ability of immunity to have porcine reproductive and respiratory syndrome virus (PRRSV), porcine circovirus 2 types (PCV-2), swine influenza virus (SIV), swine fever virus (HCV), pseudorabies virus (PRV), porcine parvovirus (PPV), African swine fever virus (ASFV), mycoplasma pneumoniae (MHP), Eperythrozoon (Eperythrozoon), Actinobacillus pleuropneumoniae (APP) etc. to pig, and different pathogens disturbs acknowledgement mechanism as follows to immune system:
Porcine reproductive and respiratory syndrome virus (PRRSV) makes lymph node, lymphatic nodule and paracortical area and splenic white pulp and red pulp all be subject to the destruction of popularity, cause humoral immunization and the cell immunocompetent of body to reduce, produce immunosuppressant and immune interference, thereby other pathogen infections of secondary, particularly encroach on the pathogenic microorganism of respiratory system, be mainly pasteurella multocida, Streptococcus suis, the secondary haemophilus of pig and Salmonella etc., cause higher M & M.
Sick 2 types of porcine circovirus (PCV-2) are mainly invaded in affected pig lungs, thymus, spleen, cause B, T lymphocyte quantity in circulation B cell and T cell and lymphatic organ to reduce and atrophy, thereby cause immunosuppressant to occur.Can cause multisystemic exhaustion syndrome (PMWS) after pig wean, newborn piglet is congenital trembles (CT), pig hypertrophy and necrotizing pneumonia (PNP), pig interstitial pneumonia (IP), Corii Sus domestica inflammation and nephrotic syndrome (PDNS), porcine respiratory syndrome (PRDC) and sow breeding difficulty etc.PCV-2 infects pig farm and also easily causes the generation of Other diseases, as pseudorabies, PRRS, pig parvoviral, swine flue, pig streptococcicosis, porcine salmonellosis, colibacillosis of pigs, trophism hepatosis and suppurative bronchopneumonia etc.
HCV has special preferendum to the lymphocyte of pig.HCV infection causes occurring congestive hemorrhage and the infarction of spleen of skin, mucosa, internal organs and lymphocyte is decayed, T cytoactive is suppressed, and follows the decline of lymphatic organ and bone marrow to change.Lymphoid damage and fracture causes Acquired immunodeficient state to produce, thereby will make pig generation superinfection cause that Mortality number increases severely.
SIV can cause the immune organ major injury of pig, SIV easily causes the intrusion of other cause of diseases to the pathological lesion of respiratory apparatus, as PRRSV, PRCV (Porcine Respiratory Coronavirus), pig pleuropneumonia actinomycetes, bronchus deteriorated blood bordetella bacilli, pasteurella multocida, the secondary haemophilus of pig, Streptococcus suis, escherichia coli etc., the harm of secondary infection more merits attention.
In sum, most pathogen be except doing disturbance thing immunne response, cause outside intrinsic disease itself, the more important thing is and can cause the particularly secondary infection (antibacterial, virus, fungus and parasite etc.) of piglet and growth swinery of swinery.Except infectious factors, the non-infectious factors such as antibiotics, stress, irrational feeding and management are also that pig body produces immunosuppressant factor.Antibiotics is curative drug, but also has an immunosuppressive factor simultaneously: as chloromycetin can change the function of gut associated lymphatic tissue; Chlortetracycline can cause the obvious damage of gut associated lymphatic tissue; Oxytetracycline can suppress leukocytic division etc., and these can cause the reduction of body's immunity.Evidence, the animal that takes antibiotics, inoculates vaccine, and the antibody horizontal producing can obviously reduce.In addition, the halogens such as the heavy metal substances such as lead, cadmium, hydrargyrum and iodine, are also to cause immunosuppressant important factor, and its reason is that the T, the B immunocyte that make body form antibody reduce, thereby reduce the ability of immune response reaction.
In swinery, ubiquity be take immunosuppressant virus and is that main various immunosuppressive agents are not isolated existence, they are often mutually promoted, reciprocal causation, it is very complicated that thereby the cause of disease that makes various diseases becomes, the immunosuppressant of its initiation, caused Swinery immunity power and health level to decline, the susceptibility of disease is increased, this is also the basic reason place that in current Swine Production, swine diseases is difficult to diagnosis and control.Prevention and treatment pig immunosuppressant disease, except carrying out scientific and reasonable immunization according to each pig farm, can also be used 1,3-1, the conventional Western medicine control such as 6-callose, oligosaccharide, yeast cell wall, tilmicosin.But vaccine itself has immunosuppressive action to pig body, be more prone to cause secondary infection, and Western medicine easily causes the residue of veterinary drug in product to exceed standard, easily produce drug resistance, therefore with needing certain off-drug period to guarantee the safety of livestock products after Western medicine.
Have at present Chinese herbal medicine is applied in Swine Production to reach the immunity that improves pig body, the effect of disease preventing and treating, Chinese medicine preparation can be avoided the problems such as Western medicine side effect is large, drug residue harm, and therefore, the Chinese medicine preparation of developing a kind of immunosuppressant syndrome for pig seems particularly important.
Summary of the invention
In order to overcome the weak point of the prior art of above-mentioned indication, the invention provides a kind of good effect, no drug residue and drug resistance, and there is Chinese medicine composition strengthening immunologic function effect and its preparation method and application, effectively to prevent and treat pig immunosuppressant syndrome.
The present invention is achieved by the following technical solutions:
A Chinese medicine composition, described Chinese medicine composition is prepared from by the crude drug in whole of following parts by weight proportioning: 50～200 parts of the Radixs Astragali, 30～200 parts of Herba Epimedii, 40～150 parts of Echinaceas.
Preferably, described Chinese medicine composition is prepared from by the crude drug in whole of following parts by weight proportioning: 70～200 parts of the Radixs Astragali, 40～180 parts of Herba Epimedii, 40～130 parts of Echinaceas.
Preferably, described Chinese medicine composition is prepared from by the crude drug in whole of following parts by weight proportioning: 120 parts of the Radixs Astragali, 60 parts of Herba Epimedii, 60 parts of Echinaceas.
The present invention selects the effect of raw material to be summarized as follows:
The Radix Astragali: sweet, temperature.Return lung, spleen channel.Invigorating QI to consolidate the body surface resistance, diuresis poison holding, evacuation of pus, expelling pus and promoting granulation.Weak for the deficiency of vital energy, anorexia and loose stool, sinking of QI of middle-JIAO, chronic diarrhea proctoptosis, the metrorrhagia of having blood in stool, exterior deficiency spontaneous perspiration, deficiency of vital energy edema, carbuncle is thwarted routed, and burst and do not hold back for a long time, blood deficiency dull yellowish colored skin, interior-heat is quenched one's thirst; Chronic nephritis proteinuria, diabetes.
Herba Epimedii: acrid, sweet, warm.Return liver, kidney channel.Kidney-replenishing, bone and muscle strengthening, wind-damp dispelling.For impotence and seminal emission, muscles and bones flaccidity is soft, rheumatic arthralgia, numbness contracture; Climacteric hypertension.
Echinacea: contain the various bioactivators such as polysaccharide, glycoprotein, caffeic acid and chicoric acid, there is immunostimulant, antiinflammatory, antibacterial, Anti-bacterium and antineoplastic action.Echinacea is the internationally recognized degree of depth immune material with " bone marrow preservation " effect.
According to Chinese veterinarian monarch, minister, the principles of formulating prescriptions helping, make, in side, the Radix Astragali has tonifying Qi and lifting yang, benefiting QI for strengthening the superficies, diuresis poison holding, expelling pus and promoting granulation, have enhancing human body immunity, heart tonifying, promotion bone marrow hematogenesis, protect the liver, the effect such as diuresis, the various antibacterials of inhibition and virus, be the principal agent in side.Herba Epimedii has the effect that strengthens gonad, enhancing human body immunity, promotion osteogenesis, has the effect of kidney-replenishing, bone and muscle strengthening, wind-damp dispelling; Echinacea has the autonomous immunity of raising, promotes tissue growth and anti-infectious function, is the ministerial drug in side altogether.Three medicines share, and have tonifying Qi and lifting yang, significantly improve the effect of immunity, mainly for pig immunosuppressant syndrome disease, have good efficacy.
The dosage form of described Chinese medicine composition is any dosage form in powder, tablet, powder, granule, oral liquid, injection.
The preparation method that the invention also discloses the powder of above-mentioned Chinese medicine composition, comprises the following steps:
A, after being weighed by proportioning, each crude drug in whole pulverizes;
B, after being crossed to 24～100 mesh sieves, crude drug in whole is fully mixed with into powder.
The invention also discloses powder, the tablet of above-mentioned Chinese medicine composition, the preparation method of granule, comprise the following steps:
A, each crude drug in whole weighed, pulverized by proportioning and mix after, with 30～60 minutes post-heating of water soaking crude drug in whole of 6～10 times of weight, to boiling, extract, extraction time is twice, boiling time is 1～3 hour;
B, filter after, merging filtrate the thick paste shape that relative density is 1.20～1.40 when filtrate is concentrated into 80 ℃, preferably, the thick paste shape that while being concentrated into 80 ℃, relative density is 1.30～1.35;
C, the product of concentrated gained is added to conventional adjuvant, according to common process, be prepared into required dosage form.
The required dosage form of the step c of said method comprises powder, tablet, granule.The described conventional adjuvant of step c comprises any one or more of starch, dextrin, sucrose, magnesium stearate.
The preparation method that the invention also discloses oral liquid or the injection of above-mentioned Chinese medicine composition, comprises the following steps:
A, each crude drug in whole weighed, pulverized by proportioning and mix after, with 30～60 minutes post-heating of water soaking crude drug in whole of 6～10 times of weight, to boiling, extract, extraction time is twice, boiling time is 1～3 hour;
B, filter after, merging filtrate is also concentrated into every 1ml and is equivalent to crude drug in whole 1～3g;
C, to adding 1.5～2.5 times of amount 75% ethanol in concentrated filtrate, stir, standing waiting precipitated, leaching supernatant reclaims ethanol, surplus liquid is concentrated into every 1ml and is equivalent to crude drug in whole 4～6g, is pulpous state;
D, according to common process, be prepared into oral liquid or injection.
Chinese medicine composition of the present invention is applied in the immunosuppressant syndrome medicament of preparation control pig, can be used for preparation control pig immunosuppressant syndrome medicament; Also can add in feedstuff, for the preparation of the feedstuff of control pig immunosuppressant syndrome.Be 3～7 days a course for the treatment of of described Chinese medicine composition, using dosage be every day oral dose be 0.1～0.3g/ kg body weight, be used in conjunction 10～15 days; Injection volume is each 0.1～0.2ml/ kg body weight, and injection on the one 2 times, is used in conjunction 3～7 days.
In the present invention, every 1ml finished product is equivalent to crude drug in whole 1～3g and refers to that every 1ml finished product need to utilize 1～3g crude drug in whole to be prepared from; Every 1g finished product is equivalent to crude drug in whole 1g and refers to that every 1g finished product need to utilize 1g crude drug in whole to be prepared from.
Compared with prior art, advantage and the effect of Chinese medicine composition of the present invention comprise: the combined effect of (1) performance Chinese medicinal formulae determination for the treatment of based on pathogenesis obtained through differentiation of symptoms and signs, good effect, instant effect, environmental protection, drug residue free endanger, side effect is little, be difficult for producing drug resistance, can be used for preventing and treating the immunosuppressant syndrome of pig; (2) preparation technology is simple, cost is low, has the stronger market competitiveness; (3) raw material of Chinese medicine source is abundant, and moderate cost, is conducive to wide popularization and application.
The specific embodiment
For the ease of those skilled in the art's understanding, below in conjunction with embodiment, the invention will be further described.
Embodiment 1
A Chinese medicine composition, the crude drug in whole proportioning of described Chinese medicine composition is: Radix Astragali 200g, Herba Epimedii 200g, Echinacea 150g, parts by weight are 200 parts of the Radixs Astragali, 200 parts of Herba Epimedii, 150 parts of Echinaceas.
The preparation method of above-mentioned Chinese medicine composition, comprises the following steps:
A, after being weighed by proportioning, each crude drug in whole pulverizes;
B, after being crossed to 24 mesh sieves, crude drug in whole is fully mixed with into powder.
The Chinese medicine composition of described powder type is applied to prevent and treat pig immunosuppressant syndrome, and its using method is mixed feeding: every 1kg feedstuff adds this Chinese medicine composition 5g, uses continuously 10～15 days.
Embodiment 2
A Chinese medicine composition, the crude drug in whole proportioning of described Chinese medicine composition is: Radix Astragali 50g, Herba Epimedii 30g, Echinacea 40g, parts by weight are 50 parts of the Radixs Astragali, 30 parts of Herba Epimedii, 40 parts of Echinaceas.
The preparation method of above-mentioned Chinese medicine composition, comprises the following steps:
A, each crude drug in whole weighed, pulverized by proportioning and mix after, with 30 minutes post-heating of water soaking crude drug in whole of 6 times of weight, to boiling, extract, extraction time is twice, primary extraction time is 1.5 hours, primary extraction time is 1 hour;
B, filter after, merging filtrate the thick paste shape that relative density is 1.30～1.35 while being concentrated into 80 ℃;
C, the product of concentrated gained is added to appropriate amount of starch, dextrin or other conventional adjuvant, mix homogeneously, dries, and pulverizes, and is prepared into the powder that every 1g finished product is equivalent to 1g crude drug in whole.
The Chinese medicine composition of described powder-type is applied to prevent and treat pig immunosuppressant syndrome, and its using method is mixed feeding: every 1kg feedstuff adds this Chinese medicine composition 2g, is used in conjunction 10～15 days.
Embodiment 3
A Chinese medicine composition, the crude drug in whole proportioning of described Chinese medicine composition is: Radix Astragali 120g, Herba Epimedii 60g, Echinacea 60g, parts by weight are 120 parts of the Radixs Astragali, 60 parts of Herba Epimedii, 60 parts of Echinaceas.
The preparation method of above-mentioned Chinese medicine composition, comprises the following steps:
A, each crude drug in whole weighed, pulverized by proportioning and mix after, with 60 minutes post-heating of water soaking crude drug in whole of 10 times of weight, to boiling, extract, extraction time is twice, primary extraction time is 3 hours, primary extraction time is 1 hour;
B, filter after, merging filtrate the thick paste shape that relative density is 1.30～1.35 while being concentrated into 80 ℃;
C, the product of concentrated gained is added to appropriate amount of starch, dextrin or other conventional adjuvant, mix homogeneously, makes soft material, granulates, and dries, and is prepared into the granule that every 1g finished product is equivalent to crude drug in whole 1g.
The Chinese medicine composition of described granule dosage form is applied to prevent and treat pig immunosuppressant syndrome, and its using method is mixed feeding: every 1kg feedstuff adds this Chinese medicine composition 2g, and free choice feeding is used in conjunction 10～15 days.
Embodiment 4
A Chinese medicine composition, the crude drug in whole proportioning of described Chinese medicine composition is: Radix Astragali 150g, Herba Epimedii 60g, Echinacea 40g, parts by weight are 150 parts of the Radixs Astragali, 60 parts of Herba Epimedii, 40 parts of Echinaceas.
The preparation method of above-mentioned Chinese medicine composition, comprises the following steps:
A, each crude drug in whole weighed, pulverized by proportioning and mix after, with 30 minutes post-heating of water soaking crude drug in whole of 6 times of weight, to boiling, extract, extraction time is twice, primary extraction time is 1.5 hours, primary extraction time is 1 hour;
After b, filtration, merging filtrate is also concentrated into 250ml;
C, to adding 2 times of amount 75% ethanol in concentrated filtrate, stir, standing waiting precipitated, leaching supernatant reclaims ethanol, surplus liquid is concentrated into every 1ml and is equivalent to crude drug in whole 5g, is pulpous state;
D, by step c gained concentrated solution through ethanol extraction, filtration, fine straining, embedding, sterilizing, be prepared into the injection that every 1ml finished product is equivalent to crude drug in whole 1g.
The Chinese medicine composition of described injection type is applied to prevent and treat pig immunosuppressant syndrome, and its using method is intramuscular injection: every 1kg weighing machine, each injection volume is 0.1～0.2ml/kg, 2 times on the one, is used in conjunction 3～5 days.
Embodiment 5
A Chinese medicine composition, the crude drug in whole proportioning of described Chinese medicine composition is: Radix Astragali 100g, Herba Epimedii 60g, Echinacea 130g, parts by weight are 100 parts of the Radixs Astragali, 60 parts of Herba Epimedii, 130 parts of Echinaceas.
A, each crude drug in whole weighed, pulverized by proportioning and mix after, with 60 minutes post-heating of water soaking crude drug in whole of 10 times of weight, to boiling, extract, extraction time is twice, primary extraction time is 3 hours, primary extraction time is 1 hour;
After b, filtration, merging filtrate is also concentrated into 250ml;
C, in concentrated filtrate, add 2 times of 75% ethanol, stir, standing waiting precipitated, and after filtration, the method that filtrate is refluxed by steaming and decocting reclaims ethanol, and reconcentration to every 1ml concentrated solution is equivalent to primary crude drug 1g;
D, by step c gained concentrated solution after filtration, embedding, sterilizing, be prepared into oral liquid.
The Chinese medicine composition of described oral liquid formulation is applied to prevent and treat pig immunosuppressant syndrome, and its using method is mixed drink: in every 1L water, add this Chinese medicine composition Chinese medicine 2ml, freely drink, be used in conjunction 10～15 days.
Embodiment 6
A Chinese medicine composition, the crude drug in whole proportioning of described Chinese medicine composition is: Radix Astragali 140g, Herba Epimedii 80g, Echinacea 80g, parts by weight are 140 parts of the Radixs Astragali, 80 parts of Herba Epimedii, 80 parts of Echinaceas.
The preparation method of above-mentioned Chinese medicine composition, comprises the following steps:
A, each crude drug in whole weighed, pulverized by proportioning and mix after, with 40 minutes post-heating of water soaking crude drug in whole of 8 times of weight, to boiling, extract, extraction time is twice, primary extraction time is 1.5 hours, primary extraction time is 1 hour;
After b, filtration, the thick paste shape that when merging filtrate also concentrates 80 ℃, relative density is 1.30～1.35;
C, the product of concentrated gained is added to starch, magnesium stearate, granulate, tabletting, make every 0.2g, every 1g finished product is equivalent to the tablet of crude drug in whole 1g.
The Chinese medicine composition of described tablet form is applied to prevent and treat pig immunosuppressant syndrome, and its using method is oral: 2～4 of every days, be used in conjunction 10～15 days.
Embodiment 7
A Chinese medicine composition, the crude drug in whole proportioning of described Chinese medicine composition is: Radix Astragali 180g, Herba Epimedii 180g, Echinacea 135g, parts by weight are 180 parts of the Radixs Astragali, 180 parts of Herba Epimedii, 135 parts of Echinaceas.
The preparation method of above-mentioned Chinese medicine composition, comprises the following steps:
A, after being weighed by proportioning, each crude drug in whole pulverizes;
B, after being crossed to 60 mesh sieves, crude drug in whole is fully mixed with into powder.
The Chinese medicine composition of described powder type is applied to prevent and treat pig immunosuppressant syndrome, and its using method is mixed feeding: every 1kg feedstuff adds this Chinese medicine composition 5g, uses continuously 10～15 days.Because crude drug in whole proportioning and the preparation method of this embodiment are close with embodiment 1, thereby its prevention effect also should be close with embodiment 1.
In order to show the prevention effect of Chinese medicine composition of the present invention to porcine respiratory syndrome, medicine of the present invention is carried out to clinical observation on the therapeutic effect test, below by test, further set forth beneficial effect of the present invention.
In November, 2010, there are 480 piglets on Qingyuan City Germany and Britain pig farm, most of piglet occurs that feed intake declines or do not eat, cough, rhinorrhea, diarrhoea etc. cause piglet dead, after using medicine, death slightly subtracts, once but drug withdrawal starts again morbidity after several days, mortality rate increases, and is diagnosed as pig immunosuppressant syndrome.160 piglets selecting this pig farm, male and female pig all has, 40～50 ages in days, body weight is close, is divided into 8 groups, and every group of 20 piglet is for examination.
1. test grouping and administration: 1～6 group of test group, according to embodiment 1～6, be prepared medicine respectively, and respectively according to the using method medication of the embodiment of the present invention 1～6.Infecting matched group is to be infected but the piglet of not administration; Normal healthy controls group is the healthy piglet without catching; Matched group is only fed basic daily ration.Before test, weigh piglet, in test, weigh weekly piglet 1 time, calculate daily gain.Observation period is in 7 days after administration, observes body condition caval vein blood sampling, measures the every immune indexes of piglet.
2. index of assessment of curative effect:
(1) mortality rate=dead piglet ÷ test piglet sum * 100%;
(2) cure rate=piglet is cured number ÷ test piglet sum * 100%;
(3) the effective piglet of effective percentage=treat is counted ÷ test piglet sum * 100%;
(4) rate of body weight gain.The body weight of every pig when starting and during off-test, calculates the increment of every pig, then according to this, calculates average weight gain and the standard deviation of every group of test pig.Rate of body weight gain ÷ normal healthy controls group rate of body weight gain * 100% of the relative weight gain rate=each medication group or infected group, wherein normal healthy controls group is set as 100%;
(5) by detecting lymphocyte transformation test, the erythrocyte C of every group of test pig
3bthe average cell immunologic function of the test of receptor rosette rate and leukocytes phagocytic test evaluation piglet;
(6) by detecting every group of test pig EAC garland conversion ratio, evaluate the average humoral immune function of piglet.
3. data analysis and process: with SPSS 14.0 software data processings, wherein mortality rate, cure rate, effective percentage are all with Ka Shi check, and weightening finish adopts t check.
4. statistical results see the following form.
Table one medicine of the present invention suppresses disease curative effect statistics to piglet immunological
| Group time | Experimental animal head number/only | Mortality rate/% | Cure rate/% | Effective percentage/% | Average daily gain/g | Relative growth rate/% |
| Test group 1 | 20 | 5 | 70.00 | 85.00 | 129.7±1.4 | 83.3 |
| Test group 2 | 20 | 0 | 85.00 | 95.00 | 137.6±1.6 | 88.4 |
| Test group 3 | 20 | 0 | 90.00 | 100.00 | 146.3±1.4 | 94.0 |
| Test group 4 | 20 | 0 | 95.00 | 100.00 | 148.9±1.4 | 95.6 |
| Test group 5 | 20 | 0 | 90.00 | 95.00 | 138.1±1.4 | 88.7 |
| Test group 6 | 20 | 5 | 80.00 | 90.00 | 130.3±1.5 | 83.7 |
| Infect matched group | 20 | 30 | 20.00 | 25.00 | 44.3±1.3 | 28.4 |
| Normal healthy controls group | 20 | 0.00 | - | - | 155.7±1.5 | 100.00 |
The impact of table two the present invention on piglet immunological function
5. test result analysis:
(1 group is powder to 6 groups of medicine of the present invention, 2 groups is powder, 3 groups is granule, 4 groups is injection, 5 groups is oral liquid, 6 groups is tablet), test group 1～6 has significant difference (P<0.01) with infection matched group at mortality rate, cure rate, effective percentage, and 1～6 pair of pig immunosuppressant syndrome of test group all has significant curative effect; Test group 1～6 has difference in significant difference with infection matched group in the relative weight gain rate and extremely shows (P<0.01), but all lower than normal healthy controls group; Test group 1～6 with infect matched group and there is in significant difference difference extremely remarkable (P<0.0 1) improving cellular immunization and humoral immunization, and higher than normal healthy controls group.
Above content is in conjunction with concrete optimal way further description made for the present invention, should not assert that specific embodiment of the invention is confined to above explanation.For those skilled in the art, without departing from the inventive concept of the premise, can also make some simple deduction or replace, within the definite protection domain of the claim that all should be considered as being submitted to by the present invention.
Claims (9)
1. a Chinese medicine composition, is characterized in that, described Chinese medicine composition is prepared from by the crude drug in whole of following parts by weight proportioning: 50～200 parts of the Radixs Astragali, 30～200 parts of Herba Epimedii, 40～150 parts of Echinaceas.
2. Chinese medicine composition according to claim 1, is characterized in that, described Chinese medicine composition is prepared from by the crude drug in whole of following parts by weight proportioning: 70～200 parts of the Radixs Astragali, 40～180 parts of Herba Epimedii, 40～130 parts of Echinaceas.
3. Chinese medicine composition according to claim 1 and 2, is characterized in that: the dosage form of described Chinese medicine composition is any dosage form in powder, tablet, powder, granule, oral liquid, injection.
4. the preparation method of Chinese medicine composition according to claim 1 and 2, is characterized in that, comprises the following steps:
A, after being weighed by proportioning, each crude drug in whole pulverizes;
B, after being crossed to 24～100 mesh sieves, crude drug in whole is fully mixed with into powder.
5. the preparation method of Chinese medicine composition according to claim 1 and 2, is characterized in that, comprises the following steps:
A, each crude drug in whole weighed, pulverized by proportioning and mix after, with 30～60 minutes post-heating of water soaking crude drug in whole of 6～10 times of weight, to boiling, extract, extraction time is twice, boiling time is 1～3 hour;
B, filter after, merging filtrate the thick paste shape that relative density is 1.20～1.40 while being concentrated into 80 ℃;
C, the product of concentrated gained is added to conventional adjuvant, according to common process, be prepared into powder or tablet or granule.
6. the preparation method of Chinese medicine composition according to claim 5, is characterized in that: the described conventional adjuvant of step c comprises any one or more of starch, dextrin, sucrose, magnesium stearate.
7. the preparation method of Chinese medicine composition according to claim 1 and 2, is characterized in that, comprises the following steps:
A, each crude drug in whole weighed, pulverized by proportioning and mix after, with 30～60 minutes post-heating of water soaking crude drug in whole of 6～10 times of weight, to boiling, extract, extraction time is twice, boiling time is 1～3 hour;
B, filter after, merging filtrate is also concentrated into every 1ml and is equivalent to crude drug in whole 1～3g;
C, to adding 1.5～2.5 times of amount 75% ethanol in concentrated filtrate, stir, standing after precipitating leaching supernatant, reclaim ethanol, surplus liquid is concentrated into every 1ml and is equivalent to crude drug in whole 4～6g, is pulpous state;
D, according to common process, be prepared into oral liquid or injection.
8. the application of Chinese medicine composition according to claim 1 and 2 in the immunosuppressant syndrome medicament of preparation control pig.
9. the application of Chinese medicine composition according to claim 1 and 2 in the feedstuff of the immunosuppressant syndrome of preparation control pig.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104472880A (en) * | 2014-12-12 | 2015-04-01 | 四川乾坤生物科技有限公司 | Veterinary drug composition as well as preparation method and application thereof |
| CN105641031A (en) * | 2014-12-02 | 2016-06-08 | 北京大北农动物保健科技有限责任公司 | Compound pharmaceutical composition for strengthening body resistance and removing toxin, use and feed thereof |
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| CN104472880A (en) * | 2014-12-12 | 2015-04-01 | 四川乾坤生物科技有限公司 | Veterinary drug composition as well as preparation method and application thereof |
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