CN105168807A - Traditional Chinese medicine composition for treating porcine reproductive and respiratory syndrome and preparation method thereof - Google Patents

Abstract

The invention provides a traditional Chinese medicine composition for treating a porcine reproductive and respiratory syndrome and a preparation method thereof, and belongs to the field of veterinarian medications. The composition is mainly prepared from the following crude drugs in parts by weight: 10 to 25 parts of gorgon fruits, 8 to 10 parts of fructus forsythia, 5 to 9 parts of coptis chinensis, 7 to 13 parts of radix bupleuri, 10 to 20 parts of prepared liquorice roots, 9 to 16 parts of white paeony roots, 6 to 10 parts of garlic and 20 to 30 parts of a plant polysaccharide, wherein the plant polysaccharide is prepared by mixing an aloe polysaccharide, an astragalus membranaceus polysaccharide and a codonopsis pilosula polysaccharide at a mass ratio of (8-15) to (7-10) to (5-8). The medicine provided by the invention has the functions of clearing away internal heat and purging pathogenic fire, diminishing inflammation, and resisting bacteria and the like; the plant polysaccharide is added so that the immunity of pigs can be effectively improved and the morbidity of the porcine reproductive and respiratory syndrome is reduced; vitamin C can be added so that the composition has the advantages of promoting growth, preventing and controlling diseases and the like; the preparation method of the composition is simple, is safe and reliable, has no pollution and no residues and is easy to popularize.

Description

Chinese medicine composition for the treatment of pig blue-ear disease and preparation method thereof

Technical field

The invention belongs to veterinary medicament field, particularly a kind ofly treat the Chinese medicine composition of pig blue-ear disease and the preparation method of this Chinese medicine composition.

Background technology

Porcine reproductive and respiratory syndrome, is also called reproductive and respiratory syndrome, and be that a kind of pig caused by virus breathes and Reproduction Disorder, be the worldwide a kind of Infectious Diseases of pig industry, its high mortality and high infectious rate bring serious economic loss to pig farm.Pig is unique host of pig blue-ear disease, pig under various age, kind and various rearing conditions all can infect, but its susceptibility of the pig of all ages and classes has different, grower pigs and the metainfective symptom of fattening pig gentleer, and in-pig and the most susceptible of the piglet within 1 monthly age, symptom is then comparatively serious, and piglets case fatality rate can reach 80% ~ 100%.Ill pig and the malicious pig of band are the important sources of infection of primary disease.Major transmission path is that contact infection, air borne and seminal fluid are propagated, also by Placenta Hominis vertical transmission, the pig main manifestations suffering from pig blue-ear disease is the fertility performance decline of swinery, poor growth, the reproductive performance of sow group declines, Swinery immunity function reduction, other bacillary and viral diseases of easy secondary infection.Respiratory tract disease (as mycoplasma infection, contagious pleuropneumonia, streptococcicosis, the eperythrozoonosis) sickness rate of swinery rises.The pathogenic factor of pig blue-ear disease is mainly the destruction of the immunologic function of pig, causes infecting, and therefore, the effective way for the treatment of should be started with from the immunologic function of raising body to viral easy infection cell.

At present, the method for control pig blue-ear disease is more, mainly uses Western medicine, immunoprophylaxis, Chinese herbal medicine etc. to prevent and treat.Wherein, spreading unchecked that Western medicine uses brings great potential safety hazard to Pork food, and drug residue has become one of principal element affecting pork product safety.Immunoprophylaxis early stage results is better, but needs repeatedly to inoculate, troublesome poeration, and cost is higher, easily causes stress; And there is dispute in vaccination on Clinical practice, cannot large-scale promotion.Chinese herbal feed additive is current research direction.But current Chinese herbal feed additive exists medicine preparation more, and effect is undesirable, treatment is slow, the problems such as the course for the treatment of is long, and cure rate is low, and therefore, the curative effect improving the Chinese herbal feed additive for the treatment of pig blue-ear disease is still a great problem of this area.

Summary of the invention

The present invention solves in prior art to treat the not good problem of pig blue-ear disease Chinese herbal medicine additive therapeutic effect, provide a kind of Chinese medicine composition for the treatment of pig blue-ear disease and preparation method thereof, by the mode of feeding, said composition is applied to the prevention and control of pig blue-ear disease, reach the double effects of prevention and control reproductive and respiratory syndrome and enhancing immunity, reduce the sickness rate of pig blue-ear disease.

For achieving the above object, the present invention is achieved in that a kind of Chinese medicine composition for the treatment of pig blue-ear disease, said composition forms primarily of the crude drug of following weight portion: Semen Euryales 10 ~ 25 parts, Fructus Forsythiae 8 ~ 10 parts, Rhizoma Coptidis 5 ~ 9 parts, Radix Bupleuri 7 ~ 13 parts, Radix Glycyrrhizae Preparata 10 ~ 20 parts, the Radix Paeoniae Alba 9 ~ 16 parts, 6 ~ 10 parts, Bulbus Allii, vegetable polysaccharides 20 ~ 30 parts, and described vegetable polysaccharides mixes obtained by Aloe polysaccharide, astragalus polysaccharides and Radix Codonopsis polysaccharide according to mass ratio 8 ~ 15:7 ~ 10:5 ~ 8;

Described Aloe polysaccharide, astragalus polysaccharides and Radix Codonopsis polysaccharide adopt following extracting method respectively: cleaned up by raw material, after pulverizing, lixiviate, concentrating, in concentrated solution, add the mixed liquor of citric acid and ethanol, concentrated solution, citric acid and ethanol add according to the volume ratio of 2:1:1; Carry out microwave extraction after mixing, microwave irradiating time 30s ~ 50s, microwave power 700W, filter, with activated carbon decolorizing, after concentrated, namely obtain product.

The consumption of drug component of the present invention is that inventor draws through groping in a large number to sum up, each component has good therapeutic effect under following weight portion, Semen Euryales 15 parts, Fructus Forsythiae 9.5 parts, Rhizoma Coptidis 7.5 parts, Radix Bupleuri 10 parts, Radix Glycyrrhizae Preparata 12 parts, the Radix Paeoniae Alba 10.5 parts, 6.5 parts, Bulbus Allii, vegetable polysaccharides 28 parts.

Preferably, described vegetable polysaccharides mixes obtained by Aloe polysaccharide, astragalus polysaccharides and Radix Codonopsis polysaccharide according to mass ratio 13:8:7.

The each component of medicine of the present invention can also be: Semen Euryales 10 ~ 25 parts, Fructus Forsythiae 8 ~ 10 parts, Rhizoma Coptidis 5 ~ 9 parts, Radix Bupleuri 7 ~ 13 parts, Radix Glycyrrhizae Preparata 10 ~ 20 parts, the Radix Paeoniae Alba 9 ~ 16 parts, 6 ~ 10 parts, Bulbus Allii, vegetable polysaccharides 20 ~ 35 parts, vitamin C 3 ~ 9 parts.

Be more preferably Semen Euryales 15 parts, Fructus Forsythiae 9.5 parts, Rhizoma Coptidis 7.5 parts, Radix Bupleuri 10 parts, Radix Glycyrrhizae Preparata 12 parts, the Radix Paeoniae Alba 10.5 parts, 6.5 parts, Bulbus Allii, vegetable polysaccharides 28 parts, vitamin C 7.5 parts.

Medicament selection Semen Euryales of the present invention, Radix Bupleuri, Radix Glycyrrhizae Preparata, the Radix Paeoniae Alba, Bulbus Allii, Fructus Forsythiae, Rhizoma Coptidis and vegetable polysaccharides and vitamin C combine, and are made by these drug regimens each medicine effect produce synergism, thus effectively can treat pig blue-ear disease.Wherein Rhizoma Coptidis is used for the diseases such as retention of damp-heat in the interior, the intestines and stomach are damp and hot, vomiting, dysentery, for epidemic febrile disease high heat, thirsty agitation, bleeding due to blood-heat and pathopyretic ulcer etc., its main component berberine antagonism bacteriotoxin, reduction staphylococcus aureus coagulase, hemolysin are tired, reduce colibacillary virulence, itself and saikoside with the use of, have trivial solution heat effect to the caused heating such as typhoid fever, paratyphoid fever vaccine, escherichia coli liquid, fermented milk, yeast; And animal normal body temperature can be made to decline.Meanwhile, saikoside, has effect of Promote immunity function, improves special viral antibody titre, improves lymphocyte and turns core rate, improve skin delayed hypersensitivity reaction.Radix Glycyrrhizae Preparata contains the number of chemical compositions such as glycyrrhizin, enoxolone, Angelica Polysaccharide, has antiinflammatory and anti-allergy action, scalable body's immunity, antitumor and analgesic effect.The paeoniflorin contained in the Radix Paeoniae Alba has antibacterial, antipyretic, antiinflammatory, increase coronary flow, improves the effect of myocardial nutritional flow, blood vessel dilating, antagonism acute myocardial ischemia, anticoagulant, calmness, analgesia, spasmolytic, antiulcer, adjustment blood glucose.The high Aloe polysaccharide of purity, astragalus polysaccharides and Radix Codonopsis polysaccharide is obtained by microwave extraction, after mixing, join the main active that in Chinese medicinal components, it has can play one's part to the full after mixing with each taste monarch drug, there is the immunity that the effect of good scavenging activated oxygen, particularly Aloe polysaccharide and Radix Codonopsis polysaccharide can regulate body.

For reaching better curative effect, the present invention also selects Semen Euryales, Bulbus Allii, Fructus Forsythiae to combine, because Semen Euryales is put down, sweet in the mouth, puckery.Have the function of spleen reinforcing antidiarrheal, damp eliminating leukorrhagia stopping, its carbohydrate containing is very abundant, seldom fatty, is thus as easy as rolling off a logly absorbed.Bulbus Allii has removing toxic substances parasite killing, reducing swelling and alleviating pain, and antidiarrheal dysentery relieving, controls lung, anthelmintic, also has effect of warming the spleen warming the stomach in addition; Fructus Forsythiae heat clearing away, removing toxic substances, eliminating stagnation, detumescence.Control warm, erysipelas, macule, the effects such as carbuncle and ulcer toxic swelling.For obtaining optimum efficiency, the present invention also adds vitamin C on the basis of said medicine, can participate in the immunoreation of matrix.

The present invention also asks the preparation method of the Chinese medicine composition protecting above-mentioned treatment pig blue-ear disease, comprises the following steps:

(1) take Semen Euryales 10 ~ 25 parts, Radix Bupleuri 7 ~ 13 parts, Radix Glycyrrhizae Preparata 10 ~ 20 parts, the Radix Paeoniae Alba 9 ~ 16 parts, 6 ~ 10 parts, Bulbus Allii, Fructus Forsythiae 8 ~ 10 parts and Rhizoma Coptidis 5 ~ 9 parts, roguing, to dry;

(2) Semen Euryales, Radix Bupleuri, Radix Glycyrrhizae Preparata, the Radix Paeoniae Alba, Bulbus Allii are soaked 15 ~ 45 minutes, amount of water was advisable not have powder, decocted 2 ~ 4 hours, by medical filtration prior to 90 ~ 100 DEG C, decoct filtrate 1 ~ 1.5 hour in 40 ~ 50 DEG C again, centrifugal filtration obtains medicinal liquid I;

(3) Fructus Forsythiae, Rhizoma Coptidis are pulverized add 8 ~ 10 times of volumes water in 90 ~ 100 DEG C of boilings 1 ~ 3 hour, and then with mass concentration 90% ~ 95% alcohol reflux, ethanol recovery, by extracting liquid filtering, obtains medicinal liquid II stand-by;

(4) Aloe polysaccharide of extraction, astragalus polysaccharides and Radix Codonopsis polysaccharide are obtained vegetable polysaccharides according to proportioning 8 ~ 15:7 ~ 10:5 ~ 8 mix homogeneously;

(5) medicinal liquid I is mixed with medicinal liquid II, add vegetable polysaccharides 20 ~ 35 parts and vitamin C 3 ~ 9 parts, stir, in 50 ~ 60 DEG C of boilings 0.5 ~ 1 hour, in 45 ~ 50 DEG C of dryings, be ground into powder, obtain Chinese medicine composition.

The Chinese medicine composition for the treatment of pig blue-ear disease of the present invention can make the pharmaceutically acceptable dosage form of any one, spray, granule or decoction.

Medicine of the present invention can adopt the conventional method of Chinese medicine preparation to be prepared into any conventional oral preparations.Such as crude drug grind into powder mix homogeneously can be made powder to take after mixing it with water, can by raw material material decocting, then condensed water decocting liquid, but in order to make each raw material of this medicine play drug effect better, preferably carry out microwave extraction to the vegetable polysaccharides in raw material, Fructus Forsythiae, Rhizoma Coptidis carry out ethanol extraction.

Compositions of the present invention can add various customary adjuvant required when preparing different dosage form, as lubricant, binding agent etc. are prepared into any one common formulations with conventional method of Chinese medicinal, as spray, granule or decoction.

The invention has the beneficial effects as follows: medicine of the present invention has clearing away heat-fire, antiinflammatory, the function such as antibacterial, add the immunity that vegetable polysaccharides effectively can provide pig, reduce reproductive and respiratory syndrome sickness rate, the present invention can add vitamin C, growth promoting effects, the advantages such as prevention and control disease, and also said composition preparation method is simple, safe and reliable, pollution-free, noresidue, is easy to promote.

Detailed description of the invention

Set forth preparation method and the beneficial effect of medicine of the present invention by the following examples further.

Aloe polysaccharide, astragalus polysaccharides and Radix Codonopsis polysaccharide all adopt following extracting method: cleaned up by raw material, after pulverizing, lixiviate, concentrating, in concentrated solution, add the mixed liquor of citric acid and ethanol, concentrated solution, citric acid and ethanol add according to the volume ratio of 2:1:1; Carry out microwave extraction after mixing, microwave irradiating time 30s ~ 50s, microwave power 700W, filter, with activated carbon decolorizing, after concentrated, namely obtain product.

Embodiment 1

Semen Euryales 15 grams, Fructus Forsythiae 9.5 grams, Rhizoma Coptidis 7.5 grams, Radix Bupleuri 10 grams, Radix Glycyrrhizae Preparata 12 grams, the Radix Paeoniae Alba 10.5 grams, 6.5 grams, Bulbus Allii, vegetable polysaccharides 28 grams and vitamin C 7 grams.

Vegetable polysaccharides 28 grams mixes obtained by 13 grams of Aloe polysaccharides, 8 grams of astragalus polysaccharidess and 7 grams of Radix Codonopsis polysaccharides.

(1) take appropriate Semen Euryales, Radix Bupleuri, Radix Glycyrrhizae Preparata, the Radix Paeoniae Alba, Bulbus Allii, Fructus Forsythiae, Rhizoma Coptidis, roguing, to dry;

(2) Semen Euryales, Radix Bupleuri, Radix Glycyrrhizae Preparata, the Radix Paeoniae Alba, Bulbus Allii are soaked 15 ~ 45 minutes, amount of water was advisable not have powder, decocted 2 hours prior to 100 DEG C, and by medical filtration, then decoct filtrate 1.5 hours in 50 DEG C, centrifugal filtration obtains medicinal liquid I;

(3) Fructus Forsythiae, Rhizoma Coptidis are pulverized add 10 times of volumes water in 100 DEG C of boilings 1 hour, and then with mass concentration 95% alcohol reflux, ethanol recovery, by extracting liquid filtering, obtains medicinal liquid II stand-by;

(4) Aloe polysaccharide of extraction, astragalus polysaccharides and Radix Codonopsis polysaccharide are obtained vegetable polysaccharides according to proportioning mix homogeneously;

(5) medicinal liquid I is mixed with medicinal liquid II, add vegetable polysaccharides and vitamin C, stir, in 60 DEG C of boilings 1 hour, in 50 DEG C of dryings, be ground into powder, obtain Chinese medicine composition.

Gained Chinese medicine composition is added in deionized water and dissolves, adopt aerosol bottle packaging to obtain spray.

Embodiment 2

Semen Euryales 10 grams, Fructus Forsythiae 10 grams, Rhizoma Coptidis 9 grams, Radix Bupleuri 7 grams, Radix Glycyrrhizae Preparata 20 grams, the Radix Paeoniae Alba 9 grams, 6 grams, Bulbus Allii, vegetable polysaccharides 20 grams and vitamin C 3 grams.

Vegetable polysaccharides 20 grams mixes obtained by 8 grams of Aloe polysaccharides, 7 grams of astragalus polysaccharidess and 5 grams of Radix Codonopsis polysaccharides.

(1) take appropriate Semen Euryales, Radix Bupleuri, Radix Glycyrrhizae Preparata, the Radix Paeoniae Alba, Bulbus Allii, Fructus Forsythiae, Rhizoma Coptidis, roguing, to dry;

(2) Semen Euryales, Radix Bupleuri, Radix Glycyrrhizae Preparata, the Radix Paeoniae Alba, Bulbus Allii are soaked 15 ~ 45 minutes, amount of water was advisable not have powder, decocted 4 hours in 90 DEG C, and by medical filtration, then decoct filtrate 1 hour in 40 DEG C, centrifugal filtration obtains medicinal liquid I;

(3) Fructus Forsythiae, Rhizoma Coptidis are pulverized add 8 times of volumes water in 90 DEG C of boilings 2 hours, and then with mass concentration 95% alcohol reflux, ethanol recovery, by extracting liquid filtering, obtains medicinal liquid II stand-by;

(4) Aloe polysaccharide of extraction, astragalus polysaccharides and Radix Codonopsis polysaccharide are obtained vegetable polysaccharides according to proportioning mix homogeneously;

(5) medicinal liquid I is mixed with medicinal liquid II, add vegetable polysaccharides and vitamin C, stir, in 55 DEG C of boilings 1 hour, in 45 DEG C of dryings, be ground into powder, obtain Chinese medicine composition.

Gained Chinese medicine composition is ground into fine powder again, adds ethanol and make adhesive, add starch fill and, be pressed into granule.

Embodiment 3

Semen Euryales 25 grams, Fructus Forsythiae 9.5 grams, Rhizoma Coptidis 5 grams, Radix Bupleuri 12 grams, Radix Glycyrrhizae Preparata 10.5 grams, the Radix Paeoniae Alba 15 grams, 9 grams, Bulbus Allii, vegetable polysaccharides 30 grams and vitamin C 9 grams.

Vegetable polysaccharides 30 grams mixes obtained by 12 grams of Aloe polysaccharides, 10 grams of astragalus polysaccharidess and 8 grams of Radix Codonopsis polysaccharides.

(1) take appropriate Semen Euryales, Radix Bupleuri, Radix Glycyrrhizae Preparata, the Radix Paeoniae Alba, Bulbus Allii, Fructus Forsythiae, Rhizoma Coptidis, roguing, to dry;

(2) Semen Euryales, Radix Bupleuri, Radix Glycyrrhizae Preparata, the Radix Paeoniae Alba, Bulbus Allii are soaked 15 ~ 45 minutes, amount of water was advisable not have powder, decocted 3 hours in 100 DEG C, and by medical filtration, then decoct filtrate 1.5 hours in 50 DEG C, centrifugal filtration obtains medicinal liquid I;

(3) Fructus Forsythiae, Rhizoma Coptidis are pulverized add 10 times of volumes water in 100 DEG C of boilings 2 hours, and then with mass concentration 90% alcohol reflux, ethanol recovery, by extracting liquid filtering, obtains medicinal liquid II stand-by;

(4) Aloe polysaccharide of extraction, astragalus polysaccharides and Radix Codonopsis polysaccharide are obtained vegetable polysaccharides according to proportioning mix homogeneously;

(5) medicinal liquid I is mixed with medicinal liquid II, add vegetable polysaccharides and vitamin C, stir, in 50 DEG C of boilings 0.5 ~ 1 hour, in 45 DEG C of dryings, be ground into powder, obtain Chinese medicine composition.

Gained Chinese medicine composition is added in deionized water to dissolve to boil and make decoction.

Embodiment 4

Semen Euryales 20 grams, Fructus Forsythiae 8 grams, Rhizoma Coptidis 8.5 grams, Radix Bupleuri 13 grams, Radix Glycyrrhizae Preparata 10 grams, the Radix Paeoniae Alba 16 grams, 10 grams, Bulbus Allii and vegetable polysaccharides 26 grams.

Vegetable polysaccharides 33 grams mixes obtained by 15 grams of Aloe polysaccharides, 10 grams of astragalus polysaccharidess and 8 grams of Radix Codonopsis polysaccharides.

(1) take appropriate Semen Euryales, Radix Bupleuri, Radix Glycyrrhizae Preparata, the Radix Paeoniae Alba, Bulbus Allii, Fructus Forsythiae, Rhizoma Coptidis, roguing, to dry;

(2) Semen Euryales, Radix Bupleuri, Radix Glycyrrhizae Preparata, the Radix Paeoniae Alba, Bulbus Allii are soaked 15 ~ 45 minutes, amount of water was advisable not have powder, decocted 2 hours in 100 DEG C, and by medical filtration, then decoct filtrate 1.5 hours in 40 DEG C, centrifugal filtration obtains medicinal liquid I;

(3) Fructus Forsythiae, Rhizoma Coptidis are pulverized add 10 times of volumes water in 100 DEG C of boilings 3 hours, and then with mass concentration 90% alcohol reflux, ethanol recovery, by extracting liquid filtering, obtains medicinal liquid II stand-by;

(4) Aloe polysaccharide of extraction, astragalus polysaccharides and Radix Codonopsis polysaccharide are obtained vegetable polysaccharides according to proportioning mix homogeneously;

(5) medicinal liquid I is mixed with medicinal liquid II, add vegetable polysaccharides and vitamin C, stir, in 60 DEG C of boilings 1 hour, in 45 DEG C of dryings, be ground into powder, obtain Chinese medicine composition.

Gained Chinese medicine composition is added in deionized water and dissolves, be cooled to room temperature, adopt aerosol bottle packaging to obtain spray.

Test example 1:

1, test grouping: choosing suffers from piglet and each 100 of the Adult Pig of reproductive and respiratory syndrome, amount to 200, male and female dual-purpose, be divided into piglet group and Adult Pig group, be divided into four groups at random again by two groups, often organize 50, wherein two groups as a control group, one group is blank group of the quality water such as use, one group for using antibiotic treatment comparable group, other two groups is the treatment group using Chinese medicine composition different dosage form medicine of the present invention.

2, diagnostic criteria:

Body temperature is up to more than 40 DEG C, and dyspnea, sometimes in ventral breathing, loss of appetite or absolutely useless, diarrhoea, peels off, and stays alone or huddling together mutually, and by hair slightly disorderly, muscular tremor, unstable ataxia of walking, extremity abduction, posterior paralysis, gradually becomes thin, blepharoedema.Some piglet performance mouth and nose are very itched, and common nose dish, mouth end are on colony house friction wall hurdle, and nose has batter shape or watery secretion.

3, Therapeutic Method:

Spray often daily 3 ~ 5 times, each administration 10 ~ 30ml, is used in conjunction 14 days.Granule and decoction are mixed in feedstuff, conventionally feed, and each mixed volume is 0.1 ~ 0.2% of feedstuff gross mass, use 14 days continuously, within the 15th day, observe treatment situation.

4, efficacy assessment standard:

Effective: temperature of pig body is normal, without diarrhoea, breathe normal, without edema, spirit, appetite are normal;

Take a turn for the better: temperature of pig body is normal, without diarrhoea, have mild respiratory symptom, spirit, appetite are normal;

Invalid: not reach improvement standard or death.

5, therapeutic outcome

Table 1 Chinese medicine composition is to the therapeutical effect of pig blue-ear disease

Group	Dosage g/kg	Invalid head number	Survival rate	Cure rate
					Matched group	Deng water gaging	46	8％	0
Antibiotic group	10	41	17％	13％
					Medicinal herb spray group	16.5	12	76％	64％

Chinese medicine granules group

15

9

82％

74％

Note: compare * * * P<0.01 with matched group.

Test example 2

Chinese medicine composition prepared by the present invention is carried out clinical trial.

Clinical trial one:

In 37 1 monthly ages of pig farm, Dalian, piglet is doubtful ill, occur that heating, diarrhoea, dyspnea symptom are also with lassitude, appetite decline phenomenon, Traditional Chinese medicinal spray obtained by the present invention is treated, spray 5 every day, each 30ml, two weeks is a course for the treatment of, uses two courses for the treatment of continuously, and as shown in table 2 is observation of curative effect situation after the treatment of medicinal herb spray group.

Table 2

Clinical trial two:

60, pig farm, Dalian Adult Pig is doubtful ill, occur that heating, diarrhoea, dyspnea symptom are also with lassitude, appetite decline phenomenon, Chinese medicinal granule obtained by the present invention is treated, conventionally feed, each mixed volume is 0.1% of feedstuff gross mass, and as shown in table 3 is observation of curative effect situation after the treatment of Chinese medicine granules group.

Table 3

Clinical trial three:

In 42 1 monthly ages of pig farm, Dalian, piglet is doubtful ill, occur that heating, diarrhoea, dyspnea symptom are also with lassitude, appetite decline phenomenon, Chinese medicine decoction obtained by the present invention is treated, conventionally feed, each mixed volume is 0.2% of feedstuff gross mass, and as shown in table 4 is observation of curative effect situation after the treatment of Chinese medicine decoction group.

Table 4

Its reproductive and respiratory syndrome of piglet taking decoction of the present invention has obvious curative effect, and its effective percentage can reach 90.5%, and finds that this Chinese medicine plays somatotrophic effect to piglet.

The above; be only the present invention's preferably detailed description of the invention; but protection scope of the present invention is not limited thereto; anyly be familiar with those skilled in the art in the technical scope that the present invention discloses; be equal to according to technical scheme of the present invention and inventive concept thereof and replace or change, all should be encompassed within protection scope of the present invention.

Claims (6)

1. treat the Chinese medicine composition of pig blue-ear disease for one kind, it is characterized in that, said composition forms primarily of the crude drug of following weight portion: Semen Euryales 10 ~ 25 parts, Fructus Forsythiae 8 ~ 10 parts, Rhizoma Coptidis 5 ~ 9 parts, Radix Bupleuri 7 ~ 13 parts, Radix Glycyrrhizae Preparata 10 ~ 20 parts, the Radix Paeoniae Alba 9 ~ 16 parts, 6 ~ 10 parts, Bulbus Allii, vegetable polysaccharides 20 ~ 35 parts, and described vegetable polysaccharides mixes obtained by Aloe polysaccharide, astragalus polysaccharides and Radix Codonopsis polysaccharide according to mass ratio 8 ~ 15:7 ~ 10:5 ~ 8;

Described Aloe polysaccharide, astragalus polysaccharides and Radix Codonopsis polysaccharide adopt following extracting method respectively: cleaned up by raw material, after pulverizing, lixiviate, concentrating, in concentrated solution, add the mixed liquor of citric acid and ethanol, concentrated solution, citric acid and ethanol add according to the volume ratio of 2:1:1; Carry out microwave extraction after mixing, microwave irradiating time 30s ~ 50s, microwave power 700W, filter, with activated carbon decolorizing, after concentrated, namely obtain product.

2. the Chinese medicine composition for the treatment of pig blue-ear disease according to claim 1, it is characterized in that, in said composition, the consumption of each crude drug is: Semen Euryales 15 parts, Fructus Forsythiae 9.5 parts, Rhizoma Coptidis 7.5 parts, Radix Bupleuri 10 parts, Radix Glycyrrhizae Preparata 12 parts, the Radix Paeoniae Alba 10.5 parts, 6.5 parts, Bulbus Allii, vegetable polysaccharides 28 parts.

3. the Chinese medicine composition for the treatment of pig blue-ear disease according to claim 1, is characterized in that, described vegetable polysaccharides mixes obtained by Aloe polysaccharide, astragalus polysaccharides and Radix Codonopsis polysaccharide according to mass ratio 13:8:7.

4. the Chinese medicine composition for the treatment of pig blue-ear disease according to claim 1, is characterized in that, also has vitamin C 3 ~ 9 parts in crude drug.

5. a kind of preparation method for the treatment of the Chinese medicine composition of pig blue-ear disease as claimed in claim 1, is characterized in that, comprise the following steps:

(1) take Semen Euryales 10 ~ 25 parts, Radix Bupleuri 7 ~ 13 parts, Radix Glycyrrhizae Preparata 10 ~ 20 parts, the Radix Paeoniae Alba 9 ~ 16 parts, 6 ~ 10 parts, Bulbus Allii, Fructus Forsythiae 8 ~ 10 parts and Rhizoma Coptidis 5 ~ 9 parts, roguing, to dry;

(2) Semen Euryales, Radix Bupleuri, Radix Glycyrrhizae Preparata, the Radix Paeoniae Alba, Bulbus Allii are soaked 15 ~ 45 minutes, amount of water was advisable not have powder, decocted 2 ~ 4 hours, by medical filtration prior to 90 ~ 100 DEG C, decoct filtrate 1 ~ 1.5 hour in 40 ~ 50 DEG C again, centrifugal filtration obtains medicinal liquid I;

(3) Fructus Forsythiae, Rhizoma Coptidis are pulverized add 8 ~ 10 times of volumes water in 90 ~ 100 DEG C of boilings 1 ~ 3 hour, and then with mass concentration 90% ~ 95% alcohol reflux, ethanol recovery, by extracting liquid filtering, obtains medicinal liquid II stand-by;

(4) Aloe polysaccharide of extraction, astragalus polysaccharides and Radix Codonopsis polysaccharide are obtained vegetable polysaccharides according to proportioning 8 ~ 15:7 ~ 10:5 ~ 8 mix homogeneously;

(5) medicinal liquid I is mixed with medicinal liquid II, add vegetable polysaccharides 20 ~ 35 parts and vitamin C 3 ~ 9 parts, stir, in 50 ~ 60 DEG C of boilings 0.5 ~ 1 hour, in 45 ~ 50 DEG C of dryings, be ground into powder, obtain Chinese medicine composition.

6. the preparation method of the Chinese medicine composition for the treatment of pig blue-ear disease according to claim 1, is characterized in that, can make the pharmaceutically acceptable dosage form of any one, spray, granule or decoction.