CN113730481B

CN113730481B - Traditional Chinese medicine composition for preventing and treating canine parvovirus enteritis, suppository and preparation method

Info

Publication number: CN113730481B
Application number: CN202110975590.XA
Authority: CN
Inventors: 李云波; 杜西翠
Original assignee: Hebei Greenland Animal Pharmaceutical Co ltd
Current assignee: Hebei Greenland Animal Pharmaceutical Co ltd
Priority date: 2021-08-24
Filing date: 2021-08-24
Publication date: 2022-12-20
Anticipated expiration: 2041-08-24
Also published as: CN113730481A

Abstract

The invention relates to the technical field of veterinary medicines, and particularly relates to a traditional Chinese medicine composition for preventing and treating canine parvovirus enteritis, a suppository and a preparation method. The traditional Chinese medicine composition is prepared from raw materials including coptis chinensis, scutellaria baicalensis, rheum officinale, angelica sinensis, radix paeoniae alba, sophora japonica, herba patriniae, cacumen biotae, fructus evodiae and pericarpium granati, is used for treating canine parvovirus enteritis through rectal administration, has the effects of clearing heat, promoting blood circulation, stopping dysentery and recovering yin, can take effect quickly, is high in cure rate, does not have side effects, and is also suitable for puppies. The suppository is more convenient for clinical use after being prepared into the suppository.

Description

Traditional Chinese medicine composition for preventing and treating canine parvovirus enteritis, suppository and preparation method

Technical Field

The invention relates to the technical field of traditional Chinese veterinary medicines, and in particular relates to a traditional Chinese medicine composition for preventing and treating canine parvovirus enteritis, a suppository and a preparation method.

Background

The canine parvovirus disease is an acute infectious disease which is caused by infection of dogs by Canine Parvovirus (CPV) and is mainly characterized by hemorrhagic enteritis, is mainly characterized by severe vomiting, diarrhea and obvious reduction of leucocytes, is one of common clinical diseases, has high mortality, high development speed and strong infectivity, and is one of the most important virulent infectious diseases damaging dogs.

CPV primarily infects dogs, occurring in dogs of various ages, and mostly occurs in puppies of 3 to 4 months of age. The canine parvovirus enteritis generally has a incubation period of 7-14 days, the initial body temperature of the canine parvovirus enteritis rises to 40 ℃, the appetite is exhausted, dehydration symptoms are accompanied, and then vomit and diarrhea occur, the excrement is thin and stinky and is yellow or gray yellow, the excrement contains a large amount of mucus and mucous membrane, and the excrement is tomato juice-like, has blood filaments and is stinky. Late stage of urinary incontinence, sunken eyes, slow reaction, yellow tongue coating and bad breath. As the virus antigen drifts, the virus can infect animals such as cats, bears, raccoon dogs and the like.

The disease is acute in onset and short in course, if the disease is not treated in time, the dog can die, but no specific therapy is available at present, mainly dehydration is corrected according to symptomatic treatment of the disease, and hyper-immune serum therapy, antibiotic therapy and the like are assisted to prevent secondary infection. On one hand, the existing administration mode is infusion treatment, and the coordination degree of the sick dogs is poor; on the other hand, antibiotics are easy to cause adverse reactions of different degrees, especially for puppies, the use of antibiotics is easy to cause body damage, for example, quinolones can cause cartilage dysplasia of puppies, so that the antibiotics need to be used with caution. In addition, the cured dogs still carry viruses, and the viruses are discharged out of the body along with excrement, urine and vomit to pollute the surrounding environment, so that other dogs are infected with diseases, and the diseases are difficult to completely eliminate when the diseases occur in the dog groups.

Disclosure of Invention

Aiming at the technical problems in the prior art, the invention provides a traditional Chinese medicine composition for preventing and treating canine parvovirus enteritis, a suppository and a preparation method. The traditional Chinese medicine composition can exert curative effect by rectal administration, is convenient to administer, and has the advantages of quick response, high cure rate and good prevention effect.

In order to solve the above technical problems, a first aspect of the embodiments of the present invention provides a traditional Chinese medicine composition for preventing and treating canine parvoviral enteritis, comprising the following raw materials: coptis root, scutellaria baicalensis, rheum officinale, angelica sinensis, white paeony root, at least one of lotus plumule, sophora flower and gardenia, at least one of rheum officinale, picria fel-terrae lour, patrinia, chinese violet and cacumen biotae, at least one of perilla stem, fructus evodiae and costus root, and at least one of halloysite, gallnut and pomegranate rind.

The Chinese medicinal composition is used for treating canine parvovirus enteritis by rectal administration, has the effects of clearing heat, promoting blood circulation, stopping dysentery and recovering yin, can take effect quickly, has no side effect, and is also suitable for puppies.

Coptis root is bitter and cold, clears heat and dries dampness, and purges fire and detoxifies;

the scutellaria is bitter and cold, clears heat and dries dampness, purges fire and detoxifies;

rhubarb, radix et rhizoma Rhei, being bitter and cold, purges heat and purges intestines, cools blood and detoxifies, breaks accumulation and removes stasis;

the angelica sinensis is sweet, pungent and warm, and has the effects of enriching and nourishing blood, promoting blood circulation to relieve pain, moistening dryness and relaxing bowels;

white peony root, radix Paeoniae alba is bitter, sour and slightly cold, calms the liver, relieves pain, nourishes blood and astringes yin;

the lotus plumule is bitter and cold, clears heart fire, calms liver fire, communicates heart and kidney, and astringes essence to stop bleeding;

the sophora flower is bitter and slightly cold, and has the effects of cooling blood, stopping bleeding, clearing liver and purging fire;

fructus Gardeniae is bitter and cold, has effects in purging pathogenic fire, relieving restlessness, clearing away heat, promoting urination, cooling blood, and removing toxic substance;

the rhubarb is pungent, bitter and cool, has the functions of clearing away heat and toxic material, cooling blood and stopping bleeding, removing blood stasis and detumescence, and killing parasites;

the picria felterrae lour is bitter and cool, and has the effects of clearing away heat and toxic materials, relieving swelling and pain;

herba Patriniae is pungent, bitter and cool, and has effects of clearing away heat and toxic materials, removing blood stasis, relieving pain, relieving swelling, and expelling pus;

the Chinese violet is bitter, pungent and cold, and has the effects of clearing away heat and toxic materials, cooling blood and relieving swelling;

the arborvitae tops are bitter, astringent and cold, cool blood and stop bleeding;

the perilla stem is pungent and warm, regulates qi, relieves epigastric distention, and relieves stagnation and pain;

the evodia rutaecarpa is pungent, bitter and hot, warms the middle-jiao to relieve pain, and regulates qi to arrest vomiting;

the costustoot is pungent, bitter and warm, warms the middle warmer, promotes qi circulation and relieves pain;

halloysitum rubrum is sweet, sour, astringent and warm, and has effects of astringing intestine, stopping bleeding, promoting granulation, and healing sore;

galla chinensis is sour, astringent and cold, and has effects of lowering fire, stopping bleeding, relieving diarrhea with astringents, eliminating dampness and healing sore;

pomegranate rind is sour, astringent and warm, astringes intestines to check diarrhea, stops bleeding and expels parasites.

The medicines are compatible, and the obtained composition has the effects of clearing heat, activating blood, stopping dysentery and collecting yin, and is very suitable for treating canine parvovirus enteritis.

Preferably, the traditional Chinese medicine composition can comprise the following raw materials in parts by weight: 27 to 36 parts of coptis chinensis, 20 to 29 parts of scutellaria baicalensis, 30 to 50 parts of rheum officinale, 30 to 50 parts of angelica, 28 to 37 parts of white paeony root, 10 to 15 parts of lotus plumule, 8 to 17 parts of gardenia, 10 to 17 parts of rheum officinale, 12 to 18 parts of herba patriniae, 40 to 60 parts of perilla stem and 20 to 30 parts of gallnut. In the composition, the coptis root and the scutellaria root are used as monarch drugs for clearing heat and drying dampness, purging fire and detoxifying, and the angelica and the white paeony root are used for enriching the blood and nourishing the blood, astringing yin and relieving pain; rhubarb, radix et rhizoma Rhei purges heat and purges intestines, eliminates dampness-heat accumulation stagnation in stomach and intestine, is matched with lotus plumule, cape jasmine fruit, rhubarb and herba Patriniae to clear heat and calm the liver, cool blood and stop bleeding, and is used as a ministerial drug; perilla stem, fructus Perillae, as an adjuvant drug, regulates qi-flowing for relieving pain, galla chinensis, astringes intestine to stop diarrhea, promotes granulation and heals wound. The further preferable mixture ratio of the raw materials is as follows: 30 to 33 parts of coptis chinensis, 23 to 26 parts of scutellaria baicalensis, 35 to 45 parts of rheum officinale, 35 to 45 parts of angelica sinensis, 31 to 35 parts of radix paeoniae alba, 11 to 13 parts of lotus plumule, 10 to 14 parts of gardenia, 12 to 15 parts of rheum officinale, 14 to 17 parts of herba patriniae, 45 to 55 parts of perilla stem and 22 to 27 parts of gallnut. The further preferable mixture ratio is as follows: 31 parts of coptis chinensis, 25 parts of scutellaria baicalensis, 40 parts of rheum officinale, 40 parts of angelica sinensis, 33 parts of radix paeoniae alba, 12 parts of lotus plumule, 13 parts of gardenia, 13 parts of rheum officinale, 16 parts of herba patriniae, 50 parts of perilla stem and 25 parts of gallnut.

Preferably, the traditional Chinese medicine composition can also comprise the following raw materials in parts by weight: 27 to 36 parts of coptis chinensis, 20 to 29 parts of scutellaria baicalensis, 30 to 50 parts of rheum officinale, 30 to 50 parts of angelica, 28 to 37 parts of white paeony root, 25 to 32 parts of sophora flower, 15 to 23 parts of picria felterrae lour, 5 to 12 parts of Chinese violet, 15 to 22 parts of cacumen biotae, 30 to 42 parts of costustoot and 18 to 22 parts of pomegranate bark. In the composition, the coptis root and the scutellaria root are used as monarch drugs for clearing heat and drying dampness, purging fire and detoxifying, and the angelica and the white paeony root are used for enriching the blood and nourishing the blood, astringing yin and relieving pain; rhubarb, radix et rhizoma Rhei purges heat and purges intestines, eliminates dampness-heat accumulation in stomach and intestine, is matched with flos Sophorae Immaturus, picria fel-terrae lour, herba Violae and cacumen Platycladi to cool blood and stop bleeding, clear heat and remove toxicity, and is used as a ministerial drug; mu Xiang warms the middle-jiao to alleviate pain, gu Pi astringes intestine to stop diarrhea and prevents collapse, so it is used as adjuvant drug. The further preferable mixture ratio of the raw materials is as follows: 30 to 33 parts of coptis chinensis, 23 to 26 parts of scutellaria baicalensis, 35 to 45 parts of rheum officinale, 35 to 45 parts of angelica, 31 to 35 parts of radix paeoniae alba, 27 to 30 parts of sophora flower, 17 to 21 parts of picria felterrae lour, 7 to 10 parts of Chinese violet, 17 to 20 parts of cacumen biotae, 34 to 39 parts of elecampane and 19 to 21 parts of pomegranate rind. The further preferable mixture ratio is as follows: 32 parts of coptis chinensis, 24 parts of scutellaria baicalensis, 40 parts of rheum officinale, 40 parts of angelica sinensis, 33 parts of radix paeoniae alba, 29 parts of sophora flower, 19 parts of picria felterrae lour, 8 parts of Chinese violet, 18 parts of cacumen biotae, 36 parts of elecampane and 20 parts of pomegranate bark.

Preferably, the traditional Chinese medicine composition can also comprise the following raw materials in parts by weight: 27 to 36 parts of coptis chinensis, 20 to 29 parts of scutellaria baicalensis, 30 to 50 parts of rheum officinale, 30 to 50 parts of Chinese angelica, 28 to 37 parts of white paeony root, 25 to 32 parts of sophora japonica, 23 to 32 parts of herba patriniae, 16 to 23 parts of cacumen platycladi, 30 to 38 parts of fructus evodiae and 18 to 22 parts of pericarpium granati. In the composition, the coptis and the scutellaria clear heat and dry dampness, purge fire and remove toxicity, and the angelica and the white paeony root enrich the blood and nourish the blood, astringe yin and relieve pain are monarch drugs; rhubarb, radix et rhizoma Rhei purges heat and intestines, eliminates dampness-heat accumulation in stomach and intestines, is matched with flos Sophorae Immaturus, herba Patriniae and cacumen Platycladi to cool blood and stop bleeding, purge fire and remove toxicity, and is used as a ministerial drug; evodia rutaecarpa, fructus evodiae, as an adjuvant drug, has the effects of warming the middle-jiao to alleviate pain, regulating qi to arrest vomiting, and pomegranate bark, pericarpium granati, as an astringent, relieving diarrhea and preventing collapse. The further preferable mixture ratio of the raw materials is as follows: 30 to 34 parts of coptis chinensis, 22 to 26 parts of scutellaria baicalensis, 35 to 45 parts of rheum officinale, 35 to 45 parts of angelica, 30 to 34 parts of white paeony root, 26 to 30 parts of sophora flower, 26 to 30 parts of herba patriniae, 18 to 22 parts of cacumen biotae, 32 to 36 parts of fructus evodiae and 18 to 22 parts of pomegranate bark. The more preferable mixture ratio is as follows: 32 parts of coptis chinensis, 24 parts of scutellaria baicalensis, 40 parts of rheum officinale, 40 parts of angelica sinensis, 32 parts of radix paeoniae alba, 28 parts of sophora flower, 28 parts of patrinia, 20 parts of cacumen biotae, 34 parts of fructus evodiae and 20 parts of pomegranate rind.

Preferably, the traditional Chinese medicine composition can also comprise the following raw materials in parts by weight: 27 to 36 parts of coptis chinensis, 20 to 29 parts of scutellaria baicalensis, 30 to 50 parts of rheum officinale, 30 to 50 parts of angelica, 28 to 37 parts of radix paeoniae alba, 16 to 22 parts of sophora japonica, 5 to 13 parts of gardenia, 12 to 16 parts of rheum officinale, 25 to 32 parts of philippine violet herb, 15 to 23 parts of evodia rutaecarpa, 15 to 23 parts of costustoot, 12 to 19 parts of red halloysite and 10 to 18 parts of pomegranate bark. In the composition, the coptis root and the scutellaria root are used as monarch drugs for clearing heat and drying dampness, purging fire and detoxifying, and the angelica and the white paeony root are used for enriching the blood and nourishing the blood, astringing yin and relieving pain; rhubarb, radix et rhizoma Rhei purges heat and purges intestines, eliminates the stagnation of damp-heat in the stomach and intestine, is matched with flos sophorae, fructus gardeniae, rhubarb root and herba violae to clear heat and calm the liver, cool blood and stop bleeding, and relieve swelling and detoxify, and is used as a ministerial drug; evodia rutaecarpa and aucklandia root, radix aucklandiae, as adjuvant drugs, have the effects of warming the middle-jiao to alleviate pain, regulating the flow of qi to arrest vomiting, halloysitum rubrum and pomegranate rind, relieving diarrhea with astringents, promoting granulation and healing wound. The further preferable mixture ratio of the raw materials is as follows: 29 to 34 parts of coptis chinensis, 25 to 27 parts of scutellaria baicalensis, 35 to 45 parts of rheum officinale, 35 to 45 parts of Chinese angelica, 30 to 34 parts of white paeony root, 18 to 20 parts of sophora japonica, 7 to 11 parts of gardenia, 13 to 15 parts of rheum officinale, 28 to 30 parts of Chinese violet, 17 to 20 parts of evodia rutaecarpa, 17 to 20 parts of costustoot, 15 to 17 parts of red halloysite and 12 to 16 parts of pomegranate bark. The further preferable mixture ratio is as follows: 32 parts of coptis chinensis, 26 parts of scutellaria baicalensis, 40 parts of rheum officinale, 40 parts of angelica sinensis, 32 parts of radix paeoniae alba, 19 parts of sophora flower, 9 parts of gardenia, 14 parts of rheum officinale, 29 parts of Chinese violet, 18 parts of fructus evodiae, 18 parts of elecampane, 16 parts of red halloysite and 14 parts of pomegranate bark.

In a second aspect, the embodiment of the invention also provides a suppository for preventing and treating canine parvovirus enteritis, and the active ingredients of the suppository comprise the traditional Chinese medicine composition for preventing and treating canine parvovirus enteritis. The traditional Chinese medicine composition is prepared into suppository, so that rectal administration is more convenient.

In a third aspect, the embodiment of the invention also provides a preparation method of the suppository for preventing and treating canine parvoviral enteritis, which specifically comprises the following operations:

weighing the raw materials in the traditional Chinese medicine composition according to the weight part ratio, decocting the raw materials in water for extraction, concentrating the obtained extracting solution, and drying the extracting solution to prepare traditional Chinese medicine extract powder; melting semisynthetic fatty glyceride, adding the Chinese medicinal extract powder, mixing, and molding.

Wherein the operation of water decoction and extraction is preferably 1 to 3 times, 1.5 to 2h each time. The semisynthetic fatty glyceride can be selected from common semisynthetic fatty glyceride in suppository such as coconut oil ester, palm oil ester, pungent litse fruit oil ester or propylene glycol stearate.

In a fourth aspect, the embodiment of the invention also provides another preparation method of the suppository for preventing and treating canine parvoviral enteritis, which specifically comprises the following operations:

weighing the raw materials in the traditional Chinese medicine composition according to the weight part ratio, wherein the angelica is the Chinese angelica stir-fried with charcoal;

reflux-extracting the coptis chinensis by using 0.5% -1.0% v/v sulfuric acid aqueous solution to obtain an extracting solution and coptis chinensis residues, adjusting the pH of the extracting solution to 1-1.5 by using concentrated hydrochloric acid, filtering, washing the obtained filter cake to be neutral by using water, and drying to obtain a first coptis chinensis extract; placing the coptis chinensis residue in water, performing enzymolysis by using cellulase, filtering, concentrating the filtrate, and drying to obtain a second extract of coptis chinensis;

reflux-extracting the radix Angelicae sinensis with ethanol water solution, filtering, concentrating the filtrate, and drying to obtain radix Angelicae sinensis extract;

ultrasonically extracting the white paeony root with an ethanol water solution to obtain white paeony root ethanol extract and white paeony root residues, extracting the white paeony root residues with water under reflux to obtain white paeony root water extract, respectively concentrating and drying the white paeony root ethanol extract and the white paeony root water extract, and mixing to obtain a white paeony root extract;

reflux-extracting Scutellariae radix with acetic acid water solution to obtain Scutellariae radix acid extractive solution and Scutellariae radix residue, reflux-extracting Scutellariae radix residue with ethanol water solution to obtain Scutellariae radix ethanol extractive solution, concentrating and drying the Scutellariae radix acid extractive solution and Scutellariae radix ethanol extractive solution respectively, and mixing to obtain Scutellariae radix extract;

reflux-extracting the rhubarb with a sodium bicarbonate aqueous solution with the pH value of 7.5-8.0, concentrating and drying the obtained extract to obtain a rhubarb extract;

mixing the rest materials, extracting with water under reflux, concentrating the extractive solution, and drying to obtain mixed extract;

mixing the first extract of Coptidis rhizoma, the second extract of Coptidis rhizoma, the extract of Angelicae Gigantis radix, paeoniae alba, scutellariae radix, and radix et rhizoma Rhei with the mixed extract to obtain Chinese medicinal extract powder; melting semisynthetic fatty glyceride, adding the Chinese medicinal extract powder, mixing, and molding.

Preferably, the mass of the cellulase is 0.5% -1.5% of the mass of the coptis chinensis residue, the enzymolysis temperature is 35-45 ℃, and the enzymolysis time is 3-5h.

Preferably, the aqueous ethanol solution from which the angelica extract is prepared has a concentration of 50% to 60% v/v, the aqueous ethanol solution containing 1.5% to 3.5% m/m sodium chloride.

Preferably, the concentration of the aqueous ethanol solution for preparing the white peony root extract is 45% -60%, v/v, and the parameters of ultrasonic extraction are 45-50Hz, 240-250W.

Preferably, the concentration of said aqueous acetic acid solution for preparing said Scutellariae radix extract is 0.15% to 0.25% v/v, the concentration of said aqueous ethanol solution is 40% to 60% v/v.

The traditional Chinese medicine raw materials are extracted according to the method and then are prepared into suppository, so that the curative effect can be further improved, and the treatment effect on puppies is particularly remarkable.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.

The canine parvoviral enteritis has acute onset and short course of disease, is mainly treated according to the illness condition, mainly corrects dehydration, is assisted by hyperimmune serotherapy, antibiotic treatment and the like, prevents secondary infection, is mostly treated by infusion, and has poor matching degree for sick dogs. Moreover, antibiotics can cause adverse reactions of different degrees, and particularly, the antibiotics are more obvious in body damage caused by puppies. The cured sick dogs also carry viruses continuously, and the viruses infect other dogs through excrement, urine, vomit and the like, so that the dogs are difficult to completely eliminate in a dog group once the dogs are infected with the viruses.

The traditional Chinese medicine considers that the disease is caused by damp-heat and steaming, heat-toxin stagnation, qi-blood stagnation, toxin stagnation in intestines, stagnation and blood stasis, and heat injury to blood system. Aiming at the adverse reaction caused by antibiotics, the traditional Chinese medicine is used for treating canine parvovirus enteritis in the prior art. The traditional Chinese medicine has the advantages of no drug residue, no hormone, no drug resistance, wide drug sources, local materials, low price, stable curative effect, small toxic and side effects and the like, and the traditional Chinese medicine formula with reasonable compatibility can resist bacteria, diminish inflammation, resist viruses and expel and kill parasites, and vitamins, mineral substances, trace elements, specific natural compounds and the like contained in the traditional Chinese medicine formula can provide nutrition for sick animals and improve the health condition of organisms. However, most of the treatments with traditional Chinese medicines have slow effect and uncertain curative effect, and most of the traditional Chinese medicines have poor smell, so that the administration is difficult and the intake can not be ensured.

In order to solve the problems of inconvenient administration, difficult administration and slow response, the embodiment of the invention provides a traditional Chinese medicine composition for preventing and treating canine parvoviral enteritis, which comprises the following raw materials: coptis root, scutellaria baicalensis, rheum officinale, angelica sinensis, white paeony root, at least one of lotus plumule, sophora flower and gardenia, at least one of rheum officinale, picria fel-terrae lour, patrinia, chinese violet and cacumen biotae, at least one of perilla stem, fructus evodiae and costus root, and at least one of halloysite, gallnut and pomegranate rind. The traditional Chinese medicine composition is a traditional Chinese medicine formula which can exert curative effect through rectal administration, has the effects of clearing heat, activating blood circulation, stopping dysentery and collecting yin, is quick in effect taking, has no side effect, is very suitable for treating canine parvovirus enteritis, has excellent treatment and prevention effects on puppies, and has no adverse reaction.

The embodiment of the application provides a combination with an optimal proportion, which comprises the following raw materials in parts by weight: 27 to 36 parts of coptis chinensis, 20 to 29 parts of scutellaria baicalensis, 30 to 50 parts of rheum officinale, 30 to 50 parts of angelica, 28 to 37 parts of white paeony root, 10 to 15 parts of lotus plumule, 8 to 17 parts of gardenia, 10 to 17 parts of rheum officinale, 12 to 18 parts of herba patriniae, 40 to 60 parts of perilla stem and 20 to 30 parts of Chinese gall. The method comprises the following steps: the coptis root and the scutellaria root are used as monarch drugs for clearing heat, drying dampness, purging fire and removing toxin, and the angelica and the white paeony root are used for enriching the blood, nourishing the blood, astringing yin and relieving pain; rhubarb, radix et rhizoma Rhei purges heat and purges intestines, eliminates the stagnation of damp-heat in the stomach and intestine, is matched with lotus plumule, cape jasmine fruit, rhubarb and herba patriniae to clear heat and calm the liver, cool blood and stop bleeding, and is used as a ministerial drug; perilla stem is used as adjuvant drug for regulating qi-flowing and relieving pain, and Galla chinensis is used for astringing intestine to stop diarrhea, promoting granulation and healing sore. In the embodiment of the application, the further preferable mixture ratio of the raw materials is as follows: 30 to 33 parts of coptis chinensis, 23 to 26 parts of scutellaria baicalensis, 35 to 45 parts of rheum officinale, 35 to 45 parts of angelica sinensis, 31 to 35 parts of radix paeoniae alba, 11 to 13 parts of lotus plumule, 10 to 14 parts of gardenia, 12 to 15 parts of rheum officinale, 14 to 17 parts of herba patriniae, 45 to 55 parts of perilla stem and 22 to 27 parts of gallnut. The more preferable mixture ratio is: 31 parts of coptis chinensis, 25 parts of scutellaria baicalensis, 40 parts of rheum officinale, 40 parts of angelica sinensis, 33 parts of radix paeoniae alba, 12 parts of lotus plumule, 13 parts of gardenia, 13 parts of rheum officinale, 16 parts of herba patriniae, 50 parts of perilla stem and 25 parts of gallnut.

The embodiment of the application provides another combination with a preferable mixture ratio, which comprises the following raw materials in parts by weight: 27 to 36 parts of coptis chinensis, 20 to 29 parts of scutellaria baicalensis, 30 to 50 parts of rheum officinale, 30 to 50 parts of Chinese angelica, 28 to 37 parts of white paeony root, 25 to 32 parts of sophora japonica, 15 to 23 parts of picria felterrae lour, 5 to 12 parts of Chinese violet, 15 to 22 parts of cacumen biotae, 30 to 42 parts of elecampane and 18 to 22 parts of pomegranate bark. The method comprises the following steps: the coptis and the scutellaria clear heat and dry dampness, purge fire and remove toxin, and the angelica and the white paeony root enrich blood and nourish blood, astringe yin and relieve pain, which are monarch drugs; rhubarb, radix et rhizoma Rhei purges heat and purges intestines, eliminates the stagnation of damp-heat in the stomach and intestine, is matched with flos sophorae, picria felterrae lour, herba violae and cacumen biotae to cool blood and stop bleeding, clear heat and remove toxicity, and is used as a ministerial medicine; mu Xiang warms the middle-jiao to alleviate pain, and Shi Gu Pi astringes intestines to check diarrhea and prevent collapse is used as adjuvant drug. In the embodiment of the application, the further preferable mixture ratio of the raw materials is as follows: 30 to 33 parts of coptis chinensis, 23 to 26 parts of scutellaria baicalensis, 35 to 45 parts of rheum officinale, 35 to 45 parts of Chinese angelica, 31 to 35 parts of white paeony root, 27 to 30 parts of sophora japonica, 17 to 21 parts of picria felterrae lour, 7 to 10 parts of Chinese violet, 17 to 20 parts of cacumen biotae, 34 to 39 parts of elecampane and 19 to 21 parts of pomegranate rind. The more preferable mixture ratio is as follows: 32 parts of coptis root, 24 parts of scutellaria baicalensis, 40 parts of rhubarb, 40 parts of angelica, 33 parts of white peony root, 29 parts of sophora flower, 19 parts of picria felterrae lour, 8 parts of Chinese violet, 18 parts of cacumen biotae, 36 parts of elecampane and 20 parts of pomegranate bark.

The embodiment of the application provides another combination with a preferable mixture ratio, which comprises the following raw materials in parts by weight: 27 to 36 parts of coptis chinensis, 20 to 29 parts of scutellaria baicalensis, 30 to 50 parts of rheum officinale, 30 to 50 parts of angelica, 28 to 37 parts of radix paeoniae alba, 25 to 32 parts of sophora flower, 23 to 32 parts of herba patriniae, 16 to 23 parts of cacumen biotae, 30 to 38 parts of fructus evodiae and 18 to 22 parts of pomegranate bark. The method comprises the following steps: the coptis root and the scutellaria root are used as main medicines for clearing heat and drying dampness, purging fire and removing toxin, and the angelica and the white paeony root are used for enriching the blood and nourishing the blood, astringing yin and relieving pain; rhubarb, radix et rhizoma Rhei purges heat and purges intestines, eliminates the stagnation of damp-heat in the stomach and intestine, is matched with flos sophorae, herba patriniae and cacumen biotae to cool blood and stop bleeding, purge fire and detoxify as assistant drugs; evodia rutaecarpa and pomegranate rind act as adjuvant drugs for warming the middle-jiao to alleviate pain, regulating qi to arrest vomiting, astringing intestines to arrest diarrhea and preventing collapse. In the embodiment of the application, the further preferable mixture ratio of the raw materials is as follows: 30 to 34 parts of coptis chinensis, 22 to 26 parts of scutellaria baicalensis, 35 to 45 parts of rheum officinale, 35 to 45 parts of Chinese angelica, 30 to 34 parts of white paeony root, 26 to 30 parts of sophora japonica, 26 to 30 parts of herba patriniae, 18 to 22 parts of cacumen platycladi, 32 to 36 parts of fructus evodiae and 18 to 22 parts of pericarpium granati. The more preferable mixture ratio is: 32 parts of coptis chinensis, 24 parts of scutellaria baicalensis, 40 parts of rheum officinale, 40 parts of angelica sinensis, 32 parts of radix paeoniae alba, 28 parts of sophora flower, 28 parts of herba patriniae, 20 parts of cacumen biotae, 34 parts of fructus evodiae and 20 parts of pomegranate rind.

The embodiment of the application provides another combination with a preferable mixture ratio, which comprises the following raw materials in parts by weight: 27 to 36 parts of coptis chinensis, 20 to 29 parts of scutellaria baicalensis, 30 to 50 parts of rheum officinale, 30 to 50 parts of angelica, 28 to 37 parts of radix paeoniae alba, 16 to 22 parts of sophora japonica, 5 to 13 parts of gardenia, 12 to 16 parts of rheum officinale, 25 to 32 parts of philippine violet herb, 15 to 23 parts of evodia rutaecarpa, 15 to 23 parts of costustoot, 12 to 19 parts of red halloysite and 10 to 18 parts of pomegranate bark. The method comprises the following steps: the coptis and the scutellaria clear heat and dry dampness, purge fire and remove toxin, and the angelica and the white paeony root enrich blood and nourish blood, astringe yin and relieve pain, which are monarch drugs; rhubarb, radix et rhizoma Rhei purges heat and purges intestines, eliminates dampness-heat accumulation in stomach and intestine, is matched with flos Sophorae Immaturus, fructus Gardeniae, radix Rumicis, and herba Violae to clear heat and calm liver, cool blood and stop bleeding, and relieve swelling and remove toxicity, and is used as a ministerial drug; evodia rutaecarpa and aucklandia root, radix aucklandiae, as adjuvant drugs, have the effects of warming the middle-jiao to alleviate pain, regulating the flow of qi to arrest vomiting, halloysitum rubrum and pomegranate rind, relieving diarrhea with astringents, promoting granulation and healing wound. In the embodiment of the application, the further preferable mixture ratio of the raw materials is as follows: 29 to 34 parts of coptis chinensis, 25 to 27 parts of scutellaria baicalensis, 35 to 45 parts of rheum officinale, 35 to 45 parts of angelica, 30 to 34 parts of white paeony root, 18 to 20 parts of sophora flower, 7 to 11 parts of gardenia, 13 to 15 parts of rheum officinale, 28 to 30 parts of Chinese violet, 17 to 20 parts of evodia rutaecarpa, 17 to 20 parts of costustoot, 15 to 17 parts of red halloysite and 12 to 16 parts of pomegranate bark. The more preferable mixture ratio is: 32 parts of coptis chinensis, 26 parts of scutellaria baicalensis, 40 parts of rheum officinale, 40 parts of angelica sinensis, 32 parts of radix paeoniae alba, 19 parts of sophora flower, 9 parts of gardenia, 14 parts of rheum officinale, 29 parts of Chinese violet, 18 parts of fructus evodiae, 18 parts of elecampane, 16 parts of red halloysite and 14 parts of pomegranate bark.

In order to further facilitate clinical use, the embodiment of the invention provides a suppository for preventing and treating canine parvoviral enteritis, and the active ingredients of the suppository comprise the traditional Chinese medicine composition for preventing and treating canine parvoviral enteritis.

The medicinal materials are processed and extracted by different methods, and the property and the effect of the medicinal materials can be changed. Different processing and extracting methods also have different requirements on production conditions. The invention provides two preparation methods to prepare the suppository for preventing and treating canine parvovirus enteritis by combining clinical curative effect, production cost and feasibility.

The first preparation method specifically comprises the following operations:

weighing the raw materials in the traditional Chinese medicine composition for preventing and treating canine parvovirus enteritis according to the weight part ratio, decocting and extracting the raw materials with water, concentrating the obtained extracting solution, and drying to prepare traditional Chinese medicine extract powder; melting semisynthetic fatty glyceride, adding the Chinese medicinal extract powder, mixing, and molding.

In the embodiment, the decoction can be carried out for 1 to 3 times, 1.5 to 2h each time. The semisynthetic fatty glyceride can be selected from semisynthetic fatty glyceride commonly used in suppository such as coconut oil ester, palm oil ester, pungent litse fruit oil ester or propylene glycol stearate.

The second preparation method specifically includes the following operations:

weighing the raw materials in the traditional Chinese medicine composition according to the weight part ratio, wherein the effect of the angelica is better when the angelica is fried by carbon;

reflux-extracting Coptidis rhizoma with 0.5% -1.0% v/v sulfuric acid aqueous solution to obtain extractive solution and Coptidis rhizoma residue, adjusting pH of the extractive solution with concentrated hydrochloric acid to 1 to 1.5, filtering, washing the obtained filter cake with water to neutrality, and drying to obtain Coptidis rhizoma first extract; placing the rhizoma Coptidis residue in water, performing enzymolysis with cellulase, filtering, concentrating the filtrate, and drying to obtain a second extract of rhizoma Coptidis;

reflux-extracting radix Angelicae sinensis (parched with charcoal) with ethanol water solution, filtering, concentrating the filtrate, and drying to obtain radix Angelicae sinensis extract;

ultrasonically extracting radix Paeoniae alba with ethanol water solution to obtain radix Paeoniae alba ethanol extractive solution and radix Paeoniae alba residue, extracting radix Paeoniae alba residue with water under reflux to obtain radix Paeoniae alba water extractive solution, concentrating the radix Paeoniae alba ethanol extractive solution and radix Paeoniae alba water extractive solution respectively, drying, and mixing to obtain radix Paeoniae alba extract;

reflux-extracting Scutellariae radix with acetic acid water solution to obtain Scutellariae radix acid extractive solution and Scutellariae radix residue, reflux-extracting Scutellariae radix residue with ethanol water solution to obtain Scutellariae radix ethanol extractive solution, concentrating Scutellariae radix acid extractive solution and Scutellariae radix ethanol extractive solution respectively, drying, and mixing to obtain Scutellariae radix extract;

reflux-extracting rhubarb with a sodium bicarbonate aqueous solution with the pH value of 7.5-8.0, concentrating the obtained extracting solution, and drying to obtain a rhubarb extract;

mixing the first extract of Coptidis rhizoma, the second extract of Coptidis rhizoma, the extract of radix Angelicae sinensis, the extract of radix Paeoniae alba, the extract of Scutellariae radix, the extract of radix et rhizoma Rhei and the mixed extract to obtain Chinese medicinal extract powder; melting semisynthetic fatty glyceride, adding the Chinese medicinal extract powder, mixing, and molding.

The suppository prepared by the preparation method has better curative effect than the suppository prepared by the first preparation method, and particularly has higher cure rate and faster effect on puppies.

In the embodiment, when the dosage of the cellulase is preferably 0.5-1.5% of the mass of the coptis chinensis residue, the enzymolysis temperature is 35-45 ℃, and the enzymolysis time is 3-5 hours; when the aqueous ethanol solution used to prepare the Angelica sinensis extract has a concentration of 50% -60% v/v, and contains 1.5% -3.5% m/m sodium chloride; when the concentration of the ethanol water solution for preparing the white peony root extract is 45% -60% v/v, the parameters of ultrasonic extraction are 45-50Hz, and 240-250W; or when the concentration of the aqueous acetic acid solution for preparing the Scutellariae radix extract is 0.15% to 0.25% v/v, the concentration of the aqueous ethanol solution is 40% to 60% v/v, the therapeutic effect can be further improved.

The following examples are provided to further illustrate the present invention in terms of several examples, and some comparative experiments and animal experiments are listed to verify the prevention and treatment effect of the present invention on canine parvoviral enteritis.

Example 1

The embodiment of the invention provides a traditional Chinese medicine composition for preventing and treating canine parvovirus enteritis, which comprises the following raw materials: 31 parts of coptis chinensis, 25 parts of scutellaria baicalensis, 40 parts of rheum officinale, 40 parts of angelica sinensis, 33 parts of radix paeoniae alba, 12 parts of lotus plumule, 13 parts of gardenia, 13 parts of rheum officinale, 16 parts of herba patriniae, 50 parts of perilla stem and 25 parts of gallnut.

Example 2

The embodiment of the invention provides a traditional Chinese medicine composition for preventing and treating canine parvovirus enteritis, which comprises the following raw materials: 30 parts of coptis chinensis, 23 parts of scutellaria baicalensis, 35 parts of rheum officinale, 35 parts of angelica sinensis, 31 parts of radix paeoniae alba, 11 parts of lotus plumule, 10 parts of gardenia, 12 parts of rheum officinale, 14 parts of herba patriniae, 45 parts of perilla stem and 22 parts of gallnut.

Example 3

The embodiment of the invention provides a traditional Chinese medicine composition for preventing and treating canine parvovirus enteritis, which comprises the following raw materials: 33 parts of coptis chinensis, 26 parts of scutellaria baicalensis, 45 parts of rheum officinale, 45 parts of angelica sinensis, 35 parts of radix paeoniae alba, 13 parts of lotus plumule, 14 parts of gardenia, 15 parts of rheum officinale, 17 parts of herba patriniae, 55 parts of perilla stem and 27 parts of gallnut.

Example 4

The embodiment of the invention provides a traditional Chinese medicine composition for preventing and treating canine parvovirus enteritis, which comprises the following raw materials: 27 parts of coptis chinensis, 20 parts of scutellaria baicalensis, 30 parts of rheum officinale, 30 parts of angelica sinensis, 28 parts of radix paeoniae alba, 10 parts of lotus plumule, 8 parts of gardenia, 10 parts of rheum officinale, 12 parts of herba patriniae, 40 parts of perilla stem and 20 parts of gallnut.

Example 5

The embodiment of the invention provides a traditional Chinese medicine composition for preventing and treating canine parvovirus enteritis, which comprises the following raw materials: 36 parts of coptis chinensis, 29 parts of scutellaria baicalensis, 50 parts of rheum officinale, 50 parts of angelica sinensis, 37 parts of radix paeoniae alba, 15 parts of lotus plumule, 17 parts of gardenia, 17 parts of rheum officinale, 18 parts of herba patriniae, 60 parts of perilla stem and 30 parts of gallnut.

Example 6

The embodiment of the invention provides a traditional Chinese medicine composition for preventing and treating canine parvovirus enteritis, which comprises the following raw materials: 32 parts of coptis chinensis, 24 parts of scutellaria baicalensis, 40 parts of rheum officinale, 40 parts of angelica sinensis, 33 parts of radix paeoniae alba, 29 parts of sophora flower, 19 parts of picria felterrae lour, 8 parts of Chinese violet, 18 parts of cacumen biotae, 36 parts of elecampane and 20 parts of pomegranate bark.

Example 7

The embodiment of the invention provides a traditional Chinese medicine composition for preventing and treating canine parvovirus enteritis, which comprises the following raw materials: 30 parts of coptis chinensis, 23 parts of scutellaria baicalensis, 35 parts of rheum officinale, 35 parts of angelica sinensis, 31 parts of radix paeoniae alba, 27 parts of sophora flower, 17 parts of picria felterrae lour, 7 parts of Chinese violet, 17 parts of cacumen biotae, 34 parts of elecampane and 19 parts of pomegranate rind.

Example 8

The embodiment of the invention provides a traditional Chinese medicine composition for preventing and treating canine parvovirus enteritis, which comprises the following raw materials: 33 parts of coptis chinensis, 26 parts of scutellaria baicalensis, 45 parts of rheum officinale, 45 parts of angelica sinensis, 35 parts of radix paeoniae alba, 30 parts of sophora flower, 21 parts of picria felterrae lour, 10 parts of philippine violet herb, 20 parts of cacumen biotae, 39 parts of elecampane and 21 parts of pomegranate bark.

Example 9

The embodiment of the invention provides a traditional Chinese medicine composition for preventing and treating canine parvovirus enteritis, which comprises the following raw materials: 27 parts of coptis chinensis, 20 parts of scutellaria baicalensis, 30 parts of rheum officinale, 30 parts of angelica sinensis, 28 parts of radix paeoniae alba, 25 parts of sophora flower, 15 parts of picria felterrae lour, 5 parts of Chinese violet, 15 parts of cacumen biotae, 30 parts of elecampane and 18 parts of pomegranate bark.

Example 10

The embodiment of the invention provides a traditional Chinese medicine composition for preventing and treating canine parvovirus enteritis, which comprises the following raw materials: 36 parts of coptis chinensis, 29 parts of scutellaria baicalensis, 50 parts of rheum officinale, 50 parts of angelica sinensis, 37 parts of radix paeoniae alba, 32 parts of sophora flower, 23 parts of picria felterrae lour, 12 parts of Chinese violet, 22 parts of cacumen biotae, 42 parts of elecampane and 22 parts of pomegranate bark.

Example 11

The embodiment of the invention provides a traditional Chinese medicine composition for preventing and treating canine parvovirus enteritis, which comprises the following raw materials: 32 parts of coptis chinensis, 24 parts of scutellaria baicalensis, 40 parts of rheum officinale, 40 parts of angelica sinensis, 32 parts of radix paeoniae alba, 28 parts of sophora flower, 28 parts of herba patriniae, 20 parts of cacumen biotae, 34 parts of fructus evodiae and 20 parts of pomegranate rind.

Example 12

The embodiment of the invention provides a traditional Chinese medicine composition for preventing and treating canine parvovirus enteritis, which comprises the following raw materials: 30 parts of coptis chinensis, 22 parts of scutellaria baicalensis, 35 parts of rheum officinale, 35 parts of angelica sinensis, 30 parts of radix paeoniae alba, 26 parts of sophora flower, 26 parts of patrinia, 18 parts of cacumen biotae, 32 parts of fructus evodiae and 18 parts of pomegranate rind.

Example 13

The embodiment of the invention provides a traditional Chinese medicine composition for preventing and treating canine parvovirus enteritis, which comprises the following raw materials: 34 parts of coptis chinensis, 26 parts of scutellaria baicalensis, 45 parts of rheum officinale, 45 parts of angelica sinensis, 34 parts of radix paeoniae alba, 30 parts of sophora flower, 30 parts of patrinia, 22 parts of cacumen biotae, 36 parts of fructus evodiae and 22 parts of pomegranate rind.

Example 14

The embodiment of the invention provides a traditional Chinese medicine composition for preventing and treating canine parvovirus enteritis, which comprises the following raw materials: 27 parts of coptis chinensis, 20 parts of scutellaria baicalensis, 30 parts of rheum officinale, 30 parts of angelica sinensis, 28 parts of radix paeoniae alba, 25 parts of sophora flower, 23 parts of herba patriniae, 16 parts of cacumen biotae, 30 parts of fructus evodiae and 18 parts of pomegranate bark.

Example 15

The embodiment of the invention provides a traditional Chinese medicine composition for preventing and treating canine parvovirus enteritis, which comprises the following raw materials: 36 parts of coptis chinensis, 29 parts of scutellaria baicalensis, 50 parts of rheum officinale, 50 parts of angelica sinensis, 37 parts of radix paeoniae alba, 32 parts of sophora flower, 32 parts of herba patriniae, 23 parts of cacumen biotae, 38 parts of fructus evodiae and 22 parts of pomegranate bark.

Example 16

The embodiment of the invention provides a traditional Chinese medicine composition for preventing and treating canine parvovirus enteritis, which comprises the following raw materials: 32 parts of coptis chinensis, 26 parts of scutellaria baicalensis, 40 parts of rheum officinale, 40 parts of angelica sinensis, 32 parts of radix paeoniae alba, 19 parts of sophora flower, 9 parts of gardenia, 14 parts of rheum officinale, 29 parts of Chinese violet, 18 parts of fructus evodiae, 18 parts of elecampane, 16 parts of red halloysite and 14 parts of pomegranate bark.

Example 17

The embodiment of the invention provides a traditional Chinese medicine composition for preventing and treating canine parvovirus enteritis, which comprises the following raw materials: 29 parts of coptis root, 25 parts of scutellaria baicalensis, 35 parts of rhubarb, 35 parts of angelica, 30 parts of white peony root, 18 parts of sophora flower, 7 parts of gardenia, 13 parts of rhubarb root and rhizome, 28 parts of Chinese violet, 17 parts of evodia rutaecarpa, 17 parts of costustoot, 15 parts of red halloysite and 12 parts of pomegranate bark.

Example 18

The embodiment of the invention provides a traditional Chinese medicine composition for preventing and treating canine parvovirus enteritis, which comprises the following raw materials: 34 parts of coptis chinensis, 27 parts of scutellaria baicalensis, 45 parts of rheum officinale, 45 parts of angelica sinensis, 34 parts of radix paeoniae alba, 20 parts of sophora japonica, 11 parts of gardenia, 15 parts of rheum officinale, 30 parts of Chinese violet, 20 parts of fructus evodiae, 20 parts of elecampane, 17 parts of red halloysite and 16 parts of pomegranate bark.

Example 19

The embodiment of the invention provides a traditional Chinese medicine composition for preventing and treating canine parvovirus enteritis, which comprises the following raw materials: 27 parts of coptis chinensis, 20 parts of scutellaria baicalensis, 30 parts of rheum officinale, 30 parts of angelica sinensis, 28 parts of radix paeoniae alba, 16 parts of sophora flower, 5 parts of gardenia, 12 parts of rheum officinale, 25 parts of Chinese violet, 15 parts of fructus evodiae, 15 parts of elecampane, 12 parts of red halloysite and 10 parts of pomegranate bark.

Example 20

The embodiment of the invention provides a traditional Chinese medicine composition for preventing and treating canine parvovirus enteritis, which comprises the following raw materials: 36 parts of coptis chinensis, 29 parts of scutellaria baicalensis, 50 parts of rheum officinale, 50 parts of angelica sinensis, 37 parts of radix paeoniae alba, 22 parts of sophora flower, 13 parts of gardenia, 16 parts of rheum officinale, 32 parts of Chinese violet, 23 parts of fructus evodiae, 23 parts of elecampane, 19 parts of red halloysite and 18 parts of pomegranate bark.

Example 21

The embodiment of the invention provides a suppository for preventing and treating canine parvovirus enteritis, which comprises the traditional Chinese medicine composition of the embodiment 1 and coco oil.

The preparation method of the suppository comprises the following steps:

weighing the raw materials in the traditional Chinese medicine composition of the embodiment 1 according to the weight part ratio, adding water, decocting for 2 times, 2 hours for the first time and 1.5 hours for the 2 nd time, combining the decoctions, filtering, concentrating, spraying powder for later use. Heating coconut oil ester in 100 deg.C water bath to melt, adding the Chinese medicinal powder, stirring, molding, and making into suppository with length of about 2cm, height of about 0.85cm, and width of about 1cm, and drug content of 0.3g (calculated on crude drug) in each suppository.

Example 22

The embodiment of the invention provides a suppository for preventing and treating canine parvovirus enteritis, which comprises the traditional Chinese medicine composition of the embodiment 6 and coco oil.

The preparation method of the suppository comprises the following steps:

weighing the raw materials in the traditional Chinese medicine composition of the embodiment 6 according to the weight part ratio, adding water, decocting for 2 times, 2 hours for the first time and 1.5 hours for the 2 nd time, combining the decoctions, filtering, concentrating, spraying powder for later use. Heating coconut oil ester in 100 deg.C water bath to melt, adding the Chinese medicinal powder, stirring, molding, and making into suppository with length of about 2cm, height of about 0.85cm, and width of about 1cm, and drug content of 0.3g (calculated on crude drug) in each suppository.

Example 23

The embodiment of the invention provides a suppository for preventing and treating canine parvovirus enteritis, which comprises the traditional Chinese medicine composition of the embodiment 11 and coco oil.

The preparation method of the suppository comprises the following steps:

weighing the raw materials in the traditional Chinese medicine composition of the embodiment 11 according to the weight part ratio, adding water, decocting for 2 times, 2 hours for the first time and 1.5 hours for the 2 nd time, combining the decoctions, filtering, concentrating and spraying powder for later use. Heating coconut oil ester in 100 deg.C water bath to melt, adding the Chinese medicinal powder, stirring, molding, and making into suppository with length of about 2cm, height of about 0.85cm, and width of about 1cm, and drug content of 0.3g (calculated on crude drug) in each suppository.

Example 24

The embodiment of the invention provides a suppository for preventing and treating canine parvovirus enteritis, which comprises the traditional Chinese medicine composition in the embodiment 16 and coco oil.

The preparation method of the suppository comprises the following steps:

weighing the raw materials in the traditional Chinese medicine composition of the embodiment 16 according to the weight part ratio, adding water, decocting for 2 times, 2 hours for the first time and 1.5 hours for the 2 nd time, combining the decoctions, filtering, concentrating, spraying powder for later use. Heating coconut oil ester in 100 deg.C water bath to melt, adding the Chinese medicinal powder, stirring, molding, and making into suppository with length of about 2cm, height of about 0.85cm, and width of about 1cm, and drug content of 0.3g (calculated on crude drug) in each suppository.

Example 25

The embodiment of the invention provides a suppository for preventing and treating canine parvovirus enteritis, which comprises the traditional Chinese medicine composition in the embodiment 1 and coco oil.

The preparation method of the suppository comprises the following steps:

weighing the raw materials in the traditional Chinese medicine composition in the embodiment 1 according to the weight part ratio, wherein the angelica sinensis is stir-fried with charcoal;

reflux-extracting Coptidis rhizoma with 7 times of 0.75% (v/v) sulfuric acid aqueous solution to obtain extractive solution and Coptidis rhizoma residue, adjusting pH of the extractive solution to 1.2 with concentrated hydrochloric acid, filtering, washing the obtained filter cake with water to pH7, and drying to obtain first extract of Coptidis rhizoma; putting the coptis chinensis dregs in 6 times of water, performing enzymolysis for 4 hours at 35-45 ℃ by using cellulase accounting for 1.0% of the mass of the coptis chinensis dregs, heating to 90-95 ℃, keeping for 10 minutes to inactivate the enzyme, filtering, concentrating the filtrate at 45-55 ℃, and drying to obtain a second coptis chinensis extract;

reflux-extracting radix Angelicae sinensis with 8 times of 55% v/v ethanol water solution containing 2.5% m/m sodium chloride, filtering, concentrating the filtrate at 45-55 deg.C, and drying to obtain radix Angelicae sinensis extract;

ultrasonically extracting radix Paeoniae alba with 8 times of 55 v/v ethanol aqueous solution for 1h,45Hz and 240W to obtain radix Paeoniae alba ethanol extractive solution and radix Paeoniae alba residue, reflux-extracting radix Paeoniae alba residue with 5 times of water for 1.5h to obtain radix Paeoniae alba water extractive solution, concentrating the radix Paeoniae alba ethanol extractive solution and radix Paeoniae alba water extractive solution at 45-55 deg.C, drying, and mixing to obtain radix Paeoniae alba extract;

reflux-extracting Scutellariae radix with 6 times of 0.2% v/v acetic acid water solution for 1.5 hr to obtain Scutellariae radix acid extractive solution and Scutellariae radix residue, reflux-extracting Scutellariae radix residue with 6 times of 50% v/v ethanol water solution for 1.5 hr to obtain Scutellariae radix ethanol extractive solution, concentrating Scutellariae radix acid extractive solution and Scutellariae radix ethanol extractive solution at 45-55 deg.C respectively, drying, and mixing to obtain Scutellariae radix extract;

reflux-extracting radix et rhizoma Rhei with 7 times of sodium bicarbonate water solution of pH7.8 for 1.5h, concentrating the obtained extractive solution at 45-55 deg.C, and drying to obtain radix et rhizoma Rhei extract;

mixing the rest raw materials, performing reflux extraction by using water of which the amount is 7 times that of the mixture, concentrating the obtained extracting solution at 45 to 55 ℃, and drying to obtain a mixed extract;

mixing the first extract of Coptidis rhizoma, the second extract of Coptidis rhizoma, the extract of radix Angelicae sinensis, the extract of radix Paeoniae alba, the extract of Scutellariae radix, the extract of radix et rhizoma Rhei and the mixed extract to obtain Chinese medicinal extract powder; melting coconut oil ester, adding the Chinese medicinal extract powder, mixing, molding, and making into suppository with length of about 2cm, height of about 0.85cm, and width of about 1cm, and drug content of 0.3g (calculated on crude drug) in each suppository.

Example 26

The embodiment of the invention provides a suppository for preventing and treating canine parvovirus enteritis, which comprises the traditional Chinese medicine composition prepared in the embodiment 1 and coco oil.

The preparation method of the suppository comprises the following steps:

reflux-extracting Coptidis rhizoma with 7 times of 0.5% v/v sulfuric acid aqueous solution to obtain extractive solution and Coptidis rhizoma residue, adjusting pH of the extractive solution to 1 with concentrated hydrochloric acid, filtering, washing the filter cake with water to pH7, and drying to obtain Coptidis rhizoma first extract; putting the coptis chinensis dregs in 7 times of water, performing enzymolysis for 5 hours at 35-45 ℃ by using cellulase accounting for 0.5% of the mass of the coptis chinensis dregs, heating to 90-95 ℃, keeping for 10 minutes to inactivate the enzyme, filtering, concentrating the filtrate at 45-55 ℃, and drying to obtain a second coptis chinensis extract;

reflux-extracting radix Angelicae sinensis with 8 times of 50% v/v ethanol water solution containing 1.5% m/m sodium chloride, filtering, concentrating the filtrate at 45-55 deg.C, and drying to obtain radix Angelicae sinensis extract;

ultrasonically extracting radix paeoniae alba by using 8 times of 45% v/v ethanol aqueous solution for 1h,45Hz and 240W to obtain radix paeoniae alba ethanol extract and radix paeoniae alba residues, refluxing and extracting the radix paeoniae alba residues by using 5 times of water for 1.2h to obtain radix paeoniae alba water extract, concentrating and drying the radix paeoniae alba ethanol extract and the radix paeoniae alba water extract at 45-55 ℃, and mixing to obtain the radix paeoniae alba extract;

reflux-extracting Scutellariae radix with 6 times of 0.15% m/m acetic acid water solution for 2h to obtain Scutellariae radix acid extractive solution and Scutellariae radix residue, reflux-extracting Scutellariae radix residue with 6 times of 40% v/v ethanol water solution for 2h to obtain Scutellariae radix ethanol extractive solution, concentrating Scutellariae radix acid extractive solution and Scutellariae radix ethanol extractive solution at 45 deg.C to 55 deg.C, drying, and mixing to obtain Scutellariae radix extract;

reflux-extracting radix et rhizoma Rhei with 8 times of sodium bicarbonate water solution of pH7.5 for 2 hr, concentrating the obtained extractive solution at 45-55 deg.C, and drying to obtain radix et rhizoma Rhei extract;

mixing the rest raw materials, performing reflux extraction by using 8 times of water, concentrating the obtained extracting solution at 45 to 55 ℃, and drying to obtain a mixed extract;

Example 27

The preparation method of the suppository comprises the following steps:

reflux-extracting Coptidis rhizoma with 7 times of 1.0 v/v sulfuric acid aqueous solution to obtain extractive solution and Coptidis rhizoma residue, adjusting pH of the extractive solution to 1.5 with concentrated hydrochloric acid, filtering, washing the filter cake with water to pH7, and drying to obtain Coptidis rhizoma first extract; putting the coptis chinensis residues into water with the amount of 5 times that of the coptis chinensis residues, performing enzymolysis for 3 hours at 35-45 ℃ by using cellulase with the mass being 1.5% of that of the coptis chinensis residues, heating to 90-95 ℃, keeping for 10 minutes to inactivate the enzyme, filtering, concentrating the filtrate at 45-55 ℃, and drying to obtain a second coptis chinensis extract;

reflux-extracting radix Angelicae sinensis with 8 times of 60% v/v ethanol water solution containing 3.5% m/m sodium chloride, filtering, concentrating the filtrate at 45-55 deg.C, and drying to obtain radix Angelicae sinensis extract;

ultrasonically extracting radix Paeoniae alba with 8 times of 60 v/v ethanol aqueous solution for 1h,50Hz and 250W to obtain radix Paeoniae alba ethanol extractive solution and radix Paeoniae alba residue, reflux-extracting radix Paeoniae alba residue with 5 times of water for 1.5h to obtain radix Paeoniae alba water extractive solution, concentrating the radix Paeoniae alba ethanol extractive solution and radix Paeoniae alba water extractive solution at 45-55 deg.C, drying, and mixing to obtain radix Paeoniae alba extract;

reflux-extracting Scutellariae radix with 6 times of 0.25% (v/v) acetic acid water solution for 1.5 hr to obtain Scutellariae radix acid extractive solution and Scutellariae radix residue, reflux-extracting Scutellariae radix residue with 6 times of 60% (v/v) ethanol water solution for 1.5 hr to obtain Scutellariae radix ethanol extractive solution, concentrating Scutellariae radix acid extractive solution and Scutellariae radix ethanol extractive solution at 45-55 deg.C respectively, drying, and mixing to obtain Scutellariae radix extract;

reflux-extracting radix et rhizoma Rhei with 7 times of sodium bicarbonate water solution of pH8.0 for 1.5 hr, concentrating the obtained extractive solution at 45-55 deg.C, and drying to obtain radix et rhizoma Rhei extract;

mixing the first extract of Coptidis rhizoma, the second extract of Coptidis rhizoma, the extract of Angelicae Gigantis radix, radix Paeoniae alba, scutellariae radix, and radix et rhizoma Rhei, and the mixed extract, and making into Chinese medicinal extract powder; melting coconut oil ester, adding the Chinese medicinal extract powder, mixing, molding, and making into suppository with length of about 2cm, height of about 0.85cm, and width of about 1cm, and drug content of 0.3g (calculated on crude drug) per suppository.

Example 28

The embodiment of the invention provides a suppository for preventing and treating canine parvovirus enteritis, which comprises the traditional Chinese medicine composition in the embodiment 6 and coco oil. The preparation method and specification of the suppository are the same as those of example 25.

Example 29

The embodiment of the invention provides a suppository for preventing and treating canine parvovirus enteritis, which comprises the traditional Chinese medicine composition in the embodiment 6 and coco oil. The preparation method and specification of the suppository are the same as those of example 26.

Example 30

The embodiment of the invention provides a suppository for preventing and treating canine parvovirus enteritis, which comprises the traditional Chinese medicine composition in the embodiment 6 and coco oil. The suppository is prepared by the same method and specification as example 27.

Example 31

The embodiment of the invention provides a suppository for preventing and treating canine parvovirus enteritis, which comprises the traditional Chinese medicine composition in the embodiment 11 and coco oil. The preparation method and specification of the suppository are the same as those of example 25.

Example 32

The embodiment of the invention provides a suppository for preventing and treating canine parvovirus enteritis, which comprises the traditional Chinese medicine composition in the embodiment 11 and coco oil. The suppository is prepared by the same method and specification as example 26.

Example 33

The embodiment of the invention provides a suppository for preventing and treating canine parvovirus enteritis, which comprises the traditional Chinese medicine composition in the embodiment 11 and coco oil. The suppository is prepared by the same method and specification as example 27.

Example 34

The embodiment of the invention provides a suppository for preventing and treating canine parvovirus enteritis, which comprises the traditional Chinese medicine composition in the embodiment 16 and coco oil. The preparation method and specification of the suppository are the same as those of example 25.

Example 35

The embodiment of the invention provides a suppository for preventing and treating canine parvovirus enteritis, which comprises the traditional Chinese medicine composition of the embodiment 16 and coco oil. The preparation method and specification of the suppository are the same as those of example 26.

Example 36

The embodiment of the invention provides a suppository for preventing and treating canine parvovirus enteritis, which comprises the traditional Chinese medicine composition of the embodiment 16 and coco oil. The preparation method and specification of the suppository are the same as those of example 27.

Comparative example 1

The present comparative example provides a suppository, the raw materials of which are a traditional Chinese medicine composition and coconut oil ester, the traditional Chinese medicine composition is based on example 1, and coptis chinensis and scutellaria baicalensis are changed into phellodendron. The method specifically comprises the following raw materials: 56 parts of phellodendron, 40 parts of rhubarb, 40 parts of angelica, 33 parts of white peony root, 12 parts of lotus plumule, 13 parts of gardenia, 13 parts of rhubarb, 16 parts of herba patriniae, 50 parts of perilla stem and 25 parts of gallnut.

The preparation method and specifications were the same as in example 25.

Comparative example 2

The present comparative example provides a suppository, the raw materials of which are a traditional Chinese medicine composition and coconut oil ester, the traditional Chinese medicine composition is based on example 6, and coptis chinensis and scutellaria baicalensis are changed into phellodendron. The method specifically comprises the following raw materials: 56 parts of phellodendron, 40 parts of rhubarb, 40 parts of angelica, 33 parts of white peony root, 29 parts of sophora flower, 19 parts of picria felterrae lour, 8 parts of Chinese violet, 18 parts of cacumen biotae, 36 parts of costus root and 20 parts of pomegranate bark.

The preparation method and the specification are the same as those of example 25.

Comparative example 3

The present comparative example provides a suppository, the raw materials of which are a traditional Chinese medicine composition and coconut oil ester, the traditional Chinese medicine composition is based on example 11, and coptis chinensis and scutellaria baicalensis are changed into phellodendron. The method specifically comprises the following raw materials: 56 parts of phellodendron, 40 parts of rhubarb, 40 parts of angelica, 32 parts of white peony root, 28 parts of sophora flower, 28 parts of herba patriniae, 20 parts of cacumen biotae, 34 parts of fructus evodiae and 20 parts of pomegranate bark.

The preparation method and specifications were the same as in example 25.

Comparative example 4

The present comparative example provides a suppository, the raw materials of which are a traditional Chinese medicine composition and coconut oil ester, the traditional Chinese medicine composition is based on example 16, and coptis chinensis and scutellaria baicalensis are changed into phellodendron. The preparation method specifically comprises the following raw materials: 58 parts of phellodendron bark, 40 parts of rhubarb, 40 parts of angelica, 32 parts of white peony root, 19 parts of sophora flower, 9 parts of gardenia, 14 parts of rhubarb root, 29 parts of Chinese violet, 18 parts of evodia fruit, 18 parts of costus root, 16 parts of red halloysite and 14 parts of pomegranate rind.

The preparation method and specifications were the same as in example 25.

Comparative example 5

The present comparative example provides a suppository, the raw materials of which are a traditional Chinese medicine composition and coconut oil ester, the traditional Chinese medicine composition is based on example 1, and rheum officinale is changed into senna leaves. The method specifically comprises the following raw materials: 31 parts of coptis chinensis, 25 parts of scutellaria baicalensis, 40 parts of senna leaves, 40 parts of angelica sinensis, 33 parts of white paeony root, 12 parts of lotus plumule, 13 parts of gardenia, 13 parts of rhubarb root and rhizome, 16 parts of herba patriniae, 50 parts of perilla stem and 25 parts of Chinese gall.

The preparation method and specifications were the same as in example 25.

Comparative example 6

The present comparative example provides a suppository, the raw materials of which are a traditional Chinese medicine composition and coconut oil ester, the traditional Chinese medicine composition is based on example 6, and rheum officinale is changed into senna leaves. The preparation method specifically comprises the following raw materials: 32 parts of coptis chinensis, 24 parts of scutellaria baicalensis, 40 parts of senna leaves, 40 parts of angelica sinensis, 33 parts of white paeony root, 29 parts of sophora flower, 19 parts of picria felterrae lour, 8 parts of Chinese violet, 18 parts of cacumen biotae, 36 parts of costus root and 20 parts of pomegranate bark.

Comparative example 7

The present comparative example provides a suppository, the raw materials of which are a traditional Chinese medicine composition and coconut oil ester, the traditional Chinese medicine composition is based on example 11, and rheum officinale is changed into senna leaves. The method specifically comprises the following raw materials: 32 parts of coptis chinensis, 24 parts of scutellaria baicalensis, 40 parts of folium sennae, 40 parts of angelica sinensis, 32 parts of radix paeoniae alba, 28 parts of flos sophorae, 28 parts of herba patriniae, 20 parts of cacumen biotae, 34 parts of fructus evodiae and 20 parts of pomegranate rind.

The preparation method and specifications were the same as in example 25.

Comparative example 8

The present comparative example provides a suppository, the raw materials of which are a traditional Chinese medicine composition and coconut oil ester, the traditional Chinese medicine composition is based on example 16, and rheum officinale is changed into senna leaves. The preparation method specifically comprises the following raw materials: 32 parts of coptis chinensis, 26 parts of scutellaria baicalensis, 40 parts of folium sennae, 40 parts of angelica sinensis, 32 parts of radix paeoniae alba, 19 parts of sophora flower, 9 parts of gardenia, 14 parts of rheum officinale, 29 parts of Chinese violet, 18 parts of fructus evodiae, 18 parts of elecampane, 16 parts of red halloysite and 14 parts of pomegranate bark.

Effect example 1

A plurality of sick dogs and experimental dogs (all small dogs, and all dogs are small dogs of 12 months old or less and all are canine parvovirus enteritis diagnosed by diagnosis) which are harvested in animal hospitals from 2019 to 2021 in 6 months are selected, and the clinical manifestations of the sick dogs and the experimental dogs are diarrhea, vomiting and the like in different degrees.

The above disease dogs were divided into 17 groups, and the breed distribution of disease dogs with the same infection degree in each group was the same (for example, each group of severely infected disease dogs were 2 bomei dogs, 2 coxy dogs, 2 teddy dogs, and 2 barger dogs), and the breed was hermaphrodite and hermaphrodite in each group, and the age of the month was similar. The dogs in each group were treated with the suppositories obtained in examples 21, 22, 23, 24, 25, 28, 31 and 34 and comparative examples 1 to 8. The rectal administration was performed 1 time, one at a time, after defecation in the morning dogs. Blank control group was given one blank suppository. The blank suppository is prepared by the same method only by using the same matrix without adding traditional Chinese medicines. The same dog food is normally fed during the administration period, and the water is freely drunk.

The above dogs are respectively in different infection degrees, and the clinical characteristics are respectively as follows:

mild: a little depression, reduced appetite, excessive lying and less movement, vomiting and diarrhea, vomitus which is chyme and yellow gastric juice, thick excrement, stink and less urine, and the body temperature is 40 to 41 ℃ or normal;

medium: the appetite is exhausted, vomit is yellow or green mucus-like, or only vomit action is performed without vomit, the body temperature is not high or is higher than 40 ℃ for a long time, the excrement is thin-like, intestinal mucosa fragments or bubbles fallen off by mixed oil have special fishy smell, some are jet-shaped, and the tail part is full of fishy smell and thin stool; a decrease in urine volume; the wool is rough and messy and has no luster;

and (2) the severity: intractable vomiting and diarrhea, some of gastrointestinal hemorrhage, thready and weak pulse of medial femoral artery, cold palpation, body temperature drop, high collapse and exhaustion, lethargy, deep breathing, deep pit, severe dehydration, dull eyes, dry and cracked rhinoscope, poor skin elasticity, emaciation, protruded ribs, walking swing, dirty back hair, scanty urine and yellow color.

Clinical symptoms of the above dogs were counted for 7 days from the day of administration (0 d), and clinical treatment effects were counted according to the following criteria:

the effect is shown: the body temperature, diet, defecation and heart rate of the light and moderate sick dogs are recovered to be normal within 3d after treatment, and vomiting phenomenon does not occur; after the severe disease dog is treated, the body temperature is raised to 40 ℃ or the body temperature is low, the heart rate is reduced to below 140 times/min, the spirit is gradually recovered, a little food can be eaten, the excrement is gradually recovered to be normal, the vomiting symptom is gradually reduced, and the body temperature is recovered to be normal after the severe disease dog is treated for 3 days;

the method has the following advantages: after the light and medium sick dogs are treated, the body temperature, diet, defecation and heart rate are recovered to be normal within 6 days, and the vomiting phenomenon is avoided; after the severe disease dog is treated, the body temperature drops by more than 1 ℃, the heart rate drops to below 150 times/min, the spirit is gradually recovered, the excrement is gradually recovered, the vomiting symptom is gradually reduced, and the body temperature is recovered after the severe disease dog is treated for 6 days;

and (4) invalidation: no improvement in clinical symptoms or death of the sick dog.

The statistical results of the treatment effect are shown in table 1.

Wherein, the total effective rate = (number of effective cases + number of effective cases)/n × 100%.

TABLE 1 comparison of the therapeutic effects of the groups

The statistical results show that the canine parvovirus enteritis in each example group has higher total effective rate, which is obviously higher than that of a blank control group and than that of each comparative example group, wherein the total effective rate of the example 23 group, the example 31 group and the example 34 group is the highest, and the suppository obtained in the example of the invention has effective treatment effect on canine parvovirus, and the treatment effect of the example 23 group, the example 31 group and the example 34 group is the most obvious.

The traditional Chinese medicine compositions in the examples 2 to 5, 7 to 10, 12 to 15 and 17 to 20 and palm oil ester, litsea cubeba oil ester or propylene glycol stearate are taken as substrates, and the suppository prepared by the preparation method can be prepared into suppositories with the same specification for a small number of cases, and the total effective rate can reach more than 75 percent.

Effect example 2

Three canine farms with canine parvovirus infection cases were selected and fed small, medium and large dogs, respectively. Puppies (males and females) not infected with canine parvovirus and having an age of 4 months or less were administered with the suppositories obtained in examples 21, 22, 23, 24, 25, 28, 31, and 34 and comparative examples 1 to 8, respectively, for prevention. The rectal administration was performed 1 time, one at a time, after defecation in the morning dogs. Blank control group was given one blank suppository. The blank suppository is prepared by the same method only by using the same matrix without adding traditional Chinese medicines. The same dog food was fed normally during dosing, with free access to water.

The number of infections of the puppies within 14d from the day of medication (0 d) is counted, and the results are shown in tables 2 to 4:

TABLE 2 statistics of preventive effect (small dog farm)

Group of	n	Number of infected cases
			Example 21	50	7
Example 22	50	5
			Example 23	50	2
Example 24	50	4
			Example 25	50	5
Example 28	50	4
			Example 31	50	0
Example 34	50	3
			Comparative example 1	50	19
Comparative example 2	50	16
			Comparative example 3	50	14
Comparative example 4	50	17
			Comparative example 5	50	16
Comparative example 6	50	14
			Comparative example 7	50	11
Comparative example 8	50	15
			Blank control group	50	38

TABLE 3 statistics of preventive effect (Medium dog farm)

Group of	n	Number of infected cases
			Example 21	50	6
Example 22	50	6
			Example 23	50	3
Example 24	50	5
			Example 25	50	4
Example 28	50	3
			Example 31	50	0
Example 34	50	3
			Comparative example 1	50	18
Comparative example 2	50	17
			Comparative example 3	50	15
Comparative example 4	50	17
			Comparative example 5	50	15
Comparative example 6	50	15
			Comparative example 7	50	11
Comparative example 8	50	14
			Blank control group	50	35

TABLE 4 statistics of preventive effect (Large dog farm)

Group of	n	Number of infected cases
			Example 21	50	6
Example 22	50	5
			Example 23	50	2
Practice ofExample 24	50	5
			Example 25	50	4
Example 28	50	3
			Example 31	50	0
Example 34	50	4
			Comparative example 1	50	18
Comparative example 2	50	16
			Comparative example 3	50	13
Comparative example 4	50	15
			Comparative example 5	50	16
Comparative example 6	50	13
			Comparative example 7	50	11
Comparative example 8	50	14
			Blank control group	50	33

According to the statistical results, the infection rates of the example groups are 0 to 14 percent and are obviously lower than those of the blank control group and than those of the comparative example groups, wherein the infection rates of the example 23 group and the example 31 group are the lowest, and the suppository obtained in the example of the invention is proved to have excellent prevention effect on canine parvovirus, and the prevention effect of the example 23 and the example 31 is the most obvious.

A small number of cases were examined by using the Chinese medicinal compositions of examples 2 to 5, 7 to 10, 12 to 15, and 17 to 20 and palm oil ester, pungent litse fruit oil ester, or propylene glycol stearate as bases, and preparing suppositories of the same specification by the above-mentioned preparation method, wherein the infection rate was 15% or less.

The above description is intended to be illustrative of the preferred embodiment of the present invention and should not be taken as limiting the invention, but rather, the invention is intended to cover all modifications, equivalents, and alternatives falling within the spirit and scope of the invention.

Claims

1. The traditional Chinese medicine composition for preventing and treating canine parvovirus enteritis is characterized by comprising the following raw materials in parts by weight: 27 to 36 parts of coptis chinensis, 20 to 29 parts of scutellaria baicalensis, 30 to 50 parts of rheum officinale, 30 to 50 parts of angelica sinensis, 28 to 37 parts of white paeony root, 10 to 15 parts of lotus plumule, 8 to 17 parts of gardenia, 10 to 17 parts of rheum officinale, 12 to 18 parts of patrinia scaniosaefolia, 40 to 60 parts of perilla stem and 20 to 30 parts of gallnut.

2. The traditional Chinese medicine composition for preventing and treating canine parvovirus enteritis is characterized by comprising the following raw materials in parts by weight: 27 to 36 parts of coptis chinensis, 20 to 29 parts of scutellaria baicalensis, 30 to 50 parts of rheum officinale, 30 to 50 parts of angelica, 28 to 37 parts of white paeony root, 25 to 32 parts of sophora flower, 15 to 23 parts of picria felterrae lour, 5 to 12 parts of Chinese violet, 15 to 22 parts of cacumen biotae, 30 to 42 parts of costustoot and 18 to 22 parts of pomegranate bark.

3. The traditional Chinese medicine composition for preventing and treating canine parvovirus enteritis is characterized by comprising the following raw materials in parts by weight: 27 to 36 parts of coptis chinensis, 20 to 29 parts of scutellaria baicalensis, 30 to 50 parts of rheum officinale, 30 to 50 parts of angelica, 28 to 37 parts of radix paeoniae alba, 25 to 32 parts of sophora flower, 23 to 32 parts of herba patriniae, 16 to 23 parts of cacumen biotae, 30 to 38 parts of fructus evodiae and 18 to 22 parts of pomegranate bark.

4. The traditional Chinese medicine composition for preventing and treating canine parvovirus enteritis is characterized by comprising the following raw materials in parts by weight: 27 to 36 parts of coptis chinensis, 20 to 29 parts of scutellaria baicalensis, 30 to 50 parts of rheum officinale, 30 to 50 parts of Chinese angelica, 28 to 37 parts of white paeony root, 16 to 22 parts of sophora japonica, 5 to 13 parts of gardenia, 12 to 16 parts of rheum officinale, 25 to 32 parts of Chinese violet, 15 to 23 parts of evodia rutaecarpa, 15 to 23 parts of costustoot, 12 to 19 parts of red halloysite and 10 to 18 parts of pomegranate bark.

5. A suppository for preventing and treating canine parvoviral enteritis, which is characterized in that the active ingredient of the suppository is the traditional Chinese medicine composition for preventing and treating canine parvoviral enteritis according to any one of claims 1 to 4.

6. A preparation method of a suppository for preventing and treating canine parvovirus enteritis is characterized by comprising the following operations:

weighing the raw materials in the traditional Chinese medicine composition for preventing and treating canine parvovirus enteritis according to any one of claims 1 to 4 in parts by weight, decocting the raw materials with water for extraction, concentrating the obtained extract, drying, and preparing the concentrated extract into traditional Chinese medicine extract powder; melting semisynthetic fatty glyceride, adding the Chinese medicinal extract powder, mixing, and molding.

7. A preparation method of a suppository for preventing and treating canine parvovirus enteritis is characterized by comprising the following steps:

weighing the raw materials in the traditional Chinese medicine composition according to any one of claims 1 to 4 in parts by weight, wherein the angelica is carbon-fried angelica;

extracting radix Angelicae sinensis with ethanol water under reflux, filtering, concentrating the filtrate, and drying to obtain radix Angelicae sinensis extract;

reflux-extracting the rhubarb with a sodium bicarbonate aqueous solution with the pH value of 7.5-8.0, concentrating and drying the obtained extracting solution to obtain a rhubarb extract;

mixing the rest raw materials, extracting with water under reflux, concentrating the extractive solution, and drying to obtain mixed extract;

mixing the first extract of Coptidis rhizoma, the second extract of Coptidis rhizoma, the extract of Angelicae Gigantis radix, paeoniae alba, scutellariae radix, and radix et rhizoma Rhei with the mixed extract to obtain Chinese medicinal extract powder; melting semisynthetic fatty glyceride, adding the Chinese medicinal extract powder, mixing, and injection molding.

8. The preparation method of the suppository for preventing and treating canine parvoviral enteritis according to claim 7, wherein the mass of the cellulase is 0.5-1.5% of the mass of the coptis chinensis residue, the enzymolysis temperature is 35-45 ℃, and the enzymolysis time is 3-5h.

9. The method of claim 7, wherein the concentration of the aqueous ethanol solution from which the angelica sinensis extract is prepared is 56% to 60% by volume v/v, the aqueous ethanol solution containing 1.5% to 3.5% by volume m/m sodium chloride; and/or

The concentration of the ethanol aqueous solution for preparing the white peony root extract is 45% -60% v/v, the parameters of ultrasonic extraction are 45% -50Hz, 240% -250W; and/or

The concentration of the aqueous acetic acid solution for preparing the scutellaria baicalensis extract is 0.15% to 0.25% by volume v/v, and the concentration of the aqueous ethanol solution is 40% to 60% by volume v/v.