A kind of compound Chinese medicine extract and application thereof preventing and treating livestock and poultry diarrhea syndrome
Technical field
The present invention relates to animal compound technical field of Chinese medicines, particularly relate to compound Chinese medicine extract of a kind of prevention and therapy livestock and poultry diarrhea syndrome and its preparation method and application.
Background technology
Diarrhoea is one independently disease, it is again a symptom in various diseases pathogenesis, poultry gastroenteritis diarrhea syndrome is a global problem, long-standing problem the development of aquaculture, this disease is popular wide, falls ill all the year round, even if survive dead poultry also often cause poor growth, the price of deed reduces, and causes heavy economic losses.
Modern medicine study shows, diarrhea pathgen is many, pathogenesis is complicated, and its pathogenesis basis is the obstacles such as gastrointestinal secretion, digestion, absorption and motion, and secretory volume is increased, and food can not decompose completely, caused by absorbtivity minimizing and enterogastric peristalsis acceleration etc.Wherein, inflammatory mediator is a class diarrhea inducing autacoid, and most diarrhoeal diseases of clinical findings (comprising infectious and non-infectious) are because virulence factor makes intestinal be inflamed mostly, discharges that various inflammatory mediator causes.Therefore, anti-diarrheal, by antiinflammatory, as synthesis, the release of the generation of inflammation-inhibiting, development or inflammatory mediator, produces antidiarrheal effect.And on veterinary clinic, usually adopt antibacterial drug therapy at present, but because antibacterial can only be antibacterial, but it is invalid to enterotoxin, be difficult to fundamentally treat diarrhoea, improve the function of intestinal canal sexually transmitted disease (STD) change because inflammation causes, add simultaneously and very easily produce drug resistance, make the very strong medicine of some original antibacterial actions progressively become insensitive medicine, bring larger difficulty to the control of disease.
Chinese herbal medicine contains plurality of active ingredients, its effect is Mutiple Targets, except containing except antimicrobial component, also have multiple nutritional components and the active substances such as protein, aminoacid, vitamin, polysaccharide, growth of animal performance can be improved, strengthen gut metabolism movable, strengthen digestive and absorptive functions, improve body hematopoietic function and blood circulation.And effective immune active ingredient of Chinese herbal medicine can strengthen the generation of animal's antibody, enhancing non-specific immunity, promotes macrophage phagocytic function and lymphocytic conversion ratio, is conducive to the resistant mutation of anti-bacteria.
The present invention's plan, according to the genesis mechanism of Chinese veterinarian's medicine theory, Chinese herbal medicine characteristic and poultry gastroenteritis diarrhea syndrome and poultry digestive system feature, provides compound Chinese medicine extract of a kind of prevention and therapy poultry gastroenteritis diarrhea syndrome and preparation method thereof.Meet the industry requirement of the green cultivation that society is advocated, have vast potential for future development.
Summary of the invention
For the deficiencies in the prior art, the present invention aims to provide and a kind ofly prevents and treats compound Chinese medicine extract of livestock and poultry diarrhea syndrome and its preparation method and application, present inventor is guidance according to Traditional Chinese medical theory, under a large amount of clinical trials, from numerous Chinese crude drug, select suitable medical material and proportioning thereof one by one and formulate the extracting method of its patent medicine, reach the clinical cure rate of 95% and the preventive effect of 100%, and make it be useful in apply clinically.
In order to achieve the above object, the present invention is by the following technical solutions:
Prevent and treat a compound Chinese medicine extract for livestock and poultry diarrhea syndrome, be prepared from by the Chinese crude drug of following weight:
Radix Codonopsis 10-20 part (root is used as medicine),
Rhizoma Atractylodis Macrocephalae 10-20 part (root is used as medicine),
Cortex Phellodendri 10-20 part,
Radix Pulsatillae 10-20 part (all herbal medicine),
Herba Taraxaci 10-20 part (all herbal medicine),
Fructus Chebulae 10-20 part,
Semen Myristicae 10-20 part,
Herba Andrographis 8-16 part,
Herba Portulacae 8-16 part,
Pericarpium Citri Reticulatae 8-16 part,
Poria 8-16 part.
Best, described compound Chinese medicine extract is prepared from by the Chinese crude drug of following weight: Radix Codonopsis 15 parts, the Rhizoma Atractylodis Macrocephalae 15 parts, Cortex Phellodendri 20 parts, the Radix Pulsatillae 20 parts, Herba Taraxaci 15 parts, Fructus Chebulae 10 parts, Semen Myristicae 15 parts, Herba Andrographis 10 parts, Herba Portulacae 10 parts, Pericarpium Citri Reticulatae 10 parts, Poria 10 parts.
The preparation method step of described compound Chinese medicine extract is as follows:
Take each Chinese crude drug of described weight portion, mixing, add each Chinese crude drug gross weight 2-3 water soaking of 60-65 DEG C doubly after 1 hour, feed liquid, in 92 DEG C of reflux, extract, 10 hours, then concentrates, when feed liquid is concentrated into 3 times of each Chinese crude drug gross weight by the water adding each Chinese crude drug gross weight 12 times again, leave standstill 12h, make precipitation, get supernatant and be evaporated to relative density 1.15 ~ 1.20 (55-60 DEG C), spraying dry, receive powder, pulverize, cross 50 mesh sieves, mixing, to obtain final product.
Livestock and poultry diarrhea is mainly caused by endogenous cause of ill and exopathogenic factor, and endogenous cause of ill is transporting and transforming function of the spleen and stomach mistake department mainly, small intestinal clear QI being mixed up with turbid QI; Exopathogenic factor mainly antibacterial, virus, hot and cold etc.; This prescription has effect of heat-clearing and toxic substances removing, removing dampness dysentery relieving, relieving dysentery with astringents, anti-inflammatory analgetic, nourishing complement, makes livestock and poultry diarrhea be able to treating both the principal and secondary aspects of a disease, strengthening vital QI to eliminate pathogenic factors, across-the-board regulation, Yin Yang balancing.This prescription is made Chinese medicine extract simultaneously, by drinking water administration, facilitate and be convenient to absorb, the object for the treatment of can be played rapidly, cure rate reaches more than 95%, and especially poultry reproductive tract are short to efficiently solve poultry physiological feature, and centering drug fiber element is difficult to the problem of absorption and digestion.
The present invention is guidance according to the genesis mechanism of chinese tradition Chinese veterinarian medicine theory, China's " rarity " Chinese herbal medicine characteristic and livestock and poultry diarrhea syndrome and poultry digestive system feature, according to the rigorous formula such as monarch, seven emotions compatibility of Chinese medicine, make it divide the work clearly, Each performs its own functions; Compound Chinese medicine extract of a kind of prevention and therapy livestock and poultry diarrhea syndrome simultaneously screened one by one in a large amount of clinical trial and preparation method thereof.Pharmacological action of the present invention: Radix Codonopsis invigorating the spleen and replenishing QI in this prescription, effect of spleen invigorating lung benefiting, for deficiency of the spleen and lung, anorexia and loose stool; Rhizoma Atractylodis Macrocephalae invigorating the spleen and benefiting QI, dampness diuretic, hidroschesis, for insufficiency of the spleen lack of appetite, abdominal distention is had loose bowels, and Cortex Phellodendri, the Radix Pulsatillae, Herba Taraxaci, Herba Andrographis have heat-clearing and toxic substances removing, effect of antiinflammatory eliminating pathogenic heat from blood to cure dysentery; Fructus Chebulae's refreshing and detoxicating Chinese medicine, cures mainly chronic diarrhea, chronic dysentery, proctoptosis; Semen Myristicae warming middle-JIAO astringing intestine to stop diarrhea; Promote qi circulation digestion promoting, for diarrhea of deficiency type, cold-dysentery, abdominal distention; Herba Portulacae has clearing away heat-damp and promoting diuresis, removing toxic substances and promoting subsidence of swelling, antiinflammatory, quenches the thirst, diuresis; Pericarpium Citri Reticulatae is regulated the flow of vital energy appetizing drying dampness to eliminate phlegm; The function of Poria promoting diuresis to eliminate damp pathogen, strengthening the spleen stomach function regulating, mind tranquilizing and the heart calming.All medicines coordinate the effect with clearing away heat to cure dysentery, invigorating the spleen and benefiting QI, removing heat from blood, stomach invigorating, removing toxic substances, antiinflammatory.
The advantage of technical solution of the present invention is:
1, pure Chinese medicine extraction preparation, nontoxic, and to poultry internal organs not damaged, drug residue free, has no drug resistance, and adapts to outlet poultry most and use;
2, medicine is made Chinese medicine extract, easy to use, efficiently solve the problem that poultry centering drug fiber element is difficult to absorption and digestion, easily absorb, and absorb fast, can play rapidly the object for the treatment of, curative effect time is long;
3, prevention and cure rate high, clinical trial reaches the cure rate of 95% and the preventive effect of 100%.
Detailed description of the invention
In order to make those skilled in the art understand better and implement the present invention, further illustrate the present invention below in conjunction with specific embodiment.But following content should not be understood to the restriction to claims of the present invention request protection domain in any degree.
In the raw materials used Chinese crude drug of following embodiment, Radix Codonopsis originates from Shanxi, and the Rhizoma Atractylodis Macrocephalae originates from Zhejiang, and Cortex Phellodendri originates from Sichuan, the Radix Pulsatillae originates from Guangdong, Herba Taraxaci originates from Hubei, and Fructus Chebulae originates from Yunnan, and Semen Myristicae originates from Guangdong, Herba Andrographis originates from Guangdong, Herba Portulacae originates from Sichuan, and Pericarpium Citri Reticulatae originates from Sichuan, and Poria originates from Dabie Mountains Region.
Raw materials of traditional Chinese medicinal materials is common commercial goods, and wherein Radix Codonopsis and the Rhizoma Atractylodis Macrocephalae are that root is used as medicine, and the Radix Pulsatillae and Herba Taraxaci are all herbal medicines.
Embodiment 1
A compound Chinese medicine extract for prevention and therapy livestock and poultry diarrhea syndrome, is prepared from by the Chinese crude drug of following weight:
Radix Codonopsis 10 parts, the Rhizoma Atractylodis Macrocephalae 10 parts, Cortex Phellodendri 10 parts, the Radix Pulsatillae 10 parts, Herba Taraxaci 10 parts, Fructus Chebulae 10 parts, Semen Myristicae 10 parts, Herba Andrographis 8 parts, Herba Portulacae 8 parts, Pericarpium Citri Reticulatae 8 parts, Poria 8 parts.
The preparation method step of described compound Chinese medicine extract is as follows:
The Chinese crude drug of above-mentioned weight is mixed, the warm water (60-65 DEG C) adding medical material gross weight 2.5 times soaks after 1 hour, the water adding medical material gross weight 12 times again 92 DEG C of heating and refluxing extraction 10 hours in the concentrated unit of extraction, then feed liquid is concentrated, when feed liquid concentrates 3 times of most medical material gross weight, feed liquid vacuum pump is evacuated to settling tank, leave standstill 12h, make precipitation, getting supernatant, to be evaporated to relative density be 1.15 ~ 1.20 (55-60 DEG C), spraying dry, receive powder, pulverize, 50 orders sieve mixing, to obtain final product.
Embodiment 2
A compound Chinese medicine extract for prevention and therapy livestock and poultry diarrhea syndrome, is prepared from by the Chinese crude drug of following weight:
Radix Codonopsis 15 parts, the Rhizoma Atractylodis Macrocephalae 15 parts, Cortex Phellodendri 20 parts, the Radix Pulsatillae 20 parts, Herba Taraxaci 15 parts, Fructus Chebulae 10 parts, Semen Myristicae 15 parts, Herba Andrographis 10 parts, Herba Portulacae 10 parts, Pericarpium Citri Reticulatae 10 parts, Poria 10 parts.Compound Chinese medicine extract is prepared by the preparation method of embodiment 1.
Embodiment 3
A compound Chinese medicine extract for prevention and therapy livestock and poultry diarrhea syndrome, is prepared from by the Chinese crude drug of following weight:
Radix Codonopsis 10 parts, the Rhizoma Atractylodis Macrocephalae 15 parts, Cortex Phellodendri 17 parts, the Radix Pulsatillae 10 parts, Herba Taraxaci 20 parts, Fructus Chebulae 15 parts, Semen Myristicae 10 parts, Herba Andrographis 12 parts, Herba Portulacae 10 parts, Pericarpium Citri Reticulatae 12 parts, Poria 10 parts.Compound Chinese medicine extract is prepared by the preparation method of embodiment 1.
Embodiment 4
A compound Chinese medicine extract for prevention and therapy livestock and poultry diarrhea syndrome, is prepared from by the Chinese crude drug of following weight:
Radix Codonopsis 20 parts, the Rhizoma Atractylodis Macrocephalae 20 parts, Cortex Phellodendri 20 parts, the Radix Pulsatillae 20 parts, Herba Taraxaci 20 parts, Fructus Chebulae 20 parts, Semen Myristicae 20 parts, Herba Andrographis 16 parts, Herba Portulacae 16 parts, Pericarpium Citri Reticulatae 16 parts, Poria 16 parts.Compound Chinese medicine extract is prepared by the preparation method of embodiment 1.
The compound Chinese medicine extract of embodiment 1-4 gained is carried out clinical treatment contrast test, to determine each medical material optimum weight proportioning
Experimental animal: in December, 2012, there is symptom of diarrhea and have a small amount of death in pig farm, Jiangxia District 30, Wuhan, Hubei age in days nursery house 200 piglets 95%, once uses the Drug therapy 4d such as sulfanilamide, gentamycin, enrofloxacin, doxycycline, florfenicol without positive effect;
Testing program: pick out the piglet 160 wherein having obvious symptom of diarrhea on the 5th day, be divided into 4 groups at random; Adopt the compound Chinese medicine extract that embodiment 1-4 is obtained respectively, with 0.2g/kg body weight drinking water administration, every day 3 times;
Result of the test: occur taking a turn for the better the 3rd day most of piglet of continuous drinking water administration, cure rate reaches more than 90%, does not occur cad pig, residual pig etc., and effective percentage reaches 100%, and result of the test is in table 1:
Table 1 four kinds of Chinese medicine extract are to the test of pesticide effectiveness of piglet diarrhea syndrome
This result of the test shows, the cure rate of product to diarrhoea piglet of the present invention's four embodiments is all higher, and the cure rate of the compound Chinese medicine extract that embodiment 2 prescription prepares is the highest, and preventive effect is best, therefore determines that embodiment 2 is best prescription proportioning.
Checking is further illustrated below in conjunction with clinical therapeutic efficacy
Test 1
Experimental animal: in JIUYUE, 2012, age in days 5000 laying hens in laying hen field 210, District, Xinyang Area, Henan Province Pingqiao District occur suffer from diarrhoea and occur death, once 4d was treated without positive effect with neomycin, ofloxacin, florfenicol, death toll rises to 27/d from 7/d, dead 131 altogether, medicine of the present invention within 5th day, is adopted to carry out therapeutic test to morbidity chicken group, the chicken that do not fall ill is mined massively and carries out prophylactic tria with medicine of the present invention, choose 100 laying hens that do not fall ill simultaneously, do not do any treatment process, keep controlled trial group.
Remedy measures: the compound Chinese medicine extract using the embodiment of the present invention 2 to prepare to the chicken group that diarrhoea occurs, with 0.3g/kg body weight drinking water administration, does not fall ill chicken group too with 0.3g/kg body weight drinking water administration, every day 3 times.Within 5th day, stop dead after the treatment of application medicine of the present invention drinking-water, cure rate reaches 95.2%, has obvious therapeutic effect; There is not disease symptom in prevention group; Matched group is all fallen ill, and occurs symptom of diarrhea, and the later stage takes Drug therapy.In addition, when the compound Chinese medicine extract prepared by embodiment 2 is tested, chicken group does not find obvious adverse reaction, and this test shows that the Chinese medicine extract treatment livestock and poultry diarrhea syndrome cure rate prepared according to prescription and the proportioning of embodiment 2 is high, and preventive effect is good.Successive administration treats the result of the test of the 7th day in table 2:
Table 2 Chinese medicine extract is to the test of pesticide effectiveness of laying hen diarrhea syndrome
Test 2
Experimental animal: in December, 2012, age in days nursery house 1000 piglets in pig farm, Jiangxia District 30, Wuhan, Hubei occur suffer from diarrhoea and occur death, once sulfanilamide was used, gentamycin, enrofloxacin, doxycycline, the Drug therapy 5d such as florfenicol are without positive effect, fall ill and occur death after 3 days, 43/d is risen to from 10/d, dead 156 altogether, the compound Chinese medicine extract adopting the embodiment of the present invention 2 to prepare on the 7th day carries out therapeutic test to morbidity piglet, prophylactic tria is carried out to the compound Chinese medicine extract that the piglet that do not fall ill adopts the embodiment of the present invention 2 to prepare, choose 100 piglets that do not fall ill simultaneously, do not do any treatment process, keep controlled trial group.
Remedy measures: the compound Chinese medicine extract using the embodiment of the present invention 2 to prepare to the piglet that diarrhoea occurs, with 0.2g/kg body weight drinking water administration, does not fall ill piglet too with 0.2g/kg body weight drinking water administration, every day 3 times.Within 4th day, stop dead after compound Chinese medicine extract drinking-water treatment prepared by the application embodiment of the present invention 2, cure rate reaches 95.0%, has obvious therapeutic effect; There is not disease symptom in prevention group; Matched group is all fallen ill, and occurs symptom of diarrhea, and the later stage takes Drug therapy.This test shows that the compound Chinese medicine extract treatment livestock and poultry diarrhea syndrome cure rate prepared according to prescription and the proportioning of embodiment 2 is high, and preventive effect is good.