JP2006333845A - Compounding agent for fodder - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a compounding agent for fodder comprising safe ingredients derived from natural sources, more effectively improving healthy conditions of animals through regulation of intestinal environment and promoting their growth, and a fodder compounded with the same. <P>SOLUTION: The compounding agent for fodder contains a vegetable drug and a viable cell agent as effective ingredients. Preferably, the vegetable drug is one or more species selected from a group consisting of Lycii folium, malt, Eriobotryae folium, Panax ginseng, Zizyphi fructus, Dioscoreae rhizoma, Polygonatum sibiricum Red, Lonicerae flos, Kimihide gama, Japanese pumpkin seeds, Plantago seeds, persimmon leaves, leaves of Eucommia ulmoides Oliv., Gardenia jasminoides ellis, Echinacea and common chickweed. Preferably, the viable cell agent is a lactic acid-producing bacillus. The lactic acid-producing bacillus is Lactobacillus sporogenes. <P>COPYRIGHT: (C)2007,JPO&INPIT

Description

  The present invention relates to a feed formulation for animal feed.

  Livestock such as chickens, pigs, sheep and cows are known to cause diarrhea when infected with harmful bacteria such as Escherichia coli or Salmonella or pathogenic viruses during childhood. If these livestock cause diarrhea, they will not be able to absorb enough nutrients from the intestinal tract and growth will stagnate, and their physical strength and resistance will decrease, making it more susceptible to other infections. is there. In addition, fish and shellfish such as aquaculture fish also have a problem that if they are infected with pathogenic microorganisms such as bacteria and viruses, their growth stagnate and the death rate increases.

  On the other hand, in the recent livestock industry, efforts have been made to increase the productivity of livestock and obtain higher economic effects. For example, it has been attempted to increase productivity by increasing the number of captive animals per area, but conversely, stress due to overcrowded breeding is said to reduce the physical strength and resistance of livestock and adversely affect productivity. . Furthermore, once an infection occurs in an overcrowded barn, it spreads throughout the barn and further deteriorates productivity. On the other hand, antibiotics and antibacterial drugs have been mixed with feed for the purpose of preventing disease and promoting growth in livestock. The problem of the safety of livestock products has been pointed out, and there is a demand for safe materials to replace antibiotics and other drugs.

In order to meet such a demand, additives derived from natural products have been proposed. Specifically, feeds for improving meat quality and promoting growth of pigs containing koji leaves, malt, koji leaves, carrots, daikon, mountain medicines, yellow spirits, gold and silver flowers, koji kinko, or chickweed are known. (For example, refer to Patent Document 1). Moreover, the feed additive for livestock which consists of sugar flavor, licorice, and a stevia extract for the purpose of the palatability improvement and diarrhea reduction is known (for example, refer patent document 2). Furthermore, a feed additive for pigs containing nine kinds of herbs, yeast, lactic acid bacteria, and organic acids is known (see, for example, Patent Document 3). Or the feed additive for animals which consists of an herb, a yeast cell wall, a living fungus, and an organic acid is known (for example, refer to patent documents 4).
JP 2004-222547 A JP 2001-95502 A JP 2004-49174 A JP 2002-58434 A

  However, the conventional technique has not been fully effective.

  The present invention is composed of a safe ingredient derived from a natural product, and more effectively prepares an intestinal environment to improve the health condition of livestock and further promotes growth, and a feed compounded therewith. It is intended to provide.

  As a result of intensive investigations to solve such problems, the present inventors have provided an animal with a feed containing a crude drug and a live fungus, and the gut microbiota is improved and diarrhea is prevented. We found that the effects of regulating the intestine and the effect of improving health are dramatically increased, and when these feeds are given to livestock, the growth promotion effect is increased and the productivity of livestock is increased. Has been found to improve, leading to the present invention.

  That is, the first aspect of the present invention is a feed compounding agent characterized by comprising a crude drug and a viable fungus as active ingredients, and preferably the crude drug is koji leaf, malt, koji leaf, One or more herbal medicines selected from the group consisting of carrots, daikon, yamayaku, yellow spirit, gold and silver flowers, kankoei, nanko, car maiko, kashiwanoha, tsunakanoha, yamanakako, echinacare, and chickweed Preferably, the viable agent is a lactic acid-producing bacterium, and more preferably, the lactic acid-producing bacterium is a spore-forming lactic acid bacterium.

  The second aspect of the present invention is an animal feed characterized by blending the above-described feed compounding agent.

  According to the present invention, a safe component derived from a natural product can sufficiently exhibit the effects of improving the intestinal environment and improving the health condition of animals, further promoting growth, It is particularly effective when applied to early livestock and poultry such as calves, piglets and young chicks.

  The present invention will be described in detail below. First, the crude drug used in the present invention will be described. A bamboo leaf is a dried leaf of the solanaceae Solanaceae 枸杞 Lycium ch-inense Miller (wolfberry). Malt is a dried seed of germinated Gramine-ae barley Hordeum vulgare Linne var. Vulgare (barley). The leaves are dried leaves of Rosaceae 枇杷 Eriobotrya japonica L-indley. In addition, ginseng refers to dried roots of Araliaceae ginseng Panax ginseng C.A.Meyer (Ginseng). Oiso refers to the dried fruit of Rhamnaceae 棗 Zizyphus jujuba Miller var. Ine-rmis Rehder (jujube). The mountain pills are those obtained by drying the rhizomes (roots) excluding pericytes of Dioscoreaceae 薯 蕷 Dioscorea japonica Thunberg (Yamanoimo) or Dioscorea batatasDecaisne (Nagaimo). Gold and silver flowers are the dried flowers of Lonicera japonica Thunb-erg. Further, the yellow spirit is a steamed rhizome of the liliaceae Liliaceae yellow spirit Polygonatum falcatum A.Gray (Narukoyuri) Polygonat-um sibiricum Redoute.蒲公英 is a dried root of the compositae Compositae Taraxacum officinale Weber. Nanko is a seed of pumpkin from the Cucurbitaceae family. The forebody is the seed of Plantagoinia Planta asiatica Linne. A bamboo leaf is a leaf of Ebenaceae cynomolgus Diospyros kaki, and a bamboo leaf is a leaf of a eucommia tree. Mountain lion is the fruit of Gardenia jasminoides Ellis of Rubiaceae, and Echinacea is a dried root or rhizome of perennials of the family Asteraceae, Echinacea angustifolia, Echinacea purpura, Echinacea pallida, etc. Jacobe is a dry ground part of Stella-ria neglecta Weihe.

  About these crude drugs, a commercially available thing can be used conveniently. These herbal medicines may be cut into a size of several mm to 1 cm, or may be used after being pulverized to a size of several mm or less.

  Among these herbal medicines, a combination of herbal medicines selected from cocoon leaves, malt, cocoon leaves, carrots, daikon, mountain medicines, yellow spirits, cocoon flowers, gold and silver flowers, echina care, and chickweed are particularly preferable. The mixing ratio in that case is, for example, a combination of 5-30 parts by weight of cocoon leaves, 5-30 parts by weight of malt, 5-30 parts by weight of carrots, 5-30 parts by weight of oak, 5-30 parts by weight of Echinacare Or the combination of 5-30 mass parts of carrots, 5-30 mass parts of large potatoes, 5-30 mass parts of mountain pills, 5-30 mass parts of Jin Ying, and 5-30 mass parts of coconuts is mentioned.

  Next, the viable agent used in the present invention will be described. A viable bacterial agent is a useful intestinal microorganism that can be maintained in a favorable state by ingesting it as animal feed and allowing it to grow in the intestine of the animal. For example, Lactobacillus genus such as Lactobacillus acidophilus, Lactobacillus salvarius, Lactobacillus casei, Bifidobacterium thermophilum, Bifidobacterium pseudolongum, Bifidobacterium longum, etc. Bifidobacterium genus, Enterococcus faecalis, Enterococcus genus such as Enterococcus, Streptococcus thermophilus, Streptococcus mutans Which Streptococcus genus, Pediococcoc stock Genus, Lactococcus, etc., live bacteria commonly called lactic acid bacteria, or Clostridium butyrate Butyric acid bacteria such as cams (Clostridium genus), Bacillus subtilis (Bacillus subtilis), Bacillus cereus, Bacillus badius, Bacillus genus such as Bacillus coagulans, etc. Can be used.

  Among these viable bacteria agents, those that are lactic acid bacteria such as Lactobacillus are preferable, and Bacillus coagulans known as spore-forming lactic acid bacteria can be particularly preferably used. Spore-forming lactic acid bacteria have heat- and acid-resistant properties when present in the form of spores, so there is little deterioration due to heat during the production process of the feed or due to gastric acid in the livestock. It is possible to exert a higher effect within.

The viable cell content in the feed formulation may be about 1.0 × 10 ^{4 to} 1.0 × 10 ¹² cells / g, 1.0 × 10 ^{5 to} 1.0 × 10 ¹⁰ cells / g. g is preferable, and 1.0 × 10 ^{6 to} 1.0 × 10 ⁹ cells / g is more preferable.

  The feed formulation of the present invention is obtained by mixing a predetermined amount of the above components. Any mixing method may be adopted as a mixing method.

As content of the crude drug and the viable fungus contained in 1 g of the feed formulation of the present invention, the crude drug is preferably 0.005 to 0.99 g, more preferably 0.08 to 0.9 g, and 0.1 to 0 0.5 g is most preferred. The viable agent is preferably 1.0 × 10 ^{5 to} 1.0 × 10 ¹⁰ cells as the number of cells.

  The feed additive of the present invention includes mannose and manno-oligosaccharides for the purpose of helping the function of viable fungi, as well as other oligosaccharides such as prebiotics, organic acids, water-soluble dietary fiber, starch, insoluble dietary fiber, etc. May be added. Furthermore, a stabilizer and an excipient may be included as necessary. Examples of the stabilizer include silicic acid anhydride, and examples of the excipient include bran, wheat flour, corn starch, corn gluten feed, dextrin, rice bran and the like.

  The 2nd animal feed of this invention mix | blends said feed compounding agent. The blending ratio is preferably 0.005 to 5% by weight, more preferably 0.02 to 2% by weight, and most preferably 0.1 to 2% by weight.

  Hereinafter, the present invention will be described specifically by way of examples. In addition, this invention is not limited to an Example.

Example 1
20 parts by weight of maple leaves, 20 parts by weight of malt, 15 parts by weight of mountain medicine, 10 parts by weight of carrots, 10 parts by weight of carrots, 5 parts by weight of maple leaves, 5 parts by weight of yellow spirits, 5 parts by weight of gold and silver flowers, 5 parts by weight of Koei Yin And 0.4 g of herbal medicine mixed at a ratio of 5 parts by weight of chickweed, 2 × 10 ⁸ cells (equivalent to 0.2 g) of spore-forming lactic acid bacteria Bacillus coagulans (trade name “Lacris-10”, Sankyo Lifetech), 0.4 g of wheat flour was mixed to prepare the feed formulation of the present invention.

Example 2
20 parts by weight of maple leaves, 20 parts by weight of malt, 15 parts by weight of mountain medicine, 10 parts by weight of carrots, 10 parts by weight of carrots, 5 parts by weight of maple leaves, 5 parts by weight of yellow spirits, 5 parts by weight of gold and silver flowers, 5 parts by weight of Koei Yin And 0.4 g of herbal medicine mixed at a ratio of 5 parts by weight of the herb and a live fungus for feed (B. thermophilus, E. fesium, L. acidophilus combination, trade name “LEB mix”, Morinaga Milk Products ) 4 × 10 ⁹ cells (equivalent to 0.2 g) and 0.4 g of wheat flour were mixed to prepare the feed formulation of the present invention.

Example 3
Mixed in proportions of 20 parts by weight of kashiwa, 20 parts by mass of malt, 15 parts by mass of Kunihide Kashiwa, 15 parts by mass of yam, 10 parts by mass of ginseng, 10 parts by mass of ginseng, 5 parts by mass of Echinacare, and 5 parts by mass of kakinoha The feed of the present invention is prepared by mixing 0.4 g of crude drug, 2 × 10 ⁸ cells (equivalent to 0.2 g) of spore-forming lactic acid bacteria Bacillus coagulance (trade name “Lacris-10”, manufactured by Sankyo Lifetech) and 0.4 g of flour. A compounding agent was prepared.

Example 4
Mixed in proportions of 20 parts by weight of kashiwa, 20 parts by mass of malt, 15 parts by mass of Kunihide Kashiwa, 15 parts by mass of yam, 10 parts by mass of ginseng, 10 parts by mass of ginseng, 5 parts by mass of Echinacare, and 5 parts by mass of kakinoha 0.4 g of crude drug and live fungus for feed (B. thermophilus, E. fesium, L. acidophilus combination, trade name “LEB mix”, Morinaga Milk Industry) 4 × 10 ⁹ cells (equivalent to 0.4 g) ) And 0.4 g of wheat flour were mixed to prepare the feed formulation of the present invention.

Comparative Example 1
20 parts by weight of maple leaves, 20 parts by weight of malt, 15 parts by weight of mountain medicine, 10 parts by weight of carrots, 10 parts by weight of carrots, 5 parts by weight of maple leaves, 5 parts by weight of yellow spirits, 5 parts by weight of gold and silver flowers, 5 parts by weight of Koei Yin And the crude drug 0.4g and the wheat flour 0.6g which were mixed in the ratio of 5 parts by weight of the chickweed were mixed to prepare a feed formulation.

Comparative Example 2
Mixed in proportions of 20 parts by weight of kashiwa, 20 parts by mass of malt, 15 parts by mass of Kunihide Kashiwa, 15 parts by mass of yam, 10 parts by mass of ginseng, 10 parts by mass of ginseng, 5 parts by mass of Echinacare, and 5 parts by mass of kakinoha Crude drug 0.4 g and wheat flour 0.6 g were mixed to prepare a feed formulation.

Comparative Example 3
A spore-forming lactic acid bacterium Bacillus coagulance (trade name “Lacris-10”, manufactured by Sankyo Lifetech) 2 × 10 ⁸ cells (corresponding to 0.2 g) and 0.8 g of wheat flour were mixed to prepare a feed formulation.

Comparative Example 4
Live fungus for feed (B. thermophilus, E. fesium, L. acidophilus combination, trade name “LEB mix”, Morinaga Milk Industry Co., Ltd.) 4 × 10 ⁹ cells (equivalent to 0.4 g) and wheat flour 0. 8g was mixed and the feed formulation was produced.

Test example 1
The feed formulation prepared in Examples 1 to 4 and Comparative Examples 1 to 4 was added to commercially available artificial milk for piglets to prepare a blended feed. The feed compounding rate was 0.3%, and the artificial milk for piglets was changed according to the growth stage of the piglets. A group of 30 piglets were fed the prepared feed and reared from 5 to 70 days of age. Moreover, as a comparison, only the commercially available artificial milk for piglets was fed without adding the feed compounding agent and reared in the same manner (Comparative Example 5).

  The stool state from 5 days to 40 days of age during the breeding period of the domestic pigs was observed.

As a result of the above, compared with the piglets bred with the feed formulation prepared in the comparative example, the number of days of diarrhea occurring in the piglets bred with the feed formulation prepared in the example, that is, herbal medicine It was shown that the effect of preventing diarrhea in piglets is increased synergistically when combined with the microbial agent.

  In addition, Table 2 shows the results of collecting the feces at 30 days of age of the piglets raised in Test Example 1 and examining the bacterial flora.

Based on the above results, Bifidobacterium and Lactobacillus bacteria, which are generally referred to as useful intestinal microorganisms, are feed formulations prepared in the Examples rather than the piglets fed with the feed formulation prepared in the Comparative Example. More piglets were fed and fed. On the other hand, Salmonella, which is a bad bacterium, was not detected in the feces of piglets fed with the feed formulation prepared in the test example, whereas Escherichia coli was detected only slightly, whereas in the comparative example Many were detected from the feces of piglets fed with the prepared feed formulation. That is, it was recognized that the feed formulation combining a herb medicine and a fungus agent has a high intestinal regulating action for adjusting the intestinal microflora of the piglet.

  Table 3 shows the results of visual observation of the health condition of the piglets raised in Test 1 during the breeding period.

From the above results, compared to the comparative example, the piglet bred with the feed formulation prepared in the example is more vigorous and active, has good skin tone, and the stomach tension is also good. It was good and overall health was found to be good.

  Furthermore, Table 4 shows the results of comparing the average body weights at birth, 21 days of age, and 70 days of age during the breeding period of the pigs raised in Test Example 1.

From the above results, compared to the comparative example, the piglets bred with the feed formulation prepared in the Examples were larger in both 21-day-old and 70-day-old weights, that is, a combination of herbal medicine and viable fungus. In some cases, the growth promotion effect of piglets was shown to increase synergistically.

Test example 2
The feed formulation prepared in Examples 1 and 3 and Comparative Examples 1 and 3 was added to a commercial broiler feed to prepare a blended feed. The addition rate of the feed formulation was 0.2%, and the feed was changed according to the growth stage of the chicken. A group of 50 broiler chicks were fed with feed and fed from feeding to shipping (53 days of age). In addition, as a comparison, only commercially available broiler feed was fed without adding the feed formulation, and the animals were reared in the same manner (Comparative Example 6).

  Table 5 shows the weight of the chicks bred in Test 2 and the feed demand rate at the time of shipment.

From the above results, broilers fed with a feed formulation consisting of the crude drug and the live fungus according to the present invention are compared to the feed formulation prepared in the comparative example, or the case of breeding with only commercially available feed, And the feed demand rate was improved.

As a result of the above, feeding the animal with the feed formulation of the present invention, combined with the effects of preventing the diarrhea of the animal and improving the intestinal regulation and health by improving the increase of intestinal bacteria, promotes growth promotion. It turns out that it raises synergistically.

Claims (5)

A feed formulation comprising a crude drug and a fungus agent as active ingredients. Herbal medicine is from the group consisting of oak, malt, oak, carrot, daikon, yamayaku, yellow spirit, gold and silver flowers, koei ei, nanko, car maiko, kashiwa, yunaka, yamako, echina care and chickweed The feed formulation according to claim 1, which is one or more selected herbal medicines. The feed formulation according to claim 1 or 2, wherein the viable agent is a lactic acid-producing bacterium. The feed formulation according to claim 3, wherein the lactic acid-producing bacterium is a sporic lactic acid bacterium. An animal feed comprising the feed formulation according to any one of claims 1 to 4.