CN117481255A - Anti-stress growth-promoting preparation for livestock and poultry and application of anti-stress growth-promoting preparation in aspect of improving feed utilization rate - Google Patents
Anti-stress growth-promoting preparation for livestock and poultry and application of anti-stress growth-promoting preparation in aspect of improving feed utilization rate Download PDFInfo
- Publication number
- CN117481255A CN117481255A CN202311658346.6A CN202311658346A CN117481255A CN 117481255 A CN117481255 A CN 117481255A CN 202311658346 A CN202311658346 A CN 202311658346A CN 117481255 A CN117481255 A CN 117481255A
- Authority
- CN
- China
- Prior art keywords
- parts
- livestock
- poultry
- stress
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 244000144972 livestock Species 0.000 title claims abstract description 40
- 244000144977 poultry Species 0.000 title claims abstract description 38
- 238000002360 preparation method Methods 0.000 title claims abstract description 32
- 230000002180 anti-stress Effects 0.000 title abstract description 11
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 23
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 23
- 241000193171 Clostridium butyricum Species 0.000 claims abstract description 23
- 241000194032 Enterococcus faecalis Species 0.000 claims abstract description 21
- 229940032049 enterococcus faecalis Drugs 0.000 claims abstract description 21
- UFVBOGYDCJNLPM-UHFFFAOYSA-L disodium;9-carboxy-4,5-dioxo-1h-pyrrolo[2,3-f]quinoline-2,7-dicarboxylate Chemical compound [Na+].[Na+].C12=C(C([O-])=O)C=C(C([O-])=O)N=C2C(=O)C(=O)C2=C1NC(C(=O)O)=C2 UFVBOGYDCJNLPM-UHFFFAOYSA-L 0.000 claims abstract description 18
- 239000002994 raw material Substances 0.000 claims abstract description 5
- 230000003078 antioxidant effect Effects 0.000 claims description 17
- 239000003963 antioxidant agent Substances 0.000 claims description 11
- 238000004321 preservation Methods 0.000 claims description 10
- 230000000694 effects Effects 0.000 abstract description 15
- 230000001737 promoting effect Effects 0.000 abstract description 13
- 230000036542 oxidative stress Effects 0.000 abstract description 10
- 239000003674 animal food additive Substances 0.000 abstract description 4
- 230000002195 synergetic effect Effects 0.000 abstract description 2
- 150000001875 compounds Chemical class 0.000 abstract 1
- 235000013594 poultry meat Nutrition 0.000 description 31
- 230000035882 stress Effects 0.000 description 25
- 230000000052 comparative effect Effects 0.000 description 17
- 238000011282 treatment Methods 0.000 description 16
- 241000287828 Gallus gallus Species 0.000 description 15
- 238000012360 testing method Methods 0.000 description 15
- 230000002354 daily effect Effects 0.000 description 11
- 241001465754 Metazoa Species 0.000 description 6
- 230000008901 benefit Effects 0.000 description 6
- 235000013330 chicken meat Nutrition 0.000 description 6
- 238000004519 manufacturing process Methods 0.000 description 6
- 230000003647 oxidation Effects 0.000 description 5
- 238000007254 oxidation reaction Methods 0.000 description 5
- 235000021050 feed intake Nutrition 0.000 description 4
- 210000004185 liver Anatomy 0.000 description 4
- WSMYVTOQOOLQHP-UHFFFAOYSA-N Malondialdehyde Chemical compound O=CCC=O WSMYVTOQOOLQHP-UHFFFAOYSA-N 0.000 description 3
- 230000003064 anti-oxidating effect Effects 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 2
- 241000282887 Suidae Species 0.000 description 2
- 230000037396 body weight Effects 0.000 description 2
- 229960005069 calcium Drugs 0.000 description 2
- 229910052791 calcium Inorganic materials 0.000 description 2
- 239000011575 calcium Substances 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 230000008645 cold stress Effects 0.000 description 2
- 239000006027 corn-soybean meal Substances 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 230000037213 diet Effects 0.000 description 2
- 235000005911 diet Nutrition 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 238000009472 formulation Methods 0.000 description 2
- 230000008642 heat stress Effects 0.000 description 2
- 230000000968 intestinal effect Effects 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 239000013642 negative control Substances 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 239000013641 positive control Substances 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- 208000011580 syndromic disease Diseases 0.000 description 2
- 238000005303 weighing Methods 0.000 description 2
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- FERIUCNNQQJTOY-UHFFFAOYSA-N Butyric acid Natural products CCCC(O)=O FERIUCNNQQJTOY-UHFFFAOYSA-N 0.000 description 1
- 235000019750 Crude protein Nutrition 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000006587 Glutathione peroxidase Human genes 0.000 description 1
- 108700016172 Glutathione peroxidases Proteins 0.000 description 1
- 240000007232 Illicium verum Species 0.000 description 1
- 235000008227 Illicium verum Nutrition 0.000 description 1
- 241000186660 Lactobacillus Species 0.000 description 1
- 231100000678 Mycotoxin Toxicity 0.000 description 1
- 241000235342 Saccharomycetes Species 0.000 description 1
- 102000019197 Superoxide Dismutase Human genes 0.000 description 1
- 108010012715 Superoxide dismutase Proteins 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 150000001408 amides Chemical class 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- -1 butyrate diester Chemical class 0.000 description 1
- MKJXYGKVIBWPFZ-CEOVSRFSSA-L calcium;(2s)-2-hydroxypropanoate Chemical compound [Ca+2].C[C@H](O)C([O-])=O.C[C@H](O)C([O-])=O MKJXYGKVIBWPFZ-CEOVSRFSSA-L 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 210000000750 endocrine system Anatomy 0.000 description 1
- 230000037149 energy metabolism Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 230000036737 immune function Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 210000004347 intestinal mucosa Anatomy 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 229940039696 lactobacillus Drugs 0.000 description 1
- 229940118019 malondialdehyde Drugs 0.000 description 1
- 208000030159 metabolic disease Diseases 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- 239000002636 mycotoxin Substances 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000004792 oxidative damage Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 238000003307 slaughter Methods 0.000 description 1
- 229940045955 star anise extract Drugs 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- KDYFGRWQOYBRFD-UHFFFAOYSA-L succinate(2-) Chemical compound [O-]C(=O)CCC([O-])=O KDYFGRWQOYBRFD-UHFFFAOYSA-L 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/116—Heterocyclic compounds
- A23K20/137—Heterocyclic compounds containing two hetero atoms, of which at least one is nitrogen
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Fodder In General (AREA)
- Feed For Specific Animals (AREA)
Abstract
The invention relates to an anti-stress growth-promoting preparation for livestock and poultry and application thereof in improving the utilization rate of feed, belonging to the technical field of feed additives, wherein the preparation comprises the following raw materials in parts by weight: 1 to 5 parts of pyrroloquinoline quinone disodium salt, 1 to 10 parts of clostridium butyricum, 1 to 5 parts of bacillus subtilis and 1 to 5 parts of enterococcus faecalis. The invention mixes the pyrroloquinoline quinone disodium salt with clostridium butyricum, bacillus subtilis and enterococcus faecalis by using the pyrroloquinoline quinone disodium salt. Compared with the single use of pyrroloquinoline quinone disodium salt or the single use of clostridium butyricum, bacillus subtilis and enterococcus faecalis, the compound has synergistic effect, and has remarkable effects of improving the feed utilization rate and promoting growth while resisting oxidative stress.
Description
Technical Field
The invention belongs to the technical field of feed additives, and particularly relates to an anti-stress growth-promoting preparation for livestock and poultry and application of the preparation in the aspect of improving the feed utilization rate.
Background
In modern livestock and poultry cultivation and production, stress is an unavoidable problem, and there are many stress sources causing livestock and poultry stress, such as weaning stress, transportation stress and the like caused by management factors, and heat stress and cold stress caused by stimulation of environmental factors. A large number of researches show that the stress is closely related to oxidative stress, and cold stress can cause energy metabolism disorder, immune function reduction, immune organ and antioxidation function damage, production performance reduction and even death of livestock and poultry, especially young animals; heat stress can affect the endocrine system of livestock and poultry, so that the organism metabolism is disturbed, the growth performance is reduced, and the reproductive function is damaged; the transportation stress can damage the oxidation resistance and oxidation balance state of animals, and cause oxidative damage of organisms; early weaning has become an important management of improving fertility and growth performance in young animals, but early weaning can lead to weaning stress syndrome in young animals. Weaning stress can trigger oxidative stress, which in turn exacerbates the "early weaning syndrome". In general, under the stress state, the free radicals of the livestock and poultry bodies are excessively generated, the metabolic balance of the bodies is broken, so that the bodies are subjected to oxidative stress, and the oxidative stress is an important cause for reducing the production performance and increasing diseases of the livestock and poultry.
The addition of some drugs or additives to the feed, such as VC, is often referred to as "anti-stress factor" or succinate (butyrate diester). However, these drugs have a single effect and are only resistant to stress. Patent number CN 202210632958.7' A stress-resistant growth-promoting feed additive for livestock and poultry, which is composed of an illicium verum extract, clostridium butyricum and bacillus subtilis, is disclosed, and has the effects of resisting stress and promoting growth, but has the advantages of large consumption of feed and high feed coefficient.
Disclosure of Invention
Aiming at the prior art, the invention aims to provide an anti-stress growth-promoting preparation for livestock and poultry and application of the anti-stress growth-promoting preparation in the aspect of improving the feed utilization rate. The feed additive has the anti-stress effect, can improve the feed conversion rate and promote the growth of livestock.
In order to achieve the above purpose, the invention adopts the following technical scheme:
the invention provides an antioxidant stress growth-promoting preparation for livestock and poultry, which comprises the following raw materials in parts by weight: 1 to 5 parts of pyrroloquinoline quinone disodium salt, 1 to 10 parts of clostridium butyricum, 1 to 5 parts of bacillus subtilis and 1 to 5 parts of enterococcus faecalis.
Preferably, the material comprises the following raw materials in parts by weight: 2 parts of pyrroloquinoline quinone disodium salt, 5 parts of clostridium butyricum, 3 parts of bacillus subtilis and 3 parts of enterococcus faecalis.
Preferably, the clostridium butyricum has a biological accession number of: cgmccno.6731; the preservation number of the bacillus subtilis is CGMCC No.15646; the enterococcus faecalis has the biological preservation number of: cgmccno.19078.
In a second aspect, the invention provides application of the preparation in improving the utilization rate of livestock and poultry feed.
In a third aspect of the invention, a feed added with the preparation for resisting oxidative stress and promoting growth of livestock and poultry is provided, wherein the addition amount of the preparation in the feed for livestock and poultry is 200-250 g/ton.
Compared with the prior art, the invention has the beneficial effects that:
(1) The preparation method of the invention is simple, has low cost and no toxic or side effect, can be used for a long time, can not generate drug resistance for livestock, and can be widely used in various poultry and livestock.
(2) According to the invention, the pyrroloquinoline quinone disodium salt is mixed with clostridium butyricum, bacillus subtilis and enterococcus faecalis, and compared with the pyrroloquinoline quinone disodium salt which is singly used or the clostridium butyricum, bacillus subtilis and enterococcus faecalis which are singly used, the pyrroloquinoline quinone disodium salt has synergistic effect with clostridium butyricum, bacillus subtilis and enterococcus faecalis, and the effects of improving the feed utilization rate and promoting growth are remarkable while resisting oxidative stress.
Detailed Description
It should be noted that the following detailed description is illustrative and is intended to provide further explanation of the present application. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs.
Clostridium butyricum with the preservation number of CGMCCNo.6731 and bacillus subtilis with the preservation number of CGMCCNo.15646 can effectively inhibit the number of harmful bacteria in the intestinal tracts of livestock and poultry and correct the disturbance of intestinal flora. The enterococcus faecalis with the preservation number of CGMCC No.19078 can form a biological film in the intestinal canal of the animal to be adhered to the intestinal mucosa of the animal, develop, grow and reproduce, can form a lactobacillus barrier, resist the side effects of external germ virus, mycotoxin and the like, is not possessed by bacillus and saccharomycetes, and can decompose part of protein into amide and amino acid, convert most of nitrogen-free extract of carbohydrate into L-type lactic acid, react with calcium to synthesize L-calcium lactate and promote the absorption of calcium by livestock and poultry. Enterococcus faecalis can also soften fibers in the feed and improve the conversion rate of the feed. The inventor researches find that the application of the pyrroloquinoline quinone disodium salt, clostridium butyricum, bacillus subtilis and enterococcus faecalis together has the effect of improving the feed utilization rate, can effectively promote the growth of livestock and poultry, reduces the feed cost and improves the economic benefit.
In order to enable those skilled in the art to more clearly understand the technical solutions of the present application, the technical solutions of the present application will be described in detail below with reference to specific embodiments.
The test materials used in the examples of the present invention are all conventional in the art and are commercially available.
Description: the preservation number of clostridium butyricum is CGMCC No.6731; the preservation number of the bacillus subtilis is CGMCC No.15646; enterococcus faecalis with collection number of CGMCC No.19078 is purchased from China general microbiological culture Collection center, and bacillus subtilis is 2.0X10 10 Enterococcus faecalis 2.0X10 9 Clostridium butyricum 5.0X10 8 The method comprises the steps of carrying out a first treatment on the surface of the Pyrroloquinoline quinone disodium salt, available fromShandong Jincheng biological pharmaceutical Co., ltd.
Example 1: and weighing 300g of pyrroloquinoline quinone disodium salt, 300g of clostridium butyricum and 400g of bacillus subtilis according to parts by weight, and uniformly mixing to obtain the anti-oxidative stress growth-promoting preparation for livestock and poultry.
Example 2: 200g of pyrroloquinoline quinone disodium salt, 100g of clostridium butyricum, 200g of enterococcus faecalis and 500g of bacillus subtilis are weighed according to parts by weight and uniformly mixed to obtain the anti-oxidative stress growth-promoting preparation for livestock and poultry.
Example 3: 200g of pyrroloquinoline quinone disodium salt, 500g of clostridium butyricum, 300g of enterococcus faecalis and 300g of bacillus subtilis are weighed according to parts by weight and uniformly mixed to obtain the anti-oxidative stress growth-promoting preparation for livestock and poultry.
Comparative example 1: 200g of pyrroloquinoline quinone disodium salt is used as an antioxidant stress growth promoting preparation for livestock and poultry.
Comparative example 2: 200g clostridium butyricum is used as an antioxidant stress growth promoting preparation for livestock and poultry.
Comparative example 3: 300g of bacillus subtilis is used as an antioxidant stress growth promoting preparation for livestock and poultry.
Comparative example 4: 300g of enterococcus faecalis is used as an antioxidant stress growth promoting preparation for livestock and poultry.
Comparative example 5: 200g of star anise extract, 500g of clostridium butyricum and 300g of bacillus subtilis are weighed according to parts by weight and uniformly mixed to obtain the preparation for resisting oxidative stress and promoting growth of livestock and poultry.
Test example 1
Healthy 1-day-old AA broilers 2160 with substantially consistent body weight were selected and randomly split into 9 treatments, 6 replicates each, and 40 replicates each. Treatment 1: a control group fed with corn-soybean meal base ration; treatments 2 to 9, based on the treatment 1 ration, the antioxidant stress growth promoting agents for livestock and poultry prepared in examples 1 to 3 and comparative examples 1 to 5 were added, and they were designated as examples 1 to 3 and comparative examples 1 to 5. The test was divided into 2 stages (0-21 d,22-42 d). The test chicken is kept in cages, and is fed with granular feed, and the daily ration composition and the nutrition level are consistent. The production performance is monitored in the test period, 6 chickens (1/repetition) are randomly selected in each treatment for testing the chickens 42d, slaughter experiments are carried out, serum and liver samples are taken, and the oxidation resistance is measured.
The production performance is as follows: and recording feed intake every day by taking repetition as a unit, weighing the test chickens at the beginning and the end of the test, and calculating daily gain, daily feed intake and mortality.
Oxidation resistance index: at the end of the test period, 12 chickens (2/replicates) were randomly selected for each treatment, blood was collected and liver samples were taken, and antioxidant indicators (superoxide dismutase activity (SOD), glutathione peroxidase activity (GSH-PX), malondialdehyde content (MDA)) were determined. The results of the growth performance and oxidation resistance during the test period are shown in tables 1 and 2.
TABLE 1 growth Properties
As can be seen from Table 1, with the formulations prepared in examples 1-3, the daily gain of the broiler chickens was higher than that of comparative examples 1-4 and the control group, and the weight of the broiler chickens was lower than that of comparative examples 1-4 and the control group. In particular, the preparation prepared in the embodiment 3 can obviously improve the daily gain of broiler chickens and reduce the feed weight ratio. Similarly, only example 3 gave the highest performance in broiler chicken production as compared to comparative example 5.
TABLE 2 antioxidant Properties
As can be seen from Table 2, the antioxidant stress growth promoting preparation of livestock and poultry prepared in examples 1-3 has better activity of antioxidant enzyme in the serum and liver of broiler chicken than those in comparative examples 1-4 and control group, and the MDA content in liver of examples 1-3 is lower than that in comparative examples 1-4 and control group, so that the effect obtained in example 3 is most remarkable.
As can be seen from tables 1-2, the preparation for resisting oxidative stress and promoting growth of livestock and poultry prepared by the invention is prepared by the antioxidation effect of pyrroloquinoline quinone disodium salt, and by the bacteriostasis effect of clostridium butyricum and bacillus subtilis and the feed utilization rate improving effect of enterococcus faecalis, the antioxidation stress of livestock and poultry is synergistically promoted, and the growth of livestock and poultry is promoted.
Test example 2
Healthy 1 day old commercial AA broilers 1440 with substantially uniform body weight were selected and randomly divided into 4 treatments, 9 replicates per treatment, and 40 chickens per replicate. Treatment 1, positive control group, feeding normal nutritional level diet; treatment 2, negative control group, fed low nutrient level diet (energy reduced by 30kcal/kg, i.e. 0.126MJ/kg, crude protein reduced by 0.5%); treatments 3 to 4, and on the basis of treatments 1 and 2, example 3 was added, and the growth performance and economic benefits were measured at 21 days old and 42 days old, and the test period results are shown in tables 3 and 4.
TABLE 3 growth Properties
| Project | Daily gain g/d | Daily feed intake g/d | Ratio of material to weight | Weight g/g only |
| Positive control group | 64.32 | 90.57 | 1.39 | 2743 |
| Negative control group | 63.06 | 90.71 | 1.42 | 2690 |
| Positive example 3 | 64.58 | 90.63 | 1.39 | 2754 |
| Negative example 3 | 63.72 | 90.21 | 1.40 | 2717 |
TABLE 4 economic benefits
As can be seen from tables 3 to 4, the addition of example 3 to the ration can significantly increase the daily gain and daily feed intake of the broiler chickens, reduce the feed-to-weight ratio and increase the weight of the broiler chickens, as compared with the control group. The economic benefit obtained by adding example 3 at normal nutrient levels is best, and the same economic benefit can be obtained by adding example 3 while reducing nutrient levels.
Test example 3
And selecting 324 healthy 24-day-old three-way hybrid commercial weaned pigs with basically consistent weight, randomly dividing the three-way hybrid commercial weaned pigs into 9 groups, repeating 6 times each group, and performing a 28-day breeding test on 6 times each group. Treatment 1: a control group fed with corn-soybean meal base ration; treatments 2 to 9, in which the anti-stress agents prepared in examples 1 to 3 and comparative examples 1 to 5 were added on the basis of treatment 1 ration, respectively, were designated as examples 1 to 3 and comparative examples 1 to 5. The growth performance was monitored during the test period.
TABLE 5 growth Properties
As is clear from Table 5, the daily gain of piglets was higher than that of the comparative examples 1 to 4 and the control group, and the weight of piglets was lower than that of the comparative examples 1 to 4 and the control group, using the antioxidant stress growth-promoting formulations prepared in examples 1 to 3. In particular, compared with comparative example 5, the preparation for resisting oxidative stress and promoting growth prepared in example 3 can obviously improve daily gain of piglets and reduce the feed-to-weight ratio.
The foregoing description is only of the preferred embodiments of the present application and is not intended to limit the same, but rather, various modifications and variations may be made by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principles of the present application should be included in the protection scope of the present application.
Claims (4)
1. The preparation is characterized by comprising the following raw materials in parts by weight: 1-5 parts of pyrroloquinoline quinone disodium salt, 1-10 parts of clostridium butyricum, 1-5 parts of bacillus subtilis and 1-5 parts of enterococcus faecalis, wherein the biological preservation number of the clostridium butyricum is as follows: cgmccno.6731; the preservation number of the bacillus subtilis is CGMCC No.15646; the enterococcus faecalis has the biological preservation number of: cgmccno.19078.
2. The anti-oxidative stress growth-promoting preparation for livestock and poultry according to claim 1, which is characterized by comprising the following raw materials in parts by weight: 2 parts of pyrroloquinoline quinone disodium salt, 5 parts of clostridium butyricum, 3 parts of bacillus subtilis and 3 parts of enterococcus faecalis.
3. The use of the antioxidant stress growth-promoting preparation for livestock and poultry according to claim 1 or 2 for improving the utilization rate of livestock and poultry feed.
4. A feed added with the anti-oxidative stress growth-promoting preparation of livestock and poultry according to claim 1 or 2, which is characterized in that the addition amount of the preparation in the livestock and poultry feed is 200-250 g/ton.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311658346.6A CN117481255B (en) | 2023-12-06 | 2023-12-06 | Anti-stress growth-promoting preparation for livestock and poultry and application of anti-stress growth-promoting preparation in aspect of improving feed utilization rate |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311658346.6A CN117481255B (en) | 2023-12-06 | 2023-12-06 | Anti-stress growth-promoting preparation for livestock and poultry and application of anti-stress growth-promoting preparation in aspect of improving feed utilization rate |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN117481255A true CN117481255A (en) | 2024-02-02 |
| CN117481255B CN117481255B (en) | 2024-03-19 |
Family
ID=89674654
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202311658346.6A Active CN117481255B (en) | 2023-12-06 | 2023-12-06 | Anti-stress growth-promoting preparation for livestock and poultry and application of anti-stress growth-promoting preparation in aspect of improving feed utilization rate |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN117481255B (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006333845A (en) * | 2005-06-06 | 2006-12-14 | Unitika Ltd | Compounding agent for fodder |
| CN113122466A (en) * | 2019-12-31 | 2021-07-16 | 杭州远大生物制药有限公司 | Enterococcus faecalis and application thereof |
| RU2759225C1 (en) * | 2020-09-09 | 2021-11-11 | Общество с ограниченной ответственностью "БИОТРОФ" | Additive containing a preparation exhibiting probiotic activity and organic acids for feed for agricultural animals and poultry |
| US20220117264A1 (en) * | 2019-01-10 | 2022-04-21 | Evonik Operations Gmbh | Fermentation broths and their use |
| CN115211492A (en) * | 2022-06-07 | 2022-10-21 | 圣道生物技术(山东)有限公司 | Stress relieving medicine for resisting stress and promoting growth of livestock and poultry and preparation method and application thereof |
-
2023
- 2023-12-06 CN CN202311658346.6A patent/CN117481255B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006333845A (en) * | 2005-06-06 | 2006-12-14 | Unitika Ltd | Compounding agent for fodder |
| US20220117264A1 (en) * | 2019-01-10 | 2022-04-21 | Evonik Operations Gmbh | Fermentation broths and their use |
| CN113122466A (en) * | 2019-12-31 | 2021-07-16 | 杭州远大生物制药有限公司 | Enterococcus faecalis and application thereof |
| RU2759225C1 (en) * | 2020-09-09 | 2021-11-11 | Общество с ограниченной ответственностью "БИОТРОФ" | Additive containing a preparation exhibiting probiotic activity and organic acids for feed for agricultural animals and poultry |
| CN115211492A (en) * | 2022-06-07 | 2022-10-21 | 圣道生物技术(山东)有限公司 | Stress relieving medicine for resisting stress and promoting growth of livestock and poultry and preparation method and application thereof |
Non-Patent Citations (1)
| Title |
|---|
| 杨玲;夏嗣廷;胡睿智;欧阳富龙;贺建华;: "丁酸梭菌的功能性研究进展及其在畜禽生产中的应用", 中国畜牧兽医, no. 11, 14 November 2018 (2018-11-14) * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN117481255B (en) | 2024-03-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103250879B (en) | Compound feed for adjusting intestinal development of piglets, and application thereof | |
| BR112016026359B1 (en) | Methods for controlling the harmful environmental effects of manure and for feeding an animal, packaging, feed and drinking water additives, and feed composition | |
| CN101797016B (en) | Animal growth modulating agent and preparation method thereof | |
| WO2022063296A1 (en) | Feed additive for improving oxygen obtaining ability of poultry and use thereof | |
| CN107259186B (en) | Laying hen feed for producing high-calcium high-zinc nutritional eggs and preparation method thereof | |
| CN102885202A (en) | Novel poultry micro-ecological preparation and preparation method thereof | |
| CN111066972A (en) | Feed for improving growth performance of fattening pigs and feeding method | |
| Haryuni et al. | Improving the productivity of Joper chickens with fermented soy milk waste (SMW) | |
| CN112262917A (en) | Preparation method of high-viable bacteria fermented feed | |
| CN110892944A (en) | Weaned pig feed additive capable of replacing antibiotics and application thereof | |
| CN102228141B (en) | Fattening pig feed without bean pulp and calcium hydrophosphate | |
| KR0143954B1 (en) | Composition for enhancing fertility and weight gain in animals and preparations containing same | |
| CN117481255B (en) | Anti-stress growth-promoting preparation for livestock and poultry and application of anti-stress growth-promoting preparation in aspect of improving feed utilization rate | |
| CN114304413B (en) | Composite containing acidifying agent and preparation method thereof | |
| Jordan | Aureomycin supplements in lamb fattening rations | |
| CN111357886A (en) | Premix for laying hen in initial stage, preparation method thereof, complete feed thereof and application thereof | |
| CN108967703A (en) | A kind of green feed additive, premix and mixed feed improving blue pool Meat | |
| CN111134079A (en) | Cultivation technology of selenium-rich beef cattle | |
| CN110179006A (en) | The fowl dirty health care growth-promoting feedstuff additive of drop and its process | |
| RU2809377C1 (en) | Method of using prebiotic lactulose-containing feed additive in diets of poultry for meat and egg production | |
| GÖÇMEN et al. | Effects of magnesium sources and levels on performance, carcass traits and meat quality in broiler | |
| CN113133429B (en) | Method for improving immunity and oxidation resistance of dairy cows | |
| Kang et al. | Effect of dietary supplementation of fermented by-products of garlic and onion on production performance, blood components and cecal microflora in broiler chicks | |
| Jaishankar | Role of feed additives on production performance of Giriraja chicks | |
| Gül et al. | EFFECT OF USING PROBIOTICS SUPPLEMENTED WHEAT INSTEAD OF CORN IN THE DIET ON PERFORMANCE AND SLAUGHTERING CHARACTERISTICS OF BROILERS |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |