CN107259186B - Laying hen feed for producing high-calcium high-zinc nutritional eggs and preparation method thereof - Google Patents
Laying hen feed for producing high-calcium high-zinc nutritional eggs and preparation method thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
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- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/153—Nucleic acids; Hydrolysis products or derivatives thereof
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/24—Compounds of alkaline earth metals, e.g. magnesium
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
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Abstract
The invention belongs to the field of feeds, and particularly relates to a laying hen feed for producing high-calcium high-zinc nutritional eggs and a preparation method thereof. The laying hen feed contains: base fermentation, calcium lactate, zinc gluconate, glycylglutamine and high nucleic acid yeast hydrolysate; the base stock fermentation product is a product obtained by inoculating bacillus cereus and bacillus megaterium to a layer feed base stock and performing ventilation fermentation; the inoculation amount of the bacillus cereus is 0.05-0.8 wt%, and the inoculation amount of the bacillus megaterium is 0.005-0.05 wt%; the content of calcium lactate is 8-15 wt%, the content of zinc gluconate is 0.1-0.5 wt%, the content of glycylglutamine is 0.01-0.5 wt%, and the content of high nucleic acid yeast hydrolysate is 0.1-0.5 wt%. The layer feed provided by the invention obviously improves the egg laying quality of the layers, and reduces the feed amount for feeding, thereby improving the production benefit of layer breeding.
Description
Technical Field
The invention relates to the field of feeds, and particularly relates to a laying hen feed for producing high-calcium high-zinc nutritional eggs and a preparation method thereof.
Background
The Feed (Feed) comprises more than ten kinds of Feed raw materials such as soybean, soybean meal, corn, fish meal, amino acid, miscellaneous meal, additives, whey powder, grease, meat and bone meal, grains, sweet sorghum and the like. The feed additive is a substance which is added in the feed of livestock and poultry in a small amount and is used for correcting nutritional deficiency caused by the lack of the feed ingredients or plays a special role in the growth of the livestock and poultry.
The eggs are rich in nutrient elements and can be used as carriers of various nutrient elements. The calcium and zinc elements are especially important for the health of human bodies. Although researchers add inorganic calcium and zinc sources with high dosage into feed as feed additives, the results show that calcium and zinc elements of edible parts in eggs are not increased, the calcium deposition amount of eggshells is increased, and the method causes the laying rate of the laying hens to be reduced, the laying period to be shortened and good effects are not obtained.
Therefore, the development of the laying hen feed which does not affect the laying rate and the laying period of the laying hen and can improve the content of calcium and zinc elements in eggs is urgently needed.
Disclosure of Invention
The invention aims to provide a laying hen feed for producing high-calcium high-zinc nutritional eggs and a preparation method thereof. The laying hen feed disclosed by the invention can be used for improving the laying rate of laying hens, prolonging the laying period, reducing the feed-egg ratio and the death rate of the laying hens, and obviously increasing the contents of trace elements such as calcium and zinc in eggs.
In order to achieve the purpose, the invention provides a layer feed for producing high-calcium high-zinc nutritional eggs, which contains: base fermentation, calcium lactate, zinc gluconate, glycylglutamine and high nucleic acid yeast hydrolysate;
the base stock fermentation product is a product obtained by inoculating bacillus cereus and bacillus megaterium to a layer feed base stock and performing ventilation fermentation;
based on the weight of the layer chicken feed base material, the inoculation amount of the bacillus cereus is 0.05-0.8 wt%, and the inoculation amount of the bacillus megaterium is 0.005-0.05 wt%;
based on the weight of the base material fermentation product, the content of calcium lactate is 8-15 wt%, the content of zinc gluconate is 0.1-0.5 wt%, the content of glycylglutamine is 0.01-0.5 wt%, and the content of the high nucleic acid yeast hydrolysate is 0.1-0.5 wt%.
In the invention, calcium lactate and zinc gluconate are healthy and safe organic chelated trace element feed additives, and are easier to be absorbed and utilized compared with inorganic feed additives.
The glutamine can provide a nitrogen source for proteins, amino acids, nucleic acids and the like, is a synthetic precursor of the proteins, the amino acids, the nucleic acids and the like, arginine generated by metabolism can indirectly regulate and control secretion of intestinal tracts, glycyl glutamine not only can have the functions, but also overcomes the problems of low solubility, instability and the like of the glutamine, and the glycyl glutamine has high water solubility, good stability and high absorption rate and can protect the small intestinal mucosa structure of the laying hens.
The high nucleic acid yeast hydrolysate can improve the villus height of animal small intestine, regulate the microbial balance of gastrointestinal tract and promote the absorption of nutrient substances in the intestinal tract, which plays an important role in the absorption of added organic chelated calcium and zinc by the laying hens. The concept of the nucleic acid-rich yeast hydrolysate in the present invention is well known in the art, and refers to a yeast hydrolysate with a high nucleotide content, preferably, the content of hydrolyzed nucleotides in the nucleic acid-rich yeast hydrolysate is 10-30%.
The bacillus cereus and the bacillus megaterium are high-temperature resistant, enter the intestinal tract to consume oxygen, are beneficial to the growth of native bacteria, can adjust the imbalance of intestinal flora, and can adjust the stress adverse reaction of the digestive tract caused by the high-calcium high-zinc feed additive by combining the bacillus cereus and the bacillus megaterium. And the feed has the characteristics of deodorization and improvement of the environment of the henhouse.
In the present invention, the layer feed base may be any of those known in the art as a feed base for feeding layers. Typically, they are commercially available or available in self-contained configurations.
According to a preferred embodiment of the present invention, the layer feed base comprises the following components, based on the total weight of the layer feed base:
3-8 wt%, preferably 4-6 wt%,
15-25 wt%, preferably 18-20 wt%,
5-10 wt%, preferably 6-9 wt%,
55-70 wt%, preferably 60-65 wt%,
3-8 wt%, preferably 4-6 wt% of pine needle powder.
The concept of the premix is well known to those skilled in the art, and refers to an additive premix feed, which is an intermediate compound feed product prepared by mixing one or more trace components (including various trace mineral elements, various vitamins, synthetic amino acids, certain drugs and other additives) with a diluent or a carrier according to the required proportion and uniformly mixing. The invention can use various premix compounds for laying hen feed in the field. Such premixes are generally commercially available. For example, in one embodiment of the present invention, the premix has a nutritional composition, based on the total weight of the premix: 170IU/kg of vitamin A, 340IU/kg of vitamin D, 340IU/kg of vitamin E, 200IU/kg of vitamin E, 320IU/kg of vitamin K, 130IU/kg of vitamin B, 250 IU/kg of vitamin B, 660 IU/kg of vitamin B, 200mg/kg of pantothenic acid, 400mg/kg of nicotinic acid, 30mg/kg of folic acid, 150mg/kg of copper, 1200mg/kg of iron, 800mg/kg of zinc, 1200mg/kg of manganese, 8 wt% of calcium, 3 wt% of total phosphorus, 3 wt% of sodium chloride, 1.2 wt% of lysine, 1.5 wt% of methionine and 10 wt% of water.
According to the invention, preferably, the inoculation amount of the bacillus cereus is 0.1-0.5 wt% and the inoculation amount of the bacillus megaterium is 0.01-0.02 wt% based on the weight of the laying hen feed base.
According to the present invention, preferably the calcium lactate is present in an amount of 10-12 wt.%, based on the weight of the layer chicken feed base fermented product.
According to the present invention, preferably, the zinc gluconate is contained in an amount of 0.3 to 0.4% by weight based on the weight of the layer chicken feed base fermented product.
According to the invention, the glycylglutamine content is preferably 0.05-0.2 wt% based on the weight of the layer chicken feed base fermented product.
According to the invention, the high nucleic acid yeast hydrolysate is preferably contained in an amount of 0.2 to 0.5 wt% based on the weight of the layer chicken feed base fermented product.
According to the invention, the inoculation temperature is preferably 70-85 ℃, and the higher temperature can meet the growth requirements of two strains on one hand and can effectively kill other mixed bacteria on the other hand. Before inoculation, a layer feed base stock can be crushed, added with water for modulation and sterilized, and the dosage of the layer feed base stock and the water is 1: 0.15-0.20.
The aeration fermentation can be carried out under the conventional environment, and the fermentation time can be determined according to the needs, and is preferably 18-36 hours.
The invention also provides a preparation method of the laying hen feed, which comprises the following steps: pulverizing feed base material of laying hen, adding water, sterilizing, inoculating Bacillus cereus and Bacillus megaterium, fermenting under ventilation, cooling fermented product, and adding calcium lactate, zinc gluconate, glycylglutamine and high nucleic acid yeast hydrolysate.
According to the invention, after fermentation is finished, other components are added after cooling, so that the added components are prevented from being degraded at high temperature. For example, the fermentation product may be cooled to below 40 ℃, typically to room temperature.
The sterilization method in the present invention is preferably microwave sterilization.
According to the invention, the organic chelate trace elements are combined with the gastrointestinal tract protective agent glycyl glutamine and the high-nucleic acid yeast hydrolysate, and then the feed is prepared by combining a probiotic fermentation technology, so that the laying rate of laying hens is improved after the laying hens are fed with the feed, the feed-egg ratio is reduced, the contents of trace elements such as calcium, zinc and the like in eggs are obviously increased, the death rate of the laying hens is reduced, and the purpose of efficiently producing high-calcium high-zinc nutritional eggs is realized.
Additional features and advantages of the invention will be set forth in the detailed description which follows.
Detailed Description
Preferred embodiments of the present invention will be described in more detail below. While the following describes preferred embodiments of the present invention, it should be understood that the present invention may be embodied in various forms and should not be limited by the embodiments set forth herein.
In the following examples, premix is purchased from modern agriculture and animal husbandry feed limited in Henan and the nutritional composition (based on the total weight of premix) is: 170IU/kg of vitamin A, 340IU/kg of vitamin D, 200IU/kg of vitamin E, 320IU/kg of vitamin K, 130IU/kg of vitamin B, 250 IU/kg of vitamin B, 660 IU/kg of vitamin B, 200mg/kg of pantothenic acid, 400mg/kg of nicotinic acid, 30mg/kg of folic acid, 150mg/kg of copper, 1200mg/kg of iron, 800mg/kg of zinc, 1200mg/kg of manganese, 8 wt% of calcium, 3 wt% of total phosphorus, 3 wt% of sodium chloride, 1.2 wt% of lysine, 1.5 wt% of methionine and 10 wt% of water.
In the following examples, the inoculation amounts of the two bacillus are based on the weight of the layer feed base material, the amounts of calcium lactate, zinc gluconate, glycylglutamine and high nucleic acid yeast hydrolysate are based on the weight content of the fermentation product of the base material, and the contents of the components in the layer feed base material are based on the total weight of the layer feed base material.
Example 1
This example prepared a layer feed.
The preparation process flow of the feed comprises the following steps: crushing a layer feed base material, adding water for modulation, uniformly mixing → performing microwave treatment for sterilization → inoculating 0.2% bacillus cereus and 160mg/kg bacillus megaterium at the high temperature of 80 ℃, performing ventilation fermentation for 24h → completing the fermentation, cooling to 37 ℃, adding 110g/kg calcium lactate, 3g/kg zinc gluconate, 0.1% glycylglutamine and 0.5% high nucleic acid yeast hydrolysate, and fully and uniformly mixing → bagging.
The laying hen feed base material comprises: 5% of premix; 19% of soybean meal; 8% of stone powder; corn flour of 63 percent; 5% of pine needle powder.
Example 2
This example prepared a layer feed.
The preparation process flow of the feed comprises the following steps: crushing a laying hen feed base material, adding water for modulation, uniformly mixing → performing microwave treatment for sterilization → inoculating 0.15% bacillus cereus and 200mg/kg bacillus megaterium at the high temperature of 75 ℃, performing ventilation fermentation for 24h → completing the fermentation, cooling to 25 ℃, adding 115g/kg calcium lactate, 3.5g/kg zinc gluconate, 0.08% glycylglutamine and 0.4% high nucleic acid yeast hydrolysate, fully mixing and bagging.
The laying hen feed base material comprises: 5% of premix; 19% of soybean meal; 8% of stone powder; corn flour of 63 percent; 5% of pine needle powder.
Example 3
This example prepared a layer feed.
The preparation process flow of the feed comprises the following steps: crushing a layer feed base material, adding water for modulation, uniformly mixing → performing microwave treatment for sterilization → inoculating 0.4% bacillus cereus and 120mg/kg bacillus megaterium at the high temperature of 85 ℃, performing ventilation fermentation for 24h → completing the fermentation, cooling to 37 ℃, adding 120g/kg calcium lactate, 4g/kg zinc gluconate, 0.2% glycylglutamine and 0.3% high nucleic acid yeast hydrolysate, and fully and uniformly mixing → bagging.
The laying hen feed base material comprises: 5% of premix; 19% of soybean meal; 8% of stone powder; corn flour of 63 percent; 5% of pine needle powder.
Test example
The influence of the feed on the production performance of the laying hens is tested.
Study subjects: 400 laying hens of about 130 days old in a cooperative culture base in a Wanghe power station hospital in a Meizhen Yun Yangyen area in Shi Wei city of Hubei province.
The test method comprises the following steps: randomly and averagely dividing the selected test chickens into four groups, wherein three groups are experimental groups, one group is a control group, the relative humidity is kept at 56-64% in the test period, and the illumination adopts a continuous illumination system, illumination is carried out for 23 hours, and darkness is carried out for 1 hour. The chicken coop is cleaned regularly to keep cleanness and sanitation by natural ventilation and free drinking and ingestion. Nipple type drinking water is adopted. The experimental groups were bred with the feeds of examples 1, 2, 3, respectively. The control group feed is a special formula feed for Yunyang pine needles in the breeding field.
And (3) test results: as shown in table 1 below.
TABLE 1
In table 1, all data are calculated in units of each group. The laying rate is equal to the number of eggs laid per day/the number of test chickens multiplied by 100 percent; the ratio of the feed to the egg is the amount of the feed used per total weight of the eggs; average egg weight is total egg weight/total egg amount; the average content of Ca/Zn is: (total Ca/Zn content of eggs)/total number of eggs; average feed intake ═ Σ (amount of feed fed per day-amount of feed at the end of feeding per day)/number of days fed.
As can be seen from the table 1, by using the layer feed, the laying rate, the feed-egg ratio, the average egg weight, the Ca content and the Zn content of the layer are obviously improved, the laying period is normal, the laying peak period is prolonged, the feed intake is reduced, and the death rate is also reduced. The laying hen feed provided by the invention obviously improves the egg laying quality of laying hens, and reduces the feed amount for feeding, thereby improving the production benefit of laying hen breeding.
Having described embodiments of the present invention, the foregoing description is intended to be exemplary, not exhaustive, and not limited to the embodiments disclosed. Many modifications and variations will be apparent to those of ordinary skill in the art without departing from the scope and spirit of the described embodiments.
Claims (8)
1. A layer feed for producing high-calcium high-zinc nutritional eggs is characterized by comprising the following components: base fermentation, calcium lactate, zinc gluconate, glycylglutamine and high nucleic acid yeast hydrolysate;
the base stock fermentation product is a product obtained by inoculating bacillus cereus and bacillus megaterium to a layer feed base stock and performing ventilation fermentation;
based on the weight of the layer chicken feed base material, the inoculation amount of the bacillus cereus is 0.05-0.8 wt%, and the inoculation amount of the bacillus megaterium is 0.005-0.05 wt%;
based on the weight of the base fermented product, the content of calcium lactate is 8-15 wt%, the content of zinc gluconate is 0.1-0.5 wt%, the content of glycylglutamine is 0.01-0.5 wt%, and the content of high nucleic acid yeast hydrolysate is 0.1-0.5 wt%;
the laying hen feed base material comprises the following components:
3-8% by weight of premix,
15-25% by weight of soybean meal,
5 to 10 weight percent of stone powder,
55-70 wt% of corn flour,
3-8 wt% of pine needle powder;
the preparation method of the laying hen feed comprises the following steps: pulverizing feed base material of laying hen, adding water, sterilizing, inoculating Bacillus cereus and Bacillus megaterium, fermenting under ventilation, cooling fermented product, and adding calcium lactate, zinc gluconate, glycylglutamine and high nucleic acid yeast hydrolysate.
2. The laying hen feed of claim 1, wherein the bacillus cereus is inoculated in an amount of 0.1-0.5 wt% and the bacillus megaterium is inoculated in an amount of 0.01-0.02 wt%, based on the weight of the laying hen feed base.
3. The laying hen feed of claim 1, wherein the calcium lactate is present in an amount of 10-12 wt% based on the weight of the laying hen feed base ferment.
4. The laying hen feed of claim 1, wherein the zinc gluconate is present in an amount of 0.3-0.4% by weight based on the weight of the base fermented product of the laying hen feed.
5. The laying hen feed of claim 1, wherein the glycylglutamine is present in an amount of 0.05-0.2 wt% based on the weight of the laying hen feed base fermented product.
6. The laying hen feed of claim 1, wherein the nucleic acid-rich yeast hydrolysate is present in an amount of 0.2 to 0.5 wt% based on the weight of the laying hen feed base fermented product.
7. The laying hen feed of claim 1, wherein the temperature of inoculation is 70-85 ℃ and the time of aeration fermentation is 18-36 hours.
8. The laying hen feed of claim 1, wherein the temperature of the fermentation product is reduced to below 40 ℃.
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| CN108740318A (en) * | 2018-08-29 | 2018-11-06 | 武汉轻工大学 | Produce the egg feedstuff and preparation method thereof of high ferro nutritional egg |
| CN109170314A (en) * | 2018-08-29 | 2019-01-11 | 武汉轻工大学 | Produce the egg feedstuff and preparation method thereof of homofolic acid nutritional egg |
| CN109221621A (en) * | 2018-11-15 | 2019-01-18 | 武汉轻工大学 | A kind of egg feedstuff and preparation method thereof producing high calcium high zinc nutritional egg |
| CN109480118A (en) * | 2018-11-22 | 2019-03-19 | 湖北菜鲜鲜食材供应链有限公司 | A kind of egg feedstuff and preparation method thereof producing high calcium high zinc nutritional egg |
| CN111109466B (en) * | 2019-12-18 | 2023-08-22 | 南京同凯兆业生物技术有限责任公司 | High-calcium high-potassium broussonetia papyrifera leaf feed additive and preparation method and application thereof |
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