CN109170314A - Produce the egg feedstuff and preparation method thereof of homofolic acid nutritional egg - Google Patents
Produce the egg feedstuff and preparation method thereof of homofolic acid nutritional egg Download PDFInfo
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- CN109170314A CN109170314A CN201810997715.7A CN201810997715A CN109170314A CN 109170314 A CN109170314 A CN 109170314A CN 201810997715 A CN201810997715 A CN 201810997715A CN 109170314 A CN109170314 A CN 109170314A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
- A23K20/147—Polymeric derivatives, e.g. peptides or proteins
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Abstract
The invention discloses a kind of egg feedstuffs and preparation method thereof for producing homofolic acid nutritional egg, contain base-material fermentation material, folic acid yeast autolyzate and glycylglutamine；On the basis of the weight of base-material fermentation material, folic acid yeast autolyzate is 5-7.5 weight %, and glycylglutamine is 0.01-0.5 weight %；Base-material fermentation material is the product that egg feedstuff base-material is inoculated with after bacillus cereus and bacillus megaterium and ventilating fermentation；Folic acid yeast autolyzate comprises the following steps: adding folic acid thereto in the growth logarithmic phase of brewer's yeast and growth stationary phase respectively, bacterium solution is collected in fermentation, prepares thallus；It will be mixed by the thallus of inactivation with neutral proteinase, self-dissolving；Cooling is centrifuged collected from solution, obtains sediment, is carried out frozen dried to the sediment, is obtained the folic acid yeast autolyzate.
Description
Technical field
The invention belongs to field of feed, more particularly, to a kind of egg feedstuff for producing homofolic acid nutritional egg
And preparation method thereof.
Background technique
Feed (Feed) includes soybean, dregs of beans, corn, fish meal, amino acid, miscellaneous dregs of rice, additive, whey powder, grease, meat bone
The feedstuff of more than ten kinds such as powder, cereal, sugar grass.Feed addictive is that one kind additive amount in livestock and poultry diet is seldom,
Deficiency disease or the substance of special role is played in the growth of livestock and poultry caused by correct the shortage due to diet ingredient.
The nutrient of egg is abundant, can be used as the carrier of various nutrients.Health of the folic acid element for human body
It is particularly important.Although there is researcher that feed is added using the folic acid of high dose as feed addictive, result is had been found that in egg
There is no increases for the folic acid element of edible portion, and laying rate of laying hen is caused to reduce, and ovipository cycle shortens, and there is no obtain
Good effect.
Therefore, it needs to develop a kind of neither influence laying rate of laying hen and ovipository cycle, moreover it is possible to improve folic acid element in egg
The egg feedstuff of content.
Summary of the invention
The purpose of the present invention is overcoming the deficiencies of existing technologies, a kind of egg feedstuff for producing homofolic acid nutritional egg is provided
And preparation method thereof, it using egg feedstuff of the invention, can be improved laying rate of laying hen, extend ovipository cycle, reduce feedstuff-egg ratio,
The content of folic acid element in egg is dramatically increased simultaneously.
To achieve the goals above, an aspect of of the present present invention provides a kind of egg feedstuff for producing homofolic acid nutritional egg,
The egg feedstuff contains: base-material fermentation material, folic acid yeast autolyzate and glycylglutamine；With the weight of the base-material fermentation material
On the basis of amount, the content of the folic acid yeast autolyzate is 5-7.5 weight %, and the content of the glycylglutamine is
0.01-0.5 weight %；
Wherein, the base-material fermentation material is that egg feedstuff base-material is inoculated with bacillus cereus and bacillus megaterium and divulges information
Product after fermentation；On the basis of the weight of the egg feedstuff base-material, the inoculum concentration of the bacillus cereus is 0.05-
0.8 weight %, the inoculum concentration of the bacillus megaterium are 0.005-0.05 weight %；
The folic acid yeast autolyzate comprises the following steps:
(1) folic acid is added thereto in the growth logarithmic phase of brewer's yeast and growth stationary phase respectively, then ferment, collect
Bacterium solution prepares thallus；(2) in the presence of protective gas and PH regulator, will be mixed by the thallus of inactivation with neutral proteinase,
Self-dissolving；(3) cooling is centrifuged collected from solution, obtains sediment, is then carried out frozen dried to the sediment, is obtained institute
State folic acid yeast autolyzate.
Another aspect of the present invention provides a kind of preparation method of above-mentioned egg feedstuff, method includes the following steps: will
Egg feedstuff base-material crushes, and water is added to modulate, and bacillus cereus and bacillus megaterium are inoculated with after sterilizing and carries out ventilating fermentation,
After tunning cooling, folic acid yeast autolyzate and glycylglutamine are added thereto.
Folic acid is added in yeast culture medium by the present invention, obtains a kind of chelating rich in folic acid by controlling zymotechnique
Small peptide, and frozen drying is carried out, folic acid yeast autolyzate is obtained, is added in egg feedstuff, one kind is made
The fermented feed for being suitble to laying hen edible.It is good using glycylglutamine stability, in animal body absorptivity it is high, can be effectively
Promote the conversion of microelement in animal body, and then laying hen enteron aisle is protected.Bacillus cereus, huge gemma simultaneously
Bacillus can adjust intestinal bacilli illness, be conducive to the growth of ancestral home bacterium, can eliminate the distress as caused by feed addictive
Reaction improves laying hen to the absorption rate of folic acid.Egg feedstuff of the invention can be improved laying rate of laying hen, extends laying hen and produces
The egg period.
Other features and advantages of the present invention will then part of the detailed description can be specified.
Specific embodiment
The preferred embodiment of the present invention is described in more detail below.Although the following describe preferred implementations of the invention
Mode, however, it is to be appreciated that may be realized in various forms the present invention without that should be limited by the embodiments set forth herein.Phase
Instead, these embodiments are provided so that the present invention is more thorough and complete, and can be by the scope of the present invention completely
It is communicated to those skilled in the art.
An aspect of of the present present invention provides a kind of egg feedstuff for producing homofolic acid nutritional egg, which contains: base
Expect fermentation material, folic acid yeast autolyzate and glycylglutamine；On the basis of the weight of the base-material fermentation material, the folic acid
The content of yeast autolyzate is 5-7.5 weight %, and the content of the glycylglutamine is 0.01-0.5 weight %；
Wherein, the base-material fermentation material is that egg feedstuff base-material is inoculated with bacillus cereus and bacillus megaterium and divulges information
Product after fermentation；On the basis of the weight of the egg feedstuff base-material, the inoculum concentration of the bacillus cereus is 0.05-
0.8 weight %, the inoculum concentration of the bacillus megaterium are 0.005-0.05 weight %；
The folic acid yeast autolyzate comprises the following steps:
(1) folic acid is added thereto in the growth logarithmic phase of brewer's yeast and growth stationary phase respectively, then ferment, collect
Bacterium solution prepares thallus；(2) in the presence of protective gas and PH regulator, will be mixed by the thallus of inactivation with neutral proteinase,
Self-dissolving；(3) cooling is centrifuged collected from solution, obtains sediment, is then carried out frozen dried to the sediment, is obtained institute
State folic acid yeast autolyzate.
Feed of the invention can improve the production efficiency and the quality of production of breeding layer chicken, increase folic acid in egg
Enriching quantity.By adding the bonding state that folic acid changes folic acid, and benefit in yeast different growing stages in yeast fermentation medium
The small peptide rich in folic acid is obtained with neutral protein enzymatic treatment yeast, is dried at low temperature after being collected by centrifugation and obtains folic acid
Yeast autolyzate；It is easier to be utilized by laying hen intestinal absorption compared to inorganic states feed addictive.
Glutamine can provide nitrogen source for synthesis such as protein, amino acid, nucleic acid, be protein, amino acid, nucleic acid
The synthesis precursor of equal substances, the arginine that metabolism generates can indirectly regulate and control the secretion of enteron aisle, glycylglutamine
Above-mentioned function can not only be possessed, and which overcome glutamine solubility is low, it is unstable the problems such as, glycylglutamine
Water solubility it is high, stability is good, and absorptivity is high, can protect laying hen small intestinal mucosa structure.
Bacillus cereus and bacillus megaterium high temperature resistant, can conducive to the growth of ancestral home bacterium into enteron aisle consumption oxygen
Adjust intestinal bacilli illness.
The present invention is by adding organic feed addictive, pipe intestinal protection agent and combining probiotics hot fermentation technology common
Laying hen production process is acted on, has achieved the purpose that efficient production homofolic acid egg.
In the present invention, folic acid is added in the growth logarithmic phase of brewer's yeast and growth stationary phase, it is more to facilitate folic acid
It is utilized by yeast.
In accordance with the present invention it is preferred that the step of preparation method of the folic acid yeast autolyzate (1) is respectively in beer ferment
Female growth logarithmic phase 10-27h and growth stationary phase 28-32h adds folic acid thereto in four times, then ferments, collects bacterium solution,
Prepare thallus；Wherein, the temperature of the fermentation is 30-40 DEG C, time 10-14h；Using the weight of the brewer's yeast as base
Standard, each additive amount of the folic acid are 2-4mg/kg.
In accordance with the present invention it is preferred that in the step of preparation method of the folic acid yeast autolyzate (2), the inactivation
Condition are as follows: inactivate 1-3h at humidity 90%-95%, 55-60 DEG C of temperature；The temperature of the self-dissolving is 50-60 DEG C, and the time is
18-22h, control PH are 6-7；On the basis of the weight of the thallus, the additive amount of the neutral proteinase is 0.3-0.7 weight
Measure %.
In accordance with the present invention it is preferred that being cooled to 2-6 DEG C in the step of preparation method of the folic acid yeast autolyzate (3)
Collected from solution.
In the present invention, the egg feedstuff base-material can be the object of the feed base-materials as raising laying hen various in this field
Matter.In general, commercially available or voluntarily configuration acquisition.
A kind of preferred embodiment according to the present invention, in the egg feedstuff base-material, with the total of the egg feedstuff base-material
Poidometer, the egg feedstuff base-material contain following components:
Premix 3-8 weight %, preferably 4-6 weight %,
Dregs of beans 15-25 weight %, preferably 18-20 weight %,
Mountain flour 5-10 weight %, preferably 6-9 weight %,
Corn flour 55-70 weight %, preferably 60-65 weight %,
Pine needle meal 3-8 weight %, preferably 4-6 weight %.
Wherein, the concept of the premix is known to the skilled person, and refers to additive premix, it be by
One or more microcomponents (including the addition such as various trace quantity mineral substance elements, various vitamins, synthesizing amino acid, some drugs
Agent) matched as required with diluent or carrier, after evenly mixing made of osculant compound feed products.This can be used in the present invention
The various premixes for egg feedstuff in field.The premix is usually commercially available.For example, one embodiment of the present invention
In, on the basis of the total weight of premix, the trophic component of the premix are as follows: vitamin A 170IU/kg, vitamin D
340IU/kg, vitamin E 200IU/kg, vitamin K 320IU/kg, vitamin B 130IU/kg, vitamin B2 50IU/kg,
Vitamin B6 60IU/kg, pantothenic acid 200mg/kg, niacin 400mg/kg, folic acid 30mg/kg, copper 150mg/kg, iron 1200mg/
Kg, zinc 800mg/kg, manganese 1200mg/kg, 8 weight % of calcium, 3 weight % of total phosphorus, 3 weight % of sodium chloride, 1.2 weight of lysine
Measure %, 1.5 weight % of methionine, 10 weight % of water.
In accordance with the present invention it is preferred that the bacillus cereus connects on the basis of the weight of the egg feedstuff base-material
Kind amount is 0.1-0.5 weight %, and the inoculum concentration of the bacillus megaterium is 0.01-0.02 weight %.
In accordance with the present invention it is preferred that on the basis of the weight of the egg feedstuff base-material fermentation material, the glycyl paddy ammonia
The content of amide is 0.05-0.2 weight %.
In accordance with the present invention it is preferred that the temperature of the inoculation is 70-85 DEG C, the time of the ventilating fermentation is that 18-36 is small
When.
According to the present invention, the temperature of the inoculation is preferably 70-85 DEG C, on the one hand higher temperature meets two kinds of bacterial strains
On the other hand growth demand can also effectively kill other miscellaneous bacterias.Before inoculation, egg feedstuff base-material can be crushed, water is added to modulate
And sterilize, the dosage of the egg feedstuff base-material and the water is 1:0.15-0.20.
Another aspect of the present invention provides a kind of preparation method of above-mentioned egg feedstuff, method includes the following steps: will
Egg feedstuff base-material crushes, and water is added to modulate, and bacillus cereus and bacillus megaterium are inoculated with after sterilizing and carries out ventilating fermentation,
After tunning cooling, folic acid yeast autolyzate and glycylglutamine are added thereto.
According to the present invention, it needs cooling to add other components after fermentation, is sent out at high temperature to avoid the component of addition
Raw degradation.For example, the tunning is cooled to 40 DEG C hereinafter, can usually be down to room temperature.
Sterilizing methods in the present invention are preferably microwave sterilization.
The present invention is further illustrated by the following examples:
In following embodiment, premix is purchased from Henan modern times agriculture and animal husbandry feed corporation,Ltd, and trophic component is (with premix
Total weight): vitamin A 170IU/kg, vitamin D 340IU/kg, vitamin E 200IU/kg, vitamin K 320IU/
Kg, vitamin B 130IU/kg, vitamin B2 50IU/kg, vitamin B6 60IU/kg, pantothenic acid 200mg/kg, niacin 400mg/
Kg, folic acid 30mg/kg, copper 150mg/kg, iron 1200mg/kg, zinc 800mg/kg, manganese 1200mg/kg, 8 weight % of calcium, total phosphorus 3
Weight %, 3 weight % of sodium chloride, 1.2 weight % of lysine, 1.5 weight % of methionine, 10 weight % of water.
In following embodiment, the inoculum concentration of two kinds of bacillus is in terms of the weight of egg feedstuff base-material, and folic acid yeast is certainly
The amount of molten object and glycylglutamine is the weight content in terms of base-material fermentation material, the content of each component in egg feedstuff base-material
For with the weight content of the total weight of egg feedstuff base-material.
Embodiment 1
The present embodiment prepares a kind of egg feedstuff.
Feed preparation process flow are as follows: egg feedstuff base-material is crushed, water modulation, mixing → microwave treatment sterilization are added
80 DEG C of → high temperature inoculations 0.2% bacillus cereus, 160mg/kg bacillus megaterium, ventilating fermentation for 24 hours → fermentation finish, drop
Temperature adds 5% folic acid yeast autolyzate, 0.1% glycylglutamine is mixed well → packed to 37 DEG C.
The egg feedstuff base-material includes: premix 5%；Dregs of beans 19%；Mountain flour 8%；Corn flour 63%；Pine needle meal 5%.
The folic acid yeast autolyzate is made by the following method: logarithmic phase 10h, 20h is grown in brewer's yeast respectively, it is raw
Long stationary phase 28h, 32h in four times in its culture medium in each every kilogram of brewer's yeast add 2mg folic acid, 35 DEG C of temperature,
Shake flask fermentation 12h, collects bacterium solution, centrifugal drying obtain thallus → at humidity 90%-95%, 55 DEG C -60 DEG C of temperature inactivate 2h →
Into enzymatic vessel (enzymatic vessel uses preceding preparatory exclusion oxygen, is filled with certain volume nitrogen), 0.5% neutral proteinase is added,
0.3%NaCl is adjusted to pH6.0, and self-dissolving 20h → low temperature is down to 4 DEG C, 3000r/min under the conditions of 55 DEG C of temperature, is centrifuged 20min, right
Sediment carries out -40 DEG C of frozen drying → vacuum bagging.
Embodiment 2
The present embodiment prepares a kind of egg feedstuff.
Feed preparation process flow are as follows: egg feedstuff base-material is crushed, water modulation, mixing → microwave treatment sterilization are added
80 DEG C of → high temperature inoculations 0.2% bacillus cereus, 160mg/kg bacillus megaterium, ventilating fermentation for 24 hours → fermentation finish, drop
Temperature adds 6% folic acid yeast autolyzate, 0.1% glycylglutamine is mixed well → packed to 37 DEG C.
The egg feedstuff base-material includes: premix 5%；Dregs of beans 19%；Mountain flour 8%；Corn flour 63%；Pine needle meal 5%.
The folic acid yeast autolyzate is made by the following method: logarithmic phase 10h, 20h is grown in brewer's yeast respectively, it is raw
Long stationary phase 28h, 32h in four times in its culture medium in each every kilogram of brewer's yeast add 3mg folic acid, 35 DEG C of temperature,
Shake flask fermentation 12h, collects bacterium solution, centrifugal drying obtain thallus → at humidity 90%-95%, 55 DEG C -60 DEG C of temperature inactivate 2h →
Into enzymatic vessel (enzymatic vessel uses preceding preparatory exclusion oxygen, is filled with certain volume nitrogen), 0.5% neutral proteinase is added,
0.3%NaCl is adjusted to pH6.0, and self-dissolving 20h → low temperature is down to 4 DEG C, 3000r/min under the conditions of 55 DEG C of temperature, is centrifuged 20min, right
Sediment carries out -40 DEG C of frozen drying → vacuum bagging.
Embodiment 3
The present embodiment prepares a kind of egg feedstuff.
Feed preparation process flow are as follows: egg feedstuff base-material is crushed, water modulation, mixing → microwave treatment sterilization are added
80 DEG C of → high temperature inoculations 0.2% bacillus cereus, 160mg/kg bacillus megaterium, ventilating fermentation for 24 hours → fermentation finish, drop
Temperature adds 7.5% folic acid yeast autolyzate, 0.1% glycylglutamine is mixed well → packed to 37 DEG C.
The egg feedstuff base-material includes: premix 5%；Dregs of beans 19%；Mountain flour 8%；Corn flour 63%；Pine needle meal 5%.
The folic acid yeast autolyzate is made by the following method: logarithmic phase 10h, 20h is grown in brewer's yeast respectively, it is raw
Long stationary phase 28h, 32h in four times in its culture medium in each every kilogram of brewer's yeast add 4mg folic acid, 35 DEG C of temperature,
Shake flask fermentation 12h, collects bacterium solution, centrifugal drying obtain thallus → at humidity 90%-95%, 55 DEG C -60 DEG C of temperature inactivate 2h →
Into enzymatic vessel (enzymatic vessel uses preceding preparatory exclusion oxygen, is filled with certain volume nitrogen), 0.5% neutral proteinase is added,
0.3%NaCl is adjusted to pH6.0, and self-dissolving 20h → low temperature is down to 4 DEG C, 3000r/min under the conditions of 55 DEG C of temperature, is centrifuged 20min, right
Sediment carries out -40 DEG C of frozen drying → vacuum bagging.
Comparative example 1
The difference of this comparative example and embodiment 1 are as follows: the folic acid yeast autolyzate is made by the following method: in beer ferment
The folic acid of 8mg is added in female culture medium in every kilogram of brewer's yeast, 35 DEG C of temperature, shake flask fermentation 12h, collects bacterium solution, centrifugation
Dry thallus → 2h is inactivated at humidity 90%-95%, 55 DEG C -60 DEG C of temperature → enters enzymatic vessel, and (enzymatic vessel uses preceding pre-
Oxygen is first excluded, certain volume nitrogen is filled with), 0.5% neutral proteinase is added, 0.3%NaCl is adjusted to pH 6.0, and 55 DEG C of temperature
Under the conditions of self-dissolving 20h → low temperature be down to 4 DEG C, 3000r/min, be centrifuged 20min, -40 DEG C of frozen dryings carried out to sediment
→ vacuum bagging.
Other egg feedstuff preparation process flows and parameter and egg feedstuff each component and dosage with 1 phase of embodiment
Together.
Comparative example 2
This comparative example prepares a kind of egg feedstuff.
Feed preparation process flow are as follows: egg feedstuff base-material is crushed, water modulation, mixing → microwave treatment sterilization are added
80 DEG C of → high temperature inoculations 0.2% bacillus cereus, 160mg/kg bacillus megaterium, ventilating fermentation for 24 hours → fermentation finish, drop
Temperature is to 37 DEG C, addition 2mg/kg folic acid, 0.1% glycylglutamine, 0.5% high-nucleic acid yeast hydrolysate, mix well →
Pack；Wherein, the inoculum concentration of two kinds of bacillus is in terms of the weight of egg feedstuff base-material, folic acid, glycylglutamine and
The amount of high-nucleic acid yeast hydrolysate is in terms of the weight of base-material fermentation material.
With the total weight of egg feedstuff base-material, the egg feedstuff base-material includes: premix 5%；Dregs of beans 19%；Mountain flour
8%；Corn flour 63%；Pine needle meal 5%.
Test case
Test influence of the feed to performance in layers.
Research object: 400 130 of the area Yun Yang of Shiyan City of the Hubei Province town the Mei Pu power station Wang He Yuan Nei cooperative society cultivation base
The laying hen of age in days or so.
Test method: being divided into six groups with chicken stochastic averagina for the test of selection, wherein three groups are experimental group, three groups are control
Group, experimental period relative humidity are maintained at 56%-64%, and continuous illumination system, 23h illumination, 1h dark are taken in illumination.Naturally logical
Wind, free water and feeding, periodically sweep henhouse, keep hygienically clean.It is drunk water using nipple-shape.Implementation is respectively adopted in experimental group
The feed of example 1,2,3 is cultivated.1 feed of control group is feed prepared by comparative example 1, and 2 feed of control group is the preparation of comparative example 2
Feed, 3 feed of control group be farm's Yun county sun pine needle egg proprietary formula material.
Test result: as shown in table 1 below.
Table 1
| Feed intake | Average egg weight | Laying rate | Feedstuff-egg ratio | Folic acid average content | |
| Embodiment 1 | 114g | 60g | 96% | 1.6 | 1.3mg/kg |
| Embodiment 2 | 112g | 62g | 97% | 1.7 | 1.5mg/kg |
| Embodiment 3 | 110g | 63g | 97% | 1.7 | 1.9mg/kg |
| Control group 1 | 115g | 53g | 93% | 2.0 | 0.75mg/kg |
| Control group 2 | 116g | 52g | 92% | 2.1 | 0.6mg/kg |
| Control group 3 | 116g | 51g | 90% | 2.3 | 0.45mg/kg |
In table 1, all data are calculated as unit of every group.Laying rate=day egg production/test chicken quantity ×
100%；Doses/egg the total weight for feedstuff-egg ratio=use；Average egg weight=egg total weight/egg total quantity；Folic acid is average
Content are as follows: the total quantity of (total folate content of egg)/egg；Average feed intake=∑ (feeds forage volume-to feed daily daily
At the end of scale of feeding)/feeding number of days.
As it can be seen from table 1 the laying rate of laying hen, average egg weight, folate content have using egg feedstuff of the invention
It significantly improves, feedstuff-egg ratio and feed intake decrease.Illustrate that egg feedstuff of the invention significantly improves Egg Production of Laying Hens quality, and
The forage volume fed is reduced, to improve the production interests of breeding layer chicken.
Various embodiments of the present invention are described above, above description is exemplary, and non-exclusive, and
It is not limited to disclosed each embodiment.Without departing from the scope and spirit of illustrated each embodiment, for this skill
Many modifications and changes are obvious for the those of ordinary skill in art field.
Claims (10)
1. a kind of egg feedstuff for producing homofolic acid nutritional egg, which is characterized in that the egg feedstuff contains: base-material fermentation material,
Folic acid yeast autolyzate and glycylglutamine；On the basis of the weight of the base-material fermentation material, the folic acid yeast autolysis
The content of object is 5-7.5 weight %, and the content of the glycylglutamine is 0.01-0.5 weight %；
Wherein, the base-material fermentation material is that egg feedstuff base-material is inoculated with bacillus cereus and bacillus megaterium and ventilating fermentation
Product afterwards；On the basis of the weight of the egg feedstuff base-material, the inoculum concentration of the bacillus cereus is 0.05-0.8 weight
% is measured, the inoculum concentration of the bacillus megaterium is 0.005-0.05 weight %；
The folic acid yeast autolyzate comprises the following steps:
(1) folic acid is added thereto in the growth logarithmic phase of brewer's yeast and growth stationary phase respectively, then ferment, collect bacterium
Liquid prepares thallus；(2) it in the presence of protective gas and PH regulator, will be mixed by the thallus of inactivation with neutral proteinase, certainly
It is molten；(3) cooling is centrifuged collected from solution, obtains sediment, then carries out frozen dried to the sediment, is obtained described
Folic acid yeast autolyzate.
2. egg feedstuff according to claim 1, wherein the step of the preparation method of the folic acid yeast autolyzate (1)
To add folic acid thereto in four times in the growth logarithmic phase 10-27h of brewer's yeast and growth stationary phase 28-32h respectively, then
Bacterium solution is collected in fermentation, prepares thallus；
Wherein, the temperature of the fermentation is 30-40 DEG C, time 10-14h；It is described on the basis of the weight of the brewer's yeast
Each additive amount of folic acid is 2-4mg/kg.
3. egg feedstuff according to claim 1, wherein the step of the preparation method of the folic acid yeast autolyzate (2)
In, the condition of the inactivation are as follows: inactivate 1-3h at humidity 90%-95%, 55-60 DEG C of temperature；The temperature of the self-dissolving is 50-
60 DEG C, time 18-22h, control PH are 6-7；On the basis of the weight of the thallus, the additive amount of the neutral proteinase is
0.3-0.7 weight %.
4. egg feedstuff according to claim 1, wherein the step of the preparation method of the folic acid yeast autolyzate (3)
In, 2-6 DEG C is cooled to collected from solution.
5. egg feedstuff according to claim 1, wherein with the total weight of the egg feedstuff base-material, the laying hen
Feed base-material contains following components:
6. egg feedstuff according to claim 1, wherein on the basis of the weight of the egg feedstuff base-material, the wax
The inoculum concentration of sample bacillus is 0.1-0.5 weight %, and the inoculum concentration of the bacillus megaterium is 0.01-0.02 weight %.
7. egg feedstuff according to claim 1, wherein on the basis of the weight of the egg feedstuff base-material fermentation material,
The content of the glycylglutamine is 0.05-0.2 weight %.
8. egg feedstuff according to claim 1, wherein the temperature of the inoculation is 70-85 DEG C, the ventilating fermentation
Time is 18-36 hours.
9. the preparation method of egg feedstuff described in any one of claim 1-8, which is characterized in that this method includes following
Step: egg feedstuff base-material being crushed, water is added to modulate, and bacillus cereus and bacillus megaterium are inoculated with after sterilizing and is led to
Folic acid yeast autolyzate and glycylglutamine is added after tunning cooling in wind fermentation thereto.
10. preparation method according to claim 9, wherein the tunning is cooled to 40 DEG C or less.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810997715.7A CN109170314A (en) | 2018-08-29 | 2018-08-29 | Produce the egg feedstuff and preparation method thereof of homofolic acid nutritional egg |
Applications Claiming Priority (1)
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| CN112369532A (en) * | 2020-12-11 | 2021-02-19 | 辽宁众友饲料有限公司 | Laying hen feed and preparation method and application thereof |
| CN114601070A (en) * | 2022-03-21 | 2022-06-10 | 山西农业大学 | Laying hen feed additive and preparation method and application thereof |
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| CN114601070B (en) * | 2022-03-21 | 2023-08-25 | 山西农业大学 | Laying hen feed additive and preparation method and application thereof |
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Application publication date: 20190111 |