CN107048048A - A kind of gene editing tests mouse feed - Google Patents
A kind of gene editing tests mouse feed Download PDFInfo
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- CN107048048A CN107048048A CN201710390093.7A CN201710390093A CN107048048A CN 107048048 A CN107048048 A CN 107048048A CN 201710390093 A CN201710390093 A CN 201710390093A CN 107048048 A CN107048048 A CN 107048048A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
Mouse feed is tested the invention discloses a kind of gene editing, includes each component of following parts by weight：30 35 parts of corn, 10 15 parts of wheat, 15 20 parts of dregs of beans, crack rice 5 12 parts, 3 10 parts of fish meal, oil 15 parts, 29 parts of yeast hydrolyate, 0.8 1.2 parts of stone flour, 12 parts of calcium monohydrogen phosphate, 0.5 1 parts of composite mineral matter, 0.1 0.5 parts of B B-complex, 0.1 0.2 parts of methionine, 0.1 0.2 parts of lysine.Nutrition of the present invention is balanced, feed palatability is good, by increasing capacitance it is possible to increase gene editing experimental mouse feed intake, promotes growth reproduction effect substantially, significantly improves the survival rate of gene editing experimental mouse, its nutritive index meets《Experimental animal full nutrition feed》The requirement of nutritive index in standard GB/T 14924.3 2010, sanitary index meets《Experimental animal full nutrition feed》The requirement of sanitary index in standard GB/T 14924.1 2010, gene editing experimental mouse feed applications of the present invention have good prospects.
Description
Technical field
The invention belongs to experimental animal alimentary field, mouse feed is tested more particularly, to a kind of gene editing.
Background technology
Gene editing technology refers to allow the mankind to enter target gene edlin, realizes the knockout to specific DNA fragments, adds
Enter.Mainly there are three below executive meanses：（1）Gene knockout：If it is desired to the function of some gene is lost, can be at this
Double-strand DNA cleavage is produced on gene（DSB）, DNA insertion is often produced during non-homologous end joining reparation or is deleted
Remove, cause frameshift mutation, so as to realize gene knockout；（2）Specific mutagenesis is introduced：If it is desired to some special mutation is incorporated into
, it is necessary to be realized by homologous recombination on genome, at this moment provide one and contain specific mutagenesis homologous templates.Normal condition is similarly hereinafter
Source recombination efficiency is very low, and recombination efficiency can greatly be improved by producing DSB in this site, so as to realize drawing for specific mutagenesis
Enter；（3）Pinpoint transgenosis：As the principle that specific mutagenesis is introduced, a transgenosis, this turn are added in the middle of homologous templates
Gene can be copied into genome in DSB repair processes, so as to realize fixed point transgenosis.By pinpointing transgene method
Gene can be inserted into the genome AAVS1 sites of people, this site is an open site, support that transgenosis is steady in a long-term
Expression, destroying this site does not have harmful effect to cell, therefore is widely used.
At present, with the maturation of gene editing technology, by the experiment rat animal model of gene editing technical finesse in people
The status of the research field of class disease is increasingly important.Particularly experimental mouse and human genome very high homology, for human diseases
Feature is consistent with effect of drugs reaction height, therefore experimental mouse has been most important experimental animal in human diseases research.
Due to being markedly less than normal experiment mouse by the experimental mouse function of immune system after gene editing, therefore it has life
The features such as length is slow, reproduction ability is low, the experimental mouse fed using conventional experiment mouse feed after gene editing can cause battalion
Support bad, fecundity to degenerate and cause experimental mouse dead, it is therefore desirable to research and develop special feed for gene editing experimental mouse.
The content of the invention
For above-mentioned deficiency of the prior art, inventor passes through scientific design, Germicidal efficacy, have developed a kind of gene and compiles
Experiment mouse feed is collected, for the growth and breeding by the model experiment mouse after gene editing technical finesse.
The above-mentioned purpose of the present invention is achieved by the following technical programs.
A kind of gene editing tests mouse feed, includes each component of following parts by weight：30-35 parts of corn, wheat 10-15
Part, 15-20 parts of dregs of beans, crack rice 5-12 parts, 3-10 parts of fish meal, 1-5 parts of oil, 2-9 parts of yeast hydrolyate, 0.8-1.2 parts of stone flour, phosphorus
Sour 1-2 parts of hydrogen calcium, 0.5-1 parts of composite mineral matter, 0.1-0.5 parts of B B-complex, 0.1-0.2 parts of methionine, lysine 0.1-
0.2 part.
Due to the gene editing experimental mouse physiological property of itself, if easily caused after being fed with conventional experiment mouse feed
Experimental mouse is slow-growing, it is even dead to breed degraded performance, by the present invention in that with corn, wheat, dregs of beans, crack rice, fish meal,
Oil, yeast hydrolyate, stone flour, calcium bicarbonate, composite mineral matter, B B-complex, methionine, lysine are that experiment is made in raw material
Mouse feed, with palatability is good, nutrition is balanced, promote growth reproduction effect obvious, by increasing capacitance it is possible to increase the work(of feed intake and survival rate
Effect.Specifically, the dregs of beans has the peat-reek of dregs of beans as a kind of byproduct after soybean extracting bean oil, is experimental mouse
One of staple food of feed.The yeast hydrolyate is the hydrolysate of yeast cells, is obtained by self-dissolving or by additional enzyme hydrolysis
Arrive, rich in amino acid, small peptide, glutathione and nucleic acid material.On the one hand dregs of beans, yeast hydrolyate have abundant in this formula
Nutrition, can with the methionine, lysine cooperate, and with the B B-complex coordinate, be gene editing experiment
Mouse provides required amino acid, enzyme, polypeptide, lactic acid, vitamin, somatomedin etc.；On the other hand, in yeast hydrolyate
Polynucleotide material, and dregs of beans can produce a large amount of small peptides with unique physiological active functionses through probiotics fermention hydrolytic process and mix
The propagation of beneficial bacterium in compound, it is easy to digest and assimilate, effective stimulus enteron aisle, adjusts the structure of internal Tiny ecosystem flora, increases whole
Individual alimentary canal is to the decomposition of feed nutrition material, synthesis, absorption and utilizes, and has enhancing immunity of organism to gene editing experimental mouse
Power, promotion cytothesis and reparation, promotion intestinal growth, effect that is anti-oxidant and maintaining normal intestinal flora, it is to avoid enteron aisle disease
The generation of disease, improves utilization rate of the experimental mouse to feed protein and energy；Furthermore inosinicacid and guanosine in yeast hydrolyate
Acid, can be as fresh flavor agent be increased with reference to distinctive fermenting aroma, and palatability is good, increases the feed intake of animal.In addition, the stone
Powder, calcium monohydrogen phosphate and meet mineral matter cooperate provide experimental mouse needed for mineral nutrient element, feed digestion can be promoted,
Make experimental mouse increased weight.
To sum up, inventor goes out this feed formula by a large amount of scientific designs, Germicidal efficacy research preparation, and each component optimization is matched somebody with somebody
Than efficiently solving that existing gene editing experimental mouse is slow-growing, breed the situation that performance is low and the death rate is low；Compiled for gene
The physiological characteristic of volume experimental mouse, makes that palatability is good, nutrition feed in a balanced way, meets the need that the production of gene editing experimental mouse is bred
Ask, feed increase yeast hydrolyate promotes experimental mouse abilities of digestive and absorption, lifts experimental mouse feed intake.All nutritive indexs of feed
It is satisfied by national standard requirement, microorganism and the equal control word national standard allowed band of sanitary index.Nutrition of the present invention is balanced, promote
Growth reproduction effect is obvious, steady quality is reliable.
Preferably, it is made up of each component of following parts by weight：30-33 parts of corn, 13-15 parts of wheat, dregs of beans 19.5-20
Part, crack rice 10-12 parts, 8-8.5 parts of fish meal, 2.5-3 parts of oil, 7.4-8.5 parts of yeast hydrolyate, 1.1-1.2 parts of stone flour, phosphoric acid hydrogen
1.0 parts of calcium, 0.6 part of composite mineral matter, 0.4 part of B B-complex, 0.2 part of methionine, 0.2 part of lysine.
Preferably, it is made up of each component of following parts by weight：30 parts of corn, 15 parts of wheat, 20 parts of dregs of beans, crack rice 12
Part, 8 parts of fish meal, 3 parts of oil, 8.4 parts of yeast hydrolyate, 1.2 parts of stone flour, 1 part of calcium monohydrogen phosphate, 0.6 part of composite mineral matter, compound dimension
Raw 0.4 part of element, 0.2 part of methionine, 0.2 part of lysine.
Preferably, the preparation method of the yeast hydrolyate is as follows：Using fresh saccharomyces cerevisiae as raw material, through self-dissolving, enzymolysis,
Concentration, dry refine form.Yeast hydrolyate made from this method can significantly improve the feed intake of experimental mouse, with other yeast
Hydrolysate is compared, and mellow in taste is moderate, without bitterness sense.
Preferably, the mass percent in the composite mineral matter shared by each element is：Copper 6%, iron 6%, manganese 1.3%, zinc
4.2%th, selenium 0.3%, magnesium 1.5%, iodine 0.13%, cobalt 0.02%, surplus are carrier zeolite powder.
Preferably, the B B-complex is vitamin A, vitamin C, vitamin D, vitamin E, vitamin K, pantothenic acid
Two or more mixtures in calcium, nicotinic acid, biotin or folic acid.
Preferably, the oil is vegetable oil；The dregs of beans is soyabean expeller, and the content of its thick protein reaches 40 ~ 50%；Institute
Corn is stated for northeast corn.
The present invention also provides the preparation method that the gene editing tests mouse feed, comprises the following steps：S1. raw material is matched somebody with somebody
System：After corn, wheat, soyabean expeller and crushing of cracking rice, it is sufficiently mixed with surplus stock；S2. modulation granulation：Will mixing
Good raw material input granulator, pellet is made under 80-120 DEG C of steam；S3. feed is screened：By what is made
Pellet is screened, and retains particle diameter 11-13mm, and length is 30-40mm pellet；S4. nutritional surveillance and packaging：
Nutritive index, sanitary index, microbial indicators detected are carried out to the feed screened, wrapped for standard compliant feed
Dress, storage.
It is highly preferred that the particle diameter 12mm of the pellet.
Compared with prior art, beneficial effect of the present invention is：Nutrition of the present invention is balanced, promote growth reproduction effect bright
It is aobvious；Palatability is good, by increasing capacitance it is possible to increase gene editing experimental mouse feed intake；The present invention can effectively reduce the dead of gene editing experimental mouse
The rate of washing in a pan, hence it is evident that improve the speed of growth, promotes intestinal health, reduces enterogastric diseases incidence；Rich in feature small peptide and a variety of
Ferment, digestibility is high, can effectively improve efficiency of feed utilization, rich in nucleotides, active peptide and immune polysaccharide isoreactivity composition,
The non-specific immunity of animal is remarkably improved, strengthens premunition；Nutritive index meets《Experimental animal full nutrition feed》
The requirement of nutritive index in standard GB/T 14924.3-2010, sanitary index meets《Experimental animal full nutrition feed》Country
The requirement of sanitary index in standard GB14924.1-2010, has preferable application prospect in terms of experimental mouse feed is promoted.
Embodiment
In order to make the purpose , technical scheme and advantage of the present invention be clearer, with reference to embodiments, to present invention progress
It is further described.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to limit
The present invention.Unless stated otherwise, the present invention is used reagent, method and apparatus for the art conventional reagent, method and are set
It is standby.
It has been investigated that, feed effect that feed of the present invention is made compared to conventional method according to following preparation method compared with
It is excellent, specifically include following steps：S1. raw material is prepared：After corn, wheat, dregs of beans and crushing of cracking rice, carried out with surplus stock
It is sufficiently mixed；S2. modulation granulation：The raw material mixed is put into granulator, particle is made under 80-120 DEG C of steam
Feed；S3. feed is screened：The pellet made is screened, retains particle diameter 11-13mm, length is 30-40mm's
Pellet；S4. nutritional surveillance and packaging：Feed sterilization to having screened, and carry out nutritive index, sanitary index, microorganism
Indexs measure, is packed for standard compliant feed, is stored.
If without specified otherwise, the raw material uses commercially available material.
The inventive method is further described by taking specific implementation condition as an example below.
Embodiment 1
A kind of gene editing tests mouse feed, is made up of each component of following parts by weight：30 parts of corn, 15 parts of wheat, dregs of beans
20 parts, crack rice 12 parts, 8 parts of fish meal, oil 3 parts, 8.4 parts of yeast hydrolyate, 1.2 parts of stone flour, 1 part of calcium monohydrogen phosphate, composite mineral matter
0.6 part, 0.4 part of B B-complex, 0.2 part of methionine, 0.2 part of lysine.
The preparation method of above-mentioned feed comprises the following steps：S1. raw material is prepared：By corn, wheat, dregs of beans and powder of cracking rice
After broken, it is sufficiently mixed with surplus stock；S2. modulation granulation：The raw material mixed is put into granulator, in 100-
Pellet is made under 120 DEG C of steam；S3. feed is screened：The pellet made is screened, retains particle diameter
12mm, length is 30-40mm pellet；S4. nutritional surveillance and packaging：Nutritive index is carried out to the feed screened, defended
Raw index, microbial indicators detected, are packed for standard compliant feed, are stored.
Embodiment 2
A kind of gene editing tests mouse feed, is made up of each component of following parts by weight：32.4 parts of corn, 13 parts of wheat, beans
19.6 parts of the dregs of rice, crack rice 12 parts, it is 8.5 parts of fish meal, 2.5 parts of oil, 8.5 parts of yeast hydrolyate, 1.1 parts of stone flour, 1 part of calcium monohydrogen phosphate, multiple
Close 0.6 part of mineral matter, 0.4 part of B B-complex, 0.2 part of methionine, 0.2 part of lysine.
The preparation method of above-mentioned feed is same as Example 1.
Embodiment 3
A kind of gene editing tests mouse feed, is made up of each component of following parts by weight：33 parts of corn, 15 parts of wheat, dregs of beans
20 parts, crack rice 10 parts, 8 parts of fish meal, oil 3 parts, 7.4 parts of yeast hydrolyate, 1.2 parts of stone flour, 1 part of calcium monohydrogen phosphate, composite mineral matter
0.6 part, 0.4 part of B B-complex, 0.2 part of methionine, 0.2 part of lysine.
The preparation method of above-mentioned feed is same as Example 1.
Embodiment 4
A kind of gene editing tests mouse feed, is made up of each component of following parts by weight：35 parts of corn, 12.5 parts of wheat, beans
18.8 parts of the dregs of rice, crack rice 5 parts, 10 parts of fish meal, oil 5 parts, 9 parts of yeast hydrolyate, 0.8 part of stone flour, 2 parts of calcium monohydrogen phosphate, complex mineral
1 part of matter, 0.5 part of B B-complex, 0.2 part of methionine, 0.2 part of lysine.
The preparation method of above-mentioned feed is same as Example 1.
Embodiment 5
A kind of gene editing tests mouse feed, is made up of each component of following parts by weight：33.9 parts of corn, 10 parts of wheat, beans
15 parts of the dregs of rice, crack rice 12 parts, 10 parts of fish meal, oil 5 parts, 9 parts of yeast hydrolyate, 1.2 parts of stone flour, 2 parts of calcium monohydrogen phosphate, composite mineral matter
1 part, 0.5 part of B B-complex, 0.2 part of methionine, 0.2 part of lysine.
The preparation method of above-mentioned feed is same as Example 1.
Embodiment 6
A kind of gene editing tests mouse feed, is made up of each component of following parts by weight：35 parts of corn, 15 parts of wheat, dregs of beans
20 parts, crack rice 5 parts, 10 parts of fish meal, oil 5 parts, 6 parts of yeast hydrolyate, 1.2 parts of stone flour, 2 parts of calcium monohydrogen phosphate, composite mineral matter 0.5
Part, 0.1 part of B B-complex, 0.1 part of methionine, 0.1 part of lysine.
The preparation method of above-mentioned feed is same as Example 1.
Embodiment 7
A kind of gene editing tests mouse feed, is made up of each component of following parts by weight：35 parts of corn, 15 parts of wheat, dregs of beans
20 parts, crack rice 7.9 parts, 3 parts of fish meal, oil 5 parts, 9 parts of yeast hydrolyate, 1.2 parts of stone flour, 2 parts of calcium monohydrogen phosphate, composite mineral matter 1
Part, 0.5 part of B B-complex, 0.2 part of methionine, 0.2 part of lysine.
The preparation method of above-mentioned feed is substantially same as Example 1, and difference is, S2 institutes in step in preparation method
The refining temperature for stating steam is 80-100 DEG C.
Comparative example 1
This comparative example and a kind of gene editing of embodiment 1 experiment mouse feed are essentially identical, specifically by each group of following parts by weight
It is grouped into：32.4 parts of corn, 13 parts of wheat, 14 parts of dregs of beans, crack rice 12 parts, 8.5 parts of fish meal, oily 2.5 parts, yeast hydrolyate 12
Part, 1.1 parts of stone flour, 1 part of calcium monohydrogen phosphate, 0.6 part of composite mineral matter, 2.5 parts of B B-complex, 0.2 part of methionine, lysine
0.2 part.
Comparative example 2
This comparative example and a kind of gene editing of embodiment 1 experiment mouse feed are essentially identical, and difference is the yeast water
Solution thing replaces with fodder yeast, and its constituent content is constant.
Comparative example 3
A kind of gene editing experiment mouse feed of this comparative example is essentially identical, is specifically made up of each component of following parts by weight：It is beautiful
Rice 35 parts, 13 parts of wheat, 20 parts of dregs of beans, crack rice 8 parts, 5.6 parts of fish meal, oil 2.5 parts, 5 parts of yeast hydrolyate, 1.1 parts of stone flour, phosphorus
Sour 3 parts of hydrogen calcium, 2 parts of composite mineral matter, 3 parts of B B-complex, 0.9 part of methionine, 0.9 part of lysine.
Comparative example 4
This comparative example and a kind of gene editing of embodiment 1 experiment mouse feed are essentially identical, and difference is, is walked in preparation method
The refining temperature of steam is 140-150 DEG C described in rapid upper S2, and other method is identical.
Application examples zoopery
By the experimental mouse after gene editing according to even group-division situations such as age in days, body weight, health status, it is divided into 7 experimental groups and 4
Individual control group, every group of 3 repetitions are each to repeat to include the experimental mouse after 10 gene editings, test in constant temperature, the experiment of constant humidity
Interior is unified to be carried out, and water, feeding are regularly fed during experiment（Each group difference feeding correspondence formula forage）, obtain each group experimental mouse
The death rate and reproductive survival rate are as shown in the table.
The embodiment of the present invention feeds the death rate reduction about 20-40% of experimental mouse compared with comparative example, and breeding potential improves about 20-
30%.Comparative example 1 changes the proportioning of dregs of beans, yeast hydrolyate, B B-complex, and comparative example 2 replaces yeast hydrolyate, battalion
Material collocation is supported not as the present invention enriches, rationally, experimental mouse is also significantly affected and digests and assimilates effect for feed；Comparative example 3
The composite mineral matter, B B-complex, methionine and lysine etc. are matched not within the scope of the present invention, it is impossible to realize the present invention
Synergistic action effect between each component, efficiency of feed utilization is low；The change of the preparation method of comparative example 4 will influence the feed
Property, influences the form of its interior active material, is had little effect to improving experimental mouse non-specific immunity, dead to experimental mouse
The improvement of rate and breeding potential is smaller.Found through Experimental comparison, the formula of embodiment 1 is the optimal proportion of the present invention, embodiment 2 ~ 3
Formula for the present invention more excellent proportioning；The formula and preparation method of gene editing experiment mouse feed of the present invention are raised compared to existing
Material and contrast feed, feed intake, survival rate and growth breeding effect to experimental mouse are all significantly improved, can be applicable.
Implementation of the invention described in detail above, still, the present invention are not limited to specific thin in above-mentioned embodiment
Section, in the range of the technology design of the present invention, can carry out a variety of simple variants to technical scheme, these simple changes
Type belongs to protection scope of the present invention.
Claims (7)
1. a kind of gene editing tests mouse feed, it is characterised in that include each component of following parts by weight：30-35 parts of corn,
10-15 parts of wheat, 15-20 parts of dregs of beans, crack rice 5-12 parts, 3-10 parts of fish meal, 1-5 parts of oil, 2-9 parts of yeast hydrolyate, stone flour
0.8-1.2 parts, 1-2 parts of calcium monohydrogen phosphate, 0.5-1 parts of composite mineral matter, 0.1-0.5 parts of B B-complex, methionine 0.1-0.2
Part, 0.1-0.2 parts of lysine.
2. a kind of gene editing experiment mouse feed according to claim 1, it is characterised in that by each of following parts by weight
Component is constituted：30-33 parts of corn, 13-15 parts of wheat, 19.5-20 parts of dregs of beans, crack rice 10-12 parts, 8-8.5 parts of fish meal, oil 2.5-
3 parts, 7.4-8.5 parts of yeast hydrolyate, 1.1-1.2 parts of stone flour, 1.0 parts of calcium monohydrogen phosphate, 0.6 part of composite mineral matter, compound dimension life
0.4 part of element, 0.2 part of methionine, 0.2 part of lysine.
3. a kind of gene editing experiment mouse feed according to claim 2, it is characterised in that by each of following parts by weight
Component is constituted：30 parts of corn, 15 parts of wheat, 20 parts of dregs of beans, crack rice 12 parts, 8 parts of fish meal, oil 3 parts, 8.4 parts of yeast hydrolyate, stone
1.2 parts of powder, 1 part of calcium monohydrogen phosphate, 0.6 part of composite mineral matter, 0.4 part of B B-complex, 0.2 part of methionine, 0.2 part of lysine.
4. a kind of gene editing experiment mouse feed according to any one of claim 1 ~ 3, it is characterised in that the yeast water
The preparation method for solving thing is as follows：Using fresh saccharomyces cerevisiae as raw material, formed through self-dissolving, enzymolysis, concentration, dry refine.
5. a kind of gene editing experiment mouse feed according to any one of claim 1 ~ 3, it is characterised in that the grandidierite
Mass percent in material shared by each element is：Copper 6%, iron 6%, manganese 1.3%, zinc 4.2%, selenium 0.3%, magnesium 1.5%, iodine 0.13%,
Cobalt 0.02%, surplus is carrier zeolite powder.
6. a kind of gene editing experiment mouse feed according to any one of claim 1 ~ 3, it is characterised in that the compound dimension
Raw element is two in vitamin A, vitamin C, vitamin D, vitamin E, vitamin K, calcium pantothenate, nicotinic acid, biotin or folic acid
Plant the mixture of the above.
7. gene editing described in claim 1 tests the preparation method of mouse feed, it is characterised in that comprise the following steps：S1.
Raw material is prepared：After corn, wheat, dregs of beans and crushing of cracking rice, it is sufficiently mixed with surplus stock；S2. modulation granulation：Will
The raw material input granulator mixed, pellet is made under 80-120 DEG C of steam；S3. feed is screened：It will make
Good pellet is screened, and retains particle diameter 11-13mm, and length is 30-40mm pellet；S4. nutritional surveillance and bag
Dress：Nutritive index, sanitary index, microbial indicators detected are carried out to the feed screened, carried out for standard compliant feed
Packaging, storage.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109123079A (en) * | 2018-09-27 | 2019-01-04 | 江苏省协同医药生物工程有限责任公司 | A kind of transgenic experiments mouse feed and preparation method thereof |
| CN111248151A (en) * | 2020-01-19 | 2020-06-09 | 江西中医药大学 | Construction method of experimental model for safety evaluation of tonifying traditional Chinese medicine |
| CN111528348A (en) * | 2020-06-02 | 2020-08-14 | 北京金道欣生物技术有限公司 | Laboratory mouse feed formula |
| CN113545423A (en) * | 2021-07-09 | 2021-10-26 | 江苏美迪森生物医药有限公司 | Genetic engineering laboratory mouse feed and preparation method thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102648734A (en) * | 2011-05-17 | 2012-08-29 | 北京华阜康生物科技股份有限公司 | Kk mouse compound feed |
| CN102783571A (en) * | 2011-05-17 | 2012-11-21 | 北京华阜康生物科技股份有限公司 | Genetically engineered mouse feed |
| CN105104883A (en) * | 2015-09-23 | 2015-12-02 | 江苏省协同医药生物工程有限责任公司 | Immunodeficiency mouse feed and preparation method thereof |
| CN105360663A (en) * | 2015-12-21 | 2016-03-02 | 中国农业科学院兰州畜牧与兽药研究所 | Feed special for SPF-stage inbred strain mice |
| CN105724765A (en) * | 2016-02-26 | 2016-07-06 | 山东深海生物科技股份有限公司 | Feed additive of penaeus vannamei and preparation method and application thereof |
-
2017
- 2017-05-27 CN CN201710390093.7A patent/CN107048048A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102648734A (en) * | 2011-05-17 | 2012-08-29 | 北京华阜康生物科技股份有限公司 | Kk mouse compound feed |
| CN102783571A (en) * | 2011-05-17 | 2012-11-21 | 北京华阜康生物科技股份有限公司 | Genetically engineered mouse feed |
| CN105104883A (en) * | 2015-09-23 | 2015-12-02 | 江苏省协同医药生物工程有限责任公司 | Immunodeficiency mouse feed and preparation method thereof |
| CN105360663A (en) * | 2015-12-21 | 2016-03-02 | 中国农业科学院兰州畜牧与兽药研究所 | Feed special for SPF-stage inbred strain mice |
| CN105724765A (en) * | 2016-02-26 | 2016-07-06 | 山东深海生物科技股份有限公司 | Feed additive of penaeus vannamei and preparation method and application thereof |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109123079A (en) * | 2018-09-27 | 2019-01-04 | 江苏省协同医药生物工程有限责任公司 | A kind of transgenic experiments mouse feed and preparation method thereof |
| CN111248151A (en) * | 2020-01-19 | 2020-06-09 | 江西中医药大学 | Construction method of experimental model for safety evaluation of tonifying traditional Chinese medicine |
| CN111528348A (en) * | 2020-06-02 | 2020-08-14 | 北京金道欣生物技术有限公司 | Laboratory mouse feed formula |
| CN113545423A (en) * | 2021-07-09 | 2021-10-26 | 江苏美迪森生物医药有限公司 | Genetic engineering laboratory mouse feed and preparation method thereof |
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Application publication date: 20170818 |
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