CN111109466B - High-calcium high-potassium broussonetia papyrifera leaf feed additive and preparation method and application thereof - Google Patents
High-calcium high-potassium broussonetia papyrifera leaf feed additive and preparation method and application thereof Download PDFInfo
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Abstract
The invention discloses a high-calcium high-potassium broussonetia papyrifera leaf feed additive which consists of a broussonetia papyrifera leaf fermentation product, a mixture of calcium formate and potassium diformate; wherein, the mass of the broussonetia papyrifera leaf fermentation product accounts for 30-90%, and the mass of the mixture of calcium formate and potassium diformate accounts for 10-70%. Compared with the prior art, the invention utilizes calcium formate and potassium diformate to release formic acid through biochemical action in the body of the laying hen, can pass through bacterial cell walls, effectively reduces the pH value in the gastrointestinal tract, plays a role in maintaining proper acid in the intestinal tract, is beneficial to activating pepsin sources of the laying hen, improves the digestibility of nutrients of feed for the laying hen and increases energy utilization. The broussonetia papyrifera leaf fermentation product is rich in trace elements such as broussonetia papyrifera protein, calcium, phosphorus, zinc, iron, manganese, copper and the like and various amino acids, can obviously improve the growth performance of the laying hen, reduce the morbidity of the laying hen, improve the organism of the laying hen and improve the laying rate of the laying hen.
Description
Technical Field
The invention belongs to the field of feed additives, and particularly relates to a high-calcium high-potassium broussonetia papyrifera leaf feed additive, and a preparation method and application thereof.
Background
With the continuous improvement of the living standard of people, people begin to pursue to eat safely and healthily, and safe and healthy consumption is becoming the subject of consumption of people in China. The development of green health food is an important way for improving the quality and safety of agricultural products, the consumption demand of chicken products in China continuously and rapidly increases, and the consumption of eggs and chicken per person respectively increases a lot in the past 10 years. The average consumption of eggs in China is far above the average worldwide level. Chicken products have become the most important source of animal protein after pork. At present, environmental pollution, severe epidemic situation, inadequacy of related standards (veterinary drugs, feeds and foods) and the like caused by intensive and large-scale laying hen industry in China become serious problems endangering the health of people and the survival of the laying hen industry.
The chicken feed is a guarantee of the chicken raising industry, and the quality of the chicken feed can seriously influence the growth rate of chickens, the quality of chicken meat and the chicken raising cost; especially, the quality of the chicken feed can greatly influence the body performance of the laying hens and the egg yield. In view of this, in the prior art, there are a great deal of technical literature, research is conducted on chicken feeds and additives in chicken feeds, so that rich nutrition is provided for laying hens, the body performance of the laying hens is ensured to be better, the egg yield is improved, and in order to avoid the traditional chicken feeds or chicken feed additives, artificial synthetic compositions, antibiotic drugs and the like are adopted, the research on fermentation products of green plants of broussonetia papyrifera leaves is the key point of the current research.
Disclosure of Invention
The invention aims to solve the technical problem of providing a high-calcium high-potassium broussonetia papyrifera leaf feed additive.
The invention also solves the technical problem of providing a preparation method of the additive.
The technical problem to be further solved by the invention is to provide the application of the additive.
In order to solve the technical problems, the invention discloses a high-calcium high-potassium broussonetia papyrifera leaf feed additive which consists of a broussonetia papyrifera leaf fermentation product, a mixture of calcium formate and potassium diformate.
Wherein, the mass ratio of the broussonetia papyrifera leaf fermentation product to the mixture of calcium formate and potassium diformate is (30% -90%): (10% -70%).
Preferably, the mass ratio of the broussonetia papyrifera leaf fermentation product to the mixture of calcium diformate and potassium diformate is (40% -80%): (20% -60%); more preferably, the mass ratio of the broussonetia papyrifera leaf fermentation product to the mixture of calcium diformate potassium formate is 7:3.
Wherein, the paper mulberry living environment is loose, and the paper mulberry can be planted on a large-area marginal land with the annual rainfall of more than 300mm, the lowest air temperature of less than 25 ℃ and the saline alkali content of less than 6 per mill, and the paper mulberry can be planted in the same year. The planting characteristics of the paper mulberry are not easy to generate plant diseases and insect pests, and the paper mulberry is a green organic feed without pesticides and hormones. The paper mulberry leaf crude protein content is 26.69%, the trace elements such as Ca, P, zn, fe, mn, cu and the like and the amino acid content are rich, and the paper mulberry leaf fermentation product is rich in nutrition and added into chicken feed, so that the paper mulberry leaf fermentation product can be used for enhancing the health care effect of the chicken feed, reducing the morbidity of chickens, improving the organism and increasing the laying rate.
Calcium formate and potassium diformate are biochemically acted in the layers to release formic acid, which can pass through bacterial cell walls to effectively reduce the pH value in the gastrointestinal tract, and maintain proper acid in the intestinal tract, which is beneficial to activating pepsin source of the layers, improving the digestibility of the layer feed nutrients and increasing energy utilization. Formate anions decompose bacterial cell wall proteins outside the cell wall, play a role in sterilizing and inhibiting bacteria, such as salmonella, and reduce anaerobic bacteria in chyme in each section of the digestive tract. Thereby improving the organism of the laying hen and improving the organism immunity.
The preparation method of the broussonetia papyrifera leaf fermentation product comprises the following steps:
(1) Cutting broussonetia papyrifera leaves, putting the broussonetia papyrifera leaves into a grinder, grinding the broussonetia papyrifera leaves, sieving the broussonetia papyrifera leaves, soaking the broussonetia papyrifera leaves into a Tween aqueous solution, adding lactobacillus for fermentation (the used lactobacillus is produced by Shandong micro-high biotechnology Co., ltd.), obtaining fermentation liquor, filtering the fermentation liquor, and drying filter residues at 60-80 ℃ for later use;
(2) Adding cellulase into the filter residue obtained in the step (1), and regulating the pH value to be neutral by hydrochloric acid for enzymolysis;
(3) Adding active yeast into the enzymolysis liquid obtained in the step (2), fermenting to obtain fermentation liquid, filtering, and drying filter residues to obtain a paper mulberry leaf fermentation product.
In the step (1), the mesh number of the broussonetia papyrifera leaves is 30-80 mesh; the Tween is Tween 80; the concentration of the Tween aqueous solution is 30-50 g/L; the ratio of the broussonetia papyrifera leaves to the tween aqueous solution is 1:6-8; the soaking condition is that soaking is carried out for 3-5 hours at 80-90 ℃, and the drying temperature is 60-80 ℃.
In the step (1), the concentration of the lactobacillus is 40-50 g/L, and the fermentation condition is 30-40 ℃ for sealing fermentation for 10-12 h.
In the step (2), the enzyme activity of the cellulase is 50000U/g; the addition amount of the cellulase is 2g/L; the enzymolysis temperature is 45-55 ℃, the enzymolysis pH is neutral pH, and the enzymolysis time is 10-15 h.
Wherein the enzyme activity is defined as (1 g of enzyme powder (or 1ml of enzyme liquid) is used for hydrolyzing a substrate (filter paper, CMC, absorbent cotton or salicin) at 50 ℃ and pH 4.8 for 1min, and the enzyme amount for generating 1 mu g of glucose is 1 enzyme activity unit).
In step (3), the active yeast comprises about 200 million live yeast cells per gram; the addition amount of the active yeast is 6-10 g/L; the fermentation is carried out for 12-15 h at normal temperature.
Wherein, the mixture of calcium formate and potassium diformate comprises the following components in percentage by mass:
20 to 80 percent of calcium formate
20-80% of potassium diformate.
Preferably, the calcium formate is 50% and the potassium diformate is 50%.
The preparation method of the mixture of calcium formate and potassium diformate comprises the step of uniformly mixing the calcium formate and the potassium diformate in a physical mixing mode.
The preparation method of the high-calcium high-potassium broussonetia papyrifera leaf feed additive adopts a physical mixing mode to uniformly mix the broussonetia papyrifera leaf fermentation product, calcium formate and potassium diformate.
The application of the high-calcium high-potassium broussonetia papyrifera leaf feed additive in chicken feed is also within the protection scope of the invention; preferably, the chicken feed is a layer feed.
Wherein the addition amount of the additive in the chicken feed is 1-10% of the dry weight of the whole feed, and preferably 5-10%.
The application method is that the feed additive is added into basic feed of laying hens.
The laying hen feed additive prepared by combining the broussonetia papyrifera leaf fermentation product and the mixture of the calcium diformate and the potassium diformate according to a certain proportion can promote laying of laying hens and improve the weight and quality of eggs; simultaneously, the pH value obtained in the gastrointestinal tract of the laying hen is effectively reduced, and the effect of maintaining proper acid in the intestinal tract is achieved. The pepsin source of the laying hens is conveniently activated, the nutrient digestion rate of the feed of the laying hens is improved, the immunity of the laying hens can be obviously improved, the feeding amount of the laying hens is increased, and the weight of the laying hens is further improved.
The beneficial effects are that: compared with the prior art, the invention has the following advantages:
(1) In the invention, calcium formate and potassium diformate are biochemically acted in the body of the laying hen to release formic acid which can pass through the cell wall of bacteria, so that the pH value obtained in the gastrointestinal tract is effectively reduced, the effect of maintaining proper acid in the intestinal tract is achieved, the pepsin source of the laying hen is favorably activated, the digestibility of the nutrients of the feed of the laying hen is improved, and the energy utilization is increased. The formate anions decompose bacterial cell wall proteins outside the cell wall, play a role in sterilizing and inhibiting bacteria, such as salmonella, and reduce anaerobic bacteria in chyme of each section of the digestive tract, thereby improving the organism of the laying hens and enhancing the organism immunity.
(2) According to the invention, the content of lactic acid bacteria and lactic acid can be increased by anaerobic fermentation of the broussonetia papyrifera leaf fermentation product through lactic acid bacteria. The pretreatment method for soaking the broussonetia papyrifera leaves in the Tween-80 aqueous solution changes the permeability of cell membranes of the broussonetia papyrifera leaves, improves enzymolysis effect, increases the protein content of fermentation products through twice fermentation, and the broussonetia papyrifera leaves fermentation products are rich in trace elements such as broussonetia papyrifera proteins, calcium, phosphorus, zinc, iron, manganese, copper and the like and various amino acids, so that the growth performance of the laying hen can be obviously improved, the morbidity of the laying hen can be reduced, the body of the laying hen can be improved, and the laying rate of the laying hen can be improved.
(3) The invention belongs to biological products, and is green, environment-friendly and pollution-free.
Detailed Description
The invention will be better understood from the following examples. However, it will be readily appreciated by those skilled in the art that the description of the embodiments is provided for illustration only and should not limit the invention as described in detail in the claims.
Example 1: preparation of additives
(1) Cutting broussonetia papyrifera leaves, putting the broussonetia papyrifera leaves into a grinder, grinding the broussonetia papyrifera leaves, sieving the broussonetia papyrifera leaves with a 70-mesh sieve, adding a tween-80 aqueous solution (the concentration of the tween-80 aqueous solution is 50 g/L) according to the ratio of the broussonetia papyrifera leaves to the tween-80 aqueous solution of 1:7, soaking the broussonetia papyrifera leaves for 5 hours at the temperature of 85 ℃, adding 50g/L of lactobacillus, sealing and fermenting the obtained sample at the temperature of 35 ℃ for 12 hours, and drying the obtained sample at the temperature of 80 ℃ for later use;
(2) Adding 50000U/g cellulase with the enzyme activity to the filter residue obtained in the step (1) for enzymolysis, wherein the addition amount of the cellulase is 2g/L, the enzymolysis temperature is 50 ℃, the pH of the enzymolysis is neutral pH, and the enzymolysis time is 15 hours;
(3) Adding active yeast into the enzymolysis liquid obtained in the step (2), wherein the addition amount of the active yeast is 10g/L; the fermentation is carried out for 15 hours at normal temperature to obtain fermentation liquor, filtering is carried out, and filter residues are dried, thus obtaining the paper mulberry leaf fermentation product.
(4) Mixing 70% of broussonetia papyrifera leaf fermentation product and 30% of calcium diformate by physical method (wherein the calcium diformate comprises 50% of calcium formate and 50% of potassium diformate) to obtain the high-calcium high-potassium broussonetia papyrifera leaf feed additive.
Example 2: preparation of additives
(1) Cutting broussonetia papyrifera leaves, putting the broussonetia papyrifera leaves into a grinder, grinding the broussonetia papyrifera leaves, sieving the broussonetia papyrifera leaves with a 70-mesh sieve, adding a tween-80 aqueous solution (the concentration of the tween-80 aqueous solution is 50 g/L) into the broussonetia papyrifera leaves at a ratio of 1:6, soaking the broussonetia papyrifera leaves in the broussonetia papyrifera leaves at a temperature of 85 ℃ for 3 hours, adding 40 g/L of lactobacillus, sealing and fermenting the obtained product at a temperature of 35 ℃ for 10 hours, and drying the obtained sample at a temperature of 70 ℃ for later use;
(2) Adding 50000U/g cellulase with the enzyme activity to the filter residue obtained in the step (1) for enzymolysis, wherein the addition amount of the cellulase is 2g/L, the enzymolysis temperature is 50 ℃, the pH of the enzymolysis is neutral pH, and the enzymolysis time is 13h;
(3) Adding active yeast into the enzymolysis liquid obtained in the step (2), wherein the addition amount of the active yeast is 8 g/L; the fermentation is carried out for 14h at normal temperature to obtain fermentation liquor, filtering is carried out, and filter residues are dried, thus obtaining the paper mulberry leaf fermentation product.
(4) The paper mulberry leaf fermentation product 60% and the calcium diformate 40% are physically mixed together (wherein the calcium diformate comprises 60% of calcium formate and 40% of potassium diformate), so that the paper mulberry leaf feed additive with high calcium and high potassium is obtained.
Comparative example 1: rather than using lactic acid bacteria
In the same manner as in example 1, only the steps of (1) were changed to shredding the broussonetia papyrifera leaves, pulverizing in a pulverizer, sieving with a 70 mesh sieve, adding tween-80 aqueous solution according to a ratio of broussonetia papyrifera leaves to tween aqueous solution of 1:7, soaking at 85deg.C for 5 hr, fermenting at 35deg.C for 12 hr under sealed condition without adding lactobacillus, and oven drying the obtained sample at 80deg.C for use
Comparative example 2: without using aqueous Tween 80
In the same way as in example 1, only the paper mulberry leaves in the step (1) are cut up, put into a pulverizer for pulverization, and after passing through a 70-mesh sieve, pure water is added according to the ratio of paper mulberry leaves to pure water of 1:6, and after soaking treatment for 3 hours at 85 ℃, 40 g/L of lactobacillus is added, and sealed fermentation is carried out for 10 hours at 35 ℃, and the obtained sample is dried at 70 ℃ for later use;
comparative example 3: fermenting only once
As in example 1, the residue obtained in step (1) was left for 15 hours with the modification of step (2) alone.
Comparative example 4: does not contain a mixture of calcium diformate and potassium diformate
As in example 1, only the modification of step (2) was to physically mix together 100% of the fermentation product of broussonetia papyrifera leaves (0% of calcium formate).
Example 3: application of additive in feed
The additive prepared in example 1 was added to highly concentrated chicken feed from the company of the Suaeda salsa feed limited in a proportion of 5g of the high-calcium and high-potassium broussonetia papyrifera leaf feed additive for laying hens per 100g of the commercially available laying hen feed, pouring the mixture into a feed stirrer, stirring and mixing uniformly, and taking out the uniformly stirred and mixed materials, thereby obtaining the laying hen feed meeting the requirements of the invention.
Example 4:
the additive prepared in the example 1 is added into the highly concentrated chicken feed of Jiangsu Su Suan He feed Limited company, 9g of the high-calcium high-potassium Broussonetia papyrifera leaf feed additive for laying hens is mixed into 100g of the commercial laying hen feed, the mixture is poured into a feed stirrer, the mixture is stirred and mixed uniformly, and the stirred and mixed uniformly material is taken out, so that the laying hen feed meeting the requirements of the invention is produced.
Example 5:
the additive prepared in example 1 was added to highly concentrated chicken feed from the company of the Suaeda salsa feed limited in a proportion of 10g of the high-calcium high-potassium broussonetia papyrifera leaf feed additive for laying hens per 100g of the commercially available laying hen feed, pouring the mixture into a feed stirrer, stirring and mixing uniformly, and taking out the uniformly stirred and mixed materials, thereby obtaining the laying hen feed meeting the requirements of the invention.
Example 6:
the additive prepared in example 1 was added to highly concentrated chicken feed from the company of the Suaeda salsa feed limited in a proportion of 8g of the high-calcium and high-potassium broussonetia papyrifera leaf feed additive for laying hens per 100g of the commercially available laying hen feed, pouring the mixture into a feed stirrer, stirring and mixing uniformly, and taking out the uniformly stirred and mixed materials, thereby obtaining the laying hen feed meeting the requirements of the invention.
Example 7:
the additive prepared in example 1 was added to highly concentrated chicken feed from the company of the Suaeda salsa feed limited in a proportion of 6g of the high-calcium and high-potassium broussonetia papyrifera leaf feed additive for laying hens per 100g of the commercially available laying hen feed, pouring the mixture into a feed stirrer, stirring and mixing uniformly, and taking out the uniformly stirred and mixed materials, thereby obtaining the laying hen feed meeting the requirements of the invention.
Example 8:
the additive prepared in example 1 was replaced by the additive prepared in example 2 as in example 5.
Comparative example 5:
the additives prepared in example 1 were replaced with the additives prepared in comparative examples 1 to 4, respectively, as in example 5.
Example 9:
feeding experiments are carried out, and 500 normal laying hens about 200 days old are randomly and averagely divided into 50 chickens in 10 groups of experiment group 1, experiment group 2, experiment group 3, experiment group 4, experiment group 5, experiment group 6, control group 1, control group 2, control group 3, control group 4 and control group 5.
The 10 groups of experiments were:
experiment group 1: the feed is the feed for laying hens obtained in the embodiment 3;
experiment group 2: the feed is the feed for laying hens obtained in the embodiment 4;
experiment group 3: the feed is the feed for laying hens obtained in the embodiment 5;
experiment group 4: the feed is the feed for laying hens obtained in the embodiment 6;
experimental group 5: the feed is the feed for laying hens obtained in the embodiment 7;
experiment group 6: the feed is the feed for laying hens obtained in the embodiment 8;
control group 1: the feed is common commercial laying hen feed;
control group 2: the feed was a chicken feed prepared in comparative example 5 using comparative example 1 as a raw material;
control group 3: the feed is the chicken feed prepared by taking the comparative example 2 as the raw material in the comparative example 5;
control group 4: the feed is the chicken feed prepared by taking the comparative example 3 as the raw material in the comparative example 5;
control group 5: the feed is the chicken feed prepared by taking the comparative example 4 as the raw material in the comparative example 5;
the feed was sufficient daily and data statistics were performed on day 16, day 32, day 48, and day 64, respectively.
Egg yield = number of laying hens laying eggs (cumulative data over 16 days)/total number of laying hens
Mortality = number of layers dead (cumulative data over 16 days)/total number of layers
Average egg weight = weight of each layer (average weight of eggs over 16 days)
Table 1 shows egg weight comparisons of egg production, mortality, and production of eggs from experimental group 1, experimental group 2, experimental group 3, experimental group 4, experimental group 5, experimental group 6, and control groups.
TABLE 1
From the above table, it can be seen that: examples 3 to 8 can increase the content of lactic acid bacteria and lactic acid by anaerobic fermentation with lactic acid bacteria as compared with comparative example 1; compared with comparative example 2, it can be seen that the pretreatment method of soaking Broussonetia papyrifera leaves in tween-80 aqueous solution in examples 3-8 changes the permeability of cell membranes of Broussonetia papyrifera leaves, and improves enzymolysis effect; compared with comparative example 3, the protein content of the fermentation product is increased by twice fermentation in examples 3-8, and the fermentation product of the broussonetia papyrifera leaves is rich in trace elements such as broussonetia papyrifera protein, calcium, phosphorus, zinc, iron, manganese, copper and the like and various amino acids, so that the growth performance of the laying hen can be obviously improved, the morbidity of the laying hen can be reduced, the organism of the laying hen can be improved, and the laying rate of the laying hen can be increased; compared with the feed additive without adding calcium formate and potassium diformate in the comparative example 4, the calcium formate and the potassium diformate in the feed additive components in the examples 3-8 are biochemically acted in the body of the laying hen to release formic acid which can penetrate through the cell wall of bacteria, so that the pH value in the gastrointestinal tract is effectively reduced, the effect of maintaining proper acid in the intestinal tract is achieved, the pepsin source of the laying hen is favorably activated, the nutrient digestibility of the feed of the laying hen is improved, and the energy utilization is increased.
Overall, the present invention reduced the mortality of the layers and increased the weight of the eggs laid by the layers compared to the comparative examples, with the effect of example 5 (experimental group 3) being the best.
The invention provides a high-calcium and high-potassium broussonetia papyrifera leaf feed additive, a preparation method and an application thought and method thereof, and particularly the method and the way for realizing the technical scheme are a plurality of preferred embodiments of the invention, and it is pointed out that a plurality of improvements and modifications can be made by one of ordinary skill in the art without departing from the principle of the invention, and the improvements and modifications are also regarded as the protection scope of the invention. The components not explicitly described in this embodiment can be implemented by using the prior art.
Claims (9)
1. The high-calcium high-potassium broussonetia papyrifera leaf feed additive is characterized by comprising a broussonetia papyrifera leaf fermentation product, calcium formate and potassium diformate; the mixture of calcium formate and potassium diformate comprises the following components in percentage by mass: 50% of calcium formate and 50% of potassium diformate;
wherein, the mass of the broussonetia papyrifera leaf fermentation product accounts for 30% -90%, and the mass of the mixture of calcium formate and potassium diformate accounts for 10% -70%;
the preparation method of the broussonetia papyrifera leaf fermentation product comprises the following steps:
(1) Soaking Broussonetia papyrifera leaves in Tween aqueous solution, adding lactobacillus for fermentation to obtain fermentation liquid, filtering, and drying the filter residue; the concentration of the Tween aqueous solution is 30-50 g/L; the ratio of the broussonetia papyrifera leaves to the tween aqueous solution is 1:6-8; fermenting under sealed condition at 30-40 deg.c for 10-12 hr;
(2) Adding cellulase into the filter residue obtained in the step (1) for enzymolysis;
(3) Adding active yeast into the enzymolysis liquid obtained in the step (2), fermenting to obtain fermentation liquid, filtering, and drying filter residues to obtain a broussonetia papyrifera leaf fermentation product; the fermentation is carried out for 12-15 h at normal temperature.
2. The high-calcium high-potassium broussonetia papyrifera leaf feed additive according to claim 1, wherein in the step (1), the mesh number of broussonetia papyrifera leaves is 30-80 mesh; the Tween is Tween 80; the soaking condition is that soaking is carried out for 3-5 hours at 80-90 ℃.
3. The high-calcium high-potassium broussonetia papyrifera leaf feed additive according to claim 1, wherein in the step (1), the concentration of the lactobacillus is 40-50 g/L.
4. The high-calcium high-potassium broussonetia papyrifera leaf feed additive according to claim 1, wherein in the step (2), the enzyme activity of the cellulase is 50000U/g; the addition amount of the cellulase is 2g/L; the enzymolysis temperature is 45-55 ℃, the enzymolysis pH is neutral pH, and the enzymolysis time is 10-15 h.
5. The high calcium high potassium broussonetia papyrifera leaf feed additive of claim 1 wherein in step (3), the active yeast contains about 200 million active yeast cells per gram; the addition amount of the active yeast is 6-10 g/L.
6. The high-calcium high-potassium broussonetia papyrifera leaf feed additive according to claim 1, wherein the preparation method of the mixture of calcium formate and potassium diformate is to mix the calcium formate and the potassium diformate evenly by adopting a physical mixing mode.
7. The method for preparing the high-calcium high-potassium broussonetia papyrifera leaf feed additive according to claim 1, wherein the physical mixing mode is adopted to mix the broussonetia papyrifera leaf fermentation product, calcium formate and potassium diformate evenly.
8. The use of the high-calcium high-potassium broussonetia papyrifera leaf feed additive of claim 1 in chicken feed.
9. The use according to claim 8, wherein the additive is added to the chicken feed in an amount of 1% to 10% of the dry weight of the whole feed.
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|---|---|---|---|
| CN201911308110.3A CN111109466B (en) | 2019-12-18 | 2019-12-18 | High-calcium high-potassium broussonetia papyrifera leaf feed additive and preparation method and application thereof |
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| CN201911308110.3A CN111109466B (en) | 2019-12-18 | 2019-12-18 | High-calcium high-potassium broussonetia papyrifera leaf feed additive and preparation method and application thereof |
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| CN111109466A CN111109466A (en) | 2020-05-08 |
| CN111109466B true CN111109466B (en) | 2023-08-22 |
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| CN109892494A (en) * | 2019-03-27 | 2019-06-18 | 天水市畜牧技术推广站 | One kind improving laying hen immune performance feed and preparation method thereof as raw material to hybridize paper mulberry |
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| CN109247454A (en) * | 2018-11-19 | 2019-01-22 | 天水市畜牧技术推广站 | The feed and preparation method thereof of chicken antioxidative functional is significantly improved using paper mulberry as raw material |
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