CN103396210A - Stichopus japonicus fertilizer, and preparation and application methods thereof - Google Patents

Stichopus japonicus fertilizer, and preparation and application methods thereof Download PDF

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CN103396210A
CN103396210A CN2013103090927A CN201310309092A CN103396210A CN 103396210 A CN103396210 A CN 103396210A CN 2013103090927 A CN2013103090927 A CN 2013103090927A CN 201310309092 A CN201310309092 A CN 201310309092A CN 103396210 A CN103396210 A CN 103396210A
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stichopus japonicus
fertilizer
vitamin
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CN103396210B (en
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沈建明
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Suzhou Yangcheng Lake Modern Agriculture Industrial Park Special Aquaculture Co Ltd
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Suzhou Yangcheng Lake Modern Agriculture Industrial Park Special Aquaculture Co Ltd
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Abstract

The invention relates to a Pseudobagrus fulvidraco feed. The invention is characterized in that the Pseudobagrus fulvidraco feed comprises the following raw materials in parts by weight: 0.5-0.8 part of silicate bacterium, 1-3 parts of Streptococcus faecalis, 0.6-0.8 part of Trichoderma, 2-5 parts of Rhodopseudomonas palustris, 0.3-0.5 part of microzyme, 50-60 parts of fishbone dust, 65-70 parts of vinegar residue powder, 50-55 parts of waste liquor precipitate, 40-60 parts of rice bran, 12-14 parts of brown sugar, 10-14 parts of table salt, 80-85 parts of pig manure, 45-55 parts of pork abattoir waste, 60-65 parts of aquatic, 12-16 parts of calcium bicarbonate, 14-17 parts of urea, 1-3 parts of ferric citrate, 0.6-0.8 part of vitamin B1, 0.2-0.6 part of vitamin B12, 20-30 parts of pepper and the like. A comparison shows that the composite microbial fertilizer can promote quick growth of stichopus japonicus, increase the EPA content, reduce the fish diseases and the amount of fish dying of illness, and increase the beneficial microbes in the water body, thereby enhancing the final quality and grade.

Description

A kind of stichopus japonicus fertilizer and preparation thereof, using method
Technical field
The present invention relates to the feed of aquaculture, especially a kind of stichopus japonicus fertilizer.
Background technology
Sea cucumber is as a class precious marine product, plays an important role providing aspect food, medicine material, nutrition and protective foods.Stichopus japonicus (Apostichopus japonicus) is best, distribute the widest, a maximum kind of output of quality in domestic beche-de-mer, and it is coastal that its culturing area mainly is distributed in China Liaoning, Hebei, Shandong and Along North Jiangsu.In the 80's of 20th century, the artificial breeding technique of China stichopus japonicus has had breakthrough progress, has formed so far a cover than more complete production technology system.The apostichopus japonicus culture area constantly enlarges, and the apostichopus japonicus culture area in Shandong and Liaoning all surpasses 30,000 m2, and the output value is over 10,000,000,000 yuan.But in recent years, the problems such as the extensive disease of culturing stichopus japonicus, drug residue and deterioration of strains constantly occur.The seed problem is the key of stichopus japonicus industry sustainable and healthy development.The holothurian pond aquaculture development was rapid in recent years, but the cultural technique level is uneven, and it is huge that cultured output differs, and most of pond culture output is very low, and some Yield Fish Culture Ponds per unit area yields reach 3000kg/hm 2Above.Process is to the summary of pool holothurian culture practical experience for many years, it is mainly that existing holothurian pond standard is low, cultural method is lack of standardization, many modern technique measures are not universal that discovery causes the reason of above-mentioned phenomenon, existing single type fish guano and compound fish guano also have a common shortcoming, that can not be killed harmful bacteria, worm exactly, therefore, lack the effectively effect of control of fish disease.By the compound fish guano of microorganism, can increase the content of probiotics in fishpond, effectively reduce the generation of fish diseases, about being exclusively used in this complex microorganism fish guano, also not report at present.
Summary of the invention
One of purpose of the present invention is achieved through the following technical solutions:
a kind of stichopus japonicus fertilizer, it is characterized in that, its raw material comprises the composition of following weight ratio: bacillus subtilis bacteria powder 2-4 part, enzymatic microorganism 1-4 part, solid photosynthetic bacterium 0.5-0.9 part, bacteriophagic Bdellovibrio 0.8-1.2 part, VITAMIN addition material 0.2-0.6 part, mineral substance addition material 10-16 part, sequestrant 0.2-0.4 part, Herba Eichhorniae 60-80 part, edible fungi residue 50-80 part, vinasse 100-110 part, fishbone powder 40-60 part, rice bran 70-80 part, brown sugar 10-16 part, kongpo monkshood root 45-55 part, Semen Momordicae 40-50 part, Stellera chamaejasme L. 1-15 part, hops 5-10 part, matrine 5-10 part, Golden Larch Bark 10-15 part, Chinese prickly ash 20-30 part, ginkgo kind skin 20-30 part, Fructus Cnidii 20-30 part, Cortex Meliae 10-30 part, Rangooncreeper Fruit 15-25 part, Turkey-galls 1-15 part, Root of Pubescent Holly 5-15 part, Seed of Chinese Soapberry exosper 55-60 part, fructus ulmi 10-15 part, camphor 5-15 part.
Described VITAMIN addition material component recipe ratio is: vitamin A, C, E mix with the weight ratio of 2:3:5.
Described mineral substance addition material is potassium primary phosphate 20-25%, monocalcium phosphate 10-15%, ferrous sulfate 2-5%, zinc sulfate 1-2%, manganous sulfate 0.8-1%, borax 0.3-0.5%, rose vitriol 0.03-0.06%, rare earth 0.2-0.6%, and all the other add salt to 100%.
To be sodium humate and EDTA mix with the weight ratio of 1:1-2 described sequestrant.
A kind of preparation method of stichopus japonicus fertilizer comprises the following steps:
(1) at first Herba Eichhorniae, edible fungi residue, vinasse are dried in the sun to water content below 40%, then according to described ratio, add fishbone powder, rice bran, brown sugar, fully stir, mix;
(2) in said mixture, according to described ratio, add bacillus subtilis bacteria powder and the enzymatic microorganism of having dissolved, then above-mentioned substance is inserted to mixing corruption in pond and macerate, plastics film on upper cap, carry out fermenting in advance in 3-5 days; Then plastics film is thrown off, and stir, allow mixture contact oxygen, temperature gradually raises, from normal temperature to 45 ℃, keep continuing turning after 2-3 days, then be raised to 60 ℃ from 45 ℃, the hold-time is 3-5 days, then cool the temperature to normal temperature, keep 7-10 days, until slaking is fully fermented complete;
(3) solid photosynthetic bacterium, bacteriophagic Bdellovibrio are dissolved in water, join in said mixture, stir;
(4) by above-mentioned fermented product, air dry in the sun, make moisture content of material less than 25%, add kongpo monkshood root 45-55 part, Semen Momordicae 40-50 part, Stellera chamaejasme L. 1-15 part, hops 5-10 part, matrine 5-10 part, Golden Larch Bark 10-15 part, Chinese prickly ash 20-30 part, ginkgo kind skin 20-30 part, Fructus Cnidii 20-30 part, Cortex Meliae 10-30 part, Rangooncreeper Fruit 15-25 part, Turkey-galls 1-15 part, Root of Pubescent Holly 5-15 part, Seed of Chinese Soapberry exosper 55-60 part, fructus ulmi 10-15 part, then camphor 5-15 part pulverizes, pulverize fineness for passing through without screen pulverizer, after pulverizing, in material, add the mineral substance addition material, sequestrant and VITAMIN addition material, mix and obtain microbial fish guano.;
The using method of stichopus japonicus fertilizer is characterized in that: throw something and feed: in March in spring ~ May, should manually throw something and feed, do not throw something and feed July ~ September, and about mid-September, water temperature is fallen 18 ℃, enters stichopus japonicus and grow the thermophilic phase, strengthen daily ration, feeding quantity.Below 5 ℃, substantially do not ingest and stop throwing something and feeding.
The invention has the beneficial effects as follows:
(1) added that fish produce needed organism and inorganic mineral, trace element can provide comprehensive nutrition to the growth of fish;
(2) add subtilis, enzymatic microorganism and bacteriophagic Bdellovibrio, can kill the harmful bacteria in fishpond, suppressed the breeding growth of pathogenic bacteria in water body, reduced the generation of disease, improved the yield and quality of fish.
(3) added the solid photosynthetic bacterium, the toxic substances such as nitrite in water body, sulfide of can degrading, and can restrain oneself the organic waste water of high density, the poisonous substances such as phenol, cyanogen are had and necessarily stand and capacity of decomposition, have stronger decomposition and inversion ability, reduce the accumulation of toxic substance in the fish body.
(4) these mushrooms that add, can continue breeding in water body, enlarge survival rate, the balance of remaining microorganisms in water is had a very important role, and can regulate and adjust microecological balance in fish body enteron aisle, can also decomposer in the Harmful Residue, strengthen the fingerling disease resistance.
(5) the Chinese medicine sterilizing composition is little on natural enemy of fish impact, helps to keep ecotope, and is more friendly to environment, to dactylogyrider, Trichodina desinsection better, meet nuisanceless, green production requirement.
Embodiment
Below will be described in detail the preferred embodiments of the present invention; Should be appreciated that preferred embodiment is only for the present invention is described, rather than in order to limit the scope of the invention.
a kind of stichopus japonicus fertilizer, it is characterized in that, its raw material comprises the composition of following weight ratio: bacillus subtilis bacteria powder 2-4 part, enzymatic microorganism 1-4 part, solid photosynthetic bacterium 0.5-0.9 part, bacteriophagic Bdellovibrio 0.8-1.2 part, VITAMIN addition material 0.2-0.6 part, mineral substance addition material 10-16 part, sequestrant 0.2-0.4 part, Herba Eichhorniae 60-80 part, edible fungi residue 50-80 part, vinasse 100-110 part, fishbone powder 40-60 part, rice bran 70-80 part, brown sugar 10-16 part, kongpo monkshood root 45-55 part, Semen Momordicae 40-50 part, Stellera chamaejasme L. 1-15 part, hops 5-10 part, matrine 5-10 part, Golden Larch Bark 10-15 part, Chinese prickly ash 20-30 part, ginkgo kind skin 20-30 part, Fructus Cnidii 20-30 part, Cortex Meliae 10-30 part, Rangooncreeper Fruit 15-25 part, Turkey-galls 1-15 part, Root of Pubescent Holly 5-15 part, Seed of Chinese Soapberry exosper 55-60 part, fructus ulmi 10-15 part, camphor 5-15 part.
Described VITAMIN addition material component recipe ratio is: vitamin A, C, E mix with the weight ratio of 2:3:5.
Described mineral substance addition material is potassium primary phosphate 20-25%, monocalcium phosphate 10-15%, ferrous sulfate 2-5%, zinc sulfate 1-2%, manganous sulfate 0.8-1%, borax 0.3-0.5%, rose vitriol 0.03-0.06%, rare earth 0.2-0.6%, and all the other add salt to 100%.
To be sodium humate and EDTA mix with the weight ratio of 1:1-2 described sequestrant.
A kind of preparation method of stichopus japonicus fertilizer comprises the following steps:
(1) at first Herba Eichhorniae, edible fungi residue, vinasse are dried in the sun to water content below 40%, then according to described ratio, add fishbone powder, rice bran, brown sugar, fully stir, mix;
(2) in said mixture, according to described ratio, add bacillus subtilis bacteria powder and the enzymatic microorganism of having dissolved, then above-mentioned substance is inserted to mixing corruption in pond and macerate, plastics film on upper cap, carry out fermenting in advance in 3-5 days; Then plastics film is thrown off, and stir, allow mixture contact oxygen, temperature gradually raises, from normal temperature to 45 ℃, keep continuing turning after 2-3 days, then be raised to 60 ℃ from 45 ℃, the hold-time is 3-5 days, then cool the temperature to normal temperature, keep 7-10 days, until slaking is fully fermented complete;
(3) solid photosynthetic bacterium, bacteriophagic Bdellovibrio are dissolved in water, join in said mixture, stir;
(4) by above-mentioned fermented product, air dry in the sun, make moisture content of material less than 25%, add kongpo monkshood root 45-55 part, Semen Momordicae 40-50 part, Stellera chamaejasme L. 1-15 part, hops 5-10 part, matrine 5-10 part, Golden Larch Bark 10-15 part, Chinese prickly ash 20-30 part, ginkgo kind skin 20-30 part, Fructus Cnidii 20-30 part, Cortex Meliae 10-30 part, Rangooncreeper Fruit 15-25 part, Turkey-galls 1-15 part, Root of Pubescent Holly 5-15 part, Seed of Chinese Soapberry exosper 55-60 part, fructus ulmi 10-15 part, then camphor 5-15 part pulverizes, pulverize fineness for passing through without screen pulverizer, after pulverizing, in material, add the mineral substance addition material, sequestrant and VITAMIN addition material, mix and obtain microbial fish guano.;
The using method of stichopus japonicus fertilizer is characterized in that: throw something and feed: in March in spring ~ May, should manually throw something and feed, do not throw something and feed July ~ September, and about mid-September, water temperature is fallen 18 ℃, enters stichopus japonicus and grow the thermophilic phase, strengthen daily ration, feeding quantity.Below 5 ℃, substantially do not ingest and stop throwing something and feeding.
Embodiment 2
The preparation method is with embodiment 1.
At the juvenile stage of stichopus japonicus growth, pull out, minute support in the middle of five ponds, 500, every pond, (contrast 1, originate from four ponds, according to the injected volume of every mu of 200kg, to throw in respectively the microbial fish guano of the embodiment of the present invention 1 and common fish guano Beijing Century AMMS Biotechnology Co., Ltd.), fish guano (contrast 2) is not thrown in the another one pond, but throws in the common fish food of same amount, and remaining feeding amount is identical, and culture environment is identical, through the cultivation of 2-4 month, contrasts the upgrowth situation of its output and stichopus japonicus, specifically as shown in Table 1 and Table 2:
Microbial fish guano prepared by table 1 the present invention and contrast 1 are relatively
Figure 2013103090927100002DEST_PATH_IMAGE002
Output increases (%) Individual increase (%) The fish reduction of dying of illness (%) EPA content increment (%)
Embodiment 1 22 15 14 13
Microbial fish guano prepared by table 2 the present invention and contrast 2 are relatively
Output increases (%) The fish body increases (%) The fish reduction of dying of illness (%) EPA content increment (%)
Embodiment 1 38 25 28 65
Contrast through table 1, table 2 can be found, adds the Fast Growth that composite microbe fertilizer of the present invention can promote stichopus japonicus, and EPA content increases, fish disease reduces, the fish that dies of illness reduces, and the beneficial microorganism in water body is increased, and final quality and quality all are improved.

Claims (6)

1. stichopus japonicus fertilizer, it is characterized in that, its raw material comprises the composition of following weight ratio: bacillus subtilis bacteria powder 2-4 part, enzymatic microorganism 1-4 part, solid photosynthetic bacterium 0.5-0.9 part, bacteriophagic Bdellovibrio 0.8-1.2 part, VITAMIN addition material 0.2-0.6 part, mineral substance addition material 10-16 part, sequestrant 0.2-0.4 part, Herba Eichhorniae 60-80 part, edible fungi residue 50-80 part, vinasse 100-110 part, fishbone powder 40-60 part, rice bran 70-80 part, brown sugar 10-16 part, kongpo monkshood root 45-55 part, Semen Momordicae 40-50 part, Stellera chamaejasme L. 1-15 part, hops 5-10 part, matrine 5-10 part, Golden Larch Bark 10-15 part, Chinese prickly ash 20-30 part, ginkgo kind skin 20-30 part, Fructus Cnidii 20-30 part, Cortex Meliae 10-30 part, Rangooncreeper Fruit 15-25 part, Turkey-galls 1-15 part, Root of Pubescent Holly 5-15 part, Seed of Chinese Soapberry exosper 55-60 part, fructus ulmi 10-15 part, camphor 5-15 part.
2. a kind of stichopus japonicus fertilizer according to claim 1, is characterized in that, described VITAMIN addition material component recipe ratio is: vitamin A, C, E mix with the weight ratio of 2:3:5.
3. a kind of stichopus japonicus fertilizer according to claim 1, it is characterized in that, described mineral substance addition material is potassium primary phosphate 20-25%, monocalcium phosphate 10-15%, ferrous sulfate 2-5%, zinc sulfate 1-2%, manganous sulfate 0.8-1%, borax 0.3-0.5%, rose vitriol 0.03-0.06%, rare earth 0.2-0.6%, and all the other add salt to 100%.
4. a kind of stichopus japonicus fertilizer according to claim 1, is characterized in that, to be sodium humate and EDTA mix with the weight ratio of 1:1-2 described sequestrant.
5. the preparation method of a kind of stichopus japonicus fertilizer according to claim 1, is characterized in that, comprises the following steps:
(1) at first Herba Eichhorniae, edible fungi residue, vinasse are dried in the sun to water content below 40%, then according to described ratio, add fishbone powder, rice bran, brown sugar, fully stir, mix;
(2) in said mixture, according to described ratio, add bacillus subtilis bacteria powder and the enzymatic microorganism of having dissolved, then above-mentioned substance is inserted to mixing corruption in pond and macerate, plastics film on upper cap, carry out fermenting in advance in 3-5 days; Then plastics film is thrown off, and stir, allow mixture contact oxygen, temperature gradually raises, from normal temperature to 45 ℃, keep continuing turning after 2-3 days, then be raised to 60 ℃ from 45 ℃, the hold-time is 3-5 days, then cool the temperature to normal temperature, keep 7-10 days, until slaking is fully fermented complete;
(3) solid photosynthetic bacterium, bacteriophagic Bdellovibrio are dissolved in water, join in said mixture, stir;
(4) by above-mentioned fermented product, air dry in the sun, make moisture content of material less than 25%, add kongpo monkshood root 45-55 part, Semen Momordicae 40-50 part, Stellera chamaejasme L. 1-15 part, hops 5-10 part, matrine 5-10 part, Golden Larch Bark 10-15 part, Chinese prickly ash 20-30 part, ginkgo kind skin 20-30 part, Fructus Cnidii 20-30 part, Cortex Meliae 10-30 part, Rangooncreeper Fruit 15-25 part, Turkey-galls 1-15 part, Root of Pubescent Holly 5-15 part, Seed of Chinese Soapberry exosper 55-60 part, fructus ulmi 10-15 part, then camphor 5-15 part pulverizes, pulverize fineness for passing through without screen pulverizer, after pulverizing, in material, add the mineral substance addition material, sequestrant and VITAMIN addition material, mix and obtain microbial fish guano.
6. the using method of stichopus japonicus fertilizer as claimed in claim 1, it is characterized in that: throw something and feed: in March in spring ~ May, should manually throw something and feed, do not throw something and feed July ~ September, and about mid-September, water temperature is fallen 18 ℃, enters stichopus japonicus and grows the thermophilic phase, strengthen daily ration, feeding quantity;
Below 5 ℃, substantially do not ingest and stop throwing something and feeding.
CN201310309092.7A 2013-07-23 2013-07-23 Stichopus japonicus fertilizer, and preparation and application methods thereof Expired - Fee Related CN103396210B (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104886360A (en) * 2015-04-27 2015-09-09 广西贵港东琥农业开发有限公司 Special fish fertilizer for aquaculture and preparation method of special fish fertilizer
CN105175153A (en) * 2015-10-14 2015-12-23 武汉瑞泽园生物环保科技有限公司 Bio-organic fertilizer produced by using edible fungus residues as main material and preparation method thereof
CN114223777A (en) * 2021-12-14 2022-03-25 辽宁省海洋水产科学研究院 Compound microecological preparation for inhibiting pathogenic vibrios for cultivating stichopus japonicus

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101225007A (en) * 2008-02-03 2008-07-23 武汉市科洋生物工程有限公司 Special biological fish-fertilizer for aquaculture and preparation method thereof
CN101305696A (en) * 2008-06-27 2008-11-19 张士华 Sandy pool Apostichopus japonicus ecological regulation and cultivation method
CN102972660A (en) * 2012-12-13 2013-03-20 刘宏生 Chinese herbal medicine compound bait for improving growing power, immunity and disease resistance of stichopus japonicus
CN103039727A (en) * 2012-12-12 2013-04-17 辽宁医学院 Preparation method of stichopus japonicus fermented feed capable of replacing alga
ES2406394A1 (en) * 2011-12-04 2013-06-06 Juan José FERRO ARCA Bait for nasa and longline (Machine-translation by Google Translate, not legally binding)

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101225007A (en) * 2008-02-03 2008-07-23 武汉市科洋生物工程有限公司 Special biological fish-fertilizer for aquaculture and preparation method thereof
CN101305696A (en) * 2008-06-27 2008-11-19 张士华 Sandy pool Apostichopus japonicus ecological regulation and cultivation method
ES2406394A1 (en) * 2011-12-04 2013-06-06 Juan José FERRO ARCA Bait for nasa and longline (Machine-translation by Google Translate, not legally binding)
CN103039727A (en) * 2012-12-12 2013-04-17 辽宁医学院 Preparation method of stichopus japonicus fermented feed capable of replacing alga
CN102972660A (en) * 2012-12-13 2013-03-20 刘宏生 Chinese herbal medicine compound bait for improving growing power, immunity and disease resistance of stichopus japonicus

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104886360A (en) * 2015-04-27 2015-09-09 广西贵港东琥农业开发有限公司 Special fish fertilizer for aquaculture and preparation method of special fish fertilizer
CN105175153A (en) * 2015-10-14 2015-12-23 武汉瑞泽园生物环保科技有限公司 Bio-organic fertilizer produced by using edible fungus residues as main material and preparation method thereof
CN114223777A (en) * 2021-12-14 2022-03-25 辽宁省海洋水产科学研究院 Compound microecological preparation for inhibiting pathogenic vibrios for cultivating stichopus japonicus
CN114223777B (en) * 2021-12-14 2023-07-07 辽宁省海洋水产科学研究院 Composite microecological preparation for inhibiting pathogenic vibrio for breeding stichopus japonicus

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