CN105767598A - Phagostimulant preparation method based on edible fungus cultivation waste - Google Patents
Phagostimulant preparation method based on edible fungus cultivation waste Download PDFInfo
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- CN105767598A CN105767598A CN201610173026.5A CN201610173026A CN105767598A CN 105767598 A CN105767598 A CN 105767598A CN 201610173026 A CN201610173026 A CN 201610173026A CN 105767598 A CN105767598 A CN 105767598A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
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Abstract
The present invention discloses a phagostimulant preparation method based on edible fungus cultivation waste and belongs to the technical field of waste utilization. Aiming at the problems that the current unified edible fungus cultivation waste treatment methods mainly adopt simple stacking and burning, which pollute environment and waste the biomass energy and useful substances which are rich in the edible fungus cultivation waste, and at the same time, the current phagostimulant is relatively single in efficacy, easy to produce drug residues in freshwater fish body, and obvious in toxic or side effects, the present invention provides the phagostimulant preparation method based on the edible fungus cultivation waste. Firstly, microbial promoting substances are used to promote the growth of the microorganisms in the edible fungus cultivation waste, then the obtained materials are mixed with soybean meal and nutrition soil, earthworms are used to assist decomposition, at the same time mucus produced by the earthworms is absorbed to attract fish to eat, then malic acid is added to adjust the flavor, red yeast rice powder is used to change colors and other substances are used to increase nutrients, and finally natural rubber is used to conduct wrapping to prepare the biodegradable phagostimulant which is free of toxic or side effects.
Description
Technical field
The invention discloses a kind of method preparing phagostimulant based on edible fungus bran, belong to waste utilization technical field.
Background technology
At present, Edible Fungi Industry Development is rapid, produces a large amount of mushroom bran.The process problem of a large amount of solid waste mushroom brans becomes one of key factor of affecting mushroom industry sustainable development.The method that world mushroom bran traditionally processes is mainly simple and easy bank up and burns, but due to the easy cooperating microorganisms of mushroom bran, not only becomes the main living contaminants source of periphery Edible Fungi, it is also possible to can cause potential bio-safety risk；The a large amount of mushroom brans produced arbitrarily are banked up, not only occupies a large amount of soil, and the major source of periphery water, soil, gas pollution can be become；It addition, the burning of mushroom bran both contaminated airs, waste again in mushroom bran rich in biomass energy and utility.Edible fungus bran, can be described as again mushroom bran, mushroom bran and mushroom bran etc., is with cotton seed hulls, wood flour, Caulis et Folium Oryzae, corn cob or multiple kinds of crops straw, industrial waste for primary raw material, solid medium discarded after producing edible fungi.After the zymolysis of the various enzymes that these composts are secreted through edible fungi mycelium, the macromolecular substances such as cellulose, hemicellulose, lignin, protein is all by degraded in various degree and utilization.And the content of crude protein and crude fat improves；And rich in several kinds of mineral elements such as aminoacid, polysaccharide and calcium, phosphorus, ferrum, zinc；Also create organic acid and bioactive substance etc. simultaneously, this adds increased the content of mushroom bran Middle nutrition composition.
Phagostimulant major part in the market is flavouring agent or sweeting agent, and effect is comparatively single, the olfactory sensation of fresh-water fishes or vision or the sense of taste etc. can only be formed single stimulation；And the constituent of some flavouring agent or sweeting agent comes from chemically synthesized product, in fresh-water fishes body, easily produce drug residue, toxic and side effects obvious.
Summary of the invention
nullThe technical problem that present invention mainly solves: the method processed for the upper mushroom bran of at present system is mainly simple and easy bank up and burns，Contaminated environment，Waste wherein rich in biomass energy and utility，Phagostimulant effect is comparatively single at present simultaneously，In fresh-water fishes body, easily produce drug residue、The obvious problem of toxic and side effects，The invention provides a kind of method preparing phagostimulant based on edible fungus bran，First with microorganism, the present invention promotes that material promotes microbial growth in edible fungus bran，Again it is mixed with bean cake and Nutrition Soil，Lumbricus is used to carry out assisting to decompose to it，Mucus produced by absorption Lumbricus simultaneously，Attract Fish feed，Then its taste is regulated again through interpolation malic acid，Hongqu powder (red colouring agent) changes color and other materials and has additional nutrients thing，Natural gum is finally used to wrap up，Have no side effect thus being prepared into，Biodegradable phagostimulant.
In order to solve above-mentioned technical problem, the technical solution adopted in the present invention is:
(1) weigh edible fungus bran to put in pulverizer and pulverize, cross 80 mesh sieves, again by solid-to-liquid ratio 1:2, the granule of gained after sieving is mixed homogeneously with the heteroauxing alcoholic solution that mass fraction is 10%, the ammonium bicarbonate soln using mass fraction to be 10% regulates pH to 7.5～8.0, at 30～35 DEG C, stands 1～2h after stirring 20～30min with 120r/min, it is filtered, collects filtrate；
(2) 5:2:1 in mass ratio, by the filtrate of above-mentioned gained, Nutrition Soil and bean cake mix homogeneously, it is added thereto to distilled water again, making its water content is 60～65%, is mixing uniformly to form cultivation matrix, is layed in culture pond by cultivation matrix subsequently, laying depth is 10～15cm, sealing culture pond, keeping its interior temperature is 35～40 DEG C, seals 2～3 days；
(3) after above-mentioned sealing terminates, above-mentioned cultivation matrix is carried out turning, after gravity-flow ventilation 10～12h, matrix 1000～1500 Lumbricus ovum of inoculation are cultivated by every square, being inoculated in by Lumbricus ovum in cultivation matrix, keeping the temperature in culture pond is 25～28 DEG C, and the water content cultivating matrix remains 60～65%, cultivate 12～16 days, subsequently the Lumbricus in culture medium body is taken out；
(4) count by weight, take the cultivation matrix after 60～70 parts of above-mentioned Lumbricuss are taken out, 4～6 parts of malic acids, 8～12 parts of Hongqu powder (red colouring agent)s, 15～20 parts of blood powders and 12～16 parts of Carnis Bovis seu Bubali creams, put into after stirring in fermentation tank, keeping temperature is 30～35 DEG C, setting speed is 150～180r/min, stirring fermentation 2～3 days, and every 7～8h is added thereto to wait the blood powder of number, after fermentation ends, remove fermentation liquid, collect fermented product；
(5) fermented product of above-mentioned gained is put into the sterilization that carries out disinfection in ultraviolet sterilizer, put it into subsequently in container, it is added thereto to the carrageenan solutions that mass fraction is 15%, when its viscosity reaches 300～360mPa s, stop adding carrageenan solutions, subsequently mixture is put in baking oven, it is dried overnight at 70 DEG C, again dried object is put in pulverizer and pulverize, cross 150 mesh sieves, phagostimulant can be obtained.
The application process of the present invention: the phagostimulant of gained of the present invention is mixed with fish foodstuff, the addition of phagostimulant is the 8～12% of fish foodstuff quality, rate 3～6% is raised subsequently by throwing, carry out feeding fresh-water fishes, every day feeding 3 times, it be observed that detect the feed intake of fresh-water fishes increases by 10～15%, growth cycle shortens 20～25%, survival rate is to 96～98%, and compared with the fresh-water fishes of the phagostimulant obtained by the non-feeding present invention, its quality increases by 9～14%.
Beneficial effects of the present invention:
(1) present invention makes full use of edible fungus bran, it is achieved that the utilization of resource, it is to avoid the pollution of environment；
(2) phagostimulant obtained by the present invention improves the speed of growth of feed intake and fish, shortens growth cycle, improves survival rate, biodegradable, has no side effect, and will not produce secondary pollution.
Detailed description of the invention
Weigh edible fungus bran to put in pulverizer and pulverize, cross 80 mesh sieves, again by solid-to-liquid ratio 1:2, the granule of gained after sieving is mixed homogeneously with the heteroauxing alcoholic solution that mass fraction is 10%, the ammonium bicarbonate soln using mass fraction to be 10% regulates pH to 7.5～8.0, at 30～35 DEG C, stands 1～2h after stirring 20～30min with 120r/min, it is filtered, collects filtrate；5:2:1 in mass ratio, by the filtrate of above-mentioned gained, Nutrition Soil and bean cake mix homogeneously, it is added thereto to distilled water again, making its water content is 60～65%, is mixing uniformly to form cultivation matrix, is layed in culture pond by cultivation matrix subsequently, laying depth is 10～15cm, sealing culture pond, keeping its interior temperature is 35～40 DEG C, seals 2～3 days；After above-mentioned sealing terminates, above-mentioned cultivation matrix is carried out turning, after gravity-flow ventilation 10～12h, matrix 1000～1500 Lumbricus ovum of inoculation are cultivated by every square, being inoculated in by Lumbricus ovum in cultivation matrix, keeping the temperature in culture pond is 25～28 DEG C, and the water content cultivating matrix remains 60～65%, cultivate 12～16 days, subsequently the Lumbricus in culture medium body is taken out；Count by weight, take the cultivation matrix after 60～70 parts of above-mentioned Lumbricuss are taken out, 4～6 parts of malic acids, 8～12 parts of Hongqu powder (red colouring agent)s, 15～20 parts of blood powders and 12～16 parts of Carnis Bovis seu Bubali creams, put into after stirring in fermentation tank, keeping temperature is 30～35 DEG C, setting speed is 150～180r/min, stirring fermentation 2～3 days, and every 7～8h is added thereto to wait the blood powder of number, after fermentation ends, remove fermentation liquid, collect fermented product；
The fermented product of above-mentioned gained is put into the sterilization that carries out disinfection in ultraviolet sterilizer, put it into subsequently in container, it is added thereto to the carrageenan solutions that mass fraction is 15%, when its viscosity reaches 300～360mPa s, stop adding carrageenan solutions, subsequently mixture is put in baking oven, it is dried overnight at 70 DEG C, again dried object is put in pulverizer and pulverize, cross 150 mesh sieves, phagostimulant can be obtained.
Example 1
Weigh edible fungus bran to put in pulverizer and pulverize, cross 80 mesh sieves, again by solid-to-liquid ratio 1:2, the granule of gained after sieving is mixed homogeneously with the heteroauxing alcoholic solution that mass fraction is 10%, the ammonium bicarbonate soln using mass fraction to be 10% regulates pH to 7.5, at 30 DEG C, stands 1h after stirring 20min with 120r/min, it is filtered, collects filtrate；5:2:1 in mass ratio, by the filtrate of above-mentioned gained, Nutrition Soil and bean cake mix homogeneously, it is added thereto to distilled water again, making its water content is 60%, is mixing uniformly to form cultivation matrix, is layed in culture pond by cultivation matrix subsequently, laying depth is 10cm, sealing culture pond, keeping its interior temperature is 35 DEG C, seals 2 days；After above-mentioned sealing terminates, above-mentioned cultivation matrix is carried out turning, after gravity-flow ventilation 10h, matrix 1000 Lumbricus ovum of inoculation are cultivated by every square, being inoculated in by Lumbricus ovum in cultivation matrix, keeping the temperature in culture pond is 25 DEG C, and the water content cultivating matrix remains 60%, cultivate 12 days, subsequently the Lumbricus in culture medium body is taken out；Count by weight, take the cultivation matrix after 60 parts of above-mentioned Lumbricuss are taken out, 6 parts of malic acids, 12 parts of Hongqu powder (red colouring agent)s, 20 parts of blood powders and 16 parts of Carnis Bovis seu Bubali creams, put into after stirring in fermentation tank, keeping temperature is 30 DEG C, setting speed is 150r/min, stirring fermentation 2 days, and every 7h is added thereto to wait the blood powder of number, after fermentation ends, remove fermentation liquid, collect fermented product；The fermented product of above-mentioned gained is put into the sterilization that carries out disinfection in ultraviolet sterilizer, put it into subsequently in container, it is added thereto to the carrageenan solutions that mass fraction is 15%, when its viscosity reaches 300mPa s, stop adding carrageenan solutions, subsequently mixture is put in baking oven, it is dried overnight at 70 DEG C, again dried object is put in pulverizer and pulverize, cross 150 mesh sieves, phagostimulant can be obtained.
The phagostimulant of gained of the present invention is mixed with fish foodstuff, the addition of phagostimulant is the 8% of fish foodstuff quality, rate 3% is raised subsequently by throwing, carry out feeding fresh-water fishes, every day feeding 3 times, it be observed that detect the feed intake of fresh-water fishes increases by 10%, growth cycle shortens 20%, survival rate is to 96%, and compared with the fresh-water fishes of the phagostimulant obtained by the non-feeding present invention, its quality increases by 9%.
Example 2
Weigh edible fungus bran to put in pulverizer and pulverize, cross 80 mesh sieves, again by solid-to-liquid ratio 1:2, the granule of gained after sieving is mixed homogeneously with the heteroauxing alcoholic solution that mass fraction is 10%, the ammonium bicarbonate soln using mass fraction to be 10% regulates pH to 8.0, at 35 DEG C, stands 2h after stirring 30min with 120r/min, it is filtered, collects filtrate；5:2:1 in mass ratio, by the filtrate of above-mentioned gained, Nutrition Soil and bean cake mix homogeneously, it is added thereto to distilled water again, making its water content is 65%, is mixing uniformly to form cultivation matrix, is layed in culture pond by cultivation matrix subsequently, laying depth is 15cm, sealing culture pond, keeping its interior temperature is 40 DEG C, seals 3 days；After above-mentioned sealing terminates, above-mentioned cultivation matrix is carried out turning, after gravity-flow ventilation 12h, matrix 1500 Lumbricus ovum of inoculation are cultivated by every square, being inoculated in by Lumbricus ovum in cultivation matrix, keeping the temperature in culture pond is 28 DEG C, and the water content cultivating matrix remains 65%, cultivate 16 days, subsequently the Lumbricus in culture medium body is taken out；Count by weight, take the cultivation matrix after 70 parts of above-mentioned Lumbricuss are taken out, 4 parts of malic acids, 8 parts of Hongqu powder (red colouring agent)s, 15 parts of blood powders and 12 parts of Carnis Bovis seu Bubali creams, put into after stirring in fermentation tank, keeping temperature is 35 DEG C, setting speed is 180r/min, stirring fermentation 3 days, and every 8h is added thereto to wait the blood powder of number, after fermentation ends, remove fermentation liquid, collect fermented product；The fermented product of above-mentioned gained is put into the sterilization that carries out disinfection in ultraviolet sterilizer, put it into subsequently in container, it is added thereto to the carrageenan solutions that mass fraction is 15%, when its viscosity reaches 360mPa s, stop adding carrageenan solutions, subsequently mixture is put in baking oven, it is dried overnight at 70 DEG C, again dried object is put in pulverizer and pulverize, cross 150 mesh sieves, phagostimulant can be obtained.
The phagostimulant of gained of the present invention is mixed with fish foodstuff, the addition of phagostimulant is the 12% of fish foodstuff quality, rate 6% is raised subsequently by throwing, carry out feeding fresh-water fishes, every day feeding 3 times, it be observed that detect the feed intake of fresh-water fishes increases by 15%, growth cycle shortens 25%, survival rate is to 98%, and compared with the fresh-water fishes of the phagostimulant obtained by the non-feeding present invention, its quality increases by 14%.
Example 3
Weigh edible fungus bran to put in pulverizer and pulverize, cross 80 mesh sieves, again by solid-to-liquid ratio 1:2, the granule of gained after sieving is mixed homogeneously with the heteroauxing alcoholic solution that mass fraction is 10%, the ammonium bicarbonate soln using mass fraction to be 10% regulates pH to 8.0, at 32 DEG C, stands 1.5h after stirring 25min with 120r/min, it is filtered, collects filtrate；5:2:1 in mass ratio, by the filtrate of above-mentioned gained, Nutrition Soil and bean cake mix homogeneously, it is added thereto to distilled water again, making its water content is 62%, is mixing uniformly to form cultivation matrix, is layed in culture pond by cultivation matrix subsequently, laying depth is 12cm, sealing culture pond, keeping its interior temperature is 38 DEG C, seals 3 days；After above-mentioned sealing terminates, above-mentioned cultivation matrix is carried out turning, after gravity-flow ventilation 11h, matrix 1300 Lumbricus ovum of inoculation are cultivated by every square, being inoculated in by Lumbricus ovum in cultivation matrix, keeping the temperature in culture pond is 26 DEG C, and the water content cultivating matrix remains 62%, cultivate 14 days, subsequently the Lumbricus in culture medium body is taken out；Count by weight, take the cultivation matrix after 68 parts of above-mentioned Lumbricuss are taken out, 5 parts of malic acids, 11 parts of Hongqu powder (red colouring agent)s, 18 parts of blood powders and 15 parts of Carnis Bovis seu Bubali creams, put into after stirring in fermentation tank, keeping temperature is 32 DEG C, setting speed is 170r/min, stirring fermentation 3 days, and every 7h is added thereto to wait the blood powder of number, after fermentation ends, remove fermentation liquid, collect fermented product；The fermented product of above-mentioned gained is put into the sterilization that carries out disinfection in ultraviolet sterilizer, put it into subsequently in container, it is added thereto to the carrageenan solutions that mass fraction is 15%, when its viscosity reaches 340mPa s, stop adding carrageenan solutions, subsequently mixture is put in baking oven, it is dried overnight at 70 DEG C, again dried object is put in pulverizer and pulverize, cross 150 mesh sieves, phagostimulant can be obtained.
The phagostimulant of gained of the present invention is mixed with fish foodstuff, the addition of phagostimulant is the 10% of fish foodstuff quality, rate 5% is raised subsequently by throwing, carry out feeding fresh-water fishes, every day feeding 3 times, it be observed that detect the feed intake of fresh-water fishes increases by 13%, growth cycle shortens 23%, survival rate is to 97%, and compared with the fresh-water fishes of the phagostimulant obtained by the non-feeding present invention, its quality increases by 12%.
Claims (1)
1. the method preparing phagostimulant based on edible fungus bran, it is characterised in that concrete preparation process is:
(1) weigh edible fungus bran to put in pulverizer and pulverize, cross 80 mesh sieves, again by solid-to-liquid ratio 1:2, the granule of gained after sieving is mixed homogeneously with the heteroauxing alcoholic solution that mass fraction is 10%, the ammonium bicarbonate soln using mass fraction to be 10% regulates pH to 7.5～8.0, at 30～35 DEG C, stands 1～2h after stirring 20～30min with 120r/min, it is filtered, collects filtrate；
(2) 5:2:1 in mass ratio, by the filtrate of above-mentioned gained, Nutrition Soil and bean cake mix homogeneously, it is added thereto to distilled water again, making its water content is 60～65%, is mixing uniformly to form cultivation matrix, is layed in culture pond by cultivation matrix subsequently, laying depth is 10～15cm, sealing culture pond, keeping its interior temperature is 35～40 DEG C, seals 2～3 days；
(3) after above-mentioned sealing terminates, above-mentioned cultivation matrix is carried out turning, after gravity-flow ventilation 10～12h, matrix 1000～1500 Lumbricus ovum of inoculation are cultivated by every square, being inoculated in by Lumbricus ovum in cultivation matrix, keeping the temperature in culture pond is 25～28 DEG C, and the water content cultivating matrix remains 60～65%, cultivate 12～16 days, subsequently the Lumbricus in culture medium body is taken out；
(4) count by weight, take the cultivation matrix after 60～70 parts of above-mentioned Lumbricuss are taken out, 4～6 parts of malic acids, 8～12 parts of Hongqu powder (red colouring agent)s, 15～20 parts of blood powders and 12～16 parts of Carnis Bovis seu Bubali creams, put into after stirring in fermentation tank, keeping temperature is 30～35 DEG C, setting speed is 150～180r/min, stirring fermentation 2～3 days, and every 7～8h is added thereto to wait the blood powder of number, after fermentation ends, remove fermentation liquid, collect fermented product；
(5) fermented product of above-mentioned gained is put into the sterilization that carries out disinfection in ultraviolet sterilizer, put it into subsequently in container, it is added thereto to the carrageenan solutions that mass fraction is 15%, when its viscosity reaches 300～360mPa s, stop adding carrageenan solutions, subsequently mixture is put in baking oven, it is dried overnight at 70 DEG C, again dried object is put in pulverizer and pulverize, cross 150 mesh sieves, phagostimulant can be obtained.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106490392A (en) * | 2016-10-11 | 2017-03-15 | 常州市鼎日环保科技有限公司 | A kind of method for preparing aquatic attractant with Lumbricuss silkworm silk |
| CN106721057A (en) * | 2016-12-09 | 2017-05-31 | 天津商业大学 | A kind of potent canine phagostimulant |
| CN106962693A (en) * | 2017-05-16 | 2017-07-21 | 烟台远景生物技术股份有限公司 | A kind of sea cucumber phagostimulant and preparation method thereof |
| CN108782490A (en) * | 2018-07-04 | 2018-11-13 | 烟台华知知识产权服务有限公司 | A kind of preparation method of earthworm bait |
-
2016
- 2016-03-24 CN CN201610173026.5A patent/CN105767598A/en not_active Withdrawn
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106490392A (en) * | 2016-10-11 | 2017-03-15 | 常州市鼎日环保科技有限公司 | A kind of method for preparing aquatic attractant with Lumbricuss silkworm silk |
| CN106721057A (en) * | 2016-12-09 | 2017-05-31 | 天津商业大学 | A kind of potent canine phagostimulant |
| CN106962693A (en) * | 2017-05-16 | 2017-07-21 | 烟台远景生物技术股份有限公司 | A kind of sea cucumber phagostimulant and preparation method thereof |
| CN108782490A (en) * | 2018-07-04 | 2018-11-13 | 烟台华知知识产权服务有限公司 | A kind of preparation method of earthworm bait |
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Application publication date: 20160720 |