CN106490392A - A kind of method for preparing aquatic attractant with Lumbricuss silkworm silk - Google Patents

Abstract

The present invention relates to a kind of method for preparing aquatic attractant with Lumbricuss silkworm silk, belongs to feed additive preparing technical field.The present invention first Lumbricuss are crushed after with wheat bran, peanut cake powder co-fermentation, obtain fermentation liquid, containing several amino acids and Attractant in fermentation liquid, and the mucus in Lumbricuss body can stimulate the olfactory sensation of aquatic products animal, induction aquatic animal feeding, contain abundant aminoacid and nucleotide in Bombyx bombycis and Sanguis Gallus domesticus, contain glycine betaine in Radix Betae, and in mixing anaerobic is mouldy, the probioticss such as lactic acid bacteria can be grown, and secrete the materials such as lactic acid, aquatic animal dyspeptic phenomenon can also be improved while the aquatic animal sense of taste is stimulated, promote the absorption of feedstuff, neohesperidin dihydrochalcone has sweet taste, and sweet taste is clearly happy, the bitterness that may carry in aquatic animal feed can be effectively reduced.

Description

A kind of method for preparing aquatic attractant with Lumbricuss silkworm silk

Technical field

The present invention relates to a kind of method for preparing aquatic attractant with Lumbricuss silkworm silk, belongs to feed additive technology of preparing neck Domain.

Background technology

In recent years, as the shortage of fish flour resource, fish meal prices are made friends with all the way, Feed Enterprise is had to using improving feedstuff The consumption of middle vegetable protein is substituting the consumption of part or all of fish flour to reduce feed cost, but so has led to new The appearance of problem, as vegetable protein is in a large number using so as to have impact on local flavor, the adaptability of feedstuff, further, since plant egg Have that antinutritional factor, digestibility are low in vain, the speed of growth and gastrointestinal health for causing aquatic animal is subject to bad shadow Ring.

Phagostimulant is mainly by with necessarily volatile natural materials（The extractions such as root, stem, leaf, flower, fruit such as from plant Concentrated solution）With synthetic fragrance raw material（The such as compound such as aldehyde, ketone, alcohol, acid, ester, ether）It is formulated.Phagostimulant is a kind of The unified coalition of color.The characteristics of phagostimulant is：There is gay colours typically, vision can be stimulated, cause appetite；Dissipate The fragrance for sending out strong, infects the environment of surrounding, is searched for food by stimulating olfactory sensation, induced animal；There is good palatability, can stimulate The sense of taste, promotes feed intake.Color synergism, constitutes the basic feature of feed eating belt agent.

Aquatic attractant is also known as palatability additive, belongs to non-nutritive additive, and it includes promoting to take the photograph with stimulating animal Food various benefit materials, they there is obvious and positive biological action to aquatic animal, can effectively improve water The feed efficiency of animal is produced, reduces feeding cost.As aquatic animal vision is bad, and there is sensitive olfactory sensation, the sense of taste, raising Add in material phagostimulant not only can promote to look for food, improve feedstuff palatability, improve Feeding Velocity and food ration, promote life Long, and promote aquatic animal to digest and assimilate feedstuff, improve food conversion ratio, and then improve quality of the fodder performance comprehensively And culture benefit.

Aquatic attractant phagostimulating effect in the market is not good, it is impossible to improve digesting and assimilating for feedstuff, can cause Aquatic product Animal digestion is bad, though affect to produce the health of animal and growing state, additionally, existing phagostimulant addition in use Greatly, palatability is poor, and residual bait sinks under water after corruption, can also cause water pollution, affects growth of animal environment.

Content of the invention

The technical problem to be solved：Add for current aquatic attractant phagostimulating effect is good, during use Dosage is excessive, palatability is poor, it is impossible to improve the problem digested and assimilated of the aquatic animal to feedstuff, there is provided one kind uses Lumbricuss silkworm Silk prepare aquatic attractant method, the present invention first Lumbricuss are crushed after with wheat bran, peanut cake powder co-fermentation, obtain Fermentation liquid, can stimulate aquatic products to move containing the mucus in several amino acids and Attractant, and Lumbricuss body in fermentation liquid The olfactory sensation of thing, induction aquatic animal feeding, contains abundant aminoacid and nucleotide in Bombyx bombycis and Sanguis Gallus domesticus, contains Radix Betae in Radix Betae Alkali, and in mixing anaerobic is mouldy, the probioticss such as lactic acid bacteria can be grown, and secrete the materials such as lactic acid, stimulating aquatic animal Aquatic animal dyspeptic phenomenon can also be improved while the sense of taste, promote the absorption of feedstuff, neohesperidin dihydrochalcone to have Pleasantly sweet, and sweet taste is clearly happy, can be effectively reduced the bitterness that may carry in aquatic animal feed, and prepared by the present invention Substantially, addition is little in use, good palatability, can significantly improve aquatic animal for aquatic animal phagostimulant phagostimulating effect Feedstuff is digested and assimilated, is suitable for large-scale production and is used.

For solving above-mentioned technical problem, the technical solution used in the present invention is：

（1）Take 30～40g Lumbricuss and be put into rubbing in meat grinder, collect the Lumbricuss after rubbing, and add Lumbricuss quality 10～15% Wheat bran, the peanut cake powder of Lumbricuss amount 3～5% and 300～400mL deionized waters, after being uniformly mixed, are placed in sterilization tank, Sterilize at 110～120 DEG C 10～20min, is cooled to after room temperature with tank after which, moves in fermentation tank, subsequently connects by 8～10% The amount of kind is inoculated into aspergillus oryzae in fermentation tank, aerobic fermentation 3～4 days at 35～45 DEG C；Fermentation ends, are warming up to 100～110 DEG C, 5～10min is stirred, fermentation liquid is subsequently collected, standby；

（2）50～60g Bombyx bombycis are weighed, is washed with deionized 3～5 times, is subsequently placed in beaker, and add 300～400mL matter Amount fraction is 2% sodium carbonate liquor, is heated to seething with excitement, and is incubated 2～3h, subsequently filters, collects filtering residue, and be washed with deionized Filtering residue 3～5 times, then 1～2h of natural air drying, obtain dry silk element；

（3）30～40g Radix Betaes are weighed, and is squeezed the juice with juice extractor after being washed with deionized totally, collected beet juice, add 20 The above-mentioned dry plain silks of～30g, 50～60g Sanguis Gallus domesticus, after being uniformly mixed, obtain mixture, mixture are put in circular bottom pot, to 1～2L water is added wherein, pot cover is covered, is warming up to 95～100 DEG C, be incubated 30～40min of steaming and decocting, after the completion of steaming and decocting, be transferred to In tub, and with transparent plastic film sealing preserve 10～12 days so as to naturally mouldy；

（4）Subsequently plastic sheeting is removed, and will be mouldy after mixture be transferred to darkness, moist place's preservation 6～8 days so as to Naturally mouldy, after fungus growth to 5～8cm, 300～400mL deionized waters are added to, after stirring mixing, are filtered, is received Filtrate is carried out being heated to seething with excitement by collection filtrate, is incubated 10～20min, is subsequently naturally cooled to room temperature, be placed in centrifuge, 30～40min is centrifuged under 3000～4000r/min, supernatant is collected；

（5）By supernatant and step（1）After standby fermentation liquid mixing, 2～4g neohesperidin dihydrochalcones are added, is stirred After 10～20min, it is placed in freezer dryer, at -40～-30 DEG C, 24～36h of lyophilization, obtains dried object, by drying Thing is put in pulverizer and is crushed, and crosses 100～200 mesh sieves, collects the granule that sieves, you can obtain aquatic attractant.

The application process of the present invention：Aquatic attractant prepared by the present invention is added to feeding by 0.1～0.3% addition In material, subsequently aquatic animal is carried out feeding and being thrown, daily feeding is thrown 3～4 times.To detect, add the feedstuff of phagostimulant of the present invention, Infiltration rate of the aquatic animal to nutrient substance can be dramatically speeded up, is continuously fed 1～3 month, improve animal weightening 15～25%, Feedstuff palatability is improved, grazing speed 20～30% is effectively improved, residual bait amount reduces 40～60%.

Compared with additive method, Advantageous Effects are the present invention：

（1）Substantially, addition is little in use, good palatability for aquatic animal phagostimulant phagostimulating effect prepared by the present invention, Aquatic animal can be significantly improved to digest and assimilate feedstuff, be suitable for large-scale production and used；

（2）The main preparation process of the present invention is simple, required low cost.

Specific embodiment

Take 30～40g Lumbricuss first and be put in meat grinder and rub, collect the Lumbricuss after rubbing, and add Lumbricuss quality 10～ 15% wheat bran, the peanut cake powder of Lumbricuss amount 3～5% and 300～400mL deionized waters, after being uniformly mixed, are placed in sterilization tank In, sterilize at 110～120 DEG C 10～20min, is cooled to after room temperature with tank after which, moves in fermentation tank, subsequently presses 8～10% Inoculum concentration aspergillus oryzae is inoculated in fermentation tank, aerobic fermentation 3～4 days at 35～45 DEG C；Fermentation ends, are warming up to 100 ～110 DEG C, 5～10min is stirred, subsequently collect fermentation liquid, standby；Then 50～60g Bombyx bombycis are weighed, 3 are washed with deionized ～5 times, it is subsequently placed in beaker, and adds 300～400mL mass fractions for 2% sodium carbonate liquor, is heated to seething with excitement, insulation 2 ～3h, subsequently filters, and collects filtering residue, and is washed with deionized filtering residue 3～5 times, then 1～2h of natural air drying, obtains dry silk element；Claim 30～40g Radix Betaes are taken, and is squeezed the juice with juice extractor after being washed with deionized totally, collected beet juice, add 20～30g above-mentioned Dry element silk, 50～60g Sanguis Gallus domesticus, after being uniformly mixed, obtain mixture, mixture are put in circular bottom pot, 1 is added thereto to ～2L water, covers pot cover, is warming up to 95～100 DEG C, is incubated 30～40min of steaming and decocting, after the completion of steaming and decocting, is transferred in tub, and With transparent plastic film sealing preserve 10～12 days so as to naturally mouldy；Subsequently plastic sheeting is removed, and will be mouldy after mixed Compound is transferred at darkness, humidity and preserves 6～8 days so as to naturally mouldy, after fungus growth to 5～8cm, is added to 300～400mL deionized waters, stirring mixing after, filter, collect filtrate, carry out being heated to seething with excitement by filtrate, insulation 10～ 20min, subsequently naturally cools to room temperature, is placed in centrifuge, under 3000～4000r/min is centrifuged 30～40min, collects Supernatant；After supernatant is mixed with standby fermentation liquid, 2～4g neohesperidin dihydrochalcones are added, stirring 10～ After 20min, it is placed in freezer dryer, 24～36h of lyophilization at -40～-30 DEG C obtains dried object, dried object is put Enter in pulverizer and crushed, cross 100～200 mesh sieves, collect the granule that sieves, you can obtain aquatic attractant.

Example 1

Take 30g Lumbricuss first and be put into rubbing in meat grinder, collect the Lumbricuss after rubbing, and add the wheat bran of Lumbricuss quality 10%, earthworm The peanut cake powder of earthworm amount 3% and 300mL deionized waters, after being uniformly mixed, are placed in sterilization tank, sterilize at 110 DEG C 10min, is cooled to after room temperature with tank after which, moves in fermentation tank, aspergillus oryzae is inoculated into fermentation tank by 8% inoculum concentration subsequently In, aerobic fermentation 3 days at 35 DEG C；Fermentation ends, are warming up to 100 DEG C, stir 5min, subsequently collect fermentation liquid, standby；Then 50g Bombyx bombycis are weighed, is washed with deionized 3 times, is subsequently placed in beaker, and add 300mL mass fractions molten for 2% sodium carbonate Liquid, is heated to seething with excitement, and is incubated 2h, subsequently filters, and collects filtering residue, and is washed with deionized filtering residue 3 times, then natural air drying 1h, Obtain dry silk element；30g Radix Betaes are weighed, and is squeezed the juice with juice extractor after being washed with deionized totally, collected beet juice, add 20g Above-mentioned dry plain silk, 50g Sanguis Gallus domesticus, after being uniformly mixed, obtain mixture, mixture are put in circular bottom pot, are added thereto to 1L water, covers pot cover, is warming up to 95 DEG C, is incubated steaming and decocting 30min, after the completion of steaming and decocting, is transferred in tub, and thin with transparent plastic Film phonograph seal is preserved 10 days so as to naturally mouldy；Subsequently plastic sheeting is removed, and will be mouldy after mixture be transferred to darkness, Preserve 6 days at humidity so as to naturally mouldy, after fungus growth to 5cm, be added to 300mL deionized waters, stirring mixing Afterwards, filter, collect filtrate, carry out being heated to seething with excitement by filtrate, be incubated 10min, subsequently naturally cool to room temperature, be placed in being centrifuged In machine, under 3000r/min, 30min is centrifuged, collects supernatant；After supernatant is mixed with standby fermentation liquid, 2g is added Neohesperidin dihydrochalcone, after stirring 10min, is placed in freezer dryer, and lyophilization 24h at -40 DEG C obtains drying Thing, dried object is put in pulverizer and is crushed, and crosses 100 mesh sieves, collects the granule that sieves, you can obtain aquatic attractant.

Aquatic attractant prepared by the present invention is added in feedstuff by 0.1% addition, subsequently aquatic animal is carried out Feed and throw, daily feeding is thrown 3 times.To detect, and add the feedstuff of phagostimulant of the present invention, aquatic animal can be dramatically speeded up to nutrient The infiltration rate of matter, continuously feeds 1 month, improves animal weightening 15%, improves feedstuff palatability, effectively improve grazing speed 20%, residual bait amount reduces 40%.

Example 2

Take 40g Lumbricuss first and be put into rubbing in meat grinder, collect the Lumbricuss after rubbing, and add the wheat bran of Lumbricuss quality 15%, earthworm The peanut cake powder of earthworm amount 5% and 400mL deionized waters, after being uniformly mixed, are placed in sterilization tank, sterilize at 120 DEG C 20min, is cooled to after room temperature with tank after which, moves in fermentation tank, aspergillus oryzae is inoculated into fermentation tank by 10% inoculum concentration subsequently In, aerobic fermentation 4 days at 45 DEG C；Fermentation ends, are warming up to 110 DEG C, stir 10min, subsequently collect fermentation liquid, standby；So After weigh 60g Bombyx bombycis, be washed with deionized 5 times, be subsequently placed in beaker, and add 400mL mass fractions be 2% sodium carbonate Solution, is heated to seething with excitement, and is incubated 3h, subsequently filters, and collects filtering residue, and is washed with deionized filtering residue 5 times, then natural air drying 2h, obtains dry silk element；40g Radix Betaes are weighed, and is squeezed the juice with juice extractor after being washed with deionized totally, collected beet juice, add The above-mentioned dry plain silks of 30g, 60g Sanguis Gallus domesticus, after being uniformly mixed, obtain mixture, mixture are put in circular bottom pot, thereto plus Enter 2L water, cover pot cover, be warming up to 100 DEG C, be incubated steaming and decocting 40min, after the completion of steaming and decocting, be transferred in tub, and use transparent modeling Material diaphragm seal is preserved 12 days so as to naturally mouldy；Subsequently plastic sheeting is removed, and will be mouldy after mixture be transferred to the moon Secretly, preserve 8 days at humidity so as to naturally mouldy, after fungus growth to 8cm, be added to 400mL deionized waters, stir After mixing, filter, collect filtrate, carry out being heated to seething with excitement by filtrate, be incubated 20min, subsequently naturally cool to room temperature, be placed in In centrifuge, under 4000r/min, 40min is centrifuged, collects supernatant；After supernatant is mixed with standby fermentation liquid, then plus Enter 4g neohesperidin dihydrochalcones, after stirring 20min, be placed in freezer dryer, lyophilization 36h at -30 DEG C is obtained Dried object, dried object is put in pulverizer and is crushed, and crosses 200 mesh sieves, collects the granule that sieves, you can obtain Aquatic product food calling Agent.

Aquatic attractant prepared by the present invention is added in feedstuff by 0.2% addition, subsequently aquatic animal is carried out Feed and throw, daily feeding is thrown 3 times.To detect, and add the feedstuff of phagostimulant of the present invention, aquatic animal can be dramatically speeded up to nutrient The infiltration rate of matter, continuously feeds 2 months, improves animal weightening 20%, improves feedstuff palatability, effectively improve grazing speed 25%, residual bait amount reduces 50%.

Example 3

Take 35g Lumbricuss first and be put into rubbing in meat grinder, collect the Lumbricuss after rubbing, and add the wheat bran of Lumbricuss quality 13%, earthworm The peanut cake powder of earthworm amount 4% and 350mL deionized waters, after being uniformly mixed, are placed in sterilization tank, sterilize at 115 DEG C 15min, is cooled to after room temperature with tank after which, moves in fermentation tank, aspergillus oryzae is inoculated into fermentation tank by 9% inoculum concentration subsequently In, aerobic fermentation 3.5 days at 40 DEG C；Fermentation ends, are warming up to 105 DEG C, stir 8min, subsequently collect fermentation liquid, standby；So After weigh 55g Bombyx bombycis, be washed with deionized 4 times, be subsequently placed in beaker, and add 350mL mass fractions be 2% sodium carbonate Solution, is heated to seething with excitement, and is incubated 2.5h, subsequently filters, and collects filtering residue, and is washed with deionized filtering residue 4 times, then natural air drying 1.5h, obtains dry silk element；35g Radix Betaes are weighed, and is squeezed the juice with juice extractor after being washed with deionized totally, collection beet juice, then plus Enter the above-mentioned dry plain silks of 25g, 55g Sanguis Gallus domesticus, after being uniformly mixed, obtain mixture, mixture is put in circular bottom pot, thereto 1.5L water is added, pot cover is covered, is warming up to 98 DEG C, be incubated steaming and decocting 35min, after the completion of steaming and decocting, be transferred in tub, and with transparent Plastic sheeting sealing preserve 11 days so as to naturally mouldy；Subsequently plastic sheeting is removed, and will be mouldy after mixture be transferred to Preserve 7 days at dark, humidity so as to naturally mouldy, after fungus growth to 6cm, be added to 350mL deionized waters, stir After mixing mixing, filter, collect filtrate, carry out being heated to seething with excitement by filtrate, be incubated 15min, subsequently naturally cool to room temperature, juxtaposition In centrifuge, under 3500r/min, 35min is centrifuged, collects supernatant；After supernatant is mixed with standby fermentation liquid, then 3g neohesperidin dihydrochalcones are added, after stirring 15min, is placed in freezer dryer, lyophilization 30h at -35 DEG C is obtained To dried object, dried object is put in pulverizer and is crushed, cross 150 mesh sieves, collect the granule that sieves, you can obtain Aquatic product food calling Agent.

Aquatic attractant prepared by the present invention is added in feedstuff by 0.3% addition, subsequently aquatic animal is carried out Feed and throw, daily feeding is thrown 4 times.To detect, and add the feedstuff of phagostimulant of the present invention, aquatic animal can be dramatically speeded up to nutrient The infiltration rate of matter, continuously feeds 3 months, improves animal weightening 25%, improves feedstuff palatability, effectively improve grazing speed 30%, residual bait amount reduces 60%.

Claims (1)

1. a kind of method for preparing aquatic attractant with Lumbricuss silkworm silk, it is characterised in that concrete preparation process is：

（1）Take 30～40g Lumbricuss and be put into rubbing in meat grinder, collect the Lumbricuss after rubbing, and add Lumbricuss quality 10～15% Wheat bran, the peanut cake powder of Lumbricuss quality 3～5% and 300～400mL deionized waters, after being uniformly mixed, are placed in sterilization tank, Sterilize at 110～120 DEG C 10～20min, is cooled to after room temperature with tank after which, moves in fermentation tank, subsequently by 8～10% Inoculum concentration is inoculated into aspergillus oryzae in fermentation tank, aerobic fermentation 3～4 days at 35～45 DEG C；Fermentation ends, it is warming up to 100～ 110 DEG C, 5～10min is stirred, subsequently collect fermentation liquid, standby；

（2）50～60g Bombyx bombycis are weighed, is washed with deionized 3～5 times, is subsequently placed in beaker, and add 300～400mL matter Amount fraction is 2% sodium carbonate liquor, is heated to seething with excitement, and is incubated 2～3h, subsequently filters, collects filtering residue, and be washed with deionized Filtering residue 3～5 times, then 1～2h of natural air drying, obtain dry silk element；

（3）30～40g Radix Betaes are weighed, and is squeezed the juice with juice extractor after being washed with deionized totally, collected beet juice, add 20 The above-mentioned dry plain silks of～30g, 50～60g Sanguis Gallus domesticus, after being uniformly mixed, obtain mixture, mixture are put in circular bottom pot, to 1～2L water is added wherein, pot cover is covered, is warming up to 95～100 DEG C, be incubated 30～40min of steaming and decocting, after the completion of steaming and decocting, be transferred to In tub, and with transparent plastic film sealing preserve 10～12 days so as to naturally mouldy；

（4）Subsequently plastic sheeting is removed, and will be mouldy after mixture be transferred to darkness, moist place's preservation 6～8 days so as to Naturally mouldy, after fungus growth to 5～8cm, 300～400mL deionized waters are added to, after stirring mixing, are filtered, is received Filtrate is carried out being heated to seething with excitement by collection filtrate, is incubated 10～20min, is subsequently naturally cooled to room temperature, be placed in centrifuge, 30～40min is centrifuged under 3000～4000r/min, supernatant is collected；

（5）By supernatant and step（1）After standby fermentation liquid mixing, 2～4g neohesperidin dihydrochalcones are added, is stirred After 10～20min, it is placed in freezer dryer, at -40～-30 DEG C, 24～36h of lyophilization, obtains dried object, by drying Thing is put in pulverizer and is crushed, and crosses 100～200 mesh sieves, collects the granule that sieves, you can obtain aquatic attractant.