CN1879814B - Antivirus medicine for birds and method for preparing same - Google Patents
Antivirus medicine for birds and method for preparing same Download PDFInfo
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- CN1879814B CN1879814B CN2006100137156A CN200610013715A CN1879814B CN 1879814 B CN1879814 B CN 1879814B CN 2006100137156 A CN2006100137156 A CN 2006100137156A CN 200610013715 A CN200610013715 A CN 200610013715A CN 1879814 B CN1879814 B CN 1879814B
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Abstract
The invention discloses a fowl use antiviral medicament and process for preparation, which comprises the following raw materials (by weight portions): green chiretta 18-40 parts, radix isatidis 8-20 parts, cordate houttuynia 7-15 parts, capsule of weeping forsythia 3-12 parts, acorus gramineus soland 1-8 parts, wrinkled giant hyssop 1-8 parts, secretio bufonis 0.5-3 parts, borneol 3-8 parts, reed rhizome 3-9 parts, calcium sulphate 2-7 parts. The preparing process consists of steps of disintegrating, sieving, weighing and mixing homogeneously.
Description
Technical field
The present invention relates to stud bird medication thing and preparation method thereof, particularly a kind of antivirus medicine for birds and preparation method thereof.
Background technology
At present, aspect treating and preventing brutish disease, adopt chemical synthetic drug more,, have the halfway drawback of treatment though little, the instant effect of chemical synthetic drug dosage is cured the symptoms, not the disease sometimes; Particularly antibiotic use makes bacterial strain produce drug resistance easily.For viral infection, the effect of chemical synthetic drug does not all have reliable test and data, and the antiviral Western medicine cancelled use in brutish medicine in the end of the year 2005, makes the control of livestock and poultry virus disease face the awkward condition that no medicine can be executed.And viral infection usually takes place on animal body, in case occur, can cause heavy losses to aquaculture, also can cause potential harm to the mankind.For example: it is a kind of acute, the deadly infectious disease of chicken that newcastle disease virus infects, and is popular in all over the world, and particularly popular more extensive in Asia and China various places, the pathogen of newcastle disease is a kind of filterable virus, and the four seasons all can take place; Especially spring and autumn is more, and after chicken was subjected to extraneous viral infection, its symptom was: fervescence, and subtract suddenly and eat or do not eat, down in spirits, general weakness, cephalledema, necking down, crop inflation, feces are yellow green.This disease still not being had specific medicament both at home and abroad now can treat, and have only injection newcastle disease vaccine to prevent, but vaccine needs the low temperature keeping, is difficult to spread to vast rural area, and therefore, viral disease remains the formidable enemy of aquaculture.
Summary of the invention
The objective of the invention is to overcome above-mentioned weak point, provide a kind of and heighten the effect of a treatment, reduce cost and can effectively prevent and treat antivirus medicine for birds of the common viral disease of birds, upper respiratory tract infection and anemopyretic cold and preparation method thereof.
Implementation of the present invention is as follows for achieving the above object:
A kind of antivirus medicine for birds is characterized in that being formed by following portions by weight proportion raw material mixed preparing: Herba Andrographis 18-40 part, Radix Isatidis 8-20 part, Herba Houttuyniae 7-15 part, Fructus Forsythiae 3-12 part, Rhizoma Acori Graminei 1-8 part, Herba Pogostemonis 1-8 part, Venenum Bufonis 0.5-3 part, Borneolum Syntheticum 3-8 part, Rhizoma Phragmitis 3-9 part, Gypsum Fibrosum 2-7 part.
A kind of preparation method of antivirus medicine for birds is characterized in that carrying out as follows:
With following various raw materials: cross the 20-80 mesh sieve behind Herba Andrographis, Radix Isatidis, Herba Houttuyniae, Fructus Forsythiae, Rhizoma Acori Graminei, Herba Pogostemonis, Venenum Bufonis, Borneolum Syntheticum, Rhizoma Phragmitis, the Gypsum Fibrosum pulverize separately; Take by weighing Herba Andrographis 18-40 part by weight, Radix Isatidis 8-20 part, Herba Houttuyniae 7-15 part, Fructus Forsythiae 3-12 part, Rhizoma Acori Graminei 1-8 part, Herba Pogostemonis 1-8 part, Venenum Bufonis 0.5-3 part, Borneolum Syntheticum 3-8 part, Rhizoma Phragmitis 3-9 part, Gypsum Fibrosum 2-7 part; Uniform mixing.
The invention has the beneficial effects as follows: medicine of the present invention is that pure Chinese herbal medicine is formed, reasonable recipe, Radix Isatidis wherein, the bird flu H of the effective ingredient in the Fructus Forsythiae to just spreading in worldwide at present
5N
1And H
9N
2Subtype virus has good killing rate, cooperates Herba Andrographis, Herba Houttuyniae, Venenum Bufonis, and the energy better prevention comprises multiple viral disease, upper respiratory tract infection and the anemopyretic cold of bird flu.Medicine of the present invention is the principles of formulating prescriptions according to the diagnosis and treatment method of motherland's traditional Chinese medical science to " comprehensive adjustment, the mass prevention and mass treatment " of pestilence, heat, typhoid uniqueness in line with " heat-clearing and toxic substances removing, relieving the exterior syndrome with drugs of pungent in flavor and cool in nature should promote the production of body fluid by lung spleen reinforcing dampness "; Be equipped with Herba Andrographis, Radix Isatidis, suppress virus multiplication, have antiviral one's best quality.Maximum function promptly directly acts on the process that viral DNA or RNA (bird flu promptly belongs to this viroid) transcribe, and suppresses the breeding of virus, and the medicines of respectively distinguishing the flavor of such as Venenum Bufonis, Herba Houttuyniae have good heat clearing away, antidotal function simultaneously.Clinical trial shows that medicine of the present invention can reach more than 90% the control rate of viral disease, wherein to the effective percentage of control fowl anemopyretic cold, upper respiratory tract infection more than 94%, have unique curative effect.Drug toxicity of the present invention is little, and cost is lower, and is easy to use, can directly be added in the feedstuff, plays the preventive effect to virus.
The specific embodiment
The present invention is further illustrated below in conjunction with specific embodiment.
Embodiment 1
A kind of antivirus medicine for birds is characterized in that forming when 10g (every part for) by following portions by weight proportion raw material mixed preparing: Herba Andrographis 280g, Radix Isatidis 180g, Herba Houttuyniae 100g, Fructus Forsythiae 70g, Rhizoma Acori Graminei 50g, Herba Pogostemonis 70g, Venenum Bufonis 20g, Borneolum Syntheticum 50g, Rhizoma Phragmitis 70g, Gypsum Fibrosum 50g.
A kind of preparation method of antivirus medicine for birds is characterized in that carrying out as follows:
With following various raw materials: cross the 20-80 mesh sieve behind Herba Andrographis, Radix Isatidis, Herba Houttuyniae, Fructus Forsythiae, Rhizoma Acori Graminei, Herba Pogostemonis, Venenum Bufonis, Borneolum Syntheticum, Rhizoma Phragmitis, the Gypsum Fibrosum pulverize separately; Take by weighing Herba Andrographis 280g, Radix Isatidis 180g, Herba Houttuyniae 100g, Fructus Forsythiae 70g, Rhizoma Acori Graminei 50g, Herba Pogostemonis 70g, Venenum Bufonis 20g, Borneolum Syntheticum 50g, Rhizoma Phragmitis 70g, Gypsum Fibrosum 50g by weight; Uniform mixing, or mix homogeneously step by step, effect is better.
Embodiment 2
A kind of antivirus medicine for birds is characterized in that being formed by following portions by weight proportion raw material mixed preparing: Herba Andrographis 320g, Radix Isatidis 150g, Herba Houttuyniae 120g, Fructus Forsythiae 80g, Rhizoma Acori Graminei 70g, Herba Pogostemonis 50g, Venenum Bufonis 20g, Borneolum Syntheticum 80g, Rhizoma Phragmitis 50g, Gypsum Fibrosum 40g.
With following various raw materials: cross the 20-80 mesh sieve behind Herba Andrographis, Radix Isatidis, Herba Houttuyniae, Fructus Forsythiae, Rhizoma Acori Graminei, Herba Pogostemonis, Venenum Bufonis, Borneolum Syntheticum, Rhizoma Phragmitis, the Gypsum Fibrosum pulverize separately; Take by weighing Herba Andrographis 320g by weight, Radix Isatidis 150g, Herba Houttuyniae 120g, Fructus Forsythiae 80g, Rhizoma Acori Graminei 70g, Herba Pogostemonis 50g, Venenum Bufonis 20g, Borneolum Syntheticum 80g, Rhizoma Phragmitis 50g, Gypsum Fibrosum 40g; Uniform mixing.Other is with embodiment 1.
Embodiment 3
A kind of antivirus medicine for birds is characterized in that being formed by following portions by weight proportion raw material mixed preparing: Herba Andrographis 400g, Radix Isatidis 190g, Herba Houttuyniae 80g, Fructus Forsythiae 70g, Rhizoma Acori Graminei 60g, Herba Pogostemonis 40g, Venenum Bufonis 10g, Borneolum Syntheticum 60g, Rhizoma Phragmitis 50g, Gypsum Fibrosum 40g.
With following various raw materials: cross the 20-80 mesh sieve behind Herba Andrographis, Radix Isatidis, Herba Houttuyniae, Fructus Forsythiae, Rhizoma Acori Graminei, Herba Pogostemonis, Venenum Bufonis, Borneolum Syntheticum, Rhizoma Phragmitis, the Gypsum Fibrosum pulverize separately; Take by weighing Herba Andrographis 400g by weight, Radix Isatidis 190g, Herba Houttuyniae 80g, Fructus Forsythiae 70g, Rhizoma Acori Graminei 60g, Herba Pogostemonis 40g, Venenum Bufonis 10g, Borneolum Syntheticum 60g, Rhizoma Phragmitis 50g, Gypsum Fibrosum 40g; Uniform mixing.Other is with embodiment 1.
Embodiment 4
A kind of antivirus medicine for birds is characterized in that being formed by following portions by weight proportion raw material mixed preparing: Herba Andrographis 340g, Radix Isatidis 180g, Herba Houttuyniae 140g, Fructus Forsythiae 100g, Rhizoma Acori Graminei 50g, Herba Pogostemonis 40g, Venenum Bufonis 20g, Borneolum Syntheticum 60g, Rhizoma Phragmitis 40g, Gypsum Fibrosum 30g.
With following various raw materials: cross the 20-80 mesh sieve behind Herba Andrographis, Radix Isatidis, Herba Houttuyniae, Fructus Forsythiae, Rhizoma Acori Graminei, Herba Pogostemonis, Venenum Bufonis, Borneolum Syntheticum, Rhizoma Phragmitis, the Gypsum Fibrosum pulverize separately; Take by weighing Herba Andrographis 340g by weight, Radix Isatidis 180g, Herba Houttuyniae 140g, Fructus Forsythiae 100g, Rhizoma Acori Graminei 50g, Herba Pogostemonis 40g, Venenum Bufonis 20g, Borneolum Syntheticum 60g, Rhizoma Phragmitis 40g, Gypsum Fibrosum 30g; Uniform mixing.Other is with embodiment 1.
Embodiment 5
A kind of antivirus medicine for birds is characterized in that being formed by following portions by weight proportion raw material mixed preparing: Herba Andrographis 270g, Radix Isatidis 180g, Herba Houttuyniae 140g, Fructus Forsythiae 100g, Rhizoma Acori Graminei 70g, Herba Pogostemonis 60g, Venenum Bufonis 30g, Borneolum Syntheticum 40g, Rhizoma Phragmitis 80g, Gypsum Fibrosum 50g.
With following various raw materials: cross the 20-80 mesh sieve behind Herba Andrographis, Radix Isatidis, Herba Houttuyniae, Fructus Forsythiae, Rhizoma Acori Graminei, Herba Pogostemonis, Venenum Bufonis, Borneolum Syntheticum, Rhizoma Phragmitis, the Gypsum Fibrosum pulverize separately; Take by weighing Herba Andrographis 270g by weight, Radix Isatidis 180g, Herba Houttuyniae 140g, Fructus Forsythiae 100g, Rhizoma Acori Graminei 70g, Herba Pogostemonis 60g, Venenum Bufonis 30g, Borneolum Syntheticum 40g, Rhizoma Phragmitis 80g, Gypsum Fibrosum 50g; Uniform mixing.Other is with embodiment 1.
Embodiment 6
A kind of antivirus medicine for birds is characterized in that being formed by following portions by weight proportion raw material mixed preparing: Herba Andrographis 280g, Radix Isatidis 180g, Herba Houttuyniae 120g, Fructus Forsythiae 120g, Rhizoma Acori Graminei 60g, Herba Pogostemonis 50g, Venenum Bufonis 20g, Borneolum Syntheticum 60g, Rhizoma Phragmitis 60g, Gypsum Fibrosum 50g.
With following various raw materials: cross the 20-80 mesh sieve behind Herba Andrographis, Radix Isatidis, Herba Houttuyniae, Fructus Forsythiae, Rhizoma Acori Graminei, Herba Pogostemonis, Venenum Bufonis, Borneolum Syntheticum, Rhizoma Phragmitis, the Gypsum Fibrosum pulverize separately; Take by weighing Herba Andrographis 280g by weight, Radix Isatidis 180g, Herba Houttuyniae 120g, Fructus Forsythiae 120g, Rhizoma Acori Graminei 60g, Herba Pogostemonis 50g, Venenum Bufonis 20g, Borneolum Syntheticum 60g, Rhizoma Phragmitis 60g, Gypsum Fibrosum 50g; Uniform mixing.Other is with embodiment 1.
Embodiment 7
A kind of antivirus medicine for birds is characterized in that being formed by following portions by weight proportion raw material mixed preparing: Herba Andrographis 180g, Radix Isatidis 80g, Herba Houttuyniae 70g, Fructus Forsythiae 30g, Rhizoma Acori Graminei 10g, Herba Pogostemonis 10g, Venenum Bufonis 5g, Borneolum Syntheticum 30g, Rhizoma Phragmitis 30g, Gypsum Fibrosum 20g.
With following various raw materials: cross the 20-80 mesh sieve behind Herba Andrographis, Radix Isatidis, Herba Houttuyniae, Fructus Forsythiae, Rhizoma Acori Graminei, Herba Pogostemonis, Venenum Bufonis, Borneolum Syntheticum, Rhizoma Phragmitis, the Gypsum Fibrosum pulverize separately; Take by weighing Herba Andrographis 180g by weight, Radix Isatidis 80g, Herba Houttuyniae 70g, Fructus Forsythiae 30g, Rhizoma Acori Graminei 10g, Herba Pogostemonis 10g, Venenum Bufonis 5g, Borneolum Syntheticum 30g, Rhizoma Phragmitis 30g, Gypsum Fibrosum 20g; Uniform mixing.Other is with embodiment 1.
Embodiment 8
A kind of antivirus medicine for birds is characterized in that being formed by following portions by weight proportion raw material mixed preparing: Herba Andrographis 400g, Radix Isatidis 200g, Herba Houttuyniae 150g, Fructus Forsythiae 120g, Rhizoma Acori Graminei 80g, Herba Pogostemonis 80g, Venenum Bufonis 30g, Borneolum Syntheticum 80g, Rhizoma Phragmitis 90g, Gypsum Fibrosum 70g.
With following various raw materials: cross the 20-80 mesh sieve behind Herba Andrographis, Radix Isatidis, Herba Houttuyniae, Fructus Forsythiae, Rhizoma Acori Graminei, Herba Pogostemonis, Venenum Bufonis, Borneolum Syntheticum, Rhizoma Phragmitis, the Gypsum Fibrosum pulverize separately; Take by weighing Herba Andrographis 400g by weight, Radix Isatidis 200g, Herba Houttuyniae 150g, Fructus Forsythiae 120g, Rhizoma Acori Graminei 80g, Herba Pogostemonis 80g, Venenum Bufonis 30g, Borneolum Syntheticum 80g, Rhizoma Phragmitis 90g, Gypsum Fibrosum 70g; Uniform mixing.Other is with embodiment 1.
During use, this medicine is added hello birds in the feedstuff by 0.5%, promptly can prevent multiple viral disease, upper respiratory tract infection and anemopyretic cold, enumerate experiment and application example below and be summarized as follows:
1, to the experiment of newcastle disease (ND)
The present invention can be made as high, medium and low multiple concentration, as joining by the medicine that embodiment 7 configuration proportions are made in the chick embryo fibroblast (CEE) of cultivating 24h, inoculation NDV IV is after cultivating 12h again, with dimethyl diaminophenazine chloride dyestuff absorptionmetric determination CEF activity, estimate the influence of medicine on cell proliferation of the present invention and opposing viral infection thereof in inoculation back 72h with this.The measurement result explanation can significantly promote CEE propagation with medicine of the present invention in high concentration (1600 μ g/ml), inferior high concentration (1200 μ g/ml), and inferior high concentration significantly promotes the infection of CEF opposing NDV.In addition medicine of the present invention is joined among the CEF that cultivates 24h with 3 kinds of orders with NDVI, IV system: add earlier and add after adding medicine of the present invention, virus and medicament mixed of the present invention behind virus inoculation behind the medicine of the present invention, the first virus inoculation, the tissue culture infective dose (TCID50) of 72h mensuration NDV I system and the HI of NDV IV system tire behind virus inoculation, to estimate the influence of medicine of the present invention to the NDV infection cell.The result shows: medicine of the present invention fashionablely has good inhibition effect to NDV adding prior to virus.
2, the clinical efficacy of newcastle disease is tested
| Numbering | Group | Sickness rate % | Mortality rate % | The course of disease |
| A | Positive controls | 96.7(29/30) | 96.7(29/30) | 7 |
| B | Healthy group | 0(0/30) | 0(0/30) | 0 |
| C | The ribavirin therapy group | 83.3(25/30) | 16.6(5/30) | 6 |
| D | Matched group of the present invention | 93.3(28/30) | 6.67(2/30) | 7 |
| E | Existing " WENDU KESAN " matched group | 90.0(27/30) | 23.0(7/30) | 7 |
[0039]Experimental result shows, analyzes by statistics, and medicine group of the present invention and ribavirin matched group difference is P＜0.01 very significantly, treatment group and the existing also very remarkable P of " WENDU KESAN " matched group difference＜0.05.
3, adopt medicine of the present invention that Embryo Gallus domesticus artificial challenge and infectious laryngotracheitis are carried out inhibition test; result of the test shows; medicine of the present invention is very high to the protective rate that Embryo Gallus domesticus infects the strong poison of infectious laryngotracheitis virus; show that this medicine has very strong inhibitory action to infectious laryngotracheitis virus, concrete grammar is:
Academy of agricultural sciences, effluent south veterinary institute provides infectious laryngotracheitis virus, and available from the big city chicken house, 60 ages in days are without the LTI immunity with growing chickens in test.
Experiment for the first time is with 45 of chickens, 30 of test group, 15 of matched groups, with every pipe freeze-dried vaccine with the dilution of 10mlPBS liquid, hole injection inoculation 0.2ml under every chicken (test group and matched group) larynx mucosa cut and the socket of the eye.Experiment for the second time is with 50 of chickens, 35 of test group, and 15 of matched groups, inoculation is through go down to posterity infectious laryngotracheitis (1: 10 diluent of the allantocherion) 0.2ml of rejuvenation of twice Embryo Gallus domesticus.After the test group inoculation, use medicine of the present invention, logotype 7 days, matched group is not taken medicine, and observes under identical raising condition.
Morbidity and curative effect determinate standard: the chicken of inoculative infection laryngotracheitis gets rid of head, cough dyspnea, and moist rale, is covered with mucus and bleeder for falling ill at larynx mucosa flushing, swelling.Transference cure after the medication, spirit, appetite recover normal person and are recovery from illness; Death person is invalid.
Experimental result is as shown in the table:
Experiment for the first time, matched group be all morbidities in 96 hours behind virus inoculation, the course of disease 11 days, and test group is not fallen ill, preventive effect 100%.Experiment for the second time, test group and matched group be all morbidities in 72 hours behind virus inoculation, the course of disease 11 days.Dead 4 of test group is survived 31, and cure rate is 88.6%, and mortality rate is 11.4%; Dead 13 of matched group, mortality rate is 86.7%.There were significant differences for test group and matched group result, shows that this antiviral drugs has good prevention effect to virosis.
Investigate and analyse from clinical efficacy: experimental therapy was used, wherein more than 30000 chicken treated altogether by 9 units, and average cure rate is 92.5%, and minimum is 75%, is up to 97%.Medicine of the present invention is to the artificial challenge and the clinical therapeutic efficacy test of infectious laryngotracheitis virus; the result show its to the chicken protective rate of artificial challenge's infectious laryngotracheitis virus more than 95%, show that medicine of the present invention has very strong inhibitory action to infectious laryngotracheitis virus.
Above-mentioned detailed description of antivirus medicine for birds and preparation method thereof being carried out with reference to embodiment; be illustrative rather than determinate; can list several embodiment according to institute's limited range; therefore in the variation and the modification that do not break away under the general plotting of the present invention, should belong within protection scope of the present invention.
Claims (2)
1. an antivirus medicine for birds is characterized in that being formed by following portions by weight proportion raw material mixed preparing: Herba Andrographis 18-40 part, Radix Isatidis 8-20 part, Herba Houttuyniae 7-15 part, Fructus Forsythiae 3-12 part, Rhizoma Acori Graminei 1-8 part, Herba Pogostemonis 1-8 part, Venenum Bufonis 0.5-3 part, Borneolum Syntheticum 3-8 part, Rhizoma Phragmitis 3-9 part, Gypsum Fibrosum 2-7 part.
2. the preparation method of an antivirus medicine for birds is characterized in that carrying out as follows:
With following various raw materials: cross the 20-80 mesh sieve behind Herba Andrographis, Radix Isatidis, Herba Houttuyniae, Fructus Forsythiae, Rhizoma Acori Graminei, Herba Pogostemonis, Venenum Bufonis, Borneolum Syntheticum, Rhizoma Phragmitis, the Gypsum Fibrosum pulverize separately; Take by weighing Herba Andrographis 18-40 part by weight, Radix Isatidis 8-20 part, Herba Houttuyniae 7-15 part, Fructus Forsythiae 3-12 part, Rhizoma Acori Graminei 1-8 part, Herba Pogostemonis 1-8 part, Venenum Bufonis 0.5-3 part, Borneolum Syntheticum 3-8 part, Rhizoma Phragmitis 3-9 part, Gypsum Fibrosum 2-7 part; Uniform mixing.
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| CN101129165B (en) * | 2007-10-12 | 2011-11-16 | 唐自坦 | Pure feed additive of plants |
| CN102526354B (en) * | 2012-01-11 | 2014-05-07 | 河南天纳图生物科技有限公司 | Chinese medicament for viral diseases |
| CN102657708A (en) * | 2012-06-02 | 2012-09-12 | 哈尔滨工业大学 | Anti-influenza medicine with immune regulating function and preparation method thereof |
| CN103300225A (en) * | 2013-06-09 | 2013-09-18 | 广州格拉姆生物科技有限公司 | Chinese herbal medicine ultra-fine powder feed additive with antiviral and antibacterial effects, and preparation method thereof |
| CN103583808B (en) * | 2013-11-21 | 2015-07-01 | 史占彪 | Preparation method of beverage concentrated solution capable of preventing chicken bird flu, helping digestion and strengthening physique |
| CN103860931A (en) * | 2014-03-19 | 2014-06-18 | 湖州天健兽药有限公司 | Medicine for preventing and treating swine fever |
| CN104435838A (en) * | 2014-12-01 | 2015-03-25 | 郑州后羿制药有限公司 | Antiviral traditional Chinese medicine for livestock and poultry and preparation method thereof |
| CN113827688B (en) * | 2021-11-17 | 2022-03-25 | 山西农业大学 | Traditional Chinese medicine composition for reducing mortality of newcastle disease |
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| CN1546149A (en) * | 2003-11-28 | 2004-11-17 | 张晴龙 | Effervescence tablet of antiviral medicine and its preparation process |
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| CN1546149A (en) * | 2003-11-28 | 2004-11-17 | 张晴龙 | Effervescence tablet of antiviral medicine and its preparation process |
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| 翁亚彪等.半仿生提取 修复肠粘膜——护膜先.兽药市场指南2005 12.2005,2005(12),15-16. * |
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