CN107661416A - A kind of traditional Chinese medicine extraction compound pharmaceutical for preventing and treating livestock and birds respiratory disease - Google Patents
A kind of traditional Chinese medicine extraction compound pharmaceutical for preventing and treating livestock and birds respiratory disease Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/35—Caprifoliaceae (Honeysuckle family)
- A61K36/355—Lonicera (honeysuckle)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/19—Acanthaceae (Acanthus family)
- A61K36/195—Strobilanthes
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
- A61K36/233—Bupleurum
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/31—Brassicaceae or Cruciferae (Mustard family), e.g. broccoli, cabbage or kohlrabi
- A61K36/315—Isatis, e.g. Dyer's woad
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/34—Campanulaceae (Bellflower family)
- A61K36/346—Platycodon
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/38—Clusiaceae, Hypericaceae or Guttiferae (Hypericum or Mangosteen family), e.g. common St. Johnswort
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/484—Glycyrrhiza (licorice)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
- A61K36/534—Mentha (mint)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
- A61K36/539—Scutellaria (skullcap)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/63—Oleaceae (Olive family), e.g. jasmine, lilac or ash tree
- A61K36/634—Forsythia
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/70—Polygonaceae (Buckwheat family), e.g. spineflower or dock
- A61K36/704—Polygonum, e.g. knotweed
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/85—Verbenaceae (Verbena family)
- A61K36/855—Clerodendrum, e.g. glorybower
Abstract
The invention discloses a kind of traditional Chinese medicine extraction compound pharmaceutical for preventing and treating livestock and birds respiratory disease, it is made up of the raw material of following weight percentage：Honegsukle flower P.E 10~15%, Baical Skullcap root P.E 3~7%, forsythia suspense extraction 23~27%, Hypericum Perforatum P.E 10~15%, isatis root extract 10~15%, Folium Isatidis extract 10~15%, Radix Platycodonis extract 1~6%, mint extract 1~6%, bupleurum extract 5~10%, licorice 1~5%, the mass percent sum of above-mentioned raw materials is 100%.The traditional Chinese medicine extraction compound pharmaceutical of the prevention and treatment livestock and birds respiratory disease of the present invention, the potential safety hazard caused by chemical antimicrobial DP finish can be reduced, curative effect really, respiratory symptom can effectively be alleviated, respiratory system tissue damage is eliminated, suitable for the livestock and poultry respiratory tract infection as caused by virus and bacterium etc..
Description
Technical field
The invention belongs to pharmaceutical technology field, is related to a kind of medicine for preventing and treating livestock and birds respiratory disease, specifically relates to
A kind of and traditional Chinese medicine extraction compound pharmaceutical for preventing and treating livestock and birds respiratory disease.
Background technology
Livestock and poultry respiratory systemic disease mainly causes sneeze, cough and snore etc. a series of based on respiratory symptom
The general name of disease, autumn and winter are mainly in, bacterium and virus are the main pathogens for causing such disease, cause Production of Livestock and Poultry
Energy reduces, efficiency of feed utilization and daily gain decline, or even cause the death of livestock and poultry, has a strong impact on the development of aquaculture industry of China, right
The economy of aquaculture causes huge loss.It there is no specific drug can effectively preventing livestock and poultry respiratory tract infection at present.It is although domestic
Carry out immunoprophylaxis to such disease based on vaccine outside, but appearance and vaccine immunity due to pathogen variant it is improper or
The problems caused by failure, vaccine immunity is often set not reach desired effect.
In the case where vaccine is undesirable to livestock and birds respiratory disease immune effect, antibiotic and chemicals in prevention and
The application treated in such disease is more and more extensive, largely easily produces drug resistance problems using antibiotic and chemicals.
And as the proposition of the concepts such as ecologic breeding and pollution-free food, China's regulation are forbidden to use veterinary clinic in feed and food
Middle antibiotics and chemical classes medicine.Therefore, it is badly in need of developing noresidue, small toxicity and the antibiotic replacement having no drug resistance
Product.And the action effect of the antiviral and antibacterial of Chinese medicine is good, toxic side effect is small, can not only improve immunity of organisms, also has and promotees
The advantages that entering growth, improving production performance makes people see hope.Especially in recent years, Chinese medicine is used for controlling in veterinary clinic
Treat and prophylactic generation is more and more concerned.Therefore, Chinese medicine preparation is developed to be used to prevent and treat livestock and poultry respiratory
Disease is significant.
The content of the invention
For above-mentioned problems of the prior art and defect, it is an object of the invention to provide one kind to prevent and treat
The Chinese medicine preparation of livestock and birds respiratory disease, the Chinese medicine preparation have dispelling wind and heat from the body, and clearing lung, relieving asthma is clearing heat and detoxicating, reducing pharyngeal swelling
Effect, cure mainly the livestock and birds respiratory disease caused by virus or bacterium Simple infection or mixed infection.
It is that a kind of Chinese medicine for preventing and treating livestock and birds respiratory disease carries to realize that foregoing invention purpose uses technical scheme
Compound pharmaceutical is taken, is made up of the raw material of following weight percentage：Honegsukle flower P.E 10~15%, Baical Skullcap root P.E 3~
7%th, forsythia suspense extraction 23~27%, Hypericum Perforatum P.E 10~15%, isatis root extract 10~15%, folium isatidis extraction
Thing 10~15%, Radix Platycodonis extract 1~6%, mint extract 1~6%, bupleurum extract 5~10%, licorice 1~
5%, the mass percent sum of above-mentioned raw materials is 100%.
It is of the present invention prevention and treatment livestock and birds respiratory disease traditional Chinese medicine extraction compound pharmaceutical optimization formula by
The raw material of following weight percentage is made：Honegsukle flower P.E 11~14%, Baical Skullcap root P.E 4~6%, forsythia suspense extraction 24
~26%, Hypericum Perforatum P.E 11~14%, isatis root extract 11~14%, Folium Isatidis extract 11~14%, balloonflower root
Extract 2~5%, mint extract 2~5%, bupleurum extract 7~9%, licorice 2~4%, the matter of above-mentioned raw materials
It is 100% to measure percentage sum.
It is of the present invention prevention and treatment livestock and birds respiratory disease traditional Chinese medicine extraction compound pharmaceutical optimum formula by
The raw material of following masses percentage is made：Honegsukle flower P.E 13%, Baical Skullcap root P.E 5%, forsythia suspense extraction 25%, pass through leaf company
Stick up extract 13%, isatis root extract 13%, Folium Isatidis extract 13%, Radix Platycodonis extract 4%, mint extract 3%, bavin
Hu extract 8%, licorice 3%.
The preparation method of the Chinese medicine preparation of prevention and treatment livestock and birds respiratory disease of the present invention：In taking in formula
Medicament extract, mixed according to the ratio of formula and loose powder agent is made；Also the auxiliary material dextrin of appropriate ratio can be added in loose powder agent, is mixed
It is even, particle is made in granulator；Loose powder agent can be also configured to every milliliter of solution containing 1 gram of crude drug, add appropriate auxiliary material
Sodium benzoate, stir evenly, stand, sterilizing, traditional Chinese medicine extraction compound pharmaceutical oral liquid is made.
The traditional Chinese medicine extraction compound pharmaceutical of the present invention for being used to treat livestock and birds respiratory disease, is to be based on traditional Chinese medical science pharmacology
By with reference to modern Chinese herbal medicine Study on Modernization achievement, being made according to traditional Chinese medicine combination principle.The present invention has dispelling wind and heat from the body, clearing lung-heat
Relieving asthma, it is clearing heat and detoxicating, the effect of reducing pharyngeal swelling, cure mainly the livestock and poultry caused by virus or bacterium Simple infection or mixed infection and exhale
Inhale tract disease.
Honeysuckle, the capsule of weeping forsythia are main ingredient in the prescription of the present invention, and two medicine phases must be to use, clearing heat and detoxicating, dispelling wind and heat from the body, Xin Liang solutions
Table, Synergistic；There are chlorogenic acid, glucoside, flavonoids and volatile oil component contained therein anti-multiple pathogenic microorganisms to make
With.Radix Isatidis, folium isatidis, radix scutellariae and hypericum perforatum are adjuvant, have clearing heat and detoxicating, diathermanous outgoing and other effects, wherein Radix Isatidis
There is antibacterial, antiendotoxin and the effect of certain raising immunity of organisms with the indigo, indigo red of main component in folium isatidis etc., it is main
Control febrile virulent maculae and abscess of throat；Radix scutellariae clearing heat-fire, for cough with lung heat caused by warm disease；Contain Hypericum Chinense in hypericum perforatum
Element, there is resisiting influenza virus effect.Peppermint, radix bupleuri are adjutant, peppermint dispelling wind-heat, refresh oneself, cure mainly and are had a headache caused by warm disease
And hot eyes；Radix bupleuri have and inducing diaphoresis in, soothing the liver and rising Yang the effect of.Radix glycyrrhizae is moistened the lung and relieve the cough；Balloonflower root has clearing lung and eliminating the phlegm and apocenosis
Detumescence, carries the effect of medicine enters lung channel, is to make medicine altogether.All medicines share, there is dispelling wind and heat from the body, clearing heat and detoxicating, clearing lung, relieving asthma, relieving sore-throat disappears
The effect of swollen.
The traditional Chinese medicine extraction compound pharmaceutical of prevention and treatment livestock and birds respiratory disease prepared by the present invention, it is anti-to reduce chemistry
It potential safety hazard caused by mushroom medicine, curative effect really, can effectively alleviate respiratory symptom, eliminate respiratory system tissue damage,
Suitable for the livestock and poultry respiratory tract infection as caused by virus and bacterium etc..
Special instruction：The traditional Chinese medicine extraction compound pharmaceutical occurred in text, it is the treatment livestock and birds respiratory disease of the present invention
Medicine.
Had shown that through pharmacology test and animal experiment research, the traditional Chinese medicine extraction compound pharmaceutical have antiviral, antibacterial and
The effect of improving immunity of organisms, has preferable preventive and therapeutic action to viral and bacterial livestock and poultry respiratory tract infection.
The thin-layer chromatography and high-efficiency liquid chromatography method for detecting established can detect to the quality of Chinese medical extract compound preparation.
Brief description of the drawings
Fig. 1 is radix scutellariae thin-layer chromatogram；
Fig. 2 is capsule of weeping forsythia thin-layer chromatogram；
Fig. 3 is the Solution H PLC measure figures of scutelloside reference substance
Fig. 4 is the HPLC measure figures of Baical Skullcap root P.E in traditional Chinese medicine extraction compound pharmaceutical of the present invention；
Fig. 5 is the Solution H PLC measure figures of negative controls.
Embodiment
It is expanded on further traditional Chinese medicine extraction compound pharmaceutical of the present invention by the following examples.Following examples are mainly used
It is of the invention in further illustrating, rather than limitation the scope of the present invention.
In order to which substance, validity and the security of the present invention is better described, following pharmacodynamics test has been substantially carried out.
Chinese medical extract is weighed by following proportionings：Honegsukle flower P.E 15g, Baical Skullcap root P.E 5g, forsythia suspense extraction 23g,
Hypericum Perforatum P.E 10g, isatis root extract 10g, Folium Isatidis extract 15g, Radix Platycodonis extract 6g, mint extract 3g,
Bupleurum extract 10g, licorice 3g.Said medicine is well mixed, and loose powder agent, total amount 100g is made.
Preparation method：The Chinese medical extract in formula is taken, fully mixes and loose powder agent is made；Also appropriate ratio can be added in loose powder agent
The auxiliary material dextrin of example, mix, particle is made in granulator；Loose powder agent can be also configured to every milliliter of solution containing 1 gram of crude drug,
Appropriate auxiliary material sodium benzoate is added, is stirred evenly, is stood, sterilizing, traditional Chinese medicine extraction compound pharmaceutical oral liquid is made.
1. the inhibitory action that traditional Chinese medicine extraction compound pharmaceutical is bred to newcastle disease virus
80 piece of 9 age in days health chicken embryo is randomly divided into low dosage prevention group (containing crude drug 0.5g/mL), middle dosage prevention group
(containing crude drug 1g/mL), high dose prevention group (containing crude drug 2g/mL), low dose therapy group (containing crude drug 0.5g/mL), middle dosage are controlled
Totally 8 groups, every group for the treatment of group (containing crude drug 1g/mL), high-dose therapy group (containing crude drug 2g/mL), positive controls and negative control group
10 pieces of chicken embryos.Prevention group first injects Chinese medicine preparation 0.2mL in chick embryo allantoic cavity, is placed in 37 DEG C of incubators and cultivates 60min, inoculates
Newcastle disease vaccine poison (ewcastle disease La Sota strains) 0.2mL；Treatment group first in allantoic cavity vaccine inoculation poison 0.2mL, is placed in 37 DEG C of temperature
60min is cultivated in case, then injects Chinese medicine 0.2mL；Positive controls vaccinate malicious 0.2mL, place and are cultivated in 37 DEG C of incubators
96h, discard chicken embryo dead in 24h per 12h according to egg once, collect dead chicken embryo after 24h, be put at 4 DEG C and preserve, gather
Allantoic fluid, measure hemagglutinative titer (HA).Result of the test is shown in Table 1, and as seen from table, traditional Chinese medicine extraction compound pharmaceutical can reduce new city
The hemagglutinative titer of epidemic disease poison, significant effect (P ﹤ 0.01 or P when middle dosage (containing crude drug 1g/mL) and high dose (containing crude drug 2g/mL)
﹤ 0.05).
The traditional Chinese medicine extraction compound pharmaceutical of table 1 is to newcastle disease virus proliferation function effect
Note：Significantly (P ﹤ 0.05), different capitalizations represent that difference is extremely notable to different lowercase letter indication differences in same column
(P ﹤ 0.01).
2. vaccine immunity enhancing experiment
50 25 age in days pigeons for meat are randomly divided into 5 groups：Low dose oral liquid group (+0.5g/mL medicines are immunized in NDV), middle dosage mouth
Take liquid group (NDV be immunized+1g/mL medicines), high dose oral liquid group (+2g/mL medicines are immunized in NDV), negative control group (not being immunized),
Positive controls (NDV is immunized).28 ages in days start drinking water administration, continuous 7d, and 31 ages in days carry out vaccine immunity, work as respectively at immune
My god, immune rear every group of 7d, 14d, 21d, 28d randomly selects 5 by wing venous blood collection, separates serum, measure coagulation antibody effect
Valency.Result of the test is shown in Table 2, rear 14d, 21d and 28d is being immunized, high dose group and middle dose group antibody level are above positive right
According to group, middle dose group significant difference (P ﹤ 0.05) compared with the antibody level of other each groups, high dose group and positive controls it
Between significant difference (P ﹤ 0.05), the antibody level of low dose group and positive controls keeps in identical level substantially, and difference is not
Significantly.Result above shows that the medicine group of each concentration can strengthen NDV immune effect, with the Immune-enhancing effect of middle dose group
Effect is best.
Table 2NDV vaccine immunities strengthen result of the test
Note：Significantly (P ﹤ 0.05), different capitalizations represent that difference is extremely notable to different lowercase letter indication differences in same column
(P ﹤ 0.01).
3. influence of the traditional Chinese medicine extraction compound pharmaceutical to pigeon for meat Development of Immune Organs
1) to the influence of pigeon for meat Immune Organs Index
Result of the test is shown in Table 3, and the spleen and thymus gland shoot formation difference of each group be not notable；Bursa of farbricius shoot formation is in high agent
Difference is not notable between amount group, low dose group and positive controls, middle dose group significant difference (P ﹤ compared with positive controls
0.05)。
Influence of the Chinese medical extract preparation of table 3 to pigeon for meat Immune Organs Index
Note：The different alphabetical person's difference of same column is extremely significantly or significant difference (P ﹤ 0.05).
2) to the influence of pigeon for meat immune organ and tissue development
The spleen splenic corpuscle of each group is clear in structure, and arteria linenalis peripheral lymphoid sheath profile is obvious, splenic corpuscle endolymph cell row
Row are close, and the lymphocyte quantity of middle dose group, low dose group and positive controls is obvious compared with the lymphocyte quantity of control group
Increase；High dose group, middle dose group, low dose group and the arrangement of positive controls lobules of thymus endolymph cell are close, and lymph
Cell quantity is higher than control group.
4. Experimental infection
120 healthy chicks are randomly divided into following 3 groups, every group 40, infected for infectious bronchitis virus real
Test, virus infection amount is that 0.5mL/ only (contains 2.65 × 103ELD50), it is every each 0.5mL that medicine, which gavages dosage, and 12h gavages 1
It is secondary.(1) prevention group：24h virus inoculations 0.5mL, successive administration 3d after administration.(2) treatment group：The 24h inoculations disease before administration
Malicious 0.5mL, successive administration 3d.(3) control group：24h virus inoculations 0.5mL before physiological saline is gavaged, gavages physiology within every 12 hours
Salt solution 0.5mL, continuous 3d.Clinical symptoms, death condition and the cut open inspection change of each group chicken are observed, and records result.As a result show,
Infectious bronchitis virus control group engenders clinical symptoms after to malicious 48h；After 60h, other 2 groups there is clinical condition
Shape.Cough, sneezing, has a running nose and tracheae rale, spiritual depressed, anorexia, amount of drinking water increase.It is morbidity between 96~128h
Peak period.The visible tracheae of cut open inspection has serous effusion, kidney is pale, enlargement, renal tubule and ureter because of uric acid mineralization and
Expansion.X is carried out between treatment group, prevention group and control group2Examine, as a result treatment group, the prevention group equal difference compared with control group
Significantly, illustrate that traditional Chinese medicine extraction compound pharmaceutical plays the role of obvious suppression virus.Chick morbidity number and the incidence of disease are shown in Table 4.
The infectious bronchitis virus Experimental infection chick incidence of table 4
Note：* significant difference (the P compared with control group<0.05)
5. bacterium body outer suppressioning test
Using microdetermination traditional Chinese medicine extraction compound pharmaceutical to EHEC, salmonella typhimurium, killing property bar more
Family name bacillus, Streptococcusagalactiae, the minimum bactericidal concentration of staphylococcus aureus (being reference culture), respectively 15.6mg/mL,
125mg/mL, 31.2mg/mL, 15.6mg/mL, 31.2 mg/mL, it follows that traditional Chinese medicine extraction compound pharmaceutical is 5 kinds to more than
Bacterium has good In-vitro Inhibitory Effect.
6. the acute toxicity test of traditional Chinese medicine extraction compound pharmaceutical
1) to the toxicity test of mouse
Kunming mouse 40,18~22g, male and female half and half, is randomly divided into four groups, gavages 4g/mL, 2g/mL, 1g/ respectively
ML and 0.5g/mL traditional Chinese medicine extraction compound pharmaceutical 0.5mL, per 12h once, Continuous Observation 2 weeks.As a result：Each group mouse is acute
Growing state is good during toxicity test, is showed no abnormal response, no dead generation.According to acute toxicity grading criteria, Chinese medicine
Extractive compound preparation category non-poisonous material.
2) to the toxicity test of chicken embryo
Traditional Chinese medicine extraction compound pharmaceutical is taken to be made into 4g/mL containing crude drug, 2g/mL, 1g/mL and 0.5g/mL decoction, every group 6
Piece 9 age in days health chicken embryos, each allantoic cavity injection are diluted to the traditional Chinese medicine extraction compound pharmaceutical medicine of each concentration, every piece of chicken embryo
0.2mL, while control group is set, place in 37 DEG C of incubators and cultivate 96h, discard chicken embryo dead in 24h per 12h according to egg once,
Dead chicken embryo, observes chicken embryo death quantity after collection 24h.As a result show, the equal normal incubation of each group chicken embryo is not found to shell is gone out
There is toxicity to chicken embryo.
7. quality control testing
1) thin-layer chromatography (TLC)
Take traditional Chinese medicine extraction compound pharmaceutical, reference《Republic of China Veterinary Pharmacopoeia》(2015) methods described is to radix scutellariae
Glycosides, forsythin carry out TLC detections.As a result Fig. 1 and Fig. 2 are seen, Fig. 1 is radix scutellariae thin-layer chromatogram, the scutelloside 3. of 1. sample 2. in figure
The Baical Skullcap root P.E of negative control 4.；Fig. 2 is capsule of weeping forsythia thin-layer chromatogram, the forsythin 4. of 1. forsythia suspense extraction, 2. negative control 3. in figure
Sample.In test sample chromatogram, the fluorescent spot of same color is shown on position corresponding with standard reference material and extract chromatogram
Point.
2) HPLC detects baicalin
Reference《Republic of China Veterinary Pharmacopoeia》(2015) content assaying method of scutelloside is detected, prepared yellow
The solution of a kind of reed mentioned in ancient books glycosides sample, standard reference material and negative controls, sample introduction HPLC measure, records peak area respectively, calculates in sample
The content of scutelloside.Result of the test is shown in Fig. 3 (scutelloside reference substance), Fig. 4 (traditional Chinese medicine extraction compound pharmaceutical of the present invention) and Fig. 5
(negative control).Scutelloside peak retention time in test sample and the peak in standard reference material are consistent, without radix scutellariae
There is not scutelloside peak in identical retention time in negative controls, the peak adjacent with other point of the scutelloside peak in test sample
From good, and without in radix scutellariae negative controls to the noiseless phenomenon of scutelloside.
8. clinical practice example
Embodiment 1
Traditional Chinese medicine extraction compound pharmaceutical by Honegsukle flower P.E 15g, Baical Skullcap root P.E 5g, forsythia suspense extraction 23g, pass through leaf
Forsythia suspense extraction 10g, isatis root extract 10g, Folium Isatidis extract 15g, Radix Platycodonis extract 6g, mint extract 3, radix bupleuri carry
Take thing 10g, licorice 3g.Said medicine is well mixed, and loose powder agent, total amount 100g is made.
Embodiment 2：
Traditional Chinese medicine extraction compound pharmaceutical by Honegsukle flower P.E 10g, Baical Skullcap root P.E 7g, forsythia suspense extraction 27g, pass through leaf
Forsythia suspense extraction 15g, isatis root extract 12g, Folium Isatidis extract 12g, Radix Platycodonis extract 1g, mint extract 6g, radix bupleuri
Extract 5g, licorice 5g.Said medicine is well mixed, and loose powder agent is made.
Xianyang raiser opens ××：More than 1000 only 120 age in days kinds pigeon for meat, morbidities on March 9th, 2016；Symptom：In dove group
There is " chuckleing " sound in part, rale, stretches neck, faces upward head, mouth breathing, lassitude, poor appetite, arrange green loose stool；Use every time
50g medicines, dissolved with appropriate cold boiled water water using preceding, freely drunk for dove group, continuously taken three days, twice daily, every every
Secondary dosage is 0.05g.Dove group full recovery health.
Embodiment 3：
Traditional Chinese medicine extraction compound pharmaceutical by Honegsukle flower P.E 11g, Baical Skullcap root P.E 3g, forsythia suspense extraction 24g, pass through leaf
Forsythia suspense extraction 14g, isatis root extract 15g, Folium Isatidis extract 14g, Radix Platycodonis extract 3g, mint extract 5g, radix bupleuri
Extract 7g, licorice 4g.Said medicine is well mixed, and loose powder agent is made.
Baoji raiser's favour ××：More than 2000 laying hens, 240 ages in days, morbidity on December 11st, 2016；Symptom：Chicken shows
For cough, breathe, have " chuckle " and sound, egg production decline, produce lopsided egg, husky lime-preserved egg.The visible throat's mucous membrane of sporadic deaths chicken cut open inspection
On there is faint yellow coagulum to adhere to, be not easy to wipe, arrange green loose stool；400g medicines are used every time, using preceding with appropriate cold boiled water water
Dissolving, freely drinks, continuous five days, twice daily, every each dosage is 0.2g for chicken group.Chicken group more than 95% recovers strong
Health.
Embodiment 4：
Traditional Chinese medicine extraction compound pharmaceutical by Honegsukle flower P.E 12g, Baical Skullcap root P.E 4g, forsythia suspense extraction 25g, pass through leaf
Forsythia suspense extraction 13g, isatis root extract 14g, Folium Isatidis extract 13g, Radix Platycodonis extract 5g, mint extract 4g, radix bupleuri
Extract 9g, licorice 1g.Said medicine is well mixed, and loose powder agent is made.
Shanglou raiser king's ××：Cultivate sow 100, the remainder of growing and fattening pigs 800,2 months 2017, due to Changes in weather,
Uneven in temperature, wherein poor appetite, high fever, cough, asthma occur for the remainder of growing and fattening pigs 50, and expiratory dyspnea, hair are thick disorderly, spirit is heavy
It is strongly fragrant.Appropriate cold boiled water water dissolving before being used every time with 95g, and electrolysis multidimensional is properly added, add in water fountain, continuous drink-service is used
Traditional Chinese medicine extraction compound pharmaceutical five days, twice daily, every each dosage is 5g.Swinery more than 95% gets well.Nothing is faced
The bed full comedicine amount of symptom halves prevention, then does not occur above-mentioned disease.
Embodiment 5：
Traditional Chinese medicine extraction compound pharmaceutical by Honegsukle flower P.E 13g, Baical Skullcap root P.E 6g, forsythia suspense extraction 26g, pass through leaf
Forsythia suspense extraction 12g, isatis root extract 13g, Folium Isatidis extract 15g, Radix Platycodonis extract 4g, mint extract 1g, radix bupleuri
Extract 8g, licorice 2g.Said medicine is well mixed, and loose powder agent is made.
Embodiment 6：
Traditional Chinese medicine extraction compound pharmaceutical by Honegsukle flower P.E 14g, Baical Skullcap root P.E 7g, forsythia suspense extraction 27g, pass through leaf
Forsythia suspense extraction 11g, isatis root extract 11g, Folium Isatidis extract 11g, Radix Platycodonis extract 6g, mint extract 2g, radix bupleuri
Extract 6g, licorice 5g.Said medicine is well mixed, and loose powder agent is made.
It is described above, it is only the preferred embodiments of the present invention, therefore change in the case where not departing from present general inventive concept and repair
Change, should belong within protection scope of the present invention.
Claims (3)
1. a kind of traditional Chinese medicine extraction compound pharmaceutical for preventing and treating livestock and birds respiratory disease, it is characterised in that by following weight
The raw material of percentage is made：Honegsukle flower P.E 10~15%, Baical Skullcap root P.E 3~7%, forsythia suspense extraction 23~27%, pass through leaf company
Stick up extract 10~15%, isatis root extract 10~15%, Folium Isatidis extract 10~15%, Radix Platycodonis extract 1~6%, peppermint
Extract 1~6%, bupleurum extract 5~10%, licorice 1~5%, the mass percent sum of above-mentioned raw materials is 100%.
2. the traditional Chinese medicine extraction compound pharmaceutical of prevention and treatment livestock and birds respiratory disease according to claim 1, its feature
It is, is made up of the raw material of following weight percentage：Honegsukle flower P.E 11~14%, Baical Skullcap root P.E 4~6%, capsule of weeping forsythia extraction
Thing 24~26%, Hypericum Perforatum P.E 11~14%, isatis root extract 11~14%, Folium Isatidis extract 11~14%, balloonflower root
Extract 2~5%, mint extract 2~5%, bupleurum extract 7~9%, licorice 2~4%, the quality hundred of above-mentioned raw materials
It is 100% to divide than sum.
3. the traditional Chinese medicine extraction compound pharmaceutical of prevention and treatment livestock and birds respiratory disease according to claim 1, its feature
It is, is made up of the raw material of following weight percentage：Honegsukle flower P.E 13%, Baical Skullcap root P.E 5%, forsythia suspense extraction 25%, pass through
Leaf forsythia suspense extraction 13%, isatis root extract 13%, Folium Isatidis extract 13%, Radix Platycodonis extract 4%, mint extract 3%, bavin
Hu extract 8%, licorice 3%.
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