CN104739963A - Preparation method of Chinese medicinal micro-ecological oral preparation for preventing chicken colibaciuosis - Google Patents
Preparation method of Chinese medicinal micro-ecological oral preparation for preventing chicken colibaciuosis Download PDFInfo
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Abstract
The invention discloses a preparation method of a Chinese medicinal micro-ecological oral preparation for preventing chicken colibaciuosis. The preparation method comprises the following steps: preparing honeysuckle, humifuse euphorbia herb, Chinese pulsatilla root, coptis root, fel bovis, indigowood root, plantain herb, astragalus, pomegranate bark and liquoric root; performing ultrafine grinding on the Chinese medicinal herbs, adding an ethanol solution, boiling with strong fire, preserving heat with slow fire, cooling, filtering, concentrating filtrate, adding white sugar, boiling, cooling to the normal temperature, inoculating lactobacillus, tea fungus and saccharomycetes, and fermenting for 120-168 hours till white fungus blocks grow out; III, diluting the white fungus blocks and a bacterium solution in the ratio 1:(100-300), and performing secondary fermentation for 24-48 hours to obtain the micro-ecological oral preparation. The micro-ecological oral preparation has the effects of increasing the quantity of effective microbial communities in intestinal canal, enhancing the immunological function and enhancing the disease resistance of chicken. The Chinese medicinal micro-ecological oral preparation has the beneficial effects of good prevention effect, curative ratio being up to 94.6 percent, simple preparation method, high viable content, convenience in use, environmental friendliness and freeness from residues.
Description
Technical field
The invention belongs to microbial forage additive technical field, relate to a kind of preparation method of preventing and treating the natural green fowl Traditional Chinese drug microecology oral agents of chicken colibacillosis specifically.
Background technology
Escherichia coli (Escherichia coli, E.coli) are Gram-negative, the bacillus not forming spore, and size is generally 0.4 ~ 0.7 μm × 2 ~ 3 μm, the blunt circle in two ends, is dispersed in or in pairs, most of bacterial strain has whole body flagellum, energy wave motion alive, variant atrichia can not move.Escherichia coli are amphimicrobian type bacterium, well-grown on ordinary culture medium, and optimum temperature is 37 DEG C, and optimum pH is 7.2 ~ 7.4.It is one of normal flora in people and most homoiothermic animal intestinal, and after humans and animals birth, a few hours can enter digestive tract back segment propagation, adjoint throughout one's life, and constantly disseminate in surrounding through feces.Therefore escherichia coli are in environmental health and food hygiene, be often used as the Testing index that feces directly or indirectly pollutes.Mainly containing O, K, H 3 kinds according to the different bacillus coli antigens of surface antigen, is the material base of this bacterium Serotype Identification.The escherichia coli O antigen determined has 173 kinds, and K antigen has 80 kinds, and H antigen has 56 kinds, and the serotype that occurring in nature may exist can reach tens thousand of kinds, result in it and prevent and treat aspect difficulty very greatly, but the serotype of Escherichia coli is limited.
Chicken colibacillosis is the infectious disease caused by the avian pathogenic Escherichia coli of some serotype, comprise a series of diseases such as e. coli septicemia, granuloma, airsacculitis, perihepatitis, Swollen head syndrome, peritonitis, salpingitis, synovitis, panophthalmitis and omphalitis, this disease is one of major reason of Embryo Gallus domesticus and chickling death.This disease mainly occurs in intensive poultry-farm, various birds regardless of kind, sex, age in days, all to this bacterium susceptible.Particularly the most easily there is this disease in poult, as being in the environment of filth, crowded, moist, improper ventilation, supercool overheated or the temperature difference is very large, toxic and harmful long-term existence, feeding and management is lacked of proper care, malnutrition (particularly the shortage of vitamin) and pathogenic microorganism (as mycoplasma and virus) infect cause stress wait the generation that all can impel primary disease.This is prevalent in the intestinal of nature and various animal, but has and pathogenic only have 10%-15%; It is the main route of transmission of this disease that fecal pollution and hatching egg pollute.It is depressed that chicken colibacillosis clinical symptoms shows as the sick chicken spirit of their early stage, loss of appetite or absolutely useless, and two wings are sagging, drink for slightly increasing, eyelid swelled, nasal secretions, and breathe and have rale, cockscomb is dark violet redness; Part chicken is drawn yellow green, canescence loose stool, is sometimes mixed with blood, feather faecal contamination under perianal and abdomen.The pathological change of chicken colibacillosis: fibrinous pericarditis, pericardial effusion is muddy, thicken; Cellulosic perihepatitis, liver enlargement, surface have fibrinous exudate; Cellulosic peritonitis, the ascites that abdominal cavity has quantity not wait and cellulose exudate, or its exudate is full of between each internal organs in abdominal cavity; The portion's adhesion of yolk peritonitis fimbriae of uterine tube, follicle are fallen into abdominal cavity and are caused extensive peritonitis; Oophoritis, salpingitis fallopian tube are congested, hemorrhage, and it is hardening that theca folliculi is congested, follicle distortion, variable color (in bronzing or pitchy) have, and yolk is thinning.
At present, China's chicken colibacillosis drug resistance is seriously complicated, and nearly all antibacterials have all produced drug resistance, and its drug resistance is based on multidrug resistant, no longer rest on several antibiotic crossing drug resistants in same class.The infection that escherichia coli Resistant strain causes not only has the trend increased clinically, and drug resistance is also constantly changing, and the appearance of bacillus coli multiple Resistant strain has had a strong impact on the clinical therapeutic efficacy of this disease.Family's avian escherichia coli produces the speed of drug resistance and is exceedingly fast, main and the current antibiotic abuse of China, poultry farming environment, breeding way and technical merit are closely related.Raiser is using antibiotic as the colibacillary Main Means of control, but in practical application, because drug sensitive test popularity rate is low, medication blindness is large, and random escalated dose, low dosage life-time service, does not note rotation medication, causes escherichia coli drug resistance serious.As time goes on, the resistant rate of chicken colibacillosis significantly rises, and the fast increasing of multiple antibiotic resistant strain, Antibiotic Resistance is broadening.The generation of drug resistance, causes drug effect to decline even invalid, and medicine controls difficulty and increases.The abuse of medicine causes the imbalance of microorganism species in body in addition, is also the common risk factor of colibacillosis one.
Chinese herbal medicine be the Chinese nation for thousands of years produce struggle in the knowledge of natural environment, nature remodeling wisdom crystallization, be the guarantee of our national prosper.It is natural drug; obtain the corresponding various composition and ability that conform and resist various infringement during evolution; set up the regulating system (BRM) of self; the intrinsic medicine resistance ability and the BRM material contained by Chinese medicine can be strengthened or watch for animals; and its property of medicine is gentle; toxic and side effects is little even to have no side effect, and these are all that chemicals can not be compared.Chinese veterinarian's dialectical treatmert theory think this disease be by invading in epidemic disease poison, exuberant lung-stomach heat, the blood stasis stagnation of QI cause, treatment by Chinese herbs is appropriate to the occasion based on heat-clearing and toxic substances removing, removing heat from blood, dampness medicine.Much Chinese medicine all has the effect of anti-inflammation, and use treatment by Chinese herbs disease not easily to produce drug resistance, nontoxic or low toxicity, noresidue or low-residual, some Chinese medicines also have the effect regulating body function and extra-nutrition concurrently simultaneously.Therefore Chinese medicine is selected will to be the development trend that aquaculture is prevented and cured diseases to treat chicken colibacillosis.
And the Tiny ecosystem oral agents being prepared into and preventing and treating chicken colibacillosis that Chinese medicine is fermented together with microorganism, have no report in prior art.
Summary of the invention
The object of the invention is to the deficiency for above technology, solve the drug resistance of current colibacillosis and the problem of antibiotic remains, there is provided a kind of preparation method with control chicken colibacillosis Traditional Chinese drug microecology oral agents, simple, the easy to use, safe environmental protection of this preparation method, pure ecology, noresidue, viable bacteria content are high.
For achieving the above object, the preparation method that the present invention adopts is as follows:
Prevent and treat a preparation method for chicken colibacillosis Traditional Chinese drug microecology oral agents, it is characterized in that: it is prepared according to the following steps: 1, weigh Flos Lonicerae, Herba Euphorbiae Humifusae, the Radix Pulsatillae, Rhizoma Coptidis, Fel Bovis seu Bubali, Radix Isatidis, Herba Plantaginis, the Radix Astragali, Pericarpium Granati, Radix Glycyrrhizae; 2, except Fel Bovis seu Bubali, each Study on Supermicro-pulverization of Traditional Chinese Drugs is added alcoholic solution intense fire and boils, slow fire is incubated, cold filtration, concentrated filtrate, adds white sugar and boils, inoculating lactobacillus, red tea fungus and yeast after room temperature are down in cooling, through 120 ~ 168h fermentation to growing white truffle; 3, by white truffle and bacterium liquid, dilute by 1:100 ~ 300, after ferment in second time 24 ~ 48h, obtain micro-ecological oral tonic.
Described a kind of preparation method of preventing and treating chicken colibacillosis Traditional Chinese drug microecology oral agents, is characterized in that: the Chinese medicine weighing following weight portion: Flos Lonicerae 80 ~ 100, Herba Euphorbiae Humifusae 80 ~ 100, the Radix Pulsatillae 80 ~ 100, Rhizoma Coptidis 80 ~ 100, Fel Bovis seu Bubali 60 ~ 80, Radix Isatidis 60 ~ 80, Herba Plantaginis 40 ~ 60, the Radix Astragali 40 ~ 60, Pericarpium Granati 30 ~ 40, Radix Glycyrrhizae 20 ~ 30.
Described a kind of preparation method of preventing and treating chicken colibacillosis Traditional Chinese drug microecology oral agents, it is characterized in that: except Fel Bovis seu Bubali, weigh the Chinese medicine of weight portion, micronizing, add the alcoholic solution of 50% ~ 70% of Chinese medicine gross weight 8 ~ 10 times amount, soak 30 ~ 60min, intense fire boils, slow fire insulation 30 ~ 60min, cooling Floor 12 filtered through gauze; Filtering residue adds the water of 6 ~ 8 times amount again, and intense fire boils, slow fire insulation 30 ~ 60min, cooling Floor 12 filtered through gauze, merges twice filtrate, concentration and recovery ethanol, detecting in concentrated solution concentrated without stopping during ethanol with ethanol detector, in concentrated solution, adding Fel Bovis seu Bubali simultaneously.
Described a kind of preparation method of preventing and treating chicken colibacillosis Traditional Chinese drug microecology oral agents, it is characterized in that: in units of 100 Kg concentrated solutions, add white sugar 10 ~ 15Kg, and boil 3 ~ 5min, be cooled to room temperature, inoculating lactobacillus, red tea fungus, yeast (commercially available) preparation 20 ~ 25Kg, setting fermentation temperature 28 ~ 35 DEG C, standing for fermentation cultivates 120 ~ 168h, to pH6.5 ~ 7.0 grow white truffle.
Described a kind of preparation method of preventing and treating chicken colibacillosis Traditional Chinese drug microecology oral agents, it is characterized in that: by white truffle and bacterium liquid, be blended into 5% ~ 10% cold Sugar water dilution by 1:100 ~ 300, fermentation temperature 20 ~ 35 DEG C, after ferment in second time 24-48h, obtain micro-ecological oral tonic.
Described a kind of preparation method of preventing and treating chicken colibacillosis Traditional Chinese drug microecology oral agents, is characterized in that: step 2 is carried out in aseptic fermenting cellar.
Traditional Chinese drug microecology oral agents of the present invention has increases profitable strain quantity in intestinal, improve immunologic function, strengthen the effects such as chicken body premunition, and preparation method is simple, easy to use, noresidue.Beneficial effect preventive effect of the present invention is good, and cure rate reaches 94.6%, viable bacteria content height after measured content 8 × 10
8cfu/g.
The minimal inhibitory concentration (MIC) of Traditional Chinese drug microecology oral agents of the present invention and the mensuration of minimum bactericidal concentration (MBC) and drug sensitive test, measure first time fermentation liquid and ferment in second time liquid respectively, this test bacterial strain uses therefor is escherichia coli O
78(CVCC1418) purchased from China Veterinery Drug Inspection Office.Test tube doubling dilution is adopted to measure minimal inhibitory concentration (MIC), concrete operations are as follows: get aseptic small test tube 9 of jumping a queue, often add the broth medium 1mL made in pipe, Chinese medicine 1mL is added at 1 pipe, mix with micropipettor, draw 1mL to mix to the 2nd pipe, successively to the 7th pipe, abandon it from the 7th pipe sucking-off 1mL mixed liquor, 8th pipe does positive control (add bacterium liquid and do not add Chinese medicine), 9th pipe does negative control (add Chinese medicine and do not add bacterium liquid), now the concentration of 1 to No. 7 pipe inner liquid medicines is equivalent to 500mg/mL respectively, 250mg/mL, 125mg/mL, 62.5mg/mL, 31.25mg/mL, 15.63mg/mL, 7.81mg/mL crude drug concentration, add the bacterium liquid 0.1mL diluted to 1-8 pipe, the 9th pipe does not add bacterium liquid, but adds 0.1mL LB broth bouillon, cultivate 20h under each group of test tube is placed in 37 DEG C of conditions, observe and record result, compare with matched group, have the Guan Zewei of flocculent deposit to have bacterial growth, the pipe without flocculent deposit is the suppressed tubulation of bacterial growth, using perusal without the lowest concentration of drug of flocculent deposit pipe as the minimal inhibitory concentration (MIC) of this medicine, there is no unified standard at present because the MIC of Chinese herbal medicine to antibacterial is domestic, this test (is extremely sensitive when MIC≤15.63mg/mL crude drug concentration with reference to the standard of similar test as criterion, be medium sensitivity when MIC is in 15.63 ~ 62.5mg/mL crude drug concentration, when MIC is 62.5 ~ 250.00 mg/mL crude drug concentration for more responsive, be insensitive when MIC>250.00 mg/mL crude drug concentration) measure MIC after, an inoculating loop is got successively again from each tube culture having no bacterial growth, be inoculated on the plain agar flat board of not pastille, spread bacterium liquid gently out, cultivate 24 h, observe with or without bacterial growth, in plain agar flat board for 37 DEG C, the clump count of growth is less than the medicinal liquid least concentration of 5 bacterium colonies, is MBC, drug sensitive test adopts disk diffusion method, measures antibacterial circle diameter, reference standard drug sensitive test result, judges the sensitivity of medicine.Record and the results are shown in Table 1.
Table 1 MIC, MBC and drug sensitive test result
| MIC(mg/mL) | MBC (mg/mL) | Antibacterial circle diameter (mm) | Sensitivity | |
| 1 fermentation liquid | 15.63 | 31.25 | 18.3 | High responsive |
| 2 fermentation liquids | 31.25 | 62.5 | 12.7 | Middle sensitivity |
As can be seen from Table 1, first time fermentation liquid MIC15.63 mg/mL, second time fermentation liquid MIC31.25mg/mL be that this is consistent with standard drug sensitive test result for high sensitivity and middle sensitivity according to similar test standard, illustrate that the fungistatic effect of Traditional Chinese drug microecology oral agents of the present invention is fine, solve the problem that antibiotic drug resistance is serious.
Traditional Chinese drug microecology oral agents of the present invention is to the therapeutic test of artificial challenge's chicken colibacillosis: experimental chicken is that north, Anhui partridge chickens 300 is raised to 12 ages in days, weigh after checking body condition phenomenon without exception, by its random packet, be divided into negative control group (not counteracting toxic substances not administration), positive controls (counteracting toxic substances not administration), antibiotic matched group (enrofloxacin), 1 fermentation group, 2 fermentation groups, often organize each 60 chickens; Escherichia coli O
78(CVCC1418) after rejuvenation, except negative control group, all the other respectively organize thoracic muscle injection concentration is 5.6 × 10
8the bacterium liquid 0.2mL/ of cfu/ml only, 1 fermentation group, in counteracting toxic substances morbidity relief, it freely drinks 2 fermentation groups, antibiotic matched group gavages after counteracting toxic substances morbidity that to allow it freely drink enrofloxacin soluble powder concentration of aqueous solution be 0.2g/ml, negative and positive controls is each freely drinks cold boiled water, be used in conjunction 5 days, Continuous Observation 5 days; The standard of curative effect evaluation, Continuous Observation 5 days, curative effect determinate standard is: spirit, diet, defecation recover normal, and symptom disappears be judged to healing completely; Spirit, diet, defecation take a favorable turn, and symptom obviously alleviates, and are judged to effectively; Symptom is even dead without improvement, and cuing open inspection has the typical escherichia coli pathological changes such as obvious pericarditis, perihepatitis, and it is invalid to be judged to.Calculate mortality rate according to dead chicken number, the results are shown in Table 2.
Table 2 Traditional Chinese drug microecology oral liquid is to artificial challenge and colibacillosis therapeutic effect
| Group | Quantity (only) | Death toll (only) | Mortality rate (%) | Survival number (only) | Cure rate (%) |
| 1 fermentation group | 60 | 4 | 6.7 | 56 | 93.3 |
| 2 fermentation groups | 60 | 5 | 8.3 | 55 | 91.7 |
| Antibiotic matched group | 60 | 17 | 28.3 | 43 | 71.7 |
| Positive controls | 60 | 44 | 73.3 | 16 | 26.7 |
| Negative control group | 60 | 0 | 0 | 60 | 100 |
1 fermentation and the rear medicinal liquid cure rate of 2 fermentations are obviously better than antibiotic matched group as can be seen from Table 2, illustrate that escherichia coli have certain drug resistance to this antibiosis, and Traditional Chinese drug microecology oral liquid of the present invention does not produce drug resistance, there is good therapeutic effect, each group of cure rate Analysis of variance find 1 fermentation group and 2 fermentation group differences not remarkable, this does not conform to the result of the MIC recorded, illustrate that the fungistatic effect in In Vitro Bacteriostasis and chicken body is different, the testing result of MIC can provide reference for clinical treatment, wherein, antifungal mechanism in chicken body needs further to be studied.
Detailed description of the invention
embodiment 1
Prevent and treat a preparation method for chicken colibacillosis Traditional Chinese drug microecology oral agents, comprise the following steps:
Weigh the Chinese medicine of following weight portion (kg): Flos Lonicerae 80, Herba Euphorbiae Humifusae 80, the Radix Pulsatillae 80, Rhizoma Coptidis 80, Radix Isatidis 60, Herba Plantaginis 40, the Radix Astragali 40, Pericarpium Granati 30, Radix Glycyrrhizae 20 micronizing, add the alcoholic solution of 50% of Chinese medicine gross weight 8 times amount, soak 30min, intense fire boils, slow fire insulation 30min, cooling Floor 12 filtered through gauze; Filtering residue adds the water of 6 times amount again, and intense fire boils, slow fire insulation 30min, and cooling Floor 12 filtered through gauze, merges twice filtrate, concentration and recovery ethanol, detecting in concentrated solution concentrated without stopping during ethanol, adding Fel Bovis seu Bubali 60kg with ethanol detector.
The preparation of Tiny ecosystem oral agents: add white sugar 10kg in units of 100 Kg concentrated solutions, and boil 3min, be cooled to room temperature, inoculating lactobacillus, red tea fungus, yeast (commercially available) preparation 20kg, setting fermentation temperature 28 DEG C, standing for fermentation cultivates 168h, to pH6.5 grows white truffle, by white truffle and bacterium liquid 10kg, be blended into the 5% cold Sugar water dilution of 1000kg, fermentation temperature 20 DEG C, after ferment in second time 48h, obtains micro-ecological oral tonic.
embodiment 2
Prevent and treat a preparation method for chicken colibacillosis Traditional Chinese drug microecology oral agents, comprise the following steps:
Weigh the Chinese medicine of following weight portion (kg): Flos Lonicerae 100, Herba Euphorbiae Humifusae 100, the Radix Pulsatillae 100, Rhizoma Coptidis 100, Radix Isatidis 80, Herba Plantaginis 60, the Radix Astragali 60, Pericarpium Granati 40, Radix Glycyrrhizae 30 micronizing, add the alcoholic solution of 70% of Chinese medicine gross weight 10 times amount, soak 60min, intense fire boils, slow fire insulation 60min, cooling Floor 12 filtered through gauze; Filtering residue adds the water of 8 times amount again, and intense fire boils, slow fire insulation 60min, and cooling Floor 12 filtered through gauze, merges twice filtrate, concentration and recovery ethanol, detecting in concentrated solution concentrated without stopping during ethanol, adding Fel Bovis seu Bubali 80kg with ethanol detector.
The preparation of Tiny ecosystem oral agents: add white sugar 15Kg in units of 100 Kg concentrated solutions, and boil 5min, be cooled to room temperature, inoculating lactobacillus, red tea fungus, yeast (commercially available) preparation 25Kg, setting fermentation temperature 5 DEG C, standing for fermentation cultivates 120h, to pH7.0 grows white truffle.By white truffle and bacterium liquid 5kg, be blended into the 10% cold Sugar water dilution of 1500kg, fermentation temperature 35 DEG C, after ferment in second time 48h, obtains micro-ecological oral tonic.
embodiment 3
In August, 2014, Northern Huaihe River Anhui chicken house broiler 10000, immune programme for children carries out the immunity of the epidemic disease such as bird flu, newcastle to chicken group routinely.Chicken is mined massively and gets that ground is flat supports, autogamy feedstuff, chicken population density is larger, improper ventilation, raise to during 7 weeks age due to more than continuously everyday unhappy heat add that bedding and padding are not changed in time, part chicken performance spirit, inappetence, feather is fluffy and disorderly, respiratory murmur is abnormal, arrange yellowish green or draw white light stool, in fragmentary dead, after morbidity, poultry raiser amoxicillin, the former powder spice of norfloxacin is all without positive effect, still have continuous death, amount to dead 100, cut open inspection liver, spleen, the organs such as kidney have congestion in various degree, the obvious enlargement of liver and color is light, matter is crisp, there is the exudative pseudomembrane of yellow-white on surface, what have comprises a large amount of cellulosic material around conscience.Intestinal tube mucosa and cloaca mucosa hemorrhage point, large intestinal wall is thickening, diffusivity is congested, and Fallopian tube mucosa is congested, hemorrhage, examining change, tentatively suspecting it is coli-infection according to clinical symptoms with cuing open.
With 1 fermentation liquid 100kg of the embodiment of the present invention 2, after adding the cold Sugar water fermentation 24h of 10% of 10000kg, chicken is allowed freely to drink, coupling 5 days, observe chicken group situation, all during medication, chicken is after imbibable administration, there is not symptoms such as having loose bowels, its mental status, appetite, breathing wait until that progressively recovering normal person all belongs to healing, calculate cure rate, after the medication course for the treatment of dead 534, survive 9366, cure rate is 94.6%.
In August, 2014, Northern Huaihe River Anhui chicken house laying hen 5000, because many unhappy heat everyday adds that feces is not cleared up in time continuously, part chicken performance spirit, inappetence, feather is fluffy and disorderly, respiratory murmur is abnormal, arrange yellowish green or draw white light stool, in fragmentary dead, after morbidity, poultry raiser ampicillin, the former powder spice of Cephalothin is all without positive effect, still have continuous death, amount to dead 20, cut open inspection liver, spleen, the organs such as kidney have congestion in various degree, the adhesion of fimbriae of uterine tube portion, follicle falls into abdominal cavity and causes extensive peritonitis, fallopian tube is congested, hemorrhage, theca folliculi is congested, follicle is out of shape, it is hardening that variable color (in bronzing or pitchy) has, yolk is thinning, change is examined with cuing open according to clinical symptoms, preliminary suspection is coli-infection.
With 1 fermentation liquid 100kg of the embodiment of the present invention 1, after adding the cold Sugar water fermentation 48h of 5% of 10000kg, chicken is allowed freely to drink, coupling 7 days, observe chicken group situation, all during medication, chicken is after imbibable administration, there is not symptoms such as having loose bowels, its mental status, appetite, breathing wait until that progressively recovering normal person all belongs to healing, calculate cure rate, after the medication course for the treatment of dead 228, survive 4652, cure rate is 95.3%.
Claims (6)
1. prevent and treat a preparation method for chicken colibacillosis Traditional Chinese drug microecology oral agents, it is characterized in that: it is prepared according to the following steps: one, extracting honeysuckle, Herba Euphorbiae Humifusae, the Radix Pulsatillae, Rhizoma Coptidis, Fel Bovis seu Bubali, Radix Isatidis, Herba Plantaginis, the Radix Astragali, Pericarpium Granati, Radix Glycyrrhizae; Two, except Fel Bovis seu Bubali, each Study on Supermicro-pulverization of Traditional Chinese Drugs is added alcoholic solution intense fire and boils, slow fire is incubated, cold filtration, concentrated filtrate, adds white sugar and boils, inoculating lactobacillus, red tea fungus and yeast after room temperature are down in cooling, through 120 ~ 168h fermentation to growing white truffle; Three, by white truffle and bacterium liquid, dilute by 1:100 ~ 300, after ferment in second time 24 ~ 48h, obtain micro-ecological oral tonic.
2. a kind of preparation method of preventing and treating chicken colibacillosis Traditional Chinese drug microecology oral agents according to claim 1, is characterized in that: the Chinese medicine weighing following weight portion: Flos Lonicerae 80 ~ 100, Herba Euphorbiae Humifusae 80 ~ 100, the Radix Pulsatillae 80 ~ 100, Rhizoma Coptidis 80 ~ 100, Fel Bovis seu Bubali 60 ~ 80, Radix Isatidis 60 ~ 80, Herba Plantaginis 40 ~ 60, the Radix Astragali 40 ~ 60, Pericarpium Granati 30 ~ 40, Radix Glycyrrhizae 20 ~ 30.
3. a kind of preparation method of preventing and treating chicken colibacillosis Traditional Chinese drug microecology oral agents according to claim 1, it is characterized in that: except Fel Bovis seu Bubali, weigh the Chinese medicine of weight portion, micronizing, add the alcoholic solution of 50% ~ 70% of Chinese medicine gross weight 8 ~ 10 times amount, soak 30 ~ 60min, intense fire boils, slow fire insulation 30 ~ 60min, cooling Floor 12 filtered through gauze; Filtering residue adds the water of 6 ~ 8 times amount again, and intense fire boils, slow fire insulation 30 ~ 60min, cooling Floor 12 filtered through gauze, merges twice filtrate, concentration and recovery ethanol, detecting in concentrated solution concentrated without stopping during ethanol with ethanol detector, in concentrated solution, adding Fel Bovis seu Bubali simultaneously.
4. a kind of preparation method of preventing and treating chicken colibacillosis Traditional Chinese drug microecology oral agents according to claim 1, it is characterized in that: in units of 100 Kg concentrated solutions, add white sugar 10 ~ 15Kg, and boil 3 ~ 5min, be cooled to room temperature, inoculating lactobacillus, red tea fungus, yeast (commercially available) preparation 20 ~ 25Kg, setting fermentation temperature 28 ~ 35 DEG C, standing for fermentation cultivates 120 ~ 168h, to pH6.5 ~ 7.0 grow white truffle.
5. a kind of preparation method of preventing and treating chicken colibacillosis Traditional Chinese drug microecology oral agents according to claim 1, it is characterized in that: by white truffle and bacterium liquid, 5% ~ 10% cold Sugar water dilution is blended into by 1:100 ~ 300, fermentation temperature 20 ~ 35 DEG C, after ferment in second time 24-48h, obtain micro-ecological oral tonic.
6. a kind of preparation method of preventing and treating chicken colibacillosis Traditional Chinese drug microecology oral agents according to claim 1, is characterized in that: step 2 is carried out in aseptic fermenting cellar.
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| CN106074812A (en) * | 2016-08-29 | 2016-11-09 | 兴文县鸿程农业生态科技开发有限责任公司 | A kind of Chinese medicine compound for preventing and treating bacterial disease of fowl and application |
| CN107412457A (en) * | 2017-05-17 | 2017-12-01 | 仁怀市五马镇佛山土鸡养殖场 | A kind of Chinese medicine for preventing and treating chicken colibacillosis |
| CN108783076A (en) * | 2018-06-28 | 2018-11-13 | 浦北县清华生态养鸡专业合作社 | Prevent the preparation method of chicken colibacillosis feed |
| CN109480088A (en) * | 2018-12-14 | 2019-03-19 | 河北维尔利动物药业集团有限公司 | It is a kind of prevent and treat Chinese ephedra chicken escherichia coli infection nutrition peck brick and preparation method thereof |
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