CN103860715B - A kind of compound Chinese medicine extract and application thereof preventing and treating coccidiosis of domestic fowls - Google Patents
A kind of compound Chinese medicine extract and application thereof preventing and treating coccidiosis of domestic fowls Download PDFInfo
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Abstract
The present invention relates to animal compound technical field of Chinese medicines, specifically disclose a kind of compound Chinese medicine extract preventing and treating coccidiosis of domestic fowls and its preparation method and application.Compound Chinese medicine extract of the present invention is prepared from through certain technique according to certain proportioning by Radix Dichroae, Herba Artemisiae Annuae, Herba Agrimoniae, Cortex Phellodendri, the Radix Pulsatillae, Radix Sophorae Flavescentis Six-element medical material.Gained drug extract of the present invention is applicable to the control of coccidiosis of domestic fowls, effectively prevent because of the drug residue caused by antibiotic and drug resistance.The present invention is simultaneously according to the poultry physiological feature feature that especially poultry reproductive tract are short, the problem of absorption and digestion is difficult to for Chinese medicine cellulose, therefore medicine is made Chinese medicine extract, by drinking water administration, facilitate and be convenient to absorb, the object for the treatment of can be played rapidly, there is good economic benefit and social benefit.
Description
Technical field
The present invention relates to animal compound technical field of Chinese medicines, particularly relate to a kind of compound Chinese medicine extract preventing and treating coccidiosis of domestic fowls and its preparation method and application.
Background technology
Parasitic disease is the disease that poultry is very common, harm is extremely serious.Especially coccidiosis of domestic fowls, provisions fowl industry brings serious economic loss.After Chickens Infected coccidiosis, a large amount of enterocyte is impaired, hemorrhage, and severe patient is dead, and this disease is mainly following three aspects to the harm that chicken group causes: (1) causes large quantities of morbidity and the death of chicken; (2) chicken growth promoter is hindered; (3) price of deed is reduced.The M & M of this disease is higher.In intensive culture factory, its sickness rate is up to 50% ~ 70%, and case fatality rate can up to 80%.It is reported, the loss that the whole world causes because of chicken coccidiosis every year reaches 500,000,000 pounds, if coccidiosis causes intestinal downright bad, indirect loss will reach 1,000,000,000 pounds.China's year raising broiler is about 30 ~ 6,000,000,000 plumages, and average every plumage consumes anticoccidial drug by 0.2 ~ 0.3 yuan of calculating, then the annual expense for anticoccidial drug is just up to 6 ~ 1,800,000,000 yuan.Along with the development of intensive poultry husbandry and chicken strengthen chemotherapeutic resistance, the harm of primary disease will be on the rise, and can significantly increase chicken intestinal diorder Salmonella infection during eimeria tenella infection, extend Salmonella enteritidis to the environmental emission time.Therefore the existence of coccidiosis, the chain negative effect no matter brought with regard to itself or it, all that direct harm humans is healthy and poultry husbandry development.Therefore, how preventing and treating primary disease is one of important topic of current poultry husbandry.
At present, medicine remains the Main Means of control coccidiosis of domestic fowls.A lot of anticoccidial drug such as sulfanilamide quinoline Dumb beautiful jade sodium, monensin, Salinomycin, Maduramicin etc. all have certain effect to control primary disease, but often treat thorough not, and easily recur after drug withdrawal, cause weightening finish to be slowed down, price of deed rate reduces; Simultaneously due to the extensive use of various anticoccidial drug, coccidiosis is made to create drug resistance, scope of medication is caused to reduce, dosage strengthens, the application of strong dose thing not only creates toxic action to body, and easily cause residual in livestock products, bring food-safety problem, this has become the focus that current people pay close attention to.Facts have proved, the relative Western medicine of Chinese medicine has plurality of advantages, and as little in toxic and side effects, use safety, drug withdrawal not easily recur, not easily produce drug resistance and drug residue etc.Therefore research and produce a kind of novel efficient, low toxicity, safe Chinese medicine preparation, a new way will be opened up for chemoprophylaxis and treatment coccidiosis of domestic fowls.
Summary of the invention
For the deficiencies in the prior art, the present invention aims to provide compound Chinese medicine extract of a kind of prevention and therapy coccidiosis of domestic fowls and its preparation method and application, by selecting suitable medical material and proportioning thereof and formulating the extracting method of its patent medicine, reach safe and effective treatment and prevention poultry against coccidia disease and the object making it be useful in apply clinically.
In order to achieve the above object, the present invention is by the following technical solutions:
A compound Chinese medicine extract for prevention and therapy coccidiosis of domestic fowls, is prepared from by the Chinese crude drug of following weight:
Radix Dichroae 15-30 part, has preventing the attack (or recurrence) of malaria, pest-resistant, refrigeration function;
Herba Artemisiae Annuae 40-80 part, clear deficiency-heat thoroughly, removing heat from blood, except steaming, removes and steams, preventing the attack (or recurrence) of malaria;
Herba Agrimoniae 10-20 part, astringing to arrest bleeding, dysentery relieving, parasite killing, is widely used in various hemorrhage card;
Cortex Phellodendri 10-20 part, nature and flavor bitter cold, has heat clearing and damp drying, and pathogenic fire purging removes steams, effect of detoxification sore treatment;
Radix Pulsatillae 10-20 part, has heat-clearing and toxic substances removing, eliminating pathogenic heat from blood to cure dysentery, effect of removing dampness and killing parasites;
Radix Sophorae Flavescentis 10-20 part, has heat clearing and damp drying, effect of parasite killing.
Best, described compound Chinese medicine extract is prepared from by the Chinese crude drug of following weight: Radix Dichroae 25 parts, Herba Artemisiae Annuae 60 parts, Herba Agrimoniae 15 parts, Cortex Phellodendri 15 parts, the Radix Pulsatillae 15 parts, Radix Sophorae Flavescentis 10 parts.
The preparation method step of described compound Chinese medicine extract is as follows:
Take each Chinese crude drug of described weight portion, mixing, add each Chinese crude drug gross weight 2-3 water soaking of 60-65 DEG C doubly after 1 hour, feed liquid, in 92 DEG C of reflux, extract, 10 hours, then concentrates, when being concentrated into 3 times of each Chinese crude drug gross weight by the water adding each Chinese crude drug gross weight 12 times again, leave standstill 12h, make precipitation, getting supernatant, to be evaporated to relative density be 1.15 ~ 1.20 (55-60 DEG C), spraying dry, receive powder, pulverize, cross 50 mesh sieves, mixing, to obtain final product.
The present invention is guidance according to the genesis mechanism of chinese tradition Chinese veterinarian medicine theory, China's " rarity " Chinese herbal medicine characteristic and coccidiosis of domestic fowls and poultry digestive system feature, according to the rigorous formula such as monarch, seven emotions compatibility of Chinese medicine, make it divide the work clearly, Each performs its own functions; Compound Chinese medicine extract preparation of a kind of prevention and therapy coccidiosis of domestic fowls simultaneously screened one by one in a large amount of clinical trial and preparation method thereof.Pharmacological action of the present invention: according to the dialectical theoretical principle of Chinese veterinarian, coccidiosis of domestic fowls belongs to caused by damp syndrome clinically, Radix Dichroae in the present invention, Radix Sophorae Flavescentis, Herba Artemisiae Annuae have effect of parasite killing, heat clearing and damp drying, are control poultry against coccidia; Herba Agrimoniae, Cortex Phellodendri, the Radix Pulsatillae have eliminating fire and detoxication, effect of cooling blood for hemostasis dysentery relieving, are used for the treatment of hemafecia, dysentery.All medicines coordinate the effect with parasite killing, heat clearing and damp drying, removing heat from blood, hemostasis, dysentery relieving, removing toxic substances, antiinflammatory.
Compared with prior art, advantage of the present invention is:
1, the invention provides a kind of pure Chinese medicinal compound extract preparation, efficiently solve the resistance to chemical medicine problem of current poultry against coccidia, search out a kind of novel, effective hands section solving coccidiosis of domestic fowls problem in cultivation;
2, pure Chinese medicine extract preparation, nontoxic, to poultry internal organs not damaged, and drug residue free, the poultry prod green safety produced, efficiently solves a series of food-safety problems that chemical medicine brings, and meets the consumption requirement of current compatriots to poultry prod;
3, medicine is made Chinese medicine extract, easy to use, efficiently solve the problem that poultry centering drug fiber element is difficult to absorption and digestion, easily absorb, and absorb fast, can play rapidly the object for the treatment of, curative effect time is long;
4, prevention and cure rate high, clinical trial reaches the cure rate of 94%.
Detailed description of the invention
In order to make those skilled in the art understand better and implement the present invention, further illustrate the present invention below in conjunction with specific embodiment.But following content should not be understood to the restriction to claims of the present invention request protection domain in any degree.
In following examples 1-4, Sichuan, the Chinese crude drug Radix Dichroae place of production used, Hubei, the Herba Artemisiae Annuae place of production, Hubei, the Herba Agrimoniae place of production, Cortex Phellodendri is that Cortex Phellodendri is produced, Hebei, the Radix Pulsatillae place of production, Sichuan, the Radix Sophorae Flavescentis place of production in Sichuan.
Embodiment 1
A compound Chinese medicine extract for prevention and therapy coccidiosis of domestic fowls, is prepared from by the Chinese crude drug of following weight: Radix Dichroae 15 parts, Herba Artemisiae Annuae 40 parts, Herba Agrimoniae 10 parts, Cortex Phellodendri 10 parts, the Radix Pulsatillae 10 parts, Radix Sophorae Flavescentis 10 parts.
The Chinese crude drug of above-mentioned weight is mixed, the warm water (60-65 DEG C) adding medical material gross weight 2.5 times soaks after 1 hour, the water adding medical material gross weight 12 times again 92 DEG C of heating and refluxing extraction 10 hours in the concentrated unit of extraction, then feed liquid is concentrated, when feed liquid concentrates 3 times of most medical material gross weight, feed liquid vacuum pump is evacuated to settling tank, leave standstill 12h, make precipitation, getting supernatant, to be evaporated to relative density be 1.15 ~ 1.20 (55-60 DEG C), spraying dry, receive powder, pulverize, 50 orders sieve mixing, to obtain final product.
Embodiment 2
A compound Chinese medicine extract for prevention and therapy coccidiosis of domestic fowls, is prepared from by the Chinese crude drug of following weight: Radix Dichroae 20 parts, Herba Artemisiae Annuae 50 parts, Herba Agrimoniae 13 parts, Cortex Phellodendri 10 parts, the Radix Pulsatillae 12 parts, Radix Sophorae Flavescentis 15 parts.Compound Chinese medicine extract is prepared by the preparation method of embodiment 1
Embodiment 3
A compound Chinese medicine extract for prevention and therapy coccidiosis of domestic fowls, is prepared from by the Chinese crude drug of following weight: Radix Dichroae 25 parts, Herba Artemisiae Annuae 60 parts, Herba Agrimoniae 15 parts, Cortex Phellodendri 15 parts, the Radix Pulsatillae 15 parts, Radix Sophorae Flavescentis 10 parts.Compound Chinese medicine extract is prepared by the preparation method of embodiment 1
Embodiment 4
A compound Chinese medicine extract for prevention and therapy coccidiosis of domestic fowls, is prepared from by the Chinese crude drug of following weight: Radix Dichroae 30 parts, Herba Artemisiae Annuae 80 parts, Herba Agrimoniae 20 parts, Cortex Phellodendri 20 parts, the Radix Pulsatillae 20 parts, Radix Sophorae Flavescentis 20 parts.Compound Chinese medicine extract is prepared by the preparation method of embodiment 1.
In order to determine that optimum amount proportioning combines, invention has been clinical effect trial, carrying out checking and illustrating:
Test 1
Experimental animal and feedstuff:
Select 1 age in days Luo Man egg healthy chick 600 plumage, raising of raising in cages.According to the trophic level of NRC (1994) fowl, feed without the feedstuff of anticoccidial, and free choice feeding, drinking-water.
Test egg capsule:
Purebred Eimeria Tenella egg capsule, is provided by Xinyang College of Agriculture and Forestry's parasite laboratory, and before test, artificial challenge's non-ball worm chicken is gone down to posterity 1 time, obtains test egg capsule.
Animal experiment grouping and process
Chickling 420 plumage that during 19 age in days, selective body heavy phase is near, healthy, is divided into 7 groups at random, often organizes 60 plumages, select and remain at random one group not counteracting toxic substances as a control group, every plumage chicken all oral 8 × 10 in all the other 6 groups
4individual egg capsule carries out counteracting toxic substances.After infecting the 2nd day starts administration, and I-IV group adopts the compound Chinese medicine extract that in the present invention prepared by embodiment 1-4 to treat respectively, freely drinks water to add 2g compound Chinese medicine extract whole day in every 1L water; V group is add 1g sodium sulfaquinoxaline whole day in the every 1L water of first day freely to drink water, and later every day freely drinks water to add 0.5g sodium sulfaquinoxaline whole day in every 1L water; VI group of counteracting toxic substances not administration group; VII group is not counteracting toxic substances not administration group; Each test group is isolated all separately, does not interfere with each other between each group.Successive administration is slaughtered after treating 8 days and is cutd open inspection.
Observation of curative effect:
Observe and record the incidence of each group of chicken after inoculation coccidiosis, clinical symptoms.Infect and start to check feces every day according to a conventional method for the 4th day, record hemafecia situation, cuts open inspection to dead chicken, checks caecum lesion, and statistics survival rate, rate of body weight gain, pathological changes value, egg capsule value etc., calculate anticoccidial index by Merck company standard method.Each group of therapeutic effect is in table 1:
Table 1
According to anticoccidial index (AIC) criteria for classification: AIC≤120 are invalid, 120 < AIC < 160 are effectively medium, and 160≤AIC≤180 are good, and AIC > 180 is outstanding; The anticoccidial index of compound Chinese medicine extract prepared by the present invention's four embodiments is all greater than 160, survival rate is all more than 93%, difference is extremely remarkable compared with sodium sulfaquinoxaline test group, show that prescription of the present invention has good Anti-human globulin test, but the better effects if of embodiment 3 some, so be defined as optimum proportioning combination.
In order to verify that the present invention is in conjunction with clinical therapeutic efficacy further, carry out verification experimental verification further
Test 2
Experimental animal: in October, 2012, Xinzhou District, Wuhan City chicken house 20 age in days 5000 plumage chicken generation coccidiosis, adopts the obtained compound Chinese medicine extract of sodium sulfaquinoxaline, diclazuril and embodiment 3 to carry out contrast therapy test.
Remedy measures: sodium sulfaquinoxaline group is add 1g sodium sulfaquinoxaline whole day in the every 1L water of first day freely to drink water, and later every day freely drinks water to add 0.5g sodium sulfaquinoxaline whole day in every 1L water; Diclazuril group is freely drunk water to add 1mg diclazuril whole day in every 1L water; Prescription of the present invention (embodiment 3) extract is freely drunk water to add 2g whole day in every 1L water; Continuous treatment 8 days.
Result: after medication is treated 4 days, each group of clinical symptoms is eased, extract obtained group of the present invention (embodiment 3) death toll amount is effectively controlled, and sodium sulfaquinoxaline and the dead quantity of diclazuril group are controlled after 6 days, and concrete test situation is in table 2:
Table 2
Result of the test shows, best prescription of the present invention (embodiment 3) extract has good clinical therapeutic efficacy to chicken coccidiosis, cure rate reaches 94.2%, significantly reduces the mortality rate of the chicken that falls ill, improve cure rate and effective percentage compared with chemical medicine treatment group.
Test 3
Experimental animal: in November, 2012, Xinzhou District, Wuhan City chicken house 22 age in days 4000 chicken generation coccidiosis, adopt the obtained compound Chinese medicine extract of the embodiment of the present invention 3 to carry out therapeutic test.
Remedy measures: the embodiment of the present invention 3 is extract obtained freely drinks water to add 2g whole day in every 1L water; Continuous treatment 8 days.
Result: after medication is treated 3 days, each group clinical symptoms is eased, and stop death after 4 days, concrete test situation is in table 3:
Result of the test shows again, and the compound Chinese medicine extract that optimum formula of the present invention (embodiment 3) obtains has good clinical therapeutic efficacy to chicken coccidiosis, and cure rate reaches 94%.
Claims (1)
1. a compound Chinese medicine extract for prevention and therapy coccidiosis of domestic fowls, is characterized in that, is prepared from by the Chinese crude drug of following weight:
Radix Dichroae 25 parts, Herba Artemisiae Annuae 60 parts, Herba Agrimoniae 15 parts, Cortex Phellodendri 15 parts, the Radix Pulsatillae 15 parts, Radix Sophorae Flavescentis 10 parts;
The step of the preparation method of described compound Chinese medicine extract is as follows:
Take each Chinese crude drug of described weight portion, mixing, add each Chinese crude drug gross weight 2-3 water soaking of 60-65 DEG C doubly after 1 hour, feed liquid, in 92 DEG C of reflux, extract, 10 hours, then concentrates, when being concentrated into 3 times of each Chinese crude drug gross weight by the water adding each Chinese crude drug gross weight 12 times again, leave standstill 12h, make precipitation, get supernatant be evaporated to 55-60 DEG C at relative density be 1.15 ~ 1.20, spraying dry, receive powder, pulverize, cross 50 mesh sieves, mixing, to obtain final product.
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| CN106721402A (en) * | 2015-11-19 | 2017-05-31 | 武汉市畜牧兽医科学研究所 | A kind of compound Chinese herb formula for preventing and treating laying hen peritonitis and its application |
| CN106214778B (en) * | 2016-08-31 | 2017-12-01 | 旌德县德源农业开发有限公司 | A kind of medicine for preventing and treating chicken coccidiasis and its preparation method and application |
| CN108210764A (en) * | 2016-12-13 | 2018-06-29 | 河南后羿实业集团有限公司 | A kind of Chinese medicine composition for treating chicken coccidiasis and preparation method thereof |
| CN108210610A (en) * | 2016-12-13 | 2018-06-29 | 河南后羿实业集团有限公司 | It is a kind of to be used to treat drug of coccidiosis of livestock and poultry and preparation method thereof |
| CN107982414A (en) * | 2017-11-13 | 2018-05-04 | 佛山科学技术学院 | A kind of chicken feed addictive for preventing global-worm illness |
| CN107669957B (en) * | 2017-11-24 | 2021-01-08 | 山南市乃东区农牧综合服务中心 | Compound traditional Chinese medicine extract for preventing and treating animal echinococcosis and application thereof |
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Effective date of registration: 20200107 Address after: 432600 Anlu City, Xiaogan, Hubei Patentee after: Hubei Xinhua Ecological Animal Development Co., Ltd. Address before: 430208 Hubei Province, Wuhan city Jiangxia District Jinkou Street Paper Jin Road No. 2 Patentee before: Wuhan Inst of Veterinary Science |