CN103919929A - Traditional Chinese medicine composition used for inhibiting turbot edwardsiella tarda disease - Google Patents
Traditional Chinese medicine composition used for inhibiting turbot edwardsiella tarda disease Download PDFInfo
- Publication number
- CN103919929A CN103919929A CN201410181491.4A CN201410181491A CN103919929A CN 103919929 A CN103919929 A CN 103919929A CN 201410181491 A CN201410181491 A CN 201410181491A CN 103919929 A CN103919929 A CN 103919929A
- Authority
- CN
- China
- Prior art keywords
- weight portions
- chinese medicine
- medicine composition
- turbot
- parts
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The invention provides a traditional Chinese medicine composition used for inhibiting turbot edwardsiella tarda disease. The traditional Chinese medicine composition consists of the following raw medicinal materials which have better treatment effect within the following dosage range in parts by weight: 100-300 parts of gallnuts, 50-150 parts of scutellaria baicalensis, 100-300 parts of folium isatidis, 100-300 parts of dandelions, 50-150 parts of pomegranate barks and 100-300 parts of humifuse euphorbia herb. The traditional Chinese medicine composition provided by the invention is a pure traditional Chinese medicine preparation, has a better inhibition effect on edwardsiella tarda disease of turbots, is non-toxic and free of pollution, does not generate drug resistance, and is low in price and worthy of development and utilization.
Description
Technical field
The present invention relates to a kind of Chinese medicine composition and preparation method thereof, particularly a kind of for suppressing the Chinese medicine composition of turbot Edwardsiella tarda disease, belong to fish immunity preparation preparing technical field.
Background technology
Edwardsiella tarda disease is one of maximum bacterial diseases of fishes of harm, its cause of disease Edwardsiella tarda (Edw ardsiella tarda) is a kind of Gram-negative enteric pathogenic bacteria, infection host scope is wide, in aquaculture organisms, this bacterium is the important pathogenic bacteria of Fish (eel, catfish, Flounder etc.), amphibian (frog), reptiles (Trionyx sinensis Wiegmann, crocodile), be one of modal infectious disease in aquaculture, affected the healthy of aquaculture organism huge to culture fishery harm.Turbot is the principal item of world today's aquaculture industry, has produced huge economic and social benefit, in recent years, because Edwardsiella tarda is sick popular, has caused huge economic loss to turbot aquaculture.
Domestic is mainly to rely on antibiotic to the treatment of Edwardsiella tarda disease, as sulfa drugs and tetracycline etc.Along with antibiotic a large amount of uses, the problem facing is now that drug-fast bacterial strain is more and more, but also has brought host for the problem of side effects of pharmaceutical drugs and the environmental pollution that causes, and therefore antibiotic effect is also more and more restricted.Because Edwardsiella tarda has more serotype, the antigenic structure of LPS is complicated, also do not have at present a kind of can be for the LPS vaccine of all different serotypes, economic and practical Edwardsiella tardas.The features such as Chinese herbal medicine has inexpensive, low toxicity, side effect is little, be difficult for developing immunity to drugs, in medicine, some composition not only has antibacterial action, and also has immunization, can improve the immune state of body, improves self resistance against diseases.
Summary of the invention
The object of this invention is to provide a kind of for suppressing the Chinese medicine composition of turbot Edwardsiella tarda disease, thereby make up the deficiencies in the prior art.
First the present invention provides a kind of Chinese medicine composition, the crude drug of this medicine is comprised of Galla Chinensis, Pericarpium Granati, Folium Isatidis, Herba Euphorbiae Humifusae, Radix Scutellariae, Folium Artemisiae Argyi and Herba Taraxaci, and its consumption has good curative effect in following ranges: Galla Chinensis 100～300 weight portions, Radix Scutellariae 50～150 weight portions, Folium Isatidis 100～300 weight portions, Herba Taraxaci 100～300 weight portions, Pericarpium Granati 50～150 weight portions, Herba Euphorbiae Humifusae 100～300 weight portions.
Preferred crude drug weight proportion is: Galla Chinensis 200 weight portions, Radix Scutellariae 100 weight portions, Folium Isatidis 200 weight portions, Herba Taraxaci 200 weight portions, Pericarpium Granati 100 weight portions, Herba Euphorbiae Humifusae 200 weight portions.
Chinese medicine composition of the present invention is prepared into preparation, and preparation method is as follows:
Get Galla Chinensis, Radix Scutellariae, Folium Isatidis, Herba Taraxaci, Pericarpium Granati, Herba Euphorbiae Humifusae, add 8～12 times of water gagings and decoct 2～4 times, add at every turn and decoct 0.5～1.5 hour, filter, merging filtrate, is concentrated into the concentrated solution that solid-liquid ratio is 1:1～2, obtains.
Further preferred preparation technology is:
Get Galla Chinensis, Radix Scutellariae, Folium Isatidis, Herba Taraxaci, Pericarpium Granati, Herba Euphorbiae Humifusae, add 10 times of water gagings and decoct 3 times, add at every turn and decoct 1.0 hours, filter, merging filtrate, is concentrated into the concentrated solution that solid-liquid ratio is 1:1, obtains.
More than form according to weight portion as proportioning, when producing, can increase or reduce according to corresponding ratio, as large-scale production can be with a kilogram Huo Yidunwei unit, small-scale production also can be in grams, weight can increase or reduce, but each raw medicinal herbs weight proportion constant rate between forming.
Chinese medicine composition of the present invention can be applied in the medicine that suppresses turbot Edwardsiella tarda disease.
The present invention is pure Chinese medicinal preparation, is the Edwardsiella tarda disease of turbot is had to good inhibition, and nontoxic, pollution-free, do not produce drug resistance, cheap, be worth developing.
The specific embodiment
Galla Chinensis function used in the present invention astringe the lung pathogenic fire reducing, relieving diarrhea with astringents, astringing to arrest bleeding, removing dampness sore, cure mainly fish rotten cheek, Rhizoma Euonymus, red skin, furuncle etc.Radix Scutellariae function heat clearing and damp drying, eliminating fire and detoxication, hemostasis, cure mainly fish gill rot, red skin, enteritis, hemorrhage etc.Folium Isatidis function heat-clearing and toxic substances removing, blood cooling and ecchymoses removing, cure mainly calentura and send out speckle, carbuncle, erysipelas etc.Herba Taraxaci function heat-clearing and toxic substances removing, dispersing swelling and dissipating binds, inducing diuresis for treating stranguria syndrome, cure mainly sore, conjunctival congestion etc.Pericarpium Granati function relieving diarrhea with astringents, hemostasis, parasite killing, cure mainly dysentery, have blood in stool, proctoptosis, malnutrition due to parasitic infestation etc.Herba Euphorbiae Humifusae function heat-clearing and toxic substances removing, cooling blood for hemostasis, cure mainly fish enteritis, gill rot, carbuncle malignant boil etc.And its consumption is as follows: Galla Chinensis 100～300 weight portions, Radix Scutellariae 50～150 weight portions, Folium Isatidis 100～300 weight portions, Herba Taraxaci 100～300 weight portions, Pericarpium Granati 50～150 weight portions, Herba Euphorbiae Humifusae 100～300 weight portions.Above-mentioned component is proportioning, guarantees that turbot Edwardsiella tarda disease is had to prevention, therapeutic effect on the one hand; And can also guarantee on the other hand can not affect during use the picked-up of turbot to bait can not reduce growth rate.
Enumerate embodiment below, further illustrate the present invention, each embodiment only, for the present invention is described, does not limit the present invention:
Embodiment 1
Galla Chinensis 200g Radix Scutellariae 100g Folium Isatidis 200g Herba Taraxaci 200g Pericarpium Granati 100g Herba Euphorbiae Humifusae 200g
Get Galla Chinensis, Radix Scutellariae, Folium Isatidis, Herba Taraxaci, Pericarpium Granati, Herba Euphorbiae Humifusae, add 10 times of water gagings and decoct 3 times, add at every turn and decoct 1.0 hours, filter, merging filtrate, is concentrated into 1000ml, obtains.
Embodiment 2
Galla Chinensis 300g Radix Scutellariae 150g Folium Isatidis 200g Herba Taraxaci 150g Pericarpium Granati 100g Herba Euphorbiae Humifusae 100g
Get Galla Chinensis, Radix Scutellariae, Folium Isatidis, Herba Taraxaci, Pericarpium Granati, Herba Euphorbiae Humifusae, add 12 times of water gagings and decoct 2 times, add at every turn and decoct 1.5 hours, filter, merging filtrate, is concentrated into 2000ml, obtains.
Embodiment 3
Galla Chinensis 100g Radix Scutellariae 50g Folium Isatidis 300g Herba Taraxaci 100g Pericarpium Granati 150g Herba Euphorbiae Humifusae 300g
Get Galla Chinensis, Radix Scutellariae, Folium Isatidis, Herba Taraxaci, Pericarpium Granati, Herba Euphorbiae Humifusae, add 8 times of water gagings and decoct 4 times, add at every turn and decoct 0.5 hour, filter, merging filtrate, is concentrated into 1000ml, obtains.
Embodiment 4
Galla Chinensis 200g Radix Scutellariae 150g Folium Isatidis 100g Herba Taraxaci 300g Pericarpium Granati 50g Herba Euphorbiae Humifusae 200g
Get Galla Chinensis, Radix Scutellariae, Folium Isatidis, Herba Taraxaci, Pericarpium Granati, Herba Euphorbiae Humifusae, add 10 times of water gagings and decoct 3 times, add at every turn and decoct 1.0 hours, filter, merging filtrate, is concentrated into 1000ml, obtains.For further verifying the therapeutical effect of Chinese medicine composition of the present invention, the inhibition test of Edwardsiella tarda disease is investigated:
1. the extracorporeal bacteria inhibitor test of pair Edwardsiella tarda
(1) antibacterial culturing Edwardsiella tarda inoculation, in nutrient broth fluid medium, is put 37 ℃ and is cultivated after 24h, with colony counting method, determines bacterial concentration, and with physiological saline solution dilution, obtaining final concentration is 6.0 * 10
7the bacterium liquid of cfu/mL.
(2) inhibition zone is measured the about 12.00cm of nutrient agar plate diameter, the about 7.00mm of culture medium thickness, on each flat board, add 200 μ L bacterium liquid, smear evenly, then use aseptic card punch (diameter is 6.00mm) to punch on each flat board, carry out drug labelling, finally 100 μ L are added in hand-hole for reagent liquid.Cultivate 24 hours for 35 ℃, measure antibacterial circle diameter.
(3) test results test the results are shown in Table 1.
Table 1: the extracorporeal bacteria inhibitor test result table (n=8) of the present invention to Edwardsiella tarda
Note: test liquid concentration is obtained through dilution by the 1g/ml solution of embodiment 1 gained.
Above experimental result shows, this patent medicine has certain inhibition to Edwardsiella tarda under low consistency conditions, and the higher effect of concentration is more obvious, illustrates that this patent medicine can be used for treating Edwardsiella tarda disease.
2. the sick clinical therapeutic efficacy of the Edwardsiella tarda of pair turbot
(1) experimental technique is got test fish 200 tails that infect Edwardsiella tarda disease, is equally divided at random 2 groups, and every group of 100 tails are divided into blank group and supply examination group (10mg crude drug/ml).Blank group is put in normal water and is treated, and for examination group, put in test liquid and treat, once a day, continuous 20 days.
(2) observational technique Continuous Observation 20 days from dispensing, respectively observes in every morning, afternoon 1 time, carries out in time observed and recorded.
(3) during the Control experiment of breeding environment, feed and carry out according to a conventional method with aquaculture management, be consistent as far as possible.
(4) criterion is cured: test fish is fully recovered gradually, and do not recur; Effective: test fish symptom has clear improvement, or recovery from illness but recurrence gradually; Invalid: after medication, test fish symptom is not alleviated, even increase the weight of dead.For statistical facility, in not administration group, depending on its remission situation, also according to above-mentioned standard, add up classification.
(5) experimental result experimental result is in Table 2.
Table 2: to the sick clinical therapeutic efficacy experimental result of the Edwardsiella tarda of turbot table
Note: test liquid concentration is obtained through dilution by 1g crude drug/ml solution of embodiment 1 gained.
Above experimental result shows, this Chinese medicine test liquid has obvious therapeutic effect to the Edwardsiella tarda disease of turbot, cure rate 68%, total effective rate 92%.Illustrate that this patent group has clinical treatment effect to the Edwardsiella tarda disease of turbot.
And, the statistical result of the rate of growth of turbot is shown, what Chinese medicine composition of the present invention was used does not have the difference on statistical significance for examination group and the growth rate of the turbot of not administration group.Therefore,, when Chinese medicine composition of the present invention carrys out prophylactic treatment Edwardsiella tarda in raising turbot immunity, can not affect the feeding rate of turbot; Than existing chemical agent, there is better effect.
Claims (6)
1. a Chinese medicine composition, is characterized in that, described Chinese medicine composition includes following component: Galla Chinensis 100～300 weight portions; Radix Scutellariae 50～150 weight portions; Folium Isatidis 100～300 weight portions; Herba Taraxaci 100～300 weight portions; Pericarpium Granati 50～150 weight portions; Herba Euphorbiae Humifusae 100～300 weight portions.
2. Chinese medicine composition as claimed in claim 1, is characterized in that, described Chinese medicine composition includes following component: Galla Chinensis 200 weight portions; Radix Scutellariae 100 weight portions; Folium Isatidis 200 weight portions; Herba Taraxaci 200 weight portions; Pericarpium Granati 100 weight portions; Herba Euphorbiae Humifusae 200 weight portions.
3. Chinese medicine composition as claimed in claim 1, is characterized in that, described Chinese medicine composition is comprised of following component: Galla Chinensis 100～300 weight portions; Radix Scutellariae 50～150 weight portions; Folium Isatidis 100～300 weight portions; Herba Taraxaci 100～300 weight portions; Pericarpium Granati 50～150 weight portions; Herba Euphorbiae Humifusae 100～300 weight portions.
4. the application of the Chinese medicine composition described in claim 1-3 any one in the sick treatment of preparation turbot Edwardsiella tarda preparation.
5. a preparation that is used for the treatment of turbot Edwardsiella tarda disease, it is characterized in that, described preparation is the Galla Chinensis of Chinese medicine composition claimed in claim 1, Radix Scutellariae, Folium Isatidis, Herba Taraxaci, Pericarpium Granati, Herba Euphorbiae Humifusae to be added to 8～12 times of water gagings decoct 2～4 times, add at every turn and decoct 0.5～1.5 hour, filter, merging filtrate, the concentrated solution that is concentrated into solid-liquid ratio and is 1:1～2 makes.
6. preparation as claimed in claim 5, it is characterized in that, described preparation is by the Galla Chinensis of Chinese medicine composition claimed in claim 1, Radix Scutellariae, Folium Isatidis, Herba Taraxaci, Pericarpium Granati, Herba Euphorbiae Humifusae, adding 10 times of water gagings decocts 3 times, add at every turn and decoct 1.0 hours, filter, merging filtrate, the concentrated solution that is concentrated into solid-liquid ratio and is 1:1 makes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410181491.4A CN103919929A (en) | 2014-04-30 | 2014-04-30 | Traditional Chinese medicine composition used for inhibiting turbot edwardsiella tarda disease |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410181491.4A CN103919929A (en) | 2014-04-30 | 2014-04-30 | Traditional Chinese medicine composition used for inhibiting turbot edwardsiella tarda disease |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103919929A true CN103919929A (en) | 2014-07-16 |
Family
ID=51138471
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410181491.4A Pending CN103919929A (en) | 2014-04-30 | 2014-04-30 | Traditional Chinese medicine composition used for inhibiting turbot edwardsiella tarda disease |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103919929A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105250387A (en) * | 2015-11-04 | 2016-01-20 | 暨南大学 | Antibacterial Chinese herbal medicine compound for fishes as well as preparation method and application of antibacterial Chinese herbal medicine compound |
| CN106747714A (en) * | 2016-11-30 | 2017-05-31 | 都安瑶族自治县技术开发中心 | Improve the liquid fertilizer of strawberry nutrition |
| CN107232095A (en) * | 2017-06-05 | 2017-10-10 | 舟山市普陀兴海养殖优质种苗选育研究所 | A kind of method for artificially breeding of the sick improved seeds of the anti-tarda of lefteye flounder |
| CN108783115A (en) * | 2018-07-12 | 2018-11-13 | 漳州傲农牧业科技有限公司 | A kind of immunopotentiator and the preparation method and application thereof preventing red head disease of Pseudobagrus fulvidraco |
-
2014
- 2014-04-30 CN CN201410181491.4A patent/CN103919929A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105250387A (en) * | 2015-11-04 | 2016-01-20 | 暨南大学 | Antibacterial Chinese herbal medicine compound for fishes as well as preparation method and application of antibacterial Chinese herbal medicine compound |
| CN105250387B (en) * | 2015-11-04 | 2019-07-19 | 暨南大学 | A kind of fish antibacterial Chinese herbal medicines compound and its preparation method and application |
| CN106747714A (en) * | 2016-11-30 | 2017-05-31 | 都安瑶族自治县技术开发中心 | Improve the liquid fertilizer of strawberry nutrition |
| CN107232095A (en) * | 2017-06-05 | 2017-10-10 | 舟山市普陀兴海养殖优质种苗选育研究所 | A kind of method for artificially breeding of the sick improved seeds of the anti-tarda of lefteye flounder |
| CN108783115A (en) * | 2018-07-12 | 2018-11-13 | 漳州傲农牧业科技有限公司 | A kind of immunopotentiator and the preparation method and application thereof preventing red head disease of Pseudobagrus fulvidraco |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102908549B (en) | Preparation method of Chinese herbal medicine active fermentation health care liquid for preventing and treating bird flu | |
| CN103844143B (en) | Tilapia mossambica prevention streptococcosis compound Chinese herbal medicinal feed additive | |
| CN1241608C (en) | Antiviral, antibacterial and antiinflammatory herbal injection for treating livestock and fowl infectious disease | |
| CN101214311B (en) | Compound scutellaria injection for treating fowls and domestic animals infectious disease and preparation thereof | |
| CN103860715B (en) | A kind of compound Chinese medicine extract and application thereof preventing and treating coccidiosis of domestic fowls | |
| CN103919929A (en) | Traditional Chinese medicine composition used for inhibiting turbot edwardsiella tarda disease | |
| CN101209307A (en) | Chinese medicinal oral liquid with heat-clearing and detoxication, dampness-eliminating and dysentery-stopping efficacy | |
| CN101209297A (en) | Compound Chinese medicinal granule for treating chicken coccidiosis | |
| CN103341114A (en) | Application of amomum tsao-ko oil in preparation of drugs for treating bacterial infectious diseases | |
| CN104274574B (en) | The Chinese herbal feed additive and its method of a kind of anti-chicken diarrhea | |
| CN103877229B (en) | A kind of compound Chinese medicine extract and application thereof preventing and treating an avian colibacillosis | |
| CN105998153B (en) | Ramulus Et Folium Pithecellobii Lucidi extract is preparing the application in anti-ESBLs-producing bacteria Escherichia coli drug | |
| CN103372075A (en) | Traditional Chinese medicine superfine powder composition resistant to chicken colibacillosis and preparation method thereof | |
| CN103860627A (en) | Pithecellobium clypearia antibiosis veterinary drug | |
| CN106039138A (en) | Lactic acid bacteria traditional Chinese medicine fermentation antiviral preparation for aquaculture | |
| CN101411752A (en) | Granular formulation for treating children's pneumonia | |
| CN109432287B (en) | Traditional Chinese medicine oral liquid for preventing and treating excessive heat syndrome of poultry qi system and preparation method thereof | |
| CN106344523A (en) | Preparation method and application of polydatin phospholipid complex granule | |
| CN102973730B (en) | Chinese medicament for animals with functions of clearing heat and removing toxicity, resisting virus and improving body immunity and preparation method thereof | |
| CN105687315A (en) | Traditional Chinese medicine perfusion agent preventing and treating dairy cow mastitis and preparation method thereof | |
| CN101366852B (en) | Chinese medicinal herb for treating riemerella anatipestifer disease and preparation method thereof | |
| CN103919889B (en) | A kind of Chinese medicine composition strengthening turbot immunity | |
| CN113694096B (en) | Licorice extract and preparation method and application thereof | |
| CN102078529B (en) | Chinese medicinal preparation for treating common cold in children | |
| CN108434369A (en) | Treat the probiotics herbal mixture zymotic fluid of chicken colibacillosis |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20140716 |