CN105250387B - A kind of fish antibacterial Chinese herbal medicines compound and its preparation method and application - Google Patents
A kind of fish antibacterial Chinese herbal medicines compound and its preparation method and application Download PDFInfo
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Abstract
The present invention discloses a kind of fish antibacterial Chinese herbal medicines compound and its preparation method and application.The Chinese herb compound is prepared by following bulk pharmaceutical chemicals by weight percentage: Chinese gall 10~20%, FRUCTUS TERMINALIAE IMMATURUS 20~40%, granatum 10~30%, Caulis Spatholobi 8~20%, water banyan 6~20%.Bulk pharmaceutical chemicals combination of the present invention is compact, and production method is easy, and drug cost is not high.Compound of the invention, which can effectively press down, kills a variety of aquatic pathogenic bacteriums, streptococcus effect is especially good, it is the safe green product of ideal prevention and treatment fish bacteria disease for oral administration especially Tilapia mossambica streptococcosis, the product is degradable in environment and fish body, noresidue, it can ensure water environment and fish quality, while induction pathogen being avoided to generate antibody-resistant bacterium.
Description
Technical field
The invention belongs to aquaculture field, in particular to a kind of fish antibacterial (especially streptococcus) Chinese herb compound and
Preparation method and application.
Background technique
Bacterial disease is the important diseases of cultured fishes, wherein streptococcus causes Tilapia mossambica mortality, causes tremendous economic
Loss, prevention and treatment fish bacteria sick (including Tilapia mossambica streptococcosis) are to ensure that fish farming industry sound development is essential to arrange
It applies.The various bacterial diseases including streptococcosis mainly are prevented and treated using chemicals all the time, however frequently using chemistry
Not only inducible resistance pathogenic strains generate drug, but also medicament residue can also cause fish quality problem.Chinese herbal medicine is low
Poison low-residual, Small side effects, is not easy that pathogen is induced to develop drug resistance, and both contains antimicrobial component, can directly sterilize, again
The substance for having enhancing immune, can improve the immune state of body, improve itself disease resistance, have exploitation at highly effective and safe green
The prospect of fishing medicine solves the problems, such as antibody-resistant bacterium generation and chemicals residue during the various bacterial diseases such as prevention and treatment streptococcus.
Summary of the invention
In order to overcome the disadvantages and deficiencies of the prior art, the primary purpose of the present invention is that providing a kind of fish with antibacterial medium-height grass
Medicine compound.The directly anti-aquatic pathogenic bacterium of the Chinese herb compound especially streptococcus (Streptococcus), is particularly suitable for Rofe
The prevention and treatment of streptococcosis during fish culture.
Another object of the present invention is to provide a kind of preparation methods of above-mentioned fish antibacterial Chinese herbal medicines compound.
A further object of the present invention is to provide a kind of applications of above-mentioned fish antibacterial Chinese herbal medicines compound.
The purpose of the invention is achieved by the following technical solution:
A kind of fish antibacterial Chinese herbal medicines compound, the Chinese herb compound by it is following by weight percentage bulk pharmaceutical chemicals preparation and
At: Chinese gall (Galla chinensis) 10~20%, FRUCTUS TERMINALIAE IMMATURUS (Terminalia chebula) 20~40%, granatum
(Punica granatum) 10~30%, Caulis Spatholobi (Spatholobi caullis) 8~20%, water banyan
(Cleistocalyx operculatus) 6~20%.
The preparation method of fish antibacterial Chinese herbal medicines compound, including following operating procedure:
(1) it by bulk pharmaceutical chemicals Chinese gall, FRUCTUS TERMINALIAE IMMATURUS, granatum, Caulis Spatholobi and water banyan, is crushed after being separately dried, is sieved, obtains
To each bulk pharmaceutical chemicals dry powder；
(2) each bulk pharmaceutical chemicals dry powder described in step (1) is mixed according to above-mentioned weight percent, obtains fish with antibacterial medium-height grass
Medicine compound dry powder.
Drying described in step (1) is to dry in an oven in 55~65 DEG C；
The mesh number of sieving described in step (1) is 200~300 mesh.
Step (2) resulting fish uses plastic bag sealing with antibacterial Chinese herbal medicines compound dry powder, is placed at dry and stores.
A kind of preparation method of above-mentioned fish antibacterial Chinese herbal medicines compound can also include following operating procedure:
(1) it by bulk pharmaceutical chemicals Chinese gall, FRUCTUS TERMINALIAE IMMATURUS, granatum, Caulis Spatholobi and water banyan, is crushed after being separately dried, is sieved, obtains
To each bulk pharmaceutical chemicals dry powder；
(2) each bulk pharmaceutical chemicals dry powder obtained by step (1) is mixed by above-mentioned weight percent；It is continuous to decoct after soaking in water
Twice, it filters, merging filtrate obtains filtrate A；
(3) filtrate A obtained by step (2) is boiled, is concentrated, obtains fish antibacterial Chinese herbal medicines compound liquid preparation.
Drying described in step (1) is 55~65 DEG C of drying in an oven；
The mesh number of sieving described in step (1) is 40~60 mesh.
Described in step (2) plus the volume of water is 15~30 times of the total weight after each bulk pharmaceutical chemicals dry powder blend；
The temperature of immersion described in step (2) is 60~80 DEG C, and soaking time is 60 minutes~120 minutes；
It is to use to be cooked by slow fire 50 minutes~60 minutes that the first time of decoction twice described in step (2), which decocts, second
Decoction is to use to be cooked by slow fire 40 minutes~45 minutes；
Filtering described in step (2) is first then to use silk cover filtering with after three layers of filtered through gauze.
Fish described in step (3) is prepared 2 according to 500g bulk pharmaceutical chemicals with the concentration of antibacterial Chinese herbal medicines compound liquid preparation
Fish is risen to be calculated with antibacterial Chinese herbal medicines compound medical fluid.
A kind of application of the above-mentioned fish with antibacterial Chinese herbal medicines compound in the feed addictive or feed of preparation fish antibacterial.
The fish are preferably Tilapia mossambica；
The antibacterial is preferably antibacterium；
The antibacterium is preferably streptococcus.
With antibacterial Chinese herbal medicines compound, whether dry powder or liquid preparation all have obvious anti-aquatic pathogenic bacterium to fish of the present invention
Especially streptococcus acts on.By fish antibacterial Chinese herbal medicines compound dry powder or liquid preparation according to mass percentage 2 when use
~4% ratio, which is added in feed, feeds fish, and feeding volume is the 3~5% of fish body weight.
The principle of the present invention is: each Chinese herbal medicine in the compound all has antibacterial functions, such as Streptococcusagalactiae
(streptococcus agalactiae), Streptococcus iniae (Streptococcus iniae), Aeromonas hydrophila
(Aeromonas hydrophila), Aeromonas sobria (Aeromonas sobria) etc..Chinese gall is not because containing tannin, eating
Plasm and enzyme in the component damages such as sub- acid and Penta-O-galloyl-D-glucopyranose bacterial body and play antibacterial action；FRUCTUS TERMINALIAE IMMATURUS is to scold
Son drying young fruit, containing tannin class, galloyl glucose class, the simple ester type compound of nutgall acyl, gallic acid and
The compounds such as its ethyl ester have significant antibacterial action；Tannin, flavone compound in granatum are the active components of its antibacterial；
Caulis Spatholobi contains the effective components such as osajin (such as onocerin, ononin, cajinin) and calycosin, can inhibit big
Enterobacteria (Escherichia coli) and staphylococcus aureus (Staphylococcus aureus)；Water banyan, which contains, to be waved
Hair oil inhibits bacterial growth.Mentioned component is in addition to having an antibacterial effect, moreover it is possible to enhance immunization, and have association between each other
Same antibacterial action.
The present invention compared with the existing technology, have following advantages and effects
(1) fish of the present invention antibacterial Chinese herbal medicines compound has the work of significant anti-aquatic pathogenic bacterium especially streptococcal infection fish
With, will not inducible resistance bacterial strain generate, drug degradable noresidue in water body and fish body avoids antibiotics residues and makes
At fish quality risk and inducible resistance bacterial strain generate medicine risk.
(2) bulk pharmaceutical chemicals combination of the present invention is compact, and production method is easy, and drug cost is not high, is suitble to oneself make by oneself with prescription
Medicament.
(3) compound, which can effectively press down, kills a variety of aquatic pathogenic bacteriums, and streptococcus effect is especially good, is ideal prevention and treatment for oral administration
The safe green product of fish bacteria disease especially Tilapia mossambica streptococcosis, the product is degradable in environment and fish body, without residual
It stays, can ensure water environment and fish quality, while induction pathogen being avoided to generate antibody-resistant bacterium.
Specific embodiment
Below with reference to embodiment, the present invention is described in further detail, and embodiments of the present invention are not limited thereto.
In document, " 100 kinds of Chinese herbal medicine water extracts press down in vitro to kill Streptococcusagalactiae (streptococcus agalactiae)
Drug efficacy study [J] ecological science of Streptococcusagalactiae discloses in 2015,03:26-30. "；
In document, " double PCR quickly identifies Streptococcusagalactiae and dolphin to Streptococcus iniae (Streptococcus iniae)
Streptococcus [J] Agricultural University Of Hunan's journal (natural science edition) discloses in 2010,04:449-452. "；
Aeromonas hydrophila (Aeromonas hydrophila) is in document " body of 42 kinds of Chinese herbal medicines to Aeromonas hydrophila
Outer bacteriostatic test [J] fresh water fishery discloses in 2011,03:61-65. "；
Aeromonas sobria (Aeromonas sobria) in document, " exempt from by the immune Spinibarbus sinensis peripheral blood of inactivated vaccine
Variation [J] aquatic product journal of epidemic disease index discloses in 2010,04:626-634. "；
Vibrio vulnificus (Vibrio vulnificus) is in document " the separation identification and drug sensitive test of Tilapia mossambica Vibrio vulnificus
[J] Agricultural University Of Jiangxi journal discloses in 2011,05:965-970. "；
Edwardsiella tarda (Edwardsiella tarda) is in the document " building of eel source Edwardsiella tarda ghost
And preparation condition optimizes [J] aquatic product journal, discloses in 2012,11:1754-1762. "；
Aeromonas veronii (Aeromonas veronii) document " Tilapia mossambica Aeromonas veronii separation identification and
Drug sensitive test [J] Ecology magazine discloses in 2011,03:132-136. ".
Embodiment 1
(1) bulk pharmaceutical chemicals are crushed after 55 DEG C of drying in an oven respectively, crosses 40 meshes, obtains each bulk pharmaceutical chemicals dry powder；
(2) each bulk pharmaceutical chemicals dry powder obtained by step (1) is uniformly mixed by following weight: Chinese gall 70g, FRUCTUS TERMINALIAE IMMATURUS 180g,
Granatum 150g, Caulis Spatholobi 50g, water banyan 50g, then plus 7.5 liters of 60 DEG C of water impregnate, and keep 60 DEG C of 2h, be then heated to boiling
It rises, then is cooked by slow fire 50 minutes, after 3 layers of filtered through gauze, then with silk cover filtering, the dregs of a decoction add 7.5 liters of water to be heated to boiling again, then
It is cooked by slow fire 45 minutes, is filtered with preceding method, merge filtrate twice, obtain filtrate A；
(3) filtrate A is boiled, is concentrated into 2 liters, be fish antibacterial Chinese herbal medicines compound liquid preparation.
The Chinese gall of the present embodiment originates from Sichuan, and FRUCTUS TERMINALIAE IMMATURUS originates from Yunnan, and granatum originates from Shandong, and Caulis Spatholobi originates from extensively
East, water banyan originate from Guangxi, and the Chinese herbal medicine place of production of following embodiment is identical with this.
Embodiment 2
(1) bulk pharmaceutical chemicals are crushed after 60 DEG C of drying in an oven respectively, crosses 50 meshes, obtains each bulk pharmaceutical chemicals dry powder；
(2) each bulk pharmaceutical chemicals dry powder obtained by step (1) is uniformly mixed by following weight: Chinese gall 100g, FRUCTUS TERMINALIAE IMMATURUS 100g,
Granatum 150g, Caulis Spatholobi 75g, water banyan 75g are put into 15 liters of 70 DEG C of water and impregnate, and are kept for 70 DEG C 100 minutes, then heated
It to boiling, then is cooked by slow fire 55 minutes, after 3 layers of filtered through gauze, then with silk cover filtering, the dregs of a decoction add 15 liters of water to be heated to boiling again
It rises, then is cooked by slow fire 40 minutes, filtered with preceding method, merge filtrate twice, obtain filtrate A；
(3) filtrate A is boiled, is concentrated into 2 liters, be fish antibacterial Chinese herbal medicines compound liquid preparation.
Embodiment 3
(1) bulk pharmaceutical chemicals are crushed after 65 DEG C of drying in an oven respectively, crosses 60 meshes, obtains each bulk pharmaceutical chemicals dry powder；
(2) each bulk pharmaceutical chemicals dry powder obtained by step (1) is uniformly mixed by following weight: Chinese gall 80g, FRUCTUS TERMINALIAE IMMATURUS 200g,
Granatum 150g, Caulis Spatholobi 40g, water banyan 30g are put into 10 liters of 80 DEG C of water and impregnate, and are kept for 80 DEG C 60 minutes, are then heated to
Boiling, then is cooked by slow fire 50 minutes, and after 3 layers of filtered through gauze, then with silk cover filtering, the dregs of a decoction add 10 liters of water to be heated to boiling again,
It is cooked by slow fire 45 minutes, is filtered with preceding method again, merge filtrate twice, obtain filtrate A；
(3) filtrate A is boiled, is concentrated into 2 liters, be fish antibacterial Chinese herbal medicines compound liquid preparation.
Embodiment 4
(1) bulk pharmaceutical chemicals are crushed after 55 DEG C of drying in an oven respectively, crosses 200 meshes, obtains each bulk pharmaceutical chemicals dry powder；
(2) each bulk pharmaceutical chemicals dry powder obtained by step (1) is uniformly mixed by following weight: Chinese gall 50g, FRUCTUS TERMINALIAE IMMATURUS 200g,
Granatum 50g, Caulis Spatholobi 100g, water banyan 100g obtain fish antibacterial Chinese herbal medicines compound dry powder；By the fish antibacterial Chinese herbal medicines
Compound dry powder polybag is placed at dry and is stored with every bag of sealed packaging of 0.5kg.
Embodiment 5
(1) bulk pharmaceutical chemicals are crushed after 60 DEG C of drying in an oven respectively, crosses 250 meshes, obtains each bulk pharmaceutical chemicals dry powder；
(2) each bulk pharmaceutical chemicals dry powder obtained by step (1) is uniformly mixed by following weight: Chinese gall 100g, FRUCTUS TERMINALIAE IMMATURUS 170g,
Granatum 100g, Caulis Spatholobi 70g, water banyan 60g obtain fish antibacterial Chinese herbal medicines compound Chinese herb dry powder；By the fish antibacterial
Chinese herb compound dry powder polybag is placed at dry and is stored with every bag of sealed packaging of 0.5kg.
Embodiment 6
(1) bulk pharmaceutical chemicals are crushed after 65 DEG C of drying in an oven respectively, crosses 300 meshes, obtains each bulk pharmaceutical chemicals dry powder；
(2) each bulk pharmaceutical chemicals dry powder obtained by step (1) is uniformly mixed by following weight: Chinese gall 70g, FRUCTUS TERMINALIAE IMMATURUS 185g,
Granatum 145g, Caulis Spatholobi 50g, water banyan 50g obtain fish antibacterial Chinese herbal medicines compound dry powder；By the fish antibacterial Chinese herbal medicines
Compound dry powder polybag is placed at dry and is stored with every bag of sealed packaging of 0.5kg.
Embodiment 7
Test method:
Fish prepared by embodiment 1 is used to anti-aquatic pathogenic bacterium with antibacterial Chinese herbal medicines compound liquid preparation to test, using solid
Body plate diffusion bacteriostatic method Preliminary detection compound liquid preparation can press down the aquatic products cause of disease killed, and further use two times of gradient dilutions
Method determines that the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of aquatic products cause of disease are killed in compound liquid preparation suppression.This experiment
The concrete operations of the solid plate diffusion bacteriostatic method used are uniformly coated on solid culture for the bacterium solution for taking 0.1mL with pipettor
On base, flowed to no liquid, with punch | (diameter 6mm) makes a call to four equally distributed holes on solid medium, wherein three
Hole adds compound liquid preparation (pH value is adjusted to 7.0 or so), another hole adds fluid nutrient medium (not dosing) as control, later,
Culture dish is placed in 28 DEG C of constant incubators and is cultivated for 24 hours, measurement record antibacterial circle diameter；The operation side of two times of gradient dilution methods
Method is with every kind of bacterium for one group, and every group takes 12 sterile test tubes and number, and is separately added into fluid nutrient medium 2mL in preceding 10 branch pipe,
Compound liquid preparation 2mL is added to No. 1 pipe with pipettor again, after mixing well, takes 2mL to be put into No. 2 pipes from No. 1 pipe and mixes,
And so on, 2mL is finally sucked out from No. 10 pipe and abandons, bacterium solution 0.5mL is respectively added in 10 branch pipe of face further along, finally to control
2mL fluid nutrient medium and 0.5mL bacterium solution is added in pipe 1, and 2.5mL fluid nutrient medium is added in control tube 2, is placed in 28 in constant-temperature table
DEG C concussion (180rmin-1) cultivate for 24 hours, each pipe culture solution 0.1mL is drawn respectively, is coated on corresponding agar plate, 28
For 24 hours, the corresponding drug concentration of drug highest dilution tube of no bacterial growth is this kind of drug to the minimum for trying bacterium for DEG C culture
Mlc (MIC).MIC is managed and a concentration (higher concentration) pipe continues culture for 24 hours thereon, then draws the two concentration pipe respectively
Interior culture solution 0.1mL, is coated on corresponding agar plate, is further cultured for for 24 hours in 28 DEG C, corresponding to the agar plate of asepsis growth
The concentration of medical fluid dilution tube be minimum bactericidal concentration (MBC).
Experimental result: Chinese herb compound can effectively inhibit a variety of aquatic pathogenic bacterium (tables including Streptococcusagalactiae
1)。
1 Chinese herb compound of table kills effect to the suppression of 7 kinds of aquatic pathogenic bacteriums
Embodiment 8
Test method:
(1) fish for preparing embodiment 3 is sprayed with antibacterial Chinese herbal medicines compound liquid preparation according to the ratio of feeding quality 4%
Pot is uniformly sprayed on floating pellet diet, is placed on 55 DEG C of drying box drying, is obtained Chinese herbal feed, spare.
(2) 32.54 ± 2.23 grams of weight of Tilapia mossambica (Oreochromis spp.) is used in experiment, is purchased from Guangdong Tilapia mossambica breeding
, temporarily domesticating 2 weeks before testing, inflator pump oxygen supply feeds common fish feed in 9 points of every morning and at 5 points in afternoon.By Tilapia mossambica
It is divided into 3 groups, every group 3 parallel repetitions are tested, respectively 3 repetitions of experimental group, 2 each 3 repetitions of control group 1 and control group, often
A 30 tail fish of repetition, experimental group and control group 1 are respectively with 5 × 106cfu·mL-1Streptococcusagalactiae intraperitoneal injection Tilapia mossambica it is every
Tail 0.2mL, control group 2 inject equivalent 0.65% physiological saline of sterilizing.In experimental group, after injection bacterium for 24 hours, according to fish body weight
3% feeds the Chinese herbal feed prepared in step (1), and control group feeds the normal diet that fish body weighs 3%, daily early 9 points and evening 5
Point is respectively fed 1 time.The activity condition of Tilapia mossambica is observed, dead fish time and quantity are recorded, until test group fish is completely healthy or completely dead
Until dying, survival rate is counted.During test, dead fish is cleared up in time.
Test result: the control group 1 for having injected Streptococcusagalactiae is completely dead, survival rate 0, pair of injecting normal saline
According to complete survival, survival rate 100% feeds 3 repetitions of test group of fish antibacterial Chinese herbal medicines compound liquid preparation feed respectively
Survive 28 tails, 27 tails and 25 tail fishes, survival rate average out to 88.9%.
Embodiment 9
Test method:
(1) base is added in the ratio of mass percentage 2% with antibacterial Chinese herbal medicines compound dry powder in the fish for preparing embodiment 4
In plinth buoyancy feed, it is machined to floating pellet diet with bait, it is spare.
(2) test uses 35.63 grams of Tilapia mossambica average weight, temporarily domesticating 2 weeks before testing, inflator pump oxygen supply, and every morning 9
Point and at 5 points in afternoon feed normal diet.Tilapia mossambica is divided into 3 groups, every group 3 parallel repetitions are tested, respectively 3 weights of test group
Multiple, 2 each 3 repetitions of control group 1 and control group, each 30 tail fish of repetition, test group and control group 1 are respectively with 5 × 106cfu·
mL-1Streptococcusagalactiae be injected intraperitoneally the every tail 0.2mL of Tilapia mossambica, control group 2 inject equivalent sterilize 0.65% physiological saline.Test
In group, after injection bacterium for 24 hours, the floating pellet diet (Chinese herbal feed) prepared in step (1) is fed according to the 5% of fish body weight,
Control group feeds the normal diet that fish body weighs 5%, respectively feeds 1 time for early 9 points and late 5 points daily.Observe the activity condition of Tilapia mossambica, note
Record dead fish time and quantity, to test group fish it is completely healthy or it is complete it is dead until, count survival rate.It is clear in time during test
Manage dead fish.
Test result: the control group 1 for having injected Streptococcusagalactiae is completely dead, survival rate 0, pair of injecting normal saline
It survives completely according to group fish, survival rate 100% feeds 3 repetitions of test group of fish antibacterial Chinese herbal medicines compound dry powder feed respectively
Survive 29 tails, 26 tails and 26 tail fishes, survival rate average out to 90.0%.
Embodiment 10
Test method:
(1) fish for preparing embodiment 2 is sprayed with antibacterial Chinese herbal medicines compound liquid preparation according to the ratio of feeding quality 4%
Pot is uniformly sprayed on floating pellet diet, is placed on 55 DEG C of drying box drying, is obtained Chinese herbal feed, spare.
(2) experiment uses 36.38 ± 3.42 grams of Tilapia mossambica weight, temporarily domesticating 2 weeks before testing, inflator pump oxygen supply, and every the sky
9 points of noon and at 5 points in afternoon feed common fish feed.Tilapia mossambica is divided into 3 groups, every group 3 parallel repetitions are tested, and are respectively tested
3 repetitions of group, 2 each 3 repetitions of control group 1 and control group, each 30 tail fish of repetition, experimental group and control group 1 respectively with 5 ×
106cfu·mL-1Streptococcus iniae be injected intraperitoneally the every tail 0.2mL of Tilapia mossambica, control group 2 inject equivalent sterilize 0.65% physiology
Salt water.In experimental group, after injection bacterium for 24 hours, the Chinese herbal feed prepared in step (1), control group are fed according to the 3% of fish body weight
The normal diet that fish body weighs 3% is fed, is respectively fed 1 time for early 9 points and late 5 points daily.The activity condition of Tilapia mossambica is observed, dead fish is recorded
Time and quantity, to test group fish it is completely healthy or it is complete it is dead until, count survival rate.During test, dead fish is cleared up in time.
Test result: the control group 1 for having injected Streptococcus iniae is completely dead, survival rate 0, pair of injecting normal saline
According to complete survival, survival rate 100%, 3 repetitions of test group for feeding antibacterial Chinese herbal medicines compound preparation feed are survived 28 tails respectively,
25 tails and 25 tail fishes, survival rate average out to 86.6%.
Embodiment 11
(1) base is added in the ratio of mass percentage 2% with antibacterial Chinese herbal medicines compound dry powder in the fish for preparing embodiment 6
In plinth buoyancy feed, it is machined to floating pellet diet with bait, it is spare.
(2) test uses 38.26 ± 4.12 grams of Tilapia mossambica average weight, temporarily domesticating 2 weeks before testing, inflator pump oxygen supply, often
At 9 points in its morning and at 5 points in afternoon feed normal diet.Tilapia mossambica is divided into 3 groups, every group 3 parallel repetitions are tested, and are respectively tested
3 repetitions of group, 2 each 3 repetitions of control group 1 and control group, each 30 tail fish of repetition, test group and control group 1 respectively with 5 ×
106cfu·mL-1Streptococcus iniae be injected intraperitoneally the every tail 0.2mL of Tilapia mossambica, control group 2 inject equivalent sterilize 0.65% physiology
Salt water.In test group, after injection bacterium for 24 hours, the floating pellet diet (medium-height grass prepared in step (1) is fed according to the 5% of fish body weight
Medicine feed), control group feeds the normal diet that fish body weighs 5%, respectively feeds 1 time for early 9 points and late 5 points daily.Observe the work of Tilapia mossambica
Emotionally condition records dead fish time and quantity, to test group fish it is completely healthy or it is complete it is dead until, count survival rate.Experimental period
Between, dead fish is cleared up in time.
Test result: the control group 1 for having injected Streptococcus iniae is completely dead, survival rate 0, pair of injecting normal saline
It survives completely according to group fish, survival rate 100%, 3 repetitions of test group for feeding antibacterial Chinese herbal medicines compound preparation feed are survived respectively
27 tails, 29 tails and 25 tail fishes, average viability 90.0%.
The above embodiment is a preferred embodiment of the present invention, but embodiments of the present invention are not by above-described embodiment
Limitation, other any changes, modifications, substitutions, combinations, simplifications made without departing from the spirit and principles of the present invention,
It should be equivalent substitute mode, be included within the scope of the present invention.
Claims (10)
1. a kind of fish antibacterial Chinese herbal medicines composition, it is characterised in that: the herbal composite by it is following by weight percentage
Bulk pharmaceutical chemicals be prepared: Chinese gall 10~20%, FRUCTUS TERMINALIAE IMMATURUS 20~40%, granatum 10~30%, Caulis Spatholobi 8~20%,
Water banyan 6~20%.
2. the fish described in claim 1 preparation method of antibacterial Chinese herbal medicines composition, it is characterised in that walked including following operation
It is rapid:
(1) it by bulk pharmaceutical chemicals Chinese gall, FRUCTUS TERMINALIAE IMMATURUS, granatum, Caulis Spatholobi and water banyan, crushes, is sieved after being separately dried, obtain each
Bulk pharmaceutical chemicals dry powder；
(2) weight percent described in accordance with the claim 1 of each bulk pharmaceutical chemicals dry powder described in step (1) is mixed, obtains fish use
Antibacterial Chinese herbal medicines composition dry powder.
3. the fish according to claim 2 preparation method of antibacterial Chinese herbal medicines composition, it is characterised in that:
Drying described in step (1) is dried in 55~65 DEG C；
The mesh number of sieving described in step (1) is 200~300 mesh.
4. the fish according to claim 2 preparation method of antibacterial Chinese herbal medicines composition, it is characterised in that:
Step (2) resulting fish uses plastic bag sealing with antibacterial Chinese herbal medicines composition dry powder, is placed at dry and stores.
5. the fish described in claim 1 preparation method of antibacterial Chinese herbal medicines composition, it is characterised in that walked including following operation
It is rapid:
(1) it by bulk pharmaceutical chemicals Chinese gall, FRUCTUS TERMINALIAE IMMATURUS, granatum, Caulis Spatholobi and water banyan, crushes, is sieved after being separately dried, obtain each
Bulk pharmaceutical chemicals dry powder；
(2) each bulk pharmaceutical chemicals dry powder weight percent according to claim 1 obtained by step (1) is mixed；After soaking in water, even
Twice, filtering, merging filtrate obtains filtrate A for continuous decoction；
(3) filtrate A obtained by step (2) is boiled, is concentrated, obtains fish antibacterial Chinese herbal medicines composition liquid preparation.
6. the fish according to claim 5 preparation method of antibacterial Chinese herbal medicines composition, it is characterised in that:
Drying described in step (1) is dried at 55~65 DEG C；
The mesh number of sieving described in step (1) is 40~60 mesh.
7. the fish according to claim 5 preparation method of antibacterial Chinese herbal medicines composition, it is characterised in that:
Described in step (2) plus the volume of water is 15~30 times of the total weight after each bulk pharmaceutical chemicals dry powder blend.
8. the fish according to claim 5 preparation method of antibacterial Chinese herbal medicines composition, it is characterised in that:
The temperature of immersion described in step (2) is 60~80 DEG C, and the time is 60 minutes~120 minutes；
The first time of decoction twice described in step (2), which decocts, to be decocted for the second time using being cooked by slow fire 50 minutes~60 minutes
It is to use to be cooked by slow fire 40 minutes~45 minutes.
9. the fish according to claim 5 preparation method of antibacterial Chinese herbal medicines composition, it is characterised in that:
Fish described in step (3) is prepared 2 liters according to 500g bulk pharmaceutical chemicals with the concentration of antibacterial Chinese herbal medicines composition liquid preparation
Fish is calculated with antibacterial Chinese herbal medicines composition medical fluid.
10. fish described in claim 1 is with antibacterial Chinese herbal medicines composition in the feed addictive or feed of preparation fish antibacterial
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| CN110960635B (en) * | 2019-12-20 | 2021-07-27 | 暨南大学 | Chinese herbal medicine composition for preventing and treating monozoite disease for fish and preparation method and application thereof |
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| CN103125756A (en) * | 2011-11-29 | 2013-06-05 | 徐久春 | Traditional Chinese medicine feed additive for preventing fish disease |
| CN103919929A (en) * | 2014-04-30 | 2014-07-16 | 中国海洋大学 | Traditional Chinese medicine composition used for inhibiting turbot edwardsiella tarda disease |
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| CN103125756A (en) * | 2011-11-29 | 2013-06-05 | 徐久春 | Traditional Chinese medicine feed additive for preventing fish disease |
| CN103919929A (en) * | 2014-04-30 | 2014-07-16 | 中国海洋大学 | Traditional Chinese medicine composition used for inhibiting turbot edwardsiella tarda disease |
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