CN110960635B - Chinese herbal medicine composition for preventing and treating monozoite disease for fish and preparation method and application thereof - Google Patents
Chinese herbal medicine composition for preventing and treating monozoite disease for fish and preparation method and application thereof Download PDFInfo
- Publication number
- CN110960635B CN110960635B CN201911326795.4A CN201911326795A CN110960635B CN 110960635 B CN110960635 B CN 110960635B CN 201911326795 A CN201911326795 A CN 201911326795A CN 110960635 B CN110960635 B CN 110960635B
- Authority
- CN
- China
- Prior art keywords
- fish
- chinese herbal
- herbal medicine
- medicine composition
- treating
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/07—Basidiomycota, e.g. Cryptococcus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
- A61K36/237—Notopterygium
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/30—Boraginaceae (Borage family), e.g. comfrey, lungwort or forget-me-not
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/65—Paeoniaceae (Peony family), e.g. Chinese peony
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/71—Ranunculaceae (Buttercup family), e.g. larkspur, hepatica, hydrastis, columbine or goldenseal
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/899—Poaceae or Gramineae (Grass family), e.g. bamboo, corn or sugar cane
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P33/00—Antiparasitic agents
- A61P33/10—Anthelmintics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
Landscapes
- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Veterinary Medicine (AREA)
- Mycology (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Medicinal Chemistry (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- General Health & Medical Sciences (AREA)
- Alternative & Traditional Medicine (AREA)
- Epidemiology (AREA)
- Microbiology (AREA)
- Medical Informatics (AREA)
- Polymers & Plastics (AREA)
- Food Science & Technology (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Organic Chemistry (AREA)
- Insects & Arthropods (AREA)
- Marine Sciences & Fisheries (AREA)
- Birds (AREA)
- Medicines Containing Plant Substances (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Feed For Specific Animals (AREA)
- Fodder In General (AREA)
Abstract
The invention discloses a Chinese herbal medicine composition for preventing and treating monozoite diseases of fish and a preparation method and application thereof, belonging to the field of aquaculture. The Chinese herbal medicine composition is prepared from the following raw material medicines in percentage by weight: 5-10% of polyporus umbellatus, 25-40% of notopterygium root, 10-20% of lophatherum gracile, 15-25% of moutan bark and 10-20% of lithospermum. The raw material medicine resources of the invention are rich, the production method is simple and easy to implement, the medicine cost is low, the new bunyaworm disease can be effectively prevented and treated, and the invention is an ideal safe and green product for preventing and treating the fish monozoiasis, especially the new bunyaworm disease by oral administration; the Chinese herbal medicine composition enters the fish body in a feeding mode, has low toxicity to the fish, is easy to degrade in the environment, has no residue, ensures the water environment and the quality safety of aquatic products, and simultaneously reduces the consumption of manpower and material resources; is easy for industrialized production and popularization, and has wide application prospect in aquatic products.
Description
Technical Field
The invention belongs to the field of aquaculture, and particularly relates to a Chinese herbal medicine composition for preventing and treating monozoic diseases of fish, and a preparation method and application thereof.
Background
The new Nebrennentia spp is a kind of monozoites parasitizing on the body surface of marine fishes, belonging to the phylum Platyhelminthes, Monozoites, Chamber (Capsaladae) and Bennedenidae (Bennedeineae). The new Dendrolimus punctatus is widely distributed, has low specificity to hosts, and can parasitize more than 100 kinds of seawater fishes. The insect parasitizes on the body surface, fins, eyeballs, gill filaments and other parts of the fish, damages mucus layers and epithelial tissues of the fish, absorbs a large amount of host blood, swallows host epidermal cells, causes damage and ulceration of the fish surface, dyspnea, anemia, ulceration of the eyeballs and even blindness, often causes secondary bacterial infection and death, and causes huge loss to the mariculture industry. The main treatments used are fresh water and chemical insecticide treatments. However, the freshwater treatment consumes huge manpower and material resources, does not kill the ova and the larvae in the cage culture water area, and cannot radically cure the ova and the larvae; the pesticide has the problems of drug residue, large toxic and side effect, easy secondary pollution and the like. Therefore, the development of a safe and efficient product for preventing and treating the new benidiasis is urgently needed. The Chinese herbal medicine is a green natural medicine, has the advantages of small toxic and side effects, multiple components, multiple targets and multiple effects, easy degradation, no residue, no pollution and the like, can improve the disease resistance of the fish body after being taken orally, and has the prospect of developing a safe and efficient green fishing medicine.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention mainly aims to provide a Chinese herbal medicine composition for preventing and treating the monozoic diseases of the fish. The Chinese herbal medicine composition can obviously enhance the resistance of the fish body to the monozoite; is especially suitable for preventing and treating neokinsoniasis in the process of culturing marine fishes.
The invention also aims to provide a preparation method of the Chinese herbal medicine composition for preventing and treating the monozoic diseases of the fish.
The invention also aims to provide application of the Chinese herbal medicine composition for preventing and treating the monozoic diseases of the fish.
The purpose of the invention is realized by the following technical scheme:
a Chinese herbal medicine composition for preventing and treating monozoic diseases of fish is prepared from the following raw material medicines in percentage by weight: 5 to 10 percent of Polyporus umbellatus (Polyporus umbellatus), 25 to 40 percent of Notopterygium root (Notopterygium incsum), 10 to 20 percent of Lophatherum gracile (Lophatherum gracile), 15 to 25 percent of moutan bark (Paeonia suffruticosa) and 10 to 20 percent of Lithospermum erythrorhizon.
Preferably, the Chinese herbal medicine composition is prepared from the following raw material medicines in percentage by weight: 5-10% of polyporus umbellatus, 28-40% of notopterygium root, 17-20% of lophatherum gracile, 21-25% of moutan bark and 16-20% of lithospermum.
Preferably, the monozoiasis is neobrethreasis.
Preferably, the pathogen causing neobenidiasis is neobenidid meibomiae.
The preparation method of the Chinese herbal medicine composition for preventing and treating the monozoic diseases of the fish comprises the following operation steps:
(1) respectively drying the raw material medicines of the polyporus umbellatus, the notopterygium root, the lophatherum gracile, the moutan bark and the lithospermum erythrorhizon, crushing and sieving to obtain dry powder of each raw material medicine;
(2) mixing the dry powder of the raw material medicines in the step (1) according to the weight percentage to obtain the Chinese herbal medicine composition dry powder for preventing and treating the monozoic disease of the fish.
The drying in the step (1) is drying in an oven at 55-65 ℃;
the sieving mesh number in the step (1) is 200-300 meshes.
And (3) sealing the Chinese herbal medicine composition dry powder for preventing and treating the monozoite disease for the fish obtained in the step (2) by adopting a plastic bag, and storing the sealed dry powder in a dry place.
The preparation method of the Chinese herbal medicine composition for preventing and treating the monozoite for the fish can also comprise the following operation steps of:
(1) respectively drying the raw material medicines of the polyporus umbellatus, the notopterygium root, the lophatherum gracile, the moutan bark and the lithospermum erythrorhizon, crushing and sieving to obtain dry powder of each raw material medicine;
(2) mixing the dry powder of the raw material medicines in the step (1) according to the weight percentage, adding water for soaking, continuously decocting twice, filtering, and combining the filtrates to obtain a filtrate a;
(3) and (3) boiling the filtrate a obtained in the step (2), and concentrating to obtain the Chinese herbal medicine composition liquid preparation for preventing and treating the monozoic diseases of the fish.
The drying in the step (1) is drying in an oven at 55-65 ℃;
the screening mesh number in the step (1) is 40-60 meshes.
The volume of the added water in the step (2) is 15-30 times of the total weight of the mixed dry powder of the raw materials;
the soaking temperature in the step (2) is 60-80 ℃, and the time is 60-120 minutes;
the first decoction of the two times of decoction in the step (2) is carried out for 50-60 minutes by adopting slow fire, and the second decoction is carried out for 40-45 minutes by adopting slow fire;
the filtration in the step (2) is to filter by three layers of gauze and then filter by bolting silk.
The concentration of the Chinese herbal medicine composition liquid preparation for preventing and treating the monozoic disease for the fish in the step (3) is calculated according to 1 liter of Chinese herbal medicine composition liquid medicine for preventing and treating the monozoic disease for the fish, which is prepared from 1000g of raw material medicines.
The Chinese herbal medicine composition for preventing and treating the monozoic disease of the fish is applied to preparing feed additives or feeds for preventing and treating fish parasitic diseases.
The fish is preferably golden pompano or large yellow croaker.
The Chinese herbal medicine composition for preventing and treating the monozoite disease for the fish can play a role in preventing and treating the new neonystagmus disease no matter in a dry powder or liquid preparation. When in use, the Chinese herbal medicine composition dry powder or liquid preparation for preventing and treating the monozoite disease for the fish is added into the feed according to the mass percentage content of 0.5-6% to feed the fish, and the feeding amount of the feed is 2-5% of the weight of the fish.
The principle of the invention is as follows: in vitro experiments on each Chinese herbal medicine in the Chinese herbal medicine composition for killing the Meristotheca mume, the polyporus umbellatus, the notopterygium root, the lophatherum gracile, the moutan bark and the lithospermum have certain killing effect on the Meristotheca grandiflora. According to the literature report, the Chinese herbal medicine grifola is the dried sclerotium of the grifola of the family Polyporaceae, the main effective component of the grifola is grifola polysaccharide, and the grifola polysaccharide has the biological functions of resisting tumor and bacteria, enhancing the immunity of the organism, promoting urination and the like. Notopterygii rhizoma mainly contains volatile oil and coumarin compounds, and has antiinflammatory, analgesic, arrhythmia resisting, myocardial ischemia resisting, cerebral circulation promoting, thrombosis resisting, and antibacterial effects. The lophatherum gracile is the dried stem leaf of the herbaceous plant of the gramineae, the known chemical components of the lophatherum gracile mainly comprise flavonoids, and the modern pharmacology proves that the lophatherum gracile has the effects of relieving fever, promoting urination and resisting bacteria. Cortex moutan is derived from root bark of Paeonia suffruticosa Andr.f. of Ranunculaceae, and contains phenols and phenol glycosides, monoterpene and monoterpene glycosides, and triterpene, sterol and their glycosides, flavone, organic acid, coumarin, etc. as main chemical components, and has antiinflammatory, antibacterial, cardiovascular system regulating, central inhibitory, and immunity improving effects. The chemical components of lithospermum are mainly naphthoquinones, monoterpene phenol and benzoquinones, phenolic acid and salts thereof, alkaloids, aliphatic compounds and ester compounds, and the pharmacological activity of lithospermum mainly has the effects of bacteriostasis, inflammation resistance, virus resistance, liver protection, oxidation resistance, tumor resistance, immunoregulation and the like. The Chinese herbal medicines are combined together, so that the Chinese herbal medicine composition has the functions of preventing and treating diseases and regulating immunity. The proper proportion of the components of the various medicaments is determined by repeated tests of combinations with different proportions.
Compared with the prior art, the invention has the following advantages and effects:
(1) the Chinese herbal medicine composition for preventing and treating the monozoiasis of the fish has the effect of remarkably preventing and treating the neozoiasis of the fish, and is an ideal safe and green product for preventing and treating the monozoiasis of the fish, particularly the neozoiasis of the fish by oral administration; the composition enters the fish body in a feeding mode, has low toxicity to fish, is easy to degrade in the environment, has no residue, avoids the safety risk of aquatic product quality caused by chemical drug residue, ensures the water environment and the quality safety of aquatic products, and simultaneously reduces the consumption of manpower and material resources; is easy for industrialized production and popularization, and has wide application prospect in aquatic products.
(2) The raw material medicine of the invention has rich resources, simple and easy production method and low medicine cost, and is also suitable for self-made medicines prepared by self-prepared prescriptions.
Detailed Description
The present invention will be described in further detail with reference to examples, but the embodiments of the present invention are not limited thereto.
The host model fish used in the examples was golden pomfret (Trachinotus ovatus (Linnaeus)) and originated from the institute of aquatic products in south China sea. The parasite model was Neobedienia melleni (Neobedienia melleni), which originated from Hengxing fishery company, Zhanjiang province, Islands.
Example 1
(1) Drying the raw materials in an oven at 55 deg.C, pulverizing, and sieving with 200 mesh sieve to obtain dry powder of each raw material;
(2) uniformly mixing the dry powder of each raw material medicine obtained in the step (1) according to the following weight: 40g of polyporus umbellatus, 160g of notopterygium root, 85g of lophatherum gracile, 125g of moutan bark and 90g of lithospermum, thus obtaining the Chinese herbal medicine composition dry powder for preventing and treating the monozoic disease for the fish; the obtained Chinese herbal medicine composition dry powder for preventing and treating the monozoite for the fish is subpackaged and sealed by 0.5kg per bag by adopting a plastic bag and is stored in a dry place.
In this example, Polyporus is from Sichuan, Notopterygii rhizoma from Sichuan, Lophatherum gracile from Hunan, moutan cortex from Guangdong, and Arnebiae radix from Shandong.
Example 2
(1) Drying the raw materials in an oven at 60 ℃, crushing, and sieving with a 250-mesh sieve to obtain dry powder of each raw material; the sources of the raw materials of this example are the same as those of example 1;
(2) uniformly mixing the dry powder of each raw material medicine obtained in the step (1) according to the following weight: 25g of polyporus umbellatus, 200g of notopterygium root, 90g of lophatherum gracile, 105g of moutan bark and 80g of lithospermum erythrorhizon to obtain Chinese herbal medicine composition dry powder for preventing and treating monozoic diseases for fish; the obtained Chinese herbal medicine composition dry powder for preventing and treating the monozoite for the fish is subpackaged and sealed by 0.5kg per bag by adopting a plastic bag and is stored in a dry place.
Example 3
(1) Respectively drying the raw material medicines in an oven at 65 ℃, then crushing, and sieving with a 300-mesh sieve to obtain dry powder of each raw material medicine; the sources of the raw materials of this example are the same as those of example 1;
(2) uniformly mixing the dry powder of each raw material medicine obtained in the step (1) according to the following weight: 50g of polyporus umbellatus, 140g of notopterygium root, 100g of lophatherum gracile, 110g of moutan bark and 100g of lithospermum to obtain Chinese herbal medicine composition dry powder for preventing and treating monozoite diseases for fish; the obtained Chinese herbal medicine composition dry powder for preventing and treating the monozoite for the fish is subpackaged and sealed by 0.5kg per bag by adopting a plastic bag and is stored in a dry place.
Example 4
(1) Drying the raw materials in an oven at 55 deg.C, pulverizing, and sieving with 40 mesh sieve to obtain dry powder of each raw material; the sources of the raw materials of this example are the same as those of example 1;
(2) uniformly mixing the dry powder of each raw material medicine obtained in the step (1) according to the following weight: 80g of polyporus umbellatus, 320g of notopterygium root, 170g of lophatherum gracile, 250g of moutan bark and 180g of lithospermum, adding 15 liters of water, soaking at 60 ℃ for 120 minutes, decocting with slow fire for 60 minutes, adding 15 liters of water, decocting with slow fire for 45 minutes, filtering by using three layers of gauze, filtering by using bolting silk, and combining the filtrates to obtain a filtrate a;
(3) and boiling the filtrate a, and concentrating to obtain 1L of the Chinese herbal medicine composition liquid preparation for preventing and treating the monozoic diseases of the fish.
Example 5
(1) Drying the raw materials in an oven at 60 ℃, crushing, and sieving with a 50-mesh sieve to obtain dry powder of each raw material; the sources of the raw materials of this example are the same as those of example 1;
(2) uniformly mixing the dry powder of each raw material medicine obtained in the step (1) according to the following weight: 50g of polyporus umbellatus, 400g of notopterygium root, 180g of lophatherum gracile, 210g of moutan bark and 160g of lithospermum, 20 liters of water are added to soak for 100 minutes at 70 ℃, the mixture is decocted for 50 minutes by slow fire, 20 liters of water is added to be decocted for 40 minutes by slow fire, the mixture is filtered by three layers of gauze, then the filtration is carried out by using bolting silk, and the filtration is combined to obtain the filtration a;
(3) and boiling the filtrate a, and concentrating to obtain 1L of the Chinese herbal medicine composition liquid preparation for preventing and treating the monozoic diseases of the fish.
Example 6
(1) Respectively drying the raw material medicines in an oven at 65 ℃, then crushing, and sieving by a 60-mesh sieve to obtain dry powder of each raw material medicine; the sources of the raw materials of this example are the same as those of example 1;
(2) uniformly mixing the dry powder of each raw material medicine obtained in the step (1) according to the following weight: 100g of polyporus umbellatus, 280g of notopterygium root, 200g of lophatherum gracile, 220g of moutan bark and 200g of lithospermum, adding 30 liters of water, soaking at 80 ℃ for 60 minutes, decocting with slow fire for 50 minutes, adding 30 liters of water, decocting with slow fire for 40 minutes, filtering by using three layers of gauze, filtering by using bolting silk, and combining the filtrates to obtain a filtrate a;
(3) and boiling the filtrate a, and concentrating to obtain 1L of the Chinese herbal medicine composition liquid preparation for preventing and treating the monozoic diseases of the fish.
Example 7
(1) Preparing an ethanol extract of the Chinese herbal medicine composition: weighing 20g of the Chinese herbal medicine composition dry powder for preventing and treating the monozoic disease for the fish obtained in the embodiment 1, putting the dry powder into a Chinese herbal medicine extraction tank, adding 500mL of 95% ethanol, soaking and extracting for 24h at room temperature, and repeating for 3 times to obtain a total extracting solution; concentrating the total extractive solution under reduced pressure in a rotary evaporator, and drying the obtained concentrated solution in a 55 deg.C oven to constant weight.
(2) Preparing Chinese herbal medicine composition ethanol extract liquid medicine: weighing 2-5 mg of Chinese herbal medicine composition ethanol extract, placing the extract in a 2.0mL EP tube, dissolving the extract with filtered seawater (salinity of 30 per thousand) containing 0.1% dimethyl sulfoxide to prepare 2560mg/L of drug stock solution, and storing the drug stock solution in a refrigerator at 4 ℃ for later use. Adopts 2560mg/L of drug stock solution, and prepares drug solutions with different concentrations (1024, 512, 256, 128, 64, 32, 24, 16, 8 and 4mg/L) by using filtered seawater as working solution for standby.
(3) Detecting the in vitro activity of the imago of the newcastle disease: after adding 300. mu.L of seawater into each well of a 24-well plate, adult butterfish with neobenidiasis are carefully removed from the surface of the golden pomfret by forceps, 5 adults are placed into each well, 300. mu.L of liquid medicine with different concentrations are added respectively, so that the final concentration is 512, 256, 128, 64, 32, 16, 12, 8, 4, 2 and 0 (control, seawater group) mg/L, and 3 adults are placed in each concentration in parallel. The death time of the adults within 8h is counted under a 4-fold microscope.
The results show that: the ethanol crude extract of the Chinese herbal medicine composition has good killing activity on isolated Meretrix metschnikowii imagoes, and the minimum lethal concentration of the ethanol crude extract on the Meretrix metschnikowii imagoes within 8h is 128 mg/L.
Example 8
(1) Preparing a liquid preparation of the Chinese herbal medicine composition: 2.0mL of the liquid preparation of the Chinese herbal medicine composition for preventing and treating monozoiasis for fish obtained in example 4 was taken out and placed in an EP tube to serve as a drug stock solution having a concentration of 1000 g/L. Then preparing the raw liquid of the medicine into working liquids (200, 20, 2, 0.2, 0.02 and 0.002g/L) with different concentrations by using filtered seawater, and placing the working liquids in a refrigerator at 4 ℃ for later use.
(2) Detecting the in vitro activity of the imago of the newcastle disease: after adding 300. mu.L of seawater into each well of a 24-well plate, adult butterfish with neobenidiasis are carefully removed from the surface of the golden pomfret by forceps, 5 adults are placed into each well, 300. mu.L of working solution with different concentrations are added respectively, so that the final concentrations are 100, 10, 1, 0.1, 0.01, 0.001 and 0 (control, seawater group) g/L, and 3 adults are placed in parallel in each concentration. The death time of the adults within 8h is counted under a 4-fold microscope.
The results show that: the Chinese herbal medicine composition liquid preparation also has good killing activity on isolated Meretrix metschnikowii imagoes, and the minimum lethal concentration of the Chinese herbal medicine composition liquid preparation on the Meretrix metschnikowii imagoes within 8 hours is 1 g/L.
Example 9
(1) Preparing the Chinese herbal medicine composition feed: the Chinese herbal medicine composition dry powder for preventing and treating the monozoosis for the fish obtained in example 1 is added into a basic floating feed (purchased from Zhanjiang Henxing coral feeds Co., Ltd.) according to the proportion of 6% by mass, and is processed into floating granules, namely a Chinese herbal medicine composition feed, by a bait machine for later use. Wherein, the basic floatability feed is processed into floatability granular feed by a bait machine as the basic floatability granular feed.
(2) Experimental animals: healthy golden pomfret with the weight of 203.6 +/-18.1 g. Erecting 6 seawater net cages (4.0 × 4.0 × 4.5m) and dividing the seawater net cages into two groups, wherein one group is an experimental group, the other group is a control group, and each group is provided with 3 replicates; 800 golden pomfret fishes are put into each seawater net cage, and 50 marked diseased fishes infected with the new Benzenden meissner worm are put into the net cage for infection after 2-4 weeks of domestication. After 30 days, 200 golden pomfret branches are randomly selected, the body surface is detected, the average infection intensity of the new Benzenithan Meissner worm is determined to be 22.5 +/-3.2, and subsequent experiments are carried out.
(3) New treatment experiment of the neoniedriasis: the experimental group was fed with the herbal composition feed at a weight ratio of 2% of golden pompano weight twice a day at 9:00 and 16:00, and the control group was fed with the floating pellet feed (basic floating pellet feed) without the herbal composition. After the feed is continuously fed for 14 days, 40 golden pomfret fishes are respectively taken from each net cage, the number of new Meniere's disease insects on the body surface is counted after fresh water soaking, and the average infection intensity and the infection rate of the new Meniere's disease insects at each time point are calculated. Meanwhile, the death condition of the golden pomfret is counted during the experiment period, and the survival rate of the golden pomfret is calculated.
The average infection intensity is the total number of the Meyer new Benzender insects/the number of the fish infected with the Meyer new Benzender insects;
infection rate (%) × 100% (number of fish infected with newborns metschnikowii/total number of fish);
survival (%) × (number of live fish after experiment/total number of fish before experiment) × 100%;
the results show that: by feeding the Chinese herbal medicine composition feed, the effect of the golden pompano on resisting the Newcastle disease virus can be obviously improved, even the infection of the Newcastle disease virus can be completely eliminated, and meanwhile, the survival rate of the golden pompano is greatly improved.
From the average infection intensity and infection rate, the average infection intensity and infection rate of the control group of the new bentoniella mellifera at day 14 were 21.20 and 79.20%, respectively, while the average infection intensity and infection rate of the experimental group of the new bentoniella mellifera were 0. In addition, the survival rate of golden pomfret in the experimental group is up to 87.00 percent, which is obviously higher than that in the control group (49.20 percent).
Example 10
(1) Preparing the Chinese herbal medicine composition feed: the Chinese herbal medicine composition liquid preparation for preventing and treating the monozoic disease for the fish prepared in the example 4 is uniformly sprayed on the basic floating pellet feed by a spray can according to the proportion of 6% of the feed mass, and is dried in a drying oven at 55 ℃ to obtain the Chinese herbal medicine composition feed for standby.
(2) Experimental animals: the same as in example 9.
(3) New treatment experiment of the neoniedriasis: the experimental group was fed with the above Chinese herbal composition feed at a weight ratio of 2% of golden pompano weight twice a day at 9:00 and 16:00, and the control group was fed with a floating pellet feed (basic floating pellet feed) without Chinese herbal medicine added. After the feed is continuously fed for 14 days, 40 golden pomfret fishes are respectively taken from each net cage, the number of new Meniere's disease insects on the body surface is counted after fresh water soaking, and the average infection intensity and the infection rate of the new Meniere's disease insects at each time point are calculated. Meanwhile, the death condition of the golden pomfret is counted, and the survival rate of the golden pomfret is calculated. The average infection intensity, infection rate and survival rate of golden pompano of Newcastle disease were calculated in the same manner as in example 9.
The results show that: the feed of the Chinese herbal medicine composition can obviously improve the effect of the golden pompano on resisting the Newcastle disease, from the viewpoint of average infection intensity and infection rate, the average infection intensity and infection rate of the Newcastle disease at 14 days of a control group are respectively 19.5 and 86.2 percent, and the average infection intensity and infection rate of the Newcastle disease of an experimental group are both 0. In addition, the survival rate of golden pomfret in the experimental group is 82.97%, which is 42.80% higher than that in the control group (40.17%).
Example 11
(1) Preparing the Chinese herbal medicine composition feed: the Chinese herbal medicine composition liquid preparation for preventing and treating the monozoic disease for the fish prepared in the example 4 is uniformly sprayed on the basic floating pellet feed by a spray can according to the proportion of 20% of the feed mass, and is dried in a drying oven at 55 ℃ to obtain the Chinese herbal medicine composition feed for standby.
(2) Experimental animals: the total weight of healthy golden pomfret is 300, and the weight is 21.8 +/-3.3 g. Feeding the golden pomfret with basic floating pellet feed in a weight ratio of 2% of the golden pomfret weight twice at 9:00 and 16:00 respectively. And domesticating the artificial seawater (with salinity of 30 per mill) in the laboratory for 2-4 weeks.
(3) Toxicity test of the Chinese herbal medicine composition on golden pomfret: the experiment was divided into two groups, an experimental group and a control group, each group was provided with 3 replicates, each replicate of 50 tail pomfret. The experimental group was fed with the herbal composition feed at a weight ratio of 2% of golden pompano weight twice a day at 9:00 and 16:00, and the control group was fed with only the floating pellet feed (basic floating pellet feed) without the herbal composition. After continuously feeding the feed for 10 days, respectively taking 5 tails of golden pomfret, taking blood from tail vein, centrifuging at 3000r/min for 6min in a centrifuge, collecting serum, and determining activity of alanine Aminotransferase (ALT) and aspartate Aminotransferase (AST). Meanwhile, the death condition of the golden pomfret during the experiment is counted, and the survival rate of the golden pomfret is calculated.
Survival (%) × (number of live fish after experiment/total number of fish before experiment) × 100%;
the results show that: when the Chinese herbal medicine composition feed is fed to golden pomfret, the toxicity of the feed to golden pomfret is low, when the addition amount of a Chinese herbal medicine composition liquid preparation is 20%, the activities of glutamic-pyruvic transaminase and glutamic-oxaloacetic transaminase in the body of an experimental group at the 10 th day are 14.02U/L and 15.44U/L respectively, the activities of the glutamic-pyruvic transaminase and the glutamic-oxaloacetic transaminase in a control group are 13.83U/L and 16.14U/L respectively, and the two activities have no significant difference compared with each other. In addition, the death phenomenon of golden pomfret does not appear in the experimental process of 10 days in the experimental group, and the survival rate is the same as that of the control group and is 100 percent.
The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, substitutions, combinations, and simplifications are intended to be included in the scope of the present invention.
Claims (9)
1. A Chinese herbal medicine composition for preventing and treating monozoite diseases for fish is characterized in that: the Chinese herbal medicine composition is prepared from the following raw material medicines in percentage by weight: 5-10% of polyporus umbellatus, 25-40% of notopterygium root, 10-20% of lophatherum gracile, 15-25% of moutan bark and 10-20% of lithospermum;
the monozoiasis is new benidiasis.
2. The herbal composition for controlling monozoiasis in fish of claim 1, wherein: the Chinese herbal medicine composition is prepared from the following raw material medicines in percentage by weight: 5-10% of polyporus umbellatus, 28-40% of notopterygium root, 17-20% of lophatherum gracile, 21-25% of moutan bark and 16-20% of lithospermum.
3. A method of preparing a herbal composition for fish of any one of claims 1 to 2, comprising: the method comprises the following operation steps:
(1) respectively drying the raw material medicines of the polyporus umbellatus, the notopterygium root, the lophatherum gracile, the moutan bark and the lithospermum erythrorhizon, crushing and sieving to obtain dry powder of each raw material medicine;
(2) mixing the dry powder of the raw material medicines in the step (1) according to the weight percentage of any one of claims 1-2 to obtain the Chinese herbal medicine composition dry powder for preventing and treating the monozoic trematodes of the fish.
4. The method for preparing a herbal composition for controlling monozoiasis in fish according to claim 3, wherein the herbal composition comprises:
the drying in the step (1) is drying in an oven at 55-65 ℃;
the sieving mesh number in the step (1) is 200-300 meshes.
5. A method of preparing a herbal composition for fish of any one of claims 1 to 2, comprising: the method comprises the following operation steps:
(1) respectively drying the raw material medicines of the polyporus umbellatus, the notopterygium root, the lophatherum gracile, the moutan bark and the lithospermum erythrorhizon, crushing and sieving to obtain dry powder of each raw material medicine;
(2) mixing the dry powder of each raw material medicine in the step (1) according to the weight percentage of any one of claims 1-2, soaking in water, continuously decocting twice, filtering, and combining the filtrates to obtain a filtrate a;
(3) and (3) boiling the filtrate a obtained in the step (2), and concentrating to obtain the Chinese herbal medicine composition liquid preparation for preventing and treating the monozoic diseases of the fish.
6. The method for preparing a herbal composition for controlling monozoiasis in fish according to claim 5, wherein the herbal composition comprises:
the drying in the step (1) is drying at 55-65 ℃;
the screening mesh number in the step (1) is 40-60 meshes;
the volume of the added water in the step (2) is 15-30 times of the total weight of the mixed dry powder of the raw materials; wherein the volume is in liters and the weight is in kilograms;
the soaking temperature in the step (2) is 60-80 ℃, and the time is 60-120 minutes;
the first decoction of the two times of decoction in the step (2) is carried out for 50-60 minutes by adopting slow fire, and the second decoction is carried out for 40-45 minutes by adopting slow fire;
the concentration of the Chinese herbal medicine composition liquid preparation for preventing and treating the monozoic disease for the fish in the step (3) is calculated according to 1 liter of Chinese herbal medicine composition liquid medicine for preventing and treating the monozoic disease for the fish, which is prepared from 1000g of raw material medicines.
7. The use of a Chinese herbal composition for controlling monozoiasis in fish according to any of claims 1 to 2 for the preparation of a feed additive or feed for controlling parasitic diseases in fish, wherein:
the fish parasitic disease is neobenidiasis.
8. Use according to claim 7, characterized in that:
the fish is golden pomfret or large yellow croaker.
9. Use according to claim 7 or 8, characterized in that:
the Chinese herbal medicine composition for preventing and treating the monozoite disease of the fish is added into feed according to the mass percentage of 0.5-6%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911326795.4A CN110960635B (en) | 2019-12-20 | 2019-12-20 | Chinese herbal medicine composition for preventing and treating monozoite disease for fish and preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911326795.4A CN110960635B (en) | 2019-12-20 | 2019-12-20 | Chinese herbal medicine composition for preventing and treating monozoite disease for fish and preparation method and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110960635A CN110960635A (en) | 2020-04-07 |
| CN110960635B true CN110960635B (en) | 2021-07-27 |
Family
ID=70035518
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201911326795.4A Active CN110960635B (en) | 2019-12-20 | 2019-12-20 | Chinese herbal medicine composition for preventing and treating monozoite disease for fish and preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110960635B (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105250387A (en) * | 2015-11-04 | 2016-01-20 | 暨南大学 | Antibacterial Chinese herbal medicine compound for fishes as well as preparation method and application of antibacterial Chinese herbal medicine compound |
| CN106728444A (en) * | 2015-11-25 | 2017-05-31 | 天津市野龙港水产养殖科技发展有限公司 | A kind of Chinese medicine composition for preventing and treating fish fresh water ich |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106038682A (en) * | 2016-07-18 | 2016-10-26 | 暨南大学 | Chinese herbal medicine compound preparation for preventing and treating chthyophthirius multifilis for fish as well as preparation and application of Chinese herbal medicine compound preparation |
-
2019
- 2019-12-20 CN CN201911326795.4A patent/CN110960635B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105250387A (en) * | 2015-11-04 | 2016-01-20 | 暨南大学 | Antibacterial Chinese herbal medicine compound for fishes as well as preparation method and application of antibacterial Chinese herbal medicine compound |
| CN106728444A (en) * | 2015-11-25 | 2017-05-31 | 天津市野龙港水产养殖科技发展有限公司 | A kind of Chinese medicine composition for preventing and treating fish fresh water ich |
Non-Patent Citations (2)
| Title |
|---|
| 中草药对水产动物疾病防治的药效学研究综述;赵明军，等;《安徽农业科学》;20091231;第37卷(第36期);第18010-18015、18036页 * |
| 中西药合剂防治鲤单殖吸虫病的试验;毛世骏;《科学养鱼》;19981231(第4期);第23-24页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN110960635A (en) | 2020-04-07 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN100425258C (en) | Compound Chinese medicine composition and its prepn and use | |
| Fu et al. | Parasiticidal effects of Morus alba root bark extracts against Ichthyophthirius multifiliis infecting grass carp | |
| CN104887843B (en) | Traditional Chinese medicine composition for preventing and treating fish gill rot disease and preparation method thereof | |
| CN102987164B (en) | Megalobrama amblycephala compound Chinese medicinal herb anti-stress preparation, and preparation method and use method thereof | |
| CN105106495A (en) | Medicine for preventing and treating enteritis of fishes | |
| CN112674239A (en) | Chinese herbal medicine compound feed additive for fish and preparation method and application thereof | |
| CN106039138B (en) | Lactic acid bacteria traditional Chinese medicine fermentation antiviral preparation for aquaculture | |
| CN110960635B (en) | Chinese herbal medicine composition for preventing and treating monozoite disease for fish and preparation method and application thereof | |
| CN109316597B (en) | Traditional Chinese medicine preparation with antibacterial and liver-protecting functions for freshwater fish and preparation method thereof | |
| CN104543576A (en) | Compound plant-extract feed additive for preventing and treating piglet diarrhea, as well as preparation method and application thereof | |
| CN102028816B (en) | Chinese herbal medicinal compound preparation for treating hemorrhage disease of grass carp | |
| CN115212276B (en) | A topical Chinese herbal composition for preventing and treating ciliate disease of shrimp and crab | |
| CN103349758B (en) | The Chinese medicine preparation of a kind of aquatic animal hepatoprotective and preparation method | |
| JP4298043B2 (en) | Fish feed | |
| CN115624578A (en) | Traditional Chinese medicine composition for inhibiting aeromonas hydrophila and application thereof | |
| CN101755859A (en) | Traditional Chinese medicine composition for preventing parasite in water, preparation method and application thereof | |
| CN114681523A (en) | Chinese herbal medicine composition for effectively treating white spot disease of prawns | |
| CN107080770B (en) | Compound traditional Chinese medicine for preventing and treating fish dactylogyrus disease and preparation and use methods thereof | |
| CN107970303A (en) | A kind of herbal composite that can effectively treat the black and thin disease of turbot | |
| CN102640861A (en) | Fish feed containing traditional Chinese medicine component for preventing fish enteritis | |
| CN111265538A (en) | Application of periplocin in preparing medicine for killing fish ectoparasite | |
| Yuvaraj et al. | Immunomodulatory effects of seagrass Halophila ovalis polysaccharide mixed feed in adult black tiger shrimp Penaeus monodon and its protective efficacy against white spot syndrome virus infection | |
| CN102266438A (en) | Antiviral agent for freshwater fishes | |
| CN116173097B (en) | Traditional Chinese medicine composition for preventing and treating cryptocaryon irritans disease and application thereof | |
| CN106692483A (en) | Preparation capable of preventing and treating ascaris suum and preparation method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |