CN116173097B - Traditional Chinese medicine composition for preventing and treating cryptocaryon irritans disease and application thereof - Google Patents
Traditional Chinese medicine composition for preventing and treating cryptocaryon irritans disease and application thereof Download PDFInfo
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- CN116173097B CN116173097B CN202310143383.7A CN202310143383A CN116173097B CN 116173097 B CN116173097 B CN 116173097B CN 202310143383 A CN202310143383 A CN 202310143383A CN 116173097 B CN116173097 B CN 116173097B
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- A—HUMAN NECESSITIES
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- A23K—FODDER
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- A61K36/18—Magnoliophyta (angiosperms)
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- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/481—Astragalus (milkvetch)
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Abstract
The invention provides a traditional Chinese medicine composition for preventing and treating Cryptocaryon irritans, which comprises the following components: radix Sophorae Flavescentis, kochiae fructus, fructus Cnidii, radix astragali and Glycyrrhrizae radix. The Chinese medicinal composition has remarkable prevention and treatment effects on Cryptocaryon irritans, can improve the antioxidant capacity, immunity and intestinal digestive enzyme activity of fish bodies, is beneficial to enhancing the defensive capacity of organisms to external stimulus, and promotes the absorption and utilization of nutrient substances.
Description
Technical Field
The invention relates to a traditional Chinese medicine composition for preventing and treating cryptocaryon irritans and application thereof, and belongs to the technical field of cryptocaryon irritans prevention and treatment.
Background
The cryptocaryon irritans (Cryptocaryon irritans) are commonly called as 'sea water melon insects', belong to the cryptocaryophyllaceae (Family Cryptocaryonidae), and the cryptocaryon genus (Genus cryptocaryon) is a ciliated obligate parasite and mostly parasitize the gills, the fins, the body surfaces and other parts of tropical sea water fishes. After the fish body is infected with the cryptocaryon, the systemic distribution of the cryptocaryon is obvious, the white spot is commonly called as 'white spot disease', the disease has strong infectivity, wide transmissibility and higher infection rate and death rate, is a parasitic disease seriously endangering the mariculture fish, and causes huge economic loss for the raiser. At present, methods for controlling cryptocaryon irritans are roughly classified into physical methods and chemical methods. The method of the physical method mainly comprises the following steps: soaking in fresh water, heat treating, drying, converting culture, ultraviolet and ozone treating culture water, etc. However, these physical methods are complicated to operate and have unsatisfactory control effects. The chemical method mainly uses the compound to soak the diseased fish or the insect body to prevent and treat the Cryptocaryon irritans disease, and potassium permanganate, sulfanilamide thiazole, penicillin, copper sulfate, biological dye, formalin, acridine yellow, methylene blue, sodium hypochlorite, benzalkonium chloride and the like are all proved to be effective, can kill cysts, larvae and adults of the Cryptocaryon irritans, and has a certain effect on treating the disease. However, the long-term use of chemicals can reduce immunity and disease resistance of fish, and also can cause environmental pollution.
The traditional Chinese medicine composition is a natural medicine with the advantages of chemical structure diversity, rich biological activity and the like, has the characteristics of small toxic and side effect, safety, reliability, no pollution to the environment and the like, and has wide application prospect when being developed for preventing and treating Cryptocaryon irritans.
Disclosure of Invention
The invention provides a traditional Chinese medicine composition for preventing and treating Cryptocaryon irritans and application thereof, and the traditional Chinese medicine composition can effectively solve the problems.
The invention is realized in the following way:
a Chinese medicinal composition for preventing and treating Cryptocaryon irritans comprises the following components: radix Sophorae Flavescentis, kochiae fructus, fructus Cnidii, radix astragali and Glycyrrhrizae radix.
As a further improvement, the parts by weight of the kuh-seng, the fructus kochiae, the fructus cnidii, the radix astragali and the liquorice are respectively 20-40, 10-20, 20-40 and 5-15.
As a further improvement, the parts by weight of the kuh-seng, the belvedere fruit, the cnidium fruit, the astragalus root and the liquorice are respectively 30, 15, 30 and 10.
A feed comprises the above Chinese medicinal composition.
A medicament comprising the above-mentioned Chinese medicinal composition.
Application of the traditional Chinese medicine composition in preparing feed for preventing and treating Cryptocaryon irritans diseases.
Application of the traditional Chinese medicine composition in preparing medicines for preventing and treating cryptocaryon irritans diseases.
A method for preventing and treating Cryptocaryon irritans disease comprises applying the above Chinese medicinal composition.
A method for improving oxidation resistance of fish body comprises applying the above Chinese medicinal composition.
A method for improving immunity of fish body comprises applying the above Chinese medicinal composition.
A method for improving intestinal digestive enzyme activity of fish comprises applying the above Chinese medicinal composition.
The beneficial effects of the invention are as follows:
The traditional Chinese medicine composition for preventing and treating Cryptocaryon irritans has remarkable preventing and treating effects on Cryptocaryon irritans.
The traditional Chinese medicine composition for preventing and treating Cryptocaryon irritans can improve the oxidation resistance of fish bodies.
The traditional Chinese medicine composition for preventing and treating Cryptocaryon irritans can improve the immunity of fish bodies.
The traditional Chinese medicine composition for preventing and treating Cryptocaryon irritans can improve the intestinal digestive enzyme activity of fish.
The traditional Chinese medicine composition is a natural medicine and has the advantages of small toxic and side effects, safety, reliability and no pollution to the environment.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings that are needed in the embodiments will be briefly described below, it being understood that the following drawings only illustrate some examples of the present invention and therefore should not be considered as limiting the scope, and other related drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 shows the number of trophozoites on gills of each experimental group of white bars double saw fish according to the examples of the present invention.
FIG. 2 is the effect of the Chinese medicinal composition of example 6 of the present invention on the activity of LZM in serum of Lepidoptera albicans.
FIG. 3 shows the effect of the Chinese medicinal composition of example 6 on SOD activity of serum of Lepidoptera albi.
FIG. 4 shows the effect of the Chinese medicinal composition of example 6 on serum AKP activity of Bischonlein fish.
FIG. 5 is the effect of the Chinese medicinal composition of example 6 of the present invention on ACP activity in serum of Lepidoptera albicans.
FIG. 6 shows the effect of the Chinese medicinal composition of example 6 on liver SOD activity of Lepidoptera albi.
FIG. 7 is the effect of the Chinese medicinal composition of example 6 of the present invention on liver CAT activity of Lepidoptera albi.
FIG. 8 is the effect of the Chinese medicinal composition of example 6 of the present invention on liver T-AOC activity of Leptospermum grahami.
FIG. 9 is the effect of the Chinese medicinal composition of example 6 of the present invention on GSH-PX activity of liver of Lepidoptera.
FIG. 10 is the effect of the Chinese medicinal composition of example 6 of the present invention on intestinal trypsin activity of Bischawn fish.
FIG. 11 shows the effect of the Chinese medicinal composition of example 6 on the intestinal LPS activity of Lepidoptera albi.
FIG. 12 is the effect of the Chinese medicinal composition of example 6 of the present invention on the intestinal AMS activity of Lepidoptera albi.
Note that: in the above figures, the different lower case letters a\b\c\d represent significant differences between the test groups at the same time (p < 0.05).
Detailed Description
For the purpose of making the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is apparent that the described embodiments are some embodiments of the present invention, but not all embodiments. All other embodiments, based on the embodiments of the invention, which are apparent to those of ordinary skill in the art without inventive faculty, are intended to be within the scope of the invention. Thus, the following detailed description of the embodiments of the invention, as presented in the figures, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, based on the embodiments of the invention, which are apparent to those of ordinary skill in the art without inventive faculty, are intended to be within the scope of the invention.
The embodiment of the invention provides a traditional Chinese medicine composition for preventing and treating Cryptocaryon irritans, which comprises the following components: radix Sophorae Flavescentis, kochiae fructus, fructus Cnidii, radix astragali and Glycyrrhrizae radix.
The dried root of Sophora flavescens ait of Leguminosae is picked up in spring and autumn, the root head and small branch root are removed, and the product is cleaned, dried or sliced and dried while fresh. It is bitter and cold. Has effects of clearing heat, eliminating dampness, and killing parasites. Dried ripe fruit of Kochia scoparia (L.) kurz of Chenopodiaceae. Harvesting plants in autumn when the fruits are ripe, sun-drying, beating the fruits, and removing impurities. Has the main effects of clearing heat and promoting diuresis, dispelling wind and relieving itching and killing parasites. Fructus Cnidii, called wild carrot seed, is dry mature fruit of cnidium officinale Makino of Umbelliferae. Collected in summer and autumn when the fruit is ripe. Warm nature and bitter taste. Has the main effects of warming kidney, strengthening yang, eliminating dampness, dispelling wind and killing parasites. Astragalus root, radix astragali has the effects of invigorating qi and consolidating superficial resistance, and can improve the immunity of the body. Licorice detoxifies and harmonizes the drugs.
As a further improvement, the parts by weight of the kuh-seng, the fructus kochiae, the fructus cnidii, the radix astragali and the liquorice are respectively 20-40, 10-20, 20-40 and 5-15.
As a further improvement, the parts by weight of the kuh-seng, the belvedere fruit, the cnidium fruit, the astragalus root and the liquorice are respectively 30, 15, 30 and 10.
The embodiment of the invention provides a feed, which comprises the traditional Chinese medicine composition.
The embodiment of the invention provides a medicine, which comprises the traditional Chinese medicine composition.
The embodiment of the invention provides an application of the traditional Chinese medicine composition in preparing a feed for preventing and treating Cryptocaryon irritans.
The three medicines of kuh-seng, fructus kochiae and fructus cnidii have the effects of clearing heat, removing dampness, killing parasites, relieving itching and resisting inflammation. Astragalus root, radix astragali has the effects of invigorating qi and consolidating superficial resistance, and can improve the immunity of organisms. Licorice detoxifies and harmonizes the drugs. The invention contains various immunocompetence substances such as alkaloid, organic acid, polysaccharide and the like, and can improve phagocytic capacity of cells in an animal organism, promote organism metabolism and improve oxidation resistance so as to improve organism immunity. Therefore, the invention not only has the effects of preventing and treating the Cryptocaryon irritans, but also can improve the oxidation resistance, the immunity and the intestinal digestive enzyme activity of the fish body, is beneficial to enhancing the defending ability of the body against external stimulus and promotes the absorption and utilization of nutrient substances.
The embodiment of the invention provides an application of the traditional Chinese medicine composition in preparing a medicine for preventing and treating Cryptocaryon irritans.
The embodiment of the invention provides a method for improving the antioxidant capacity of fish bodies, and the traditional Chinese medicine composition is applied.
The embodiment of the invention provides a method for preventing and treating Cryptocaryon irritans disease, and the traditional Chinese medicine composition is applied.
The embodiment of the invention provides a method for improving immunity of fish bodies, which is applied to the traditional Chinese medicine composition.
The embodiment of the invention provides a method for improving the intestinal digestive enzyme activity of fish, which is applied to the traditional Chinese medicine composition. The Chinese medicinal composition can improve the activity of digestive enzymes in fish intestinal tracts, so that the digestion capacity of the fish intestinal tracts is improved, and the absorption of nutrient substances is promoted.
Example 1
Preparation of traditional Chinese medicine composition for preventing and treating cryptocaryon irritans
The formulation of the traditional Chinese medicine composition is shown in table1.
Table 1 Chinese medicinal composition formulation (g)
| Recipe number | Radix Sophorae Flavescentis | Kochia scoparia (L.) kudo | Fructus Cnidii | Radix astragali | Licorice root | Stemona root | Dried orange peel |
| 1 | 30 | 15 | 15 | 30 | 10 | 0 | 0 |
| 2 | 0 | 15 | 15 | 30 | 10 | 0 | 0 |
| 3 | 30 | 0 | 15 | 30 | 10 | 0 | 0 |
| 4 | 30 | 15 | 0 | 30 | 10 | 0 | 0 |
| 5 | 30 | 15 | 15 | 30 | 10 | 5 | 5 |
| 6 | 30 | 0 | 0 | 30 | 10 | 0 | 0 |
| 7 | 0 | 15 | 0 | 30 | 10 | 0 | 0 |
| 8 | 0 | 0 | 15 | 30 | 10 | 0 | 0 |
In addition, 2g of medicinal powder is weighed according to each formula, 400mL of seawater is added for decocting for 30min by slow fire, after cooling, sterilized seawater is used for quantitatively preparing 1g/L of mother liquor, and the mother liquor is placed at 4 ℃ for standby. 20g of each is soaked in 500ml of seawater for 30min and then decocted for 2h, and the gauze is used for filtering and concentrating the liquid medicine to 200ml, wherein the final concentration of the liquid medicine is 100mg/ml of the original medicine.
Example 2
Preparation of feed for preventing and treating cryptocaryon irritans disease
The Chinese medicinal composition prepared in example 1 was added to basic aquatic feeds (commercially available from Xiamen Huapu aquatic products development Co., ltd. (20210609.12)) in a ratio of 0g/kg (control), 5g/kg, 10g/kg, 20g/kg, 40g/kg, respectively, to prepare a slow-sinking pellet feed having a particle size of 1.5-2 mm.
Example 3
Experiment of killing Cryptocaryon irritans fish by oral administration of Chinese medicinal composition
The experimental fish is obtained from the aquatic institute of Xiamen Fujian province, and white double saw fish (Amphiprion frenatus) is selected, wherein the average body weight of the fish is (8.16+/-0.12) g, and the body length is (5.2+/-0.26) cm; the average body mass of the eye spot double saw fish (Amphiprion ocellaris) is (7.71+/-0.36) g, the body length is (5.3+/-0.19) cm, the average body mass of the blue fish (Epinephelus awoara) is (9.21+/-0.47) g, the body length is (8.63+/-0.31) cm, the eye spot double saw fish is temporarily cultured in a glass culture cylinder, feeds are respectively fed at 8:30 am and 2:30 pm every day, the test is started after the time lasts for 10 days, the water temperature in the culture cylinder is controlled at 26+/-1 ℃, the PH value is 7.5-8.1, the salinity is 30-32, the ammonia is 0.02mg/L, the nitrite is 0.01mg/L, and the dissolved oxygen is higher than 5.5mg/L. The seawater for cultivation is second-stage sand filtered seawater.
The Cryptocaryon irritans is a Cryptocaryon irritans ZP1 strain established in the laboratory, and the experimental strain passaging system is carried out by referring to methods such as learning (but learning, passage, preservation and prevention research of Cryptocaryon irritans (Cryptocaryon irritans) and Cryptocaryon disease, guangzhou: and Nansheng, 2006) by taking the garrupa spinosa (Epinephelus awoara) as an animal model so as to easily obtain the experimental Cryptocaryon irritans.
Collecting Cryptocaryon irritans cysts of which the body surfaces and gills fall off from the bottom of the culture tank, placing the Cryptocaryon irritans cysts into a seawater glass cup for hatching, and siphoning out hatched larvae for experiments.
Infection experiment: and (3) infecting white-striped double-saw fish, eye-spotted double-saw fish and green-stone-like fish with larvae newly hatched for 2 hours under dark conditions, wherein the infection concentration is 10000 per tail/L. When the mucus on the surface of the fish body and gill wires are observed under an optical microscope, the infected cryptocaryon irritans or the fish body can see the white spot are confirmed to be tested. The initial diseased fishes with small amount of white spots on the body surface and normal ingestion and swimming are randomly divided into 6 groups, wherein 5 groups are Chinese medicinal composition formula groups, 1 group is a control group, 3 parallel groups are arranged, and 30 fish are arranged in each parallel group.
The formula groups of the traditional Chinese medicine composition are respectively fed with feeds containing the traditional Chinese medicine compounds with 8 formulas of the example 1, and the concentration of the traditional Chinese medicine compounds is 20g/Kg. The control group was fed with feed without the addition of the Chinese medicinal composition. The feeding time is 8:30 am and 2:30 pm every day. The experimental container is a glass fiber reinforced plastic barrel with a conical bottom, 30% of water is changed every day after half an hour of bait feeding, and the immersed capsule and residual bait are discharged out of the barrel. The feeding and swimming states of the experimental fish are observed every day, the death quantity is recorded until all the control groups die, and the survival rate of the fish is calculated. The experimental results are shown in table 2.
TABLE 2 survival of experimental group fish (%)
| Experimental fish variety | White strip double saw fish | Eye-spot double saw fish | Blue-green groupers |
| Formulation 1 | 51.1 | 52.9 | 50.7 |
| Formulation 2 | 36.7 | 38.3 | 33.3 |
| Formulation 3 | 31.6 | 36.4 | 30.1 |
| Formulation 4 | 33.5 | 38.2 | 34.1 |
| Formulation 5 | 50.3 | 51.3 | 53.7 |
| Formulation 6 | 8.2 | 7.3 | 8.1 |
| Formulation 7 | 9.3 | 10.3 | 10.7 |
| Formulation 8 | 10.5 | 9.4 | 10.9 |
| Control group (Chinese medicinal composition) | 0 | 0 | 0 |
As can be seen from table 2, the survival rate of the fish of the formulations 1 and 5 is the highest, which means that the traditional Chinese medicine composition of the formulations 1 and 5 has the most remarkable killing effect on cryptocaryon irritans larva, and the effects of the formulations 1 and 5 are equivalent, which means that adding two medicines of radix stemonae and dried orange peel on the basis of the formulation 1 does not increase the efficacy, and the formulation 1 is the optimal formulation from the viewpoint of saving raw materials. In addition, the killing effect of the formulas 1 and 5 on the cryptocaryon irritans larva is obviously better than that of the formulas 2-4, and the sum of the effects of the formulas 6, 7 and 8 is lower than that of the formula 1, which shows that the three medicines of kuh-seng, fructus kochiae and fructus cnidii play a synergistic effect, and the killing effect on the cryptocaryon irritans larva is jointly played.
Example 4
Oral administration and medicated bath combined killing experiment of traditional Chinese medicine composition on Cryptocaryon irritans fish body infection
The culture of experimental fish and the establishment of cryptocaryon irritans were the same as in example 3.
Infected fish for experiment: white double sawas are infected with larvae newly hatched for 2 hours under dark conditions at an infection concentration of 10000 per tail/L. When the mucus on the surface of the fish body and gill wires are observed under an optical microscope, the infected cryptocaryon irritans or the fish body can see the white spot are confirmed to be tested. The initial diseased fish with small amount of white spots on the body surface and normal ingestion and swimming are experimental infected fish.
The experiment was divided into A, B, C, D groups of 3 parallels each, 30 fish per parallel group. Group A fed with feed containing 20g/Kg of the Chinese medicinal composition prepared in formula 1 of example 1, orally taken without medicated bath; group B was fed with a feed containing 20g/Kg of the Chinese medicinal composition prepared in formulation 1 of example 1, orally and simultaneously medicated bath (adding the Chinese medicinal composition to culture water) at 50mg/L of the Chinese medicinal composition; group C fed with feed containing 20g/Kg of the Chinese medicinal composition prepared in the formula 1 of example 1, and carrying out medicated bath orally and simultaneously according to 100mg/L of the Chinese medicinal composition; group D is control group, only basic feed is fed, and no Chinese medicinal composition is added. The medicated bath time is 19:00 a day to 7:00 a day the next morning; the feeding time is 8:30 am and 2:30 pm every day. The experimental container is a glass fiber reinforced plastic barrel with a conical bottom, 30% of water is changed every day after half an hour of feeding, and the immersed capsules and residual baits are discharged out of the barrel, so that the feeding management and the breeding environment during the experimental period are consistent with those during the temporary breeding period.
The feeding and swimming states of the experimental fish are observed every day, the death quantity is recorded until all the control groups die, and the survival rate of the fish is calculated. The experimental results are shown in table 3.
TABLE 3 Table 3
| Survival (%) | |
| Experiment A pharmaceutical group | 51.1 |
| Experiment B pharmaceutical group | 53.3 |
| Experiment C pharmaceutical group | 57.7 |
| Control group | 0 |
Experiment 0d, 2d, 4d and 6d, 3 fish were randomly picked up in each group, after anesthesia, the first gills on both sides were cut off to prepare water immersed pieces, and the number of trophozoites was counted under an optical microscope. The experimental results are shown in FIG. 1.
As shown in FIG. 1, the number of cryptocaryon irritans trophozoites on the first gill of fish was significantly reduced for A, B, C drug groups, with only 1-2 trophozoites per gill and no trophozoites observed on even some gills for each drug treatment group. The number of trophozoites on each gill of the control group is in a rapid rising trend, and the number of trophozoites is increased by more than 10 times. The experimental result shows that the traditional Chinese medicine composition flows into the whole body tissue and organs through blood absorption by oral administration, and has positive effects of preventing and treating insect pests. There was no significant difference in the number of cryptocaryon irritans trophozoites on gills between the oral group a and the oral + medicated bath B, C groups.
Example 5
The experimental fish were cultured as in example 3.
White double-sawed fish are randomly selected and put into test glass culture cylinders (L50 cm multiplied by W45cm multiplied by H40 cm) to carry out tests, 5 groups (0 g/kg (control), 5g/kg, 10g/kg, 20g/kg and 40 g/kg) are arranged in the tests, a traditional Chinese medicine composition formula 1 is used, 3 parallel samples of each group are used, 50 fish are fed continuously for 28 days in each cylinder, and the feeding management and culture environment during the tests are consistent with those during temporary culture.
During the experiment, 5 fish are randomly selected from each group of culture cylinders respectively at 7d, 14d, 21d and 28d, after 0.1mL/L eugenol is used for anaesthetizing the experimental fish, the tail is broken, blood is collected, the experimental fish is placed in a refrigerator at 4 ℃ for standing overnight after 1h, and centrifugation is carried out for 10min at 10000r/min, and the upper serum is taken; placing the fish on ice tray for dissection, and taking out liver and intestinal tissues. And (5) placing the serum and the tissue sample in a refrigerator at the temperature of-80 ℃ for standby. And detecting indexes such as serum, nonspecific immunity indexes in liver, intestinal digestive enzyme activity and the like. The experimental results are shown in FIGS. 2-12.
As shown in FIG. 2, the changes of Lysozyme (LZM) activity in serum samples of fish bodies were measured by feeding 0g/Kg, 5g/Kg, 10g/Kg, 20g/Kg, 40g/Kg of the Chinese medicinal composition. During the test, the LZM activity of the serum of the fish body can be increased and then decreased by feeding the Chinese medicinal composition groups with different doses. The activities of the 7d,5g/Kg and 40g/Kg drug groups are higher than that of the control group but the difference is not obvious (P is more than 0.05), and the activities of other drug groups are higher than that of the control group and the difference is obvious (P is less than 0.05). The LZM activity of each drug group from 14 to 28d is higher than that of the control group, and the difference is obvious. The LZM activity of the experimental group fed with 20g/Kg of drug added in 4 time sampling points is higher than that of the other drug groups. The LZM activity of 21d,20g/Kg drug group reaches the highest value. The LZM activity of 20g/Kg drug group was higher than that of the other drug groups at 4 time sampling points.
As shown in figure 3, the serum SOD activity of fish can be improved and reduced by feeding different doses of the Chinese medicinal composition. At 7d, the SOD activities of the 20g/Kg and 40g/Kg drug groups were significantly higher than those of the other drug groups and the control group, and the differences were significant (P < 0.05). At 14d, the SOD activity of the 20g/Kg drug group is significantly higher than that of other drug groups and control groups, and the difference is significant. The SOD activity of 21d,20g/Kg medicine group reaches the highest value and the difference between other medicine groups is obvious. 28d,20g/Kg of the drug group showed a decrease in SOD activity but still higher than that of the other drug groups, and the difference between the 20g/Kg of the drug group and the 5g/Kg of the drug group and the 10g/Kg of the drug group was not significant (P > 0.05), but the difference between the drug group and the 40g/Kg of the drug group was significant. The SOD activity of 20g/Kg of the medicine group is higher than that of other medicine groups in 4 time sampling points.
As shown in FIG. 4, the AKP activity in serum samples of fish bodies can be increased by feeding different doses of the Chinese medicinal composition, and the activity of each medicament group is obviously higher than that of a control group at different time points and the difference is obvious (P is less than 0.05). At 7d, the AKP activity was significantly higher in the 10g/Kg and 20g/Kg drug groups than in the other drug groups and the variability was significant. The AKP activity of the 14d,40g/Kg drug group is higher than that of other drug groups and the difference is obvious. The AKP activity of the 21d,20g/Kg drug group reached the highest value and was significantly different from the other 3 drug groups. 28d, the AKP activity of the other drug groups was decreased, and the 20g/Kg drug group was still higher than the other drug groups and the variability was significant, except for the 10g/Kg drug group.
As shown in FIG. 5, the ACP activity in serum samples of fish bodies can be increased by feeding different doses of the traditional Chinese medicine composition, and the activity of each medicine group is obviously higher than that of a control group at 4 time points and the difference is obvious (P is less than 0.05). During the test, the ACP activity of the serum of the fish body can be increased and then decreased in different doses of the traditional Chinese medicine composition groups. The three groups of drugs, except for the 5g/Kg drug group, showed significantly higher ACP activity than the control group and significant variability, but did not differ significantly between the three groups (P > 0.05). The 14d,40g/Kg drug group had ACP activity higher than the other drug groups and was significantly different. 21d,20g/Kg of the drug group showed the highest ACP activity and significant differences with other drug groups. The ACP activity of each drug group at 28d was decreased, and the ACP activities of the drug groups at 10g/Kg and 20g/Kg were higher than those of the other drug groups and were significantly different, but the difference between the two was not significant (P > 0.05).
As shown in FIG. 6, the SOD activity of the liver of the fish can be improved and reduced by feeding the Chinese medicinal composition at different doses, and the activity of each medicament group is obviously higher than that of a control group at 4 time points and the difference is obvious (P is less than 0.05). The SOD activities of the 7 th to 14d,20g/Kg and 40g/Kg drug groups are higher than those of other drug groups and have obvious differences, but the differences between the two drug groups are not obvious (P is more than 0.05). 21d,20g/Kg of the drug group reached the highest SOD activity and was significantly different from other drug groups. The SOD activity of each drug group at 28d is reduced. The SOD activity of 20g/Kg of the drug group is higher than that of other drug groups in 4 time sampling points.
As shown in FIG. 7, the CAT activity of the liver of the fish body can be improved and reduced by feeding the Chinese medicinal composition at different doses, and the activity of each medicament group is obviously higher than that of the control group at 4 time points and the difference is obvious (P is less than 0.05). The CAT activity of the 7 th to 14d,20g/Kg and 40g/Kg drug groups was significantly higher than that of the other drug groups and the differences were significant, but the differences were not significant (P > 0.05). 21d,20g/Kg drug group CAT activity reached the highest value and was significantly different from 5g/Kg and 10g/Kg drug group, but not from 40g/Kg drug group. The SOD activity of each drug group at 28d is reduced, but the CAT activity of the 20g/Kg drug group is still higher than that of other drug groups and the difference is obvious.
As shown in FIG. 8, the T-AOC activity of the liver of the fish can be improved and reduced by feeding the Chinese medicinal composition at different doses, and the activity of each medicament group is obviously higher than that of a control group at 4 time points and the difference is obvious (P is less than 0.05). The T-AOC activities of the 7 th to 14d,20g/Kg and 40g/Kg drug groups are higher than those of other drug groups and have obvious differences, but the differences between the two drug groups are not obvious (P is more than 0.05), and the T-AOC activities of the 20g/Kg drug groups reach the highest value and the differences between the other drug groups are obvious. Each drug group at 28d had reduced T-AOC activity, but the 20g/Kg drug group had still higher T-AOC activity than the other drug groups and was significantly different.
As shown in FIG. 9, the GSH-PX activity of the livers of the fishes can be improved and reduced by feeding the Chinese medicinal composition at different doses, and the GSH-PX activity of each medicinal group is obviously higher than that of the control group at 4 time points and the difference is obvious (P is less than 0.05). And the GSH-PX activity of each drug group is increased, and the GSH-PX activity of 20g/Kg drug group is higher than that of other drug groups and the difference is obvious in 7-14 d. 21d,20g/Kg of the drug group had GSH-PX activity up to the highest value and was significantly different from the other drug groups. The GSH-PX activity was decreased in each of the drug groups at 28 d. The T-AOC activity of the 20g/Kg drug group was higher than that of the other drug groups in the 4 time sampling points and the differences were significant.
As shown in FIG. 10, the intestinal trypsin activity of the fish can be increased by feeding different doses of the Chinese medicinal composition. The trypsin activity of each medicine group is not obvious from that of the control group (P is more than 0.05), the trypsin activity of each medicine group of 14-28d,0g/Kg, 5g/Kg and 10g/Kg is not obvious from that of the control group, the trypsin activity of each medicine group of 20g/Kg and 40g/Kg is obviously higher than that of the control group and the difference is obvious (P is less than 0.05), the intestinal trypsin activity of each medicine group of 21d,20g/Kg reaches the highest value, and the trypsin activity of each medicine group of 28d is reduced.
As shown in FIG. 11, the intestinal LPS activity of the fish can be increased by feeding different doses of the Chinese medicinal composition, and the activity of each medicament group is obviously higher than that of a control group at 4 time points and the difference is obvious (P is less than 0.05). The activity of each drug group was increased but not significantly (P > 0.05) at 7 d, and the difference in LPS activity between 3 drug groups of 21d,10g/Kg, 20g/Kg and 40g/Kg was significant but the difference in LPS activity between 3 drug groups was not significant, wherein the LPS activity of 20g/Kg drug group reached the highest value sampled at each time point. The 20g/Kg and 40g/Kg of the 28d drug group have significantly higher LPS activity than the other drug group and the control group, and the difference between the two groups is not significant.
As shown in FIG. 12, the intestinal AMS activity of fish can be increased by feeding different doses of the Chinese medicinal composition. The AMS activity was not significantly different between the drug and control groups at 7d (P > 0.05). AMS activity was elevated and differenced significantly from the control group (P < 0.05) for the 3 drug groups at 14, 21d,10g/Kg, 20g/Kg and 40g/Kg, but not between the 3 drug groups. AMS activity of 21d,20g/Kg drug group reached the highest value sampled at each time point. At 28d, the AMS activity of the other drug groups except for the 5g/Kg drug group started to decrease, and the AMS activity of each drug group was significantly higher than that of the control group and was significantly different, but the AMS activity difference between the drug groups was not significant.
In conclusion, the traditional Chinese medicine composition prepared in the embodiment 1 can improve the oxidation resistance of the fish body, the immunity of the fish body and the intestinal digestive enzyme activity, is beneficial to enhancing the defending ability of the body against external stimulus, promotes the absorption and the utilization of nutrient substances, and has positive prevention and treatment effects on the Cryptocaryon irritans. The best effect is achieved in the 20g/kg dose group with a feeding time of 14-21 d.
The above description is only of the preferred embodiments of the present invention and is not intended to limit the present invention, and various modifications and variations may be made to the present invention by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (3)
1. The traditional Chinese medicine composition for preventing and treating cryptocaryon irritans is characterized by comprising the following components: radix Sophorae Flavescentis, kochiae fructus, fructus Cnidii, radix astragali and Glycyrrhrizae radix; the weight portions of the kuh-seng, the belvedere fruit, the fructus cnidii, the astragalus and the liquorice are respectively 20-40, 10-20, 20-40 and 5-15.
2. The traditional Chinese medicine composition for preventing and treating cryptocaryon irritans according to claim 1, wherein the parts by weight of radix sophorae flavescentis, fructus kochiae, fructus cnidii, radix astragali and liquorice are 30, 15, 30 and 10 respectively.
3. Use of the traditional Chinese medicine composition according to claim 1 or 2 in the preparation of a medicament for preventing and treating cryptocaryon irritans.
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| 15 种中草药对刺激隐核虫(Cryptocaryon irritans)的杀灭效果及包囊破裂的条件;刘婷婷等;渔业科学进展;第36卷(第6期);113页 * |
| 八正散在皮肤病中的应用;王东庆;安徽中医临床杂志;-(03);211页左栏第1-2段 * |
| 王东庆.八正散在皮肤病中的应用.安徽中医临床杂志.2001,-(03),211页左栏第1-2段. * |
| 黄芪多糖和茯苓多糖对长江鲟幼鱼抗氧化指标、免疫指标和消化酶活性的影响;张建明等;黑龙江畜牧兽医(第2期);110页摘要 * |
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