CN1241608C - Antiviral, antibacterial and antiinflammatory herbal injection for treating livestock and fowl infectious disease - Google Patents
Antiviral, antibacterial and antiinflammatory herbal injection for treating livestock and fowl infectious disease Download PDFInfo
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Abstract
The present invention relates to an antiviral, antibacterial and anti-inflammatory herbal injection for treating infectious diseases of livestock and fowls. Honeysuckle, forsythia, coptis, phellodendron, scutellaria, gardenia, indigowood leaves and isatis roots are grouped, are decocted by water for extracting effective parts, and are refined for removing impurities such as tannin, protein, starch, resin, etc. The present invention has the advantages of no side effect and safe use. The cure rate of the injection to virulent infectious diseases and bacterial infectious diseases achieves more than 98%. Moreover, the infectious diseases are difficult to recur. Bacteria are difficult to generate medicine resistance performance. The present invention avoids the residue of veterinary medicine and social public hazard.
Description
Technical field:
The present invention relates to a kind of herbal medicine injection, particularly relate to a kind of antiviral anti-inflammation herbal medicine injection for the treatment of the poultry infectious disease.The field of traditional Chinese veterinary that belongs to treatment fowl bacterial and viral infectious.
Background technology:
Since nineteen nineties, China's livestock and poultry breeding industry is subjected to puzzlement external and traditional multiple infectious disease, especially poultry beasts viral infectious kind and quantity obviously increase, again not preferably medicine treat, cause bigger harm to aquaculture, traditional Chinese herbal medicine powder or decoction are difficult to obtain curative effect preferably, and the antibiotic Western medicine of some of prior art is only effective to bacterial infectious disease, and then effect is bad to virosis.And long-term abuse of antibiotics, sulfonamides and some chemical drugss make antibacterial produce behind the drug resistance that dosage can increase widely and curative effect descends to some extent.Not only increased feeding cost, also increased veterinary drug in of the harm of the intravital residual and toxic and side effects of poultry to the mankind.
Summary of the invention:
The object of the present invention is to provide a kind of antiviral anti-inflammation herbal medicine injection for the treatment of the poultry infectious disease, be used for the treatment of some fowl bacterial and viral infectious disease.Be a kind of pure injections of Chinese medicine, have no side effect, safe in utilization, improved the cure rate of poultry; Be difficult for recurrence with the back, and make antibacterial be difficult to produce drug resistance; Avoided the generation of residue of veterinary drug and social effects of pollution.
Technical scheme of the present invention is: the antiviral anti-inflammation herbal medicine injection of treatment poultry infectious disease, it is a kind of pure injections of Chinese medicine, by its prescription of monarch prescription be: Flos Lonicerae 15-60 gram, Fructus Forsythiae 10-60 gram, Rhizoma Coptidis 5-25 gram, Cortex Phellodendri 15-60 gram, Radix Scutellariae 15-60 gram, Fructus Gardeniae 15-60 gram, Folium Isatidis 10-60 gram, Radix Isatidis 10-60 gram;
Solubilizing agent is Tween 80 1-2%, and analgesic is a benzyl alcohol 0.5%.
The concrete process of preparing Chinese medicine of the present invention is extracted effective site for the grouping decocting, and concrete technology is as follows:
(1), add water after Rhizoma Coptidis powder is broken into coarse powder, add sulphuric acid, decoct and added acid continuously in 1-2 hour and decoct with water 3-5 time, extract sulphuric acid XIAONIE alkali salt, be concentrated into magma to pH value 1; Repeated hydrogenation calcium oxide liquid is transferred pH value to the 4 static supernatant of getting that stirs, and disposes with 95% ethanol liquid, and transferring concentration of alcohol is 60 degree, gets supernatant recovery ethanol, and taking liquid is standby;
(2), extract volatile oil and reclaim decocting liquid with Flos Lonicerae, Fructus Forsythiae decocting 3 times each 2 hours; Volatile oil need carry out being concentrated into after secondary distillation decocting liquid merges thick standby, transfers to 60 degree precipitations once with 95% ethanol earlier, and taking liquid is standby behind the recovery ethanol;
(3) surplus medicine was fried in shallow oil each 1-2 hour altogether, connected to fry in shallow oil 2-3 time, get be concentrated into after the decocting liquid merging thick.
It is made with extra care and goes the processing step of impurity such as tannin, albumen, starch, resin as follows:
(1) merge three kinds of magmas and add 95% ethanol and transfer to ethanol 60 degree, abundant stirring and evenly mixing, but precipitation get supernatant also filter pressing get filtrate recycling ethanol, but sediment reuse ethanol is disposed once; Reclaim behind the ethanol medicinal liquid and add 98% ethanol liquid again and stir evenly to the 70-80 degree, filtrate recycling ethanol and taking liquid are got in filter pressing;
(2) the dosing medicinal liquid adds injection water and volatile oil to 1: 1 ratio of crude drug weight and is every milliliter and contains crude drug 1 gram; Boil, cooling, cold preservation, quiescent setting is got and is cleaned precipitation, filters, cold and hot more in case of necessity disposal once can add active carbon and reduce phlegm and internal heat former and impurity when boiling, add fine straining behind the additives, the packing steam sterilization that flows promptly gets injection or oral liquid.
Above-mentioned medicament is injection and oral liquid.
Indication of the present invention comprises: infectious laryngotracheitis of chicken, infectious bronchitis, chronic respiratory disease, the multiple distemper sexually transmitted disease of fowl cholera and pig and dog.
Further specify curative effect of the present invention with regard to clinical effectiveness of the present invention below:
One, the external Mlc test of the present invention:
This test is test drug with the present invention, with pipemidic acid, trimethoprim, Western medicine such as pyrrole first mixture are contrast, each medicine all is diluted to 1: 5-1: 7 extension rates such as 320 grades are used, with ten kinds of antibacterials such as staphylococcus aureus is that strain carries out bacteriostatic test: the result shows that the present invention is to pasteurellosis bacillus, staphylococcus aureus, escherichia coli, bacillus enteritidis, shigella flexneri, the minimum inhibitory concentration of six kinds of antibacterials such as Bacillus proteus is doubly (6.3 μ l-3.1 μ l) best results of 160-320 dilution, minimum inhibitory concentration to bacillus typhi murium and Pullorum Disease bacillus is that doubly (50 μ l-25 μ l) effect is relatively poor in the 20-40 dilution, and the fungistatic effect of other three kinds of Western medicine contrasts all is starkly lower than this medicine, mostly is 5 dilutions doubly.
Two, paper disk method extracorporeal bacteria inhibitor test:
1, medicine: getting 0.5 milliliter of injection of the present invention, to add sterile saline to 10 milliliter standby, and it is standby to get the antibacterial scraps of paper of matched group penicillin and streptomycin and pipemidic acid, trimethoprim and pipemidic acid trimethoprim mixture; Pipemidic acid adds 10 milliliters of sterile salines with 32 milligrams, dissolves standby; Methoxy benzyl pyrimidine get 8 milligrams add water to 10 milliliters standby; Pyrrole first mixture is respectively got two medicines 32 and 8 milligrams and is added water to after 10 milliliters of dissolvings standby; The reuse diameter is 6 millimeters a test-paper, soaks completely above several drug solution respectively, dry for standby.
2, strain: nine kinds of antibacterials such as staphylococcus aureus, pasteurellosis bacillus, Bacillus proteus, bacillus typhi murium, bacillus enteritidis, escherichia coli, shigella flexneri, hog cholera bacillus, Pullorum Disease bacillus, above strain is provided by microorganism teaching and research room of veterinary university.
3, test method: antibacterial was cultivated 24 hours in ordinary broth and serum broth, getting this culture evenly coats on the plain agar culture medium, above-mentioned pastille test-paper is placed each plate culture medium centre respectively, each plate placed in the 37 degree constant incubators cultivated 24 hours, take out the inhibition zone diameter (mm) of each medicine of observed and recorded each bacterium.Its result such as following table:
Can know by last table result, fungistatic effect the best of the present invention, respectively contrast medicine apparently higher than other, differ highly significant (P<0.01) through comparing each medicine inhibition zone diameter, each medicine inhibition zone diameter puts in order and is the present invention (33.66mm)>streptomycin (22.66mm)>pipemidic acid (17.55mm), trimethoprim (17.77mm)>penicillin (15.55mm)>pyrrole first mixture (14.44mm).
Three, the present invention is to bacteriostatic test in the body of zoopery chicken Bacillus pasteurii disease:
1, with 80 of her sandgrouses of three monthly ages, be divided into four groups at random, 20 every group, prevention group promptly of the present invention, therapeutic test group, fowl cholera spirit treatment matched group, blank group.
2, strain is provided by Chinese veterinary drug feedstuff by the pasteurellosis bacillus freeze-drying lactobacillus that is provided, get strain one oese, place 20 milliliters of broth mediums to put in the 37 degree calorstats and cultivated 24 hours, calculating this bacterium liquid bacteria containing amount with a times times dilution method is every milliliter 32 * 10
8Individual antibacterial bottling sealing, refrigerator is preserved standby.
3, prevention group of the present invention: organize two ten chicken intramuscular injection with the dosage of 0.5ml to this by per kilogram of body weight earlier, every day secondary, logotype drug withdrawal after three days, give every subcutaneous injection bacterium liquid 0.5ml then, cause a disease for all the other animal subcutaneous injections to other the three groups dosage of all pressing 0.5ml bacterium liquid, connect behind the bacterium two hours, of the present invention group and chicken cholera spirit group are promptly treated, and dosage is tied medicine for the dosage of 10ml/kg body weight of the present invention and fowl cholera spirit 0.1 gram/kg body weight; Logotype three days, drug withdrawal was observed two to three days, and matched group is drug treatment not, observes four to five days, the young chicken that survives is slaughtered checked antibacterial in the body.Result such as following table:
The example number | Prevention group of the present invention | Treatment group of the present invention | Chicken cholera spirit treatment group | The blank group |
The inoculation number | 20 | 20 | 20 | 20 |
Cure number | 18 | 19 | 14 | 1 |
Death toll | 2 | 1 | 6 | 19 |
Survival rate | 90 | 95 | 70 | 5 |
Prevention group of the present invention as seen from the above table medication drug withdrawal in three days; see after the bacterial injection two young chicken morbidity death are arranged; survive 18; the prevention protective rate is 90%; and 19 of 20 young chicken deaths of blank group survive one, and survival rate is 5%; two groups relatively differ highly significant (P<0.01), show that the present invention has the good preventing effect to chicken cholera.Treatment group of the present invention is cured 19, and dead one, survival rate 95%, the chicken cholera spirit is cured 14, and dead six, survival rate relatively differs significantly (P<0.05) for 70%, two group, shows that treatment of the present invention is apparently higher than the chicken cholera spirit.
Four, the treatment of infectious bursal disease:
Get a group 22 ages in days that Shuangliao county power supply station raises, 450 of 1.5 jin, a traditional unit of weight of meat chickens of body weight are in heavenly died 22 with antibiotic therapy is invalid after the sudden onset, and complete group sickness rate 95% than 45 of severe disease examples, gently is 350.Be characterized as appetite and subtract greatly, fulminant diarrhoea occurs, be white in color and green loose stool; Be reluctant the walking of standing for sleeping inly, feather is fluffy, two wings are sagging, after death cut open inspection and see that lower limb, chest muscle have hemorrhage speckle, that hepatosplenomegaly, gallbladder elongate to become is big, glandular stomach is hemorrhage, fabricius bursa edema, volume increase 1-3 doubly, is full of brown cheese sample thing in the hemorrhage capsule of gauffer in the capsule, appearance is the Cera Flava color, according to epidemiology.Therapeutic Method: the sick chicken of serious symptom is used intramuscular injection of the present invention, and every each 1 milliliter, a twice-daily, logotype 5 days.The sick chicken of light disease is with the present invention's usefulness of drinking water, three days observed results of every 1.5 milliliters of successive administration such as following table:
Severe cases | Light disease case | Add up to | |
The treatment number | 45 | 350 | 395 |
Cure number | 45 | 350 | 395 |
Death toll | 0 | 0 | 0 |
Cure rate | 100 | 100 | 100 |
By in the table as can be known, the present invention treats 395 of infectious bursal diseases, all cures, do not see because of primary disease and secondary disease dead.
Five, the present invention is to the curative effect such as the following table of other infectious disease:
The sick kind | Infectious laryngotracheitis of chicken | Fowl cholera | Yellow scour of piglet | Baby swine paratyphoid | Canine distemper | Equine influenza |
The treatment number | 300 | 5400 | 110 | 11 | 35 | 33 |
Cure number | 295 | 5170 | 104 | 10 | 28 | 30 |
More do not count | 5 | 230 | 6 | 1 | 7 | 3 |
Cure rate % | 97.5 | 95.74 | 94.5 | 90.9 | 80 | 90.9 |
The present invention all has very good curative effect to treatment some infectious disease of pig such as chronic swine fever, pig lung plague, Schweineseuche, swine enzootic pneumonia, baby pig edema, Medulla sus domestica inflammation etc.
Good effect of the present invention is a kind of pure injections of Chinese medicine, is difficult for recurrence, and antibacterial is difficult to produce drug resistance; Have no side effect, safe in utilization, viral and cure rate bacterial infectious disease are reached more than 95%; Avoided the generation of residue of veterinary drug and social effects of pollution.
The specific embodiment:
The invention will be further described below in conjunction with specific embodiment:
Embodiment 1: prescription: Flos Lonicerae 15 grams, and Fructus Forsythiae 10 grams, Rhizoma Coptidis 5 grams, Cortex Phellodendri 15 grams, Radix Scutellariae 15 grams, Fructus Gardeniae 15 grams, Folium Isatidis 10 grams, Radix Isatidis 10 grams, solubilizing agent is a Tween 80 1%, analgesic is a benzyl alcohol 0.5%.
Embodiment 2: prescription: Flos Lonicerae 30 grams, and Fructus Forsythiae 20 grams, Rhizoma Coptidis 20 grams, Cortex Phellodendri 10 grams, Radix Scutellariae 30 grams, Fructus Gardeniae 30 grams, Folium Isatidis 20 grams, Radix Isatidis 20 grams, solubilizing agent is a Tween 80 1.5%, analgesic is a benzyl alcohol 0.5%.
Embodiment 3: prescription: Flos Lonicerae 45 grams, and Fructus Forsythiae 30 grams, Rhizoma Coptidis 15 grams, Cortex Phellodendri 45 grams, Radix Scutellariae 45 grams, Fructus Gardeniae 45 grams, Folium Isatidis 30 grams, Radix Isatidis 30 grams, solubilizing agent is a Tween 80 2%, analgesic is a benzyl alcohol 0.5%.
Above-mentioned three kinds of prescriptions decocting that divides into groups is extracted effective site, and concrete technology is as follows: (1), add water after Rhizoma Coptidis powder is broken into coarse powder, add sulphuric acid to pH value 1, decoct and added acid continuously in 1-2 hour and decoct with water 3-5 time, extraction sulphuric acid XIAONIE alkali salt is concentrated into magma; Repeated hydrogenation calcium oxide liquid is transferred pH value to the 4 static supernatant of getting that stirs, and disposes with 95% ethanol liquid, and transferring concentration of alcohol is 60 degree, gets supernatant recovery ethanol, and taking liquid is standby;
(2), extract volatile oil and reclaim decocting liquid with Flos Lonicerae, Fructus Forsythiae decocting 3 times each 2 hours; Volatile oil need carry out being concentrated into after secondary distillation decocting liquid merges thick standby, transfers to 60 degree precipitations once with 95% ethanol earlier, and taking liquid is standby behind the recovery ethanol;
(3) surplus medicine was fried in shallow oil each 1-2 hour altogether, connected to fry in shallow oil 2-3 time, get be concentrated into after the decocting liquid merging thick.
It is made with extra care and goes the processing step of impurity such as tannin, albumen, starch, resin as follows:
(1) merge three kinds of magmas and add 95% ethanol and transfer to ethanol 60 degree, abundant stirring and evenly mixing, but precipitation get supernatant also filter pressing get filtrate recycling ethanol, but sediment reuse ethanol is disposed once; Reclaim behind the ethanol medicinal liquid and add 98% ethanol liquid again and stir evenly to the 70-80 degree, filtrate recycling ethanol and taking liquid are got in filter pressing;
(2) the dosing medicinal liquid adds injection water and volatile oil to 1: 1 ratio of crude drug weight and is every milliliter and contains crude drug 1 gram; Boil, cooling, cold preservation, quiescent setting is got and is cleaned precipitation, filters, cold and hot more in case of necessity disposal once can add active carbon and reduce phlegm and internal heat former and impurity when boiling, add fine straining behind the additives, packing circulation steam sterilization promptly makes injection or oral liquid.
Claims (2)
1, the method for the herbal medicine of a kind of antiviral anti-inflammation for the treatment of the poultry infectious disease of preparation, make injection by following materials of weight proportions: Flos Lonicerae 15-60 gram, Fructus Forsythiae 10-60 gram, Rhizoma Coptidis 5-25 gram, Cortex Phellodendri 15-60 gram, Radix Scutellariae 15-60 gram, Fructus Gardeniae 15-60 gram, Folium Isatidis 10-60 gram, Radix Isatidis 10-60 gram
Its preparation method comprises the steps:
(1), add water after Rhizoma Coptidis powder is broken into coarse powder, adding sulphuric acid is 1 to pH value, decocts 1-2 hour, add acid continuously and decoct with water 3-5 time, extract berberine sulfate salt, be concentrated into magma, repeated hydrogenation calcium oxide liquid is transferred pH value to 4, stirring stirs evenly, and leaves standstill to get supernatant, and handles with 95% ethanol liquid, transferring concentration of alcohol is 60 degree, get supernatant, reclaim ethanol, it is standby to get medicinal liquid;
(2) with Flos Lonicerae, Fructus Forsythiae decocting 3 times, each 2 hours, extract volatile oil and reclaim decocting liquid, volatile oil carries out the secondary distillation, is concentrated into thick standbyly after decocting liquid merges, and transfers to 60 degree precipitations once with 95% ethanol, and taking liquid is standby behind the recovery ethanol;
(3) surplus medicine is fried in shallow oil altogether, each 1-2 hour, connect and fry in shallow oil 2-3 time, get be concentrated into after the decocting liquid merging thick standby;
(4) merge (1), (2), three kinds of standby medicine magmas of (3) step gained, add 95% ethanol and transfer to ethanol 60 degree, abundant stirring and evenly mixing, precipitation is got supernatant or filtrate is got in filter pressing, reclaims ethanol, and sediment reuse Ethanol Treatment is once; After reclaiming ethanol, medicinal liquid adds 98% ethanol liquid again and stirs evenly to the 70-80 degree, and filtrate is got in filter pressing, reclaims ethanol and taking liquid;
(5) dosing: medicinal liquid adds injection water and volatile oil and is equivalent to contain crude drug 1 gram to every milliliter, boil, cooling, cold preservation, staticly settle, go to clean precipitation, filter, cold and hot processing 1 like this or 2 times, adding active carbon when boiling reduces phlegm and internal heat former and impurity, fine straining behind the adding additives, packing, the circulation steam sterilization is promptly.
2, preparation method according to claim 1 is characterized in that described herbal medicine is made oral liquid.
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