CN106309761A - Pharmaceutical composition for treating avian infectious broncheitis and preparation method of microcapsules of pharmaceutical composition - Google Patents
Pharmaceutical composition for treating avian infectious broncheitis and preparation method of microcapsules of pharmaceutical composition Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/71—Ranunculaceae (Buttercup family), e.g. larkspur, hepatica, hydrastis, columbine or goldenseal
- A61K36/718—Coptis (goldthread)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/17—Gnetophyta, e.g. Ephedraceae (Mormon-tea family)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/19—Acanthaceae (Acanthus family)
- A61K36/195—Strobilanthes
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/31—Brassicaceae or Cruciferae (Mustard family), e.g. broccoli, cabbage or kohlrabi
- A61K36/315—Isatis, e.g. Dyer's woad
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/35—Caprifoliaceae (Honeysuckle family)
- A61K36/355—Lonicera (honeysuckle)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/70—Polygonaceae (Buckwheat family), e.g. spineflower or dock
- A61K36/704—Polygonum, e.g. knotweed
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/85—Verbenaceae (Verbena family)
- A61K36/855—Clerodendrum, e.g. glorybower
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/48—Preparations in capsules, e.g. of gelatin, of chocolate
- A61K9/50—Microcapsules having a gas, liquid or semi-solid filling; Solid microparticles or pellets surrounded by a distinct coating layer, e.g. coated microspheres, coated drug crystals
- A61K9/5005—Wall or coating material
- A61K9/5021—Organic macromolecular compounds
- A61K9/5036—Polysaccharides, e.g. gums, alginate; Cyclodextrin
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/70—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in livestock or poultry
Abstract
The invention discloses pharmaceutical composition for treating avian infectious broncheitis. The pharmaceutical composition is prepared from the following components in parts by weight: 0.1-1 part of ephedra extract, 0.1-1 part of indigowoad leaf extract, 0.1-2 parts of hypericum perforatum extract, 0.1-2 parts of honeysuckle flower extract and 0.1-2 parts of coptis extract. The invention also provides a preparation method of microcapsules of the pharmaceutical composition. The pharmaceutical composition has the beneficial effects as follows: the prepared traditional Chinese veterinary medicine microcapsules have quite good effect on treatment of the avian infectious broncheitis, a formula consists of natural products, and the traditional Chinese veterinary medicine microcapsules have effects of smell masking and sustained release and are not prone to producing residues, thereby having remarkable advantages. By means of in vitro tests and clinical pharmacodynamic tests, the clinical application effect of the traditional Chinese veterinary medicine composition and dosage form is determined, the application of more compound medicines is further popularized, a new approach is provided for development of high-quality products for corresponding enterprises, a new application field of the medicinal resources is expanded, a good basis is provided for research and development of new medicines for resisting livestock and poultry diseases and more obvious significance is realized.
Description
Technical field
The present invention relates to veterinary medicine technical field, be specifically related to a kind of medicine group for treating infectious bronchitis of chicken
Compound and the preparation method of microcapsule thereof.
Background technology
Herba Ephedrae is the medical herbs that " Chinese Pharmacopoeia " includes, and medicinal source is Ephedraceae plant plait Herba Ephedrae, epheday intermedia or Herba Equiseti Hiemalis fiber crops
Yellow dry herb stem.Tap the herb stem of green autumn, dry.It is warm in nature, acrid in the mouth, micro-hardship, has diaphoresis cold expelling, lung qi dispersing flat
Breathe heavily, effect of inducing diuresis to remove edema, anemofrigid cold, the diseases such as edema due to wind pathogen edema, bronchial asthma of breathing with cough uncomfortable in chest can be treated.Herba Hyperici perforati belongs to
In Guttiferae Hypericum one plant, for one of best-selling medical herbs in the whole world in recent years, recorded in American Pharmacopeia.I
State's traditional medicine regards Herba Hyperici perforati as clear vexatious photopic vision power, regulating menstruation network lively atmosphere blood, the good medicine of solution BAIDUXIAO inflammation.During modern times are a large amount of
Medicine pharmacological experiment proves that it can be with vasoconstrictive and then raising blood pressure, and function of gallbladder promoting relieving constipation, anti-inflammation etc. act on, and are used to treatment
Jaundice, urinary tract infection.Treat multiple disease, such as depression, tumor etc. in the world more.Herba Hyperici perforati has been incorporated into U.S.'s grass
Medicine pharmacopeia (AHP), very rare as medical herbs, it is often used as food additive, Herba Hyperici perforati extract is American market part
The occupation rate of volume improves year by year；In Germany, being used as antidepressant more than more than 100 years, Herba Hyperici perforati extractum preparation is
In Germany is anti-, the mild depression pharmaceutical market market share occupies nearly 50%.Its effective ingredient hypericin is sent out after deliberation
Be now the most active multifarious material in Herba Hyperici perforati extract, experimental results demonstrate, hypericin can treat depression,
Suppression tumor, anti-DNA and RNA virus.Folium Isatidis has antibacterial action that virus etc. is also had inhibitory action.Folium Isatidis also can be right
The effect of anti-escherichia coli endotoxin.Polygonaceae and Cruciferae Folium Isatidis decoct have slight antiinflammatory action to animal, reduce capillary
Vascular permeability, increases leukocytic phagocytosis, and polygonum tinctorium ait. leaf decoct can suppress isolated rabbit intestinal, and excited isolated guinea-pig uterus, to family
Rabbit also has refrigeration function.Indirubin has antitumaous effect.Effect of Rhizoma Coptidis, heat clearing and damp drying, eliminating fire and detoxication.For damp and hot feeling of fullness, vomit
Telling, dysentery, jaundice, unconsciousness due to high fever, hyperactivity of heart-fire, dysphoria and insomnia, heat in blood tells nosebleed, conjunctival congestion acid regurgitation, toothache, quenches one's thirst, carbuncle furuncle；
External treatment eczema, eczema, auditory meatus is suppurated.It is burning hot that Rhizoma Coptidis (processed with wine) is apt to the clear part of the body cavity above the diaphragm housing the heart and lungs.For conjunctival congestion, aphtha.Prepared RHIZOMA COPTIDIS with rhizoma zingiberis recens juice clearing stomach stomach function regulating preventing or arresting vomiting.
Microcapsule refers to a kind of miniature vessel with polymeric shell wall or packing material, has the micro-of semi permeability or sealing
Small particles, the most wrapped material is referred to as core, and the material of parcel core is referred to as wall material.The research of microcapsule technology starts from 20
The 30's of century, United States Atlantic Coast fishery the method preparing cod-liver oil microcapsule proposed.The U.S. in 1954
The Green of NCR company uses complex coacervation successfully to prepare the gelatin-microcapsule of oil-containing, and is used for preparing carbonless copying
Paper.The state of material, quality, volume and performance can be changed owing to having, protect sensitive composition, enhanced stability, control core
Material discharges, and reduces or covers disagreeable taste, reduces volatility, the isolation function such as component, microcapsule technology at food, medicine, spin
Knit, the aspect such as coating, agricultural, cosmetics industry is widely used.Along with the development of microcapsule technology, microcapsule technology has
Many new application, such as phase-change microcapsule, lasting microcapsule, self-repairing microcapsule, slow-release microcapsule, capsule of nano etc.,
Significantly expand the range of application of microcapsule technology.
Summary of the invention
The purpose of the present invention is aiming at above-mentioned defect of the prior art, it is provided that one is used for treating avian infectious
The pharmaceutical composition of tracheitis and the preparation method of microcapsule thereof.
To achieve these goals, the technical scheme that the present invention provides is: one is used for treating infectious bronchitis of chicken
Pharmaceutical composition, be to be prepared by following components in parts by weight: Herba Ephedrae extract 0.1-1 part, Folium Isatidis carry
Take thing 0.1-1 part, Herba Hyperici perforati extract 0.1-2 part, Flos Lonicerae extract 0.1-2 part, Rhizoma Coptidis extract 0.1-2 part.
Further, above-mentioned a kind of pharmaceutical composition for treating infectious bronchitis of chicken, is by according to weight
The following components of part meter prepares: Herba Ephedrae extract 0.5 part, Folium Isatidis extract 0.1 part, Herba Hyperici perforati extract 1
Part, Flos Lonicerae extract 2 parts, Rhizoma Coptidis extract 0.1 part.
Further, above-mentioned a kind of pharmaceutical composition for treating infectious bronchitis of chicken, it is characterised in that institute
The dosage form stating pharmaceutical composition is microcapsule and any one dosage form conventional.
Second object of the present invention there is provided above-mentioned a kind of drug regimen for treating infectious bronchitis of chicken
The preparation method of thing microcapsule, weighs each component as core according to weight, adds a small amount of anhydrous alcohol solution, progressively
It is added in the mixture of the beta-schardinger dextrin-as wall material and modified starch, after colloid mill grinds, at 4 DEG C, balances 12 h, reclaim
Ethanol, i.e. obtains the pharmaceutical composition microcapsule for treating infectious bronchitis of chicken after filtration drying.
Further, above-mentioned preparation method, the core content in described microcapsule is 10-in percentage by weight
60%；The grinding rotating speed of described colloid mill is 1200r/min, and grinding temperature is 20 DEG C-60 DEG C, and colloid mill milling time is 10-
60min；The mass ratio of described beta-schardinger dextrin-and modified starch is (1-6): (1-6).
Further, above-mentioned preparation method, the core content in described microcapsule is in percentage by weight
30%；The grinding temperature of described colloid mill is 30 DEG C, and colloid mill milling time is 30min；Described beta-schardinger dextrin-and modified starch
Mass ratio is 4:3.
At embodiments of the invention test portion, pharmaceutical composition is prepared as injection, it is true that medicine of the present invention
Dosage form is not limited to herbal medicine microcapsule, it is possible to use conventional adjuvant to be made into the dosage form such as tablet, powder.Different dosage form medicine
The therapeutic effect of thing is close.
The invention have the benefit that the present invention be by Herba Ephedrae extract, Folium Isatidis extract, Herba Hyperici perforati extract,
Flos Lonicerae extract, Rhizoma Coptidis extract carry out prescription and prepare herbal medicine microcapsule, are used for treating infectious bronchitis of chicken, have
Extraordinary effect, prescription is made up of natural product, and herbal medicine microcapsule has taste masking, slow releasing function, and is difficult to residual occur, tool
Have a clear superiority in, by vitro tests and the clinical test of pesticide effectiveness, determine that the Chinese veterinary medicinal composition of the present invention and the clinic of dosage form are answered
By effect, for the more polypharmaceutic application of further genralrlization, the product for corresponding enterprise development high-quality provides new way, expands
The frontier of drug resource application, researches and develops anti-poultry disease new drug and provides good basis, have obvious meaning.
Detailed description of the invention
Embodiment 1:
Formulation content and source used in the present invention are as shown in table 1.
Table 1
A kind of preparation method for treating infectious bronchitis of chicken herbal medicine microcapsule:
Chinese veterinary drug preparation complicated component, and most composition is unclear, mechanism of action is indefinite, owing to poultry are administered custom and use
Conventional formulation technique, causes formulation aesthetics, thick, big, black, and onset is slow, and difficult quality control etc., this not only makes livestock breeding industry
The consciousness resisting herbal medicine strengthens, and also directly results in herbal medicine competitiveness disappearance in the world, and herbal medicine is cured the disease multiplex compound recipe,
Modern pharmacology research proves, it has Multiple components and multiple target spot, feature, uses high new science pharmaceutical technology, in research
Veterinary drug criterion composition and the mechanism of action are also produced by International Drug standards, on the basis of respecting herbal medicine medication theory,
Agent is carried out for the purpose of improving Chinese veterinary drug preparation clinical efficacy, reducing toxic and side effects, expansion poultry route of administration and medication poultry
Type reform and preparation research, be to strengthen herbal medicine international competitiveness, it is achieved the key of Chinese veterinary drug preparation modernization, Chinese veterinary drug preparation
Research be domestic many scholars still in the problem tried to explore, how to solve effect and the analysis of herbal medicine compound recipe Multiple components,
Set up the research method optimizing prescription.It is connected theoretical for herbal medicine mutually with modern times composition of prescription, is need from now on to face great
Research topic, the dynamic change of herbal medicine effective ingredient, folk prescription, compound recipe process in vivo, herbal medicine compound recipe can be directly affected and join
The mechanism of action of 5.Summing up a kind of can integrating with and the theory of not disalignment veterinary pharmaceutical true qualities with international animal medicine, this is
The bottleneck problem that can Chinese veterinarian's pharmacy go to the world, modern herbal medicine research field is achieved with the one-tenth much attracted people's attention
Really, the main new concept that this field scholar praises highly has molecule herbal medicine, the molecule pharmacognosy, herbal medicine chemical group etc..Permitted
Many pharmacy new and high technology is applied in Chinese veterinary drug preparation preparation process, improves Chinese veterinary drug preparation quality and biological utilisation
Degree.
At present, most herbal medicine effective ingredient all also exist the problem such as poor stability, heavy bitter taste, and micro encapsulation can be very
Protect well pigment from condition oxidations such as photo-thermal, decompose and taste masking, thus improve its stability and fowl oral application.This patent
Use polishing to prepare the preparation method of compound traditional Chinese veterinary medicine microcapsule, and optimize its process conditions, extensive for compound traditional Chinese veterinary medicine
Application provides a kind of novel formulation.
1 materials and methods:
1.1 materials and reagent:
Reagent: the reagent such as industrial alcohol (95%), modified starch, beta-schardinger dextrin-are domestic analytical pure.
1.2 instrument and equipments:
JM-LB type auspicious vast rustless steel vertical hygiene-type colloid mill: auspicious vast (Shanghai) stainless steel equipment company limited；
ABBDHG-9240A type electric vacunm drying case: above Nereid's grand testing equipment company limited；
JA2003 electronic balance: above current chart level instruments and meters company limited；
DK-98-1 type electric hot water bath: Tianjin Stettlen Instrument Ltd.；
UV-2450 type ultraviolet-visible spectrophotometer: Japan's Shimadzu.
1.3 methods:
1.3.1 the prescription of Chinese medicine extract:
Herba Ephedrae extract 1kg, Folium Isatidis extract 1kg, Herba Hyperici perforati extract 1kg, Flos Lonicerae extract 1kg, Rhizoma Coptidis is extracted
Thing 1kg, by above extract in 10L stainless steel cask, stirs 30min by stirring rod, after uniformly, standby.
1.3.2 the preparation of compound traditional Chinese veterinary medicine microcapsule:
Microcapsule is prepared with colloid milling.Weigh a certain amount of compound traditional Chinese veterinary medicine with a small amount of anhydrous alcohol solution, be progressively added to one
Determine concentration 20%(W/W) and the beta-schardinger dextrin-of ratio and modified starch in, after colloid mill work certain time, balance at 4 DEG C
12 h, reclaim ethanol, after filtration, the most i.e. obtain compound traditional Chinese veterinary medicine microcapsule.Then by after the pigmentolysis of surface of microcapsule
Filter constant volume, measure absorbance and calculate embedding rate, weigh micro encapsulation degree.
1.3.3 compound traditional Chinese veterinary medicine micro encapsulation single factor experiment:
1.3.3.1 the mass ratio of core and the wall material impact on compound traditional Chinese veterinary medicine microcapsule:
The content (W/W) of compound traditional Chinese veterinary medicine core is respectively 10%, 20%, 30%, 40%, 50%, 60%.Wall material beta-schardinger dextrin-and degeneration
The mass ratio of starch is 43, and under the conditions of 30 DEG C, colloid mill works 30 min, recycling design, dried microcapsule, measures bag
Bury rate.
1.3.3.2 the grinding temperature impact on compound traditional Chinese veterinary medicine microcapsule stability:
Colloid mill operating temperature is: 20 DEG C, 30 DEG C, 40 DEG C, 50 DEG C, 60 DEG C, and core is 3:7 with the mass ratio of wall material, and β-ring is stuck with paste
Essence and the mass ratio of modified starch are to grind 30 min under conditions of 4:3, prepare microcapsule, measure embedding rate.
1.3.3.3 the colloid mill working time impact on compound traditional Chinese veterinary medicine stability:
The colloid mill working time is: 10 min, 20 min, 30 min, 40min, 50 min, 60 min, the matter of core and wall material
Amount is 4:3 than the mass ratio for 3:7, beta-schardinger dextrin-and modified starch, in temperature is: prepare microcapsule under conditions of 30 DEG C, surveys
Determine embedding rate.
1.3.3.4 the mass ratio of beta-schardinger dextrin-and the deformation starch impact on herbal mixture stability:
The mass ratio of beta-schardinger dextrin-and modified starch is respectively (1:6), (2:5), (3:4), (4:3), (5:2), (6:1), core
It is 4:3 with the mass ratio of wall material, under the conditions of 30 DEG C, grinds 30 min, prepare microcapsule, measure embedding rate.
1.3.4 compound traditional Chinese veterinary medicine microcapsule microcapsule orthogonal test:
The mass ratio 3 of colloid mill operating temperature, time, modified starch and beta-schardinger dextrin-is chosen on the basis of single factor experiment
Main influence factor, with embedding rate as measurement index, uses Orthogonal Experiment and Design, respectively maize yellow-powder pigment is carried out micro-glue
Capsule is tested.Compound traditional Chinese veterinary medicine microcapsule microcapsule orthogonal test factor level is as shown in table 2.
Table 2
The calculating of the most micro-microcapsule embedded rate of glue compound traditional Chinese veterinary medicine:
The absorbance of compound traditional Chinese veterinary medicine microcapsule and the proportional relation of herbal medicine concentration.Therefore this test is first using absorbance as weighing apparatus
The index of amount compound traditional Chinese veterinary medicine content, calculates the embedding rate of micro encapsulation herbal mixture by absorbance.
Compound traditional Chinese veterinary medicine embedding rate=(total amount of the concentration of 1-microcapsule compound traditional Chinese veterinary medicine/microcapsule pigment) × 100%
(1).
2 results and analysis:
2.1 compound traditional Chinese veterinary medicine microcapsule single factor experiment result and analyses:
2.1.1 the proportioning of core and the wall material impact on micro encapsulation:
It is above what colloid mill rotating speed completed under 1200r/min, as shown in Table 3, along with being gradually increased of core content, compound recipe
The embedding rate of herbal medicine microcapsule is gradually lowered, and it is the biggest that this is possibly due to wall material ratio, and the wall forming microcapsule is the thickest, capsule
The fastness of wall is increased by, and embedding rate just increases.But when pigment content is too low, it is likely to be due to wall material amount excessive, causes micro-
Capsule wall is blocked up, and maize yellow-powder pigment embedding amount is very few, and is not suitable in practice production, when core content 30%, and embedding
Rate is more than 80%, so according to the color valency of product after micro encapsulation and dissolubility, selecting core content is 30% as optimal
Consumption.The impact of core wall comparison compound traditional Chinese veterinary medicine micro encapsulation embedding rate is as shown in table 3.
Table 3
2.1.2 the grinding temperature impact on micro encapsulation:
As seen from the results in Table 4, when grinding temperature is 20 DEG C～60 DEG C, the embedding rate of micro encapsulation raises with temperature and increases.
This is because when grinding temperature raises, wall material film forming speed can be made to accelerate, improve embedding effect.But when temperature continues to raise
Time, cause the film property of wall material to reduce, microencapsulated oil powder reduces, impact embedding effect.But when temperature is too high, due to color
Element loss embedding rate reduces, the therefore microcapsule best results when temperature is 30 DEG C.Grinding temperature is to compound traditional Chinese veterinary medicine microcapsule
The impact changing embedding rate is as shown in table 4.
Table 4
2.1.3 the milling time impact on micro encapsulation:
From table 5, along with the prolongation of milling time, embedding rate improves, but when 30 more than min, but occurs under embedding rate
The situation of fall.This is because prolongation over time, it is the most that Traditional Chinese veterinary medicine extract molecule enters wall material intramolecular, when in wall material and
After Traditional Chinese veterinary medicine extract fully combines, increasing milling time pigment can slightly lose, and embedding rate is by a declining curve.Therefore,
Suitable milling time is 30 min.Milling time is as shown in table 5 on the impact of compound traditional Chinese veterinary medicine micro encapsulation embedding rate.
Table 5
2.1.4 beta-schardinger dextrin-and the impact on micro encapsulation of the modified starch mass ratio:
As shown in Table 6, the difference of wall material ratio produces impact to microcapsule embedded rate, and this is possibly due to modified starch and has breast
Change and embedding effect, and cyclodextrin is used as microcapsule wall material and has synergism, promote the formation of microcapsule, and and core combination
Ratio is stronger.Result shows, when modified starch is 43 with cyclodextrin mass ratio, microcapsule effectiveness comparison is good.Beta-schardinger dextrin-and change
The mass ratio of property starch is as shown in table 6 on the impact of compound traditional Chinese veterinary medicine micro encapsulation.
Table 6
2.2 micro encapsulation compound traditional Chinese veterinary medicine orthogonal experiments and analyses:
Choose 3 main influence factors of mass ratio of grinding temperature, time, modified starch and beta-schardinger dextrin-, with embedding rate be
Measurement index carries out Orthogonal Experiment and Design, determines the optimum condition of compound traditional Chinese veterinary medicine micro encapsulation.Different microcapsule experimental conditions
The embedding rate of lower gained the results are shown in Table 7.
From table 7 result of the test it can be seen that range analysis is A ＞ B ＞ C, the optimum condition of compound traditional Chinese veterinary medicine micro encapsulation
Being 30 min for A3B2C1, i.e. milling time, grinding temperature is 40 DEG C, and the mass ratio of modified starch and beta-schardinger dextrin-is 34
Time, the embedding rate of compound traditional Chinese veterinary medicine is 90.5% to the maximum.
Table 7
3 conclusions:
This research uses polishing to inquire into the micro-encapsulation technology of compound traditional Chinese veterinary medicine, draws compound traditional Chinese veterinary medicine micro encapsulation
Process conditions be:
Use polishing that the micro-encapsulation technology of compound traditional Chinese veterinary medicine is inquired into,
Core content 10-60%(W/W), optium concentration is 30%.
Grinding temperature is when 20 DEG C-60 DEG C, and optimum temperature is 30 DEG C.
The colloid mill working time is: 10-60 min, Best Times 30 min.
The mass ratio of beta-schardinger dextrin-and modified starch is respectively as follows: 1-6:1-6, and optimum quality ratio is 43.
The embedding rate of compound traditional Chinese veterinary medicine is 84.5% to the maximum.
As can be seen here, the compound traditional Chinese veterinary medicine after micro encapsulation, in wall material modified starch and the embedding effect of beta-schardinger dextrin-
Under, compound traditional Chinese veterinary medicine is fully embedded, and its stability can be greatly improved, and this research is the actual applicating and exploitation of compound traditional Chinese veterinary medicine
Lay a good foundation.
Embodiment 2:
A kind of test of pesticide effectiveness treating infectious bronchitis of chicken herbal medicine microcapsule:
Respiratory disease of chicken is a kind of Acute exposure sexually transmitted disease caused by antibacterial or virus.Infectious bronchitis (IB),
Infectious laryngotracheitis (ILT), infective rhinitis (IC), fowl mycoplasma (CRD) etc. all show respiratory symptom, mainly show
Stretching neck for sick chicken to shake the head, sneeze, breathe dyspnea with rapid respiration and cacophonia, eye, nose flow liquid, spirit is tired, and loss of appetite etc., cuing open inspection can
See respiratory system in petechia in various degree or hyperemia, inflammatory symptom is obvious, and sick chicken is also often accompanied by digestive system and other organs
Such as comprehensive pathological changes such as intestinal bleeding, Liver and kidney enlargement, pericardium inflammation, the general course of disease of respiratory tract disease is longer, has a strong impact on broiler
Growth and Performance of Laying, cause heavy losses to aviculture.Make full use of the existing drug resource of China, explore and develop new drug
The respiratory tract disease such as infectious bronchitis of fowl, infectious laryngotracheitis is treated by preparation, to improve fowl culture benefit and
Animal health control effect is significant.
1 materials and methods:
1.1 test materials:
1.1.1 a kind of pharmaceutical composition microcapsule (letter below for treating infectious bronchitis of chicken that the present invention provides
Claim microcapsule of the present invention): Lanzhou Livestock and Animal Drug Inst., Chinese Academy of Agricultural Science develops, and its prescription is respectively as follows:
Microcapsule 1 of the present invention:
Herba Ephedrae extract 0.01kg, Folium Isatidis extract 0.1kg, Herba Hyperici perforati extract 0.2kg, Flos Lonicerae extract 0.1kg,
Rhizoma Coptidis extract 0.2kg.
Microcapsule 2 of the present invention:
Herba Ephedrae extract 0.05kg, Folium Isatidis extract 0.01kg, Herba Hyperici perforati extract 0.1kg, Flos Lonicerae extract
0.2kg, Rhizoma Coptidis extract 0.01kg.
Microcapsule 3 of the present invention:
Herba Ephedrae extract 0.1kg, Folium Isatidis extract 0.05kg, Herba Hyperici perforati extract 0.01kg, Flos Lonicerae extract
0.01kg, Rhizoma Coptidis extract 0.1kg.
Comparison 1: ribavirin 0.1kg.
1.1.2 test chicken 10 Japanese instar chickling 1000, purchased from Gansu China Gansu Province chicken house, tests big mountain, Lanzhou in institute and moves
Thing Experimental Base.
1.1.3 bacterium (malicious) strain haemophilus paragallinarum N strain, avian infectious bronchitis virus M41 strain, be derived from China beast
Pharmaceuticals supervise institute.
1.2 test methods:
1.2.1 raw material prepares to be prepared as described above this medicine, loads in clean turnover barrel, seals, proceeds to room to be checked and treat
Inspection.Detect qualified after, put powder automatic packer by regulation subpackage, sealing.
1.2.2 haemophilus paragallinarum is inoculated in the chicken serum chicken soup culture medium containing 2% Sanguis caprae seu ovis Lactoferrin by antibacterial culturing
In, cultivate 24 h, according to a conventional method count plate for 37 DEG C.
1.2.3 M41 strain EID50 measures kind of 100 times of dilutions of poison, is inoculated in 10 age in days chick embryo allantoic cavities, every embryo 0.1
ML, 37 DEG C of hatching 48 h, take allantoic fluid standby and observe embryonic lesions.
1.2.4 counteracting toxic substances dosimetry takes test chicken 20, random point 4 groups, respectively per nasal, eye instill IBV and secondary chicken addicted to
Blood bacillus equivalent mixed liquor 0.1,0.2,0.3,0.4 ml, observes chicken incidence after counteracting toxic substances.
1.3 microcapsule of the present invention are to respiratory disease of chicken therapeutic test:
1.3.1 test packet and process 10 age in days chicken 250, be randomly divided into 5 groups, often group 50, the respectively micro-glue of the present invention
Wafer 3,2,1 group, matched group, every chicken is instilled by the counteracting toxic substances dosage per nasal determined, eye and infects contamination.Test chicken is after counteracting toxic substances 24
H, is mixed by the microcapsule dosage of the present invention adding 2mg in per kilogram feedstuff respectively and raises administration, be used in conjunction 5d.Drug control group counteracting toxic substances
Rear 24 h are mixed by per kilogram 2mg and raise administration, and method is ibid.
1.3.2, during efficacy assessment code test, observe chicken group every day, after the course for the treatment of add up effective percentage, mortality rate and
Body weight increase rate.
1.4 microcapsule of the present invention and randomized controlled treatment Comparision Test:
1.4.1 test packet will be divided into 2 groups for examination chicken, and the 1st group is microcapsule group (2) of the present invention, 300, by 2mg/kg
Dosage mix and raise administration, be used in conjunction 5 d；Matched group (1), is mixed by the dosage of 2mg/kg and raises administration, be used in conjunction 5 d by 300.
2 results:
The therapeutic effect 37 DEG C of trainings of 2.1.1 counteracting toxic substances dosage haemophilus paragallinarum to respiratory disease of chicken of 2.1 microcapsule of the present invention
After supporting 24 h, count plate is 20 × 108 CFU/mL.The ELD50 of avian infectious bronchitis virus M41 strain is 10-6.5/
0.1 mL.Chicken 48 h after counteracting toxic substances fall ill successively, wherein all morbidities of 0.2-0.4 mL group, it is thus determined that 0.2 mL/ is only
For counteracting toxic substances dosage.
2.1.2 34-38 h after counteracting toxic substances sequela chicken symptom counteracting toxic substances, positive controls falls ill successively, performance dyspnea,
Shed tears, the symptom such as sneeze, there is death in 98h.After death cuing open the visible chicken face swelling of inspection, eye conjunctivitis, edema, nasal cavity has glutinous
Fluidity secretions, trachea has mucus exudate, and air bag is muddy.Chicken pathogen separation of dying of illness is positive.
The efficacy result of microcapsule the most of the present invention is shown in Table 8.As can be seen from Table 8, microcapsule of the present invention is respectively tested
Group chicken survival number, treated effect are above matched group, and microcapsule of the present invention 2 groups and microcapsule of the present invention 3 groups the highest
In microcapsule 1 of the present invention.Microcapsule 2 test group body weight increase rate of the present invention is higher than other groups, integrated cost and animal peace
Total factor, selection condition microcapsule of the present invention 2 groups is optimum formula.
Table 8
2.2 microcapsule 3 of the present invention compare with compareing medicine therapeutic effect:
Microcapsule 2 of the present invention is shown in Table 9 with compareing medicine therapeutic effect comparative result.From table 9, microcapsule of the present invention 2 groups
The cure rate of morbidity chicken is higher than matched group, and mortality rate notable matched group group；The average weight gain of microcapsule 2 of the present invention is with relative
Weightening finish is significantly higher than matched group.
Table 9
3 discuss:
3.1, at present by herbal medicine Study on Modernization, develop the dosage form made new advances.Also finding out from this result of the test, the present invention is micro-
The sickness rate that capsule is 1,2,3 groups is substantially reduced than the reduction of positive controls sickness rate respectively, and cure rate, effective percentage and weightening finish have
Improved.Considering No. 2 prescriptions by a large amount of clinical practices and above test is optimal prescription.
3.2 according to this result of the test, and microcapsule clinic of the present invention recommends consumption to be that 2-4 mg/kg mixes and raises administration, 5d
It it is a course for the treatment of.But in actual production, can take the circumstances into consideration increase dosage or extend the period for the treatment of according to the concrete state of an illness.
In sum, microcapsule of the present invention has good therapeutic effect to chicken chronic respiratory tract disease, and cure rate reaches
92.1%, higher than comparison, applying microcapsule the relative weight gain of the present invention is 129, and therefore, said preparation has wide clinically
Application value.
Finally it is noted that the foregoing is only the preferred embodiments of the present invention, it is not limited to the present invention,
Although being described in detail the present invention with reference to previous embodiment, for a person skilled in the art, it still may be used
So that the technical scheme described in foregoing embodiments to be modified, or wherein portion of techniques feature is carried out equivalent.
All within the spirit and principles in the present invention, any modification, equivalent substitution and improvement etc. made, should be included in the present invention's
Within protection domain.
Claims (6)
1. the pharmaceutical composition being used for treating infectious bronchitis of chicken, it is characterised in that by parts by weight
Following components prepares: Herba Ephedrae extract 0.1-1 part, Folium Isatidis extract 0.1-1 part, Herba Hyperici perforati extract 0.1-2
Part, Flos Lonicerae extract 0.1-2 part, Rhizoma Coptidis extract 0.1-2 part.
A kind of pharmaceutical composition for treating infectious bronchitis of chicken the most according to claim 1, it is characterised in that
It is to be prepared by following components in parts by weight: Herba Ephedrae extract 0.5 part, Folium Isatidis extract 0.1 part, pass through leaf
Fructus Forsythiae extract 1 part, Flos Lonicerae extract 2 parts, Rhizoma Coptidis extract 0.1 part.
A kind of pharmaceutical composition for treating infectious bronchitis of chicken the most according to claim 1 and 2, its feature exists
In, the dosage form of described pharmaceutical composition is microcapsule and any one dosage form conventional.
The system of a kind of pharmaceutical composition microcapsule for treating infectious bronchitis of chicken the most according to claim 3
Preparation Method, it is characterised in that weigh each component as core according to weight, add a small amount of anhydrous alcohol solution, progressively add
To as in the beta-schardinger dextrin-of wall material and the mixture of modified starch, after colloid mill grinds, at 4 DEG C, balance 12 h, reclaim second
Alcohol, i.e. obtains the pharmaceutical composition microcapsule for treating infectious bronchitis of chicken after filtration drying.
Preparation method the most according to claim 4, it is characterised in that the core content in described microcapsule is according to weight
Percentages is 10-60%；The grinding rotating speed of described colloid mill is 1200r/min, and grinding temperature is 20 DEG C-60 DEG C, colloid barreling
Time consuming is 10-60min；The mass ratio of described beta-schardinger dextrin-and modified starch is (1-6): (1-6).
Preparation method the most according to claim 5, it is characterised in that the core content in described microcapsule is according to weight
Percentages is 30%；The grinding temperature of described colloid mill is 30 DEG C, and colloid mill milling time is 30min；Described beta-schardinger dextrin-and
The mass ratio of modified starch is 4:3.
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Cited By (1)
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| CN108888690A (en) * | 2018-06-29 | 2018-11-27 | 广西万寿谷投资集团股份有限公司 | A kind of feed addictive and preparation method thereof for preventing and treating infectious bronchitis |
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