CN102274303A - Traditional Chinese medicine extract compound injection for treating yellow-white dysentery of piglets and preparation method - Google Patents
Traditional Chinese medicine extract compound injection for treating yellow-white dysentery of piglets and preparation method Download PDFInfo
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Abstract
The invention relates to a traditional Chinese medicine extract compound injection for treating yellow-white dysentery of piglets, each 100ml of injection liquid of the injection comprises the following raw materials and auxiliary materials: 10-50mg of berberine sulphate with the purity of not less than 98%; 50-200mg of scutelloside with the purity of not less than 98%; 250-400mg of 30:1 Chinese pulsatilla root extract; 250-400mg of 30:1 ash bark extract; 250-400mg of 50:1 purpleflower holly leaf extract; 250-500mg of 30:1 copperleaf herb extract; 250-500mg of 20:1 lightyellow sophora root extract; 250-500mg of 20:1 common andrographis herb extract; 0.75ml of Tween-80; and 100mg of sodium sulfite. The traditional Chinese medicine extract compound injection adopts Chinese herbal medicines, namely golden thread, baical skullcap root, Chinese pulsatilla root, the ash bark, the copperleaf herb, the purpleflower holly leaf, the lightyellow sophora root and the common andrographis herb, with the effects of stopping diarrhea and reducing heat, active ingredients in all the medicines in the formula are extracted according to the properties of the active ingredients for preparing the compound injection which is in line with requirements of pharmaceutical pharmacy, and the injection adopts the combination of pure traditional Chinese medicines, has no pollution and is convenient for administration and very significant in efficacy.
Description
Technical field
The present invention relates to a kind of veterinary drug preparation for the treatment of yellow and white dysentery of piglet, relate in particular to a kind of Chinese medicine extract compound injection and preparation method for the treatment of yellow and white dysentery of piglet.
Background technology
Yellow scour of piglet, Hakuri are the multiple infectious intestinal diseases of the piglet of sucking the breast that is caused by dust Xi Shi escherichia coli, the general designation colibacillosis, this disease distributes very wide, and all there is generation in the place of all foster mother pigs, be cause the piglet incubation rate low, influence one of main infectious disease of pig industry economic benefit.Wherein yellow scour of piglet is a kind of acute infectious intestinal disease that newborn piglet is often sent out, and mainly betides 7 ages in days with interior piglet, and M ﹠ M is all very high, because morbidity is anxious, the course of disease is short, age in days is little, brings very big difficulty for treatment work.Piglet pujos blancos mainly betides 14～30 age in days piglets, the sickness rate height, and mortality rate is low, through treatment in good time, though major part all can be cured, hinders growth in piglets to a certain extent and grows.
At present, the treatment of this disease is mainly based on various antibacterials, as penicillin, oxytetracycline, chloromycetin, quinolinones etc., but because antibiotic prolonged and repeated use and abuse, cause this pathogenic microorganism to having produced drug resistance, and drug residue influenced the quality of livestock products, and the normal exit trade to animal product causes very big obstacle on the one hand, also brings harm to the consumer health on the other hand.Therefore, searching nothing (low) medicine is residual, nothing (low) is polluted, can be substituted the research of antibiotic growth promoting substance as emphasis.The less generation drug resistance of Chinese herbal medicine life-time service is not prone to residual, Drug resistance, toxic and side effects, has safety and reliability, is that other antibiotic is unrivaled.
For many years, some veterinary work persons make great efforts to explore on forefathers' experiential basis, are carrying out good try aspect herbal control piglet Huang, Hakuri.Report dysentery relieving soup such as Zhang Jingli, pulvis curcumae, Radix Pulsatillae Decoction have prevention and therapeutical effect preferably to colibacillosis of piglet, to the cure rate of yellow scour of piglet more than 93%. effective percentage 100%; To piglet from the cure rate of dysentery disease 100%.Qin Sihai report, the Chinese herb compound of being made up of the Radix Pulsatillae, Cortex Phellodendri, Cortex Fraxini, Rhizoma Coptidis, the Radix Astragali, Radix Angelicae Sinensis, Semen Castaneae male flower wadding etc. of plan certainly has treats the yellow scour of piglet effect preferably.The Chen Jinchan test shows the concentrated solution of making every milliliter of 1g crude drug with Radix Pulsatillae 12g, Radix Gentianae 6g, Rhizoma Coptidis 5g, brown sugar 3g, gavage warm in advance good Chinese medicine concentrated solution 3mL with every piglet, every day 2 times, the dosage of logotype 3d, treatment to yellow scour of piglet has good effect, and cure rate is up to 93.9%.The Wang Ziran report can obtain curative effect (cure rate 83.3%, clinical cure rate 86.9% manually cause a disease) preferably when intending Chinese herb compound at the treatment yellow scour of piglet, suitable with the antibiotic Western medicine of sensitivity, and uses still safer by 8 multiple doses.The Li Yiduan report, Chinese medicine " spirit of diarrhoea potion " has better therapeutic effect to diarrhea of pigs, and average cure rate reaches 96.6%.Open team of four horses's dawn etc. and add Chinese herbal medicine extract " precious I number of joy " in the suckling piglet daily ration, can prevent suckling piglet diarrhoea preferably, especially piglet between weaning stage, its weightening finish is better than antibiotic with anti-diarrhoea effect.
Yang Hongbin etc. have reported the clinical experimental study of the husky Islam Ghusl of novel compound Chinese medicine preparation (injection injection and oral liquid) to treatment piglet Huang, Hakuri, its medicine component comprises that natural Chinese medicines such as Herba Andrographis, Semen Lablab Album, Radix Codonopsis, Rhizoma Atractylodis, Cortex Fraxini are raw material, the result shows: husky big " Cuochuang jin " oral liquor group to the cure rate of yellow scours be 83%, effective percentage is 92%,, the cure rate of Hakuri is 88%, effective percentage is 95%; Husky Islam Ghusl injection group to the cure rate of yellow scours be 92%, effective percentage is 96%, to the cure rate of Hakuri be 93%, effective percentage is 98%.Zhao Hengzhang etc. have reported that herbal injection studies the colibacillosis of piglet therapeutic effect, herbal injection is developed by colibacillosis seminar of Chinese veterinarian teaching and research room of Henan Vocation Technology Normal Inst., mainly form by 11 flavor Chinese medicines such as Gypsum Fibrosum, Talcum, the Radix Pulsatillae, Rhizoma Atractylodis, it is to the high curative rate 97.6% of yellow and white dysentery of piglet, average out to 97.3%.Sun Zhi very waits and has studied compound recipe Herba Solani Lyrati injection to colibacillary external bacteriostasis with to the therapeutic effect of colibacillosis of pigs.The little clear grade of Guo has been reported the clinical efficacy test of Chinese herb prevention yellow and white dysentery of piglet, wherein can to pacify the injection main component be 1% berberine sulfate etc. to dysentery, the cure rate of its high, medium and low dosage treatment yellow and white dysentery of piglet is respectively 93.02%, 91.67%, 80.00%, and effective percentage is respectively 97.67%, 94.44%, 86.67%.The loyalty Tang Dynasty etc. has reported that the young dysentery of Chinese medicine stops the therapeutic test to yellow scour of piglet and Hakuri, wherein the young dysentery of Chinese medicine medicine stop be with the Radix Pulsatillae, Radix Codonopsis, Rhizoma Zingiberis, Cortex Phellodendri, Radix Scutellariae, Radix Paeoniae Rubra, the Radix Paeoniae Alba, Cortex Fraxini, Radix Curcumae, Herba Artemisiae Scopariae, Fructus Hordei Germinatus, Herba Pogostemonis in 3: 2: 1: 3: 2: 1: 1: 2: 1: 1: 1: 2 ratio prescription, mix, pulverize, sieve, the spice sow of feeding, the result shows, the Chinese drug-treated group cure rate is 88.5%, and effective percentage is 100%.10 Chinese medicine medicines such as the employing Radix Pulsatillae, Cortex Phellodendri, Cortex Fraxini, Radix Gentianae such as Huang Meng are made powder, piglet diarrhea diseases such as treatment yellow and white dysentery of piglet, and cure rate reaches 94.5%.Zhu Zhaorong etc. filter out the oral liquid gram dysentery king that Chinese herbal medicine such as the Radix Pulsatillae, the Radix Astragali and Radix Glycyrrhizae are made " rheocamphoradin " treatment yellow and white dysentery of piglet, make western medicine group with norfloxacin and gentamycin, and the result shows that it is 94.01% and 96.34% that gram dysentery king organizes cure rate.Li Wenxiong etc. have reported the prevention and treatment in Chinese and western yellow and white dysentery of piglet, and Chinese medicinal formulae is prescription Radix Pulsatillae 20g, Rhizoma Coptidis 10g, Cortex Phellodendri 10g, Radix Sophorae Flavescentis 10g, Radix Gentianae 5g, Herba Andrographis 15g, Flos Lonicerae 10g, and administering mode is for mixing food.Yellow product Lu etc. has reported the Chinese herbal treatment yellow and white dysentery of piglet, adopts Herba Acalyphae 90g, Ramulus et Folium Bauhiniae Faberi 60g to fry in shallow oil juice and gavage.Wu Junwei etc. apply for a patent " a kind of Chinese traditional compound medicine that is used for the treatment of yellow and white dysentery of piglet ", number of patent application 200710093250.4, disclosure of the Invention a kind of Chinese traditional compound medicine-Jin Gen injection that is used for the treatment of yellow and white dysentery of piglet, it is to distinguish the flavor of medical materials through the modern Chinese medicine extraction process by Flos Lonicerae and Radix Isatidis two, its refining effective ingredients of extraction separation forms, be used for the treatment of yellow and white dysentery of piglet and can not produce drug resistance, and drug residue free, have no side effect.Also had many bibliographical informations in addition and fed the Chinese prescription for the treatment of yellow and white dysentery of piglet, wherein contained Radix Scutellariae, the Radix Pulsatillae, Cortex Fraxini, Herba Acalyphae, Radix Sophorae Flavescentis and Herba Andrographis respectively.
Though the herbal medicine of China research and development have obtained certain progress, and within the specific limits the control of livestock and poultry is obtained effect preferably, accumulated abundant clinical experience.And has certain theoretical basis.Chinese herbal medicine not only can be formed compound recipe, but also folk prescription uses.From the complexity of present swine diseases, Chinese herbal medicine highlights all the more in the advantage of swine diseases aspect preventing and treating, especially aspect the treatment and prevention and food safety of mixed infection.Along with the development of Chinese herbal and crude drugs preparations technology, as traditional and classical swine diseases means of prevention, Chinese herbal medicine will yield unusually brilliant results.At present, China has had multiple product to occur. and some Chinese herbal compound preparations have been brought into play important function aspect the preventing and treating of pig disease.The therapeutic effect that has is suitable with some antibiotic, and the effect that has also is better than antibiotic.Therefore, carrying out the not still requirement of sustainable animal husbandry development of herbal control swine diseases, also is that people are to the requirement of improving the quality of living and pollution-free food is consumed.When obtaining effect, also there are some problems.
In general, quantity is many, level is low, lack the large-scale production and the capability of independent innovation, research and development expense insufficient investment, the experience of shortage market economy and international and domestic standard aspect.Especially big, the effective ingredient of addition is not clear, the mechanism of action is unclear, action effect is unstable, dosage form is single, and peroral dosage form on the high side and research, raw material and quality control standard injection type shortage toxicity on the low side secure context are incomplete etc.Chinese medicine preparation among " People's Republic of China's veterinary drug standard " (version in 1992, two ones) is totally 154 sides, and wherein 128 is powder, accounts for 82.4%, and all the other are fluid extract, unguentum, tincture, injection etc.Although provincial standard also has some other dosage forms, limited amount, qualified is few.So not only do not satisfy the needs of domestic and international market. let alone the similar advanced product with the world of going abroad contends with.In view of above problem, be badly in need of development safety, efficiently, new drug easily.
Summary of the invention
Technical problem to be solved by this invention provides the Chinese medicine extract compound injection of the treatment yellow and white dysentery of piglet of a kind of low toxicity, efficient, noresidue.
Another technical problem to be solved by this invention provides the preparation method of this compound injection.
For addressing the above problem, a kind of Chinese medicine extract compound injection for the treatment of yellow and white dysentery of piglet of the present invention is characterized in that: comprise following supplementary material in the every 100mL injection of this injection:
Baicalin 50～the 200mg of berberine sulphate 10～50mg purity 〉=98% of purity 〉=98%
30: 1 Cortex Fraxini extract 250～400mg of 30: 1 Radix Pulsatillae extract 250～400mg
30: 1 Herba Acalyphae extracts of 50: 1 SIJIQING extract 250～400mg, 250～500mg
20: 1 Herba Andrographis extract 250～500mg of 20: 1 Radix Sophorae Flavescentis extract 250～500mg
Tween 80 0.75mL sodium sulfite 100mg.
This injection is the water needle injection of pH=7.5.
Aforesaid a kind of preparation method for the treatment of the Chinese medicine extract compound injection of yellow and white dysentery of piglet may further comprise the steps:
(1) by each component of proportioning weighing;
(2) baicalin, sulphuric acid berberine are dissolved in respectively in the water for injection of 20mL, transfer pH to 7.0～7.5, and make described baicalin and the dissolving of sulphuric acid berberine, obtain baicalin solution and sulphuric acid berberine solution respectively with sodium hydroxide;
(3) with after described baicalin solution and the mixing of sulphuric acid berberine solution, add 50mL water for injection, add 30: 1 Radix Pulsatillae extracts, 30: 1 Cortex Fraxini extract, 50: 1 SIJIQING extract, 30: 1 Herba Acalyphae extracts, 20: 1 Radix Sophorae Flavescentis extracts, 20: 1 Herba Andrographis extracts more successively, make its dissolving, obtain mixture solution;
(4) in described mixture solution, add 1～2g active carbon, boiled 10～15 minutes, behind the natural cooling,, obtain filtrate through centrifugal, sucking filtration;
(5) in described filtrate, add tween 80, sodium sulfite successively, add 90～95mL water for injection behind the mix homogeneously, and, be settled to 100mL then with sodium hydroxide adjust pH to 7.5; Be sub-packed at last in the cillin bottle, behind the boiling water bath heat sterilization, promptly get water needle injection.
The preparation method of described 30: 1 Radix Pulsatillae extracts may further comprise the steps:
(1) root of the Radix Pulsatillae is pulverized the back and cross 40～80 mesh sieves, obtain Radix Pulsatillae powder;
(2) in described Radix Pulsatillae powder, add the distilled water of 7 times of its quality, transfer pH to 10, under 80 ℃ of temperature, soak into the 4h after-filtration, obtain filtrate A and precipitate A with sodium hydroxide;
(3) in described precipitate A, add the distilled water of 5 times of its quality, transfer pH to 10, under 80 ℃ of temperature, soak into the 2h after-filtration, obtain liquor B and sediment B with sodium hydroxide;
(4) merge described filtrate A and described liquor B, and transfer pH to 7, behind concentrating under reduced pressure, obtain concentrated solution with sodium hydroxide;
(5) extraordinarily to go into mass concentration by 3 of its volume be 95% ethanol to described concentrated solution, and behind the precipitation 24h, centrifugal 10min obtains supernatant;
(6) described supernatant after concentrating under reduced pressure, drying, promptly gets the Radix Pulsatillae extract of paste successively.
The preparation method of described 30: 1 Cortex Fraxini extract may further comprise the steps:
(1) Cortex Fraxini is pulverized the back and cross 40～80 mesh sieves, obtain the Cortex Fraxini powder;
(2) extraordinarily going into mass concentration by 3 of its quality in described Cortex Fraxini powder is 95% ethanol, and the 6h that refluxes in boiling water bath behind decompression recycling ethanol, obtains concentrating medicine;
(3) described concentrated medicine behind vacuum drying, promptly gets the Cortex Fraxini extract of paste by 1～2 distilled water precipitation extraordinarily of its quality.
The preparation method of described 50: 1 SIJIQING extract may further comprise the steps:
(1) Ilex purpurea Hassk.[I.chinensis Sims is pulverized the back and cross 40～80 mesh sieves, obtain four seasons Calomelas;
(2) with 8～10 extraordinarily distilled water of described four seasons Calomelas, under 100 ℃ of temperature, decoct 3 times, boiled 1 hour at every turn by its quality, after the filtration, merging filtrate;
(3) described filtrate obtains concentrated solution behind concentrating under reduced pressure; It is 95% ethanol that this concentrated solution is extraordinarily gone into mass concentration by 8～10 of its volume, stirs evenly, and places after 3～5 days, reclaims ethanol, obtains supernatant;
(4) described supernatant is successively after being evaporated to syrupy shape, is that merge extractive liquid, is extracted in 95% ethanol gradation with mass concentration; Described extracting solution was placed 1 day, filtered decompression recycling ethanol, promptly got melicera SIJIQING extract.
The Herba Acalyphae preparation method of extract may further comprise the steps in described 30: 1:
(1) the dry herb of Herba Acalyphae is pulverized the back and cross 40～80 mesh sieves, obtain the Herba Acalyphae vegetable powder;
(2) in described Herba Acalyphae vegetable powder, extraordinarily go into distilled water, under 100 ℃ of temperature, decoct 2～3 times, boiled 1 hour at every turn by 10 of its quality, after the filtration, merging filtrate;
(3) described filtrate promptly gets the Herba Acalyphae extract after concentrating under reduced pressure, drying.
The preparation method of described 20: 1 Radix Sophorae Flavescentis extracts may further comprise the steps:
(1) Radix Sophorae Flavescentis is pulverized the back and cross 40～80 mesh sieves, obtain Radix Sophorae Flavescentis powder;
(2) adding 7 times of its quality, mass concentration in described Radix Sophorae Flavescentis powder is 95% ethanol, and backflow 6h obtains filtrate A and precipitate A;
(3) adding 5 times of its quality, mass concentration in described precipitate A is 95% ethanol, and backflow 4h obtains liquor B and sediment B;
(4) merge described filtrate A and described liquor B, after concentrating under reduced pressure reclaims ethanol, obtain concentrated solution; After this concentrated solution drying, promptly get the Radix Sophorae Flavescentis extract of paste.
The preparation method of described 20: 1 Herba Andrographis extracts may further comprise the steps:
(1) Herba Andrographis is pulverized the back and cross 40～80 mesh sieves, obtain the punching Nelumbo nucifera Gaertn. Powder;
(2) in described punching Nelumbo nucifera Gaertn. Powder, add the distilled water of 7 times of its quality, under 80 ℃ of temperature, soak into the 4h after-filtration, obtain filtrate A and precipitate A;
(3) in described precipitate A, add the distilled water of 5 times of its quality, under 80 ℃ of temperature, soak into the 2h after-filtration, obtain liquor B and sediment B;
(4) merge described filtrate A and described liquor B, behind concentrating under reduced pressure, obtain concentrated solution;
(5) extraordinarily to go into mass concentration by 3 of its volume be 95% ethanol to described concentrated solution, and behind the precipitation 24h, centrifugal 10min obtains supernatant;
(6) described supernatant after concentrating under reduced pressure, drying, promptly gets the Herba Andrographis extract of paste successively.
The present invention compared with prior art has the following advantages:
1, the present invention adopts Chinese herbal medicine Rhizoma Coptidis, Radix Scutellariae, the Radix Pulsatillae, Cortex Fraxini, Herba Acalyphae, Ilex purpurea Hassk.[I.chinensis Sims, Radix Sophorae Flavescentis and the Herba Andrographis with antidiarrheal, antipyretic effect, character according to its effective ingredient, each pharmaceutically active ingredient extracts to writing out a prescription, extracorporeal bacteria inhibitor test and prescription by single Chinese herbal medicine effective extract carry out extracorporeal bacteria inhibitor test, filter out the optimum formula of control yellow and white dysentery of piglet, the amount of extract is through decolouring, filtration, degerming, interpolation stabilizing agent, require to be prepared into compound injection according to pharmaceutics of Chinese drugs, as the novel formulation of treatment yellow and white dysentery of piglet.By outward appearance and physicochemical property, aseptic, mucous membrane irritation, muscular irritation, haemolysis and pyrogen test, show that injection meets the galenic pharmacy requirement.
2, the present invention carries out external bacteriostatic activity research to each effective component extracts to Escherichia coli, the pure product of berberine sulphate, the pure product of baicalin, 30: 1 Radix Pulsatillae extracts, 30: 1 Cortex Fraxini extract, 50: 1 Ilex purpurea Hassk.[I.chinensis Sims, 30: 1 Herba Acalyphae extracts, 20: 1 Radix Sophorae Flavescentis extracts and 20: 1 Herba Andrographis extracts all have fungistatic effect preferably to the piglet Escherichia coli as a result, and wherein the external fungistatic effect of pure product of berberine sulphate and the pure product of baicalin is the most remarkable.The results are shown in Table 1.
Eight kinds of Chinese herbal medicine extracts of table 1 are to eight kinds of colibacillary MIC measurement results (mg/ml)
The pure product of berberine sulphate, the pure product of baicalin, 30: 1 Radix Pulsatillae extracts, 30: 1 Cortex Fraxini extract, 50: 1 Ilex purpurea Hassk.[I.chinensis Sims, 30: 1 Herba Acalyphae extracts, 20: 1 Radix Sophorae Flavescentis extracts and 20: 1 Herba Andrographis extracts are carried out component (referring to table 2) according to the preliminary experiment result according to certain ratio, and carry out extracorporeal bacteria inhibitor test according to agar plate dilution method, fungistatic effect significantly (referring to table 3) as a result.
Table 2 Chinese medicine extraction composition formula is formed (unit: g/ml)
Table 3 compound injection minimal inhibitory concentration is measured
| Consumption | The result |
| 1ml | All bacterium are all suppressed |
| 0.8ml | All bacterium are all suppressed |
| 0.6ml | All bacterium are all suppressed |
| 0.5ml | All bacterium are all suppressed |
| 0.4ml | All bacterium are all suppressed |
| 0.3ml | All bacterium are all suppressed |
| 0.2ml | O2 does not suppress, and all the other all suppress |
| 0.1ml | All can not suppress |
Annotate: extract is drawn corresponding amount, and the agar culture medium that is added to final volume 10mL carries out the agar plate bacteriostatic test.
3, quality inspection:
Requirement according to " Chinese medicine, natural drug stability test technological guidance principle " for animals, " Chinese medicine, natural drug local irritation and hemolytic investigative technique guideline ", " technological guidance's principle of Chinese medicine, natural drug studies on acute toxicity " and " Chinese veterinary drug allusion quotation " 2005 editions is carried out quality inspection to the present invention.By outward appearance and physicochemical property, aseptic, mucous membrane irritation, muscular irritation, haemolysis and pyrogen test experiment, the result shows that every loading amount of this injection is no less than its labelled amount; No visible haze and insoluble matter; Stable in properties, safety meet the galenic pharmacy requirement.Injection has been carried out stability (quickening and long term test) test, and the result adds 0.75% tween 80 can make injection preserve 1 year at ambient-temp-stable as solubilizing agent, 0.1% sodium sulfite as antioxidant; Adopt improvement karber's method lumbar injection Kunming white mice to measure the LD50 of compound injection, LD50 is 5260.17mg/kg as a result.95% of LD50 average crediblely is limited to 5260.17 ± 650.95, can be defined as actual nontoxic according to the Chinese materia medica tocixity grade scale.
Therefore, prepared injection meets the galenic pharmacy requirement.
4, compound injection of the present invention is to the therapeutic effect of yellow and white dysentery of piglet:
Select 5～20 ages in days not have 80 of medical history, non-immune piglets, escherichia coli K99 broth cultivation bacteria liquid is gavaged by every pig 10,000,000,000 viable bacteria oral cavities, carry out artificial vaccination.Inoculation back 24h gathers feces with aseptic cotton carrier at rectum, and K99 does agglutination test with the escherichia coli standard serum, to confirm to have or not infection due to Escherichia coli.
Test pig is divided into 4 groups at random, and preceding 3 groups is test group, and the 4th group is matched group.Isolated rearing, regularly drink water every day, treats after piglet is suffered from diarrhoea.1st, 2,3 groups of respectively intramuscular injection compound injection 0.25ml/kg of the present invention, 0.5ml/kg, 1ml/kg, 2 times/d, logotype 3 days; Matched group intramuscular injection sterile saline, 2ml/, 2 times/d.All test piglets are all under equal conditions raised.
Duration of test is observed and respectively organize pig spirit, feces, appetite and piglet death condition every day.After medication in 3 days treatment, diarrhoea amount or diarrhoea number of times reduce, disappear, and feces is shaped, and it is normal that spiritual appetite is recovered, and is considered as curing; After treatment in 3 days, diarrhoea does not stop, and feces is rarer, and spiritual diet is not very good, is considered as effectively.The results are shown in Table 4.
Table 4 compound injection is to the therapeutic effect of yellow and white dysentery of piglet
Annotate: 2ml/ normal saline of contrast injection.
As shown in Table 4, compound injection 0.25ml/kg of the present invention, 0.5ml/kg, three dosage of 1.0ml/kg all have obvious curative effects to yellow and white dysentery of piglet, artificial challenge's yellow and white dysentery of piglet cure rate is respectively 60%, 95% and 95%, and the matched group mortality rate is up to 75%; And therefore 0.5ml/kg and two dosage treatment effects of 1.0ml/kg basically identical, select the treatment consumption of 0.5ml/kg as yellow and white dysentery of piglet for use.
5, the contrast test of compound injection of the present invention and other medicines:
Select 5～20 ages in days not have 350 of medical history, non-immune piglets, escherichia coli K99 broth cultivation bacteria liquid is gavaged by every pig 10,000,000,000 viable bacteria oral cavities, carry out artificial vaccination.Inoculation back 24h gathers feces with aseptic cotton carrier at rectum, and K99 does agglutination test with the escherichia coli standard serum, to confirm to have or not infection due to Escherichia coli.
Test pig is divided into 5 groups at random, the 1st group of intramuscular injection gentamycin sulfate; The 2nd group of intramuscular injection norfloxacin injection; The 3rd group of intramuscular injection ceftiofur suspension injection; The 4th group of intramuscular injection berberine sulphate injection; The 5th group of intramuscular injection Macleaya cordata injection; The 6th group of intramuscular injection compound injection; The 7th group is matched group, intramuscular injection sterile saline, 2ml/, 2 times/d.Below respectively organize isolated rearing, regularly drink water every day, and all test piglets are all under equal conditions raised.
Duration of test is observed and respectively organize pig spirit, feces, appetite and piglet death condition every day.After medication in 3 days treatment, diarrhoea amount or diarrhoea number of times reduce, disappear, and feces is shaped, and it is normal that spiritual appetite is recovered, and is considered as curing; After treatment in 3 days, diarrhoea does not stop, and feces is rarer, and spiritual diet is not very good, is considered as effectively.The results are shown in Table 5.
Table 5 compound injection and other medicines are to the curative effect contrast test of yellow and white dysentery of piglet
The result shows by table 5, the therapeutic effect of compound injection of the present invention is best, cure rate reaches 98%, obviously be better than two kinds of herbal injections of Herba Macleayae Cordatae and berberine sulfate, also be better than ceftiofur suspension injection, gentamicin injection liquid and norfloxacin injection, therefore, compound injection of the present invention has excellent curative to yellow and white dysentery of piglet.
6, compound injection reasonable recipe of the present invention, pure Chinese medicine composition is pollution-free, convenient drug administration, curative effect highly significant.Use compound injection treatment yellow scour of piglet of the present invention, Hakuri 3100 nests on Pingliang, Gansu, Yuzhong and other places, totally 30121 sick pigs, the yellow and white dysentery of piglet cure rate reaches 98.09%, and effective percentage reaches 98.9%.
The specific embodiment
1 one kinds of Chinese medicine extract compound injections for the treatment of yellow and white dysentery of piglet of embodiment, this injection is the water needle injection of pH=7.5, comprises following supplementary material in every 100mL injection:
The baicalin 50mg of the berberine sulphate 10mg purity 98% of purity 〉=98%
30: 1 Cortex Fraxini extract 250mg of 30: 1 Radix Pulsatillae extract 250mg
30: 1 Herba Acalyphae extracts of 50: 1 SIJIQING extract 250mg 250mg
20: 1 Herba Andrographis extract 250mg of 20: 1 Radix Sophorae Flavescentis extract 250mg
Tween 80 0.75mL sodium sulfite 100mg.
The preparation method of this compound injection may further comprise the steps:
(1) by each component of proportioning weighing;
(2) baicalin, sulphuric acid berberine are dissolved in respectively in the water for injection of 20mL, transfer pH to 7.0～7.5, and make described baicalin and the dissolving of sulphuric acid berberine, obtain baicalin solution and sulphuric acid berberine solution respectively with sodium hydroxide;
(3) with after described baicalin solution and the mixing of sulphuric acid berberine solution, add 50mL water for injection, add 30: 1 Radix Pulsatillae extracts, 30: 1 Cortex Fraxini extract, 50: 1 SIJIQING extract, 30: 1 Herba Acalyphae extracts, 20: 1 Radix Sophorae Flavescentis extracts, 20: 1 Herba Andrographis extracts more successively, make its dissolving, obtain mixture solution;
(4) in described mixture solution, add 1～2g active carbon, boiled 10～15 minutes, behind the natural cooling,, obtain filtrate through centrifugal, sucking filtration;
(5) in described filtrate, add tween 80, sodium sulfite successively, add 90～95mL water for injection behind the mix homogeneously, and, be settled to 100mL then with sodium hydroxide adjust pH to 7.5; Be sub-packed at last in the cillin bottle, behind the boiling water bath heat sterilization, promptly get water needle injection.
2 one kinds of Chinese medicine extract compound injections for the treatment of yellow and white dysentery of piglet of embodiment, this injection is the water needle injection of pH=7.5, comprises following supplementary material in every 100mL injection:
The baicalin 200mg of berberine sulphate 50mg purity 〉=98% of purity 〉=98%
30: 1 Cortex Fraxini extract 400mg of 30: 1 Radix Pulsatillae extract 400mg
30: 1 Herba Acalyphae extracts of 50: 1 SIJIQING extract 400mg 500mg
20: 1 Herba Andrographis extract 500mg of 20: 1 Radix Sophorae Flavescentis extract 500mg
Tween 80 0.75mL sodium sulfite 100mg.
The preparation method of this compound injection is with embodiment 1.
3 one kinds of Chinese medicine extract compound injections for the treatment of yellow and white dysentery of piglet of embodiment, this injection is the water needle injection of pH=7.5, comprises following supplementary material in every 100mL injection:
The baicalin 125mg of berberine sulphate 30mg purity 〉=98% of purity 〉=98%
30: 1 Cortex Fraxini extract 325mg of 30: 1 Radix Pulsatillae extract 325mg
30: 1 Herba Acalyphae extracts of 50: 1 SIJIQING extract 325mg 375mg
20: 1 Herba Andrographis extract 375mg of 20: 1 Radix Sophorae Flavescentis extract 375mg
Tween 80 0.75mL sodium sulfite 100mg.
The preparation method of this compound injection is with embodiment 1.
4 one kinds of Chinese medicine extract compound injections for the treatment of yellow and white dysentery of piglet of embodiment, this injection is the water needle injection of pH=7.5, comprises following supplementary material in every 100mL injection:
The baicalin 100mg of berberine sulphate 20mg purity 〉=98% of purity 〉=98%
30: 1 Cortex Fraxini extract 300mg of 30: 1 Radix Pulsatillae extract 300mg
30: 1 Herba Acalyphae extracts of 50: 1 SIJIQING extract 300mg 400mg
20: 1 Herba Andrographis extract 400mg of 20: 1 Radix Sophorae Flavescentis extract 400mg
Tween 80 0.75mL sodium sulfite 100mg.
The preparation method of this compound injection is with embodiment 1.
5 one kinds of Chinese medicine extract compound injections for the treatment of yellow and white dysentery of piglet of embodiment, this injection is the water needle injection of pH=7.5, comprises following supplementary material in every 100mL injection:
The baicalin 150mg of berberine sulphate 4mg purity 〉=98% of purity 〉=98%
30: 1 Cortex Fraxini extract 350mg of 30: 1 Radix Pulsatillae extract 350mg
30: 1 Herba Acalyphae extracts of 50: 1 SIJIQING extract 350mg 450mg
20: 1 Herba Andrographis extract 450mg of 20: 1 Radix Sophorae Flavescentis extract 450mg
Tween 80 0.75mL sodium sulfite 100mg.
The preparation method of this compound injection is with embodiment 1.
The berberine sulphate of the foregoing description 1～5 moderate purity 〉=98%, the baicalin of purity 〉=98% are produced by the strong good fortune plant material company limited in ShenFang,SiChuan city.
The preparation method of 30: 1 Radix Pulsatillae extracts may further comprise the steps:
(1) root of the Radix Pulsatillae is pulverized the back and cross 40～80 mesh sieves, obtain Radix Pulsatillae powder.
(2) in Radix Pulsatillae powder, add the distilled water of 7 times of its quality, transfer pH to 10, under 80 ℃ of temperature, soak into the 4h after-filtration, obtain filtrate A and precipitate A with sodium hydroxide.
(3) in precipitate A, add the distilled water of 5 times of its quality, transfer pH to 10, under 80 ℃ of temperature, soak into the 2h after-filtration, obtain liquor B and sediment B with sodium hydroxide.
(4) merging filtrate A and liquor B, and transfer pH to 7 with sodium hydroxide are 80～90 ℃ in temperature, vacuum is-0.08～-condition of 0.06MPa under behind the concentrating under reduced pressure, obtain concentrated solution.
(5) extraordinarily to go into mass concentration by 3 of its volume be 95% ethanol to concentrated solution, behind the precipitation 24h, with the centrifugal 10min of the speed of 3000rPm, obtains supernatant.
(6) supernatant is 80～90 ℃ in temperature, vacuum is-0.08～-condition of 0.06MPa under behind the concentrating under reduced pressure, promptly get the Radix Pulsatillae extract of paste.
The preparation method of 30: 1 Cortex Fraxini extract may further comprise the steps:
(1) Cortex Fraxini is pulverized the back and cross 40～80 mesh sieves, obtain the Cortex Fraxini powder.
(2) extraordinarily going into mass concentration by 3 of its quality in the Cortex Fraxini powder is 95% ethanol, and the 6h that refluxes in boiling water bath is 80～90 ℃ in temperature, vacuum is-0.08～-condition of 0.06MPa under behind the decompression recycling ethanol, obtain concentrating medicine.
(3) concentrate 1～2 extraordinarily distilled water precipitation of medicine by its quality, be-0.08 in vacuum～-0.06MPa, temperature is under 80 ℃ the condition behind the vacuum drying, promptly gets the Cortex Fraxini extract of paste.
The preparation method of 50: 1 SIJIQING extract may further comprise the steps:
(1) Ilex purpurea Hassk.[I.chinensis Sims is pulverized the back and cross 40～80 mesh sieves, obtain four seasons Calomelas.
(2) with 8～10 extraordinarily distilled water of four seasons Calomelas, under 100 ℃ of temperature, decoct 3 times, boiled 1 hour at every turn by its quality, after the filtration, merging filtrate.
(3) filtrate is 80～90 ℃ in temperature, vacuum is-0.08～-condition of 0.06MPa under behind the concentrating under reduced pressure, obtain concentrated solution; It is 95% ethanol that this concentrated solution is extraordinarily gone into mass concentration by 8～10 of its volume, stirs evenly, and places after 3～5 days, and ethanol is reclaimed in distillation in the boiling water bath, obtains supernatant.
(4) supernatant is 80～90 ℃ in temperature, vacuum is-0.08～-be evaporated to syrupy shape under the condition of 0.06MPa after, be that merge extractive liquid, is extracted in 95% ethanol gradation with mass concentration; Described extracting solution was placed 1 day, filtered decompression recycling ethanol, promptly got melicera SIJIQING extract.
The Herba Acalyphae preparation method of extract may further comprise the steps in 30: 1:
(1) the dry herb of Herba Acalyphae is pulverized the back and cross 40～80 mesh sieves, obtain the Herba Acalyphae vegetable powder.
(2) in the Herba Acalyphae vegetable powder, extraordinarily go into distilled water, under 100 ℃ of temperature, decoct 2～3 times, boiled 1 hour at every turn by 10 of its quality, after the filtration, merging filtrate.
(3) filtrate is 80～90 ℃ in temperature, vacuum is-0.08～-condition of 0.06MPa under behind the concentrating under reduced pressure, promptly get the Herba Acalyphae extract.
The preparation method of 20: 1 Radix Sophorae Flavescentis extracts may further comprise the steps:
(1) Radix Sophorae Flavescentis is pulverized the back and cross 40～80 mesh sieves, obtain Radix Sophorae Flavescentis powder.
(2) adding 7 times of its quality, mass concentration in Radix Sophorae Flavescentis powder is 95% ethanol, and backflow 6h obtains filtrate A and precipitate A.
(3) adding 5 times of its quality, mass concentration in precipitate A is 95% ethanol, and backflow 4h obtains liquor B and sediment B.
(4) merging filtrate A and liquor B are 80～90 ℃ in temperature, vacuum is-0.08～-after concentrating under reduced pressure reclaims ethanol under the condition of 0.06MPa, promptly get the Radix Sophorae Flavescentis extract of paste.
The preparation method of 20: 1 Herba Andrographis extracts may further comprise the steps:
(1) Herba Andrographis is pulverized the back and cross 40～80 mesh sieves, obtain the punching Nelumbo nucifera Gaertn. Powder.
(2) in the punching Nelumbo nucifera Gaertn. Powder, add the distilled water of 7 times of its quality, under 80 ℃ of temperature, soak into the 4h after-filtration, obtain filtrate A and precipitate A.
(3) in precipitate A, add the distilled water of 5 times of its quality, under 80 ℃ of temperature, soak into the 2h after-filtration, obtain liquor B and sediment B.
(4) merging filtrate A and liquor B are 80～90 ℃ in temperature, vacuum is-0.08～-condition of 0.06MPa under behind the concentrating under reduced pressure, obtain concentrated solution.
(5) extraordinarily to go into mass concentration by 3 of its volume be 95% ethanol to concentrated solution, behind the precipitation 24h, with the centrifugal 10min of the speed of 3000rPm, obtains supernatant.
(6) supernatant is 80～90 ℃ in temperature, vacuum is-0.08～-condition of 0.06MPa under behind the concentrating under reduced pressure, promptly get the Herba Andrographis extract of paste.
Claims (9)
1. Chinese medicine extract compound injection for the treatment of yellow and white dysentery of piglet is characterized in that: comprise following supplementary material in the every 100mL injection of this injection:
Baicalin 50～the 200mg of berberine sulphate 10～50mg purity 〉=98% of purity 〉=98%
30: 1 Cortex Fraxini extract 250～400mg of 30: 1 Radix Pulsatillae extract 250～400mg
30: 1 Herba Acalyphae extracts of 50: 1 SIJIQING extract 250～400mg, 250～500mg
20: 1 Herba Andrographis extract 250～500mg of 20: 1 Radix Sophorae Flavescentis extract 250～500mg
Tween 80 0.75mL sodium sulfite 100mg.
2. a kind of Chinese medicine extract compound injection for the treatment of yellow and white dysentery of piglet as claimed in claim 1, it is characterized in that: this injection is the water needle injection of pH=7.5.
3. a kind of preparation method for the treatment of the Chinese medicine extract compound injection of yellow and white dysentery of piglet as claimed in claim 2 may further comprise the steps:
(1) by each component of proportioning weighing;
(2) baicalin, sulphuric acid berberine are dissolved in respectively in the water for injection of 20mL, transfer pH to 7.0～7.5, and make described baicalin and the dissolving of sulphuric acid berberine, obtain baicalin solution and sulphuric acid berberine solution respectively with sodium hydroxide;
(3) with after described baicalin solution and the mixing of sulphuric acid berberine solution, add 50mL water for injection, add 30: 1 Radix Pulsatillae extracts, 30: 1 Cortex Fraxini extract, 50: 1 SIJIQING extract, 30: 1 Herba Acalyphae extracts, 20: 1 Radix Sophorae Flavescentis extracts, 20: 1 Herba Andrographis extracts more successively, make its dissolving, obtain mixture solution;
(4) in described mixture solution, add 1～2g active carbon, boiled 10～15 minutes, behind the natural cooling,, obtain filtrate through centrifugal, sucking filtration;
(5) in described filtrate, add tween 80, sodium sulfite successively, add 90～95mL water for injection behind the mix homogeneously, and, be settled to 100mL then with sodium hydroxide adjust pH to 7.5; Be sub-packed at last in the cillin bottle, behind the boiling water bath heat sterilization, promptly get water needle injection.
4. as claim 1 or 3 described a kind of Chinese medicine extract compound injections for the treatment of yellow and white dysentery of piglet, it is characterized in that: the preparation method of described 30: 1 Radix Pulsatillae extracts may further comprise the steps:
(1) root of the Radix Pulsatillae is pulverized the back and cross 40～80 mesh sieves, obtain Radix Pulsatillae powder;
(2) in described Radix Pulsatillae powder, add the distilled water of 7 times of its quality, transfer pH to 10, under 80 ℃ of temperature, soak into the 4h after-filtration, obtain filtrate A and precipitate A with sodium hydroxide;
(3) in described precipitate A, add the distilled water of 5 times of its quality, transfer pH to 10, under 80 ℃ of temperature, soak into the 2h after-filtration, obtain liquor B and sediment B with sodium hydroxide;
(4) merge described filtrate A and described liquor B, and transfer pH to 7, behind concentrating under reduced pressure, obtain concentrated solution with sodium hydroxide;
(5) extraordinarily to go into mass concentration by 3 of its volume be 95% ethanol to described concentrated solution, and behind the precipitation 24h, centrifugal 10min obtains supernatant;
(6) described supernatant promptly gets the Radix Pulsatillae extract of paste behind concentrating under reduced pressure.
5. as claim 1 or 3 described a kind of Chinese medicine extract compound injections for the treatment of yellow and white dysentery of piglet, it is characterized in that: the preparation method of described 30: 1 Cortex Fraxini extract may further comprise the steps:
(1) Cortex Fraxini is pulverized the back and cross 40～80 mesh sieves, obtain the Cortex Fraxini powder;
(2) extraordinarily going into mass concentration by 3 of its quality in described Cortex Fraxini powder is 95% ethanol, and the 6h that refluxes in boiling water bath behind decompression recycling ethanol, obtains concentrating medicine;
(3) described concentrated medicine behind vacuum drying, promptly gets the Cortex Fraxini extract of paste by 1～2 distilled water precipitation extraordinarily of its quality.
6. as claim 1 or 3 described a kind of Chinese medicine extract compound injections for the treatment of yellow and white dysentery of piglet, it is characterized in that: the preparation method of described 50: 1 SIJIQING extract may further comprise the steps:
(1) Ilex purpurea Hassk.[I.chinensis Sims is pulverized the back and cross 40～80 mesh sieves, obtain four seasons Calomelas;
(2) with 8～10 extraordinarily distilled water of described four seasons Calomelas, under 100 ℃ of temperature, decoct 3 times, boiled 1 hour at every turn by its quality, after the filtration, merging filtrate;
(3) described filtrate obtains concentrated solution behind concentrating under reduced pressure; It is 95% ethanol that this concentrated solution is extraordinarily gone into mass concentration by 8～10 of its volume, stirs evenly, and places after 3～5 days, reclaims ethanol, obtains supernatant;
(4) described supernatant is successively after being evaporated to syrupy shape, is that merge extractive liquid, is extracted in 95% ethanol gradation with mass concentration; Described extracting solution was placed 1 day, filtered decompression recycling ethanol, promptly got melicera SIJIQING extract.
7. as claim 1 or 3 described a kind of Chinese medicine extract compound injections for the treatment of yellow and white dysentery of piglet, it is characterized in that: the Herba Acalyphae preparation method of extract may further comprise the steps in described 30: 1:
(1) the dry herb of Herba Acalyphae is pulverized the back and cross 40～80 mesh sieves, obtain the Herba Acalyphae vegetable powder;
(2) in described Herba Acalyphae vegetable powder, extraordinarily go into distilled water, under 100 ℃ of temperature, decoct 2～3 times, boiled 1 hour at every turn by 10 of its quality, after the filtration, merging filtrate;
(3) described filtrate promptly gets the Herba Acalyphae extract behind concentrating under reduced pressure.
8. as claim 1 or 3 described a kind of Chinese medicine extract compound injections for the treatment of yellow and white dysentery of piglet, it is characterized in that: the preparation method of described 20: 1 Radix Sophorae Flavescentis extracts may further comprise the steps:
(1) Radix Sophorae Flavescentis is pulverized the back and cross 40～80 mesh sieves, obtain Radix Sophorae Flavescentis powder;
(2) adding 7 times of its quality, mass concentration in described Radix Sophorae Flavescentis powder is 95% ethanol, and backflow 6h obtains filtrate A and precipitate A;
(3) adding 5 times of its quality, mass concentration in described precipitate A is 95% ethanol, and backflow 4h obtains liquor B and sediment B;
(4) merge described filtrate A and described liquor B, after concentrating under reduced pressure reclaims ethanol, promptly get the Radix Sophorae Flavescentis extract of paste.
9. as claim 1 or 3 described a kind of Chinese medicine extract compound injections for the treatment of yellow and white dysentery of piglet, it is characterized in that: the preparation method of described 20: 1 Herba Andrographis extracts may further comprise the steps:
(1) Herba Andrographis is pulverized the back and cross 40～80 mesh sieves, obtain the punching Nelumbo nucifera Gaertn. Powder;
(2) in described punching Nelumbo nucifera Gaertn. Powder, add the distilled water of 7 times of its quality, under 80 ℃ of temperature, soak into the 4h after-filtration, obtain filtrate A and precipitate A;
(3) in described precipitate A, add the distilled water of 5 times of its quality, under 80 ℃ of temperature, soak into the 2h after-filtration, obtain liquor B and sediment B;
(4) merge described filtrate A and described liquor B, behind concentrating under reduced pressure, obtain concentrated solution;
(5) extraordinarily to go into mass concentration by 3 of its volume be 95% ethanol to described concentrated solution, and behind the precipitation 24h, centrifugal 10min obtains supernatant;
(6) described supernatant promptly gets the Herba Andrographis extract of paste behind concentrating under reduced pressure.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104306881A (en) * | 2014-10-10 | 2015-01-28 | 天津瑞贝特科技发展有限公司 | Injection for preventing and treating poultry escherichia coli diseases and preparation method of injection |
| CN104397499A (en) * | 2014-12-24 | 2015-03-11 | 武汉华扬动物药业有限责任公司 | Feed additive for preventing or easing toxin infection of piglets at weaning period, and preparation method of feed additive |
| CN104706577A (en) * | 2013-12-11 | 2015-06-17 | 天津安达生产力促进有限公司 | Veterinary baicalin injection liquid and preparing method thereof |
| CN105250460A (en) * | 2015-10-21 | 2016-01-20 | 北京康华远景科技股份有限公司 | Drug composition for treating rabbit diarrhea and preparation methods of drug composition |
| CN113855768A (en) * | 2021-11-22 | 2021-12-31 | 河南宝威生物工程技术有限公司 | Traditional Chinese medicine composition for preventing piglet diarrhea and preparation method and application thereof |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104706577A (en) * | 2013-12-11 | 2015-06-17 | 天津安达生产力促进有限公司 | Veterinary baicalin injection liquid and preparing method thereof |
| CN104306881A (en) * | 2014-10-10 | 2015-01-28 | 天津瑞贝特科技发展有限公司 | Injection for preventing and treating poultry escherichia coli diseases and preparation method of injection |
| CN104397499A (en) * | 2014-12-24 | 2015-03-11 | 武汉华扬动物药业有限责任公司 | Feed additive for preventing or easing toxin infection of piglets at weaning period, and preparation method of feed additive |
| CN105250460A (en) * | 2015-10-21 | 2016-01-20 | 北京康华远景科技股份有限公司 | Drug composition for treating rabbit diarrhea and preparation methods of drug composition |
| CN113855768A (en) * | 2021-11-22 | 2021-12-31 | 河南宝威生物工程技术有限公司 | Traditional Chinese medicine composition for preventing piglet diarrhea and preparation method and application thereof |
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