CN108434369A - Treat the probiotics herbal mixture zymotic fluid of chicken colibacillosis - Google Patents
Treat the probiotics herbal mixture zymotic fluid of chicken colibacillosis Download PDFInfo
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- CN108434369A CN108434369A CN201810617052.1A CN201810617052A CN108434369A CN 108434369 A CN108434369 A CN 108434369A CN 201810617052 A CN201810617052 A CN 201810617052A CN 108434369 A CN108434369 A CN 108434369A
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- parts
- probiotics
- zymotic fluid
- herbal mixture
- chicken colibacillosis
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Abstract
The invention discloses a kind of probiotics herbal mixture zymotic fluids for treating chicken colibacillosis, it is prepared by following methods：1）Radix Astragali, the coptis, dark plum, the Chinese bulbul, Poria cocos, radix scutellariae, honeysuckle, folium isatidis, pueraria lobata, climbing groundsel, Divine Comedy, Radix Glycyrrhizae, hawthorn, rhizoma alismatis, myrobalan, alum, Cortex Phellodendri, malt are weighed, water is added, heats 30 ~ 50min, filtering, filtrate is collected, concentration, as water carries herb liquid；2）Lactobacillus bulgaricus is activated with lactobacillus plantarum, by 1:1 ~ 6 is seeded in MRS fluid nutrient mediums, and 20 ~ 30h of constant temperature incubation at 36 ~ 38 DEG C obtains compound probiotics preparation；3）By step 2）Compound probiotics preparation be inoculated into step 1）Water carry in herb liquid, inoculum concentration be Chinese medicinal liquor fraction 3 ~ 5%, fermentation, the probiotics herbal mixture zymotic fluid for the chicken colibacillosis that obtains medical treatment；The good antimicrobial effect of zymotic fluid of the present invention can safeguard intestinal microecology balance, improve immunity of organisms, and have the effect of there is inhibiting bacteria and diminishing inflammation.
Description
Technical field
The invention belongs to the Prevention Technique fields of chicken colibacillosis, and in particular to treat the probiotics of chicken colibacillosis
Herbal mixture zymotic fluid.
Background technology
Chicken colibacillosis is clinical common one of the chicken disease in China, is caused by enteropathogenic E. Coli.With hair
It is sick fast, the death rate is high, the feature of course of disease length, main disease type has that the death of embryo and young bird, septicemia, air bag is scorching, pericardium
Inflammation, salpingitis, enteritis, peritonitis and escherichia coli granuloma etc..Due to often and mycoplasma concurrent infection, and Chang Jifa
In animal infectious diease (such as newcastle disease, bird flu be puzzled, infective bronchitis, pasteurellosis), keep treatment very difficult.Mesh
Preceding disease has become the Important Infectious Diseases that industry is supported in harm, often results in huge economic loss.
Antibacterial drug therapy, but being excessively used due to such drug are mostly used for colibacillosis at present, caused
The Antibiotic Resistance of Escherichia coli constantly expands, and control effect declines, while residual of the antibacterials in body, easily flora is caused to lose
It adjusts, reduces meat egg product quality, affect human health and public health security.Chinese herbal medicine has natural, safe to use, nothing
Toxic side effect, without chemical residue, be not likely to produce the features such as drug resistance, but have the characteristics that curative effect is slow, it is presently commercially available
Although there are many Chinese herbal and crude drugs preparations type for treating chicken colibacillosis, practical effect is unsatisfactory；Chinese medicine develops
So far, processing method is too simple, and product is difficult to break away from thick, big, black feature, especially powder always, remains Chinese medicine
Effective and invalid target, and diameter of aspirin particle is big, and crude fiber content is high, and ill domestic animal especially poultry is difficult to digest and absorb, causes
Antibacterial or antiviral active ingredient are difficult to give full play to curative effect in Chinese medicine, while also resulting in the big of Chinese medicine precious resources
Amount waste.Therefore it develops a kind of Chinese medicine containing profitable probliotics and meets the defect that preparation can make up the above drug.
Invention content
It is an object of the present invention to provide a kind of materials to be easy, it is convenient to prepare, reduces medicament residue and toxic side effect, cheap
The probiotics herbal mixture zymotic fluid of the higher treatment chicken colibacillosis of curative effect.
The probiotics herbal mixture zymotic fluid of chicken colibacillosis is treated, it is prepared by following methods：
1）Weigh the Radix Astragali, coptis, dark plum, the Chinese bulbul, Poria cocos, radix scutellariae, honeysuckle, folium isatidis, pueraria lobata, climbing groundsel, Divine Comedy, sweet
Grass, hawthorn, rhizoma alismatis, myrobalan, alum, Cortex Phellodendri, malt add water, heat 30 ~ 50min, and filtrate, concentration, as water are collected in filtering
Carry herb liquid；
2）Lactobacillus bulgaricus is activated with lactobacillus plantarum, by 1:1 ~ 6 is seeded in MRS fluid nutrient mediums, constant temperature at 36 ~ 38 DEG C
20 ~ 30h is cultivated, compound probiotics preparation is obtained；
3）By step 2）Compound probiotics preparation be inoculated into step 1）Water carry in herb liquid, inoculum concentration be Chinese medicinal liquor integral
Several 3 ~ 5%, fermentation, the probiotics herbal mixture zymotic fluid for the chicken colibacillosis that obtains medical treatment；
Step 1）Described in 10 ~ 30 parts of Radix Astragali, 5 ~ 10 parts of the coptis, 6 ~ 15 parts of dark plum, 13 ~ 50 parts of the Chinese bulbul, Poria cocos 13 ~ 50
Part, 15 ~ 25 parts of radix scutellariae, 10 ~ 30 parts of honeysuckle, 5 ~ 15 parts of folium isatidis, 13 ~ 50 parts of pueraria lobata, 15 ~ 25 parts of climbing groundsel, Divine Comedy 10 ~ 20
Part, 6 ~ 30 parts of Radix Glycyrrhizae, 10 ~ 20 parts of hawthorn, 5 ~ 10 parts of rhizoma alismatis, 5 ~ 15 parts of myrobalan, 5 ~ 10 parts of alum, 5 ~ 20 parts of Cortex Phellodendri, malt 15 ~
50 parts；
20 parts of the Radix Astragali, 10 parts of the coptis, 10 parts of dark plum, 30 parts of the Chinese bulbul, 30 parts of Poria cocos, 20 parts of radix scutellariae, 20 parts of honeysuckle,
10 parts of folium isatidis, 30 parts of pueraria lobata, 20 parts of climbing groundsel, 15 parts of Divine Comedy, 20 parts of Radix Glycyrrhizae, 15 parts of hawthorn, 10 parts of rhizoma alismatis, 10 parts of myrobalan,
10 parts of alum, 10 parts of Cortex Phellodendri, 25 parts of malt；
Step 1）Described in be concentrated into 0.4 ~ 0.5g crude drugs in every milliliter of filtrate；
Step 2）Described in inoculative proportion be 1:4；
Step 3）Described in fermentation carried out in fermentation tank, 100 ~ 300rpm of rotating speed, 36 ~ 60h of time, 36 ~ 38 DEG C of temperature；
The rotating speed 200rpm, time 48h, 37 DEG C of temperature；
Step 3）Described in inoculum concentration be 4%.
Detailed description of the invention：
Pueraria lobata has relieving muscles diaphoresis, promotes the production of body fluid to quench thirst, Shengyang Zhixie, make in table solution and；The Chinese bulbul have it is clearing heat and detoxicating, cool blood is stopped
Dysentery has an astriction to intestinal mucosa, thus can antidiarrheal, be the key medicine for treating diarrhea due to damp-heat；There is the coptis heat-clearing and damp-drying drug, intestines Huang to make
It rushes down, weight, is advisable with the damp and hot card of stomach especially after hot dysentery；Radix scutellariae has heat-clearing and damp-drying drug, damp-heat dysentery；Cortex Phellodendri has diarrhea due to damp-heat,
Treat rush down dysentery；Honeysuckle have it is clearing heat and detoxicating, control heat toxin rush down dysentery, often associate with radix scutellariae treatment heat toxin diarrhea, have concurrently surname dissipate effect；
There is Divine Comedy promoting digestion and removing indigestion, invigorating and regulating stomach to treat splenasthenic diarrhea；Poria cocos have water retention due to hypofunction of the spleen, retention of water-damp, can invigorating the spleen but also
Dampness removing, energy complementary energy are rushed down；Dark plum has relieving diarrhea with astringents, is used for protracted dysentery and diarrhea；Myrobalan has relieving diarrhea with astringents, is used for protracted dysentery and diarrhea；Pool
It rushes down with energy clearing damp and promoting diuresis, it is more than to treat rush down dysentery；Radix Astragali has tonifying Qi, is suitable for spleen-lung Qi deficiency, diarrhea；Folium isatidis has heat-clearing
Cool blood, stomach energy poison；Climbing groundsel has heat-clearing, removing toxic substances, bacillary dysentery, diarrhea；Radix Glycyrrhizae has warm-natured, tonifying spleen and stomach, controls weakness of the spleen and the stomach, also has
There are coordinating the drug actions of a prescription, many formula compatibility this product；Hawthorn has food digesting stomach fortifying, treats dyspepsia, antidiarrheal；Malt has
It helps digestion in, treatment is weakness of the spleen and the stomach；Alum has astringing to arrest bleeding, can be used for endless diarrhea, and stimulation stomach lining causes reflectivity to be vomitted
It spits, prevents intestinal mucosa secretion, thus there is antidiarrheal effect.
It is provided by the invention it is a kind for the treatment of chicken colibacillosis Chinese medicine composition, with heat-clearing, cool blood, removing toxic substances, relieving diarrhea with astringents,
Protracted dysentery and diarrhea, coordinating the drug actions of a prescription, and adjust stomach damp and hot, treating both the exterior and interior；All medicines share is altogether at the agent of clearing away heat to stop diarrhea, drug in the present invention
Through organic assembling, dialectical compatibility and the effect of mutually assist, enhance party's heat-clearing and damp-drying drug antidiarrheal cures chicken large intestine to reach
The purpose of bacillosis；The Chinese medicine composition is fermented by lactobacillus bulgaricus, lactobacillus plantarum, the zymotic fluid of generation, energy
It safeguards intestinal microecology balance, improve immunity of organisms, and have the effect of there is inhibiting bacteria and diminishing inflammation；Selecting present invention treatment chicken
It when colibacillosis, catches 4 points of " wet, hot, antidiarrheal, protracted dysentery ", so-called " heat " is mainly shown as fever, draws white light stool, gas
Taste stench is unpleasant, and diseased chicken spirit is depressed, eye is narrowed, and likes on all fours, and the sleeping ground of individual chickens does not rise, and can not stand, tongue fur
Jaundice, rolling and rapid pulse, anorexia etc., if meeting the above symptom, it may be considered that being the diarrhea caused by " damp and hot "；It answers
Use main points：Have loose bowels it is white, and damp and hot, tongue fur whiten, rapid pulse.
The present invention provides a kind of probiotics herbal mixture zymotic fluids for treating chicken colibacillosis, it is by following methods
It prepares：1）Weigh Radix Astragali, the coptis, dark plum, the Chinese bulbul, Poria cocos, radix scutellariae, honeysuckle, folium isatidis, pueraria lobata, climbing groundsel, Divine Comedy,
Radix Glycyrrhizae, hawthorn, rhizoma alismatis, myrobalan, alum, Cortex Phellodendri, malt add water, heat 30 ~ 50min, and filtrate is collected in filtering, is concentrated, as
Water carries herb liquid；2）Lactobacillus bulgaricus is activated with lactobacillus plantarum, by 1:1 ~ 6 is seeded in MRS fluid nutrient mediums, and 36 ~ 38
20 ~ 30h of constant temperature incubation at DEG C obtains compound probiotics preparation；3）By step 2）Compound probiotics preparation be inoculated into step 1）
Water carry in herb liquid, inoculum concentration be Chinese medicinal liquor fraction 3 ~ 5%, fermentation, the probiotics for the chicken colibacillosis that obtains medical treatment
Herbal mixture zymotic fluid；The good antimicrobial effect of zymotic fluid of the present invention can safeguard intestinal microecology balance, improve immunity of organisms,
And has the effect of inhibiting bacteria and diminishing inflammation.
Description of the drawings
Fig. 1 lactobacillus bulgaricus, lactobacillus plantarum compound preparation growth curve.
Specific implementation mode
1 compound probiotics preparation ratio optimization technique of embodiment
Lactobacillus bulgaricus, lactobacillus plantarum are activated respectively：Lactobacillus bulgaricus, plant breast are taken out from -80 DEG C of refrigerators
Centrifuge tube is preheated with hand and dissolves its strain, two kinds of strains 100 of dissolving are then taken out in superclean bench by bacillus species
μ L are inoculated in respectively in two test tubes of the fluid nutrient medium containing 5mLMRS, 37 DEG C of culture 12h, and test tube then after incubation is each
100 μ L bacterium solutions are taken out, two test tubes of the fluid nutrient medium containing 5mLMRS are separately added into, 12h is cultivated at 37 DEG C, is then placed in 4 DEG C
Refrigerator is spare.
It is respectively 1 in lactobacillus bulgaricus and lactobacillus plantarum ratio:1、1:2、2:1 is seeded in MRS fluid nutrient mediums
（Lactic acid bacteria culture medium）On, constant temperature incubation 12h in 37 DEG C of incubators is measured with spectrophotometer in 600nm after culture
OD values, as a result show 1:OD values are maximum when 2；Lactobacillus bulgaricus and lactobacillus plantarum are pressed 1 respectively:2、1:3、1:4、1:
5、1:6 ratios, constant temperature incubation for 24 hours, measures OD values in 600nm with spectrophotometer after culture, compares in 37 DEG C of incubators
As a result size shows 1:OD values are maximum when 4, therefore by lactobacillus bulgaricus and lactobacillus plantarum 1：4 ratios are as compound benefit
The optimal proportion of probiotics preparation；Lactobacillus bulgaricus and lactobacillus plantarum 1：Growth curve is cultivated when 4 sees Fig. 1.
Embodiment 2 treats the preparation of the herb liquid of chicken colibacillosis
Weigh Radix Astragali 20g, coptis 10g, dark plum 10g, Chinese bulbul 30g, Poria cocos 30g, radix scutellariae 20g, honeysuckle 20g, folium isatidis
10g, pueraria lobata 30g, climbing groundsel 20g, Divine Comedy 15g, Radix Glycyrrhizae 20g, hawthorn 15g, rhizoma alismatis 10g, myrobalan 10g, alum 10g, Cortex Phellodendri
10g, malt 25g, total 315g are packed into 1000mL beakers, impregnate 30min, add 4 times of amounts i.e. 1260mL water（4 times refer to Chinese traditional medicine composition
The multiple of object weight）, pour into cooking pot for herbs, heat 40min, 2 layers of filtered through gauze are collected filtrate and poured into specified bottle；The dregs of a decoction
2 times of amount distilled water are added, 30min, 2 layers of filtered through gauze are heated in cooking pot for herbs, filtrate is poured into the bottle of filtrate just now；It is remaining
The dregs of a decoction add again 2 times amount distilled water, 30min, 2 layers of filtered through gauze are heated in cooking pot for herbs, filtrate is poured into bottle twice, in
Liquid amounts to 700mL, and it is 0.45g to be equivalent to crude drug amount in every milliliter of filtrate.
Embodiment 3 treats the preparation of the herb liquid of chicken colibacillosis
Weigh Radix Astragali 10g, coptis 10g, dark plum 15g, Chinese bulbul 20g, Poria cocos 50g, radix scutellariae 15g, honeysuckle 10g, folium isatidis
15g, pueraria lobata 20g, climbing groundsel 20g, Divine Comedy 20g, Radix Glycyrrhizae 10g, hawthorn 10g, rhizoma alismatis 10g, myrobalan 5g, alum 5g, Cortex Phellodendri 20g,
Malt 40g, total 315g are packed into 1000mL beakers, impregnate 30min, add 4 times of amounts i.e. 1260mL water（4 times refer to Chinese medicine composition weight
The multiple of amount）, pour into cooking pot for herbs, heat 40min, 2 layers of filtered through gauze are collected filtrate and poured into specified bottle；The dregs of a decoction add again
Enter 2 times of amount distilled water, 30min, 2 layers of filtered through gauze are heated in cooking pot for herbs, filtrate is poured into the bottle of filtrate just now；Remaining medicine
Slag adds 2 times of amount distilled water again, 30min, 2 layers of filtered through gauze is heated in cooking pot for herbs, filtrate is poured into bottle twice, herb liquid
Total 700mL, it is 0.45g to be equivalent to crude drug amount in every milliliter of filtrate.
Embodiment 4 treats the preparation of the herb liquid of chicken colibacillosis
Weigh Radix Astragali 30g, coptis 5g, dark plum 6g, Chinese bulbul 50g, Poria cocos 20g, radix scutellariae 25g, honeysuckle 30g, folium isatidis 5g, Pueraria lobota
Root 50g, climbing groundsel 20g, Divine Comedy 10g, Radix Glycyrrhizae 6g, hawthorn 20g, rhizoma alismatis 5g, myrobalan 8g, alum 5g, Cortex Phellodendri 5g, malt 15g, altogether
315g is packed into 1000mL beakers, impregnates 30min, adds 4 times of amounts i.e. 1260mL water（4 times refer to Chinese medicine composition weight multiple）,
It pours into cooking pot for herbs, heats 40min, 2 layers of filtered through gauze are collected filtrate and poured into specified bottle；The dregs of a decoction add 2 times of amount distillations
Water, heats 30min, 2 layers of filtered through gauze in cooking pot for herbs, and filtrate is poured into the bottle of filtrate just now；The remaining dregs of a decoction add 2 times of amounts again
Distilled water heats 30min, 2 layers of filtered through gauze in cooking pot for herbs, and filtrate is poured into bottle twice, and herb liquid amounts to 700mL,
It is 0.45g to be equivalent to crude drug amount in every milliliter of filtrate.
Embodiment 5 treats the preparation of the probiotics herbal mixture zymotic fluid of chicken colibacillosis
Compound probiotics preparation preparation method is prepared by embodiment 1, and water carries herb liquid and prepared by embodiment 2.
The 2.5L fermentation tanks that this experiment takes South Korea to produce carry out probiotics fermention, and parameter has：Temperature, rotating speed, compound benefit
Raw bacterium inoculum concentration, Chinese medicine dose, fermentation time, also pH, ventilatory capacity be not within setting range.It is fermented by fermentation tank,
Parameter setting is：Rotating speed 200rpm, time 48h, 37 DEG C of temperature, compound probiotics preparation inoculum concentration 20mL, water carry herb liquid
480mL ferments, the probiotics herbal mixture zymotic fluid for the chicken colibacillosis that obtains medical treatment.
Embodiment 6 treats the compliance test result of the probiotics herbal mixture zymotic fluid of chicken colibacillosis
Chicken colibacillosis, lesion is in intestines, characteristic of disease syndrome of dampness-heat, the new excrement of Clinical symptoms performance row's white, the key for the treatment of
Heat-clearing and damp-drying drug is wanted, the normal conduction function of small intestine is restored.
50 builds, the comparable healthy chicken of weight are selected, is randomly divided into 5 groups, one of which is as a control group；Remaining 4 groups of work
For experimental group, Chinese medicine is fed with by imagineering's chicken colibacillosis respectively after Escherichia coli disease symptoms occurs in experimental animal
（The herb liquid for the treatment of chicken colibacillosis prepared by embodiment 2）, Western medicine（Ciprofloxacin）, the probiotics for preparing of embodiment 3 it is multiple
Square Chinese medicine fermented liquid, distilled water, as Chinese medicine group, Western medicine group, zymotic fluid group, infected group；After treatment 7 days, 5 groups of realities are measured respectively
Test the caecum SIgA of animal（It the results are shown in Table 1）, duodenum, caecum histopathologic slide, Serum antioxidant indices（Malonaldehyde,
Glutathione peroxidase, superoxide dismutase）；Caecum SIgA the results are shown in Table 1, malonaldehyde, glutathione peroxidase
Enzyme, superoxide dismutase result are shown in Table 2,3,4 respectively；In table 1 ~ 4, the identical person's difference of same column shoulder mark lowercase is not notable（P
>0.05）, difference person's significant difference（P<0.05）, capitalization difference person's difference is extremely notable（P<0.01）；
The results show that the fungistatic effect of zymotic fluid is higher than the fungistatic effect of herbal mixture, but it is somewhat below Western medicine group therapeutic effect；
Factors, the probiotics herbal mixture zymotic fluids such as comprehensive poisonous side effect of medicine are more excellent.
The instructions of taking of the zymotic fluid：
1）Dose：Zymotic fluid and water are put together, and free water is taken；
2）Medication time：Every morning is primary, and noon is primary, primary at night.
3）Medicine temperature control system：It warmly takes；
4）Avoid item：During zymotic fluid is treated, reinforce the feeding management to chicken, ensures sufficient drinking-water, right place feeding
Particulate material.
It in one month imagineering and therapeutic process, treats 1 week, cures 8, improve 1, cure rate 84.3% has
Efficiency 100%；
Curative effect determinate standard：Whether Main Basiss chicken diarrhea symptom disappears, 7 days as one therapeutic course of taking medicine；
Specific curative effect is divided into three-level：Healing-chicken colibacillosis white symptom of having loose bowels completely disappears；Improvement-chicken colibacillosis is drawn
White dilute symptom partial disappearance；The colibacillosis of in vain-chicken white symptom of having loose bowels has no to disappear.
Claims (7)
1. treating the probiotics herbal mixture zymotic fluid of chicken colibacillosis, it is prepared by following methods：
1）Weigh the Radix Astragali, coptis, dark plum, the Chinese bulbul, Poria cocos, radix scutellariae, honeysuckle, folium isatidis, pueraria lobata, climbing groundsel, Divine Comedy, sweet
Grass, hawthorn, rhizoma alismatis, myrobalan, alum, Cortex Phellodendri, malt add water, heat 30 ~ 50min, and filtrate, concentration, as water are collected in filtering
Carry herb liquid；
2）The lactobacillus bulgaricus and lactobacillus plantarum of activation press 1:1 ~ 6 is seeded in MRS fluid nutrient mediums, permanent at 36 ~ 38 DEG C
Temperature 20 ~ 30h of culture, obtains compound probiotics preparation；
3）By step 2）Compound probiotics preparation be inoculated into step 1）Water carry in herb liquid, inoculum concentration be Chinese medicinal liquor integral
Several 3 ~ 5%, fermentation, the probiotics herbal mixture zymotic fluid for the chicken colibacillosis that obtains medical treatment；
Step 1）Described in 10 ~ 30 parts of Radix Astragali, 5 ~ 10 parts of the coptis, 6 ~ 15 parts of dark plum, 13 ~ 50 parts of the Chinese bulbul, Poria cocos 13 ~ 50
Part, 15 ~ 25 parts of radix scutellariae, 10 ~ 30 parts of honeysuckle, 5 ~ 15 parts of folium isatidis, 13 ~ 50 parts of pueraria lobata, 15 ~ 25 parts of climbing groundsel, Divine Comedy 10 ~ 20
Part, 6 ~ 30 parts of Radix Glycyrrhizae, 10 ~ 20 parts of hawthorn, 5 ~ 10 parts of rhizoma alismatis, 5 ~ 15 parts of myrobalan, 5 ~ 10 parts of alum, 5 ~ 20 parts of Cortex Phellodendri, malt 15 ~
50 parts.
2. the probiotics herbal mixture zymotic fluid for the treatment of chicken colibacillosis according to claim 2, it is characterised in that：Institute
20 parts of the Radix Astragali stated, 10 parts of the coptis, 10 parts of dark plum, 30 parts of the Chinese bulbul, 30 parts of Poria cocos, 20 parts of radix scutellariae, 20 parts of honeysuckle, folium isatidis
10 parts, 30 parts of pueraria lobata, 20 parts of climbing groundsel, 15 parts of Divine Comedy, 20 parts of Radix Glycyrrhizae, 15 parts of hawthorn, 10 parts of rhizoma alismatis, 10 parts of myrobalan, alum 10
Part, 10 parts of Cortex Phellodendri, 25 parts of malt.
3. the probiotics herbal mixture zymotic fluid of the treatment chicken colibacillosis according to claim 1 or 2, feature exist
In：Step 1）Described in be concentrated into 0.4 ~ 0.5g crude drugs in every milliliter of filtrate.
4. the probiotics herbal mixture zymotic fluid for the treatment of chicken colibacillosis according to claim 3, it is characterised in that：Step
Rapid 2）Described in inoculative proportion be 1:4.
5. the probiotics herbal mixture zymotic fluid for the treatment of chicken colibacillosis according to claim 4, it is characterised in that：Step
Rapid 3）Described in fermentation carried out in fermentation tank, 100 ~ 300rpm of rotating speed, 36 ~ 60h of time, 36 ~ 38 DEG C of temperature.
6. the probiotics herbal mixture zymotic fluid for the treatment of chicken colibacillosis according to claim 5, it is characterised in that：Institute
The rotating speed 200rpm that states, time 48h, 37 DEG C of temperature.
7. the probiotics herbal mixture zymotic fluid for the treatment of chicken colibacillosis according to claim 6, it is characterised in that：Step
Rapid 3）Described in inoculum concentration be 4%.
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