CN102793869A - Traditional Chinese medicine composition for controlling porcine viral diseases and preparation method thereof - Google Patents

Abstract

The invention discloses a traditional Chinese medicine composition for controlling the porcine viral diseases, comprising the following raw materials by weight: 150-250 parts of houttuynia, 60-90 parts of forsythia fruit, 60-120 parts of honeysuckle, 120-160 parts of rehmannia root, 80-100 parts of isatis root, 30-120 parts of rhubarb, 90-150 parts of balloon flower, 50-90 parts of mulberry leaves, 120-300 parts of plantain, 100-300 parts of cogongrass rhizome, and 80-120 parts of liquorice. The raw materials are processed by distillation concentration, decoction extraction, filtration sterilization, and packaging. The raw materials are cheap and easily-obtained, the cost is low, and the production process is simple. The medicine composition can expel heat, regulate the meridian, ventilate the lung, moisten the dryness syndrome and reduce the fever and has very good control efficiency on the porcine viral diseases (especially febrile disease).

Description

A kind of Chinese medicine composition that is used to prevent and treat the pig virus disease and preparation method thereof

Technical field

The present invention relates to a kind of Chinese medicine composition of preventing and treating the pig virus disease and preparation method thereof, belong to field of biological pharmacy for animals.

Background technology

The morbidity of current China live pig mainly show as multiple cause of disease mixed infection and secondary infection; Have again simultaneously that immunosuppressant, virus constantly make a variation, the enhanced problem of bacterial drug resistance; Cause present the cause of disease diversification clinically, disease is complicated, brings very big difficulty for the diagnosis and treatment of swine diseases.Strengthen the epidemic prevention except the sick kind of minorities such as swine fever, pig lung plague, pig erysipelas has vaccine, most bacterial disease viral diseases are also popularized vaccine at present and are prevented and treated.And have the vaccine of preventive effect maybe be when not inoculating, and just cause at nursery phase and die young, or cause the nursing sow postpartum syndrome, bring very big loss to aquaculture.Moreover; Because life-time service antibiotic and other medicated premix have increased very big pressure for the metabolism of body, pig immune system only receives certain interference; Make pathogenic microorganism produce very strong drug resistance simultaneously, and the increasing of drug dose use can cause medicine intravital residual pig.Along with attention and the Chinese medicine preparation technological development of the whole society to food safety, animal production safety, Chinese medicine is more and more obvious as the traditional and classical advantages of swine diseases means of prevention in farming and animal husbandry.

The modern medicine and pharmacology achievement in research shows that many Chinese medicines contain abundant polysaccharide, alkaloids, organic acid, glycoside, volatile oil and resinae etc.The Chinese medicine preparation of processing with these materials is used for animal, antiviral is arranged, antibacterium, the effect of anti-stress.Chinese medicine is adjusted with integral body, and Yin Yang balancing is characteristics, is comparatively ideal immunomodulator.Can improve the histiocytic activity of immune system, increase the growth of immune cell and synthesizing of antibody, regulate body's immunological function, improve the premunition of body, especially resist viral infection and morbific ability.Use the Chinese medicine safety non-toxic in addition, have no side effect, do not develop immunity to drugs and drug residue, can also absorb by facilitating digestion, improve pork quality and taste, in recent years, on veterinary clinic, be used widely, and effect is remarkable.

Summary of the invention

The object of the present invention is to provide a kind of curative effect high, have no side effect, be easy to popularize in an all-round way the Chinese medicine composition of the control pig virus disease of application.

Another object of the present invention also is to provide a kind of method for preparing of preventing and treating the Chinese medicine composition of pig virus disease.

In order to realize above purpose, the technical scheme that the present invention adopted is: the raw material by following parts by weight is formed: 150～250 parts of Herba Houttuyniae, 60～90 parts of Fructus Forsythiaes, 60～120 parts of Flos Loniceraes, 120～160 parts of Radix Rehmanniae, 80～100 parts of Radix Isatidis, 30～120 parts of Radix Et Rhizoma Rhei, 90～150 parts of Radix Platycodoniss, 50～90 parts on Folium Mori, 120～300 parts of Herba Plantaginiss, 100～300 parts of Rhizoma Imperataes, 80～120 parts in Radix Glycyrrhizae.

This process for preparing medicine comprises the steps:

(1) gets Herba Houttuyniae, Fructus Forsythiae, Flos Lonicerae, Radix Rehmanniae, Radix Isatidis, Radix Et Rhizoma Rhei, Radix Platycodonis, Folium Mori, Herba Plantaginis, Rhizoma Imperatae, Radix Glycyrrhizae; Cleaning chopping places in the alembic; Add the 4000mL distilled water, add thermal distillation and collect distillate 1000mL, it is subsequent use that distillate is concentrated into 500mL;

(2) medicinal residues in the step (1) are added 5～6 times of water gagings, intense fire boils 10～30min, and warm fire boils 50～80min, filters then, collects filtrating; For filtering residue, add 2～3 times of water gagings again, first intense fire boils 10～30min, and warm fire boils 50～80min, filters once more, and collection filtrating merges to twice filtrating together, continues heating with slow fire then, is syrupy shape to filtrating;

(3) adding 20% lime cream adjusting pH is 12～14, places 12h～24h, and using 50% sulfuric acid solution to regulate pH is 5～6, fully stirs placement 3～4h, filters;

(4) filtrating is transferred pH to 3～7 with 4%NaOH solution, places 3～4h, filtering and heating to extractum shape;

(5) above-mentioned steps (1) distillate and step (4) extractum liquid are merged, add the injection water, add sodium sulfite 0.5g, be adjusted to etc. and ooze with injecting NaCl solution, with packing behind the cellulose acetate filter membrane fine straining to 500mL;

(6) 121 ℃ of sterilization 20min, packing, room temperature preservation.

Drug action mechanism of the present invention is following:

The Herba Houttuyniae nature and flavor are hot, are slightly cold, and clearing away lung-heat cures mainly damp-heat dysentery, the lung abscess pus and blood; Fructus Forsythiae heat-clearing and toxic substances removing, detumescence dissipating blood stasis have certain curative effect to bird flu virus; Flos Lonicerae heat-clearing and toxic substances removing, wind-dispelling heat-dissipating have the spectrum antibacterial action; Radix Rehmanniae clearing away heat and cooling blood, YIN nourishing and the production of body fluid promoting cure mainly various hemorrhages, and low grade fever does not move back; The Radix Isatidis pharynx-clearing throat-benefiting, removing heat from blood cures mainly acute calenture, hyperpyrexia headache, jaundice due to damp-heat; Radix Et Rhizoma Rhei clearing away heat-fire, removing pathogenic heat from blood and toxic substance from the body, eliminating blood stasis and inducing menstruation have anti-infectious function, and various bacteria is had inhibitory action, and influenza virus is also had inhibitory action; Hemostasis arranged, protect the liver blood pressure lowering, the function of gallbladder promoting digestion promoting function, can be used as animal immunity potentiator, be used for the disease that immune function depression causes; Radix Platycodonis eliminating phlegm and stopping cough lung qi dispersing, moistening and cleaning throat sound producing, evacuation of pus; Grandmother's Folium Mori bitter in the mouth, sweet, cold in nature has effects such as dispelling wind and heat pathogens, clearing away lung-heat and moistening for dryness, antiinflammatory, resisting pathogenic microbes; Herba Plantaginis is sweet, cold, clearing away heat-damp and promoting diuresis, antiinflammatory detumescent are arranged, antitumaous effect relieves internal heat; The Rhizoma Imperatae sweet in the mouth, cold, cooling blood for hemostasis is arranged, clearing away heat and promoting diuresis, lung heat clearing gastric heat effect; Radix Glycyrrhizae has invigorating the spleen and replenishing QI, heat-clearing and toxic substances removing, and expelling phlegm for arresting cough, relieving spasm to stop pain, the effect of coordinating the actions of various ingredients in a prescription is described as " kings of hundred medicines ".All medicines and with having clearing away heat and expelling pathogen in the exterior, reducing heat and dispersing lung-QI is moisturized analgesicly, separates the pestilent toxicity effect.

Advantage of the present invention:

(1) raw material is a Chinese herbal medicine, and wide material sources do not contain any antibiotic and chemical hormone, do not have drug residue and side effect, and environmental protection is applicable to common swine diseases;

(2) production method is simple, and the experimental article and the reagent that need are more common;

(3) combine modern Chinese medicine to gain knowledge according to Traditional Chinese medical theory, reasonable compatibility strengthens cooperation, sets up prescription and confirms optimal dose;

(4) clearing away heat and expelling pathogen in the exterior, reducing heat and dispersing lung-QI is moisturized analgesicly, has the extremely strong various pathogenic bacteria viral functions of killing, and can also make every effort to promote the into recovery of physiological function by human body immunity improving.

The specific embodiment

Through embodiment the present invention is described further below.

Embodiment 1

Raw material is formed: Herba Houttuyniae 160g; Fructus Forsythiae 60g, Flos Lonicerae 120g, Radix Rehmanniae 150g, Radix Isatidis 90g, Radix Et Rhizoma Rhei 90g, Radix Platycodonis 120g, Folium Mori 80g, Herba Plantaginis 180g, Rhizoma Imperatae 150g, Radix Glycyrrhizae 120g.

Method for preparing is as follows:

(1) gets Herba Houttuyniae, Fructus Forsythiae, Flos Lonicerae, Radix Rehmanniae, Radix Isatidis, Radix Et Rhizoma Rhei, Radix Platycodonis, old Folium Mori, Herba Plantaginis, Rhizoma Imperatae, Radix Glycyrrhizae; Cleaning chopping places in the alembic; Add the 4000mL distilled water, add thermal distillation and collect distillate 1000mL, it is subsequent use that distillate is concentrated into 500mL;

(2) with adding 5 times of water gagings in the medicinal residues of step (1), intense fire boils 10min, and warm fire boils 50min, filters then, collects filtrating; For filtering residue, add 2 times of water gagings again, first intense fire boils 10min, and warm fire boils 50min, filters once more, and collection filtrating merges to twice filtrating together, continues heating with slow fire then, is syrupy shape to filtrating;

(3) adding 20% lime cream adjusting pH is 12, places 12h, and using 50% sulfuric acid solution to regulate pH is 5.3, fully stirs placement 3h, filters;

(4) filtrating is transferred pH to 5 with 4%NaOH solution, places 3h, filtering and heating to extractum shape;

(5) above-mentioned steps (1) distillate and step (4) extractum liquid are merged, add the injection water, add sodium sulfite 0.5g, be adjusted to etc. and ooze with injecting NaCl, with packing behind the cellulose acetate filter membrane fine straining to 500mL;

(6) 121 ℃ of sterilization 20min, packing, room temperature preservation.

Embodiment 2

Raw material is formed: Herba Houttuyniae 150g; Fructus Forsythiae 80, Flos Lonicerae 100g, Radix Rehmanniae 120g, Radix Isatidis 80g, Radix Et Rhizoma Rhei 120g, Radix Platycodonis 90g, Folium Mori 90g, Herba Plantaginis 120g, Rhizoma Imperatae 100g, Radix Glycyrrhizae 80g.

Method for preparing:

(1) gets Herba Houttuyniae, Fructus Forsythiae, Flos Lonicerae, Radix Rehmanniae, Radix Isatidis, Radix Et Rhizoma Rhei, Radix Platycodonis, old Folium Mori, Herba Plantaginis, Rhizoma Imperatae, Radix Glycyrrhizae; Cleaning chopping places in the alembic; Add the 4000mL distilled water, add thermal distillation and collect distillate 1000mL, it is subsequent use that distillate is concentrated into 500mL;

(2) with adding 6 times of water gagings in the medicinal residues of step (1), intense fire boils 30min, and warm fire boils 60min, filters then, collects filtrating; For filtering residue, add 3 times of water gagings again, first intense fire boils 30min, and warm fire boils 80min, filters once more, and collection filtrating merges to twice filtrating together, continues heating with slow fire then, is syrupy shape to filtrating;

(3) adding 20% lime cream adjusting pH is 13, places 18h, and using 50% sulfuric acid solution to regulate pH is 6, fully stirs placement 4h, filters;

(4) filtrating is transferred pH to 3 with 4%NaOH solution, places 4h, filtering and heating to extractum shape;

(5) above-mentioned steps (1) distillate and step (4) extractum liquid are merged, add the injection water, add sodium sulfite 0.5g, be adjusted to etc. and ooze with injecting NaCl, with packing behind the cellulose acetate filter membrane fine straining to 500mL;

(6) 121 ℃ of sterilization 20min, packing, room temperature preservation.

Embodiment 3

Raw material is formed: Herba Houttuyniae 250g; Fructus Forsythiae 90g, Flos Lonicerae 60g, Radix Rehmanniae 160g, Radix Isatidis 100g, Radix Et Rhizoma Rhei 30g, Radix Platycodonis 150g, Folium Mori 50g, Herba Plantaginis 300g, Rhizoma Imperatae 300g, Radix Glycyrrhizae 100g.

Method for preparing is as follows:

(1) gets Herba Houttuyniae, Fructus Forsythiae, Flos Lonicerae, Radix Rehmanniae, Radix Isatidis, Radix Et Rhizoma Rhei, Radix Platycodonis, old Folium Mori, Herba Plantaginis, Rhizoma Imperatae, Radix Glycyrrhizae; Cleaning chopping places in the alembic; Add the 4000ml distilled water, add thermal distillation and collect distillate 1000ml, it is subsequent use that distillate is concentrated into 500ml;

(2) with adding 5.5 times of water gagings in the medicinal residues of step (1), intense fire boils 20min, and warm fire boils 80min, filters then, collects filtrating; For filtering residue, add 2.5 times of water gagings again, first intense fire boils 20min, and warm fire boils 60min, filters once more, and collection filtrating merges to twice filtrating together, continues heating with slow fire then, is syrupy shape to filtrating;

(3) adding 20% lime cream adjusting pH is 14, places 24h, and using 50% sulfuric acid solution to regulate pH is 5, fully stirs placement 3.5h, filters;

(4) filtrating is transferred pH to 7 with 4%NaOH solution, places 3.5h, filtering and heating to extractum shape;

(5) above-mentioned steps (1) distillate and step (4) extractum liquid are merged, add the injection water, add sodium sulfite 0.5g, be adjusted to etc. and ooze with injecting NaCl, with packing behind the cellulose acetate filter membrane fine straining to 500mL;

(6) 121 ℃ of sterilization 20min, packing, room temperature preservation.

Clinical experiment

Material and method:

(1) test drug: the medicine injection liquor of the embodiment of the invention 1 preparation.

(2) experimental animal: 40 average weights are the healthy piglet that 35-40kg does not carry pathogenic bacteria, are provided by plant of Zhengzhou Houyi Pharmaceutical Co., Ltd..

(3) major in strain: the swine fever virus strain is provided by the supervision of Henan Province's veterinary drug.

(4) test method

100 average weights are the healthy piglet of 35～40kg, are equally divided into 4 groups, and 10 every group, first group is the blank group that does not infect; Second group is the infection swine fever matched group of not administration; The 3rd group of prevention group; After infecting swine fever, use this medication therapy groups for the 4th group; The 5th group is with penicillin injection liquid treatment group after infecting swine fever.The injection consumption is every 1kg body weight intramuscular injection 0.5mL, once a day, and logotype three days.Equal conditions is fed down, observes the situation such as clinical change, healing, improvement, death of disease pig every day, the line item of going forward side by side.

Experimental result and analysis

Table 1 this patent injection is observed the swine fever therapeutic effect

Occur behind the four groups of infected pigs's Pestivirus in back that fever of the body is not eaten, the body surface fever, fervescence to 41～42%, eyes are crimson, appetite is useless exhausted; Thirsty greedy drink is then suffered from diarrhoea after the first constipation, and the row step waves; The blue symptom such as rubescent of skin can find out that from second group Chinese medicine of the present invention has the excellent prevention effect in ear, abdomen and the lower limb, only has a pig ill in 20 examples; And final spontaneous recovery; Can find out that with the 3rd group of contrast traditional antibiotic and Chinese medicine of the present invention all can resist courses of infection well respectively for fourth, fifth group, but obviously be superior to the antibiotic therapy group with the Chinese medicine preparation therapeutic effect, cure rate is respectively 86.7% and 35.7%.

Claims (3)

1. Chinese medicine composition that is used to prevent and treat the pig virus disease, it is characterized in that: the raw material by following parts by weight is formed: 150～250 parts of Herba Houttuyniae, 60～90 parts of Fructus Forsythiaes, 60～120 parts of Flos Loniceraes, 120～160 parts of Radix Rehmanniae, 80～100 parts of Radix Isatidis, 30～120 parts of Radix Et Rhizoma Rhei, 90～150 parts of Radix Platycodoniss, 50～90 parts on Folium Mori, 120～300 parts of Herba Plantaginiss, 100～300 parts of Rhizoma Imperataes, 80～120 parts in Radix Glycyrrhizae.

2. a kind of Chinese medicine composition that is used to prevent and treat the pig virus disease as claimed in claim 1, it is characterized in that: the said components preferred dose is: 160 parts of Herba Houttuyniae, 60 parts of Fructus Forsythiaes; 120 parts of Flos Loniceraes, 150 parts of Radix Rehmanniae, 90 parts of Radix Isatidis; 90 parts of Radix Et Rhizoma Rhei, 120 parts of Radix Platycodoniss, 80 parts on Folium Mori; 180 parts of Herba Plantaginiss, 150 parts of Rhizoma Imperataes, 120 parts in Radix Glycyrrhizae.

3. method for preparing that is used to prevent and treat the Chinese medicine preparation of pig virus disease according to claim 1 is characterized in that: may further comprise the steps:

(1) gets Herba Houttuyniae, Fructus Forsythiae, Flos Lonicerae, Radix Rehmanniae, Radix Isatidis, Radix Et Rhizoma Rhei, Radix Platycodonis, old Folium Mori, Herba Plantaginis, Rhizoma Imperatae, Radix Glycyrrhizae; Cleaning chopping places in the alembic; Add the 4000mL distilled water, add thermal distillation and collect distillate 1000mL, it is subsequent use that distillate is concentrated into 500mL;

(2) medicinal residues in the step (1) are added 5～6 times of water gagings, intense fire boils 10～30min (providing value range with the yellow part of subscript), and warm fire boils 50～80min, filters then, collects filtrating; For filtering residue, add 2～3 times of water gagings again, first intense fire boils 10～30min, and warm fire boils 50～80min, filters once more, and collection filtrating merges to twice filtrating together, continues heating with slow fire then, is syrupy shape to filtrating;

(3) adding 20% lime cream adjusting pH is 12～14, places 12h～24h, and using 50% sulfuric acid solution to regulate pH is 5～6, fully stirs placement 3～4h, filters;

(4) filtrating is transferred pH to 3～7 with 4%NaOH solution, places 3～4h, filtering and heating to extractum shape;

(5) above-mentioned steps (1) distillate and step (4) extractum liquid are merged, add the injection water, add sodium sulfite 0.5g, be adjusted to etc. and ooze with injecting NaCl solution, with packing behind the cellulose acetate filter membrane fine straining to 500mL;

(6) 121 ℃ of sterilization 20min, packing, room temperature preservation.