CN101947270A - Aerosol for preventing and controlling pullorum disease and preparation method thereof - Google Patents

Aerosol for preventing and controlling pullorum disease and preparation method thereof Download PDF

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Publication number
CN101947270A
CN101947270A CN2010105070695A CN201010507069A CN101947270A CN 101947270 A CN101947270 A CN 101947270A CN 2010105070695 A CN2010105070695 A CN 2010105070695A CN 201010507069 A CN201010507069 A CN 201010507069A CN 101947270 A CN101947270 A CN 101947270A
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grams
aerosol
radix
water
value
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CN101947270B (en
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吴红云
李建正
李凤娟
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Henan Hou Yi Industry Group Co., Ltd.
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Zhengzhou Houyi Pharmaceutical Co Ltd
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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Abstract

The invention discloses an aerosol for preventing and controlling pullorum disease and a preparation method thereof. The aerosol is prepared from the following raw materials by weight/volume: 50 to 200 grams of golden thread, 50 to 200 grams of amur corktree bark, 50 to 100 grams of rhubarb, 50 to 200 grams of baical skullcap root, 50 to 200 grams of indigowoad root, 50 to 100 grams of liquoric root, 10 to 80 grams of propellant, 50 to 150 milliliters of propylene glycol, 100 to 350 milliliters of ethanol and the balance of water. The preparation method comprises the following steps of: extracting active ingredients of the golden thread, the amur corktree bark, the rhubarb, the indigowoad root and the liquoric root and extracting active ingredients of the baical skullcap root; and mixing the extracts, adjusting pH value, filtering, adding active carbon to adsorb impurities, leaching, adding the propylene glycol, the ethanol and the water, adjusting the pH value to an acid state, adding the water until the volume is 1,000 milliliters, mixing uniformly, packaging and filling after a product is up to the standard and introducing the propellant after covering so as to obtain the aerosol. The product has the effects of clearing away heat, reducing fire, diminishing inflammation and relieving dysentery and has the advantages of convenient administration, safety, nontoxicity, no medicament residue, difficult medicament tolerance and high efficiency.

Description

A kind of aerosol of preventing and treating the white scour of chicken and preparation method thereof that is used to
Technical field
The present invention is specifically related to a kind of aerosol that is used to prevent and treat the white scour of chicken, also relates to the preparation method of this aerosol simultaneously.
Background technology
The white scour of chicken is a kind of acute, the deteriorated blood sexually transmitted disease that is caused by Salmonella, and generation is all arranged all over the world, and M ﹠ M is all high, and the white scour of chicken is the highest with interior chickling M ﹠ M with 2 ages age ~ 3 week in week.Sick chicken is drawn white loose stool and sticks with paste anus with chickling is principal character, on the organs such as slow-witted upright, drowsiness, dysentery of performance loss of appetite, drop-head and cardiac muscle, liver, lung necrobiotic nodule is arranged.Pathology cuts open inspection: visible absorption of yolk at its is bad, yolk sac shrinkage, faint yellow, grease-like and cheesy that content is; The visible suppurative arthritis of part chicken, liver enlargement, hemorrhage is khaki, and the big canescence necrosis region of foxtail millet grain is arranged, cholecystectasia, spleen enlargement, hemorrhage, quality becomes fragile; Pathological change such as the part chicken occurs that lungs hyperemia, kidney enlargement, caecum are expanded, cardiac dilatation, pneumonia and intestine and small intestine are hemorrhage.The white scour of chicken has had a strong impact on brood survival rate and chicken group quality at present, causes enormous economic loss to aquaculture.
At present, mainly adopt antibacterials to prevent and treat the white scour of chicken, but antibiotic improper use or abuse often do not reach this sick purpose of control, also usually cause the appearance of drug residue and anti-bacterial strain, therapeutic effect is poor.
Summary of the invention
The object of the invention is to provide a kind of aerosol that is used to prevent and treat the white scour of chicken, easily develops immunity to drugs when solve adopting the antibiotic therapy white scour of chicken, medicine is residual in the chicken body, the problem of therapeutic effect difference.
In order to realize the object of the invention, technical program of the present invention lies in adopting a kind of aerosol that is used to prevent and treat the white scour of chicken, this aerosol is made by following raw material: Rhizoma Coptidis 50 ~ 200 grams, Cortex Phellodendri 50 ~ 200 grams, Radix Et Rhizoma Rhei 50 ~ 100 grams, Radix Scutellariae 50 ~ 200 grams, Radix Isatidis 50 ~ 200 grams, Radix Glycyrrhizae 50 ~ 100 grams, propellant 10 ~ 80g, propylene glycol 50 ~ 150ml, ethanol 100 ~ 350ml, water: surplus.
The consumption of described 7 flavor Chinese medicines is preferably: Rhizoma Coptidis 180 grams, Cortex Phellodendri 160 grams, Radix Et Rhizoma Rhei 70 grams, Radix Scutellariae 100 grams, Radix Isatidis 130 grams and Radix Glycyrrhizae 60 grams.
Described propellant is a kind of or its combination in any in dichlorodifluoromethane, dichlorotetra-fluoroethane, liquefied butane gas, the isceon.
Technical program of the present invention also lies in adopting a kind of preparation method that is used to prevent and treat the aerosol of the white scour of chicken, may further comprise the steps:
1) takes by weighing Rhizoma Coptidis, Cortex Phellodendri, Radix Et Rhizoma Rhei, Radix Isatidis, the Radix Glycyrrhizae 5 flavor medicines of described weight, add decocting
Boil three times, merge three times decocting liquid, filter, filtrate concentrates, and gets 5 flavor Chinese medicine extract A;
2) take by weighing the Radix Scutellariae of described weight, decoct with water three times, merge three times decocting liquid, filter, filtrate concentrates the back and regulates pH value to acid, leaves standstill after insulation, washes the adjusting pH value through washing, ethanol again, Radix Scutellariae extract B;
3) merging 5 flavor Chinese medicine extract and Radix Scutellariae extracts are concentrated into clear paste, regulate pH value, leave standstill the back and filter, and add active carbon in filtrate, leave standstill after stirring, and sucking filtration gets liquor C afterwards;
4) in liquor C, add propylene glycol, ethanol and water, regulate pH value, filter, get filtrate D to acid;
5) in filtrate D, add water and be settled to 1000ml, mix homogeneously, after the assay was approved, and packing, propellant is poured in embedding behind the gland, promptly obtain aerosol.
Concrete extraction process parameter is in the step 1): three decoctions add the water of 8 ~ 10 times of 5 flavor Chinese medicine gross weights respectively, wherein for the first time decoct 2h, and second, third time decocts 1.5h respectively.
Step 2) concrete extraction process parameter is in: three decoctions add the water of 8 ~ 10 times of Radix Scutellariae weight respectively, wherein for the first time decoct 2h, and second, third time decocts 1.5h respectively; 5 ~ 10 times of filtrate simmer down to Radix Scutellariae weight, acid ph value 1 ~ 2,60 ℃ ~ 80 ℃ of holding temperatures, temperature retention time are that 1 ~ 3h and time of repose are 16 ~ 24h; Ethanol is washed back pH value 6.5 ~ 7.5.
Concrete extraction process parameter is in the step 3): concentrate at 60 ℃ ~ 80 ℃, the relative density of clear paste is 1.1 ~ 1.3, and pH value is 6.5 ~ 7.5, and the addition of active carbon is a filtrate 1% ~ 2%.
PH value described in the step 4) is 5.0 ~ 6.0.
Beneficial effect of the present invention: product of the present invention has the effect of clearing away heat-fire, anti-inflammtory anti-dysentery, and medication is convenient, safe, nontoxic, no drug residue, is difficult for producing drug resistance, efficient.
The specific embodiment
Come further to set forth the preparation method of medicine of the present invention by the following examples.
Embodiment 1
A, a kind of consumption that is used to prevent and treat each raw material of aerosol of the white scour of chicken are:
This aerosol is made by following raw material: Rhizoma Coptidis 180 grams, Cortex Phellodendri 160 grams, Radix Et Rhizoma Rhei 70 grams, Radix Isatidis 130 grams, Radix Glycyrrhizae 60 grams, Radix Scutellariae 100 grams, propellant 10 grams, wherein propellant is dichlorodifluoromethane, propylene glycol 100ml, ethanol 180ml, water: surplus.
B, preparation method are as follows:
1) takes by weighing Rhizoma Coptidis 180 grams, Cortex Phellodendri 160 grams, Radix Et Rhizoma Rhei 70 grams, Radix Isatidis 130 grams, Radix Glycyrrhizae 60 grams respectively, add the water of 9 times of 5 flavor Chinese medicine gross weights, decoct three times, 2h for the first time, twice each 1.5h in back merges three times decocting liquid, filter, concentrated filtrate, the extract A of five kinds of Chinese medicines;
2) take by weighing Radix Scutellariae 100 grams, add the water of 9 times of Radix Scutellariae weight, decoct three times, 2h for the first time, twice each 1.5h in back merges three times decocting liquid, filter, be concentrated into 8 times of Radix Scutellariae weight, the pH value of regulating concentrated solution is to pH1 ~ 2, through 60 ℃ of insulation 3h, leave standstill 20h again, must precipitate afterwards, wash with water to Ph5.0, the ethanol of reuse 70% is washed till pH6.5, gets the baicalin extract B;
3) united extraction thing A and extract B are concentrated into 70 ℃ of relative densities and are 1.2 clear paste, and adjust pH to 7.0 leaves standstill, and filter, and add 1% active carbon in filtrate, leave standstill sucking filtration behind the 30min after stirring, liquor C;
4) in liquor C, add 100 ml propylene glycol, 180ml ethanol and water, transfer pH to 5.5, filter, get filtrate D;
5) add water to filtrate D and be settled to 1000ml, mix homogeneously after the assay was approved, is sub-packed in 10 bottles, pours the dichlorodifluoromethane of 10 grams behind the embedding gland, promptly gets aerosol.
Embodiment 2
A, a kind of consumption that is used to prevent and treat each raw material of aerosol of the white scour of chicken are:
This aerosol is made by following raw material: Rhizoma Coptidis 60 grams, Cortex Phellodendri 100 grams, Radix Et Rhizoma Rhei 55 grams, Radix Isatidis 180 grams, Radix Glycyrrhizae 50 grams, Radix Scutellariae 170 grams, propellant 18 grams, wherein propellant is liquefied butane gas, propylene glycol 50ml, ethanol 100ml, water: surplus.
B, preparation method are as follows:
1) takes by weighing Rhizoma Coptidis 60 grams, Cortex Phellodendri 100 grams, Radix Et Rhizoma Rhei 55 grams, Radix Isatidis 180 grams, Radix Glycyrrhizae 50 grams respectively, add the water of 8 times of 5 flavor Chinese medicine gross weights, decoct three times, for the first time decoct 2h, the back respectively decocts 1.5h twice, merges three times decocting liquid, filter, concentrated filtrate, the extract A of five kinds of Chinese medicines;
2) take by weighing Radix Scutellariae 170 grams, add the water of 8 times of Radix Scutellariae weight, decoct three times, 2h for the first time, twice each 1.5h in back merges three times decocting liquid, filter, be concentrated into 6 times of Radix Scutellariae weight, the pH value of regulating concentrated solution is to pH1 ~ 2, through 80 ℃ of insulations 1 hour, leave standstill 24h again, must precipitate afterwards, wash with water to Ph5.0, the ethanol of reuse 70% is washed till pH7.0, gets the baicalin extract B;
3) united extraction thing A and extract B are concentrated into 80 ℃ of relative densities and are 1.1 clear paste, and adjust pH to 6.5 leaves standstill, and filter, and add 1.5% active carbon in filtrate, leave standstill sucking filtration behind the 30min after stirring, liquor C;
4) in liquor C, add 50 ml propylene glycol, 100ml ethanol and water, transfer pH to 5.0, filter, get filtrate D;
5) add water to filtrate D and be settled to 1000ml, mix homogeneously after the assay was approved, is sub-packed in 10 bottles, pours 18 gram liquefied butane gas behind the embedding gland, promptly gets aerosol.
Embodiment 3
A, a kind of consumption that is used to prevent and treat each raw material of aerosol of the white scour of chicken are:
This aerosol is made by following raw material: Rhizoma Coptidis 90 gram, Cortex Phellodendri 55 grams, Radix Et Rhizoma Rhei 100 grams, Radix Isatidis 60 grams, Radix Glycyrrhizae 90 grams, Radix Scutellariae 60 grams, propellant 30 grams, wherein propellant is isceon and dichlorotetra-fluoroethane totally 30 grams, propylene glycol 150ml, ethanol 300ml, water: surplus.
B, preparation method are as follows:
1) takes by weighing Rhizoma Coptidis 90 grams, Cortex Phellodendri 55 grams, Radix Et Rhizoma Rhei 100 grams, Radix Isatidis 60 grams, Radix Glycyrrhizae 90 grams respectively, add the water of 10 times of 5 flavor Chinese medicine gross weights, decoct three times, 2h for the first time, twice each 1.5h in back merges three times decocting liquid, filter, concentrated filtrate, the extract A of five kinds of Chinese medicines;
2) take by weighing Radix Scutellariae 60 grams, add the water of 10 times of Radix Scutellariae weight, decoct three times, 2h for the first time, twice each 1.5h in back merges three times decocting liquid, filter, be concentrated into 9 times of Radix Scutellariae weight, the pH value of regulating concentrated solution is to pH1 ~ 2, through 65 ℃ of insulation 2h, leave standstill 16h again, must precipitate afterwards, wash with water to Ph5.0, the ethanol of reuse 70% is washed till pH7.5, gets the baicalin extract B;
3) united extraction thing A and extract B are concentrated into 60 ℃ of relative densities and are 1.3 clear paste, and adjust pH to 7.5 leaves standstill, and filter, and add 2% active carbon in filtrate, leave standstill sucking filtration behind the 30min after stirring, liquor C;
4) in liquor C, add 150 ml propylene glycol, 300ml ethanol and water, transfer pH to 6.0, filter, get filtrate D;
5) add water to filtrate D and be settled to 1000ml, mix homogeneously after the assay was approved, is sub-packed in 10 bottles, pours the isceon and the dichlorotetra-fluoroethane of 30 grams behind the embedding gland, promptly gets aerosol.
Below further set forth the clinical efficacy of medicine of the present invention by testing example.
Test example 1
1, test material
Anthology invention aerosol, control drug: gentamycin; Experimental animal: the chickling of 14 ~ 21 ages in days, chickling draw white loose stool and generation to stick with paste the anus phenomenon, loss of appetite, slow-witted upright, drowsiness, the dysentery of drop-head.It is bright to cut open probatio inspectionem pecuoarem, and it is the Pullorum Disease disease.
2, EXPERIMENTAL DESIGN
Select 500 chickling at random, be equally divided into 5 groups, test the product of one group of spray delivery example 1 of the present invention, test the product of two groups of spray delivery examples 2 of the present invention, test the product of three groups of spray delivery examples 3 of the present invention, test four groups of drinking-water administration gentamycin reference substances, test five groups and drink normal saline.
3, test method
First three groups is sprayed administration, successive administration 6 days according to the 100ml/ group to each group chicken; The 4th group of each consumption 1000~1500 units, the administration 6 days of drinking water continuously; The 5th group, freely drink water; Situations such as the clinical change of observation every day trouble chicken, healing, improvement, death, the line item of going forward side by side.
4, curative effect judging standard
Cure rate: after the off-test, sick chicken transference cure, spirit takes a turn for the better, and appetite increases, and recovers the ratio that normal chicken accounts for this group chicken fully.
Effective percentage: after the off-test, the chicken that fowl disease symptom, spirit, appetite are clearly better accounts for the ratio of this group chicken.
Inefficiency: symptom appears in duration of test, and the chicken that still loses transference cure after the off-test accounts for the ratio of this group chicken.
5, result of the test sees Table 1
Figure 2010105070695100002DEST_PATH_IMAGE001
Result of the test shows that the embodiment of the invention 1 prepared aerosol has significant curative effect to the white scour of chicken, compares cure rate with gentamycin and improves 5%, illustrates that the present invention has the obvious treatment effect to the white scour of chicken.

Claims (8)

1. aerosol that is used to prevent and treat the white scour of chicken, it is characterized in that: this aerosol is made by following raw material: Rhizoma Coptidis 50 ~ 200 grams, Cortex Phellodendri 50 ~ 200 grams, Radix Et Rhizoma Rhei 50 ~ 100 grams, Radix Scutellariae 50 ~ 200 grams, Radix Isatidis 50 ~ 200 grams, Radix Glycyrrhizae 50 ~ 100 grams, propellant 10 ~ 80g, propylene glycol 50 ~ 150ml, ethanol 100 ~ 350ml, surplus are water.
2. the aerosol that is used to prevent and treat the white scour of chicken according to claim 1 is characterized in that: the consumption of described 7 flavor Chinese medicines is respectively: Rhizoma Coptidis 180 grams, Cortex Phellodendri 160 grams, Radix Et Rhizoma Rhei 70 grams, Radix Scutellariae 100 grams, Radix Isatidis 130 grams and Radix Glycyrrhizae 60 grams.
3. the aerosol that is used to prevent and treat the white scour of chicken according to claim 1 is characterized in that: described propellant is a kind of or its combination in any in dichlorodifluoromethane, dichlorotetra-fluoroethane, liquefied butane gas, the isceon.
4. one kind as each described preparation method that is used to prevent and treat the aerosol of the white scour of chicken among the claim 1-3, and it is characterized in that: described preparation method may further comprise the steps:
1) take by weighing Rhizoma Coptidis, Cortex Phellodendri, Radix Et Rhizoma Rhei, Radix Isatidis, the Radix Glycyrrhizae 5 flavor medicines of described weight, decoct with water three times, merges three times decocting liquid, filter, filtrate concentrates, the 5 Chinese medicine extract A that distinguish the flavor of;
2) take by weighing the Radix Scutellariae of described weight, decoct with water three times, merge three times decocting liquid, filter, filtrate concentrates the back and regulates pH value to acid, leaves standstill after insulation, washes the adjusting pH value through washing, ethanol again, Radix Scutellariae extract B;
3) united extraction thing A and extract B are concentrated into clear paste, regulate pH value, leave standstill the back and filter, and add active carbon in filtrate, leave standstill after stirring, and sucking filtration gets liquor C afterwards;
4) in liquor C, add propylene glycol, ethanol and water, regulate pH value, filter, get filtrate D to acid;
5) in filtrate D, add water and be settled to 1000ml, mix homogeneously, after the assay was approved, and packing, propellant is poured in embedding behind the gland, promptly obtain aerosol.
5. the preparation method that is used to prevent and treat the aerosol of the white scour of chicken according to claim 4, it is characterized in that: concrete extraction process parameter is in the step 1): three decoctions add the water of 8 ~ 10 times of 5 flavor Chinese medicine gross weights respectively, wherein for the first time decoct 2h, second, third time decocts 1.5h respectively.
6. the preparation method that is used to prevent and treat the aerosol of the white scour of chicken according to claim 4, it is characterized in that: step 2) in concrete extraction process parameter be: decoct the water that adds 8 ~ 10 times of Radix Scutellariae weight respectively three times, wherein for the first time decoct 2h, second, third time decocts 1.5h respectively; 5 ~ 10 times of filtrate simmer down to Radix Scutellariae weight, acid ph value 1 ~ 2,60 ℃ ~ 80 ℃ of holding temperatures, temperature retention time are that 1 ~ 3h and time of repose are 16 ~ 24h; Ethanol is washed back pH value 6.5 ~ 7.5.
7. the preparation method that is used to prevent and treat the aerosol of the white scour of chicken according to claim 4, it is characterized in that: concrete extraction process parameter is in the step 3): concentrate at 60 ℃ ~ 80 ℃, the relative density of clear paste is 1.1 ~ 1.3, and pH value is 6.5 ~ 7.5, and the addition of active carbon is a filtrate 1% ~ 2%.
8. the preparation method that is used to prevent and treat the aerosol of the white scour of chicken according to claim 4, it is characterized in that: pH value described in the step 4) is 5.0 ~ 6.0.
CN2010105070695A 2010-10-14 2010-10-14 Aerosol for preventing and controlling pullorum disease and preparation method thereof Expired - Fee Related CN101947270B (en)

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Cited By (3)

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CN102793869A (en) * 2012-08-23 2012-11-28 郑州后羿制药有限公司 Traditional Chinese medicine composition for controlling porcine viral diseases and preparation method thereof
CN104161817A (en) * 2013-08-30 2014-11-26 郑州后羿制药有限公司 Compound traditional Chinese medicine composition for preventing and treating chicken bacteriosis and preparation method thereof
CN110812407A (en) * 2019-11-22 2020-02-21 佛山市正典生物技术有限公司 Sihuang dysentery stopping micro-capsule and preparation method thereof

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CN110812407A (en) * 2019-11-22 2020-02-21 佛山市正典生物技术有限公司 Sihuang dysentery stopping micro-capsule and preparation method thereof

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