CN103585578A - Traditional Chinese medicine for prevention and treatment of broiler chicken colibacillosis - Google Patents
Traditional Chinese medicine for prevention and treatment of broiler chicken colibacillosis Download PDFInfo
- Publication number
- CN103585578A CN103585578A CN201310541886.6A CN201310541886A CN103585578A CN 103585578 A CN103585578 A CN 103585578A CN 201310541886 A CN201310541886 A CN 201310541886A CN 103585578 A CN103585578 A CN 103585578A
- Authority
- CN
- China
- Prior art keywords
- radix
- parts
- chinese medicine
- broiler
- fructus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
The invention discloses a traditional Chinese medicine for prevention and treatment of broiler chicken colibacillosis. The traditional Chinese medicine is composed of the following drugs in parts by weight: 15-20 parts of coptis chinensis, 20-24 parts of radix pulsatilla, 15-20 parts of radix scutellariae, 20-28 parts of folium isatidis, 12-14 parts of pericarpium granati, 10-12 parts of fructus mume, 15-20 parts of fructus chebulae, 12-15 parts of radix astragali, 20-30 parts of hawthorn, 10-20 parts of medicated leaven, 20-22 parts of radix curcumae, and 15-18 parts of radix aucklandiae. The prescription is developed according to Chinese veterinarian overall concepts and a dialectic diagnosis and treatment theory, has heat-clearing, detoxifying, anti-bacterial, anti-inflammatory, intestine-astringing and antidiarrheal efficacies, and has remarkable effect on prevention and treatment of broiler chicken colibacillosis. Popularization application indicates that clinical treatment of broiler chicken colibacillosis has the cure rate of 84.5%. Preventive medication is implemented on broiler chicken farms, the broiler chicken colibacillosis death rate is decreased from 9.0% to 1.89%; the prescription has no residue, does not easily produce drug resistance, and allows meat products to be safe to eat; and the traditional Chinese medicine prescription is simple in preparing method, easy to use, and beneficial for popularization.
Description
Technical field
The present invention relates to chicken Chinese medicine, particularly a kind of Chinese medicine of preventing and treating broiler colibacillosis.
Background technology
Broiler colibacillosis is one of important epidemic disease of a kind of broiler of causing of dust Xi Shi escherichia coli different serotypes.Main manifestations is septicemia, fiber disposition airsacculitis, perihepatitis, pericarditis and peritonitis clinically.The chicken of each kind and age in days all can infect, but the easy infection of broiler, and this disease is broiler primary disease both, is often again the secondary disease in the various diseases generating processes such as newcastle, bird flu, chronic respiratory tract disease, and causes high M & M.
，Yu backlands district broiler breeding development in recent years very rapidly and intensive degree improve constantly, the important channel that has become important broiler export base and peasant employment and got rich.But because broiler chicks quality is uneven, the relatively lagging behind and the abuse of antimicrobial drug of feeding and management, cause the popular impetus of broiler colibacillosis to grow in intensity, develop in a healthy way to broiler breeding industry and bring serious threat.And drug residue and Amphixenosis also make this disease have important public health meaning.Chinese herbal medicine and Chinese medicinal formulae have good prevention effect to broiler colibacillosis, have the advantages such as the low and difficult generation drug resistance of residual hazard, meet the theme of green cultivation.Therefore, traditional Chinese medicine is paid attention to again by people, and in poultry Prevention of Infectious Diseases, bringing into play is more and more important effect, has vast potential for future development.The present invention is theoretical according to Chinese veterinarian's organic conception and determination for the treatment of based on pathogenesis obtained through differentiation of symptoms and signs, develops a kind of control broiler colibacillosis compound Chinese medicinal preparation, and being intended to provides new thinking for clinical prevention broiler colibacillosis.
At present, clinical common antibiotics and other chemical antibacterials are treated broiler colibacillosis, cause cause of disease drug resistance to be on the rise.Drug residue is detrimental to health and is subject to strong interest and query.And adopt immunity inoculation prevention broiler colibacillosis, and because colibacillary serotype is very many, use vaccine immunization effect undesirable, often cause immuning failure.In recent years, although Chinese medicine has been applied to treat chicken colibacillosis, special-purpose control broiler colibacillosis Chinese medicinal formulae is also rare.
Summary of the invention
The problem existing for overcoming above-mentioned prior art, the invention provides a kind of Chinese medicine of preventing and treating broiler colibacillosis, adopt this prescription outstanding to broiler colibacillosis prevention effect, cure rate is high, be used for preventing broiler colibacillosis, can improve broiler survival rate, apply and show: clinical treatment broiler colibacillosis, cure rate reaches 84.5%.Preventive usage is carried out in broiler field, and broiler colibacillosis mortality rate drops to 1.89% by 9.0%; Drug residue free, be difficult for to produce drug resistance, and this Chinese medicine preparation advantages such as to have preparation method simple, easy to use.
For achieving the above object, technical scheme of the present invention is:
Prevent and treat the colibacillary Chinese medicine of broiler, by Rhizoma Coptidis, the Radix Pulsatillae, Radix Scutellariae, Folium Isatidis, Pericarpium Granati, Fructus Mume, Fructus Chebulae, the Radix Astragali, Fructus Crataegi, Massa Medicata Fermentata, Radix Curcumae and the Radix Aucklandiae, formed.
Further, each component of above-mentioned Chinese medicine is counted by weight: Rhizoma Coptidis 15～20, the Radix Pulsatillae 20～24, Radix Scutellariae 15～20, Folium Isatidis 20～28, Pericarpium Granati 12～14, Fructus Mume 10～12, Fructus Chebulae 15～20, the Radix Astragali 12～15, Fructus Crataegi 20～30, Massa Medicata Fermentata 10～20, Radix Curcumae 20～22, the Radix Aucklandiae 15～18.
Further, each component of above-mentioned Chinese medicine is counted by weight: Rhizoma Coptidis 15, the Radix Pulsatillae 20, Radix Scutellariae 15, Folium Isatidis 20, Pericarpium Granati 12, Fructus Mume 10, Fructus Chebulae 15, the Radix Astragali 12, Fructus Crataegi 20, Massa Medicata Fermentata 10, Radix Curcumae 20 and the Radix Aucklandiae 15.
Further, describedly for preventing and treating the preparation method of the Chinese medicine of broiler colibacillosis, be:
(1) take by weight each component;
(2) add 8 times of quality of distilled water, soak 35min, decoction, boil rear 1h at a simmer, by 4 layers of filtered through gauze;
(3) medicinal residues add appropriate boiling distillated water, decoct 30min, filter;
(4) merging twice medicinal liquid, by rotary evaporator, to be concentrated into crude drug content in medicinal liquid be 0.5g/mL, is placed in 4 ℃ of Refrigerator stores, standby.
Further, describedly for preventing and treating the preparation method of the Chinese medicine of broiler colibacillosis, be:
(1) take by weight Rhizoma Coptidis 15, the Radix Pulsatillae 20, Radix Scutellariae 15, Folium Isatidis 20, Pericarpium Granati 12, Fructus Mume 10, Fructus Chebulae 15, the Radix Astragali 12, Fructus Crataegi 20, Massa Medicata Fermentata 10, Radix Curcumae 20 and the Radix Aucklandiae 15 raw materials;
(2) above-mentioned raw materials is mixed, with plant pulverizer, pulverizes, crosses 80～100 mesh sieves, be distributed in 500g/ bag, seal standby.
Composition principle of the present invention is:
Chinese veterinarian's determination for the treatment of based on pathogenesis obtained through differentiation of symptoms and signs thinks, broiler colibacillosis is by the invasion of epidemic disease poison, and damp and hot heap soil or fertilizer over and around the roots is amassed intestinal, exuberant lung-stomach heat, the blood stasis stagnation of QI and caused.Escherichia coli are invaded after chicken body, fervescence, and spirit is withered blunt, has asthma and diarrhoea performance, and colibacillosis is like mental confusion due to phlegm and diarrheal disease, and method is when having one's ideas straightened out expectorant, relieving diarrhea with astringents.Treat the Chinese medicine of appropriate to the occasion selection heat-clearing and toxic substances removing, promoting blood circulation to remove blood stasis, relieving diarrhea with astringents, this test is carried out prescription according to this viewpoint just, the compound preparation of organizing has stronger heat-clearing and toxic substances removing and bactericidal action, various biological functions that can also comprehensively regulating body, enhancing human body immunity power.
Theoretical according to Chinese veterinarian, to the control of broiler colibacillosis, should follow that heat-clearing and toxic substances removing, dampness removing heat from blood, astringing intestine to stop diarrhea promote the production of body fluid, the principle of invigorating the spleen and regulating the stomach, to reach the effect of eliminating pathogenic factor for supporting vital QI.
According to above-mentioned theory and principle, the invention provides a kind of for preventing and treating the colibacillary Chinese drug-treated group of broiler and prescription.
Rhizoma Coptidis: hardship, cold.GUIXIN, spleen, stomach, liver, gallbladder, large intestine channel.Rhizoma Coptidis is famous Chinese medicine, < < Sheng Nong's herbal classic > > row be top grade.Heat clearing and damp drying, eliminating fire and detoxication.For damp and hot feeling of fullness, vomiting, dysentery, jaundice, unconsciousness due to high fever, hyperactivity of heart-fire, dysphoria and insomnia, heat in blood is told nosebleed, conjunctival congestion acid regurgitation, toothache, quenches one's thirst, carbuncle furuncle; External treatment eczema, eczema, auditory meatus is suppurated.It has inhibitory action to many pathogenic bacterias such as shigella dysenteriae, staphylococcus aureus, salmonella typhi, vibrio cholera, and bacillary dysentery is had to significant curative effect.Rhizoma Coptidis can be eliminated colibacillary R plasmid, and the drug resistance of fluoroquinolone antibacterial agent, gentamycin and doxycycline is had to obvious inhibitory action.
The Radix Pulsatillae: hardship, cold.Enter large intestine, liver, stomach warp.Heat-clearing and toxic substances removing, the effect of eliminating pathogenic heat from blood to cure dysentery.Be good at the damp and hot and noxious heat in the blood system of clearing stomach intestinal, therefore for controlling the good medicine of toxic-heat and blood stasis.With controlling hematodiarrhoea stomachache, tenesmus, dysentery pus and blood, can be alone, or compatibility Rhizoma Coptidis.This product is available peptic ulcer, neurodermatitis, acute pyelonephritis, necrotic enteritis, the dysfunctional uterine hemorrhage etc. controlled still.The Radix Pulsatillae, to staphylococcus aureus, bacillus pyocyaneus, dysentery bacterium, bacillus subtilis, Bacillus typhi, Salmonella and some dermatophytess etc., all has obvious inhibitory action.
Radix Scutellariae: bitter in the mouth, cold in nature.Enter lung, gallbladder, large intestine channel, have heat clearing and damp drying, eliminating fire and detoxication, antiabortive effect, be mainly used in damp and hot due to abdomen dysentery, jaundice, pyretic stranguria; Also can be used for excessive fetal movement due to heat in pregnancy, pyretic toxicity pathogenic fire is invaded the swollen card of carbuncle sore poison of excessive skin.Radix Scutellariae has certain inhibitory action to staphylococcus, blood dissolubility streptococcus, Diplococcus pneumoniae, Bacillus typhi, dysentery coccus, escherichia coli, bacillus pyocyaneus, colibacillary R plasmid is had to higher elimination factor, thereby can eliminate the colibacillary drug resistance of plasmid antagonism of Resistant strain, baicalin also can reduce escherichia coli endotoxin content, alleviates the damage of endotoxin to the damage of internal organs and endotoxin Human Umbilical Vein Endothelial Cells membrane structure.
Folium Isatidis: hardship, cold.Enter liver, the heart, stomach warp.Heat clearing away, removing toxic substances, removing heat from blood, hemostasis.Control epidemic febrile disease intenseness of heat excessive thirst, influenza, acute infectious hepatitis, bacillary dysentery, acute gastroenteritis, acute pneumonia, erysipelas, spits blood, epistaxis, jaundice, dysentery, sore throat, aphtha, swollen ulcer drug.Staphylococcus aureus, alpha streptococcus, meningococcus, streptococcus pneumoniae, micrococcus catarrhalis, Bacillus typhi, escherichia coli, hemophilus influenza, diphtheria corynebacterium and dysentery bacterium are had to certain inhibitory action.Experiment in vivo and vitro shows that Folium Isatidis has the enterobacteria O of the Chinese People's Anti-Japanese Military and Political College
111the effect of B4 endotoxin.
Pericarpium Granati: cold in nature, sour-puckery flavor.Enter large intestine, kidney two warps.Relieving diarrhea with astringents, hemostasis, anthelmintic, dysentery, discharging fresh blood stool.Treatment chronic diarrhea, chronic dysentery, has blood in stool, proctoptosis.There is astriction, after it contacts with gastrointestinal mucosa, can precipitate or solidify local protein, make on surface, to form comparatively fine and close protective layer, contribute to local woanded surface healing or protection part to avoid stimulating.Experiment in vitro proof all has inhibitory action to various bacteria such as tubercule bacillus, escherichia coli, Bacillus proteus, bacillus pyocyaneus.
Fructus Chebulae: bitter in the mouth, acid, puckery, property is flat.Enter lung, large intestine channel.Convergence, anti-diarrhea effect.Be applicable to the symptom of chronic diarrhea chronic dysentery.Various dysentery bacteriums are all had to inhibitory action, stronger to the effect of bacillus pyocyaneus, diphtheria corynebacterium, staphylococcus aureus, escherichia coli, streptococcus pneumoniae, Hemolytic streptococcus, Bacillus proteus, bacillus typhi murium are also had to inhibitory action.
Fructus Mume: acid, puckery, flat.Return liver, spleen, lung, large intestine channel.Astringe the lung, astringing intestine to stop diarrhea, promotes the production of body fluid, anthelmintic.For chronic diarrhea, dysentery, has blood in stool etc.Normal and small-mouthed jar baby shell, Fructus Chebulae are equal to use.Experiment in vitro shows: to having inhibitory action in vitro as to anthrax bacillus, staphylococcus, streptococcus pneumoniae, to equal effective inhibitory action such as escherichia coli, dysentery bacterium, Typhoid and paratyphoid bacillus.
Radix Curcumae: pungent, hardship, cold.Return liver, the heart, lung meridian.Regulating qi to disperse stagnation, heart fire-clearing upset-relieving, promoting the function of the gallbladder to alleviate jaundice.For chest and abdomen swelling and pain, jaundice dark coloured urine.Radix Curcumae polysaccharide has immunocompetence, can strengthen the activity of reticuloendothelial system, improves immunity.
The Radix Aucklandiae: acrid in the mouth, micro-hardship, warm in nature.Enter spleen, stomach, large intestine, gallbladder meridian.The function of promoting the flow of QI for relieving the stagnancy, stomach function regulating antidiarrheal.For row flatulence, therefore the diseases such as all dyspepsias, loss of appetite, abdominal distention distending pain are all effective.The pharmacological research Radix Aucklandiae has inhibitory action to escherichia coli, dysentery bacterium and Bacillus typhi.
The Radix Astragali: sweet, tepor.Return lung, spleen, liver, kidney channel.The Radix Astragali has the effect that benefiting QI for strengthening the superficies, arresting sweating are admittedly de-, hold in the palm skin ulcer granulation promoting, inducing diuresis to remove edema.Be used for the treatment of the deficiency of vital energy weak, sinking of QI of middle-JIAO, chronic diarrhea proctoptosis, the metrorrhagia of having blood in stool, exterior deficiency spontaneous perspiration, carbuncle is difficult bursts, and burst and do not hold back for a long time, blood deficiency and yellow complexion, interior-heat is quenched one's thirst.There is invigorating the spleen and replenishing QI effect with other medicines compatibility, treatment weakness of the spleen and stomach, inappetence, anorexia and loose stool.Modern pharmacology shows, the Radix Astragali significantly enhancing non-specific immunity function and different in nature immunologic function, increase RBC number, improve immunity, the Radix Astragali has inhibitory action to kinds of experiments animal gastric ulcer, and can work in coordination with cimetidine gastric mucosa is had to protective effect.
Fructus Crataegi: sour in the mouth, sweet, slightly warm in nature.Return spleen, stomach, Liver Channel.The effects such as that removing food stagnancy is spent is stagnant, convergence dysentery relieving, activating blood circulation to dissipate blood stasis.Be used for appetite-stimulating indigestion-relieving, change stagnant removing food stagnancy, promoting blood circulation to remove blood stasis, the circulation of qi promoting of reducing phlegm.
Massa Medicata Fermentata: sweet, acrid, warm.Enter spleen, stomach warp.Invigorating the spleen and regulating the stomach, the effect helping digestion in adjusting.Control retention of food and drink, feeling of stuffiness in chest abdominal distention, vomiting dysentery, dyspepsia.
Chinese medicinal formulae of the present invention to the mechanism of action of control broiler colibacillosis is:
In Chinese prescription of the present invention, Rhizoma Coptidis, Radix Scutellariae, Folium Isatidis, the Radix Pulsatillae have heat-clearing and toxic substances removing, dampness and removing heat from blood function.Pharmacological research shows, these Chinese medicines have the effect of bacteriostasis and sterilization to chicken colibacillosis, and use in conjunction effect is better; The Radix Astragali, Radix Curcumae play QI invigorating and consolidate, improve immunity of organisms, reach antibiotic object, and this also plays very pivotal role in broiler colibacillosis control.
Broiler Drug Resistance of E. coli is the difficult problem that antibiotic and chemical antimicrobial drug are difficult to overcome, by adding Rhizoma Coptidis, Radix Scutellariae, Folium Isatidis, higher to the clearance rate of colibacillary R-plasmid in Chinese prescription of the present invention.The R-plasmid that can both obviously strengthen antibacterial as Rhizoma Coptidis and Radix Scutellariae compatibility, Radix Scutellariae and the Radix Pulsatillae is eliminated, and after Rhizoma Coptidis and Radix Scutellariae compatibility, 48h can reach 26.1% to the elimination factor of plasmid.Thereby can eliminate the drug resistance of Resistant strain.
Chinese medicinal components of the present invention has the endotoxic effect of Chinese People's Anti-Japanese Military and Political College enterobacteria.Chicken colibacillosis endotoxin is its important virulence factor causing a disease, and can cause the damage of multiple organ, by adding Rhizoma Coptidis, Radix Scutellariae and Folium Isatidis can directly remove escherichia coli endotoxin.
Chinese medicinal components of the present invention has relieving diarrhea with astringents function.In Chinese prescription, add Pericarpium Granati, Fructus Mume and Fructus Chebulae, play relieving diarrhea with astringents, promoting the production of body fluid to quench thirst, convergence and protection gastrointestinal mucosa, prevent dehydration.Also have colibacillary inhibitory action simultaneously.
In Chinese medicinal formulae of the present invention, by adding Fructus Crataegi, Massa Medicata Fermentata, have and regulate the flow of vital energy, the effect of invigorating the spleen and regulating the stomach, can facilitating digestion, eliminates eating accumulation, in case distension and fullness in the abdomen promotes chicken food to want to increase, the function of putting on weight.
With respect to prior art, beneficial effect of the present invention is:
This prescription is to develop according to Chinese veterinarian's organic conception and determination for the treatment of based on pathogenesis obtained through differentiation of symptoms and signs theory, and control broiler colibacillosis effect is remarkable, applies and shows: clinical treatment broiler colibacillosis, cure rate reaches 84.5%.Preventive usage is carried out in broiler field, and broiler colibacillosis mortality rate drops to 1.89% by 9.0%; Noresidue, difficult drug resistance, the meat product edible safety of producing; This Chinese medicinal formulae preparation method is simple, easy to use, is beneficial to popularization.
The specific embodiment
Below in conjunction with specific embodiment, the present invention is described in further details:
Embodiment 1 Chinese medicinal formulae of the present invention is to the therapeutic test of artificial challenge's E.coli disease in boiler
Experiment material
Chinese herbal medicine: Rhizoma Coptidis, the Radix Pulsatillae, Radix Scutellariae, Folium Isatidis, Pericarpium Granati, Fructus Mume, Fructus Chebulae, the Radix Astragali, Fructus Crataegi, Massa Medicata Fermentata, Radix Curcumae, the Radix Aucklandiae, all purchased from Anyang Affiliated Hospital of Chinese Medical School.Above-mentioned prescription Chinese medicine meets the relevant regulations of < < Chinese veterinary pharmacopoeia > >.
Antibiotic: 30% florfenicol injection, specification 10mg/ml, Shandong Qilu Pharmaceutical Factory produces.During use, carry out 10 times of dilutions.
Culture medium: broth bouillon is pressed the preparation of reference method, and maconkey agar culture medium is commercial goods.
Bacterial strain: by the Henan Bei Roujiyangzhichang separated escherichia coli in Broiler Chicken that die of illness, through this laboratory qualification, serotype is O
1, O
2, O
74type bacterial strain, preserves at this laboratory.
Experimental animal: the assorted chicken of 1 age in days 817 meat is provided by hatchery, suburb, Anyang, raises the experimental animal room in biological institute of Anyang Institute of Technology.Its feedstuff of duration of test is prepared production voluntarily, and or not containing the mixed feed of any antimicrobial drug, white mice is not purchased from Anyang City pharmaceutical factory.
Test procedure
Chinese medicinal formulae preparation: take by weight Rhizoma Coptidis 15, the Radix Pulsatillae 20, Radix Scutellariae 15, Folium Isatidis 20, Pericarpium Granati 12, Fructus Mume 10, Fructus Chebulae 15, the Radix Astragali 12, Fructus Crataegi 20, Massa Medicata Fermentata 10, Radix Curcumae 20 and the Radix Aucklandiae 15; Add 8 times of amounts of distilled water, soak 35min, decoction, boil rear 1h at a simmer, by 4 layers of filtered through gauze; Medicinal residues add appropriate boiling distillated water, decoct 30min, filter; Merging twice medicinal liquid, by rotary evaporator, to be concentrated into crude drug content in medicinal liquid be 0.5g/mL.Be placed in 4 ℃ of Refrigerator stores, standby
The preparation of test organisms suspension: by the O preserving
1, O
2, O
74type bacterial strain is inoculated into respectively on maconkey agar flat board, cultivates 24h for 37 ℃.Select pinkiness protuberance, ganoid bacterium colony, inoculate fresh broth bouillon, cultivate 18h for 37 ℃, after pure inspection, inoculation Nutrient agar, cultivates 20h, puts room temperature and soak 8min with sterile saline for 37 ℃, scrape lawn, with the husky cloth of sterilizing, be filled in sterilizing test tubes, by nephelometer, count method counting, being mixed with bacteria concentration is the bacterium liquid of 109CFU/ml, then 3 strain bacterium liquid equal proportions are mixed, and 4 ℃ of Refrigerator stores are standby.
Test organisms pathogenicity: getting body weight is 8 of 15～17g healthy mices, random packet, 4/group, only, matched group same method inoculation normal saline, observes morbidity and death condition to test group intraperitoneal mouse inoculation mixed bacteria liquid 0.2ml/.
Test grouping: broiler chicken is raised 21 ages in days, and chicken health, without Novel presentation, is then divided into 4 groups, 30/group at random.
Except normal healthy controls group, 0.25ml/ counteracting toxic substances of all the other 3 groups of broiler chicken chest muscle injection mixed bacteria liquids.When broiler chicken occurs after symptom, treatment by Chinese herbs test group chicken gavages 1.0ml/ traditional Chinese medicine decoction immediately, every morning, afternoon each 1 time, be used in conjunction 5d, florfenicol 1ml/ after the cervical region subcutaneous injection dilution immediately of florfenicol treatment group chicken only, the morning, afternoon each 1 time, be used in conjunction 5d, and normal healthy controls group and infection matched group give normal saline (1ml/ only).Experimental observation 14d.Record the clinical symptoms change of broiler chicken, dead chicken is cutd open to inspection, carry out antibacterial separation and Culture, to determine the cause of the death.And the protective rate of broiler chicken is respectively organized in calculating.
1. therapeutic evaluation infects matched group broiler hyponoia at duration of test, wing hangs down, slow-witted vertical, poor appetite, has loose bowels and dyspnea, die of illness after chicken necropsy, pericardium and liver have faint yellow cellulose membrane, and heart wall attenuation, pericardial fluid increase, and have the typical particular-trade sexually transmitted disease (STD)s such as cellulose oozes out, air bag muddiness to become, and isolate corresponding escherichia coli, judgement coli-infection is dead.2. significance test of data is checked with t.
Experimental result
The pathogenicity test results of test organisms: test group white mice is all dead successively in inoculation test bacterium mixed liquor 3d, cuts open the dead Mus of inspection, and visceral pericardium is hemorrhage, hepatomegaly, blackout or hemorrhage.From dead Liver of Mice, ascites, escherichia coli have been recovered to.4 white mice health of matched group, illustrate that test organisms mixed liquor has very strong pathogenic.
The treatment experimental result of Chinese medicinal formulae of the present invention to artificial challenge's broiler colibacillosis:
As seen from Table 1; compound Chinese medicinal preparation and florfenicol all can significantly reduce the mortality rate of artificial challenge escherichia coli broiler; the escherichia coli protective rate for the treatment of by Chinese herbs test group is 86.6%, and florfenicol group is 83.3%, and infecting matched group conservation of nature rate is 26.6%.Result shows that this Chinese medicinal formulae therapeutic effect is remarkable.
The treatment experimental result of table 1 prescription preparation to tentative broiler colibacillosis
Note: * represents to compare with infection matched group difference extremely significantly (P<0.01).
Embodiment 2 Chinese medicinal formulae of the present invention is to artificial challenge's E.coli disease in boiler prophylactic tria
Experiment material
Chinese herbal medicine: Rhizoma Coptidis, the Radix Pulsatillae, Radix Scutellariae, Folium Isatidis, Pericarpium Granati, Fructus Mume, Fructus Chebulae, the Radix Astragali, Fructus Crataegi, Massa Medicata Fermentata, Radix Curcumae, the Radix Aucklandiae, all purchased from Anyang Affiliated Hospital of Chinese Medical School.Above-mentioned prescription Chinese medicine meets the relevant regulations of < < Chinese veterinary pharmacopoeia > >.
Antibiotic: 30% florfenicol injection, specification 10mg/ml, Shandong Qilu Pharmaceutical Factory produces.During use, carry out 10 times of dilutions.
Culture medium: broth bouillon is pressed the preparation of reference method, and maconkey agar culture medium is commercial goods.
Bacterial strain: by the Henan Bei Roujisiyangchang separated escherichia coli in Broiler Chicken that die of illness, through this laboratory qualification, serotype is O
1, O
2, O
74type bacterial strain, preserves at this laboratory.
Experimental animal: the assorted chicken of 1 age in days 817 meat is provided by hatchery, suburb, Anyang, raises the experimental animal room in biological institute of Anyang Institute of Technology.Its feedstuff of duration of test is prepared production voluntarily, and or not containing the mixed feed of any antimicrobial drug, white mice is not purchased from Anyang City pharmaceutical factory.
Experimental procedure
Chinese medicinal formulae preparation: take by weight Rhizoma Coptidis 15, the Radix Pulsatillae 20, Radix Scutellariae 15, Folium Isatidis 20, Pericarpium Granati 12, Fructus Mume 10, Fructus Chebulae 15, the Radix Astragali 12, Fructus Crataegi 20, Massa Medicata Fermentata 10, Radix Curcumae 20 and the Radix Aucklandiae 15; Add 8 times of amounts of distilled water, soak 35min and decoct, boil rear 1h at a simmer, by 4 layers of filtered through gauze; Medicinal residues add appropriate boiling distillated water, decoct 30min, filter; Merging twice medicinal liquid, by rotary evaporator, to be concentrated into crude drug content in medicinal liquid be 0.5g/mL.Be placed in 4 ℃ of Refrigerator stores, standby
The preparation of test organisms suspension: by the O preserving
1, O
2, O
74type bacterial strain is inoculated into respectively on maconkey agar flat board, cultivates 24h for 37 ℃.Select pinkiness protuberance, ganoid bacterium colony, inoculate fresh broth bouillon, cultivate 18h for 37 ℃, after pure inspection, inoculation Nutrient agar, cultivates 20h, puts room temperature and soak 8min with sterile saline for 37 ℃, scrape lawn, with the husky cloth of sterilizing, be filled in sterilizing test tubes, by nephelometer, count method counting, being mixed with bacteria concentration is the bacterium liquid of 109CFU/ml, then 3 strain bacterium liquid equal proportions are mixed, and 4 ℃ of Refrigerator stores are standby.
Test organisms pathogenicity: getting body weight is 8 of 15～17g healthy mices, random packet, 4/group, only, matched group same method inoculation normal saline, observes morbidity and death condition to test group intraperitoneal mouse inoculation mixed bacteria liquid 0.2ml/.
Test grouping: broiler chicken is raised 21 ages in days, and chicken health, without Novel presentation, is then divided into 4 groups, 30/group at random.
Chinese herb on the prevention test group: broiler chicken 21 ages in days start gavages 0.5ml/ traditional Chinese medicine decoction at every turn, the morning, afternoon each 1 time, gavages continuously 10d; Florfenicol prophylactic tria group: broiler chicken is after 24 ages in days, and the florfenicol 0.5ml/ after the dilution of cervical region subcutaneous injection only, the morning, afternoon each 1 time, injects 7d continuously; Infect matched group: 21 ages in days give normal saline (1ml/ only), the morning, afternoon each 1 time, gavage continuously 10d.Chinese herb on the prevention test group gavages the 4d after Chinese medicine, 2d and 0.25ml/ counteracting toxic substances of infection matched group while chest muscle injection mixed bacteria liquid of florfenicol prophylactic tria group injection florfenicol, and normal healthy controls group is counteracting toxic substances not, not medicine feed.Observe 14d, record the clinical symptoms change of broiler chicken, dead chicken is cutd open to inspection, carry out antibacterial separation and Culture, to determine the cause of the death.And the protective rate of broiler chicken is respectively organized in calculating.
1. therapeutic evaluation infects matched group broiler hyponoia at duration of test, wing hangs down, slow-witted vertical, poor appetite, has loose bowels and dyspnea, die of illness after chicken necropsy, pericardium and liver have faint yellow cellulose membrane, and heart wall attenuation, pericardial fluid increase, and have the typical particular-trade sexually transmitted disease (STD)s such as cellulose oozes out, air bag muddiness to become, and isolate corresponding escherichia coli, judgement coli-infection is dead.2. significance test of data is checked with t.
Experimental result
The pathogenicity of test organisms: test group white mice is all dead successively in inoculation test bacterium mixed liquor 3d, cuts open the dead Mus of inspection, and visceral pericardium is hemorrhage, hepatomegaly, blackout or hemorrhage.From dead Liver of Mice, ascites, escherichia coli have been recovered to.4 white mice health of matched group, illustrate that test organisms mixed liquor has very strong pathogenic.
The preventive experiment result of Chinese medicinal formulae of the present invention to artificial challenge's broiler colibacillosis:
From table 2, Chinese herbal compound preparation has good protective effect to prevention broiler colibacillosis, and protective rate is 93.3%, identical with the protective rate of florfenicol, and has effect of gain.
The prophylactic tria result of table 2 prescription preparation to tentative broiler colibacillosis
Note: * represents to compare with infection matched group difference extremely significantly (P<0.01).
Embodiment 3 Chinese medicine preparation of the present invention is to clinical onset broiler treatment experiment
The broiler field of the districts and cities such as the Anyang in Yu backlands district, Puyang, Hebi, adopts the Chinese medicinal formulae of this development morbidity broiler to be carried out to the Clinical Treatment Test of colibacillosis.
Chinese medicinal formulae preparation: take by weight Rhizoma Coptidis 15, the Radix Pulsatillae 20, Radix Scutellariae 15, Folium Isatidis 20, Pericarpium Granati 12, Fructus Mume 10, Fructus Chebulae 15, the Radix Astragali 12, Fructus Crataegi 20, Massa Medicata Fermentata 10, Radix Curcumae 20 and the Radix Aucklandiae 15; Add 8 times of amounts of distilled water, soak 35min, decoction, boil rear 1h at a simmer, by 4 layers of filtered through gauze; Medicinal residues add appropriate boiling distillated water, decoct 30min, filter; Merging twice medicinal liquid, by rotary evaporator, to be concentrated into crude drug content in medicinal liquid be 0.5g/mL.Bottling is placed in 4 ℃ of Refrigerator stores, standby
Medication: adopt and gavage method administration.To there is the broiler of colibacillosis, occur after symptom, gavage immediately Chinese medicine preparation of the present invention, every day at the upper and lower noon each once, each consumption 1.0mL/kg, 6d medication continuously.
Clinical treatment the results are shown in Table 3.
From table 3,1.06 ten thousand of clinical treatment broiler colibacillosis, average cure rate 84.5%.Cure rate a little less than artificial challenge's broiler colibacillosis, this may natural production condition with in experiment condition difference, cause, but still higher than the cure rate to the florfenicol 83.3% of escherichia coli sensitivity under experiment condition, result also shows, this Chinese medicinal formulae also has good curative effect to the broiler colibacillosis of natural infection.
Table 3 prescription preparation for treating broiler colibacillosis clinical application effect
Embodiment 3 Chinese medicine preparation of the present invention is to preventing broiler colibacillosis experiment in producing
Experimental procedure
Backlands district, Henan does not carry out the sick Epidemiological study of colibacillosis preventive usage broiler sickness rate: take the method with broiler breeding field poultry raiser, veterinary's informal discussion, inspection information, colibacillosis Epidemiological study is carried out in Anyang, Hebi and Puyang San Ge districts and cities part broiler field, comprise that the indexs such as morbidity broiler field scale, kind, quantity, sickness rate and case fatality rate investigate.
Chinese medicinal formulae preparation: take by weight Rhizoma Coptidis 15, the Radix Pulsatillae 20, Radix Scutellariae 15, Folium Isatidis 20, Pericarpium Granati 12, Fructus Mume 10, Fructus Chebulae 15, the Radix Astragali 12, Fructus Crataegi 20, Massa Medicata Fermentata 10, Radix Curcumae 20 and the Radix Aucklandiae 15 raw materials; Above-mentioned raw materials is mixed, with plant pulverizer, pulverizes, crosses 80～100 mesh sieves, be distributed in 500g/ bag, seal standby.
The Chinese medicine preparation of above-mentioned preparation is fully mixed with feedstuff, by the administration of broiler 0.5g/kg/d amount, after feeding of broiler 23 ages in days, start preventive usage, successive administration 10d.
To not carrying out the survey result of colibacillosis preventive usage broiler sickness rate: to 3 districts and cities, 145 chicken groups, 121.95 ten thousand investigation.Survey result, the average mortality 9.0% of broiler colibacillosis.Result shows, broiler colibacillosis is very serious to broiler harm.
Broiler colibacillosis preventive experiment result in production
The broiler field, many, Chinese medicine formula Dui Yu backlands district of this development and poultry specialist, carry out broiler colibacillosis prevention, and effect is remarkable.From table 4, after 125.19 ten thousand broiler preventive usages, average mortality is reduced to 1.89%.And in the Epidemiological study of before 121.95 ten thousand broiler being carried out, the average mortality 9.0% of colibacillosis.Result shows, this Chinese medicinal formulae prevention broiler colibacillosis effect is more remarkable.
Applying of table 4 prescription preparation prevention broiler colibacillosis
This Chinese medicinal components aboundresources, cheap, convenient drug administration, allows chicken free choice feeding, and not only reach therapeutic purposes, and can prevent well the generation of chicken colibacillosis, be a kind of desirable novel Chinese herbal and crude drugs preparations that has no side effect.Can replace that to take at present antibiotic be the main colibacillary way of prophylactic treatment broiler completely, solve the problem of the residual and drug resistance of antibiotic medicine, produce non-harmful green product.
The above embodiment of the present invention is only for example of the present invention is clearly described, and is not the restriction to embodiments of the present invention.For those of ordinary skill in the field, can also make on the basis of the above description other multi-form variation and changes.Here cannot give all embodiments exhaustive.Every still row in protection scope of the present invention of apparent variation that technical scheme of the present invention amplifies out or change that belong to.
Claims (5)
1. for preventing and treating the colibacillary Chinese medicine of broiler, by Rhizoma Coptidis, the Radix Pulsatillae, Radix Scutellariae, Folium Isatidis, Pericarpium Granati, Fructus Mume, Fructus Chebulae, the Radix Astragali, Fructus Crataegi, Massa Medicata Fermentata, Radix Curcumae and the Radix Aucklandiae, formed.
2. Chinese medicine as claimed in claim 1, it is characterized in that, each component is counted by weight: Rhizoma Coptidis 15～20, the Radix Pulsatillae 20～24, Radix Scutellariae 15～20, Folium Isatidis 20～28, Pericarpium Granati 12～14, Fructus Mume 10～12, Fructus Chebulae 15～20, the Radix Astragali 12～15, Fructus Crataegi 20～30, Massa Medicata Fermentata 10～20, Radix Curcumae 20～22, the Radix Aucklandiae 15～18.
3. Chinese medicine as claimed in claim 2, is characterized in that, each component is counted by weight: Rhizoma Coptidis 15, the Radix Pulsatillae 20, Radix Scutellariae 15, Folium Isatidis 20, Pericarpium Granati 12, Fructus Mume 10, Fructus Chebulae 15, the Radix Astragali 12, Fructus Crataegi 20, Massa Medicata Fermentata 10, Radix Curcumae 20 and the Radix Aucklandiae 15.
4. the Chinese medicine as described in as arbitrary in claim 1-3, is characterized in that, describedly for preventing and treating the preparation method of the Chinese medicine of broiler colibacillosis, is:
(1) take by weight each component;
(2) add 8 times of quality of distilled water, soak 35min, decoction, boil rear 1h at a simmer, by 4 layers of filtered through gauze;
(3) medicinal residues add appropriate boiling distillated water, decoct 30min, filter;
(4) merging twice medicinal liquid, by rotary evaporator, to be concentrated into crude drug content in medicinal liquid be 0.5g/mL, is placed in 4 ℃ of Refrigerator stores, standby.
5. the Chinese medicine as described in as arbitrary in claim 1-3, is characterized in that, describedly for preventing and treating the preparation method of the Chinese medicine of broiler colibacillosis, is:
(1) take by weight Rhizoma Coptidis 15, the Radix Pulsatillae 20, Radix Scutellariae 15, Folium Isatidis 20, Pericarpium Granati 12, Fructus Mume 10, Fructus Chebulae 15, the Radix Astragali 12, Fructus Crataegi 20, Massa Medicata Fermentata 10, Radix Curcumae 20 and the Radix Aucklandiae 15 raw materials;
(2) above-mentioned raw materials is mixed, with plant pulverizer, pulverizes, crosses 80～100 mesh sieves, be distributed in 500g/ bag, seal standby.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310541886.6A CN103585578A (en) | 2013-11-06 | 2013-11-06 | Traditional Chinese medicine for prevention and treatment of broiler chicken colibacillosis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310541886.6A CN103585578A (en) | 2013-11-06 | 2013-11-06 | Traditional Chinese medicine for prevention and treatment of broiler chicken colibacillosis |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103585578A true CN103585578A (en) | 2014-02-19 |
Family
ID=50076031
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310541886.6A Pending CN103585578A (en) | 2013-11-06 | 2013-11-06 | Traditional Chinese medicine for prevention and treatment of broiler chicken colibacillosis |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103585578A (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104784309A (en) * | 2015-03-10 | 2015-07-22 | 李博 | Chinese medicinal composition for control of duck colibacillosis |
| CN106309752A (en) * | 2016-08-30 | 2017-01-11 | 河南大德广动物药业有限公司 | Traditional Chinese medicine composition for preventing and treating colibacillosis of livestock and poultry and preparation method of traditional Chinese medicine composition |
| CN107912640A (en) * | 2017-12-14 | 2018-04-17 | 阜阳市玉琪农业科技有限公司 | A kind of feed for preventing chicken dysentery and preparation method thereof |
| CN107981093A (en) * | 2017-12-15 | 2018-05-04 | 阜阳市玉琪农业科技有限公司 | A kind of feed for preventing E. coli isolated from ducks and preparation method thereof |
| CN108434369A (en) * | 2018-06-15 | 2018-08-24 | 吉林农业大学 | Treat the probiotics herbal mixture zymotic fluid of chicken colibacillosis |
| CN109568560A (en) * | 2019-01-28 | 2019-04-05 | 孟繁华 | A kind of preparation method of active compound that treating the infection of Escherichia coli persister |
| CN115721676A (en) * | 2022-08-05 | 2023-03-03 | 广西大学 | Method for preventing and controlling chicken colibacillosis based on main organic acid in plum syrup |
| CN115721676B (en) * | 2022-08-05 | 2024-04-26 | 广西大学 | Based on main organic acid in plum juice for preventing and controlling colibacillosis enteritis of chickens |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101856416A (en) * | 2010-06-03 | 2010-10-13 | 河北科技师范学院 | Chinese herbal medicament oral liquid for treating chicken colibacillosis |
-
2013
- 2013-11-06 CN CN201310541886.6A patent/CN103585578A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101856416A (en) * | 2010-06-03 | 2010-10-13 | 河北科技师范学院 | Chinese herbal medicament oral liquid for treating chicken colibacillosis |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104784309A (en) * | 2015-03-10 | 2015-07-22 | 李博 | Chinese medicinal composition for control of duck colibacillosis |
| CN106309752A (en) * | 2016-08-30 | 2017-01-11 | 河南大德广动物药业有限公司 | Traditional Chinese medicine composition for preventing and treating colibacillosis of livestock and poultry and preparation method of traditional Chinese medicine composition |
| CN107912640A (en) * | 2017-12-14 | 2018-04-17 | 阜阳市玉琪农业科技有限公司 | A kind of feed for preventing chicken dysentery and preparation method thereof |
| CN107981093A (en) * | 2017-12-15 | 2018-05-04 | 阜阳市玉琪农业科技有限公司 | A kind of feed for preventing E. coli isolated from ducks and preparation method thereof |
| CN108434369A (en) * | 2018-06-15 | 2018-08-24 | 吉林农业大学 | Treat the probiotics herbal mixture zymotic fluid of chicken colibacillosis |
| CN109568560A (en) * | 2019-01-28 | 2019-04-05 | 孟繁华 | A kind of preparation method of active compound that treating the infection of Escherichia coli persister |
| CN115721676A (en) * | 2022-08-05 | 2023-03-03 | 广西大学 | Method for preventing and controlling chicken colibacillosis based on main organic acid in plum syrup |
| CN115721676B (en) * | 2022-08-05 | 2024-04-26 | 广西大学 | Based on main organic acid in plum juice for preventing and controlling colibacillosis enteritis of chickens |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103585578A (en) | Traditional Chinese medicine for prevention and treatment of broiler chicken colibacillosis | |
| CN102805275B (en) | Feed and traditional Chinese medicine compound used for treating colibacillosis of chicken and preparation method of feed and traditional Chinese medicine compound | |
| CN102613413B (en) | Feed and traditional Chinese medicine for treating white scour of piglets and preparation method as well as applications | |
| CN102552656B (en) | Traditional Chinese medicine composition for treating chicken colibacillosis | |
| CN103830404A (en) | Traditional Chinese medicine composition for treating Chicken colibacillosis, preparation method of traditional Chinese medicine mixture and traditional Chinese medicine mixture | |
| CN102847017B (en) | Oral solution for prevention and treatment of piglet diarrhea, and preparation method thereof | |
| CN102872392A (en) | Chinese medicinal preparation for preventing and controlling swine mycoplasma pneumonia and preparation method thereof | |
| CN102920957A (en) | Chinese herbal medicine additive for preventing pig salmonellosis and pig feed using additive | |
| CN103239651B (en) | Traditional Chinese medicine for treating subclinical mastitis for dairy cow | |
| CN102526510B (en) | Chinese composition for preventing and treating duck virus hepatitis | |
| CN104208239A (en) | Traditional Chinese medicine composition for preventing and treating poultry escherichia coli disease, microecological preparation, and preparation method | |
| CN105833185A (en) | Traditional Chinese medicine composition for preventing and treating colibacillosis of domestic rabbits | |
| CN103405628A (en) | Traditional Chinese medicine oral liquid for treating respiratory diseases of livestock and poultry | |
| CN103211985B (en) | Traditional Chinese medicine for treating infectious serositis of duck | |
| CN104474104A (en) | Traditional Chinese medicine composition for preventing and treating infectious rhinitis of chicken and preparation method thereof | |
| CN104547126A (en) | Traditional Chinese medicine composition for treating bacterial diarrhea of poultry | |
| CN106421176A (en) | Traditional Chinese medicine powder for preventing and curing weaned piglet diarrhea | |
| CN103830616A (en) | A traditional Chinese medicine for treating swine fever and a preparation method thereof | |
| CN106421037A (en) | Traditional Chinese medicine composition for preventing and treating pullorum salmonellosis | |
| CN103141704A (en) | Traditional Chinese medicine feed for treating swine plague | |
| CN104547770A (en) | Fermented traditional Chinese medicament for promoting growth of livestock and poultry and preparation method for fermented traditional Chinese medicament | |
| CN104523897A (en) | Enteroclysis medicine composition for treating piglet epidemic diarrhea | |
| CN104147586A (en) | Chinese herbal medicine composition and fodder for preventing and treating cold irritable enteritis of quails | |
| CN109432287B (en) | Traditional Chinese medicine oral liquid for preventing and treating excessive heat syndrome of poultry qi system and preparation method thereof | |
| CN103977058B (en) | A kind of Chinese herbal medicament oral liquid and production technology of preventing and treating chicken colibacillosis |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20140219 |