CN103977058B - A kind of Chinese herbal medicament oral liquid and production technology of preventing and treating chicken colibacillosis - Google Patents
A kind of Chinese herbal medicament oral liquid and production technology of preventing and treating chicken colibacillosis Download PDFInfo
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Abstract
The invention discloses the Chinese herbal medicament oral liquid and production technology of a kind of preventing and treating chicken colibacillosis.It is made up of following Chinese herbal medicine：Gypsum Fibrosum, Herba Senecionis Scandentiss, Herba Ecliptae, Cacumen Platycladi, Herba Euphorbiae Humifusae and Radix Codonopsis.The present invention has carried out the tests such as prescription, production technology, in-vitro antibacterial, challenge with E.coli protection respectively for chicken colibacillosis clinic syndrome, scientific composition.As a result show that said preparation can prevent and treat chicken colibacillosis well, and have no toxic side effect.
Description
Technical field
The present invention relates to a kind of medicine of preventing and treating chicken colibacillosis, specifically with system made by herbal raw material
Agent.
Background technology
Chicken colibacillosis are conditioned pathogens, and after Abwehrkraft des Koepers decline, some serotypes can cause body to be fallen ill, and go out
Existing various symptoms and pathological change, it is to have a strong impact on the pathogen that poultry develops in a healthy way with food safety.It is intensive with raising chickens
The increasingly raising of change degree, the sickness rate of colibacillosiss remain high, and sickness rate is 11～69%, and mortality rate is 3.8～
72.9%.Due to clinical medicine use it is lack of standardization, bacillus coli multiple drug resistance is increasingly serious, and some bacterial strains are even to 22 kinds
Antibiotic has drug resistance, causes poor antibacterials clinical therapeutic efficacy, drug residue height, potential source biomolecule to endanger serious etc. asking
Topic, therefore, the new and effective antibiotic preparation of research and development has become focus.
Lot of experiments has confirmed that it is efficient, long-acting, low that Chinese veterinarian's theory system instructs the lower Chinese medicine preparation developed to have
Poison, low-residual, low side effect and be not likely to produce drug resistance, eliminate R-plasmid the features such as, it has also become poultry husbandry develop in a healthy way and
The first-selected preventing and treating medicine for ensuring food safety.The Chinese medicine preparation of preventing and treating chicken colibacillosis has Radix Pulsatillae Decoction, pulvis curcumae etc. at present,
These Chinese medicine preparation have certain prevention effect to chicken colibacillosis, but still to be further improved in terms of prevention effect.
For development of new Chinese medicine antibacterial preparation, the screening study of antibacterial Chinese herbal medicineses is We conducted, on this basis according to traditional Chinese veterinary medicine
Principle-method-recipe-medicineses are required, for chicken colibacillosis clinic syndrome, scientific composition, carried out prescription, production technology respectively, have been resisted in vitro
The tests such as bacterium, challenge with E.coli protection.As a result show that said preparation can prevent and treat chicken colibacillosis well.
The content of the invention
The technical problem to be solved in the present invention is to provide a kind of Chinese medicine preparation and production technology of preventing and treating chicken colibacillosis,
The Chinese medicine preparation is evident in efficacy, has no toxic side effect.
The technical scheme is that：A kind of Chinese herbal medicament oral liquid of preventing and treating chicken colibacillosis, is characterized in that, by following
The Chinese herbal medicine of weight portion is made：Gypsum Fibrosum 28-32, Herba Senecionis Scandentiss 14-16, Herba Ecliptae 14-16, Cacumen Platycladi 18-22, Herba Euphorbiae Humifusae 9-11
With Radix Codonopsis 9-11.
Optimum ratio (weight portion)：Gypsum Fibrosum 30, Herba Senecionis Scandentiss 15, Herba Ecliptae 15, Cacumen Platycladi 20, Herba Euphorbiae Humifusae 10 and Radix Codonopsis
10。
Production technology is：
(1) medicine is weighed：Each taste Chinese herbal medicine, 4 layers of gauze list bag of Gypsum Fibrosum are weighed by above-mentioned weight portion；
(2) decoct for the first time：Gypsum Fibrosum and remaining (40～50 DEG C) immersion 0.5-1.5 hour of multi-flavor medicine difference warm water；
Gypsum Fibrosum is decocted first 25-35 minutes, then plus remaining Chinese herbal medicine boil 25-35 minutes, filter；
(3) decoct for second：Filtering residue adds (40～50 DEG C) immersion 0.5-1.5 hours of warm water, boils 40-80 minutes,
Filter, merge filtrate twice；
(4) and then concentrated, clarification, fine straining, anti-corrosion, fill, obtain the medicinal liquid of every milliliter contained 1g.
The scope of application：Fever with aversion to cold, happiness drink caused by poultry diseases caused by exogenous pathogenic factor heresy epidemic disease, discharge is just or yellow green, yellow-white, color are dilute
Just.
Using method：Prevention consumption is that 0.25～0.5ml/kg body weight, drinking-water, are used in conjunction 3～5 by 2 times/day；Treatment, 1
～1.5ml/kg body weight, drinking-water, is used in conjunction 5～7 by 2 times/day.
The property of medicine and pharmacology analysis of Chinese herbal medicine selected by of the invention is as follows：
Gypsum Fibrosum：Sulfateses mineral anhydrite race Gypsum Fibrosum.It is acrid in the mouth, sweet, it is slightly cold.Attach to the lung and stomach meridians.Tool clearing away heat-fire, it is raw
The effect of Tianjin is quenched the thirst.Cure mainly fever caused by exogenous pathogenic factors, lung-heat dyspnea with rapid and short breath, the greedy drink of gastric heat, high fever coma, manic uneasiness.Modern medicine study is demonstrate,proved
The Gypsum Fibrosum that grows directly from seeds has brings down a fever, and spasmolytic, antiinflammatory, quenches one's thirst, blood sugar lowering, calcium supplementing effect and sulfated polysaccharideization effect, enhancing immunity, promotes
Blood coagulation, function of gallbladder promoting and diuresis.Jing gastric acid effect for oral administration, a part become soluble calcium salt, enter to intestinal absorption
The concentration of calcium ion in serum, can suppress thermotaxic centre, mitigate the irritability of skeletal muscle, reduce vascular permeability, therefore have
Antipyretic, spasmolytic, antiinflammatory etc. are acted on.
Herba Senecionis Scandentiss：For feverfew Herba Senecionis Scandentiss Senecioscandens Buch, the aerial partss of-Ham..Bitter in the mouth, property
It is cold, Return liver kidney channel.The effect of tool heat-clearing and toxic substances removing, cool blood detumescence, liver heat removing and eyesight improving.For upper respiratory tract infection, tonsillitiss, eye knot
Film inflammation, dysentery, enteritis etc..Mainly containing flavoxanthin (flavoxanthin), chrysanthemum flavin (chrysanthema xanthin), Huang
Ketone, tannin, alkaloid etc..Research confirms that Herba Senecionis Scandentiss have broad-spectrum antibiotic effect, to staphylococcus aureuses, Bacillus typhi, first
Type Salmonella paratyphi, Bacillus paratyphosus B, will he, Boydii, S. sonnei have stronger inhibitory action.
Herba Ecliptae：For the herb of Compositae Shu Zhi Wu Channa intestinal Herba Echiptae.Sweet in the mouth, acid, it is cool in nature, return kidney channel, have
The effect of cooling blood for hemostasis, liver and kidney tonifying, heat-clearing and toxic substances removing.Be mainly used in treat haematemesis, epistaxis, hematuria, have blood in stool, metrorrhagia, enteritis, dysentery
Disease etc..Herb volatile oil, tannin Saponin are containing about 1.32%.Fructus seu radix camptothecae acuminatae (Fructus Camptothecae Acuminatae) blade of grass powder has significantly hemostasis effect to Canis familiaris L. half cut-off femoral artery
Really.
Cacumen Platycladi：The browse of cupressaceae plant Cacumen Platycladi Platycladus orientalis (L.) Franco.Bitterness, trembles with fear.
Enter the heart, liver, large intestine channel.The effect of with removing heat from blood, hemostasis, wind-damp dispelling, scattered toxic swelling.Be mainly used in treat haematemesis, epistaxis, hematuria,
Dysentery, hemorrhoidal hamorrhage, metrorrhagia, rheumatic arthralgia, bacillary dysentery, hypertension, cough, erysipelas, mumps are scalded.Branch and leaf bitterness containing Cacumen Platycladi
Element, absinthol, Quercetin, little ketone musk (fenchone), volatile oil.Cacumen Platycladi and contained flavonoid have relieving cough and expelling phlegm to mice
Effect；Mice tracheal smooth muscle can be made to relax, can effect of the part blocks acetylcholine to smooth muscle；This product can make laboratory animal
Mesh is closed in peace and quiet, and freely activity is reduced, and has synergism with phenobarbital；To staphylococcus aureuses, micrococcus catarrhalises, dysentery bar
Bacterium, Bacillus typhi, diphtheria corynebacterium etc. have inhibitory action.
Herba Euphorbiae Humifusae：For euphorbia plant Radix seu Caulis Parthenocissi tricuspidatae Euphorbia humifusa Willd. or Herba Euphorbiae supinae Euphorbia
Maculata L.'s is dried herb.Acrid in the mouth, it is mild-natured.Return liver, large intestine channel.The effect of tool heat-clearing and toxic substances removing, cooling blood for hemostasis.For dysentery
Disease, has loose bowels, hemoptysis, and hematuria is had blood in stool, metrorrhagia, furuncle carbuncle.Herba Euphorbiae Humifusae contains gallic acid, Galla Turcica (Galla Helepensis) formicester, quercitrin, Cortex querci dentatae
Element, inositol and tannic acid etc. gallic acid is its main antibiotic effective ingredient. the product powder local uses, for dog arteriotomy
Bleeding has obvious anastalsis；Herba Euphorbiae Humifusae can suppress the growth of diphtheria corynebacterium, also play the role of to neutralize diphtheria toxin, diphtherotoxin, can reduce
Caused by Diphtheria toxin, Cavia porcelluss are dead.The effect that the product are antibacterial, sterilize and neutralize diphtheria toxin, diphtherotoxin, with tincture preferably, decoct
Take second place, extractum is poor.
Radix Codonopsis：For the root of campanulaceae of the genus codonopsis Radix Codonopsis Codonopsis pilosula (Franch) Nannf..Taste
Sweet, micro- acid, it is mild-natured；Returns spleen, lung meridian.With invigorating the spleen and replenishing QI (kind strengthening the spleen gas), strengthening spleen and tonifying lung, anticancer, blood pressure lowering, anti-hypoxia, anti-ageing
Old the effect of, the activity of superoxide dismutase with enhancing human body immunity power, can be improved, strengthens the ability for eliminating free radical,
With Gastrointestinal motility adjustment, antiulcer, gastric acid secretion inhibiting, the effect for reducing pepsin activity.
Formula is parsed：In the present invention, Herba Euphorbiae Humifusae, Herba Ecliptae, Cacumen Platycladi, Herba Senecionis Scandentiss are respectively provided with cooling blood for hemostasis, heat-clearing
Effect, can effectively alleviate bleeding due to blood-heat, the viscera that internal organs bleeding, cellulosic ooze out, and be principal agent altogether, embody traditional medicine it
The marrow of " leaving one point of blood, possess one point of life "；Gypsum Fibrosum tool clearing away heat-fire, the work(of promoting the production of body fluid to quench thirst, can effectively release heating, happiness
The cardinal symptom of drink, is accessory drugss；Radix Codonopsis invigorating the spleen and replenishing QI, strengthening spleen and tonifying lung, with Gypsum Fibrosum compatibility, highlight QI invigorating heat-clearing, heat clearing away
Just do not hinder, while strengthening the spleen and tonifying the lung does not love heresy, recover rapidly spleen and stomach function, essence of water and grain is digested and assimilated, gas, blood, body fluid life
The source of change is sufficient, and YIN and YANG in a relative equilibrium, physical recovery are adjuvant.
The etiology and pathogenesis of chicken colibacillosis：Chicken colibacillosis belong to the disease of the damp and hot evil epidemic disease of diseases caused by exogenous pathogenic factor.When under Abwehrkraft des Koepers
During drop, escherichia coli attack appearance, fur first.Due to lung and fur phase the exterior and the interior, there is miscellaneous hair, heating, fear of cold, happiness drink, after
And nasal cavity occur mucus, the symptom such as eyes are shed tears；Pathogenic heat invade taste, taste become thoroughly decomposed food function imbalance, then there is paddy
Do not change, expect just, cause then intestinal separating clear and excreting turbid to be obstructed yellowish green loose stool occur；As pathogenic heat invades percardium, due to the heart controlling mental and emotional activitieses,
There is lethargy, coma, drowsiness, the visible pericardial effusion of cut open inspection, pericardium is muddy, thicken；As pathogenic heat attacks liver, cause liver
Dirty enlargement bleeding, even liver envelope is thickened into cellulose and oozes out film.As good and evil are mutually fought, pathogen preponderating over vital QI, bleeding due to blood-heat, spleen system
Blood functional disorder, blood oozing from the body openings or subcuta neous tissue is outside vascular, and a large amount of organ hemorrhages, such as glandular stomach, trachea, larynx, subcutaneous, intestinal etc..Therefore, select
With the medicine of cooling blood for hemostasis, the main symptom of inherent organ mucosa hemorrhage is released；From the medicine of clearing away heat-fire, heat in vivo is removed, with up to standard
This simultaneous purpose controlled.
Beneficial effects of the present invention：The present invention has carried out group respectively for chicken colibacillosis clinic syndrome, scientific composition
The tests such as side, production technology, in-vitro antibacterial, challenge with E.coli protection.As a result show that said preparation can be prevented and treated well
Chicken colibacillosis, and have no toxic side effect.Prescription of the present invention is simple, compared with more than 10 the tastes easily even prescription of more than 20 tastes, tool
Have refining formula, make a distinction between the important and the lesser one, with strong points, product quality is easily controllable, differentiate it is easy the advantages of.
Specific embodiment
Embodiment 1
Proportioning (weight portion)：Gypsum Fibrosum 30, Herba Senecionis Scandentiss 15, Herba Ecliptae 15, Cacumen Platycladi 20, Herba Euphorbiae Humifusae 10, Radix Codonopsis 10.
(1) medicine is weighed：Each taste Chinese herbal medicine, 4 layers of gauze list bag of Gypsum Fibrosum are weighed by above-mentioned weight portion；
(2) decoct for the first time：Gypsum Fibrosum adds (40～50 DEG C) of 6 times of amount warm water to soak 1 hour with remaining multi-flavor medicine respectively；It is raw
Gypsum Fibrosum is decocted first 30 minutes, then plus remaining Chinese herbal medicine boil 30 minutes, filter；
(3) decoct for second：Filtering residue adds (40～50 DEG C) of 4 times of amount warm water and soaks 1 hour, boils 1 hour, filters,
Merge filtrate twice；
(4) concentrate：Filter vacuum decompression (<1g crude drugs/1ml medicinal liquids are concentrated into 60kpa)；
(5) clarify：36 DEG C add 6%KBT-ZTC5% solution (the natural Chinese medicine clarifiers of Ke Lier research and development), and stirring is quiet
30min is put, overnight；
(6) 0.3% sodium benzoate anti-corrosion is added after fine straining；
(7) fill：When fluid temperature drops to 50 DEG C, fill while stirring, sealing.
Using method：Prevention consumption is that 0.25～0.5g/kg body weight, drinking-water, are used in conjunction 3～5 by 2 times/day；Treatment, 1～
1.5g/kg body weight, drinking-water, is used in conjunction 5～7 by 2 times/day.
2 herbal mixture In vitro Bactericidal Experiments of embodiment
1 test material
1.1 reagent agent：
Prescription one (Radix Pulsatillae Decoction)：The Radix Pulsatillae 25, Rhizoma Coptidis 25, Cortex Phellodendri 25, Cortex Fraxini 25；Decocting is boiled, 1g crude drugs/1ml medicinal liquids；
Prescription two (pulvis curcumae)：Radix Curcumae 12, Radix Et Rhizoma Rhei 12, Fructus Gardeniae 12, Radix Scutellariae 15, Cortex Phellodendri 12, Rhizoma Coptidis 25, the Fructus Chebulae 12, Radix Paeoniae Alba
12；Decocting is boiled, 1g crude drugs/1ml medicinal liquids；
Prescription three (medicine of embodiment 1)：Gypsum Fibrosum 30, Herba Senecionis Scandentiss 15, Herba Ecliptae 15, Cacumen Platycladi 20, Herba Euphorbiae Humifusae 10, party
Ginseng 10.
1.2 trial drugs are purchased from Jian Lian Chinese medicines company limited of Jinan City, and Jing differentiates to meet 2010 editions《Chinese people's republicanism
State's veterinary drug pharmacopeia》(two) requirements；Numbering is the escherichia coli of 09-29, and serotype is O24, Shandong Academy of Agricultural Sciences poultry grinds
Study carefully it is separated where strain；Beef extract, peptone, Nutrient agar, Beijing bispin microbiological culture media products factory.
1.3 key instrument equipment superclean benches, Jinan Jie Kang cleaning equipments factory product；ZD-85A digital display gas bath constant temperature shakes
Swing device, Changzhou Pu Tian instrument manufacturings company limited product.Double dish, potlery tile lid, steel pipe etc..
2 test methods
2.1 medicinal liquids prepare above-mentioned preparation 6000r/min centrifugation 3min, take 121 DEG C of autoclaving 15min of supernatant, 4 DEG C
Refrigerator store is standby.
The preparation of 2.2 bacterium solutions takes 09-29 bacterium solutions 0.1mL and is inoculated in the common NB culture medium of 5mL, into constant temperature gas bath agitator,
150r/min, 37 DEG C of overnight incubations, bacterial content are 30.0 × 1010CFU/mL.Bacterium solution low-temperature centrifugation, extracting waste precipitation, with nothing
It is standby that bacterium PBS liquid is diluted to bacterium solution volume.
2.3 drug sensitive test cup-plate methods (titer of antibodies algoscopy) measuring pipette is drawn ordinary nutrient agar culture medium and is added
In double dish, per dish 20ml, potlery tile lid is covered, it is to be solidified；Cultured test is taken, bacterium solution is added in 0.5% ratio cold
Into about 45 DEG C of ordinary nutrient agar culture medium, mix homogeneously is drawn with measuring pipette and is added in the double dish for completing bottom, stands
Double dish are rotated, is made uniformly to be laid on bottom containing bacterium culture medium, is covered potlery tile lid, it is to be solidified；Steel pipe is placed in tweezers
In the culture medium for having solidified, traditional Chinese medicine liquid is added in steel pipe with micropipettor, often 300 μ l of pipe.Proceed to 37 DEG C of constant temperature culture
Case, cultivates 24h, takes out measurement bacteriostatic diameter, averages.
3 results
1 herbal mixture in-vitro antibacterial effect (mm) of table
Remarks：* inhibition zone border is transparent clear.
As can be seen from Table 1：Three kinds of prescriptions have different degrees of bacteriostasis, maximum with the antibacterial circle diameter of prescription 3.
4 conclusions：The agent in vitro fungistatic effect of the present invention preferably, has obvious inhibitory action to escherichia coli.
Anti- coli test in 2 compound preparation body of embodiment
1 test material
1.1 trial drug prescriptions 1,3 Chinese crude drug of prescription are purchased from Jian Lian Chinese medicines company limited of Jinan City, and Jing differentiates to meet
2010 editions《People's Republic of China's veterinary drug pharmacopeia》(two) requirements；Numbering is the escherichia coli of 09-29, and serotype is O24, mountain
The detached strains of eastern Shanxi Academy of Agricultural Sciences poultry institute；19 age in days SPF cocks, Domestic Fowls Inst., Shandong Academy of Agricultural Sciences
Test site provides；Beef extract, peptone, Nutrient agar, Beijing bispin microbiological culture media products factory.
1.2 key instrument equipment superclean benches, Jinan Jie Kang cleaning equipments factory product；ZD-85A digital display gas bath constant temperature shakes
Swing device, Changzhou Pu Tian instrument manufacturings company limited product, SPF fowl raising isolators, the dynamic equipment company limited of Suzhou Feng Shi experiments
Product.
2nd, test method
The preparation of 2.1 bacterium solutions takes 09-29 bacterium solutions 0.1mL and is inoculated in the common NB culture medium of 5mL, into constant temperature gas bath agitator,
150r/min, 37 DEG C of overnight incubations, bacterial content are 30.0 × 1010CFU/mL.Bacterium solution low-temperature centrifugation, extracting waste precipitation, with nothing
It is standby that bacterium PBS liquid is diluted to bacterium solution volume.
2.2 escherichia coli LD50Determine public young from 19 age in days SPF, be divided into 0.2mL, 0.3mL, 0.4mL group, 10 per group
Chicken, intraperitoneal inoculation bacterium solution.Jing statistics 0.4mL groups 48h mortality rates are 50%, and this is escherichia coli LD50, it is defined as Inoculant according to this
Amount.
2.3 artificial infection chicken's E. coli models build chooses the public young birds of 19 age in days SPF, and aseptic intraperitoneal inoculation is centrifuged bacterium solution
0.4mL/ is only.
2.4 test group experiments divide Chinese medicine groups, attack bacterium group and blank control group, per group of 30 chickens.Chinese medicinal components do not set pre-
Anti- group, treatment group, numbering are 1-1,1-2 etc..
2.5 administrated methods attack that bacterium is forward and backward to be gavaged twice by 2mL/kg body weight point morning and afternoon, and test group shifts to an earlier date perfusion in 1 day,
Bacterium matched group and blank control group are attacked, normal saline is synchronously gavaged.
2.6 data processing itemized record attack bacterium 24h and 48h cumulative mortality.All data are with mean+SD
Represent, and count its diversity.
3 results
3.1 earliest death time preventive effects 1-1,1-2, attack bacterium control and be and attack 8h after bacterium, remaining each group is attacks bacterium
24h afterwards.
3.224h mortality rate 1-1 groups pole is significantly higher than 3-1 groups, and 1-2 groups are not notable with 3-2 group differences, and 1-2 groups, 3-1 groups are
Pole is substantially less than attacks bacterium matched group, and 3-2 groups are substantially less than and attack bacterium matched group.
3.396h survival rate 1-1 groups, 1-2 groups pole are substantially less than 5-1 groups, 5-2 groups, do not show with counteracting toxic substances matched group difference
Write；5-1 groups, 5-2 groups pole are significantly higher than and attack bacterium matched group.
Antibacterial effect table (h, %) in 2 herbal mixture body of table
4 conclusions with earliest death time, 24h mortality rates and 96h survival rates as evaluation index, imitate by the prevention and treatment of prescription 3
Fruit is significantly better than prescription 1.
3 colibacillosiss clinical effect trial of embodiment
Chicken colibacillosis natural occurrence case is selected in this test, is treated using compound preparation, to further determine which
Clinical efficacy and safety.
1 test material
1.1 medicament composing prescriptions 3 make every mL medicinal liquids 1g containing crude drug by regulation technique；Control drug, Radix Pulsatillae oral liquid, Yantai
Greenery animal health-care product company limited produces, and lot number is 20120603.
1.2 laboratory animal, 30 age in days natural occurrence AA broiler.
2 test methods
The selection of 2.1 Colibacillosis chicken groups：The spiritual depressed, feather of chicken group appearance is fluffy and disorderly, wing is sagging, it is thermophilic to close mesh
Sleep, a solitary a corner or poly- heap, arrange yellow green loose stool, there are the characteristic pathological changes such as pericarditiss, perihepatitis, airsacculitis, takes liver in cut open inspection
Disease of ZANG-organs material carries out bacteria distribution identification, is diagnosed as colibacillosiss.
2.2 test packets：Same morbidity chicken group is divided into into 3 groups, respectively 1 group of preparation, 2 groups of preparation and Radix Pulsatillae oral liquid
Treatment group, as positive drug control.
2.3 medication：Test group, every kg body weight doses are 1mL to chicken daily, divide the 2 drinking-water medication of upper and lower noon, are used in conjunction 5 days；
Radix Pulsatillae oral liquid matched group, every kg body weight doses are 1mL to chicken daily, divide the 2 drinking-water medication of upper and lower noon, are used in conjunction 5 days.
2.4 observation index
2.4.1 clinical symptoms：After administration, two group of appearance, behavioral activity, mental status, feces feelings are observed daily
Condition, morbidity and death condition etc., observation chicken group clinical symptoms improve situation.
2.4.2 body weight：Before medication and after medication, the separately sampled body weight to two groups of chickens is weighed.
2.4.3 mortality rate：Record death toll, finally calculates mortality rate daily.
3 results and analysis
3.1 clinical symptoms situations
After preparation medication group medication 2 days, chicken group's mental status start to take a turn for the better, and death toll begins to decline.After medication 5 days, extremely
Die stopping, chicken group return to normal.
After Radix Pulsatillae oral liquid matched group medication 3 days, chicken group's mental status start to take a turn for the better, and death starts to reduce.Medication 5 days
Afterwards, chicken still has fragmentary death.7 days after medication, chicken group return to normal.
Overall judge is carried out from clinical manifestation, preparation for treating group is become apparent from compared with Radix Pulsatillae oral liquid matched group effect.
3.2 body weight
Before medication, at the end of (30 age in days) and medication, when (36 age in days), the body weight of each group the results are shown in Table 3.Test group rate of body weight gain
Pole is significantly higher than Radix Pulsatillae matched group.
The body weights of 3 each group of table
Remarks：* represents that difference is extremely notable, p ＜ 0.01.
3.3 death condition
Test group chicken group's survival rate is respectively 94.6%, high compared with Radix Pulsatillae mouth matched group 2.2 percentage points, significant difference.
4 preparation for treating chicken colibacillosis clinical efficacy result of table (only, %)
Remarks：* significant difference, p ＜ 0.05. are represented
4 conclusions
By Comprehensive Comparison being carried out to two groups of chicken group's clinical symptoms, chick weight gains and mortality rates, as a result show test
Medicine dramatically increases body weight with pole compared with Radix Pulsatillae oral liquid, significantly reduces the effect of mortality rate.
Claims (3)
1. a kind of Chinese herbal medicament oral liquid of preventing and treating chicken colibacillosis, is characterized in that, be made up of the Chinese herbal medicine of following weight parts：It is raw
Gypsum Fibrosum 28-32, Herba Senecionis Scandentiss 14-16, Herba Ecliptae 14-16, Cacumen Platycladi 18-22, Herba Euphorbiae Humifusae 9-11 and Radix Codonopsis 9-11.
2. a kind of Chinese herbal medicament oral liquid of preventing and treating chicken colibacillosis as claimed in claim 1, is characterized in that, by following weight
The Chinese herbal medicine of part is made：Gypsum Fibrosum 30, Herba Senecionis Scandentiss 15, Herba Ecliptae 15, Cacumen Platycladi 20, Herba Euphorbiae Humifusae 10 and Radix Codonopsis 10.
3. the production technology of the Chinese herbal medicament oral liquid described in claim 1 or 2, is characterized in that,
(1) medicine is weighed：Each taste Chinese herbal medicine, 4 layers of gauze list bag of Gypsum Fibrosum are weighed by above-mentioned weight portion；
(2) decoct for the first time：Gypsum Fibrosum and remaining Chinese medicine medicines difference warm water immersion 0.5-1.5 hour；Gypsum Fibrosum is decocted first
25-35 minutes, then plus remaining Chinese herbal medicine boil 25-35 minutes, filter；
(3) decoct for second：Filtering residue adds warm water soaking 0.5-1.5 hours, boils 40-80 minutes, filters, and merging is filtered twice
Liquid；
(4) and then concentrated, clarification, fine straining, anti-corrosion, fill, obtain medicinal liquid.
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| CN102552656A (en) * | 2012-01-19 | 2012-07-11 | 青岛康地恩药业股份有限公司 | Chinese medicinal composition for treating chicken colibacillosis |
| CN103550314A (en) * | 2013-11-07 | 2014-02-05 | 青岛农业大学 | Veterinary medicine for treating colibacillosis and white diarrhea |
-
2014
- 2014-05-26 CN CN201410225687.9A patent/CN103977058B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102552656A (en) * | 2012-01-19 | 2012-07-11 | 青岛康地恩药业股份有限公司 | Chinese medicinal composition for treating chicken colibacillosis |
| CN103550314A (en) * | 2013-11-07 | 2014-02-05 | 青岛农业大学 | Veterinary medicine for treating colibacillosis and white diarrhea |
Non-Patent Citations (1)
| Title |
|---|
| 鸡大肠杆菌的防治;程海;《广西畜牧兽医》;19911231(第3期);第36-38页 * |
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| CN103977058A (en) | 2014-08-13 |
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