CN103830404A - Traditional Chinese medicine composition for treating Chicken colibacillosis, preparation method of traditional Chinese medicine mixture and traditional Chinese medicine mixture - Google Patents
Traditional Chinese medicine composition for treating Chicken colibacillosis, preparation method of traditional Chinese medicine mixture and traditional Chinese medicine mixture Download PDFInfo
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Abstract
The invention discloses a traditional Chinese medicine composition for treating Chicken colibacillosis. The traditional Chinese medicine composition comprises the following raw materials: a plant medicine and a mineral medicine according to the weight ratio being 300:(0.5-1.5), wherein the plant medicine comprises the following components in parts by weight: 2 to 4 parts of coptis chinensis, 4 to 6 parts of scutellaria baicalensis, 4 to 6 parts of golden cypress, 2 to 4 parts of andrographis paniculata, 3 to 5 parts of folium isatidis, 4 to 6 parts of honeysuckle, 4 to 6 parts of fructus forsythiae, 4 to 6 parts of the root of Chinese pulsatilla, 4 to 6 parts of wild chrysanthemum flower, 2 to 4 parts of rheum officinale, 3 to 5 parts of isatis root, 2 to 3 parts of radix sophorae flavescentis and 2 to 3 parts of sweet wormwood; and the mineral medicine is of realgar. The traditional Chinese medicine composition can be prepared into a mixture or granules. The traditional Chinese medicine composition is obvious in treatment effect, small in toxic and side effect, simple to prepare and convenient to take, and cannot generate medicine residue.
Description
Technical field
The present invention relates to a kind of Chinese medicine composition of chicken colibacillosis, preparation method and herb mixture of herb mixture for the treatment of.
Background technology
Chicken colibacillosis is the general name of the class disease that caused by some serotype of colon bacillus (being commonly called as escherichia coli).Chicken colibacillosis is one of common fowl disease, has the advantages that rapid onset, mortality rate are high, the course of disease is long, has caused great harm to poultry husbandry.At present, mostly adopt antibiotic and chemicals to prevent and treat colibacillosis both at home and abroad, and a large amount of uses of antibiotic and chemicals, cause colibacillary Antibiotic Resistance constantly to expand, medical treatment effect declines, residual in body of antibacterials simultaneously, has reduced the quality of meat, egg product, has affected human health and public health security.Chinese medicine has low-residual, hypotoxicity, is difficult for producing the features such as drug resistance, therefore invents a kind of Chinese medicine effective to Control of Colibacillosis of Chickens, that cure rate is high and just seems very necessary.
Summary of the invention
One of object of the present invention is to provide a kind of Chinese medicine composition for the treatment of chicken colibacillosis.
In order to realize foregoing invention object, one of the technical solution used in the present invention is that the raw material of making the effective ingredient of the Chinese medicine composition for the treatment of chicken colibacillosis comprises plant amedica and mineral drug, and the weight ratio of plant amedica and mineral drug is 300 ︰ 0.5-1.5; Composition and the weight proportion thereof of described plant amedica comprise: Rhizoma Coptidis 2-4 part, Radix Scutellariae 4-6 part, Cortex Phellodendri 4-6 part, Herba Andrographis 2-4 part, Folium Isatidis 3-5 part, Flos Lonicerae 4-6 part, Fructus Forsythiae 4-6 part, Radix Pulsatillae 4-6 part, Flos Chrysanthemi Indici 4-6 part, Radix Et Rhizoma Rhei 2-4 part, Radix Isatidis 3-5 part, Radix Sophorae Flavescentis 2-3 part, Herba Artemisiae Annuae 2-3 part; Described mineral drug is Realgar.
In order to realize foregoing invention object, two of the technical solution used in the present invention is that the raw material of making the effective ingredient of the Chinese medicine composition for the treatment of chicken colibacillosis comprises plant amedica and mineral drug, and the weight ratio of plant amedica and mineral drug is 300 ︰ 1; Composition and the weight proportion thereof of described plant amedica comprise: 3 parts of Rhizoma Coptidis, 5 parts of Radix Scutellariaes, 5 parts of Cortex Phellodendris, 3 parts of Herba Andrographis, 4 parts of Folium Isatidiss, 5 parts of Flos Loniceraes, 5 parts of Fructus Forsythiaes, 5 parts of the Radix Pulsatillaes, 5 parts of Flos Chrysanthemi Indicis, 3 parts of Radix Et Rhizoma Rhei, 4 parts of Radix Isatidis, 2.5 parts of Radix Sophorae Flavescentiss, 2.5 parts of Herba Artemisiae Annuaes; Described mineral drug is Realgar.
Two of object of the present invention is to provide a kind of preparation method of herb mixture for the treatment of chicken colibacillosis.
In order to realize foregoing invention object, preparation method provided by the invention comprises the following steps:
(1) get each taste Chinese medicine by the proportion relation of technique scheme one or two;
(2) first Rhizoma Coptidis is soaked, then Rhizoma Coptidis is sent into together with water to distillator and distilled, device to be distilled stops distillation while no longer flowing out weak yellow liquid, and the weak yellow liquid of collecting is distillate, solidliquid mixture in distillator is filtered, and the filtrate of acquisition is Rhizoma Coptidis decocting liquid;
(3) above-mentioned distillate is carried out to redistillation, make re-distilled liquid, in re-distilled liquid, add the dehydrated alcohol that is twice in its volume, after mixing, make volatile oil solution;
(4) by decocting with water after all the other Chinese medicines mixing outside Rhizoma Coptidis, produce decocting liquid, decoct altogether twice, decoct 1 hour at every turn, merge twice decoction gained decocting liquid and make mixing liquid medicine decocting liquid;
(5) above-mentioned Rhizoma Coptidis decocting liquid and mixing liquid medicine decocting liquid are mixed, then concentrating under reduced pressure makes concentrated solution; To 0.5% the sodium benzoate that adds its quality in concentrated solution, until sodium benzoate dissolve and concentrated solution cooling after, add again the 101 clarifier solution of the 2-20% of concentrated solution quality, after stirring, leaving standstill to concentrated solution has precipitate to form, then add volatile oil solution, hold over night after stirring makes supernatant after centrifugal treating, with sterilized water, supernatant is deployed into medicinal liquid and makes herb mixture, liquor strength requires the effective ingredient for containing in 1ml medicinal liquid to be equivalent to 1g crude drug.
Three of object of the present invention is to provide a kind of herb mixture for the treatment of chicken colibacillosis.
In order to realize foregoing invention object, herb mixture of the present invention is prepared by preparation method provided by the present invention.
According to the dialectical theory of Chinese veterinarian, avian colibacillosis is the interior-heat syndrome that the long-pending intestinal of damp and hot heap soil or fertilizer over and around the roots causes.Large-scale cultivation is all to adopt totally-enclosed or semiclosed mode to raise at present, and in hen house, air humidity is large, and temperature is high, particularly between brood time.Chicken is the YIN aspect of YANG, likes dry and dislikes wet.Place's wetland for a long time, susceptible exogenous damp; Hinder in exogenous damp, damp pathogen obstructing the spleen, dysfunction of the spleen in transportation is wet from interior life; And hypoactivity of spleen-YANG, water-damp not being transformed, more easily causes exogenous damp invasion and attack.The virosis of chicken, bacterial disease are commonly encountered diseases and frequently-occurring disease, the existing damp and hot card of multilist, or wet take heat as main holding concurrently, or to wet as the main heat of holding concurrently.Control suitable heat-clearing and toxic substances removing, dampness-eliminating and dysentery-stopping, monarch coordinates, and coordinates mutually, for use, can reach the effect for the treatment of both the principal and secondary aspects of a disease, strengthening vital QI to eliminate pathogenic factors.
The present invention makes according to the theory of the pathogenesis of chicken colibacillosis and tcm treatment according to syndrome differentiation, and preferred each taste Chinese medicine is suited the remedy to the case, the drug action of each taste Chinese medicine and interact as described below.
Rhizoma Coptidis, bitter in the mouth is cold in nature, effect of tool pathogenic fire purging, heat clearing away, removing toxic substances, dampness; The composition of Rhizoma Coptidis comprises multiple alkaloid, and large quantity research shows, in Rhizoma Coptidis, anti-inflammation composition is mainly the materials such as coptisine, jateorhizine, berberine and palmatine, has opposing pathogenic microorganism, enhancing human body immunity power, the effects such as gastric mucosal barrier that strengthen.
The Radix Pulsatillae, bitter in the mouth cold in nature, has heat-clearing and toxic substances removing, removing dampness and killing parasites, the effects such as eliminating pathogenic heat from blood to cure dysentery, the Radix Pulsatillae has the effect of antitumor, parasiticide, antioxidation, anti-inflammatory, immunologic function that can enhancing body.
Radix Scutellariae, bitter in the mouth cold in nature, has the effects such as eliminating fire and detoxication, cooling blood for hemostasis, heat clearing and damp drying, and main active is flavone compound, has anti-microbial infection, antitumor, anti-inflammatory, antioxidation and removes the effects such as free radical.
Cortex Phellodendri, bitter in the mouth is cold in nature, has pathogenic fire purging except effects such as steaming, heat clearing and damp drying, has the effect such as anti-inflammatory, resisting pathogenic microbes.
Radix Et Rhizoma Rhei, bitter in the mouth is cold in nature, has the effects such as eliminating fire and detoxication, dampness removing jaundice eliminating, cooling blood for hemostasis, blood circulation promoting and blood stasis dispelling, relieving constipation by purgation, and main component is the components such as emodin, chrysophanol, chrysophanic acid.Its pharmacological action mainly comprises the functions such as antitumor, resisting pathogenic microbes, anti-inflammatory, adjusting gastrointestinal function, protection cardiovascular, enhancing human body immunity.The antiinflammatory action of Radix Et Rhizoma Rhei and resisting pathogenic microbes effect are relevant to its heat-clearing toxin-expelling functions, have the function of anti-multiple virus, and as resisiting influenza virus, anti-popularity goes out fever virus, rubella virus etc.
Herba Andrographis, bitter in the mouth, cold in nature, nontoxic.There is heat-clearing and toxic substances removing, effect of removing heat from blood detumescence.Can treat acute bacillary dysentery, gastroenteritis, flu, epidemic encephalitis, tracheitis, pneumonia, pertussis, pulmonary tuberculosis, lung abscess etc.Main component is andrographolide, and pharmacological action is mainly antibacterial and antivirus action.
Folium Isatidis, bitter in the mouth is cold in nature, heat clearing away, removing toxic substances, removing heat from blood, hemostasis; Control epidemic febrile disease intenseness of heat excessive thirst, bacillary dysentery, acute gastroenteritis.
Flos Lonicerae, sweet in the mouth is cold in nature, has effect of heat-clearing and toxic substances removing.Main component is luteolin, inositol, Saponin, tannin etc.In pharmacology, mainly contain antibacterial action, in vitro various bacteria is all had to inhibitory action, generally speaking, stronger to Salmonella effect, especially typhoid fever and paratyphoid fever are had to stronger inhibition in vitro.Flos Lonicerae has some inhibitory action to Bacillus tuberculosis in vitro, and Flos Lonicerae can reduce intestinal to cholesteric absorption.
Fructus Forsythiae, bitter in the mouth is cool in nature, has heat clearing away, removing toxic substances, eliminating stagnation, the effects such as detumescence.Control warm, erysipelas, macule, carbuncle and ulcer toxic swelling.
Flos Chrysanthemi Indici, toil, cool, be mainly used to wind and heat dispersing, subduing swelling and detoxicating.Main component is for its flower is containing acacetin-7-scillabiose, yejuhua lactone, Chrysanthemin, bitter principle; Again containing volatile oil and retinoid material and vitamin B.Pharmacological action mainly contains hypotensive effect, antibiosis and antiviral functions.
Radix Isatidis, bitter in the mouth is cold in nature, heat clearing away, removing toxic substances, removing heat from blood.Radix Isatidis has fabulous antibiosis and antiviral functions, also has anti-leptospira effect and Detoxication.
Realgar, arduous, temperature, has dampness, dispels the wind, effect of parasite killing removing toxic substances.Pharmacological action mainly contains antibacterial and anti-schistosome function.
The Radix Pulsatillae, Rhizoma Coptidis, Cortex Phellodendri are harmonious and treat pyretic toxicity dysentery, and Radix Pulsatillae bitter in the mouth is cold in nature, with the bitter cold of Rhizoma Coptidis, and heat-clearing and toxic substances removing, the thick intestinal of dampness, Cortex Phellodendri is rushed down damp-heat in lower-JIAO, and Cortex Phellodendri Rhizoma Coptidis is harmonious, and wishes altogether Radix Pulsatillae heat-clearing and toxic substances removing, especially energy dampness-eliminating and dysentery-stopping, three medicines are harmonious, heat-clearing and toxic substances removing, and eliminating pathogenic heat from blood to cure dysentery, effect is strong.
Radix Scutellariae, Rhizoma Coptidis bitter cold, separate pathogenic heat in the bowels poison, and Radix Et Rhizoma Rhei bitter cold purges heat that it is long-pending to dispel, and the stasis of blood in broken intestinal, makes stagnant removing, and under blood stasis, dysentery can stop, and this is the method for the treatment of incontinent symptoms (pseudomorph) by dredging method.
Flos Lonicerae, Fructus Forsythiae heat-clearing and toxic substances removing, treatment excessive noxious heat, with Flos Lonicerae, Fructus Forsythiae is diathermanous goes out, and closes Rhizoma Coptidis bitter cold, heat-clearing and toxic substances removing, the merit expelling the heat-evil, controls intestinal decomposed rotten, particularly prompt effect.
Folium Isatidis, Radix Isatidis overweight pestilent toxicity epidemic disease, gold conjugate Flos Lonicerae, the outstanding heat clearing away poison of Radix Scutellariae, and treatment pyretic toxicity enters blood, scorchingly hot flourishing; Flos Chrysanthemi Indici gold conjugate Flos Lonicerae, the effect of clear rotten treatment excessive noxious heat is large.
Radix Sophorae Flavescentis bitter in the mouth, cold, containing multiple alkaloid, flavonoid etc.Heat clearing away, dampness, removing heat from blood, relieving heat toxin, control toxic-heat and blood stasis, discharging fresh blood stool, red and white leukorrhea inferior.
Herba Artemisiae Annuae gas perfume (or spice), mildly bitter flavor, containing amaroid, volatile oil and abrotine, vitamin etc.There is heat clearing away, removing toxic substances, control the effect such as epidemic febrile disease, dysentery.
Herba Andrographis is joined Rhizoma Coptidis treatment dysentery.
Realgar has obvious inhibitory action to common pathogenic entero becteria, and the traditional Chinese medical science is thought the clear foul gas of Realgar, because foul gas fills in intestinal, causes intestinal decomposed rotten, and Realgar is warded off dirty removing toxic substances, close Radix Et Rhizoma Rhei have loose bowels dispel long-pending, for the violent medicine of brute force, therefore consumption is few.
The present invention is take Rhizoma Coptidis as monarch drug, the Radix Pulsatillae, Radix Scutellariae, Cortex Phellodendri, Folium Isatidis, Flos Lonicerae, Realgar are ministerial drug, Herba Andrographis, Fructus Forsythiae, Flos Chrysanthemi Indici, Radix Isatidis, Radix Sophorae Flavescentis, Herba Artemisiae Annuae are adjuvant drug, Radix Et Rhizoma Rhei is for making medicine, monarch coordinates, coordinate mutually, for use, can reach the effect for the treatment of both the principal and secondary aspects of a disease, strengthening vital QI to eliminate pathogenic factors.
The invention has the advantages that: cost is low, easy to make, it is simple to use, remarkable to prevention and treatment chicken colibacillosis effect, have no drug resistance, toxic and side effects is little, and drug residue free can be produced the nuisanceless green poultry product of nutrition, high-quality, safety non-pollution.
The present invention can granulation agent or mixture, and granule is sneaked in feedstuff, and mixture joins in drinking water, and consumption is per kilogram of body weight 1-2 gram every day, is used in conjunction 3-7 days.
In the present invention, Rhizoma Coptidis should be distilled separately and be prevented loss of effective components, and adding ethanol is the dissolubility in order to increase Rhizoma Coptidis volatile oil, contains the required effective ingredient of medical treatment in volatile oil solution.Concentrating under reduced pressure needs Rotary Evaporators, and concentrated solution is contained in alembic, adopts suitable temperature, connects vacuum pump, just can under cryogenic conditions, steam like this solvent protection product thereby concentrating under reduced pressure can reduce boiling point of liquid.101 clarifiers are food stage, and by Shanghai, Wo Xun biological engineering company limited produces, and its role is to adsorbable except deproteinize, colloid and impurity.
In the present invention, the water consumption of soaking Rhizoma Coptidis take by complete Rhizoma Coptidis submergence as the lowest limit, soak time is 15-60 minute, in still-process, if the water yield continues distillation after can adding water not, until distillator stops distillation while no longer flowing out weak yellow liquid.The water consumption decocting with water after all the other Chinese medicines outside Rhizoma Coptidis are mixed take by complete medicine submergence as the lowest limit.
The specific embodiment
Embodiment 1
The raw material of making the effective ingredient of the Chinese medicine composition for the treatment of chicken colibacillosis comprises plant amedica and mineral drug, and the weight ratio of plant amedica and mineral drug is 300 ︰ 0.5; Composition and the weight proportion thereof of described plant amedica comprise: 2 parts of Rhizoma Coptidis, 4 parts of Radix Scutellariaes, 4 parts of Cortex Phellodendris, 2 parts of Herba Andrographis, 3 parts of Folium Isatidiss, 4 parts of Flos Loniceraes, 4 parts of Fructus Forsythiaes, 4 parts of the Radix Pulsatillaes, 4 parts of Flos Chrysanthemi Indicis, 2 parts of Radix Et Rhizoma Rhei, 3 parts of Radix Isatidis, 2 parts of Radix Sophorae Flavescentiss, 2 parts of Herba Artemisiae Annuaes; Described mineral drug is Realgar.
Embodiment 2
The raw material of making the effective ingredient of the Chinese medicine composition for the treatment of chicken colibacillosis comprises plant amedica and mineral drug, and the weight ratio of plant amedica and mineral drug is 300 ︰ 1; Composition and the weight proportion thereof of described plant amedica comprise: 3 parts of Rhizoma Coptidis, 5 parts of Radix Scutellariaes, 5 parts of Cortex Phellodendris, 3 parts of Herba Andrographis, 4 parts of Folium Isatidiss, 5 parts of Flos Loniceraes, 5 parts of Fructus Forsythiaes, 5 parts of the Radix Pulsatillaes, 5 parts of Flos Chrysanthemi Indicis, 3 parts of Radix Et Rhizoma Rhei, 4 parts of Radix Isatidis, 2.5 parts of Radix Sophorae Flavescentiss, 2.5 parts of Herba Artemisiae Annuaes; Described mineral drug is Realgar.
Embodiment 3
The raw material of making the effective ingredient of the Chinese medicine composition for the treatment of chicken colibacillosis comprises plant amedica and mineral drug, and the weight ratio of plant amedica and mineral drug is 300 ︰ 1.5; Composition and the weight proportion thereof of described plant amedica comprise: 4 parts of Rhizoma Coptidis, 6 parts of Radix Scutellariaes, 6 parts of Cortex Phellodendris, 4 parts of Herba Andrographis, 5 parts of Folium Isatidiss, 6 parts of Flos Loniceraes, 6 parts of Fructus Forsythiaes, 6 parts of the Radix Pulsatillaes, 6 parts of Flos Chrysanthemi Indicis, 4 parts of Radix Et Rhizoma Rhei, 5 parts of Radix Isatidis, 3 parts of Radix Sophorae Flavescentiss, 3 parts of Herba Artemisiae Annuaes; Described mineral drug is Realgar.
Embodiment 4
(1) get each taste Chinese medicine by the proportion relation of embodiment 1;
(2) first Rhizoma Coptidis is soaked, then Rhizoma Coptidis is sent into together with water to distillator and distilled, device to be distilled stops distillation while no longer flowing out weak yellow liquid, and the weak yellow liquid of collecting is distillate, solidliquid mixture in distillator is filtered, and the filtrate of acquisition is Rhizoma Coptidis decocting liquid;
(3) above-mentioned distillate is carried out to redistillation, make re-distilled liquid, in re-distilled liquid, add the dehydrated alcohol that is twice in its volume, after mixing, make volatile oil solution;
(4) by decocting with water after all the other Chinese medicines mixing outside Rhizoma Coptidis, produce decocting liquid, decoct altogether twice, decoct 1 hour at every turn, merge twice decoction gained decocting liquid and make mixing liquid medicine decocting liquid;
(5) above-mentioned Rhizoma Coptidis decocting liquid and mixing liquid medicine decocting liquid are mixed, then concentrating under reduced pressure makes concentrated solution; To 0.5% the sodium benzoate that adds its quality in concentrated solution, until sodium benzoate dissolve and concentrated solution cooling after, add again 2% 101 clarifier solution of concentrated solution quality, after stirring, leave standstill to precipitate and form, then add volatile oil solution, hold over night after stirring makes supernatant after centrifugal treating, with sterilized water, supernatant is deployed into medicinal liquid and makes herb mixture, liquor strength requires the effective ingredient for containing in 1ml medicinal liquid to be equivalent to 1g crude drug.
Embodiment 5
(1) get each taste Chinese medicine by the proportion relation of embodiment 2;
(2) this step is identical with the step (2) of embodiment 4;
(3) this step is identical with the step (3) of embodiment 4;
(4) this step is identical with the step (4) of embodiment 4;
(5) step (5) of this step and embodiment 4 is basic identical, and difference is only the consumption difference of 101 clarifier solution, and in this step, the consumption of 101 clarifier solution is 5% of concentrated solution quality.
Embodiment 6
(1) get each taste Chinese medicine by the proportion relation of embodiment 3;
(2) this step is identical with the step (2) of embodiment 4;
(3) this step is identical with the step (3) of embodiment 4;
(4) this step is identical with the step (4) of embodiment 4;
(5) step (5) of this step and embodiment 4 is basic identical, and difference is only the consumption difference of 101 clarifier solution, and in this step, the consumption of 101 clarifier solution is 10% of concentrated solution quality.
Embodiment 7
(1) get each taste Chinese medicine by the proportion relation of embodiment 3;
(2) this step is identical with the step (2) of embodiment 4;
(3) this step is identical with the step (3) of embodiment 4;
(4) this step is identical with the step (4) of embodiment 4;
(5) step (5) of this step and embodiment 4 is basic identical, and difference is only the consumption difference of 101 clarifier solution, and in this step, the consumption of 101 clarifier solution is 20% of concentrated solution quality.
Below by experiment the present invention is evaluated at the aspect such as safety, curative effect.
One, safety evaluatio
1. acute toxicological experiment preliminary experiment
Get at random 10 of kunming mouses, its fasting be can't help to water 12h, by 1.0,2.0,3.0,4.0,5.0,6.0,7.0,8.0,9.0 and the mixture prepared with embodiment 5 of the dosage of 10.0g/kgBW respectively 10 mices are carried out to 1 gavage operation, Continuous Observation 7d, laboratory animal is carried out to routine and raise, observe behavioral activity, cosmetic variation, respiratory movement, defecation situation, secretions situation and the death condition of mice.
Preliminary experiment result shows, experimental mice after medication, body weight increases, freely, appetite increases in activity, fur is smooth, cry is normal, breathe normal, without fainting from fear, sheds tears, the symptom appearance such as diarrhoea; Observing 7d, there are not the phenomena of mortality in mice, cuts open inspection and finds that major organs is without pathological change.Because detecting half lethal dose (LD50), adopt maximum tolerated dose (MTD) to identify the acute toxicology effect of medicine to mice.
2. maximum tolerated dose experiment
Get 20 of mices of SPF level balB/C (body weight 18-22g), male and female have concurrently, are divided at random 2 groups (blank group and experimental grouies), and 10 every group, water 12h is can't help in fasting; The maximal dose of experimental group take daily dosage as 60g/kgBW and the mixture of preparing with embodiment 5 as maximum administration volume using every 10g body weight administration 0.4ml carry out gavage operation to 10 mices respectively, and blank gives equivalent normal saline gavage; Continuous Observation 14d, the response situation of recording laboratory animal every day, body weight change, food ration change, death condition.
Experimental result shows, after maximal dose and maximum administration volume gavage, the symptoms such as motionless, movable minimizings that reposing appears in experiment mice short-term, bradykinesia, but without twitching, faint from fear, perpendicular hair, overturning etc. are abnormal, after 2-3h, recovery is normally gradually; , there is not dead mice in Continuous Observation 14d, and body weight, diet all increase.
Experiment finishes, and all animals is put to death, and cuts open inspection, observes the cosmetic variation of its major organs tissue, and experimental group and matched group all, without significantly pathological change, illustrate that herbal mixture does not have obvious toxic and side effects to mice.
Two, clinical trial
1. test method
210 15 Day-old Broiler Chickens are divided into 1-7 group at random, and 30 every group, the 7th group of injection physiological saline solution 1ml, in 1-6 group, every chickling is all injected same dosage O78 serotype escherichia coli suspension (concentration is 9 × 10
8cFU/mL), O78 serotype escherichia coli are purchased from China Veterinary Drugs Supervisory Inst.; O78 serotype coli strain is inoculated in common nutrient broth medium, and in 37 ℃ of shaken cultivation 16-18 hour, collecting cultured antibacterial and adjusting bacterial concentration with spectrophotometer is 9 × 10
8cFU/mL, just makes above-mentioned escherichia coli suspension.After counteracting toxic substances experiment 34h, in 1-6 group, each group all there is colibacillosis classical symptom, and the 7th group without any disease symptom; When experimental group chickling occurs after corresponding symptom, in 1-5 group every group all adopt medicine is added in drinking water and every day twice drinking-water mode treat, the 6th group and the 7th group is not carried out any Drug therapy, the above each group of duration of test all adopts conventional raising.1-3 group is all treated with herb mixture prepared by the embodiment of the present invention 5, wherein the dosage of the 1st group is containing 1ml herb mixture in every 300ml water, the dosage of the 2nd group is in every 300ml water, to contain 0.5ml herb mixture, and the dosage of the 3rd group is containing 0.25ml herb mixture in every 300ml water.The 4th group with 2.5% enrofloxacin injection treatment, and dosage is containing this injection of 1.25g in every premium on currency; The 5th group with 2% ciprofloxacin injection treatment, and dosage is containing this injection of 2g in every premium on currency.Successive administration 6 days, observes the chickling clinical symptoms change after medication, and make a record every day to clinical symptoms change, the mortality rate etc. of chickling; Dead chickling is cutd open inspection, gets diseased organ and carry out pathogenetic bacteria separation and Culture and evaluation.
2. evaluating drug effect standard
Cure: experimental session, chickling is drunk after medicine through mixed, and the symptom such as have loose bowels stops, and the situations such as its appetite, breathing, the mental status recover to be normally all considered as healing gradually.
Dead: experimental session, there is the corresponding symptom of escherichia coli dead, pathological material of disease cuts open the rear liver of inspection, air bag, heart etc. all the classical symptom of colibacillosis, and therefrom isolates escherichia coli bacterium colony, is considered as infecting dead.
3. result of the test
The 1st group of 28 healings, 2 death; The 2nd group of 27 healings, 3 death; The 3rd group of 25 healings, 5 death; The 4th group of 21 healings, 9 death; The 5th group of 22 healings, 8 death; The 6th group of 10 spontaneous recoverys, 20 death; The 7th group without there being death.
4. conclusion
From above-mentioned result of the test, be not difficult to find out, adopt three groups of herb mixture treatment chicken ehec infection disease of the present invention, its mortality rate is starkly lower than ciprofloxacin and enrofloxacin treatment matched group, and cure rate is apparently higher than ciprofloxacin and enrofloxacin treatment matched group; Therapeutic effect excellence of the present invention is described.
Test and treat contrast test by above safety evaluatio, can absolutely prove when the present invention is used for the treatment of chicken ehec infection disease, not only evident in efficacy, and have no side effect.
Claims (4)
1. treat a Chinese medicine composition for chicken colibacillosis, it is characterized in that the raw material of making said composition effective ingredient comprises plant amedica and mineral drug, the weight ratio of plant amedica and mineral drug is 300 ︰ 0.5-1.5; Composition and the weight proportion thereof of described plant amedica comprise: Rhizoma Coptidis 2-4 part, Radix Scutellariae 4-6 part, Cortex Phellodendri 4-6 part, Herba Andrographis 2-4 part, Folium Isatidis 3-5 part, Flos Lonicerae 4-6 part, Fructus Forsythiae 4-6 part, Radix Pulsatillae 4-6 part, Flos Chrysanthemi Indici 4-6 part, Radix Et Rhizoma Rhei 2-4 part, Radix Isatidis 3-5 part, Radix Sophorae Flavescentis 2-3 part, Herba Artemisiae Annuae 2-3 part; Described mineral drug is Realgar.
2. treat a Chinese medicine composition for chicken colibacillosis, it is characterized in that the raw material of making said composition effective ingredient comprises plant amedica and mineral drug, the weight ratio of plant amedica and mineral drug is 300 ︰ 1; Composition and the weight proportion thereof of described plant amedica comprise: 3 parts of Rhizoma Coptidis, 5 parts of Radix Scutellariaes, 5 parts of Cortex Phellodendris, 3 parts of Herba Andrographis, 4 parts of Folium Isatidiss, 5 parts of Flos Loniceraes, 5 parts of Fructus Forsythiaes, 5 parts of the Radix Pulsatillaes, 5 parts of Flos Chrysanthemi Indicis, 3 parts of Radix Et Rhizoma Rhei, 4 parts of Radix Isatidis, 2.5 parts of Radix Sophorae Flavescentiss, 2.5 parts of Herba Artemisiae Annuaes; Described mineral drug is Realgar.
3. treat a preparation method for the herb mixture of chicken colibacillosis, it is characterized in that comprising the following steps:
(1) get each taste Chinese medicine by the proportion relation of claim 1 or 2;
(2) first Rhizoma Coptidis is soaked, then Rhizoma Coptidis is sent into together with water to distillator and distilled, device to be distilled stops distillation while no longer flowing out weak yellow liquid, and the weak yellow liquid of collecting is distillate, solidliquid mixture in distillator is filtered, and the filtrate of acquisition is Rhizoma Coptidis decocting liquid;
(3) above-mentioned distillate is carried out to redistillation, make re-distilled liquid, in re-distilled liquid, add the dehydrated alcohol that is twice in its volume, after mixing, make volatile oil solution;
(4) by decocting with water after all the other Chinese medicines mixing outside Rhizoma Coptidis, produce decocting liquid, decoct altogether twice, decoct 1 hour at every turn, merge twice decoction gained decocting liquid and make mixing liquid medicine decocting liquid;
(5) above-mentioned Rhizoma Coptidis decocting liquid and mixing liquid medicine decocting liquid are mixed, then concentrating under reduced pressure makes concentrated solution; To 0.5% the sodium benzoate that adds its quality in concentrated solution, until sodium benzoate dissolve and concentrated solution cooling after, add again the 101 clarifier solution of the 2-20% of concentrated solution quality, after stirring, leaving standstill to concentrated solution has precipitate to form, then add volatile oil solution, hold over night after stirring makes supernatant after centrifugal treating, with sterilized water, supernatant is deployed into medicinal liquid and makes herb mixture, liquor strength requires the effective ingredient for containing in 1ml medicinal liquid to be equivalent to 1g crude drug.
4. treat a herb mixture for chicken colibacillosis, it is characterized in that described herb mixture is prepared by preparation method claimed in claim 3.
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| CN104436151A (en) * | 2014-11-13 | 2015-03-25 | 苏州市相城区盛胡特种养殖专业合作社 | Natural efficient avian antibacterial agent and preparation method thereof |
| CN104958587A (en) * | 2015-06-19 | 2015-10-07 | 安徽牧翔禽业有限公司 | Feed additive for preventing and controlling colibacillosis infection in breeding process of spotted-brown chickens |
| CN105833185A (en) * | 2016-05-09 | 2016-08-10 | 山东省农业科学院畜牧兽医研究所 | Traditional Chinese medicine composition for preventing and treating colibacillosis of domestic rabbits |
| CN106176993A (en) * | 2015-04-30 | 2016-12-07 | 普莱柯生物工程股份有限公司 | A kind of Chinese medicine composition and its preparation method and application |
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| CN106615942A (en) * | 2016-12-21 | 2017-05-10 | 广西浩昌敏再生资源利用有限公司 | Fodder for preventing and treating chicken colibacillosis and preparation method thereof |
| CN107981093A (en) * | 2017-12-15 | 2018-05-04 | 阜阳市玉琪农业科技有限公司 | A kind of feed for preventing E. coli isolated from ducks and preparation method thereof |
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