CN108783115A - A kind of immunopotentiator and the preparation method and application thereof preventing red head disease of Pseudobagrus fulvidraco - Google Patents
A kind of immunopotentiator and the preparation method and application thereof preventing red head disease of Pseudobagrus fulvidraco Download PDFInfo
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Abstract
A kind of immunopotentiator and the preparation method and application thereof preventing red head disease of Pseudobagrus fulvidraco, is related to aquaculture field.The immunopotentiator for preventing red head disease of Pseudobagrus fulvidraco includes following components by mass percentage:Chinese herb feed additive 1%-5%;Bacillus coagulans 0.1%-0.5%;Lactobacillus acidophilus 0.05%-0.25%;Bentonite 1%-5%;Yeast mannans 5%-25%;Kelp Powder 3%-15%;Glycine betaine 3%-15%;Catergen %-10%;Vitamin E2 %-10%;Surplus is carrier, and for the immunopotentiator dedicated for preventing red head disease of Pseudobagrus fulvidraco, effect is good and stablizes.The preparation method of immunopotentiator is to be uniformly mixed bacillus coagulans, lactobacillus acidophilus, yeast mannans with bentonite, then be uniformly mixed with other components, and method is simple, and preparation condition controllability is good.The application of immunopotentiator is mainly used for preparing the feed for preventing red head disease of Pseudobagrus fulvidraco.
Description
Technical field
The present invention relates to aquaculture fields, and more particularly to it is a kind of prevent red head disease of Pseudobagrus fulvidraco immunopotentiator and its
Preparation method and application.
Background technology
Pelteobagrus fulvidraco (Pelteobagrus fulvidraco), also known as yellow sharp, Huang Lading, high stickleback etc., are distributed widely in
The basins such as Yangtze River in China, the Yellow River, the Zhujiang River and Heilungkiang.Pelteobagrus fulvidraco delicious flavour, full of nutrition, few thorn have certain without squama
Tonic effect and medical value, higher price, breeding prospect generally had an optimistic view of, it has also become the important culturing economic fish in China
Class, while being the improved seeds of foreign exchange earning in the country such as Japan, South Korea, Southeast Asia also deep pro-gaze by consumer.With Huang
The rapid development of the fish intensive culture such as forehead fish, the generation of disease become the big bottleneck for restricting Pelteobagrus fulvidraco cultivation.
Pelteobagrus fulvidraco " red head disease of Pseudobagrus " is called " split head disease ", " the swollen disease of head ", is the common disease in recent years in Pelteobagrus fulvidraco breeding process
Disease is as caused by infecting " tarda ", and route of infection is via intranasal application infection olfactory cell, brain is entered back into, through brain
Film infects skull.Annual June to September is the onset peak period of red head disease of Pseudobagrus, the disease main harm Pelteobagrus fulvidraco, and parr of yellow catfish is
Main infection object is especially easy morbidity in pond high density culture Pelteobagrus fulvidraco.Early stage, sick fish without apparent clinical manifestation,
With disease, sick fish anorexia, peel off only trip, some the water surface do intermittent mad trip or long period head-up, tail
It is vertically suspended from water downward, and carrys out back rotation, fish body that What is more bends to S types, dissects visible internal organ especially liver and has
Different degrees of slight damage and lesion is mainly shown as that the quality of liver becomes fragile, nonelastic, there is slight discoloration.It falls ill the later stage,
There is " tassel flower " symptom in sick fish, overhead hyperemia, bleeding, rubescent, the crown swell upwards form useless fellow, skin of head festers,
The crown is perforated when serious, skull bone dehiscence, or even exposes brain tissue.Sick fish belly portion is expanded, and fin ray base portion, the gill cover are congested, anus
Congested, evagination.Dissect the visible intraperitoneal ascites containing largely containing blood, hepatomegaly, enteron aisle is congested, inflammation, full of light in enteric cavity
Yellow watery liquid.
The death rate is up to 90% after the onset of red head disease of Pseudobagrus, due to the infection of red head disease of Pseudobagrus be to brain external diffusion out of fish brain, once
Morbidity, external sterilizing, effects of antibiotics for oral administration are not so good, and treatment difficulty is big, can cause fish Large Scale Death, raiser is made to cover
By huge economic losses, therefore, red head disease of Pseudobagrus mainly aims at prevention.In production, often by reduce stocking density, improve water quality,
Periodically to breeding water body disinfection and the method for periodically launching the antibiotics additives such as Enrofloxacin, to reduce pathogen in water body
Quantity, enhance the disease resistance of the shoal of fish to prevent red head disease of Pseudobagrus fulvidraco.But it is added using antibiotics with long-term, a large amount of
Agent, negative effect are exposed increasingly apparently, and in the case of intensive cultivation, the premunition of Pelteobagrus fulvidraco is easily by various
The influence of disadvantage factor and reduce, so functional additive need to periodically be taken orally to improve immunity and premunition, to prevent Huang
Forehead fish red head disease of Pseudobagrus.
But it is fewer dedicated for preventing the additive product that red head disease of Pseudobagrus fulvidraco occurs at present, and effect needs to be carried
It is high.
Invention content
The purpose of the present invention is to provide a kind of immunopotentiators preventing red head disease of Pseudobagrus fulvidraco, dedicated for preventing yellow forehead
Fish red head disease of Pseudobagrus, effect is good and stablizes.
The second object of the present invention is to provide a kind of preparation method for the immunopotentiator preventing red head disease of Pseudobagrus fulvidraco, be somebody's turn to do
Method is simple, and preparation condition controllability is good.
The third object of the present invention is to provide a kind of application for the immunopotentiator preventing red head disease of Pseudobagrus fulvidraco, be used for
It prepares and prevents red head disease of Pseudobagrus fulvidraco, especially prevent the feed of parr of yellow catfish red head disease of Pseudobagrus.
The present invention solves its technical problem using following technical scheme to realize.
The present invention proposes a kind of immunopotentiator preventing red head disease of Pseudobagrus fulvidraco, by mass percentage comprising following
Component:
Further, in a preferred embodiment of the present invention, by mass percentage comprising following components:
Further, in a preferred embodiment of the present invention, Chinese herb feed additive is raised using medium-height grass liquid medicine, by weight
Number meter, Chinese herbal medicine include:3-15 parts of Chinese gall, 2-18 parts of the coptis, 5-20 parts of Herba Euphorbiae Humifusae, 5-20 parts of Asian puccoon, 5-25 parts of Radix Glycyrrhizae,
3-15 parts of Milk Thistle grass.
Further, in a preferred embodiment of the present invention, Chinese herb feed additive is raised using medium-height grass liquid medicine, by weight
Number meter, Chinese herbal medicine include:6-12 parts of Chinese gall, 5-15 parts of the coptis, 10-15 parts of Herba Euphorbiae Humifusae, 10-15 parts of Asian puccoon, Radix Glycyrrhizae 10-20
Part, 6-12 parts of Milk Thistle grass.
Further, in a preferred embodiment of the present invention, effective viable bacteria content >=1 × 10 of bacillus coagulans10CFU/
g;Effective viable bacteria content >=1.5 × 10 of lactobacillus acidophilus11CFU/g。
Further, in a preferred embodiment of the present invention, carrier in defatted rice bran, powdered rice hulls, wheat bran at least one
Kind.
A kind of preparation method of the immunopotentiator of above-mentioned prevention red head disease of Pseudobagrus fulvidraco comprising following steps:
Bacillus coagulans, lactobacillus acidophilus, yeast mannans are uniformly mixed with bentonite, then are added with Chinese herbal medicine
Agent, Kelp Powder, glycine betaine, vitamin C, vitamin E and carrier are uniformly mixed.
Further, in a preferred embodiment of the present invention, the preparation method of Chinese herb feed additive includes the following steps:By with
Than weighing the herbal powder after drying, by solid-liquid ratio 1:10-15 is heated to boiling 30-40min after soaking 20-50min,
Filtrate is collected in filtering;The dregs of a decoction are pressed into solid-liquid ratio 1:5-10 is soaked boils 15-20min again, and filtrate is collected in filtering;Merge filter
Liquid, concentrated freeze-dried powder processed.
A kind of application of the immunopotentiator of above-mentioned prevention red head disease of Pseudobagrus fulvidraco, it is yellow that immunopotentiator is used to prepare prevention
The feed of forehead fish red head disease of Pseudobagrus.
Further, in a preferred embodiment of the present invention, immunopotentiator is made an addition to by mass fraction 0.08%-0.15%
In the feed of Pelteobagrus fulvidraco.
The beneficial effect of the immunopotentiator of prevention red head disease of Pseudobagrus fulvidraco of the embodiment of the present invention and the preparation method and application thereof
Fruit is:The immunopotentiator of the prevention red head disease of Pseudobagrus fulvidraco of the embodiment of the present invention includes following components by mass percentage:In
Chinese herbal medicine additive agent 1%-5%;Bacillus coagulans 0.1%-0.5%;Lactobacillus acidophilus 0.05%-0.25%;Bentonite 1%-
5%;Yeast mannans 5%-25%;Kelp Powder 3%-15%;Glycine betaine 3%-15%;Catergen %-10%;Vitamin
E2%-10%;Surplus is carrier, and for the immunopotentiator dedicated for preventing red head disease of Pseudobagrus fulvidraco, effect is good and stablizes.The present invention
The preparation method of the immunopotentiator of the prevention red head disease of Pseudobagrus fulvidraco of embodiment is by bacillus coagulans, lactobacillus acidophilus, ferment
Female mannosan is uniformly mixed with bentonite, then with Chinese herb feed additive, Kelp Powder, glycine betaine, vitamin C, vitamin E and load
Body is uniformly mixed to get immunopotentiator, and method is simple, and preparation condition controllability is good.The prevention Pelteobagrus fulvidraco of the embodiment of the present invention
The application of the immunopotentiator of red head disease of Pseudobagrus, which is mainly used for preparing, prevents red head disease of Pseudobagrus fulvidraco, and it is red especially to prevent parr of yellow catfish
The feed of head disease.
Specific implementation mode
It in order to make the object, technical scheme and advantages of the embodiment of the invention clearer, below will be in the embodiment of the present invention
Technical solution be clearly and completely described.The person that is not specified actual conditions in embodiment, builds according to normal condition or manufacturer
The condition of view carries out.Reagents or instruments used without specified manufacturer is the conventional production that can be obtained by commercially available purchase
Product.
Below to immunopotentiator and the preparation method and application thereof of the prevention red head disease of Pseudobagrus fulvidraco of the embodiment of the present invention into
Row illustrates.
The embodiment of the present invention provides a kind of immunopotentiator preventing red head disease of Pseudobagrus fulvidraco, by mass percentage, packet
Include following components:Chinese herb feed additive 1%-5%;Bacillus coagulans 0.1%-0.5%;Lactobacillus acidophilus 0.05%-
0.25%;Bentonite 1%-5%;Yeast mannans 5%-25%;Kelp Powder 3%-15%;Glycine betaine 3%-15%;Dimension life
Plain C2%-10%;Vitamin E2 %-10%;Surplus is carrier.
In some preferred embodiments, immunopotentiator may include following components by mass percentage:Chinese herbal medicine
Additive 2%-4%;Bacillus coagulans 0.2%-0.4%;Lactobacillus acidophilus 0.1%-0.2%;Bentonite 2%-4%;Ferment
Female mannosan 10%-20%;Kelp Powder 6%-12%;Glycine betaine 6%-12%;Vitamin C 4%-8%;Vitamin E 4%-
8%;Surplus is carrier.
In some preferred embodiments, immunopotentiator may also include following components by mass percentage:In
Chinese herbal medicine additive agent 3%;Bacillus coagulans 0.3%;Lactobacillus acidophilus 0.15%;Bentonite 3%;Yeast mannans 15%;
Kelp Powder 9%;Glycine betaine 9%;Vitamin C 6%;Vitamin E 6%;Carrier 48.55%.
Wherein, Chinese herb feed additive is raised using medium-height grass liquid medicine, in some embodiments of the application, Chinese herbal medicine
Meter specifically includes in parts by weight:3-15 parts of Chinese gall, 2-18 parts of the coptis, 5-20 parts of Herba Euphorbiae Humifusae, 5-20 parts of Asian puccoon, Radix Glycyrrhizae 5-25
Part, 3-15 parts of Milk Thistle grass.Further, in some preferred embodiments of the application, Chinese herbal medicine counts packet in parts by weight
It includes:6-12 parts of Chinese gall, 5-15 parts of the coptis, 10-15 parts of Herba Euphorbiae Humifusae, 10-15 parts of Asian puccoon, 10-20 parts of Radix Glycyrrhizae, Milk Thistle grass 6-12
Part.Chinese herbal medicine selected by the present embodiment has following pharmacological action:
Chinese gall be rich in tannic acid, antibacterial and detoxicating, to gram-positive bacteria and Gram-negative bacteria have it is stronger inhibition and
Killing effect.
Coptis main component is small firewood, has lipid-loweringing, anti-oxidant, anti-apoptotic and other effects, compensates for five to a certain extent
Times single vacancy of subconstiuent, can be such that bacteriostasis enhances, to Edwardsiella tarda, Aeromonas sobria, Lu Shi not lever
Bacterium and Aeromonas hydrophila all have apparent inhibiting effect.
Herba Euphorbiae Humifusae clearing heat and promoting diuresis, cooling blood and hemostasis, removing toxicity for detumescence, herb contain flavone compound and gallic acid, antibacterial
Spectrum is wide, there is gram-positive bacteria and negative bacterium compared with high inhibition effect, has the apparent bacteriotoxin that neutralizes to act on.
Asian puccoon contains Acetylshikonin, has inhibition to Gram-negative bacteria, gram-positive bacteria through hydrolysis gained alkannin
Effect, and Asian puccoon has the function of anti-hepatocellular injury, liver protection, restores liver function, can enhance Pelteobagrus fulvidraco disease resistance indirectly, carry
High immune protective rate.
Radix Glycyrrhizae is mild-natured, sweet in flavor, is reconciled to the effect of various drugs, containing glycyrrhizic acid and liquiritin, can coordinate five
Gall nut, Asian puccoon inhibit the harmful bacteria growth in enteron aisle, and beneficial bacterium is promoted to breed, and improve intestinal health;The Radix Glycyrrhizae that Radix Glycyrrhizae contains
Glucin has suction-operated to part poisonous substance, there is the effect of removing toxic substances;In addition, Radix Glycyrrhizae can also mitigate degeneration of liver cells and downright bad journey
Degree reduces serum transaminase vigor, improves glycogen and DNA content in liver cell, promotes liver cell regeneration.
Milk Thistle grass can activate the regeneration of liver protein, restore the activity of the liver cell of damage, can also remove free radical, maintain
The permeability of liver cell film reduces damage of the toxin to liver.
Moreover, interacting between the Chinese herbal medicine that Chinese herb feed additive is selected:Chinese gall, the coptis, Herba Euphorbiae Humifusae, Asian puccoon are matched
5, cooperative compensating is acted on, very strong bacteriostasis is reached by heterogeneity.The Chinese herbal medicine compatibility of Radix Glycyrrhizae and above-mentioned antibacterial action,
Play the role of reconciliation, fungistatic effect is made to reach best;Asian puccoon, Radix Glycyrrhizae, Milk Thistle grass, which have, mitigates degeneration of liver cells and degree of necrosis,
Promote the effect of liver cell regeneration, compatible use can utmostly anti-hepatocellular injury, liver protection, recovery liver function, reduction toxin
Damage to liver, to improve the premunition of body.
Wherein, effective viable bacteria content >=1 × 10 of bacillus coagulans10CFU/g.Bacillus coagulans are amphimicrobian
Bacterium is conducive to anaerobe lactic acid bacteria and Bifidobacterium when it carries out enteron aisle breeding into that can consume free oxygen after enteron aisle
Growth, meanwhile, the consumption to free oxygen can also inhibit the harmful bacteria of aerobic existence, to adjust microorganism in enteron aisle
The balance of flora improves the immunity and premunition of body, reduces the generation of intestines problem;Bacillus coagulans are bred in enteron aisle
During will produce such as a variety of nutriments of vitamin, amino acid, short chain fatty acids to increase the speed of small bowel peristalsis, also
Meeting secreting amylase and protease improve enteron aisle digestive function, promote digestion and absorption of the body to nutriment;Condense gemma
Bacillus can also effectively activate the phagocytic activity of animal body mononuclear phagocytic cells, stimulate body secretes lysozyme, promote B lymphs thin
The proliferation of born of the same parents and the secretion of IgA, IgG, to enhance the immunity of aquatic livestock;In addition, bacillus coagulans can also generate it is super
The aliphatic acid of superoxide dismutase and short peptide chain reduces the free radical in body and neutralizes amine substance, reduces vivotoxin, subtract
The burden of few animal's liver.
Wherein, effective viable bacteria content >=1.5 × 10 of lactobacillus acidophilus11CFU/g.Lactobacillus acidophilus has good tolerance
Property, it can be colonized in animal intestinal tract, improve intestinal acidity environment, promote absorption of nutrient ingredients, reduce the toxicants such as ammonia
Accumulation, balance intestinal flora promote growth of animal, and stimulation Mucosal Immunity is expressed, to improve immunity, premunition.
Wherein, yeast mannans are the antigen active substances extracted from Yeast Cultivation cell wall, optionally by
The probiotics such as lactic acid bacteria, Bifidobacterium utilize, and promote probiotics breeding, while still excellent immunoactivator, free radical are clear
Except agent, have effects that, antibacterial anti-inflammatory, antiviral, protection enteron aisle.
Wherein, the laminarin that Kelp Powder contains is a kind of immunomodulator, can directly be carried out to macrophage, T cell
Immunological regulation, to improve immunity;Intestinal pH can be reduced simultaneously, inhibited the breeding of the harmful bacterias such as enteron aisle Escherichia coli, maintained intestines
Road health.
Wherein, glycine betaine has the delicate flavour of sweet taste and fishes and shrimps sensitivity and the chemistry knot of suitable Pelteobagrus fulvidraco osmoreceptor
Structure is ideal phagostimulant, digestive juice secretion is made to reinforce, and various digestive enzyme activities improve, and decomposes digestion feed and accelerates, stomach and intestine are compacted
It is dynamic to reinforce.Meanwhile helping to improve the situation that food ration declines under disease or stress situation, to maintain high nutrition to take in,
Certain state of an illness or stress reaction can be mitigated.
Wherein, the ability of body synthesis immune antiboidy can be improved in vitamin E, peomotes developing immune system, simultaneously
With very strong oxidation resistance.Animal illness or by stress when, the demand to VC can be improved, but Pelteobagrus fulvidraco cannot close
At enough VC, therefore VC is added in the present embodiment.Moreover, vitamin C can be cooperateed with vitamin E it is anti-oxidant, protect feed in
Other nutritional ingredients, reduce damage of the lipid peroxide to liver, there is liver protection function, improve animal resisting stress energy
Power, while vitamin C also has the function of restoring vitamin E.
Wherein, carrier is selected from least one of defatted rice bran, powdered rice hulls, wheat bran.
Component safety selected by the immunopotentiator of the embodiment of the present invention, without hazard residue, for production green feed and aquatic products
Product create condition;Formula is reasonable, has cooperative compensating effect between each component, and compatible use, which reaches, prevents red head disease of Pseudobagrus fulvidraco
Effect, effect is good and stablizes.Wherein, Chinese herb feed additive is made of different Chinese herbal medicine compatibilities, is not only reached very strong antibacterial
Effect, can also utmostly anti-hepatocellular injury, liver protection, restore liver function, reduce damage of the toxin to liver, to improve machine
The premunition of body;Because the cause of disease of red head disease of Pseudobagrus fulvidraco is mainly caused by enteric infection, the facultative anaerobic bacteria of bacillus coagulans is special
Property make its small enteral consume free oxygen, reduce enteral oxygen concentration, promote the breeding of anaerobic bacteria lactobacillus acidophilus, the two is common
The balance of intestinal microflora is adjusted, intestinal immunological barrier effect is increased;Meanwhile yeast mannans can be condensed gemma bar
Bacterium, lactobacillus acidophilus Selection utilization, can not only play the activity of probiotics, but also make probiotics quickly and mass propagation, make prebiotic work
With significantly persistently;Probiotics makes its change using the enzyme system degradation Chinese medicine of itself secretion and the protein in Kelp Powder and polysaccharide
At the amino acid, small peptide and low molecule carbohydrate for being easy to digest and assimilate, while generating the various bioactivity with anti-oxidation efficacy
Substance has acted on synergy to each ingredient of Chinese medicine.In addition, Radix Glycyrrhizae, Milk Thistle grass, glycine betaine, VE, VC can cooperate with, to improve body anti-oxidant
Ability and anti-stress ability;The palatability of entire additive can be changed in glycine betaine.Therefore, the above component collaboration enhances body
Immunity and premunition reduce damage of the toxin to liver, reach hepatoprotective effect, while it is long numerous to promote intestinal beneficial bacterium to be born
It grows, inhibits harmful bacteria proliferation in enteron aisle, to achieve the effect that prevent red head disease of Pseudobagrus fulvidraco.
In addition, the embodiment of the present invention also provides a kind of preparation side of the immunopotentiator of above-mentioned prevention red head disease of Pseudobagrus fulvidraco
Method may include following steps:
Bacillus coagulans, lactobacillus acidophilus, yeast mannans are uniformly mixed with bentonite, then are added with Chinese herbal medicine
Agent, Kelp Powder, glycine betaine, vitamin C, vitamin E and carrier are uniformly mixed to get immunopotentiator.
The preparation method of the immunopotentiator of the embodiment of the present invention is first sweet by bacillus coagulans, lactobacillus acidophilus, yeast
Dew glycan is mixed with bentonite, and bentonite can play probiotics itself protection and adhewsive action, improves probiotics survival rate, is coagulated
Knot bacillus, lactobacillus acidophilus mix with yeast manna oligosacchride, probiotics can be made preferably to play a role.It is again that content is few
Component be uniformly mixed step by step with carrier, each component can be made to be evenly distributed.The preparation side of the immunopotentiator of the embodiment of the present invention
Method is simple, and condition controllability is good, and stability is good, has good application prospect.
Wherein, Chinese herb feed additive is raised using medium-height grass liquid medicine.In some embodiments of the application, Chinese herbal medicine
The preparation method of additive may include following steps:Chinese herbal medicine is taken respectively:Chinese gall, the coptis, Herba Euphorbiae Humifusae, Asian puccoon, Radix Glycyrrhizae, Milk Thistle
Grass, drying crush, cross 200 mesh sieve;Then the herbal powder after drying is weighed according to the ratio, and specifically meter can claim in parts by weight
3-15 parts of Chinese gall, 2-18 parts of the coptis, 5-20 parts of Herba Euphorbiae Humifusae, 5-20 parts of Asian puccoon, 5-25 parts of Radix Glycyrrhizae, 3-15 parts of Milk Thistle grass are taken, is pressed
Solid-liquid ratio 1:10-15, which soaks to be heated to changing after boiling after 20-50min, is cooked by slow fire 30-40min, filters, collects filtrate;
The dregs of a decoction are pressed into solid-liquid ratio 1:5-10 is soaked boil again after change and be cooked by slow fire 15-20min, filter, collect filtrate;Merge filter
Liquid, concentrated freeze-dried powder processed.
In addition, the embodiment of the present invention also provides a kind of application of the immunopotentiator of above-mentioned prevention red head disease of Pseudobagrus fulvidraco,
Immunopotentiator especially prevents the feed of parr of yellow catfish red head disease of Pseudobagrus mainly for the preparation of red head disease of Pseudobagrus fulvidraco is prevented.In reality
In the application of border, specifically a kind of application mode can be:Immunopotentiator makes an addition to yellow forehead by mass fraction 0.08%-0.15%
In the feed of fish.Immunopotentiator can enhance the immunity and premunition of Pelteobagrus fulvidraco, improve function of intestinal canal, protect liver, reach
Prevent the effect of red head disease of Pseudobagrus fulvidraco, while the growth performance of Pelteobagrus fulvidraco also can be improved, improve body colour, improves economic value.
The feature and performance of the present invention are described in further detail with reference to embodiments.
Embodiment 1
The present embodiment provides a kind of immunopotentiator, it is made using following preparation method:
First prepare Chinese herb feed additive:Chinese gall, the coptis, Herba Euphorbiae Humifusae, Asian puccoon, Radix Glycyrrhizae, Milk Thistle grass, drying, powder are taken respectively
The broken, sieve of 200 mesh excessively;Then Chinese gall 1kg, coptis 1kg, Herba Euphorbiae Humifusae 1kg, Asian puccoon 1kg, Radix Glycyrrhizae 1.5kg, milk after sieving are weighed
Ji grass 1kg, by solid-liquid ratio 1:12 soak to be heated to changing after boiling after 30min and are cooked by slow fire 40min, filter, collect filtrate;
The dregs of a decoction are pressed into solid-liquid ratio 1:8 soak boil again after change and be cooked by slow fire 18min, filter, collect filtrate;Merging filtrate, concentration
Freeze-drying powder processed.
Prepare raw material:Chinese herb feed additive 1g, bacillus coagulans 0.1g, lactobacillus acidophilus 0.05g, bentonite 1g, ferment
Female mannosan 5g, Kelp Powder 3g, glycine betaine 3g, VC2g, VE2g, defatted rice bran 82.85g, total 100g.
Bacillus coagulans, lactobacillus acidophilus, yeast mannans are uniformly mixed with bentonite, then are added with Chinese herbal medicine
Agent, Kelp Powder, glycine betaine, VC, VE and defatted rice bran are uniformly mixed to get immunopotentiator.
Embodiment 2
The present embodiment provides a kind of immunopotentiator, the preparation methods of the preparation method and embodiment 1 of the immunopotentiator
It is roughly the same, the difference is that:
Prepare raw material:Chinese herb feed additive 2g, bacillus coagulans 0.2g, lactobacillus acidophilus 0.1g, bentonite 2g, yeast
Mannosan 10g, Kelp Powder 6g, glycine betaine 6g, VC4g, VE4g, defatted rice bran 65.7g, total 100g.
Embodiment 3
The present embodiment provides a kind of immunopotentiator, the preparation methods of the preparation method and embodiment 1 of the immunopotentiator
It is roughly the same, the difference is that:
Prepare raw material:Chinese herb feed additive 3g, bacillus coagulans 0.3g, lactobacillus acidophilus 0.15g, bentonite 3g, ferment
Female mannosan 15g, Kelp Powder 9g, glycine betaine 9g, VC6g, VE6g, defatted rice bran 48.55g, total 100g.
Embodiment 4
The present embodiment provides a kind of immunopotentiator, the preparation methods of the preparation method and embodiment 1 of the immunopotentiator
It is roughly the same, the difference is that:
Prepare raw material:Chinese herb feed additive 4g, bacillus coagulans 0.4g, lactobacillus acidophilus 0.2g, bentonite 4g, yeast
Mannosan 20g, Kelp Powder 12g, glycine betaine 12g, VC8g, VE8g, defatted rice bran 31.4g, total 100g.
Embodiment 5
The present embodiment provides a kind of immunopotentiator, the preparation methods of the preparation method and embodiment 1 of the immunopotentiator
It is roughly the same, the difference is that:
Prepare raw material:Chinese herb feed additive 5g, bacillus coagulans 0.5g, lactobacillus acidophilus 0.25g, bentonite 5g, ferment
Female mannosan 25g, Kelp Powder 15g, glycine betaine 15g, VC10g, VE10g, defatted rice bran 14.25g, total 100g.
Embodiment 6
The present embodiment provides a kind of immunopotentiator, the preparation methods of the preparation method and embodiment 1 of the immunopotentiator
It is roughly the same, the difference is that:
First prepare Chinese herb feed additive:Chinese gall, the coptis, Herba Euphorbiae Humifusae, Asian puccoon, Radix Glycyrrhizae, Milk Thistle grass, drying, powder are taken respectively
The broken, sieve of 200 mesh excessively;Then the Chinese gall 0.5kg after sieving, coptis 0.5kg, Herba Euphorbiae Humifusae 1.5kg, Asian puccoon 1.5kg, Radix Glycyrrhizae are weighed
1kg, Milk Thistle grass 1.5kg, by solid-liquid ratio 1:10 soak to be heated to changing after boiling after 50min and are cooked by slow fire 35min, filter,
Collect filtrate;The dregs of a decoction are pressed into solid-liquid ratio 1:5 soak boil again after change and be cooked by slow fire 20min, filter, collect filtrate;Merge
Filtrate, concentrated freeze-dried powder processed.
Embodiment 7
The present embodiment provides a kind of immunopotentiator, the preparation methods of the preparation method and embodiment 2 of the immunopotentiator
It is roughly the same, the difference is that:
First prepare Chinese herb feed additive:Chinese gall, the coptis, Herba Euphorbiae Humifusae, Asian puccoon, Radix Glycyrrhizae, Milk Thistle grass, drying, powder are taken respectively
The broken, sieve of 200 mesh excessively;Then the Chinese gall 1.5kg after sieving, coptis 1.5kg, Herba Euphorbiae Humifusae 1.5kg, Asian puccoon 0.5kg, Radix Glycyrrhizae are weighed
1kg, Milk Thistle grass 1.5kg, by solid-liquid ratio 1:15 soak to be heated to changing after boiling after 20min and are cooked by slow fire 30min, filter, receive
Collect filtrate;The dregs of a decoction are pressed into solid-liquid ratio 1:10 soak boil again after change and be cooked by slow fire 15min, filter, collect filtrate;Merge filter
Liquid, concentrated freeze-dried powder processed.
Embodiment 8
The present embodiment provides a kind of immunopotentiator, the preparation methods of the preparation method and embodiment 3 of the immunopotentiator
It is roughly the same, the difference is that:
First prepare Chinese herb feed additive:Chinese gall, the coptis, Herba Euphorbiae Humifusae, Asian puccoon, Radix Glycyrrhizae, Milk Thistle grass, drying, powder are taken respectively
The broken, sieve of 200 mesh excessively;Then the Chinese gall 0.5kg after sieving, coptis 0.5kg, Herba Euphorbiae Humifusae 1.5kg, Asian puccoon 1.5kg, Radix Glycyrrhizae are weighed
1kg, Milk Thistle grass 1.5kg, by solid-liquid ratio 1:10 soak to be heated to changing after boiling after 50min and are cooked by slow fire 35min, filter,
Collect filtrate;The dregs of a decoction are pressed into solid-liquid ratio 1:5 soak boil again after change and be cooked by slow fire 20min, filter, collect filtrate;Merge
Filtrate, concentrated freeze-dried powder processed.
Embodiment 9
The present embodiment provides a kind of immunopotentiator, the preparation methods of the preparation method and embodiment 4 of the immunopotentiator
It is roughly the same, the difference is that:
First prepare Chinese herb feed additive:Chinese gall, the coptis, Herba Euphorbiae Humifusae, Asian puccoon, Radix Glycyrrhizae, Milk Thistle grass, drying, powder are taken respectively
The broken, sieve of 200 mesh excessively;Then the Chinese gall 1.5kg after sieving, coptis 1.5kg, Herba Euphorbiae Humifusae 1.5kg, Asian puccoon 0.5kg, Radix Glycyrrhizae are weighed
1kg, Milk Thistle grass 1.5kg, by solid-liquid ratio 1:15 soak to be heated to changing after boiling after 20min and are cooked by slow fire 30min, filter, receive
Collect filtrate;The dregs of a decoction are pressed into solid-liquid ratio 1:10 soak boil again after change and be cooked by slow fire 15min, filter, collect filtrate;Merge filter
Liquid, concentrated freeze-dried powder processed.
Comparative example 1
Comparative example provides a kind of immunopotentiator, and the preparation method of the preparation method and embodiment 3 of the immunopotentiator is big
Cause it is identical, the difference is that:
Prepare raw material:Bacillus coagulans 0.3g, lactobacillus acidophilus 0.15g, bentonite 3g, yeast mannans 15g, sea
Band powder 9g, glycine betaine 9g, VC6g, VE6g, defatted rice bran 51.55g, total 100g.
Comparative example 2
Comparative example provides a kind of immunopotentiator, and the preparation method of the preparation method and embodiment 3 of the immunopotentiator is big
Cause it is identical, the difference is that:
Prepare raw material:Chinese herb feed additive 3g, bacillus coagulans 0.3g, bentonite 3g, yeast mannans 15g, sea
Band powder 9g, glycine betaine 9g, VC6g, defatted rice bran 54.7g, total 100g.
Comparative example 3
Comparative example provides a kind of immunopotentiator, and following preparation method is used to be made:
Barberry extract 40kg, China tree leaf extract 10kg, sodium chloride 25kg, citric acid 6kg, sulfamic acid 10kg are taken,
Cocoamidopropyl betaine 9kg is uniformly mixed and preparation can be completed.The preparation method of its Berberine extract is:Take barberry
Fruit is dried under the conditions of 80 DEG C, after the barberry fruit after drying is crushed to obtain the final product;The preparation method of China tree leaf extract is:It takes
Fresh Folium Meliae 10kg, is put into after smashing to pieces in 20L containers, is stirred evenly after filling clear water, and shady place is fermented 7 days, then will be residual
Slag is filtered with 20 mesh screens, remaining liq drying under the conditions of 50 DEG C.
The effect of the immunopotentiator of the present embodiment is detected below by way of feeding experiment.
Test example 1
It is designed using single-factor, after a week by fish domestication, selects physically fit, neat specification (counterpoise is 1 ± 0.03g)
960 tail of parr of yellow catfish is randomly divided into 6 processing groups (each processing group is divided into 4 repeating groups, 40 tail of each repeating groups), point
It Wei not control group and 5 test groups (test group 1, test group 2, test group 3, test group 4 and test group 5).The examination of 6 processing groups
Fish (cylindrical, installation of water 50L) cultivation in 24 aquariums is tested, feeding 3 times daily, (time is respectively 7:30,12:30,17:
30) it, is satiated with food and feeds, feed lasting 30min every time, ensure that feed is ingested by fish as far as possible, reduce feed waste, record is fed
Amount, cultivation phase are 8 weeks.
The feed formula of 6 processing groups is as shown in Table 1 and Table 2, and it is 2mm or so that feed is machined to grain size with small particle
Particle, room temperature air-dries and is placed on 4 DEG C of refrigerators and saves backup.Wherein, the feed of control group is basic daily ration, 5 test groups
(additive amount of immunopotentiator is to raise to the basal diet of the respectively corresponding immunopotentiator added with embodiment 1-5 of feed
Expect the 0.1% of total amount).In addition, timing (7 daily:30 and 17:30) water temperature, dissolved oxygen and the pH of water body in aquarium are measured, and fixed
Phase measures water quality.During cultivation, take natural lighting condition (round the clock than for 10h:14h, water temperature control are kept in (27 ± 3) DEG C
Water pH value is 7.5-8.0, and dissolved oxygen content is more than 5mgL-1, ammonia-nitrogen content is less than 0.4mgL-1, content of nitrite is small
In 0.06mgL-1。
1 basal diet of table forms
Daily ration forms | Control group/% | Test group (5 groups)/% |
Fish meal | 33 | 33 |
Casein | 27.5 | 27.5 |
Dextrin | 25.17 | 25.17 |
Soya-bean oil | 3.04 | 3.04 |
Fish oil | 3.04 | 3.04 |
Microcrystalline cellulose | 5.45 | 5.35 |
Di(2-ethylhexyl)phosphate fine particle calcium carbonate | 1.8 | 1.8 |
Compound premix | 1 | 1 |
Immunopotentiator | 0 | 0.1 (being respectively 5 groups of immunopotentiators) |
It is total | 100 | 100 |
Note:Premix provides following minerals (gkg for every kilogram of feed-1Of diet) and vitamin (IU or mg
kg-1of diet):CuSO4·5H2O 0.02g;FeSO4·7H2O 0.25g;ZnSO4·7H2O 0.22g;MnSO4·4H2O
0.07g;Na2SeO30.0004g;KI 0.00026g;CoCl2·6H2O 0.001g;Vitamin A 9000IU;Vitamin D
2000IU;Vitamin E 45mg;Vitamin K32.2mg;Vitamin B13.2mg;Vitamin B210.9mg;Niacin 20mg;Dimension
Raw element B65mg;Vitamin B120.016mg;Vitamin C 50mg;Pantothenic acid 10mg;Folic acid 1.65mg;Choline 600mg;Inositol
10mg;Biotin 0.012mg.
The immunopotentiator of 2 different disposal group of table forms
Culture experiment terminates, and fasting is for 24 hours.It counts the mantissa of fish in each aquarium and claims gross weight.Then each repeating groups
It selects the undamaged healthy test fish of 30 urosome tables and is immersed in a concentration of 3.0 × 1071h in the bacterial suspension of cfu/ml, then puts
It returns in aquarium and continues to cultivate, record death condition, pick dead fish, be observed continuously 5 days.
Wherein, bacterial suspension preparation method is:Disease symptom typically sick fish is taken, sick fish body is rinsed with sterile saline
Table after aseptically dissecting, is sampled with oese from the brain tissue at liver, the rubescent position of kidney and head, ordinary nutritional
Agar medium plate streaking, for 24 hours, the consistent dominant colony of picking form is inoculated with TSB Liquid Cultures for 28 DEG C of constant-temperature table cultures
In base, for 24 hours, -20 DEG C save backup for 28 DEG C of constant-temperature table cultures.Take -20 DEG C preservation experiment inoculation TSA tablets, 28
DEG C culture 48h, washes lower culture using sterilizing and is diluted to a concentration of 3.0 × 107The bacteria suspension of cfu/ml.
After challenge test, count the mantissa of fish in aquarium, by whether there is or not the fishes of disease symptom with a concentration of
100mg·L-1MS-222 (Sigma, the U.S.) anesthesia, be placed in tail vein blood on ice pan, blood collection is placed on pre-fabricated
Anticoagulant tube in, 3000rmin-110min (4 DEG C) is centrifuged, supernatant is collected, sample preserves in -20 DEG C of refrigerator freezings, is to be measured.
The calculation formula of test fish growth performance index is as follows:
Rate of body weight gain (%)=(the last first counterpoise of counterpoise -)/first counterpoise × 100%;
Feed coefficient (FCR)=daily ration, feeding quantity/(the last first counterpoise of counterpoise -);
Total mantissa × 100% when total mantissa/on-test when survival rate (%)=off-test;
There are the total mantissa of red head disease of Pseudobagrus classical symptom/Gong Duzong mantissa in incidence %=.
Table 3 attacks the comparison of different disposal group rate of body weight gain, feed coefficient before poison
Project | Control group | Test group 1 | Test group 2 | Test group 3 | Test group 4 | Test group 5 |
Survival rate % | 90%b | 92.5%a | 95%a | 97.5%a | 95%a | 95%a |
Rate of body weight gain % | 115.09b | 118.05a | 120.09a | 120.46a | 119.20a | 118.45a |
Feed coefficient | 3.10a | 2.69bc | 2.38c | 2.32c | 2.42c | 2.86b |
Note:Colleague's subscript does not have same letter to indicate significant difference (P<0.05), similarly hereinafter.
As shown in Table 3, it attacks (the being added with immunopotentiator) survival rate of test group before poison and rate of body weight gain is significantly higher than control group
(P<0.05);Test group feed coefficient is substantially less than control group (P<0.05), show the Immune-enhancing effect of the addition embodiment of the present invention
The growth and survival of parr of yellow catfish can be improved in agent, saves feed.
Table 4 attacks the comparison of different disposal group death condition and survival rate after poison
Project | Control group | Test group 1 | Test group 2 | Test group 3 | Test group 4 | Test group 5 |
There is abnormal time h | 24b | 72a | 82a | 96a | 96a | 72a |
There is death time h | 70b | 89a | 96a | - | - | 91a |
Incidence % in 5 days | 40a | 6.67b | 6.67b | 3.33b | 3.33b | 6.67b |
Survival rate % after 5 days | 66.7b | 93.3a | 96.7a | 100a | 100a | 93.3a |
As shown in Table 4, after attacking poison, compared with the control group, incidence substantially reduces test group, and survival rate significantly rises, table
The immunopotentiator of the bright addition embodiment of the present invention can substantially reduce the incidence (P of parr of yellow catfish red head disease of Pseudobagrus<0.05) it, improves
Its survival rate (P<0.05), also there is certain therapeutic effect.
Table 5 is attacked different disposal group immune indexes and hepatic injury after poison and is compared
As shown in Table 5, after attacking poison, glutamic-oxalacetic transaminease, the gpt activity of test group significantly drop compared with the control group
Low (P<0.05), show that the immunopotentiator for adding the embodiment of the present invention can reduce parr of yellow catfish hepar damnification;Test group
Complement ACH50 contents and lysozyme activity significantly increase (P compared with the control group<0.05), show to add the embodiment of the present invention
Parr of yellow catfish immunity of organisms can be improved in immunopotentiator.
Test example 2
It is tested according to the method for test example 1, grouping is respectively that control group and 4 test groups, control group obtain feed and be
Basal diet, the basal diet that the feed of 4 test groups corresponds to be added with respectively the immunopotentiator of embodiment 6-9 are (immune
The additive amount of reinforcing agent is the 0.1% of feed total amount).
Attack different disposal group rate of body weight gain before poison, the comparison result of feed coefficient is shown:Test group is (added with immune before attacking poison
Reinforcing agent) survival rate and rate of body weight gain be significantly higher than control group;Test group feed coefficient is substantially less than control group;
The comparison result of different disposal group death condition and survival rate is shown after attacking poison:After attacking poison, test group and control group
It compares, incidence substantially reduces, and survival rate significantly rises;
Different disposal group immune indexes and hepatic injury comparison result after poison is attacked to show:After attacking poison, the millet straw of test group turns ammonia
Enzyme, gpt activity significantly reduce compared with the control group, the complement ACH50 contents of test group and lysozyme activity with compare
Group, which is compared, significantly to be increased.
Test example 3
It is tested according to the method for test example 1, grouping is respectively 3 control groups (control group 1, control group 2, control groups
3) and 2 test groups (test group 1, test group 2), the feed of 3 control groups correspond to being immunized added with comparative example 1-3 respectively
The basal diet of reinforcing agent, the feed of 2 test groups are respectively the basis of the immunopotentiator added with embodiment 1, embodiment 6
Daily ration, the additive amount of immunopotentiator are the 0.1% of feed total amount.
Attack different disposal group rate of body weight gain before poison, the comparison result of feed coefficient is shown:Attack test group survival rate and increasing before poison
Rate is significantly higher than control group 3 again, is higher than control group 1-2, and in test group, and the survival rate and rate of body weight gain of test group 1 are higher than experiment
Group 2;
The comparison result of different disposal group death condition and survival rate is shown after attacking poison:After attacking poison, the incidence of test group
Substantially less than control group 3 is less than control group 1-2, and in test group, and the incidence of test group 1 is less than test group 2;
Different disposal group immune indexes and hepatic injury comparison result after poison is attacked to show:After attacking poison, the millet straw of test group turns ammonia
Enzyme, gpt activity significantly reduce compared with the control group, the complement ACH50 contents of test group and lysozyme activity with compare
Group, which is compared, significantly to be increased.
By above-mentioned experiment it is found that the immunopotentiator of the embodiment of the present invention makes an addition in feed and applies, Pelteobagrus fulvidraco can be prevented
Juvenile fish red head disease of Pseudobagrus enhances the immunity and premunition of Pelteobagrus fulvidraco, improves function of intestinal canal, protects liver, may additionally facilitate yellow forehead in addition
Fish juvenile growth saves feed.
In conclusion the immunopotentiator of the prevention red head disease of Pseudobagrus fulvidraco of the embodiment of the present invention is dedicated for prevention Pelteobagrus fulvidraco
Red head disease of Pseudobagrus, effect is good and stablizes;The preparation method of the immunopotentiator of the prevention red head disease of Pseudobagrus fulvidraco of the embodiment of the present invention is simple,
Preparation condition controllability is good;The application of the immunopotentiator of the prevention red head disease of Pseudobagrus fulvidraco of the embodiment of the present invention is used to prepare prevention
Red head disease of Pseudobagrus fulvidraco especially prevents the feed of parr of yellow catfish red head disease of Pseudobagrus.
Embodiments described above is a part of the embodiment of the present invention, instead of all the embodiments.The reality of the present invention
The detailed description for applying example is not intended to limit the range of claimed invention, but is merely representative of the selected implementation of the present invention
Example.Based on the embodiments of the present invention, those of ordinary skill in the art are obtained without creative efforts
Every other embodiment, shall fall within the protection scope of the present invention.
Claims (10)
1. a kind of immunopotentiator preventing red head disease of Pseudobagrus fulvidraco, which is characterized in that by mass percentage comprising with the following group
Point:
2. the immunopotentiator according to claim 1 for preventing red head disease of Pseudobagrus fulvidraco, which is characterized in that by weight percent
Meter comprising following components:
3. the immunopotentiator according to claim 1 for preventing red head disease of Pseudobagrus fulvidraco, which is characterized in that the Chinese herbal medicine adds
Adding agent is raised using medium-height grass liquid medicine, is counted in parts by weight, and the Chinese herbal medicine includes:3-15 parts of Chinese gall, 2-18 parts of the coptis,
5-20 parts of Herba Euphorbiae Humifusae, 5-20 parts of Asian puccoon, 5-25 parts of Radix Glycyrrhizae, 3-15 parts of Milk Thistle grass.
4. the immunopotentiator according to claim 3 for preventing red head disease of Pseudobagrus fulvidraco, which is characterized in that the Chinese herbal medicine adds
Adding agent is raised using medium-height grass liquid medicine, is counted in parts by weight, and the Chinese herbal medicine includes:6-12 parts of Chinese gall, 5-15 parts of the coptis,
10-15 parts of Herba Euphorbiae Humifusae, 10-15 parts of Asian puccoon, 10-20 parts of Radix Glycyrrhizae, 6-12 parts of Milk Thistle grass.
5. the immunopotentiator according to claim 1 for preventing red head disease of Pseudobagrus fulvidraco, which is characterized in that bacillus coagulans
Effective viable bacteria content >=1 × 1010CFU/g;Effective viable bacteria content >=1.5 × 10 of lactobacillus acidophilus11CFU/g。
6. the immunopotentiator according to claim 1 for preventing red head disease of Pseudobagrus fulvidraco, which is characterized in that the carrier is selected from
At least one of defatted rice bran, powdered rice hulls, wheat bran.
7. a kind of preparation method of the immunopotentiator such as according to any one of claims 1 to 6 preventing red head disease of Pseudobagrus fulvidraco,
It is characterized in that, it includes the following steps:
Bacillus coagulans, lactobacillus acidophilus, yeast mannans are uniformly mixed with bentonite, then with Chinese herb feed additive,
Kelp Powder, glycine betaine, vitamin C, vitamin E and carrier are uniformly mixed.
8. the preparation method of the immunopotentiator according to claim 7 for preventing red head disease of Pseudobagrus fulvidraco, which is characterized in that institute
The preparation method for stating Chinese herb feed additive includes the following steps:The herbal powder after drying is weighed according to the ratio, by solid-liquid ratio 1:
10-15 is heated to boiling 30-40min after soaking 20-50min, and filtrate is collected in filtering;The dregs of a decoction are pressed into solid-liquid ratio 1:5-10
It soaks and boils 15-20min again, filter, collect filtrate;Merging filtrate, concentrated freeze-dried powder processed.
9. a kind of application of the immunopotentiator such as according to any one of claims 1 to 6 preventing red head disease of Pseudobagrus fulvidraco, special
Sign is that the immunopotentiator is used to prepare the feed for preventing red head disease of Pseudobagrus fulvidraco.
10. the application of the immunopotentiator according to claim 9 for preventing red head disease of Pseudobagrus fulvidraco, which is characterized in that described
Immunopotentiator is made an addition to by mass fraction 0.08%-0.15% in the feed of Pelteobagrus fulvidraco.
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CN111557397A (en) * | 2020-05-27 | 2020-08-21 | 浙江明辉饲料有限公司 | Fish feed formula for improving fullness of pelteobagrus fulvidraco fish fees and preparation method thereof |
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WO2023237685A1 (en) * | 2022-06-10 | 2023-12-14 | Dsm Ip Assets B.V. | Combinations comprising vitamin and bacillus coagulans |
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